RLG00000005491

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000800
Physical Location & Seq
Reverse (-)
68060673 .. 68074939
14267 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005491

Sequence Viewer

Length: 5892 bp
ATGACCTCCTCCGAAGCCGACGTTCTGATTTCCGACCTCTCCACAGGTAAAGCAGGGATTCATGGACGCATTTCGGCCTACCTACGTGTGTTCAAAGAACCCCTCAGCACCCCAGCCCAAAAGCAATCTCTTATCACCGATTACCACCCCTTCCTCAACCGAGCGCTTACTCTTCTCCATGGAAAGCTAGCTCATCCTTCAAGGTTGAATGAGAAATTCGCCGCCGAATGGTTCGCTGTTTACAGGCTCTGCGTCAGCTGCATGGCCGTCGTCTCCGGGGTTCGAATGAAACCACTCCTGCTCCTTCATGAAAGGTACAAGATCCTGTGGTGTTTCGCTCGTTGGCGTCGTTGGGAGGATGGCGAGAGGGAGGGACTAGCAATTTTGAAGGGAATCAAGGAGATCCAACGCCTCCCTTCTGTCAGTGTCACATCCAACTTATATTTGCCGGATACCGATGATGCAGCATTGCTTGCCAAAACGGTGCCCTTGCTGTTTGTTTGCGCAGCAAATAATAAGCCCAAGCATCCTGACCGGTATCACCGCCTTTATCATATTGCTACCGAGTTTCATACATGGACCAGGGTATTGGATAAAGATCCCCGCCAGCTCGTGCTCGAGGAGCAAGCGAGCTCTCTTGTGACTTGTACCATCTTTTTAGTCGGCGAGGTTGATCAATTTGACTCCAAATTTGATCTTGCAAGCAAGTTTTGTGACCTAATTATCACCACATCAGTCCAAGGATCACCTCACCTAACGAAGCTATTGTCTCTCCAGGCTTCAGATGCTCTGAAGTTGTATTGTCAGGCAACATGGCTTATAATCGAAGGAAAACTAAAGGAGGCAGATCCTAGATTATCAAATTATGACAGCTTGGAATTATTTGGCAAGTGGTGTGAAGCAAGTACTTTACTACTCCAAGTTCTTAGTCAATGTGAGGGTACTCATGAAGTGCAAGGAGTTATAAAAGACTGGGCTGAAAAGTGGGCTGCTGAACATTTTTCTAATAGACTGCCAGGTCCCATCTCGTTGGTGGAGCAATGGGTGGAGGTAGCACCTGAGACTTTTCTAATTTTGTTTACTTACCCTGCTGTTTCTGTTTACTTACAATGCCGTGTCTTCTTACTTACCATGCGCACCTCATTTTTCTGTCAGATTCAGGTAAAACTCGTAAAAGGTCATGAAAATGGGGATGATGTGGGCACTTTGTTAGGCCATTTGTTTCTATCTTCTAGCAAAGTATCAAGAAAGAAGATCTTGCAGCAGGAACTTCTATTTTATGTTCAAATGCAAAATGACAACCCCAACTTTTGTAAGAGAATGCAAATGATGATTAAGGATTTACTAGAGAAAGATGGGTTTGGCACACTAATTGATTGGTTAGAGGAATCAAGCTGTTTAAGACTGTGTGGAGTAAGAGGTCCAAAATACTGCACTCAATGCTTGTCACAAGCACTTGAAGTTGCTTCTGGCCATCAATTAGCTGTGGCATATTGTTTACGATCACTTTGCACTGAGGAATATGAACCAAACTCAGAGAAAGTGTTTCAAGATATTCAATCTGCCCTTGCTGTGTGGTCGAGCATTCCTACTTCAGATGGCTCCATGGTGCCTGAAAATATAATGAAGATACTTTATTCTGTAATTGATTTGTTATCCCTCAAGGGTCGCGTAGACTTTCACTGTGCTATATACAAGGTTATGATTAAGATCTATACCCAGAGAGGAGTCTCGTTGAATAATTTGTTGGCCAAATTGTGGGAATCCAGAAGGATCAGTCATGCACTCTGCCCTTCTCCAGTTCAAGAGGCATTTATGAGCCTGTCAGATGTTTTTGGTGAAAAATTAAAGTTAACTGAGTTTTGGATAGAGTGTTTTAGTAAATCCCAGCCATTGGTAGTAGGGTTTCATAACAATCTATCATCTTCTCAATCCAATATAACAGTTCATAATGTTGAAGAAGTAACTTCTGCACTAATTTCAAGTGTTTCTGAATCTGGTTCTTACCTAGTTGGATATATGTACTACGATTTGTGTGAAAAATTGGTTTCAAATGGGCGTTCAATTGAGGCTCTTCCGTATGCAATGGAAGCCCACCGCTTACGAGCAGAACTATTCAAACAGAAATTTCCGAGGTCTTTTGAAGGACGCGGTGAAGGGAATCACGTGCTAGTCTGTAGATCAGTTGCTAGTGAACTATGGTCTTTTGATACTAATTCATGTGACTTGGATATCTGCTATATTAGCCCCTGGAATGCACTTCAGTGCTATCTTGAGAGCATTCTTCAGGTAGGGGTTATCCATGAAATACTGGGAAATGGAGCTCAGGCTGAATATTTTTTGGATCGGGGAAAAAGAGAACTATACCATAAGAAGCAGCGTTGGGCTTCAGCAAAAAAGGAACTTCAAAGTTCCAGGGAACTCTTGAAGGATGGCATAATAGATGGTTTTTGCTCAAAATGCAAAGTGATACTTGAAGTAACTGTTACACATAATCTTGCTGATTTATACCAAAGCCCCATCAGTGAAATTGAGAGTACATCTGCAGAGAAATTATCTACTGATCTCTACAAAGAAACTCTAGCCAAGCTACAAACTTCTGAGTGGAAAAAGTCTATTGCTTGTCTTGAAGAACAAGATGGTACTATTTTAGTCAAAGATGGTAATTCGCAACTTGAAGGCAGTATTGAGTGCGATTTTCCCAATGCATTGTGTGAGAAGGAATTTCCCTGGGATTTGGATTTGCAAAACTGTGTGGTATCATTTGGAGGTGATGTACCATGCATATGCAAAACAATGGGGTGTTGGCATTGTCTTACAGGGAAAGTAACAAAATCTGGCTTAGTGACTGACTGTATAAAGTTGAAGTGGGAACTAGTACACCGTCGTCTGTCACTCAAGCTGCTCACAGGATTAGGAAAATGCTTGGAGAATGATGGCCAAATTCATGAAGCATGTGAAATTATATTTCAAAGCATATCAGTATTAGTTGGACCAAACCCATTTTGTCGGGCAAGCAAATCAACTTTCTTGCCTGATACGATGGGAAAGGAAATCAGTGCTCAGCGGAAACATATAAACCTATATCATCTAATGGGAAGGGAAATCCCAGGAGATATGCTCAATGTTGAGCGTGCTGTAATACTTTACAACCTATGTTGGTTGTCTTTGAAGGACCGTAAGAAGGATACCAGCAATAATCCCCATGTGCTGATTGATATTCCTATATCAAAACTAGTGTCTTGGTTGATGCTTGCATTCGTCCTCTGCCGTGAGGTTCCTGCACTTTTTCAGAAGGTTTCGAGATTGCTGGCTGCTATTTTCTCACTGTCTACCTCAAGTGTGCTTGAAGCTCCATTGTCAACTTCTTGCAAAGATCTCTGTGGACGTCATTGGGTTTGCTTTTTTCATCAAGCTTCACTTGGAATTCAGTTTAATTACCAGTTTTCCATGAAAGAAGTCCAGCAGGGTGCAGACACTAAGGGTTCACACATGGCATCAGGAGCAGAGAGTTTACCTAGGCTCGCACCAGAATCTATTCTAAACCTTAAAGACTTTGTAAAAGAATTTTTTGAAGGCCTTCCTGGCACCACAGTCATCTGTCTAAGTTTACTTGGCTGCCCATATACTAGTATGTTTCAGGAATTATTGTCTTGTTCATCTGCTCCTGCATGGATTATGGTGTCTCGCTTAAACCCTAAGAATCTTCCTATACATATTCTTCTGCCGGTGCATTCAGTACCCGGAGGAGATTCAGATGGTGTTACAAATTCTGGTGAGAATGATGGTGGTCAGTGGCGCTGTCCATGGGGTTCCACTGTGGTTGATGATGTAGCTCCGAATTTTAGAATGCTATTGGAGGAGAATTTCAATTTAGCAAATTACGAGTTAGCTTCAAGTCCTGCTGAAAAGAAAGATCATATATATAATGTGTGGGAAGCGAGACGGAGGATTGATGATCGCCTTTGTACATTGCTGAAAAACTTGGAAGATTCATGGTTTGGTAGTACTTTGAAATATGTGCTTCTTGGTGAAGTGTCCAACTGCAAGGATCTGGACACGTTATGTGAGAACCTTGTCAGGAAATTGAAATCTCAATTCACGGACATAGATTCTAGTCTTCTGAAAGTCATCGTCCTTGGGGTGTCCAAATATGGCTTTAAAGGAGGCACAGAACAAGTTATGTTGGAGTTTTGCCTTAAGAAAGGTTGTTTCTTTAGTGATGTTCATTTCAAGGATGACCATTGCGCTGATGAATTGCGTGGCTTTGCTTCCAAACTACATGAAGCAGTGAAGAAGGTCAAAGGCTTTAATAGAATTATAAAGCCGATAATCTTAGTCCCAGACTTTGACGTCCAGATGCTTCCTTGGGAGAATTTACCGGTACTAAAGAACCAGGAGGTTTATCGCATGCCATCAGTTTGGAGTATATTGGCAGCATATAGAAATGAGCGCAAGATCAATCCTTTGGATGCCTATTATTTGTTCAACCCTGATGGTAGCCTGAATTGCAAACCCGATTTTGACAAATTGTTTAGAGATAATAAGTTGAAGGGGAAGGTTGGACATGCACCCCCAACTGAAAAACTGGCAGCAGCTTTGGAAAGCAGGGATCTCTTCATATATATAGGCCATGGAGATGGGTCGTTATATATTCCTAATGATAGGATCGAGAGACTCCGAAACTGTGCTGGGGCTTTCCTAATGGGATGCAGTAGTGCTTCTTTGAAACTAGATGGGTGTTATGTTCCACGAGGTCCTCCCCTATCGTATCTGCTTGCCGGTTCTCCCGTCATTTTCGGCAATTTATGGGAAGTAGCAGGCCTTGACATTGATAAATTTACACATGCTATGCTTGTCTCTTGGTTTAAAGATAGGTCTTACCGTAAAGATTGCGATCAGAGGCTAATGATTGGAACATGCATGAGTCGAGCCCGTGATAAGTGCAGTCATTTTCCTTGCTTGGTTGGGGCATCACCGATGATGTACATGAGTGTAGCATCTCAGATTGAATATGAGGCTATACATTTGGAGGATTTTGAGATCAAGCCGATGGATGAATCAGAATTAATCGAAGCATTAGAGTCAATGAAAAGGATAGATTTAACTGATGAAGAAATTTTGGAGATATGTATGGCGGATAAGCACGAAATGCACGATCATAAGAAATGTATGGAATTACAGGAAAAGGCTCTGAGCGGGGAGTATGCATCTCCGAATCCAGCTGATTCATACAACCTCTGGCAGCCAAGTGCTCATGAGGCCCGATTATGGTATAGGAAGCCCAACACGGACGGGCCAAGCCCGACGTACGGCCACCAAAGAAAGGCGTTTTACTCAATTCGAAGGTTTGGCCTAACTCTTGTAACAGTTACTACAGGAGCTTCTGTTGTGGAAAAGGATGATGTTTTGAAGGGGGGGTTTGGTGGGAGTAAAGCGTATGGTGATTTATCAAAATGTGGGAAGCCTGCTATGACTGAGGAGTCATCACAAAAGCAACAGCCTTCTGACATTGATGAAAGTAATATCCAAAGGAAAGGGGGGTTTGTTACAGAGGAAGATATAGCAGATGAAGATTCTGATTATGATTATGATTTTTTTGATGAACATATCGTGAAAAGGTCTGATCCAACTAAGGAAGAAATCTTGGAGATTATGGCGCATGAGCACGATCATAGGAGATGTATCGAATTACAGGAAAAGGCTTTGAAGGGGGAGTATGCATCTCAGAATCCAATTGATTCTCCACTTTTCAATTGGCCCTGGGAGGAGGAATTTGAGAGCAAGCCGATGGATGTATCAGAGTTGTTGGAAATATTAGATTCATTGAAAGAATTAGGAGAAGTTGATGGAACATATGAGTGTGTAGATGCAGCAACAGTTCTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

1964

Amino Acids

221.55

Weight (kDa)

5.74

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 8 - 248 4.4e-50 Separase-like, TPR repeats
Separin_C PF03568 1224 - 1611 8.9e-67 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 821, 965, 4322
AasI GACNNNNNNGTC 3 cut(s) 1017, 4352, 5482
AatII GACGTC 2 cut(s) 3391, 4356
Acc16I TGCGCA 2 cut(s) 505, 1136
AccB1I GGYRCC 3 cut(s) 484, 1609, 3587
AccB7I CCANNNNNTGG 2 cut(s) 1758, 1894
AccBSI CCGCTC 1 cut(s) 5199
AccI GTMKAC 2 cut(s) 1674, 3332
AccII CGCG 2 cut(s) 1671, 2151
AciI CCGC 8 cut(s) 222, 544, 604, 2098, 2151, 3067, 5138, 5199
AcoI YGGCCR 5 cut(s) 264, 1471, 1749, 2938, 5314
AcuI CTGAAG 6 cut(s) 765, 812, 1578, 2246, 2270, 2373
AcvI CACGTG 1 cut(s) 2167
AcyI GRCGYC 3 cut(s) 346, 3388, 4353
AfeI AGCGCT 1 cut(s) 165
AfiI CCNNNNNNNGG 7 cut(s) 228, 1758, 1894, 3184, 5271, 5312, 5326
AflII CTTAAG 1 cut(s) 4199
AflIII ACRYGT 2 cut(s) 85, 4059
AgeI ACCGGT 2 cut(s) 534, 4381
AhlI ACTAGT 3 cut(s) 2875, 3235, 3629
AjuI GAANNNNNNNTTGG 2 cut(s) 1730, 1762
AleI CACNNNNGTG 1 cut(s) 2263
Alw21I GWGCWC 6 cut(s) 618, 635, 2326, 3064, 5257, 5670
Alw26I GTCTC 8 cut(s) 277, 774, 1055, 1735, 3690, 3937, 4669, 4864
AlwNI CAGNNNCTG 1 cut(s) 249
Ama87I CYCGRG 1 cut(s) 617
Aor51HI AGCGCT 1 cut(s) 165
ArsI GACNNNNNNTTYG 6 cut(s) 674, 706, 752, 784, 3224, 3256
AseI ATTAAT 1 cut(s) 5069
AsiGI ACCGGT 2 cut(s) 534, 4381
Asp700I GAANNNNTTC 2 cut(s) 3537, 3579
AspA2I CCTAGG 1 cut(s) 3518
AspLEI GCGC 7 cut(s) 166, 506, 1137, 3801, 4250, 4455, 5662
AspS9I GGNCC 9 cut(s) 579, 1019, 1421, 2993, 3175, 4757, 5263, 5297, 5760
AsuC2I CCSGG 2 cut(s) 277, 3744
AsuII TTCGAA 2 cut(s) 283, 5344
AsuNHI GCTAGC 1 cut(s) 187
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 6 cut(s) 579, 1019, 1421, 2993, 3175, 4757
AvrII CCTAGG 1 cut(s) 3518
BaeGI GKGCMC 2 cut(s) 489, 1205
BalI TGGCCA 3 cut(s) 1473, 1751, 2940
BanI GGYRCC 3 cut(s) 484, 1609, 3587
BanII GRGCYC 3 cut(s) 635, 2326, 4936
BauI CACGAG 2 cut(s) 611, 4752
BbrPI CACGTG 1 cut(s) 2167
BbsI GAAGAC 2 cut(s) 1111, 4112
Bbv12I GWGCWC 6 cut(s) 618, 635, 2326, 3064, 5257, 5670
BbvCI CCTCAGC 1 cut(s) 104
BceAI ACGGC 4 cut(s) 251, 1098, 3255, 5329
BcgI CGANNNNNNTGC 4 cut(s) 250, 284, 1491, 1525
BciVI GTATCC 2 cut(s) 445, 3181
BclI TGATCA 1 cut(s) 673
BcnI CCSGG 2 cut(s) 277, 3744
BcoDI GTCTC 8 cut(s) 277, 774, 1055, 1735, 3690, 3937, 4669, 4864
BcuI ACTAGT 3 cut(s) 2875, 3235, 3629
BfmI CTRYAG 3 cut(s) 2176, 2544, 5376
BfoI RGCGCY 2 cut(s) 167, 3802
BfrI CTTAAG 1 cut(s) 4199
BfuI GTATCC 2 cut(s) 445, 3181
BglI GCCNNNNNGGC 1 cut(s) 3585
BglII AGATCT 3 cut(s) 1254, 1710, 3376
BlnI CCTAGG 1 cut(s) 3518
BlpI GCTNAGC 1 cut(s) 3063
BmcAI AGTACT 2 cut(s) 907, 4009
Bme18I GGWCC 6 cut(s) 579, 1019, 1421, 2993, 3175, 4757
BmeT110I CYCGRG 1 cut(s) 617
BmgT120I GGNCC 9 cut(s) 579, 1019, 1421, 2993, 3175, 4757, 5263, 5297, 5760
BmiI GGNNCC 7 cut(s) 486, 1021, 1603, 1611, 3279, 3589, 3814
BmrI ACTGGG 2 cut(s) 982, 2321
BmtI GCTAGC 1 cut(s) 191
BmuI ACTGGG 2 cut(s) 982, 2321
BpiI GAAGAC 2 cut(s) 1111, 4112
BplI GAGNNNNNCTC 2 cut(s) 3105, 3137
BpmI CTGGAG 2 cut(s) 758, 1782
Bpu10I CCTNAGC 2 cut(s) 104, 2325
Bpu1102I GCTNAGC 1 cut(s) 3063
Bpu14I TTCGAA 2 cut(s) 283, 5344
BpuEI CTTGAG 4 cut(s) 1646, 2294, 2882, 3322
BpuMI CCSGG 2 cut(s) 277, 3744
BsaAI YACGTR 2 cut(s) 86, 2167
BsaBI GATNNNNATC 3 cut(s) 597, 1709, 4896
BsaHI GRCGYC 3 cut(s) 346, 3388, 4353
BsaWI WCCGGW 2 cut(s) 534, 4381
BsaXI ACNNNNNCTCC 6 cut(s) 285, 315, 2760, 2790, 5373, 5403
Bsc4I CCNNNNNNNGG 7 cut(s) 228, 1758, 1894, 3184, 5271, 5312, 5326
Bse118I RCCGGY 4 cut(s) 534, 3727, 4381, 4781
Bse1I ACTGG 5 cut(s) 977, 1799, 2316, 3442, 4593
Bse3DI GCAATG 5 cut(s) 467, 1046, 2091, 3971, 4243
Bse8I GATNNNNATC 3 cut(s) 597, 1709, 4896
BseJI GATNNNNATC 3 cut(s) 597, 1709, 4896
BseLI CCNNNNNNNGG 7 cut(s) 228, 1758, 1894, 3184, 5271, 5312, 5326
BseMI GCAATG 5 cut(s) 467, 1046, 2091, 3971, 4243
BseNI ACTGG 5 cut(s) 977, 1799, 2316, 3442, 4593
BseRI GAGGAG 6 cut(s) 635, 1740, 3762, 3875, 5495, 5783
BseSI GKGCMC 2 cut(s) 489, 1205
BseYI CCCAGC 3 cut(s) 112, 1887, 4691
BsgI GTGCAG 5 cut(s) 1417, 1956, 3267, 3492, 4966
Bsh1236I CGCG 2 cut(s) 1671, 2151
BshNI GGYRCC 3 cut(s) 484, 1609, 3587
BshTI ACCGGT 2 cut(s) 534, 4381
BsiHKAI GWGCWC 6 cut(s) 618, 635, 2326, 3064, 5257, 5670
BsiHKCI CYCGRG 1 cut(s) 617
BsiSI CCGG 7 cut(s) 276, 449, 535, 3728, 3744, 4382, 4782
BsiWI CGTACG 1 cut(s) 5310
BslFI GGGAC 3 cut(s) 387, 1005, 4325
BslI CCNNNNNNNGG 7 cut(s) 228, 1758, 1894, 3184, 5271, 5312, 5326
BsmAI GTCTC 8 cut(s) 277, 774, 1055, 1735, 3690, 3937, 4669, 4864
BsmBI CGTCTC 2 cut(s) 277, 3937
BsmFI GGGAC 3 cut(s) 387, 1005, 4325
BsmI GAATGC 7 cut(s) 1326, 1584, 2260, 2280, 3257, 3733, 3855
BsoBI CYCGRG 1 cut(s) 617
Bsp119I TTCGAA 2 cut(s) 283, 5344
Bsp1286I GDGCHC 9 cut(s) 489, 618, 635, 1205, 2326, 3064, 4936, 5257, 5670
Bsp1407I TGTACA 2 cut(s) 3968, 4986
Bsp1720I GCTNAGC 1 cut(s) 3063
Bsp19I CCATGG 4 cut(s) 178, 1605, 3806, 4633
BspACI CCGC 8 cut(s) 222, 544, 604, 2098, 2151, 3067, 5138, 5199
BspFNI CGCG 2 cut(s) 1671, 2151
BspHI TCATGA 5 cut(s) 307, 946, 1180, 2947, 5257
BspLI GGNNCC 7 cut(s) 486, 1021, 1603, 1611, 3279, 3589, 3814
BspMAI CTGCAG 1 cut(s) 2548
BspOI GCTAGC 1 cut(s) 191
BspQI GCTCTTC 1 cut(s) 2079
BspT104I TTCGAA 2 cut(s) 283, 5344
BspT107I GGYRCC 3 cut(s) 484, 1609, 3587
BspTI CTTAAG 1 cut(s) 4199
BsrBI CCGCTC 1 cut(s) 5199
BsrDI GCAATG 5 cut(s) 467, 1046, 2091, 3971, 4243
BsrFI RCCGGY 4 cut(s) 534, 3727, 4381, 4781
BsrGI TGTACA 2 cut(s) 3968, 4986
BsrI ACTGG 5 cut(s) 977, 1799, 2316, 3442, 4593
BssAI RCCGGY 4 cut(s) 534, 3727, 4381, 4781
BssNI GRCGYC 3 cut(s) 346, 3388, 4353
BssSI CACGAG 2 cut(s) 611, 4752
BssT1I CCWWGG 8 cut(s) 178, 739, 1605, 3518, 3806, 4138, 4367, 4633
Bst2BI CACGAG 2 cut(s) 611, 4752
Bst6I CTCTTC 3 cut(s) 177, 2079, 4622
BstACI GRCGYC 3 cut(s) 346, 3388, 4353
BstAFI CTTAAG 1 cut(s) 4199
BstAPI GCANNNNNTGC 3 cut(s) 473, 1440, 5152
BstAUI TGTACA 2 cut(s) 3968, 4986
BstBAI YACGTR 2 cut(s) 86, 2167
BstBI TTCGAA 2 cut(s) 283, 5344
BstDSI CCRYGG 4 cut(s) 178, 1605, 3806, 4633
BstFNI CGCG 2 cut(s) 1671, 2151
BstH2I RGCGCY 2 cut(s) 167, 3802
BstHHI GCGC 7 cut(s) 166, 506, 1137, 3801, 4250, 4455, 5662
BstMAI GTCTC 8 cut(s) 277, 774, 1055, 1735, 3690, 3937, 4669, 4864
BstNSI RCATGY 5 cut(s) 2958, 4414, 4571, 4850, 4923
BstSFI CTRYAG 3 cut(s) 2176, 2544, 5376
BstSLI GKGCMC 2 cut(s) 489, 1205
BstUI CGCG 2 cut(s) 1671, 2151
BstV2I GAAGAC 2 cut(s) 1111, 4112
BstX2I RGATCY 9 cut(s) 321, 402, 598, 847, 1254, 1710, 3376, 4051, 4612
BstXI CCANNNNNNTGG 4 cut(s) 589, 1030, 4422, 4640
BstYI RGATCY 9 cut(s) 321, 402, 598, 847, 1254, 1710, 3376, 4051, 4612
BsuI GTATCC 2 cut(s) 445, 3181
BtgI CCRYGG 4 cut(s) 178, 1605, 3806, 4633
BtsI GCAGTG 1 cut(s) 4296
CaiI CAGNNNCTG 1 cut(s) 249
CciI TCATGA 5 cut(s) 307, 946, 1180, 2947, 5257
CfoI GCGC 7 cut(s) 166, 506, 1137, 3801, 4250, 4455, 5662
Cfr10I RCCGGY 4 cut(s) 534, 3727, 4381, 4781
Cfr13I GGNCC 9 cut(s) 579, 1019, 1421, 2993, 3175, 4757, 5263, 5297, 5760
CseI GACGC 4 cut(s) 75, 241, 335, 2157
CspAI ACCGGT 2 cut(s) 534, 4381
CspCI CAANNNNNGTGG 2 cut(s) 3805, 3840
DraI TTTAAA 2 cut(s) 4162, 4870
DrdI GACNNNNNNGTC 3 cut(s) 1017, 4352, 5482
DseDI GACNNNNNNGTC 3 cut(s) 1017, 4352, 5482
EaeI YGGCCR 5 cut(s) 264, 1471, 1749, 2938, 5314
Eam1104I CTCTTC 3 cut(s) 177, 2079, 4622
EarI CTCTTC 3 cut(s) 177, 2079, 4622
EciI GGCGGA 1 cut(s) 5153
Ecl136II GAGCTC 2 cut(s) 633, 2324
Eco130I CCWWGG 8 cut(s) 178, 739, 1605, 3518, 3806, 4138, 4367, 4633
Eco147I AGGCCT 2 cut(s) 3579, 4824
Eco24I GRGCYC 3 cut(s) 635, 2326, 4936
Eco32I GATATC 1 cut(s) 2233
Eco47I GGWCC 6 cut(s) 579, 1019, 1421, 2993, 3175, 4757
Eco47III AGCGCT 1 cut(s) 165
Eco53kI GAGCTC 2 cut(s) 633, 2324
Eco57I CTGAAG 6 cut(s) 765, 812, 1578, 2246, 2270, 2373
Eco72I CACGTG 1 cut(s) 2167
Eco88I CYCGRG 1 cut(s) 617
EcoICRI GAGCTC 2 cut(s) 633, 2324
EcoO109I RGGNCCY 2 cut(s) 1019, 4757
EcoRI GAATTC 1 cut(s) 3426
EcoRV GATATC 1 cut(s) 2233
EcoT14I CCWWGG 8 cut(s) 178, 739, 1605, 3518, 3806, 4138, 4367, 4633
EcoT22I ATGCAT 5 cut(s) 2710, 2786, 4925, 5212, 5725
EcoT38I GRGCYC 3 cut(s) 635, 2326, 4936
ErhI CCWWGG 8 cut(s) 178, 739, 1605, 3518, 3806, 4138, 4367, 4633
Esp3I CGTCTC 2 cut(s) 277, 3937
FalI AAGNNNNNCTT 6 cut(s) 1241, 1273, 2457, 2489, 3405, 3437
FaqI GGGAC 3 cut(s) 387, 1005, 4325
FauI CCCGC 2 cut(s) 611, 5192
FauNDI CATATG 2 cut(s) 2786, 5857
FbaI TGATCA 1 cut(s) 673
FblI GTMKAC 2 cut(s) 1674, 3332
FriOI GRGCYC 3 cut(s) 635, 2326, 4936
FspAI RTGCGCAY 1 cut(s) 1136
FspI TGCGCA 2 cut(s) 505, 1136
GlaI GCGC 7 cut(s) 165, 505, 1136, 3800, 4249, 4454, 5661
GsaI CCCAGC 3 cut(s) 116, 1891, 4695
GsuI CTGGAG 2 cut(s) 758, 1782
HaeII RGCGCY 2 cut(s) 167, 3802
HapII CCGG 7 cut(s) 276, 449, 535, 3728, 3744, 4382, 4782
HgaI GACGC 4 cut(s) 75, 241, 335, 2157
HhaI GCGC 7 cut(s) 166, 506, 1137, 3801, 4250, 4455, 5662
Hin1I GRCGYC 3 cut(s) 346, 3388, 4353
Hin6I GCGC 7 cut(s) 164, 504, 1135, 3799, 4248, 4453, 5660
HinP1I GCGC 7 cut(s) 164, 504, 1135, 3799, 4248, 4453, 5660
HincII GTYRAC 2 cut(s) 1854, 3363
HindII GTYRAC 2 cut(s) 1854, 3363
HindIII AAGCTT 1 cut(s) 3414
HpaI GTTAAC 1 cut(s) 1854
HpaII CCGG 7 cut(s) 276, 449, 535, 3728, 3744, 4382, 4782
Hpy99I CGWCG 5 cut(s) 23, 272, 351, 2889, 5311
HpyCH4IV ACGT 7 cut(s) 21, 85, 2166, 3388, 4061, 4353, 5309
HpySE526I ACGT 7 cut(s) 21, 85, 2166, 3388, 4061, 4353, 5309
Hsp92I GRCGYC 3 cut(s) 346, 3388, 4353
HspAI GCGC 7 cut(s) 164, 504, 1135, 3799, 4248, 4453, 5660
Ksp22I TGATCA 1 cut(s) 673
KspAI GTTAAC 1 cut(s) 1854
LguI GCTCTTC 1 cut(s) 2079
MaeII ACGT 7 cut(s) 21, 85, 2166, 3388, 4061, 4353, 5309
MbiI CCGCTC 1 cut(s) 5199
MfeI CAATTG 3 cut(s) 2064, 5736, 5755
MflI RGATCY 9 cut(s) 321, 402, 598, 847, 1254, 1710, 3376, 4051, 4612
MhlI GDGCHC 9 cut(s) 489, 618, 635, 1205, 2326, 3064, 4936, 5257, 5670
MlsI TGGCCA 3 cut(s) 1473, 1751, 2940
MluNI TGGCCA 3 cut(s) 1473, 1751, 2940
MlyI GAGTC 6 cut(s) 677, 1737, 4671, 4936, 5093, 5492
Mox20I TGGCCA 3 cut(s) 1473, 1751, 2940
Mph1103I ATGCAT 5 cut(s) 2710, 2786, 4925, 5212, 5725
MroXI GAANNNNTTC 2 cut(s) 3537, 3579
MscI TGGCCA 3 cut(s) 1473, 1751, 2940
MslI CAYNNNNRTG 5 cut(s) 1185, 2263, 2785, 4638, 5860
Msp20I TGGCCA 3 cut(s) 1473, 1751, 2940
MspA1I CMGCKG 3 cut(s) 258, 3067, 5225
MspCI CTTAAG 1 cut(s) 4199
MspI CCGG 7 cut(s) 276, 449, 535, 3728, 3744, 4382, 4782
MunI CAATTG 3 cut(s) 2064, 5736, 5755
Mva1269I GAATGC 7 cut(s) 1326, 1584, 2260, 2280, 3257, 3733, 3855
MvnI CGCG 2 cut(s) 1671, 2151
NciI CCSGG 2 cut(s) 277, 3744
NcoI CCATGG 4 cut(s) 178, 1605, 3806, 4633
NdeI CATATG 2 cut(s) 2786, 5857
NheI GCTAGC 1 cut(s) 187
NlaIV GGNNCC 7 cut(s) 486, 1021, 1603, 1611, 3279, 3589, 3814
NmuCI GTSAC 7 cut(s) 427, 640, 713, 1446, 2222, 2845, 2892
NsbI TGCGCA 2 cut(s) 505, 1136
NsiI ATGCAT 5 cut(s) 2710, 2786, 4925, 5212, 5725
NspI RCATGY 5 cut(s) 2958, 4414, 4571, 4850, 4923
NspV TTCGAA 2 cut(s) 283, 5344
OliI CACNNNNGTG 1 cut(s) 2263
PaeI GCATGC 1 cut(s) 4414
PaeR7I CTCGAG 1 cut(s) 617
PagI TCATGA 5 cut(s) 307, 946, 1180, 2947, 5257
PasI CCCWGGG 2 cut(s) 2730, 5763
PceI AGGCCT 2 cut(s) 3579, 4824
PciSI GCTCTTC 1 cut(s) 2079
PcsI WCGNNNNNNNCGW 2 cut(s) 346, 5154
PctI GAATGC 7 cut(s) 1326, 1584, 2260, 2280, 3257, 3733, 3855
PdmI GAANNNNTTC 2 cut(s) 3537, 3579
Pfl23II CGTACG 1 cut(s) 5310
PflMI CCANNNNNTGG 2 cut(s) 1758, 1894
PinAI ACCGGT 2 cut(s) 534, 4381
PleI GAGTC 6 cut(s) 677, 1736, 4671, 4935, 5092, 5491
PmaCI CACGTG 1 cut(s) 2167
PmlI CACGTG 1 cut(s) 2167
PpsI GAGTC 6 cut(s) 677, 1736, 4671, 4935, 5092, 5491
Ppu21I YACGTR 2 cut(s) 86, 2167
PpuMI RGGWCCY 2 cut(s) 1019, 4757
PshBI ATTAAT 1 cut(s) 5069
PsiI TTATAA 3 cut(s) 821, 965, 4322
Psp124BI GAGCTC 2 cut(s) 635, 2326
Psp5II RGGWCCY 2 cut(s) 1019, 4757
PspCI CACGTG 1 cut(s) 2167
PspFI CCCAGC 3 cut(s) 112, 1887, 4691
PspLI CGTACG 1 cut(s) 5310
PspN4I GGNNCC 7 cut(s) 486, 1021, 1603, 1611, 3279, 3589, 3814
PspPI GGNCC 9 cut(s) 579, 1019, 1421, 2993, 3175, 4757, 5263, 5297, 5760
PspPPI RGGWCCY 2 cut(s) 1019, 4757
PspXI VCTCGAGB 1 cut(s) 617
PstI CTGCAG 1 cut(s) 2548
PstNI CAGNNNCTG 1 cut(s) 249
PsuI RGATCY 9 cut(s) 321, 402, 598, 847, 1254, 1710, 3376, 4051, 4612
PvuII CAGCTG 2 cut(s) 258, 5225
RseI CAYNNNNRTG 5 cut(s) 1185, 2263, 2785, 4638, 5860
SacI GAGCTC 2 cut(s) 635, 2326
SapI GCTCTTC 1 cut(s) 2079
Sau96I GGNCC 9 cut(s) 579, 1019, 1421, 2993, 3175, 4757, 5263, 5297, 5760
ScaI AGTACT 2 cut(s) 907, 4009
SchI GAGTC 6 cut(s) 677, 1737, 4671, 4936, 5093, 5492
SduI GDGCHC 9 cut(s) 489, 618, 635, 1205, 2326, 3064, 4936, 5257, 5670
SfcI CTRYAG 3 cut(s) 2176, 2544, 5376
Sfr274I CTCGAG 1 cut(s) 617
SfuI TTCGAA 2 cut(s) 283, 5344
SinI GGWCC 6 cut(s) 579, 1019, 1421, 2993, 3175, 4757
SlaI CTCGAG 1 cut(s) 617
SmiMI CAYNNNNRTG 5 cut(s) 1185, 2263, 2785, 4638, 5860
SmlI CTYRAG 6 cut(s) 617, 1661, 2273, 2897, 3337, 4199
SmoI CTYRAG 6 cut(s) 617, 1661, 2273, 2897, 3337, 4199
SpeI ACTAGT 3 cut(s) 2875, 3235, 3629
SphI GCATGC 1 cut(s) 4414
SseBI AGGCCT 2 cut(s) 3579, 4824
SsiI CCGC 8 cut(s) 222, 544, 604, 2098, 2151, 3067, 5138, 5199
SspI AATATT 2 cut(s) 2336, 5817
SstI GAGCTC 2 cut(s) 635, 2326
StuI AGGCCT 2 cut(s) 3579, 4824
StyI CCWWGG 8 cut(s) 178, 739, 1605, 3518, 3806, 4138, 4367, 4633
TaiI ACGT 7 cut(s) 24, 88, 2169, 3391, 4064, 4356, 5312
TatI WGTACW 7 cut(s) 905, 2022, 2537, 2878, 3968, 4007, 4986
TauI GCSGC 1 cut(s) 224
TseFI GTSAC 7 cut(s) 427, 640, 713, 1446, 2222, 2845, 2892
Tsp45I GTSAC 7 cut(s) 427, 640, 713, 1446, 2222, 2845, 2892
TspGWI ACGGA 4 cut(s) 2067, 3961, 4118, 5306
Van91I CCANNNNNTGG 2 cut(s) 1758, 1894
Vha464I CTTAAG 1 cut(s) 4199
VpaK11BI GGWCC 6 cut(s) 579, 1019, 1421, 2993, 3175, 4757
VspI ATTAAT 1 cut(s) 5069
XceI RCATGY 5 cut(s) 2958, 4414, 4571, 4850, 4923
XcmI CCANNNNNNNNNTGG 2 cut(s) 1030, 5754
XhoI CTCGAG 1 cut(s) 617
XmaJI CCTAGG 1 cut(s) 3518
XmiI GTMKAC 2 cut(s) 1674, 3332
XmnI GAANNNNTTC 2 cut(s) 3537, 3579
ZraI GACGTC 2 cut(s) 3389, 4354
ZrmI AGTACT 2 cut(s) 907, 4009
Zsp2I ATGCAT 5 cut(s) 2710, 2786, 4925, 5212, 5725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.