MD00G1111100.v1.1

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000800
Physical Location & Seq
Forward (+)
23237992 .. 23239176
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1111100.v1.1.491

Sequence Viewer

Length: 273 bp
ATGACTGGATGGCATATGATGCAGGTGTTTAGTTCTTTGGGCGTAGCATCAGAGGCATTGAACTTATGTTGTAAGGCATCATGGACTCGTGTTATACGTCTTTGTGAGATGTTTGCACACAAACAGAAGGTGTCCCAGGTTGATCTGTCAGAAGATGCCATTGTAGATTTCTTTAATGAGTGCTGTACGAGAAGTGCTTTTCTTTTGGACGTTCTTAATGAATTGCACAGTTATGATGTGGAAAGAACTTTATTAGAGAGCTTTGAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

91

Amino Acids

10.34

Weight (kDa)

4.81

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 13
AfaI GTAC 1 cut(s) 187
AgsI TTSAA 2 cut(s) 61, 266
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 1 cut(s) 261
AluI AGCT 1 cut(s) 261
BauI CACGAG 1 cut(s) 87
BccI CCATC 1 cut(s) 3
BciT130I CCWGG 1 cut(s) 137
BfuAI ACCTGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 137
BmrFI CCNGG 1 cut(s) 137
BmsI GCATC 4 cut(s) 9, 56, 86, 145
BsaJI CCNNGG 1 cut(s) 135
Bse1I ACTGG 1 cut(s) 10
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 1 cut(s) 14
BseNI ACTGG 1 cut(s) 10
BslFI GGGAC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 118
Bsp143I GATC 1 cut(s) 142
BspMI ACCTGC 1 cut(s) 13
BsrI ACTGG 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 1 cut(s) 142
BssSI CACGAG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 1 cut(s) 230
BstAPI GCANNNNNTGC 1 cut(s) 19
BstF5I GGATG 1 cut(s) 14
BstKTI GATC 1 cut(s) 145
BstMBI GATC 1 cut(s) 142
BstMWI GCNNNNNNNGC 2 cut(s) 19, 53
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 135
BtsCI GGATG 1 cut(s) 14
BveI ACCTGC 1 cut(s) 13
Csp6I GTAC 1 cut(s) 186
CviAII CATG 1 cut(s) 81
CviJI RGCY 1 cut(s) 261
CviKI_1 RGCY 1 cut(s) 261
CviQI GTAC 1 cut(s) 186
DpnI GATC 1 cut(s) 144
DpnII GATC 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 135
FaeI CATG 1 cut(s) 84
FaiI YATR 6 cut(s) 15, 17, 67, 82, 95, 234
FaqI GGGAC 1 cut(s) 118
FatI CATG 1 cut(s) 80
FauNDI CATATG 1 cut(s) 15
FokI GGATG 1 cut(s) 21
Hin1II CATG 1 cut(s) 84
HinfI GANTC 1 cut(s) 85
Hpy188I TCNGA 2 cut(s) 52, 151
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4IV ACGT 2 cut(s) 97, 210
HpyCH4V TGCA 3 cut(s) 22, 116, 226
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 53
HpySE526I ACGT 2 cut(s) 97, 210
Hsp92II CATG 1 cut(s) 84
Kzo9I GATC 1 cut(s) 142
LpnPI CCDG 3 cut(s) 8, 122, 149
LweI GCATC 4 cut(s) 9, 56, 86, 145
MaeII ACGT 2 cut(s) 97, 210
MalI GATC 1 cut(s) 144
MboI GATC 1 cut(s) 142
MboII GAAGA 1 cut(s) 164
MluCI AATT 2 cut(s) 221, 268
MlyI GAGTC 1 cut(s) 79
MnlI CCTC 1 cut(s) 46
MseI TTAA 2 cut(s) 174, 216
MslI CAYNNNNRTG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 137
MvaI CCWGG 1 cut(s) 137
MwoI GCNNNNNNNGC 2 cut(s) 19, 53
NdeI CATATG 1 cut(s) 15
NdeII GATC 1 cut(s) 142
NlaIII CATG 1 cut(s) 84
PaqCI CACCTGC 1 cut(s) 13
PcsI WCGNNNNNNNCGW 1 cut(s) 94
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
RseI CAYNNNNRTG 1 cut(s) 231
SaqAI TTAA 2 cut(s) 174, 216
Sau3AI GATC 1 cut(s) 142
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 1 cut(s) 137
SetI ASST 6 cut(s) 27, 100, 132, 141, 213, 263
SfaNI GCATC 4 cut(s) 9, 56, 86, 145
SgeI CNNG 8 cut(s) 18, 35, 93, 99, 101, 148, 149, 201
SmiMI CAYNNNNRTG 1 cut(s) 231
Sse9I AATT 2 cut(s) 221, 268
StyD4I CCNGG 1 cut(s) 135
TaaI ACNGT 1 cut(s) 230
TaiI ACGT 2 cut(s) 100, 213
TasI AATT 2 cut(s) 221, 268
Tru1I TTAA 2 cut(s) 174, 216
Tru9I TTAA 2 cut(s) 174, 216
TspDTI ATGAA 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.