Rw7G030790

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000800
Physical Location & Seq
Reverse (-)
43701772 .. 43703589
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G030790.1

Sequence Viewer

Length: 789 bp
ATGGCATCACGTAATGAATCTAATGGTGGCGAGAACTTGTCTGAGTTGGCCAACAAACTAGTATTAGAAGCAGTGAATGAGCTTGAAGGTTTACATTCTTTTGTAAATATAGAGCGAACAATATTAGTTCTGGACTACGAGGTGCAGGTATGTGCCAGATTCCCTATCATTTCGGCAACAACCAATAGAAACTACCACAATCAATATCATATTCAGAGTATTATGACTTCATTTCCTTTGATAGATCCCTTGGATGCCTTTTATCTTCTGAATCCCAGTGGTGATCTAAATTATACACAAAATGAATTTGAGACATGGTTTAGAGATCAAAACTTGGAGGGCAAGGCTGGATGTGCACCTCCTGCTGAAGAATTGGAAAAGGCAACAATAATACAATATATTCCTAGACTTGAGATTCAGAGACTGGAACACTGTGCCTCAACTCTCCTGATGGGATGCAGTAGTGGTTCTCTGAAACTGAATGGATGTTATGTTCCACAAGGTACTCCACTATCCTATCTGCTGGCTGGTTCTCCTGATACTAACCGATTTGCAAATTCCATGCTTGATTCTTGGTTGAAAGCAAGTTCGAGTTACTGTGAGGGTTGTGCCCAAAGCAACAAGAAAGTCGCAAAGAAGAAATTGGCCACACACCCGTCTTGTGAGCATAGGCCGAAGGTTGGATCATTCATGAGTGAAGCACGTAAAACTTGCCAGTTACCTTTCTTGATTGGAGCAGCACCGGTATGCTATGGTGTCCTAACTGACATATGGAACAAGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

262

Amino Acids

29.13

Weight (kDa)

6.09

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Separin_C PF03568 81 - 179 5.8e-25 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 136
AclWI GGATC 2 cut(s) 239, 691
AcoI YGGCCR 2 cut(s) 48, 645
AcsI RAATTY 2 cut(s) 305, 556
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 1 cut(s) 505
AfiI CCNNNNNNNGG 1 cut(s) 680
AgeI ACCGGT 1 cut(s) 742
AgsI TTSAA 2 cut(s) 86, 580
AhlI ACTAGT 1 cut(s) 58
AloI GAACNNNNNNTCC 2 cut(s) 477, 509
AluBI AGCT 1 cut(s) 82
AluI AGCT 1 cut(s) 82
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 2 cut(s) 305, 415
Alw44I GTGCAC 1 cut(s) 354
AlwI GGATC 2 cut(s) 239, 691
AlwNI CAGNNNCTG 1 cut(s) 424
AoxI GGCC 3 cut(s) 48, 645, 671
ApaLI GTGCAC 1 cut(s) 354
ApeKI GCWGC 1 cut(s) 737
ApoI RAATTY 2 cut(s) 305, 556
AsiGI ACCGGT 1 cut(s) 742
AsuHPI GGTGA 1 cut(s) 293
BaeGI GKGCMC 2 cut(s) 358, 613
BalI TGGCCA 2 cut(s) 50, 647
BarI GAAGNNNNNNTAC 2 cut(s) 211, 243
Bbv12I GWGCWC 1 cut(s) 358
BbvI GCAGC 1 cut(s) 749
BccI CCATC 1 cut(s) 445
BcoDI GTCTC 2 cut(s) 305, 415
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 2 cut(s) 59, 405
BfuAI ACCTGC 1 cut(s) 136
BisI GCNGC 1 cut(s) 738
BlsI GCNGC 1 cut(s) 739
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 3 cut(s) 14, 244, 446
BmuI ACTGGG 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 431
BsaAI YACGTR 2 cut(s) 11, 704
BsaJI CCNNGG 1 cut(s) 249
BsaWI WCCGGW 1 cut(s) 742
Bsc4I CCNNNNNNNGG 1 cut(s) 680
Bse118I RCCGGY 1 cut(s) 742
Bse1I ACTGG 3 cut(s) 276, 429, 715
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 4 cut(s) 259, 356, 461, 491
BseLI CCNNNNNNNGG 1 cut(s) 680
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 3 cut(s) 276, 429, 715
BseSI GKGCMC 2 cut(s) 358, 613
BseXI GCAGC 1 cut(s) 749
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 3 cut(s) 50, 647, 673
BshTI ACCGGT 1 cut(s) 742
BsiHKAI GWGCWC 1 cut(s) 358
BsiSI CCGG 1 cut(s) 743
BslI CCNNNNNNNGG 1 cut(s) 680
BsmAI GTCTC 2 cut(s) 305, 415
BsnI GGCC 3 cut(s) 50, 647, 673
Bsp1286I GDGCHC 2 cut(s) 358, 613
Bsp143I GATC 4 cut(s) 244, 283, 325, 683
BspANI GGCC 3 cut(s) 50, 647, 673
BspCNI CTCAG 1 cut(s) 34
BspHI TCATGA 1 cut(s) 690
BspMI ACCTGC 1 cut(s) 136
BspPI GGATC 2 cut(s) 239, 691
BsrFI RCCGGY 1 cut(s) 742
BsrI ACTGG 3 cut(s) 276, 429, 715
BssAI RCCGGY 1 cut(s) 742
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 4 cut(s) 244, 283, 325, 683
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 434, 599
BstAPI GCANNNNNTGC 1 cut(s) 362
BstBAI YACGTR 2 cut(s) 11, 704
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 1 cut(s) 42
BstF5I GGATG 4 cut(s) 259, 356, 461, 491
BstKTI GATC 4 cut(s) 247, 286, 328, 686
BstMAI GTCTC 2 cut(s) 305, 415
BstMBI GATC 4 cut(s) 244, 283, 325, 683
BstMWI GCNNNNNNNGC 2 cut(s) 353, 362
BstSLI GKGCMC 2 cut(s) 358, 613
BstV1I GCAGC 1 cut(s) 749
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
BsuRI GGCC 3 cut(s) 50, 647, 673
BtsCI GGATG 4 cut(s) 259, 356, 461, 491
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 3 cut(s) 78, 283, 430
BveI ACCTGC 1 cut(s) 136
Cac8I GCNNGC 1 cut(s) 525
CaiI CAGNNNCTG 1 cut(s) 424
CciI TCATGA 1 cut(s) 690
Cfr10I RCCGGY 1 cut(s) 742
Csp6I GTAC 1 cut(s) 504
CspAI ACCGGT 1 cut(s) 742
CviAII CATG 3 cut(s) 315, 562, 691
CviJI RGCY 6 cut(s) 50, 82, 347, 527, 647, 673
CviKI_1 RGCY 6 cut(s) 50, 82, 347, 527, 647, 673
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 1 cut(s) 42
DpnI GATC 4 cut(s) 246, 285, 327, 685
DpnII GATC 4 cut(s) 244, 283, 325, 683
EaeI YGGCCR 2 cut(s) 48, 645
Eco130I CCWWGG 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 387
EcoT14I CCWWGG 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 3 cut(s) 318, 565, 694
FatI CATG 3 cut(s) 314, 561, 690
FauNDI CATATG 1 cut(s) 770
Fnu4HI GCNGC 1 cut(s) 738
FokI GGATG 4 cut(s) 266, 363, 468, 498
Fsp4HI GCNGC 1 cut(s) 738
FspBI CTAG 2 cut(s) 59, 405
GluI GCNGC 1 cut(s) 738
HaeIII GGCC 3 cut(s) 50, 647, 673
HapII CCGG 1 cut(s) 743
Hin1II CATG 3 cut(s) 318, 565, 694
HinfI GANTC 5 cut(s) 17, 159, 271, 415, 569
HpaII CCGG 1 cut(s) 743
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 2 cut(s) 92, 356
Hpy188I TCNGA 5 cut(s) 43, 216, 270, 420, 474
Hpy188III TCNNGA 5 cut(s) 131, 448, 536, 691, 727
Hpy8I GTNNAC 2 cut(s) 92, 356
HpyAV CCTTC 2 cut(s) 80, 670
HpyCH4III ACNGT 2 cut(s) 434, 599
HpyCH4IV ACGT 2 cut(s) 10, 703
HpyCH4V TGCA 4 cut(s) 145, 356, 459, 554
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 362
HpyF3I CTNAG 1 cut(s) 42
HpySE526I ACGT 2 cut(s) 10, 703
Hsp92II CATG 3 cut(s) 318, 565, 694
Kzo9I GATC 4 cut(s) 244, 283, 325, 683
LmnI GCTCC 1 cut(s) 734
Lsp1109I GCAGC 1 cut(s) 749
LweI GCATC 3 cut(s) 14, 244, 446
MaeI CTAG 2 cut(s) 59, 405
MaeII ACGT 2 cut(s) 10, 703
MaeIII GTNAC 2 cut(s) 593, 717
MalI GATC 4 cut(s) 246, 285, 327, 685
MboI GATC 4 cut(s) 244, 283, 325, 683
MboII GAAGA 3 cut(s) 257, 380, 649
MflI RGATCY 1 cut(s) 244
MhlI GDGCHC 2 cut(s) 358, 613
MlsI TGGCCA 2 cut(s) 50, 647
MluCI AATT 5 cut(s) 289, 305, 371, 556, 641
MluNI TGGCCA 2 cut(s) 50, 647
MmeI TCCRAC 1 cut(s) 661
MnlI CCTC 5 cut(s) 133, 331, 369, 448, 595
Mox20I TGGCCA 2 cut(s) 50, 647
MscI TGGCCA 2 cut(s) 50, 647
MslI CAYNNNNRTG 1 cut(s) 745
Msp20I TGGCCA 2 cut(s) 50, 647
MspI CCGG 1 cut(s) 743
MwoI GCNNNNNNNGC 2 cut(s) 353, 362
NdeI CATATG 1 cut(s) 770
NdeII GATC 4 cut(s) 244, 283, 325, 683
NlaIII CATG 3 cut(s) 318, 565, 694
PagI TCATGA 1 cut(s) 690
PfeI GAWTC 5 cut(s) 17, 159, 271, 415, 569
PinAI ACCGGT 1 cut(s) 742
PkrI GCNGC 1 cut(s) 739
Ppu21I YACGTR 2 cut(s) 11, 704
PstNI CAGNNNCTG 1 cut(s) 424
PsuI RGATCY 1 cut(s) 244
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
RseI CAYNNNNRTG 1 cut(s) 745
SatI GCNGC 1 cut(s) 738
Sau3AI GATC 4 cut(s) 244, 283, 325, 683
SduI GDGCHC 2 cut(s) 358, 613
SfaNI GCATC 3 cut(s) 14, 244, 446
SmiMI CAYNNNNRTG 1 cut(s) 745
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
SpeI ACTAGT 1 cut(s) 58
Sse9I AATT 5 cut(s) 289, 305, 371, 556, 641
SspI AATATT 1 cut(s) 123
SspMI CTAG 2 cut(s) 59, 405
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 434, 599
TaiI ACGT 2 cut(s) 13, 706
TaqI TCGA 1 cut(s) 590
TasI AATT 5 cut(s) 289, 305, 371, 556, 641
TfiI GAWTC 5 cut(s) 17, 159, 271, 415, 569
TscAI CASTG 3 cut(s) 78, 283, 437
TseI GCWGC 1 cut(s) 737
TspDTI ATGAA 4 cut(s) 30, 219, 318, 679
TspRI CASTG 3 cut(s) 78, 283, 437
VneI GTGCAC 1 cut(s) 354
XapI RAATTY 2 cut(s) 305, 556
XspI CTAG 2 cut(s) 59, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.