Rw7G000860

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000800
Physical Location & Seq
Forward (+)
678091 .. 680239
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000860.1

Sequence Viewer

Length: 1479 bp
ATGCAAAATGACAACCCCAACTTTTGTAAGAGAATGCAAATGATGATTAAGGATTTACTAGAGAAAGATGGGTTTGGCACACTAATTGATTGGTTAGAGGAATCAAGCTGTTTAAGACTGTGTGGAGTCAGAGGTCCAAAATACTGCACTCAATGCTTGTCACAAGCACTTGAATTTGCTTCTGGCCATCAATTAGCTGTGGCATATTGTTTACGATCACTTTGCACAGAGGAATATGAACCAAACTCCGAGAAAGTGTTTCAAGATATTCAGTCTGCCCTTGCTGTGTGGTCGAGCATTCCTACTTCAGATGGCTCCATGGTGCCTGAAAATATAATGAAGATACTTTATTCTGTAATTGATTTGCTATCCCTCAAGGGTCGTGTAGACTTTCACTCTACTATATACAAGGTTATGATTAAGATCTATACCCAGAGAGGAGTCTCGTTGAATAATTTGTTGGCCAAATTGTGGGAATCCAGAAGGATCAGTCATGCACTCTGCCCTTCTCCAGTTCAAGAGGCATTTATGAGCCTGTCAGATGTTTTTGGCGAAAAGTTAAAGTTAACTGAGTTTTGGATAGAGTGCTTTAGTAAATCCCAGCCATTGGTAGTAGGGTTTCATAACAATTTCTCATCTTCTCAATCCAATATAACAGTTCATAATGTTGAAGAAGTAACTTCTGCACTAATTTCAAGTGTTTCTGAATCTGGTTCTTACCTAGCTGGATATATGTACTACGATTTGTGTGAAAAATTGGTTTCAAATGGGCGTTCAATTGAGGCTCTTCCGTATGCAATGGAAGCCCACCGCTTACGAGTAGAACTATTCAAACAGAAATTTCCATGGTCGTTTGAAGGACGCGGTGAAGGGAATCACGTGCTAGTCTGTAGATCAGTTGCTAGTGAACTATGGTCCTTTGATACTAGTTCATGTGACTTGGACATCTGCTATATTAGCCCCTGGAATGCCCTTCAGTGCTATCTTGAGAGCATTCTTCAGGTAGGGGTTATCCATGAAATACTGGGAAATGGAGCTGAGGCCGAATATTTTTTGGATCGGGGAAAAAGTATTTCCTGCTCACATAGCTTGACTCTTTTTAACATTGTTTTTTCTTCCTTTTTGGGAGAACTATACCATAAGAAGCAGCGTTGGGCTTCAGCAAAAAAGGAACTTCAAAGTTCCAGGGAACTCTTGAAGGATGGCATAATAGATGGTTTTTGCTCAAAATGCATAGTGATACTTGAAGTAACTGTTACACATAATCTTGCTGATTTATACCAAAGCCCCATCAGTGAAATTGAGAGTGCATCTGCAGAGAAATTATCTACTGATCTCTACAAAGAAACTCTAGCCAAGCTACATACTTCTGAGTGGAAAAAGTCTATTGCTTGTCTTGAAGAACAAGATGGTACTATTTTAGTCAAAGATGGTAATTCACAACTTGAAGGCAGTATTGAGTGCAATTCTCCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

492

Amino Acids

55.33

Weight (kDa)

5.3

Isoelectric Point (pI)

57.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 322
AccB7I CCANNNNNTGG 2 cut(s) 471, 607
AccI GTMKAC 1 cut(s) 387
AccII CGCG 1 cut(s) 864
AciI CCGC 2 cut(s) 811, 864
AclWI GGATC 2 cut(s) 494, 1065
AcoI YGGCCR 2 cut(s) 184, 462
AcsI RAATTY 2 cut(s) 173, 839
AcuI CTGAAG 4 cut(s) 291, 959, 983, 1143
AcvI CACGTG 1 cut(s) 880
AfaI GTAC 2 cut(s) 737, 1414
AfiI CCNNNNNNNGG 2 cut(s) 471, 607
AhlI ACTAGT 1 cut(s) 926
AjnI CCWGG 2 cut(s) 962, 1184
AjuI GAANNNNNNNTTGG 2 cut(s) 443, 475
AluBI AGCT 6 cut(s) 108, 197, 725, 1037, 1089, 1360
AluI AGCT 6 cut(s) 108, 197, 725, 1037, 1089, 1360
Alw26I GTCTC 1 cut(s) 448
AlwI GGATC 2 cut(s) 494, 1065
AoxI GGCC 3 cut(s) 184, 462, 1041
ApeKI GCWGC 1 cut(s) 1147
ApoI RAATTY 2 cut(s) 173, 839
AspS9I GGNCC 2 cut(s) 134, 915
AsuHPI GGTGA 1 cut(s) 878
AvaII GGWCC 2 cut(s) 134, 915
BalI TGGCCA 2 cut(s) 186, 464
BanI GGYRCC 1 cut(s) 322
BbrPI CACGTG 1 cut(s) 880
BbvCI CCTCAGC 1 cut(s) 1038
BbvI GCAGC 1 cut(s) 1159
BccI CCATC 8 cut(s) 62, 195, 305, 1196, 1208, 1298, 1403, 1424
BcgI CGANNNNNNTGC 2 cut(s) 204, 238
BciT130I CCWGG 2 cut(s) 964, 1186
BcoDI GTCTC 1 cut(s) 448
BcuI ACTAGT 1 cut(s) 926
BfaI CTAG 6 cut(s) 59, 722, 884, 903, 927, 1352
BfmI CTRYAG 2 cut(s) 889, 1314
BglII AGATCT 1 cut(s) 423
BisI GCNGC 1 cut(s) 1148
BlsI GCNGC 1 cut(s) 1149
Bme1390I CCNGG 2 cut(s) 964, 1186
Bme18I GGWCC 2 cut(s) 134, 915
BmgT120I GGNCC 2 cut(s) 134, 915
BmiI GGNNCC 2 cut(s) 316, 324
BmrFI CCNGG 2 cut(s) 964, 1186
BmrI ACTGGG 1 cut(s) 1034
BmsI GCATC 1 cut(s) 1319
BmuI ACTGGG 1 cut(s) 1034
BpmI CTGGAG 1 cut(s) 495
Bpu10I CCTNAGC 1 cut(s) 1038
BpuEI CTTGAG 2 cut(s) 359, 1007
BsaAI YACGTR 1 cut(s) 880
BsaBI GATNNNNATC 1 cut(s) 422
BsaJI CCNNGG 4 cut(s) 318, 845, 962, 1185
Bsc4I CCNNNNNNNGG 2 cut(s) 471, 607
Bse1I ACTGG 2 cut(s) 512, 1029
Bse3DI GCAATG 1 cut(s) 804
Bse8I GATNNNNATC 1 cut(s) 422
BseBI CCWGG 2 cut(s) 964, 1186
BseDI CCNNGG 4 cut(s) 318, 845, 962, 1185
BseGI GGATG 1 cut(s) 1207
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 2 cut(s) 471, 607
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 3 cut(s) 561, 1029, 1362
BseNI ACTGG 2 cut(s) 512, 1029
BseRI GAGGAG 1 cut(s) 453
BseXI GCAGC 1 cut(s) 1159
BseYI CCCAGC 1 cut(s) 600
BsgI GTGCAG 2 cut(s) 130, 669
Bsh1236I CGCG 1 cut(s) 864
BshFI GGCC 3 cut(s) 186, 464, 1043
BshNI GGYRCC 1 cut(s) 322
BslI CCNNNNNNNGG 2 cut(s) 471, 607
BsmAI GTCTC 1 cut(s) 448
BsmI GAATGC 4 cut(s) 39, 297, 973, 993
BsnI GGCC 3 cut(s) 186, 464, 1043
Bsp143I GATC 6 cut(s) 215, 423, 486, 893, 1057, 1333
Bsp19I CCATGG 2 cut(s) 318, 845
BspACI CCGC 2 cut(s) 811, 864
BspANI GGCC 3 cut(s) 186, 464, 1043
BspCNI CTCAG 3 cut(s) 562, 1030, 1363
BspFNI CGCG 1 cut(s) 864
BspLI GGNNCC 2 cut(s) 316, 324
BspMAI CTGCAG 1 cut(s) 1318
BspPI GGATC 2 cut(s) 494, 1065
BspQI GCTCTTC 1 cut(s) 792
BspT107I GGYRCC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 804
BsrI ACTGG 2 cut(s) 512, 1029
BssECI CCNNGG 4 cut(s) 318, 845, 962, 1185
BssMI GATC 6 cut(s) 215, 423, 486, 893, 1057, 1333
BssT1I CCWWGG 2 cut(s) 318, 845
Bst2UI CCWGG 2 cut(s) 964, 1186
Bst4CI ACNGT 3 cut(s) 120, 658, 1255
Bst6I CTCTTC 1 cut(s) 792
BstAPI GCANNNNNTGC 1 cut(s) 153
BstBAI YACGTR 1 cut(s) 880
BstDEI CTNAG 3 cut(s) 570, 1038, 1371
BstDSI CCRYGG 2 cut(s) 318, 845
BstF5I GGATG 1 cut(s) 1207
BstFNI CGCG 1 cut(s) 864
BstKTI GATC 6 cut(s) 218, 426, 489, 896, 1060, 1336
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 6 cut(s) 215, 423, 486, 893, 1057, 1333
BstMWI GCNNNNNNNGC 5 cut(s) 153, 803, 957, 1086, 1230
BstNI CCWGG 2 cut(s) 964, 1186
BstSCI CCNGG 2 cut(s) 962, 1184
BstSFI CTRYAG 2 cut(s) 889, 1314
BstUI CGCG 1 cut(s) 864
BstV1I GCAGC 1 cut(s) 1159
BstX2I RGATCY 1 cut(s) 423
BstYI RGATCY 1 cut(s) 423
BsuRI GGCC 3 cut(s) 186, 464, 1043
BtgI CCRYGG 2 cut(s) 318, 845
BtsCI GGATG 1 cut(s) 1207
BtsIMutI CAGTG 2 cut(s) 983, 1300
Cfr13I GGNCC 2 cut(s) 134, 915
CseI GACGC 1 cut(s) 870
Csp6I GTAC 2 cut(s) 736, 1413
CviAII CATG 5 cut(s) 319, 494, 846, 933, 1016
CviQI GTAC 2 cut(s) 736, 1413
DdeI CTNAG 3 cut(s) 570, 1038, 1371
DpnI GATC 6 cut(s) 217, 425, 488, 895, 1059, 1335
DpnII GATC 6 cut(s) 215, 423, 486, 893, 1057, 1333
EaeI YGGCCR 2 cut(s) 184, 462
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Eco130I CCWWGG 2 cut(s) 318, 845
Eco47I GGWCC 2 cut(s) 134, 915
Eco57I CTGAAG 4 cut(s) 291, 959, 983, 1143
Eco72I CACGTG 1 cut(s) 880
EcoRII CCWGG 2 cut(s) 962, 1184
EcoT14I CCWWGG 2 cut(s) 318, 845
EcoT22I ATGCAT 1 cut(s) 1235
ErhI CCWWGG 2 cut(s) 318, 845
FaeI CATG 5 cut(s) 322, 497, 849, 936, 1019
FatI CATG 5 cut(s) 318, 493, 845, 932, 1015
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 1 cut(s) 1148
FokI GGATG 1 cut(s) 1214
Fsp4HI GCNGC 1 cut(s) 1148
FspBI CTAG 6 cut(s) 59, 722, 884, 903, 927, 1352
GluI GCNGC 1 cut(s) 1148
GsaI CCCAGC 1 cut(s) 604
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 3 cut(s) 186, 464, 1043
HgaI GACGC 1 cut(s) 870
Hin1II CATG 5 cut(s) 322, 497, 849, 936, 1019
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HinfI GANTC 7 cut(s) 101, 126, 441, 476, 707, 874, 1093
HpaI GTTAAC 1 cut(s) 567
HphI GGTGA 1 cut(s) 878
Hpy166II GTNNAC 4 cut(s) 212, 388, 567, 908
Hpy188I TCNGA 6 cut(s) 131, 250, 310, 541, 706, 1372
Hpy188III TCNNGA 6 cut(s) 263, 480, 518, 986, 1195, 1397
Hpy8I GTNNAC 4 cut(s) 212, 388, 567, 908
HpyAV CCTTC 7 cut(s) 477, 516, 851, 863, 983, 1192, 1442
HpyCH4III ACNGT 3 cut(s) 120, 658, 1255
HpyCH4IV ACGT 1 cut(s) 879
HpyF10VI GCNNNNNNNGC 5 cut(s) 153, 803, 957, 1086, 1230
HpyF3I CTNAG 3 cut(s) 570, 1038, 1371
HpySE526I ACGT 1 cut(s) 879
Hsp92II CATG 5 cut(s) 322, 497, 849, 936, 1019
KspAI GTTAAC 1 cut(s) 567
Kzo9I GATC 6 cut(s) 215, 423, 486, 893, 1057, 1333
LguI GCTCTTC 1 cut(s) 792
LmnI GCTCC 2 cut(s) 320, 1034
Lsp1109I GCAGC 1 cut(s) 1159
LweI GCATC 1 cut(s) 1319
MaeI CTAG 6 cut(s) 59, 722, 884, 903, 927, 1352
MaeII ACGT 1 cut(s) 879
MaeIII GTNAC 5 cut(s) 159, 676, 935, 1249, 1255
MalI GATC 6 cut(s) 217, 425, 488, 895, 1059, 1335
MboI GATC 6 cut(s) 215, 423, 486, 893, 1057, 1333
MboII GAAGA 7 cut(s) 352, 630, 683, 779, 989, 1107, 1412
MfeI CAATTG 1 cut(s) 777
MflI RGATCY 1 cut(s) 423
MlsI TGGCCA 2 cut(s) 186, 464
MluNI TGGCCA 2 cut(s) 186, 464
MlyI GAGTC 3 cut(s) 135, 450, 1087
MnlI CCTC 8 cut(s) 91, 125, 223, 383, 431, 514, 775, 1033
Mox20I TGGCCA 2 cut(s) 186, 464
Mph1103I ATGCAT 1 cut(s) 1235
MscI TGGCCA 2 cut(s) 186, 464
MseI TTAA 7 cut(s) 48, 113, 420, 560, 566, 1101, 1477
Msp20I TGGCCA 2 cut(s) 186, 464
MspR9I CCNGG 2 cut(s) 964, 1186
MunI CAATTG 1 cut(s) 777
Mva1269I GAATGC 4 cut(s) 39, 297, 973, 993
MvaI CCWGG 2 cut(s) 964, 1186
MvnI CGCG 1 cut(s) 864
MwoI GCNNNNNNNGC 5 cut(s) 153, 803, 957, 1086, 1230
NcoI CCATGG 2 cut(s) 318, 845
NdeII GATC 6 cut(s) 215, 423, 486, 893, 1057, 1333
NlaIII CATG 5 cut(s) 322, 497, 849, 936, 1019
NlaIV GGNNCC 2 cut(s) 316, 324
NmuCI GTSAC 2 cut(s) 159, 935
NsiI ATGCAT 1 cut(s) 1235
PciSI GCTCTTC 1 cut(s) 792
PctI GAATGC 4 cut(s) 39, 297, 973, 993
PfeI GAWTC 4 cut(s) 101, 476, 707, 874
PflMI CCANNNNNTGG 2 cut(s) 471, 607
PkrI GCNGC 1 cut(s) 1149
PleI GAGTC 3 cut(s) 134, 449, 1087
PmaCI CACGTG 1 cut(s) 880
PmlI CACGTG 1 cut(s) 880
PpsI GAGTC 3 cut(s) 134, 449, 1087
Ppu21I YACGTR 1 cut(s) 880
Psp6I CCWGG 2 cut(s) 962, 1184
PspCI CACGTG 1 cut(s) 880
PspFI CCCAGC 1 cut(s) 600
PspGI CCWGG 2 cut(s) 962, 1184
PspN4I GGNNCC 2 cut(s) 316, 324
PspPI GGNCC 2 cut(s) 134, 915
PstI CTGCAG 1 cut(s) 1318
PsuI RGATCY 1 cut(s) 423
RsaI GTAC 2 cut(s) 737, 1414
RsaNI GTAC 2 cut(s) 736, 1413
SapI GCTCTTC 1 cut(s) 792
SaqAI TTAA 7 cut(s) 48, 113, 420, 560, 566, 1101, 1477
SatI GCNGC 1 cut(s) 1148
Sau3AI GATC 6 cut(s) 215, 423, 486, 893, 1057, 1333
Sau96I GGNCC 2 cut(s) 134, 915
SchI GAGTC 3 cut(s) 135, 450, 1087
ScrFI CCNGG 2 cut(s) 964, 1186
SfaNI GCATC 1 cut(s) 1319
SfcI CTRYAG 2 cut(s) 889, 1314
SinI GGWCC 2 cut(s) 134, 915
SmlI CTYRAG 2 cut(s) 374, 986
SmoI CTYRAG 2 cut(s) 374, 986
SpeI ACTAGT 1 cut(s) 926
SsiI CCGC 2 cut(s) 811, 864
SspI AATATT 1 cut(s) 1049
SspMI CTAG 6 cut(s) 59, 722, 884, 903, 927, 1352
StyD4I CCNGG 2 cut(s) 962, 1184
StyI CCWWGG 2 cut(s) 318, 845
TaaI ACNGT 3 cut(s) 120, 658, 1255
TaiI ACGT 1 cut(s) 882
TaqI TCGA 1 cut(s) 293
TatI WGTACW 1 cut(s) 735
TfiI GAWTC 4 cut(s) 101, 476, 707, 874
Tru1I TTAA 7 cut(s) 48, 113, 420, 560, 566, 1101, 1477
Tru9I TTAA 7 cut(s) 48, 113, 420, 560, 566, 1101, 1477
TscAI CASTG 2 cut(s) 983, 1300
TseFI GTSAC 2 cut(s) 159, 935
TseI GCWGC 1 cut(s) 1147
Tsp45I GTSAC 2 cut(s) 159, 935
TspDTI ATGAA 6 cut(s) 252, 353, 611, 650, 921, 1032
TspGWI ACGGA 1 cut(s) 780
TspRI CASTG 2 cut(s) 983, 1300
Van91I CCANNNNNTGG 2 cut(s) 471, 607
VpaK11BI GGWCC 2 cut(s) 134, 915
XapI RAATTY 2 cut(s) 173, 839
XmiI GTMKAC 1 cut(s) 387
XspI CTAG 6 cut(s) 59, 722, 884, 903, 927, 1352
Zsp2I ATGCAT 1 cut(s) 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.