Rroxscaffold_3G00275430

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000800
Physical Location & Seq
Reverse (-)
66877623 .. 66887859
10237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275430.1

Sequence Viewer

Length: 4059 bp
ATGACCTCCTCCGAAGCCGACGTTCTGATTTCCGACCTCTCCACAGGTAAAGCAGGGATTCATGGACGCATTTCGGCCTACCTGCTTGTGTTCAAGGAACCCCTCAAAACCTGTGCCCAAAAGCAATCTCTTATTACCGATTACTTTCCCTTCCTCAACCGAGCGCTTACTCTTCTCCGGCGAAAGCTAGGTCATCCTTCAAAGTTGAATGAGAAATTCGCCGCCGAATGGTTCGCTGTGTTTCGCGCGTTGGCGTCGTTGGGAGGATGGCGAGAGGAGGGACTAGCAATTTTGAAGGGCATCAAGGAGATGCAACGCCTCCCTGTCAGTGACGTCACATCCAACTTATATTTGCCGGATTTGGATGCCGATGCCGATGATGCAGGGGATGCAGCATTGCTTGCCAAAACGGTGCCGTTGCTGTTTGTTTGCGCAGCAAAGAATAAGAAGCCCAAGCATCCTGACCAGTATCACCGCCTCTATCATATTGCTACCGAGTTTCATACATGGACCAGATCCCCGCCAGCTCGTGCTCGAGGAGCAAGCGAGCTATCTTGCGACTTGTACCATCTTTTTAGTTGGCGAGTGTCTCTCCAGGCTTCAGACACTTTGAAGTTGTATTGTCAGGCAACATGGCTTATAATCGAAGGAAAACTAAAGGAGCCAGACACTAGATTATCAAATTATGACAGCTTGGAATTATTTGGCAAGTGGTGTGAAGCAAGTACTTTACTACTCCAAGTTCTTTGTCAATCTGAGGGTACTCATGAAGTGCAAGGAGTTATAAAGACTGGGCTGAAAAGTGGGCTGCTGAACATTTTTCTAATAGACTGCCAGGTAAAACTCGTAAAAGGTCATGAAAATGGGGATGATGTGGGCACTTTGTTAGGCAATTTGTTTCTATCTTCTAGCAAAGTATCAAGAAAGAAGATATTGCAGCAGAAAGTGTTTCAAGATATTCAGTCTGCCCTTGCTGTGTGGTCGAGCATTCCTACTTCAGATGGCTCCATGGTGCCTGAAAATATAATGAAGATACTTTATTCTGTACTTGATTTGCTATCCCTCAAGGGTCGCGTAGACTTTCACTGTACTATATACAAGGTTATGATTAAGATCTATACCCAGAGAGGAGTCTCGTTGAATAATTTGTTGGCCAAATTGTGGGAATCCAGAAGGATCAGTCATGCACTCTGCCCTTCTCCAGTTCAAGAGGCATTTATGAGCCTGTCAGATGTTTTTGGCGAAAAGTTAAAGTTAACTGAGTTTTGGATAGAGTGTTTTAGTAAATCCCAGCCATTGGTAGTAGGGTTTCATAACAATTTCTCATCTTCTCAATCCAATATAACAGTTCATAATGTTGAAGAAGTAACTTCTGCACTAATTTCAAGTGTTTCTGAATCTGGTTCTTACCTAGCTGGATATATGTACTACGATTTGTGTGAAAAATTGGTTTCAAATGGGCGTTCAATTGAGGCTCTTCCGTATGCAATGGAAGCCCACCGCTTACGAGTAGAACTATTCAAACAGAAATTTCCATGGTCGTTTGAAGGACGCGGTGAAGGGAATCACGTGCTAGTCTGTAGATCAGTTGCTAGTGAACTATGGTCCTTTGATACTAATTCATGTGACTTGGACATCTGCTATATTAGCCCCTGGAATGCACTTCAGTGCTATCTTGAGAGTATTCTTCAGGTAGGGGTTATCCATGAAATACTGGGAAATGGAGCTGAGGCCGAATATTTTTTGGATCGGGGAAAAAGTCTTTCCTGCTCACATAGCTTGACTCTTTTTAACATTGAGAACTATACCATAAGAAGCAGCGTTGGGCTTCAGCAAAAGGAACTTCAAAGTTCCAGGGAACTCTTGAAGGATGGCATAATAGATGGTTTTTGCTCAAAATGCAAAGTGATACTTGAAGTAACTGCTACACATAATCTTGCTGATTTATACCAAAGCCCCATCAGTGAAATTGAGAGTGCATCTGCAGAGAAATTATCTACTGATCTCTACAAAGAAACTCTAGCCAAGCTACATACTTCTGAGTGGAAAAAGTCTATTGCTTTTCTTGAAGAACAAGATGGTACTATTTTAGTGAAAGATGGTAATTCACAACTTGAAGGCAGTATTGAGTGCAATTCTCCCAATACATTGTGTACGAAGGAACTTCCCTGGGATTTGGATTTGCAAAACTGTGTGGTATCATTTGGGGGTGATGTACCATGCATATGCAAAACAATGGGGTGTTGGCATTGTCTTACAGGGAAAGTAACAAAATCTGGCTTAGTGAATGACGCTATAAAGTTGAAGTGGGAACTAGTACACCGTTGTCTTTCACTCAAGCTGCTCACAGGATTAGGAAATCAGTGCTCAGCGGAAACATATAAACCTATATCATCTAATAGGAAGGGAAATCCAGGAGATATGCTCAATGTTGAGCGTGCTGTAATACTTTACAACCTATGTTGGTTGTCTTTGAAGGACCGTAAGAAGGATACCAGCAATAATCCCCATGTGCTGATTGATATTCCTATATCAAAACTAGTGTCTTGGTTGATGCTTTCATTCGTCCTCTGCCGTGAGGTTTCGAGATTGCTGGCTGCTATTTTCTCACTGTCTACCTCAAGTGTGCTTGAAGCTCCATTGTCAACTTCTTGCAAAGATCTCTGTGGACGTCATTGGGGTTCACACATGGCATCAGGAGCACAGAGTTTACCTAGGCTCGCACCAGAATCTATTCTAAACCTTACAGACTTTGAATTATTGTCTTGTTCATCTGCTCCTGCATGGATTATGGTGTCTCGCTTAAACCCTAAGAATCTTCCTATACATATTCTTCTTCCGGTGCATTCAGTACCAGGAGGAGATTCAGATGGTGTTACAATTTCTGGTGAGAATGATGGTGGTCAGTGGCGCTGTCCATGGGGTTCCACTGTGGTTGATGATGTAGCTCTGAATTTTAGAATGCTATTGGAGGAGAATTTCAATTTATCAAATTACGAGTTAGCTTTAAGTCCTGCTGAAAAGAAAGATCATATATATAATGTGTGGGAAGCGAGACGGAGGATTGATGATCGCCTTTGTACATTGCTGAAGTGTGTTATACTCTCCTCCTCTCTATTTACATCTTCATGCACCAGAAACTTGGAAGATTCATGGTTTGGTAGTACTTTGAAATATGTGCTTCTTGGTGAAGTGTCCAACTGCAAGGATCTGGACACGTTATGTGAGAACCTTGTGAGGAAATTGAAATCTCAATTCACGGACATGGATTCTAGTCTTCTGAAAGTCATCGTCCTTGGGGTGTCCAAATATGGCTTTAAAGGAGGCACAGAACAAGTTATGTTGGAGTTTTGCCTTAAGAAAGGTTGTTTCTTTAGTGATGTTCATTTCAAGGATGACCATTGTGCTGATGAATTGCGTGGCTTTGTTTCCAAACTACATGAAGCAGTGAAGAAGGTCAAAGGCTTTAATAGAATTATAAAGCCGATAATCTTAGTCCCAGACTTTGACGTCCAGGGGAAGGTTGGACATGCACCCCCAACTGAAAAACTGGCAGCAGCTTTGGAAAGCAGGGATCTCTTCATATATATAGGCCATGGAGATGGGTCGTCATATATTCCTAATGATAGGATCGAGAGACTCCAAAACTGTGCTGGGGCTTTCCTAATGGAATGCAGTTGTGCTTCTTTGAAACTAGATGGGTGTTATGTTCCACGAGGTCCTCCCCTATCGTATCTGCTTGCCGGTTCTCCTGTCATTCTCGGCAATTTATGGGAAGTAACAGGCCTTGACATTGATAAATTTACACATGCTATACTTGTCTCTTGGTTTAAAGATAGGTCTTACCGTAGAGATTTCAATCAGAAGCTAATGCTTGGAACATGCATGAGTCGAGCCCGTGATAAGTGCAGTCATTTTCCTTGCTTGGTTGGGGCATCACCGGCTATACCTTTGGAGGATTTTGAGATCAAGCCGATGGATGAATCAGAATTAATCGAAGCATTAGAGTCAATGAAAGTAGTTAATGGAAGAATTGACTCTGAGAACATAACATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

1352

Amino Acids

150.63

Weight (kDa)

6.31

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 8 - 82 1.5e-09 Separase-like, TPR repeats
TPR_ESP1 PF25110 92 - 173 1.6e-09 Separase-like, TPR repeats
Separin_C PF03568 1170 - 1265 1.2e-31 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 641, 785, 3479
AasI GACNNNNNNGTC 1 cut(s) 3509
AatII GACGTC 3 cut(s) 336, 2674, 3513
Acc16I TGCGCA 1 cut(s) 433
Acc36I ACCTGC 1 cut(s) 90
AccB1I GGYRCC 2 cut(s) 412, 1012
AccB7I CCANNNNNTGG 2 cut(s) 1161, 1297
AccI GTMKAC 2 cut(s) 1077, 2615
AccII CGCG 4 cut(s) 246, 248, 1074, 1554
AciI CCGC 6 cut(s) 222, 475, 521, 1501, 1554, 2372
AclWI GGATC 6 cut(s) 510, 1184, 1755, 3216, 3582, 3638
AcoI YGGCCR 1 cut(s) 1152
AcsI RAATTY 5 cut(s) 215, 1529, 2953, 2977, 3800
AcuI CTGAAG 6 cut(s) 585, 981, 1649, 1673, 1814, 3110
AcvI CACGTG 1 cut(s) 1570
AcyI GRCGYC 4 cut(s) 254, 333, 2671, 3510
AfeI AGCGCT 1 cut(s) 165
AfiI CCNNNNNNNGG 5 cut(s) 228, 1161, 1297, 2488, 3520
AflII CTTAAG 1 cut(s) 3356
AflIII ACRYGT 1 cut(s) 3216
AhlI ACTAGT 2 cut(s) 2314, 2539
AjnI CCWGG 8 cut(s) 594, 834, 1652, 1853, 2168, 2413, 2854, 3513
AjuI GAANNNNNNNTTGG 2 cut(s) 1133, 1165
AleI CACNNNNGTG 1 cut(s) 1666
Alw21I GWGCWC 3 cut(s) 535, 2369, 2704
Alw26I GTCTC 6 cut(s) 594, 1138, 2802, 3049, 3631, 3826
AlwI GGATC 6 cut(s) 510, 1184, 1755, 3216, 3582, 3638
Ama87I CYCGRG 1 cut(s) 534
Aor51HI AGCGCT 1 cut(s) 165
AoxI GGCC 5 cut(s) 75, 1152, 1731, 3592, 3784
ApeKI GCWGC 9 cut(s) 392, 434, 808, 937, 1818, 2341, 2597, 3554, 3557
ApoI RAATTY 5 cut(s) 215, 1529, 2953, 2977, 3800
ArsI GACNNNNNNTTYG 4 cut(s) 175, 207, 2528, 2560
AseI ATTAAT 1 cut(s) 3992
Asp700I GAANNNNTTC 1 cut(s) 2733
AspA2I CCTAGG 1 cut(s) 2714
AspLEI GCGC 4 cut(s) 166, 248, 434, 2913
AspS9I GGNCC 4 cut(s) 510, 1605, 2479, 3719
AsuHPI GGTGA 6 cut(s) 464, 1568, 2222, 2900, 3200, 3930
AvaI CYCGRG 1 cut(s) 534
AvaII GGWCC 4 cut(s) 510, 1605, 2479, 3719
AvrII CCTAGG 1 cut(s) 2714
BaeGI GKGCMC 2 cut(s) 118, 881
BalI TGGCCA 1 cut(s) 1154
BanI GGYRCC 2 cut(s) 412, 1012
BanII GRGCYC 1 cut(s) 3898
BauI CACGAG 2 cut(s) 528, 3714
BbrPI CACGTG 1 cut(s) 1570
BbsI GAAGAC 1 cut(s) 3269
Bbv12I GWGCWC 3 cut(s) 535, 2369, 2704
BbvCI CCTCAGC 1 cut(s) 1728
BbvI GCAGC 9 cut(s) 404, 446, 795, 949, 1830, 2328, 2584, 3566, 3569
BceAI ACGGC 2 cut(s) 400, 2559
BciT130I CCWGG 8 cut(s) 596, 836, 1654, 1855, 2170, 2415, 2856, 3515
BciVI GTATCC 1 cut(s) 2485
BcoDI GTCTC 6 cut(s) 594, 1138, 2802, 3049, 3631, 3826
BcuI ACTAGT 2 cut(s) 2314, 2539
BfmI CTRYAG 2 cut(s) 1579, 1983
BfoI RGCGCY 2 cut(s) 167, 2914
BfrI CTTAAG 1 cut(s) 3356
BfuAI ACCTGC 1 cut(s) 90
BfuI GTATCC 1 cut(s) 2485
BglII AGATCT 2 cut(s) 1113, 2659
BlnI CCTAGG 1 cut(s) 2714
BlpI GCTNAGC 1 cut(s) 2368
BmcAI AGTACT 2 cut(s) 727, 3166
Bme1390I CCNGG 8 cut(s) 596, 836, 1654, 1855, 2170, 2415, 2856, 3515
Bme18I GGWCC 4 cut(s) 510, 1605, 2479, 3719
BmeT110I CYCGRG 1 cut(s) 534
BmgT120I GGNCC 4 cut(s) 510, 1605, 2479, 3719
BmiI GGNNCC 6 cut(s) 99, 414, 663, 1006, 1014, 2926
BmrFI CCNGG 8 cut(s) 596, 836, 1654, 1855, 2170, 2415, 2856, 3515
BmrI ACTGGG 2 cut(s) 801, 1724
BmuI ACTGGG 2 cut(s) 801, 1724
BpiI GAAGAC 1 cut(s) 3269
BplI GAGNNNNNCTC 4 cut(s) 576, 608, 2409, 2441
BpmI CTGGAG 2 cut(s) 578, 1185
Bpu10I CCTNAGC 1 cut(s) 1728
Bpu1102I GCTNAGC 1 cut(s) 2368
BpuEI CTTGAG 4 cut(s) 1049, 1697, 2321, 2605
BsaAI YACGTR 1 cut(s) 1570
BsaBI GATNNNNATC 2 cut(s) 1112, 3858
BsaHI GRCGYC 4 cut(s) 254, 333, 2671, 3510
BsaWI WCCGGW 1 cut(s) 2839
Bsc4I CCNNNNNNNGG 5 cut(s) 228, 1161, 1297, 2488, 3520
Bse118I RCCGGY 2 cut(s) 3743, 3940
Bse1I ACTGG 5 cut(s) 466, 796, 1202, 1719, 3555
Bse3DI GCAATG 3 cut(s) 395, 1494, 3083
Bse8I GATNNNNATC 2 cut(s) 1112, 3858
BseBI CCWGG 8 cut(s) 596, 836, 1654, 1855, 2170, 2415, 2856, 3515
BseJI GATNNNNATC 2 cut(s) 1112, 3858
BseLI CCNNNNNNNGG 5 cut(s) 228, 1161, 1297, 2488, 3520
BseMI GCAATG 3 cut(s) 395, 1494, 3083
BseMII CTCAG 6 cut(s) 747, 1251, 1719, 2031, 2382, 4032
BseNI ACTGG 5 cut(s) 466, 796, 1202, 1719, 3555
BseRI GAGGAG 7 cut(s) 290, 552, 1143, 2874, 2987, 3097, 3100
BseSI GKGCMC 2 cut(s) 118, 881
BseXI GCAGC 9 cut(s) 404, 446, 795, 949, 1830, 2328, 2584, 3566, 3569
BseYI CCCAGC 2 cut(s) 1290, 3653
BsgI GTGCAG 2 cut(s) 1359, 3928
Bsh1236I CGCG 4 cut(s) 246, 248, 1074, 1554
BshFI GGCC 5 cut(s) 77, 1154, 1733, 3594, 3786
BshNI GGYRCC 2 cut(s) 412, 1012
BsiHKAI GWGCWC 3 cut(s) 535, 2369, 2704
BsiHKCI CYCGRG 1 cut(s) 534
BsiSI CCGG 5 cut(s) 178, 356, 2840, 3744, 3941
BslFI GGGAC 2 cut(s) 294, 3482
BslI CCNNNNNNNGG 5 cut(s) 228, 1161, 1297, 2488, 3520
BsmAI GTCTC 6 cut(s) 594, 1138, 2802, 3049, 3631, 3826
BsmBI CGTCTC 1 cut(s) 3049
BsmFI GGGAC 2 cut(s) 294, 3482
BsmI GAATGC 5 cut(s) 987, 1663, 2845, 2967, 3677
BsnI GGCC 5 cut(s) 77, 1154, 1733, 3594, 3786
BsoBI CYCGRG 1 cut(s) 534
Bsp1286I GDGCHC 6 cut(s) 118, 535, 881, 2369, 2704, 3898
Bsp1407I TGTACA 1 cut(s) 3080
Bsp1720I GCTNAGC 1 cut(s) 2368
Bsp19I CCATGG 4 cut(s) 1008, 1535, 2918, 3595
BspACI CCGC 6 cut(s) 222, 475, 521, 1501, 1554, 2372
BspANI GGCC 5 cut(s) 77, 1154, 1733, 3594, 3786
BspCNI CTCAG 6 cut(s) 748, 1252, 1720, 2032, 2381, 4033
BspFNI CGCG 4 cut(s) 246, 248, 1074, 1554
BspHI TCATGA 2 cut(s) 766, 856
BspLI GGNNCC 6 cut(s) 99, 414, 663, 1006, 1014, 2926
BspMAI CTGCAG 1 cut(s) 1987
BspMI ACCTGC 1 cut(s) 90
BspPI GGATC 6 cut(s) 510, 1184, 1755, 3216, 3582, 3638
BspQI GCTCTTC 1 cut(s) 1482
BspT107I GGYRCC 2 cut(s) 412, 1012
BspTI CTTAAG 1 cut(s) 3356
BsrDI GCAATG 3 cut(s) 395, 1494, 3083
BsrFI RCCGGY 2 cut(s) 3743, 3940
BsrGI TGTACA 1 cut(s) 3080
BsrI ACTGG 5 cut(s) 466, 796, 1202, 1719, 3555
BssAI RCCGGY 2 cut(s) 3743, 3940
BssNI GRCGYC 4 cut(s) 254, 333, 2671, 3510
BssSI CACGAG 2 cut(s) 528, 3714
BssT1I CCWWGG 6 cut(s) 1008, 1535, 2714, 2918, 3295, 3595
Bst2BI CACGAG 2 cut(s) 528, 3714
Bst2UI CCWGG 8 cut(s) 596, 836, 1654, 1855, 2170, 2415, 2856, 3515
Bst6I CTCTTC 3 cut(s) 177, 1482, 3584
BstACI GRCGYC 4 cut(s) 254, 333, 2671, 3510
BstAFI CTTAAG 1 cut(s) 3356
BstAPI GCANNNNNTGC 2 cut(s) 389, 401
BstAUI TGTACA 1 cut(s) 3080
BstBAI YACGTR 1 cut(s) 1570
BstC8I GCNNGC 8 cut(s) 402, 525, 544, 548, 2439, 2595, 2721, 3741
BstDEI CTNAG 9 cut(s) 756, 1260, 1728, 2040, 2281, 2368, 2811, 3493, 4041
BstDSI CCRYGG 4 cut(s) 1008, 1535, 2918, 3595
BstFNI CGCG 4 cut(s) 246, 248, 1074, 1554
BstH2I RGCGCY 2 cut(s) 167, 2914
BstHHI GCGC 4 cut(s) 166, 248, 434, 2913
BstMAI GTCTC 6 cut(s) 594, 1138, 2802, 3049, 3631, 3826
BstNI CCWGG 8 cut(s) 596, 836, 1654, 1855, 2170, 2415, 2856, 3515
BstNSI RCATGY 3 cut(s) 3533, 3812, 3885
BstSCI CCNGG 8 cut(s) 594, 834, 1652, 1853, 2168, 2413, 2854, 3513
BstSFI CTRYAG 2 cut(s) 1579, 1983
BstSLI GKGCMC 2 cut(s) 118, 881
BstUI CGCG 4 cut(s) 246, 248, 1074, 1554
BstV1I GCAGC 9 cut(s) 404, 446, 795, 949, 1830, 2328, 2584, 3566, 3569
BstV2I GAAGAC 1 cut(s) 3269
BstX2I RGATCY 5 cut(s) 515, 1113, 2659, 3208, 3574
BstXI CCANNNNNNTGG 2 cut(s) 3142, 3602
BstYI RGATCY 5 cut(s) 515, 1113, 2659, 3208, 3574
BsuI GTATCC 1 cut(s) 2485
BsuRI GGCC 5 cut(s) 77, 1154, 1733, 3594, 3786
BtgI CCRYGG 4 cut(s) 1008, 1535, 2918, 3595
BtsI GCAGTG 1 cut(s) 3453
BtsIMutI CAGTG 9 cut(s) 334, 1084, 1673, 1969, 2369, 2609, 2912, 2928, 3453
BveI ACCTGC 1 cut(s) 90
Cac8I GCNNGC 8 cut(s) 402, 525, 544, 548, 2439, 2595, 2721, 3741
CciI TCATGA 2 cut(s) 766, 856
CfoI GCGC 4 cut(s) 166, 248, 434, 2913
Cfr10I RCCGGY 2 cut(s) 3743, 3940
Cfr13I GGNCC 4 cut(s) 510, 1605, 2479, 3719
CseI GACGC 4 cut(s) 75, 243, 1560, 2300
CspCI CAANNNNNGTGG 2 cut(s) 2917, 2952
DdeI CTNAG 9 cut(s) 756, 1260, 1728, 2040, 2281, 2368, 2811, 3493, 4041
DraI TTTAAA 2 cut(s) 3319, 3832
DrdI GACNNNNNNGTC 1 cut(s) 3509
DseDI GACNNNNNNGTC 1 cut(s) 3509
EaeI YGGCCR 1 cut(s) 1152
Eam1104I CTCTTC 3 cut(s) 177, 1482, 3584
EarI CTCTTC 3 cut(s) 177, 1482, 3584
Eco130I CCWWGG 6 cut(s) 1008, 1535, 2714, 2918, 3295, 3595
Eco147I AGGCCT 1 cut(s) 3786
Eco24I GRGCYC 1 cut(s) 3898
Eco47I GGWCC 4 cut(s) 510, 1605, 2479, 3719
Eco47III AGCGCT 1 cut(s) 165
Eco57I CTGAAG 6 cut(s) 585, 981, 1649, 1673, 1814, 3110
Eco72I CACGTG 1 cut(s) 1570
Eco88I CYCGRG 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 3719
EcoRII CCWGG 8 cut(s) 594, 834, 1652, 1853, 2168, 2413, 2854, 3513
EcoT14I CCWWGG 6 cut(s) 1008, 1535, 2714, 2918, 3295, 3595
EcoT22I ATGCAT 2 cut(s) 2225, 3887
EcoT38I GRGCYC 1 cut(s) 3898
ErhI CCWWGG 6 cut(s) 1008, 1535, 2714, 2918, 3295, 3595
Esp3I CGTCTC 1 cut(s) 3049
FalI AAGNNNNNCTT 2 cut(s) 1896, 1928
FaqI GGGAC 2 cut(s) 294, 3482
FauI CCCGC 1 cut(s) 528
FauNDI CATATG 1 cut(s) 2225
FblI GTMKAC 2 cut(s) 1077, 2615
FriOI GRGCYC 1 cut(s) 3898
FspI TGCGCA 1 cut(s) 433
GlaI GCGC 4 cut(s) 165, 247, 433, 2912
GsaI CCCAGC 2 cut(s) 1294, 3657
GsuI CTGGAG 2 cut(s) 578, 1185
HaeII RGCGCY 2 cut(s) 167, 2914
HaeIII GGCC 5 cut(s) 77, 1154, 1733, 3594, 3786
HapII CCGG 5 cut(s) 178, 356, 2840, 3744, 3941
HgaI GACGC 4 cut(s) 75, 243, 1560, 2300
HhaI GCGC 4 cut(s) 166, 248, 434, 2913
Hin1I GRCGYC 4 cut(s) 254, 333, 2671, 3510
Hin6I GCGC 4 cut(s) 164, 246, 432, 2911
HinP1I GCGC 4 cut(s) 164, 246, 432, 2911
HincII GTYRAC 2 cut(s) 1257, 2646
HindII GTYRAC 2 cut(s) 1257, 2646
HpaI GTTAAC 1 cut(s) 1257
HpaII CCGG 5 cut(s) 178, 356, 2840, 3744, 3941
HphI GGTGA 6 cut(s) 464, 1568, 2222, 2900, 3200, 3930
Hpy99I CGWCG 2 cut(s) 23, 259
HpyCH4IV ACGT 6 cut(s) 21, 333, 1569, 2671, 3218, 3510
HpyF3I CTNAG 9 cut(s) 756, 1260, 1728, 2040, 2281, 2368, 2811, 3493, 4041
HpySE526I ACGT 6 cut(s) 21, 333, 1569, 2671, 3218, 3510
Hsp92I GRCGYC 4 cut(s) 254, 333, 2671, 3510
HspAI GCGC 4 cut(s) 164, 246, 432, 2911
KspAI GTTAAC 1 cut(s) 1257
LguI GCTCTTC 1 cut(s) 1482
LmnI GCTCC 7 cut(s) 539, 661, 1010, 1724, 2641, 2699, 2782
Lsp1109I GCAGC 9 cut(s) 404, 446, 795, 949, 1830, 2328, 2584, 3566, 3569
MaeII ACGT 6 cut(s) 21, 333, 1569, 2671, 3218, 3510
MaeIII GTNAC 8 cut(s) 329, 334, 1366, 1625, 1918, 2266, 2875, 3778
MfeI CAATTG 1 cut(s) 1467
MflI RGATCY 5 cut(s) 515, 1113, 2659, 3208, 3574
MhlI GDGCHC 6 cut(s) 118, 535, 881, 2369, 2704, 3898
MlsI TGGCCA 1 cut(s) 1154
MluNI TGGCCA 1 cut(s) 1154
MlyI GAGTC 6 cut(s) 1140, 1777, 3633, 3898, 4016, 4031
MmeI TCCRAC 5 cut(s) 57, 366, 3222, 3324, 3505
Mox20I TGGCCA 1 cut(s) 1154
Mph1103I ATGCAT 2 cut(s) 2225, 3887
MroXI GAANNNNTTC 1 cut(s) 2733
MscI TGGCCA 1 cut(s) 1154
MslI CAYNNNNRTG 6 cut(s) 861, 1666, 2224, 3127, 3263, 3600
Msp20I TGGCCA 1 cut(s) 1154
MspA1I CMGCKG 1 cut(s) 2372
MspCI CTTAAG 1 cut(s) 3356
MspI CCGG 5 cut(s) 178, 356, 2840, 3744, 3941
MspR9I CCNGG 8 cut(s) 596, 836, 1654, 1855, 2170, 2415, 2856, 3515
MunI CAATTG 1 cut(s) 1467
Mva1269I GAATGC 5 cut(s) 987, 1663, 2845, 2967, 3677
MvaI CCWGG 8 cut(s) 596, 836, 1654, 1855, 2170, 2415, 2856, 3515
MvnI CGCG 4 cut(s) 246, 248, 1074, 1554
NcoI CCATGG 4 cut(s) 1008, 1535, 2918, 3595
NdeI CATATG 1 cut(s) 2225
NlaIV GGNNCC 6 cut(s) 99, 414, 663, 1006, 1014, 2926
NmeAIII GCCGAG 1 cut(s) 3741
NmuCI GTSAC 3 cut(s) 329, 334, 1625
NsbI TGCGCA 1 cut(s) 433
NsiI ATGCAT 2 cut(s) 2225, 3887
NspI RCATGY 3 cut(s) 3533, 3812, 3885
OliI CACNNNNGTG 1 cut(s) 1666
PaeR7I CTCGAG 1 cut(s) 534
PagI TCATGA 2 cut(s) 766, 856
PasI CCCWGGG 1 cut(s) 2169
PceI AGGCCT 1 cut(s) 3786
PciSI GCTCTTC 1 cut(s) 1482
PctI GAATGC 5 cut(s) 987, 1663, 2845, 2967, 3677
PdmI GAANNNNTTC 1 cut(s) 2733
PflMI CCANNNNNTGG 2 cut(s) 1161, 1297
PfoI TCCNGGA 1 cut(s) 2413
PleI GAGTC 6 cut(s) 1139, 1777, 3633, 3897, 4015, 4031
PmaCI CACGTG 1 cut(s) 1570
PmlI CACGTG 1 cut(s) 1570
PpsI GAGTC 6 cut(s) 1139, 1777, 3633, 3897, 4015, 4031
Ppu21I YACGTR 1 cut(s) 1570
PpuMI RGGWCCY 1 cut(s) 3719
PshBI ATTAAT 1 cut(s) 3992
PsiI TTATAA 3 cut(s) 641, 785, 3479
Psp5II RGGWCCY 1 cut(s) 3719
Psp6I CCWGG 8 cut(s) 594, 834, 1652, 1853, 2168, 2413, 2854, 3513
PspCI CACGTG 1 cut(s) 1570
PspFI CCCAGC 2 cut(s) 1290, 3653
PspGI CCWGG 8 cut(s) 594, 834, 1652, 1853, 2168, 2413, 2854, 3513
PspN4I GGNNCC 6 cut(s) 99, 414, 663, 1006, 1014, 2926
PspPI GGNCC 4 cut(s) 510, 1605, 2479, 3719
PspPPI RGGWCCY 1 cut(s) 3719
PspXI VCTCGAGB 1 cut(s) 534
PstI CTGCAG 1 cut(s) 1987
PsuI RGATCY 5 cut(s) 515, 1113, 2659, 3208, 3574
RseI CAYNNNNRTG 6 cut(s) 861, 1666, 2224, 3127, 3263, 3600
SapI GCTCTTC 1 cut(s) 1482
Sau96I GGNCC 4 cut(s) 510, 1605, 2479, 3719
ScaI AGTACT 2 cut(s) 727, 3166
SchI GAGTC 6 cut(s) 1140, 1777, 3633, 3898, 4016, 4031
ScrFI CCNGG 8 cut(s) 596, 836, 1654, 1855, 2170, 2415, 2856, 3515
SduI GDGCHC 6 cut(s) 118, 535, 881, 2369, 2704, 3898
SfcI CTRYAG 2 cut(s) 1579, 1983
Sfr274I CTCGAG 1 cut(s) 534
SinI GGWCC 4 cut(s) 510, 1605, 2479, 3719
SlaI CTCGAG 1 cut(s) 534
SmiMI CAYNNNNRTG 6 cut(s) 861, 1666, 2224, 3127, 3263, 3600
SmlI CTYRAG 6 cut(s) 534, 1064, 1676, 2336, 2620, 3356
SmoI CTYRAG 6 cut(s) 534, 1064, 1676, 2336, 2620, 3356
SpeI ACTAGT 2 cut(s) 2314, 2539
SseBI AGGCCT 1 cut(s) 3786
SsiI CCGC 6 cut(s) 222, 475, 521, 1501, 1554, 2372
SspI AATATT 1 cut(s) 1739
StuI AGGCCT 1 cut(s) 3786
StyD4I CCNGG 8 cut(s) 594, 834, 1652, 1853, 2168, 2413, 2854, 3513
StyI CCWWGG 6 cut(s) 1008, 1535, 2714, 2918, 3295, 3595
TaiI ACGT 6 cut(s) 24, 336, 1572, 2674, 3221, 3513
TaqI TCGA 7 cut(s) 535, 645, 983, 2585, 3633, 3892, 3996
TatI WGTACW 7 cut(s) 725, 1045, 1088, 1425, 2317, 3080, 3164
TauI GCSGC 1 cut(s) 224
TscAI CASTG 9 cut(s) 334, 1091, 1673, 1969, 2369, 2616, 2912, 2935, 3453
TseFI GTSAC 3 cut(s) 329, 334, 1625
TseI GCWGC 9 cut(s) 392, 434, 808, 937, 1818, 2341, 2597, 3554, 3557
Tsp45I GTSAC 3 cut(s) 329, 334, 1625
TspGWI ACGGA 3 cut(s) 1470, 3073, 3275
TspRI CASTG 9 cut(s) 334, 1091, 1673, 1969, 2369, 2616, 2912, 2935, 3453
Van91I CCANNNNNTGG 2 cut(s) 1161, 1297
Vha464I CTTAAG 1 cut(s) 3356
VpaK11BI GGWCC 4 cut(s) 510, 1605, 2479, 3719
VspI ATTAAT 1 cut(s) 3992
XapI RAATTY 5 cut(s) 215, 1529, 2953, 2977, 3800
XceI RCATGY 3 cut(s) 3533, 3812, 3885
XcmI CCANNNNNNNNNTGG 2 cut(s) 3521, 3650
XhoI CTCGAG 1 cut(s) 534
XmaJI CCTAGG 1 cut(s) 2714
XmiI GTMKAC 2 cut(s) 1077, 2615
XmnI GAANNNNTTC 1 cut(s) 2733
ZraI GACGTC 3 cut(s) 334, 2672, 3511
ZrmI AGTACT 2 cut(s) 727, 3166
Zsp2I ATGCAT 2 cut(s) 2225, 3887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.