Rmu_sc0007069.1_g000004

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007069.1
OG0000800
Physical Location & Seq
Reverse (-)
12413 .. 28547
16135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007069.1_g000004.1.cds

Sequence Viewer

Length: 6072 bp
atggcctccgccaccgaaatctccgaaactctcctctccaagctcgaatccgccgattccgccgggattcatcgcctcatctccgactacctaaacccattcgccgaccttcacaaaaagaagacctcaaccgaccaccaaaccctacaacgctctctcgccaaaaaattcacccccttcctcaaccgcgccttagccatcctccccaaacggctcgccgatccttcaaagctagatgccaaatttgcccccgaattgttcgatatttaccgcctctgcctcgactccatggacgccgtctcctcccaattttccggcaagccctacaagtttcacatccagaggatccggttggtttatgcattcgaggcctggggccgccacaaggacgccgagtccgaggcgcttagggttttgaagagcttcaaggcgattgaactaggaacgaacccggtgaatttggaccgtagatttgtgccaggtgtcgagcagggaggctgcgataaggagtttggggctttgctcggagaagtggtgtggatgctagccaagtgtgcttcgctaattcaaagcaaggaagccgaggtgtatgagcggctgctttgtatggttgaggagttgggaccttggtttagggtgttagatgaaactgtacatgacaaggtgcacagacagcttgccatttatctcgagaggtgtactctttttttagtccgggagctatcatccttgggagcaaatctcgtattcaagttctgccgcataacagtgagcgagtatgcaaagtcatccatcaaagatcatatgttcaagtgcgcccgtcgaatatgttcagttgtattttcattacaagagactagttcctcattgatcagtaacacattgttatgcttgtttgactcccttgttgatgaatgcaaggctgaggtcgaatattcaggaaaagattttattgaacttattgcttattgtgccaataagtgtcgaactgcaagtacaaatctttctggtactatgagatctcattcaaaaaaattaataactcactttgatcaggatccacctttaaatttgattctaagacttcatgctactggtttgcactacgttgatcgtagtataaagtcaagggatgaggatcttacatcttctggaggtgcaattagaatgttgcttgatgatgaggacacaatgactgatttgtcagattgtcttgcttcattagaaagttatttccaaattggtacaaatgatatttccaaaagtgggttttccacgagtcacgtgcagaaggatttaaaggattatcttctgctgtattttaatgcattggagttcttatgccatccacttgctgacttagtatattcgggaaagaaacagatttttgctgataatggagctgcctctgtttctagtgaactgtgtgttatccagggtgcatttcatcaaattacttgtgagatagatatagatggatttaagcacaacagcatccttcgtgcttctgtggctgcttttatactttccaccagaacgaagcttaacatgcagaagagtgcacaaatactgaaccatgtcattaccagcgaatggattcaacttgatggtctaaagcatatatatgtctctctctacaatactggtacatgtttgtacaaaaacaaggagctgaatgaggcttcagaggctttgaagctttcctgcaaggcatcttggactcgtgttatacatctctgtgaactgtttgcaggcaaattaaaggccgccagtgctgatccttctgaagatactattttagatgtcttgaatgagtcgtgtacaggaagtgcttttctattggatattcttaaccaagttgataatcatgaggtgaaaagagctatggcagagtgccttgaaacttggtctattgcagcaaatttgtttgggaggctgccaggtccaatgtctgtggtgaagcagtgggttaagggacttcttgcttacgagaaaatgtatgctgtgaaccccgagttttgtcagaagatgcaaatgcaaattataaacatactactgcaagatgtgtatgtgacaccagacagttggttacaaaaagcaagaattttagtgagaaagggaaaagcgttaaaactttacgggatcaaaggtctaaaagactgtattcattgtttatcagaagcaatatcttcacttagtaagatgtacgataacaccagtatccatcaaacatctcctcgccatgaattagctgtggcatattgcttacgcgcgctttgtacccaggaggatgaaccacaatcagagaaaatgtttcaagatatccgtgctgcccttgatgcatggttggacatttctactacagacatgtgctctccagatgacaattgctccccagatgacaaatgtttaatggtgtctgaaaatacaataatgctcctatacaatattattgatttgttgtctatcaagaaaatgtttcaagatatccgtgctgcccttgatgcatggttgggcatttctactacagacatgtgctctccagatgacaattgctctccagatgacaaatgtttaatggtgtctgaaaatacaatgatgctcctatacaatattattgatttgttgtctatcaagggtcacatggattattaccatgatatatataggcttttgattagattatttaactggaaaaatatcccgttagagaagtgcttggtcagattgtgggagtgcaggagagttagtcatgccctttgtacttcacctgttaatgatgcactcatcatgagcttatcagatcattttggtgaacttccaaagtctattgatttttggatagattgtcttaaaggatcccaacaattggttcctgtatcaagtcagtcggcttatgtagctgcatatctctactatgatttatgtgaaagactagtttcaaatggacaactagttgaggctctatcatatgcaatggaagctcaccagttacgccttgaactattccaaaagaagtttgcgtactcttttgtacgaaaggctgaaaagtacaatgaaactagggatacctatcagaactttacactcgaagatcataatctggaagttaaacgatcagttgtccgtgaagtttggtcctttgacactagttcatgtaacttggaaaactgttatcttagtccctataatgtacttcaatgttatcttgaaagtactcttcaggtagggattatccatgaaatagttggaaatggagctgaggctgaggtagttttacagtggggaaagactatttcctgctcacaaagcttgccactatttgcggttgcattttgttcagtcttaggaaaactgtatcataagaagcagctttgggatttggcagagaaggaacttcaaagtgccaaccaatatttgaaggctagtgcaaaagatatttcttgcgtaaagtgcagattgatgcagaaagcaactgttaaactgaatcttggtgatttataccaaagcaaattttatagttcaaggagtgcatctttagagaaatcgtcttatgctgaaagtttgtacaaatcagcacttaccaagctaaacctttctgagtggcaaaattctgttagttgccctggacaaactgtacttaaagaagttggatattctgctggcagtatttctagtcattttgcggaaaccaaggtggaaccaaaaagcaagatagaagctaaaaagtctaggactaagaatgcccgaaaaccagtggggagggatcaaggttcagttcctgagtataatttgaggtcaactcagtctagatgccaatcttctcagaaccaaagtgtaagaggtactggtgtccgagttggtcatccaaaacatttgaaacgaagcactgagtgtgattcttctgataatttaagtaacaaggactttcttttggatttgaaaacttgcgaggtttcatatgggtctgacctaacatgcatttgcaacaaaatgaggtgttggcattgtcttcccatggaagttatggaatctgggctactgaaaaactttgtagatttgaaatgggagtatgtccgcaggcgtctctctctcaggcttctgacaggattaggaaattgtttggagaatcaaggtcaaattcatgaaacaaatgaatttatcctgcaaagcgtatcgattttagtcagcaggaacccattttgtataactacctcatcgattccgtcgacttccttgcttgatttaatggcaaaggaaatccctggggatgtgcttagtgttgagcgtgcggaagtactgtacagcatatgttggctgtctttaaagattcgttccaagaataccaggttaatgcaatggcatctttatttcagggggcacatggtgctggctcatgcttgggtcgctccagagtcaattcaagaccttgaagaatttgtgacagaattcttttcaggccttccatgcaccacaatcatctgtataagtgtgcttggaggcccttatgctagtttgttacaagaattgttgtgttttccttcacgcatgcatgcatggattctggtttcacgcttgaactctaagaatcaacctatcagtatgcttctgccagtggattcagtattagaaggagattcagatgatgttagttatactggaataaaggattggcactgtccatggggttccactgtggttgatgatgtagctccagagtttagattgatattggaggggactcattcgtcatctgtgaaacatcctgtacaagatacaaatgagaaaaagcttaactggtgggtgcagaggaagaattttgatcgccgccttggtaaatttctgaagaacttggaagattcatggtttggtacttggaaacttgtcctactgggtgaatggtcaaactgcacgcagttggacttggtacataagaagctggtgcgcaatctgaaatccaaatgcaaaatggagatagatgagagtcttttgaaagtcatccttgggggttccaaatatgccctcgaaggaggtgcacgtgttacacagctttgtcataagaaaggttcctacgttagtaaagttggatatttagaggaagagaagtgtatggcatcatgtaatgattctaatggtggcgagaacttgtctgagttggcctccaaactagttttagaagcagtgaatgagcttgaaggtttacattcttctttaaatatagagccaacaatattagttgtggactacgaggtgcagatgcttccttgggagaatttaccaatcctaagaaaccaggaggtttatcgcatgccctctgttgggagcatttcggcaacagccaatagaaactaccacaatcaagatcaagttcagagtattgtgacttcatttcctttgatagatcccttggatgccttttatcttttgaatcccagtggtgatctaaattatacacaaaatgaatttgagacatggttcagagatcaaaacttggaggggaaagccggatgtgcacctcctgctgaagaattggcagtagcgttgaaaagccatgatctatttatgtattttggccatggatgtggtttcctaataattcgttttgaactttatttgtgtacatgctgtggtacagggcaacaatatattcctagacgtgagattcagagactggaacactgtgccgcaactctccttatgggatgcagtagtggttctctgaaactgaatggatgttatattccacaaggtactccactatcctatctgctggccggttctcctgtcattgtcgccaatttgtgggatgtgacggacagggatattgaccgatttgcaaagtccatgcttgattcttggttgaaagcaaggtcgagtccctgtgtgagttgtgcccaatgcaacaagaaagtcacaaaaaagaaattggccacaaacctgtcttgtgagcataggccgaaggttggatcattcatgagcgaagcacgtaaaacttgccagttacctttcttgattggagcagcaccagtatgctatggtgtcccaactggcatatggaaaaaggagttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

2023

Amino Acids

228.12

Weight (kDa)

6.57

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2045
AasI GACNNNNNNGTC 2 cut(s) 1201, 4747
Acc16I TGCGCA 1 cut(s) 4946
AccB7I CCANNNNNTGG 3 cut(s) 1593, 2869, 5802
AccBSI CCGCTC 1 cut(s) 595
AccI GTMKAC 1 cut(s) 4229
AccII CGCG 3 cut(s) 189, 2271, 2273
AcoI YGGCCR 3 cut(s) 5572, 5772, 5928
AcuI CTGAAG 5 cut(s) 1668, 1806, 3206, 4865, 5544
AcvI CACGTG 2 cut(s) 1285, 5039
AcyI GRCGYC 3 cut(s) 294, 390, 4084
AdeI CACNNNGTG 2 cut(s) 3894, 4387
AfiI CCNNNNNNNGG 8 cut(s) 385, 1266, 1593, 2869, 5318, 5319, 5802, 5963
AflIII ACRYGT 4 cut(s) 1649, 2367, 2532, 5038
AhdI GACNNNNNGTC 1 cut(s) 394
AhlI ACTAGT 5 cut(s) 857, 2935, 2953, 3149, 5167
AjiI CACGTC 1 cut(s) 5657
AjnI CCWGG 9 cut(s) 371, 478, 1434, 1939, 2283, 3625, 4264, 4346, 5292
AjuI GAANNNNNNNTTGG 4 cut(s) 2856, 2888, 4654, 4686
Alw21I GWGCWC 6 cut(s) 669, 1564, 2375, 2540, 5038, 5515
Alw26I GTCTC 6 cut(s) 304, 848, 1633, 4091, 5462, 5662
Alw44I GTGCAC 4 cut(s) 665, 1560, 5034, 5511
AlwNI CAGNNNCTG 2 cut(s) 3782, 5671
Ama87I CYCGRG 2 cut(s) 689, 2012
ApaLI GTGCAC 4 cut(s) 665, 1560, 5034, 5511
ArsI GACNNNNNNTTYG 2 cut(s) 455, 487
AseI ATTAAT 1 cut(s) 1037
Asp700I GAANNNNTTC 3 cut(s) 422, 829, 1596
AspLEI GCGC 6 cut(s) 191, 406, 818, 2273, 2275, 4947
AspS9I GGNCC 6 cut(s) 376, 463, 623, 1943, 3138, 4502
AsuC2I CCSGG 3 cut(s) 64, 452, 716
AsuNHI GCTAGC 1 cut(s) 544
AvaI CYCGRG 2 cut(s) 689, 2012
AvaII GGWCC 4 cut(s) 463, 623, 1943, 3138
BaeGI GKGCMC 6 cut(s) 669, 1564, 4383, 5038, 5515, 5896
BaeI ACNNNNGTAYC 4 cut(s) 2203, 2236, 3837, 3870
BalI TGGCCA 2 cut(s) 5574, 5930
BamHI GGATCC 3 cut(s) 345, 1057, 2858
BarI GAAGNNNNNNTAC 4 cut(s) 3008, 3040, 5368, 5400
BauI CACGAG 2 cut(s) 1276, 1722
BbrPI CACGTG 2 cut(s) 1285, 5039
BbsI GAAGAC 2 cut(s) 128, 4004
Bbv12I GWGCWC 6 cut(s) 669, 1564, 2375, 2540, 5038, 5515
BbvCI CCTCAGC 3 cut(s) 924, 3261, 3267
BccI CCATC 6 cut(s) 206, 800, 1353, 1469, 1601, 2232
BceAI ACGGC 2 cut(s) 227, 281
BcgI CGANNNNNNTGC 6 cut(s) 1267, 1301, 4219, 4253, 5015, 5049
BciT130I CCWGG 9 cut(s) 373, 480, 1436, 1941, 2285, 3627, 4266, 4348, 5294
BciVI GTATCC 2 cut(s) 2231, 3061
BclI TGATCA 2 cut(s) 870, 1051
BcnI CCSGG 3 cut(s) 64, 452, 716
BcoDI GTCTC 6 cut(s) 304, 848, 1633, 4091, 5462, 5662
BcuI ACTAGT 5 cut(s) 857, 2935, 2953, 3149, 5167
BfmI CTRYAG 2 cut(s) 2361, 2526
BfoI RGCGCY 1 cut(s) 407
BfuI GTATCC 2 cut(s) 2231, 3061
BglII AGATCT 1 cut(s) 1019
BmcAI AGTACT 2 cut(s) 3217, 4299
Bme18I GGWCC 4 cut(s) 463, 623, 1943, 3138
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 2 cut(s) 689, 2012
BmgBI CACGTC 1 cut(s) 5657
BmgT120I GGNCC 6 cut(s) 376, 463, 623, 1943, 3138, 4502
BmrI ACTGGG 2 cut(s) 4901, 5427
BmtI GCTAGC 1 cut(s) 548
BmuI ACTGGG 2 cut(s) 4901, 5427
BpiI GAAGAC 2 cut(s) 128, 4004
BplI GAGNNNNNCTC 8 cut(s) 685, 717, 726, 758, 3787, 3819, 5144, 5176
BpmI CTGGAG 6 cut(s) 1173, 2361, 2526, 2544, 4395, 4698
Bpu10I CCTNAGC 5 cut(s) 193, 407, 924, 3261, 3267
BpuMI CCSGG 3 cut(s) 64, 452, 716
Bsa29I ATCGAT 2 cut(s) 4178, 4220
BsaAI YACGTR 3 cut(s) 1285, 5039, 5987
BsaBI GATNNNNATC 5 cut(s) 1056, 1137, 3072, 3099, 3817
BsaHI GRCGYC 3 cut(s) 294, 390, 4084
BsaWI WCCGGW 1 cut(s) 348
BsaXI ACNNNNNCTCC 8 cut(s) 284, 314, 519, 549, 2375, 2405, 2734, 2764
Bsc4I CCNNNNNNNGG 8 cut(s) 385, 1266, 1593, 2869, 5318, 5319, 5802, 5963
Bse118I RCCGGY 1 cut(s) 5774
Bse3DI GCAATG 2 cut(s) 2982, 4364
Bse8I GATNNNNATC 5 cut(s) 1056, 1137, 3072, 3099, 3817
BseBI CCWGG 9 cut(s) 373, 480, 1436, 1941, 2285, 3627, 4266, 4348, 5294
BseCI ATCGAT 2 cut(s) 4178, 4220
BseJI GATNNNNATC 5 cut(s) 1056, 1137, 3072, 3099, 3817
BseLI CCNNNNNNNGG 8 cut(s) 385, 1266, 1593, 2869, 5318, 5319, 5802, 5963
BseMI GCAATG 2 cut(s) 2982, 4364
BsePI GCGCGC 1 cut(s) 2271
BseRI GAGGAG 4 cut(s) 23, 292, 629, 2226
BseSI GKGCMC 6 cut(s) 669, 1564, 4383, 5038, 5515, 5896
BsgI GTGCAG 6 cut(s) 1307, 2758, 3475, 4826, 4894, 5273
Bsh1236I CGCG 3 cut(s) 189, 2271, 2273
BshVI ATCGAT 2 cut(s) 4178, 4220
BsiHKAI GWGCWC 6 cut(s) 669, 1564, 2375, 2540, 5038, 5515
BsiHKCI CYCGRG 2 cut(s) 689, 2012
BsiSI CCGG 7 cut(s) 63, 315, 349, 452, 715, 5505, 5775
BslFI GGGAC 6 cut(s) 636, 1989, 3168, 4753, 5862, 6026
BslI CCNNNNNNNGG 8 cut(s) 385, 1266, 1593, 2869, 5318, 5319, 5802, 5963
BsmAI GTCTC 6 cut(s) 304, 848, 1633, 4091, 5462, 5662
BsmBI CGTCTC 2 cut(s) 304, 4091
BsmFI GGGAC 6 cut(s) 636, 1989, 3168, 4753, 5862, 6026
BsmI GAATGC 3 cut(s) 362, 920, 3748
BsoBI CYCGRG 2 cut(s) 689, 2012
Bsp1286I GDGCHC 8 cut(s) 669, 1564, 2375, 2540, 4383, 5038, 5515, 5896
Bsp1407I TGTACA 7 cut(s) 652, 1656, 1820, 3567, 4302, 4768, 5618
Bsp19I CCATGG 4 cut(s) 288, 4017, 4682, 5575
BspDI ATCGAT 2 cut(s) 4178, 4220
BspFNI CGCG 3 cut(s) 189, 2271, 2273
BspHI TCATGA 4 cut(s) 1867, 2790, 4144, 5973
BspOI GCTAGC 1 cut(s) 548
BspQI GCTCTTC 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 595
BsrDI GCAATG 2 cut(s) 2982, 4364
BsrFI RCCGGY 1 cut(s) 5774
BsrGI TGTACA 7 cut(s) 652, 1656, 1820, 3567, 4302, 4768, 5618
BssAI RCCGGY 1 cut(s) 5774
BssHII GCGCGC 1 cut(s) 2271
BssNI GRCGYC 3 cut(s) 294, 390, 4084
BssSI CACGAG 2 cut(s) 1276, 1722
Bst2BI CACGAG 2 cut(s) 1276, 1722
Bst2UI CCWGG 9 cut(s) 373, 480, 1436, 1941, 2285, 3627, 4266, 4348, 5294
Bst6I CTCTTC 4 cut(s) 413, 1550, 3225, 5094
BstACI GRCGYC 3 cut(s) 294, 390, 4084
BstAPI GCANNNNNTGC 2 cut(s) 4387, 5519
BstAUI TGTACA 7 cut(s) 652, 1656, 1820, 3567, 4302, 4768, 5618
BstBAI YACGTR 3 cut(s) 1285, 5039, 5987
BstDSI CCRYGG 4 cut(s) 288, 4017, 4682, 5575
BstFNI CGCG 3 cut(s) 189, 2271, 2273
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 6 cut(s) 191, 406, 818, 2273, 2275, 4947
BstMAI GTCTC 6 cut(s) 304, 848, 1633, 4091, 5462, 5662
BstNI CCWGG 9 cut(s) 373, 480, 1436, 1941, 2285, 3627, 4266, 4348, 5294
BstNSI RCATGY 9 cut(s) 1552, 1653, 2371, 2536, 3981, 4552, 4556, 5311, 5625
BstSFI CTRYAG 2 cut(s) 2361, 2526
BstSLI GKGCMC 6 cut(s) 669, 1564, 4383, 5038, 5515, 5896
BstUI CGCG 3 cut(s) 189, 2271, 2273
BstV2I GAAGAC 2 cut(s) 128, 4004
BstX2I RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2858, 5401
BstXI CCANNNNNNTGG 2 cut(s) 2085, 5582
BstYI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2858, 5401
Bsu15I ATCGAT 2 cut(s) 4178, 4220
BsuI GTATCC 2 cut(s) 2231, 3061
BsuTUI ATCGAT 2 cut(s) 4178, 4220
BtgI CCRYGG 4 cut(s) 288, 4017, 4682, 5575
BtgZI GCGATG 1 cut(s) 56
BtrI CACGTC 1 cut(s) 5657
BtsI GCAGTG 2 cut(s) 1970, 5187
CaiI CAGNNNCTG 2 cut(s) 3782, 5671
CciI TCATGA 4 cut(s) 1867, 2790, 4144, 5973
CfoI GCGC 6 cut(s) 191, 406, 818, 2273, 2275, 4947
Cfr10I RCCGGY 1 cut(s) 5774
Cfr13I GGNCC 6 cut(s) 376, 463, 623, 1943, 3138, 4502
ClaI ATCGAT 2 cut(s) 4178, 4220
CseI GACGC 3 cut(s) 302, 398, 4073
CsiI ACCWGGT 1 cut(s) 4346
CspCI CAANNNNNGTGG 4 cut(s) 1935, 1970, 4681, 4716
DraI TTTAAA 4 cut(s) 1068, 1299, 4326, 5214
DraIII CACNNNGTG 2 cut(s) 3894, 4387
DrdI GACNNNNNNGTC 2 cut(s) 1201, 4747
DriI GACNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 2 cut(s) 1201, 4747
EaeI YGGCCR 3 cut(s) 5572, 5772, 5928
Eam1104I CTCTTC 4 cut(s) 413, 1550, 3225, 5094
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 4 cut(s) 413, 1550, 3225, 5094
EciI GGCGGA 2 cut(s) 40, 49
Eco147I AGGCCT 2 cut(s) 371, 4461
Eco32I GATATC 2 cut(s) 2323, 2488
Eco47I GGWCC 4 cut(s) 463, 623, 1943, 3138
Eco57I CTGAAG 5 cut(s) 1668, 1806, 3206, 4865, 5544
Eco72I CACGTG 2 cut(s) 1285, 5039
Eco88I CYCGRG 2 cut(s) 689, 2012
EcoO109I RGGNCCY 2 cut(s) 623, 4502
EcoRI GAATTC 1 cut(s) 4448
EcoRII CCWGG 9 cut(s) 371, 478, 1434, 1939, 2283, 3625, 4264, 4346, 5292
EcoRV GATATC 2 cut(s) 2323, 2488
EcoT22I ATGCAT 7 cut(s) 364, 1330, 2344, 2509, 3983, 4554, 4558
Esp3I CGTCTC 2 cut(s) 304, 4091
FalI AAGNNNNNCTT 2 cut(s) 4986, 5018
FaqI GGGAC 6 cut(s) 636, 1989, 3168, 4753, 5862, 6026
FauNDI CATATG 5 cut(s) 804, 2971, 3961, 4310, 6053
FbaI TGATCA 2 cut(s) 870, 1051
FblI GTMKAC 1 cut(s) 4229
FspI TGCGCA 1 cut(s) 4946
GlaI GCGC 6 cut(s) 190, 405, 817, 2272, 2274, 4946
GsuI CTGGAG 6 cut(s) 1173, 2361, 2526, 2544, 4395, 4698
HaeII RGCGCY 1 cut(s) 407
HapII CCGG 7 cut(s) 63, 315, 349, 452, 715, 5505, 5775
HgaI GACGC 3 cut(s) 302, 398, 4073
HhaI GCGC 6 cut(s) 191, 406, 818, 2273, 2275, 4947
Hin1I GRCGYC 3 cut(s) 294, 390, 4084
Hin6I GCGC 6 cut(s) 189, 404, 816, 2271, 2273, 4945
HinP1I GCGC 6 cut(s) 189, 404, 816, 2271, 2273, 4945
HincII GTYRAC 2 cut(s) 3801, 4230
HindII GTYRAC 2 cut(s) 3801, 4230
HindIII AAGCTT 4 cut(s) 1541, 1697, 3310, 4790
HpaII CCGG 7 cut(s) 63, 315, 349, 452, 715, 5505, 5775
Hpy99I CGWCG 2 cut(s) 825, 4231
HpyCH4IV ACGT 6 cut(s) 1107, 1284, 5038, 5073, 5656, 5986
HpySE526I ACGT 6 cut(s) 1107, 1284, 5038, 5073, 5656, 5986
Hsp92I GRCGYC 3 cut(s) 294, 390, 4084
HspAI GCGC 6 cut(s) 189, 404, 816, 2271, 2273, 4945
Ksp22I TGATCA 2 cut(s) 870, 1051
LguI GCTCTTC 1 cut(s) 413
MabI ACCWGGT 1 cut(s) 4346
MaeII ACGT 6 cut(s) 1107, 1284, 5038, 5073, 5656, 5986
MbiI CCGCTC 1 cut(s) 595
MfeI CAATTG 3 cut(s) 2386, 2551, 2867
MflI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2858, 5401
MhlI GDGCHC 8 cut(s) 669, 1564, 2375, 2540, 4383, 5038, 5515, 5896
MlsI TGGCCA 2 cut(s) 5574, 5930
MluNI TGGCCA 2 cut(s) 5574, 5930
MmeI TCCRAC 7 cut(s) 108, 2328, 3229, 3631, 4899, 5065, 5944
Mox20I TGGCCA 2 cut(s) 5574, 5930
Mph1103I ATGCAT 7 cut(s) 364, 1330, 2344, 2509, 3983, 4554, 4558
MroXI GAANNNNTTC 3 cut(s) 422, 829, 1596
MscI TGGCCA 2 cut(s) 5574, 5930
MslI CAYNNNNRTG 7 cut(s) 767, 886, 1623, 1737, 2811, 5580, 6028
Msp20I TGGCCA 2 cut(s) 5574, 5930
MspI CCGG 7 cut(s) 63, 315, 349, 452, 715, 5505, 5775
MunI CAATTG 3 cut(s) 2386, 2551, 2867
Mva1269I GAATGC 3 cut(s) 362, 920, 3748
MvaI CCWGG 9 cut(s) 373, 480, 1436, 1941, 2285, 3627, 4266, 4348, 5294
MvnI CGCG 3 cut(s) 189, 2271, 2273
NciI CCSGG 3 cut(s) 64, 452, 716
NcoI CCATGG 4 cut(s) 288, 4017, 4682, 5575
NdeI CATATG 5 cut(s) 804, 2971, 3961, 4310, 6053
NheI GCTAGC 1 cut(s) 544
NmeAIII GCCGAG 2 cut(s) 418, 607
NmuCI GTSAC 7 cut(s) 1280, 2071, 2639, 4441, 5380, 5809, 5911
NsbI TGCGCA 1 cut(s) 4946
NsiI ATGCAT 7 cut(s) 364, 1330, 2344, 2509, 3983, 4554, 4558
NspI RCATGY 9 cut(s) 1552, 1653, 2371, 2536, 3981, 4552, 4556, 5311, 5625
PaeI GCATGC 3 cut(s) 4552, 4556, 5311
PaeR7I CTCGAG 1 cut(s) 689
PagI TCATGA 4 cut(s) 1867, 2790, 4144, 5973
PasI CCCWGGG 1 cut(s) 4265
PauI GCGCGC 1 cut(s) 2271
PceI AGGCCT 2 cut(s) 371, 4461
PciI ACATGT 3 cut(s) 1649, 2367, 2532
PciSI GCTCTTC 1 cut(s) 413
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 396, 4226
PctI GAATGC 3 cut(s) 362, 920, 3748
PdmI GAANNNNTTC 3 cut(s) 422, 829, 1596
PflFI GACNNNGTC 1 cut(s) 296
PflMI CCANNNNNTGG 3 cut(s) 1593, 2869, 5802
PfoI TCCNGGA 1 cut(s) 714
PmaCI CACGTG 2 cut(s) 1285, 5039
PmlI CACGTG 2 cut(s) 1285, 5039
Ppu21I YACGTR 3 cut(s) 1285, 5039, 5987
PpuMI RGGWCCY 1 cut(s) 623
PscI ACATGT 3 cut(s) 1649, 2367, 2532
PshBI ATTAAT 1 cut(s) 1037
PsiI TTATAA 1 cut(s) 2045
Psp5II RGGWCCY 1 cut(s) 623
Psp6I CCWGG 9 cut(s) 371, 478, 1434, 1939, 2283, 3625, 4264, 4346, 5292
PspCI CACGTG 2 cut(s) 1285, 5039
PspGI CCWGG 9 cut(s) 371, 478, 1434, 1939, 2283, 3625, 4264, 4346, 5292
PspPI GGNCC 6 cut(s) 376, 463, 623, 1943, 3138, 4502
PspPPI RGGWCCY 1 cut(s) 623
PsrI GAACNNNNNNTAC 2 cut(s) 979, 1011
PstNI CAGNNNCTG 2 cut(s) 3782, 5671
PsuI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2858, 5401
PsyI GACNNNGTC 1 cut(s) 296
PteI GCGCGC 1 cut(s) 2271
RseI CAYNNNNRTG 7 cut(s) 767, 886, 1623, 1737, 2811, 5580, 6028
SalI GTCGAC 1 cut(s) 4228
SapI GCTCTTC 1 cut(s) 413
Sau96I GGNCC 6 cut(s) 376, 463, 623, 1943, 3138, 4502
ScaI AGTACT 2 cut(s) 3217, 4299
SduI GDGCHC 8 cut(s) 669, 1564, 2375, 2540, 4383, 5038, 5515, 5896
SexAI ACCWGGT 1 cut(s) 4346
SfcI CTRYAG 2 cut(s) 2361, 2526
Sfr274I CTCGAG 1 cut(s) 689
SinI GGWCC 4 cut(s) 463, 623, 1943, 3138
SlaI CTCGAG 1 cut(s) 689
SmiMI CAYNNNNRTG 7 cut(s) 767, 886, 1623, 1737, 2811, 5580, 6028
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
SpeI ACTAGT 5 cut(s) 857, 2935, 2953, 3149, 5167
SphI GCATGC 3 cut(s) 4552, 4556, 5311
SseBI AGGCCT 2 cut(s) 371, 4461
SspI AATATT 5 cut(s) 935, 2449, 2614, 3416, 5232
StuI AGGCCT 2 cut(s) 371, 4461
TaiI ACGT 6 cut(s) 1110, 1287, 5041, 5076, 5659, 5989
TaqII GACCGA 1 cut(s) 5844
TauI GCSGC 6 cut(s) 381, 598, 762, 1769, 4830, 5687
TseFI GTSAC 7 cut(s) 1280, 2071, 2639, 4441, 5380, 5809, 5911
Tsp45I GTSAC 7 cut(s) 1280, 2071, 2639, 4441, 5380, 5809, 5911
TspGWI ACGGA 5 cut(s) 2315, 2480, 3116, 4215, 5828
Tth111I GACNNNGTC 1 cut(s) 296
Van91I CCANNNNNTGG 3 cut(s) 1593, 2869, 5802
VneI GTGCAC 4 cut(s) 665, 1560, 5034, 5511
VpaK11BI GGWCC 4 cut(s) 463, 623, 1943, 3138
VspI ATTAAT 1 cut(s) 1037
XbaI TCTAGA 1 cut(s) 3809
XceI RCATGY 9 cut(s) 1552, 1653, 2371, 2536, 3981, 4552, 4556, 5311, 5625
XcmI CCANNNNNNNNNTGG 4 cut(s) 3245, 4024, 4966, 6051
XhoI CTCGAG 1 cut(s) 689
XmiI GTMKAC 1 cut(s) 4229
XmnI GAANNNNTTC 3 cut(s) 422, 829, 1596
ZrmI AGTACT 2 cut(s) 3217, 4299
Zsp2I ATGCAT 7 cut(s) 364, 1330, 2344, 2509, 3983, 4554, 4558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.