FvH4_5g29930

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000800
Physical Location & Seq
Reverse (-)
20800676 .. 20808223
7548 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29930.t1

Sequence Viewer

Length: 888 bp
ATGGCATCATGTAATGATTCTAATGGTGGTGATAACTTGTCTGAGTGGGCCTACAAACTAGTATCAGAAGCAGTGAATGAGCTTAGAGGGTTACATTCATCTGTTAATATAAAGCCAACAATATTAGTTCTGGACTACGAGGTGCAGATGCTTCCTTGGGAGAAGTTACCAATCCTTAGAAACCAGGAGGTGTATCGCATGCCCTCTGTTGCGAGCATTTTGGCAACAGCCAATAGAAACTACCAGAATCAAGATCAACTTCAGAGTATACCGACCCCCTTATTTCCTTGGATTGATCCCTTGGATGCCTTTTATCTTCTGAATCCCAGTGGTGATCTAAATTATACGCAAAATGAATTTGAGACATGGTTCAGAGATCAAAACTTGGAGGGGAAGGCTGGATGTGCACCTCCTGCTGAAGAATTGGCAGTAGCGTTGAAAAGCCATGATCTATTTATGTATTTTGGCCATGGATGTGGGACACAATATATTCCTAGACGTGAGATTCAGAAACTGGAACGCTGTGCCGCAACTCTCCTGATGGGATGTAGTAGTGGTTCTCTGAAACTGAATGGATGTTATGTTCCACAAGGTACTCCATTATCCTATTTGCTGGCCGGTTCTCCTGTCAATGTCGCCAATTTGTGGGATGTGACGGACAGGGATATTGACCGATTTGCAAAGTCCATGCTTGATTCTTGGTTGAAAGCAAGGTCTAGTCCCTGTGTTGGTTGTGCCCAATGCAACAAAAACCTGTCTTGTGAGCATAGGCCGACGGTTGGATCATTCATGAGTGAAGCACGTAAAACTTGCCAGTTACCTTTCCTGATTGGAGCAGCCCCTGTATGCTATGGTGTCCCAACTGGCATATGGAAAAAAGAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

296

Amino Acids

32.84

Weight (kDa)

5.65

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Separin_C PF03568 33 - 223 3.9e-73 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 645
AccI GTMKAC 1 cut(s) 268
AciI CCGC 1 cut(s) 528
AclWI GGATC 2 cut(s) 290, 790
AcoI YGGCCR 2 cut(s) 466, 615
AcsI RAATTY 1 cut(s) 356
AcuI CTGAAG 2 cut(s) 245, 438
AfaI GTAC 1 cut(s) 595
AfiI CCNNNNNNNGG 3 cut(s) 645, 728, 779
AgsI TTSAA 2 cut(s) 439, 706
AhlI ACTAGT 1 cut(s) 58
AjiI CACGTC 1 cut(s) 500
AjnI CCWGG 1 cut(s) 183
AloI GAACNNNNNNTCC 2 cut(s) 567, 599
AluBI AGCT 1 cut(s) 82
AluI AGCT 1 cut(s) 82
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 1 cut(s) 356
Alw44I GTGCAC 1 cut(s) 405
AlwI GGATC 2 cut(s) 290, 790
AlwNI CAGNNNCTG 2 cut(s) 514, 842
AoxI GGCC 4 cut(s) 48, 466, 615, 770
ApaLI GTGCAC 1 cut(s) 405
ApeKI GCWGC 1 cut(s) 836
ApoI RAATTY 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 48
AsuHPI GGTGA 2 cut(s) 41, 344
BaeGI GKGCMC 2 cut(s) 409, 739
BalI TGGCCA 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 1 cut(s) 848
BccI CCATC 1 cut(s) 535
BciT130I CCWGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 356
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 3 cut(s) 59, 495, 717
BisI GCNGC 2 cut(s) 528, 837
BlsI GCNGC 2 cut(s) 529, 838
Bme1390I CCNGG 1 cut(s) 185
BmgBI CACGTC 1 cut(s) 500
BmgT120I GGNCC 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 185
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 3 cut(s) 14, 138, 295
BmuI ACTGGG 1 cut(s) 321
BsaAI YACGTR 1 cut(s) 803
BsaJI CCNNGG 4 cut(s) 155, 287, 300, 469
Bsc4I CCNNNNNNNGG 3 cut(s) 645, 728, 779
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 4 cut(s) 327, 519, 814, 868
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 4 cut(s) 155, 287, 300, 469
BseGI GGATG 6 cut(s) 310, 407, 479, 551, 581, 655
BseLI CCNNNNNNNGG 3 cut(s) 645, 728, 779
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 4 cut(s) 327, 519, 814, 868
BseSI GKGCMC 2 cut(s) 409, 739
BseXI GCAGC 1 cut(s) 848
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 4 cut(s) 50, 468, 617, 772
BsiHKAI GWGCWC 1 cut(s) 409
BsiSI CCGG 1 cut(s) 618
BslFI GGGAC 3 cut(s) 493, 705, 842
BslI CCNNNNNNNGG 3 cut(s) 645, 728, 779
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 3 cut(s) 493, 705, 842
BsnI GGCC 4 cut(s) 50, 468, 617, 772
Bsp1286I GDGCHC 2 cut(s) 409, 739
Bsp143I GATC 6 cut(s) 253, 295, 334, 376, 448, 782
Bsp19I CCATGG 1 cut(s) 469
BspACI CCGC 1 cut(s) 528
BspANI GGCC 4 cut(s) 50, 468, 617, 772
BspCNI CTCAG 1 cut(s) 34
BspHI TCATGA 1 cut(s) 789
BspPI GGATC 2 cut(s) 290, 790
BsrFI RCCGGY 1 cut(s) 617
BsrI ACTGG 4 cut(s) 327, 519, 814, 868
BssAI RCCGGY 1 cut(s) 617
BssECI CCNNGG 4 cut(s) 155, 287, 300, 469
BssMI GATC 6 cut(s) 253, 295, 334, 376, 448, 782
BssNAI GTATAC 1 cut(s) 269
BssT1I CCWWGG 4 cut(s) 155, 287, 300, 469
Bst1107I GTATAC 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 1 cut(s) 778
BstAPI GCANNNNNTGC 1 cut(s) 413
BstBAI YACGTR 1 cut(s) 803
BstC8I GCNNGC 3 cut(s) 200, 214, 615
BstDEI CTNAG 3 cut(s) 42, 83, 176
BstDSI CCRYGG 1 cut(s) 469
BstF5I GGATG 6 cut(s) 310, 407, 479, 551, 581, 655
BstKTI GATC 6 cut(s) 256, 298, 337, 379, 451, 785
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 6 cut(s) 253, 295, 334, 376, 448, 782
BstMWI GCNNNNNNNGC 2 cut(s) 404, 413
BstNI CCWGG 1 cut(s) 185
BstNSI RCATGY 1 cut(s) 202
BstSCI CCNGG 1 cut(s) 183
BstSLI GKGCMC 2 cut(s) 409, 739
BstV1I GCAGC 1 cut(s) 848
BstXI CCANNNNNNTGG 1 cut(s) 476
BstZ17I GTATAC 1 cut(s) 269
BsuRI GGCC 4 cut(s) 50, 468, 617, 772
BtgI CCRYGG 1 cut(s) 469
BtrI CACGTC 1 cut(s) 500
BtsCI GGATG 6 cut(s) 310, 407, 479, 551, 581, 655
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 2 cut(s) 78, 334
Cac8I GCNNGC 3 cut(s) 200, 214, 615
CaiI CAGNNNCTG 2 cut(s) 514, 842
CciI TCATGA 1 cut(s) 789
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 594
CviAII CATG 7 cut(s) 9, 199, 366, 446, 470, 688, 790
CviQI GTAC 1 cut(s) 594
DdeI CTNAG 3 cut(s) 42, 83, 176
DpnI GATC 6 cut(s) 255, 297, 336, 378, 450, 784
DpnII GATC 6 cut(s) 253, 295, 334, 376, 448, 782
EaeI YGGCCR 2 cut(s) 466, 615
Eco130I CCWWGG 4 cut(s) 155, 287, 300, 469
Eco57I CTGAAG 2 cut(s) 245, 438
EcoRII CCWGG 1 cut(s) 183
EcoT14I CCWWGG 4 cut(s) 155, 287, 300, 469
ErhI CCWWGG 4 cut(s) 155, 287, 300, 469
FaeI CATG 7 cut(s) 12, 202, 369, 449, 473, 691, 793
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FaqI GGGAC 3 cut(s) 493, 705, 842
FatI CATG 7 cut(s) 8, 198, 365, 445, 469, 687, 789
FauNDI CATATG 1 cut(s) 869
FblI GTMKAC 1 cut(s) 268
Fnu4HI GCNGC 2 cut(s) 528, 837
FokI GGATG 6 cut(s) 317, 414, 486, 558, 588, 662
Fsp4HI GCNGC 2 cut(s) 528, 837
FspBI CTAG 3 cut(s) 59, 495, 717
GluI GCNGC 2 cut(s) 528, 837
HaeIII GGCC 4 cut(s) 50, 468, 617, 772
HapII CCGG 1 cut(s) 618
Hin1II CATG 7 cut(s) 12, 202, 369, 449, 473, 691, 793
HinfI GANTC 5 cut(s) 17, 247, 322, 505, 695
HpaII CCGG 1 cut(s) 618
HphI GGTGA 2 cut(s) 41, 344
Hpy166II GTNNAC 2 cut(s) 269, 407
Hpy188I TCNGA 7 cut(s) 43, 67, 264, 321, 374, 510, 564
Hpy188III TCNNGA 5 cut(s) 131, 251, 538, 790, 826
Hpy8I GTNNAC 2 cut(s) 269, 407
Hpy99I CGWCG 1 cut(s) 778
HpyAV CCTTC 1 cut(s) 388
HpyCH4III ACNGT 1 cut(s) 778
HpyCH4IV ACGT 2 cut(s) 499, 802
HpyCH4V TGCA 4 cut(s) 145, 407, 680, 744
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 413
HpyF3I CTNAG 3 cut(s) 42, 83, 176
HpySE526I ACGT 2 cut(s) 499, 802
Hsp92II CATG 7 cut(s) 12, 202, 369, 449, 473, 691, 793
Kzo9I GATC 6 cut(s) 253, 295, 334, 376, 448, 782
LmnI GCTCC 1 cut(s) 833
Lsp1109I GCAGC 1 cut(s) 848
LweI GCATC 3 cut(s) 14, 138, 295
MaeI CTAG 3 cut(s) 59, 495, 717
MaeII ACGT 2 cut(s) 499, 802
MaeIII GTNAC 4 cut(s) 90, 165, 652, 816
MalI GATC 6 cut(s) 255, 297, 336, 378, 450, 784
MboI GATC 6 cut(s) 253, 295, 334, 376, 448, 782
MboII GAAGA 2 cut(s) 308, 431
MhlI GDGCHC 2 cut(s) 409, 739
MlsI TGGCCA 1 cut(s) 468
MluCI AATT 4 cut(s) 340, 356, 422, 640
MluNI TGGCCA 1 cut(s) 468
MmeI TCCRAC 1 cut(s) 760
MnlI CCTC 6 cut(s) 80, 133, 181, 214, 382, 420
Mox20I TGGCCA 1 cut(s) 468
MscI TGGCCA 1 cut(s) 468
MseI TTAA 1 cut(s) 105
MslI CAYNNNNRTG 1 cut(s) 474
Msp20I TGGCCA 1 cut(s) 468
MspI CCGG 1 cut(s) 618
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MwoI GCNNNNNNNGC 2 cut(s) 404, 413
NcoI CCATGG 1 cut(s) 469
NdeI CATATG 1 cut(s) 869
NdeII GATC 6 cut(s) 253, 295, 334, 376, 448, 782
NlaIII CATG 7 cut(s) 12, 202, 369, 449, 473, 691, 793
NmuCI GTSAC 1 cut(s) 652
NspI RCATGY 1 cut(s) 202
PaeI GCATGC 1 cut(s) 202
PagI TCATGA 1 cut(s) 789
PfeI GAWTC 5 cut(s) 17, 247, 322, 505, 695
PflMI CCANNNNNTGG 1 cut(s) 645
PkrI GCNGC 2 cut(s) 529, 838
Ppu21I YACGTR 1 cut(s) 803
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspPI GGNCC 1 cut(s) 48
PsrI GAACNNNNNNTAC 2 cut(s) 541, 573
PstNI CAGNNNCTG 2 cut(s) 514, 842
RsaI GTAC 1 cut(s) 595
RsaNI GTAC 1 cut(s) 594
RseI CAYNNNNRTG 1 cut(s) 474
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 2 cut(s) 528, 837
Sau3AI GATC 6 cut(s) 253, 295, 334, 376, 448, 782
Sau96I GGNCC 1 cut(s) 48
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 2 cut(s) 409, 739
SfaNI GCATC 3 cut(s) 14, 138, 295
SmiMI CAYNNNNRTG 1 cut(s) 474
SpeI ACTAGT 1 cut(s) 58
SphI GCATGC 1 cut(s) 202
Sse9I AATT 4 cut(s) 340, 356, 422, 640
SsiI CCGC 1 cut(s) 528
SspI AATATT 1 cut(s) 123
SspMI CTAG 3 cut(s) 59, 495, 717
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 4 cut(s) 155, 287, 300, 469
TaaI ACNGT 1 cut(s) 778
TaiI ACGT 2 cut(s) 502, 805
TaqII GACCGA 1 cut(s) 687
TasI AATT 4 cut(s) 340, 356, 422, 640
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 5 cut(s) 17, 247, 322, 505, 695
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 2 cut(s) 78, 334
TseFI GTSAC 1 cut(s) 652
TseI GCWGC 1 cut(s) 836
Tsp45I GTSAC 1 cut(s) 652
TspDTI ATGAA 3 cut(s) 87, 369, 778
TspGWI ACGGA 1 cut(s) 671
TspRI CASTG 2 cut(s) 78, 334
Van91I CCANNNNNTGG 1 cut(s) 645
VneI GTGCAC 1 cut(s) 405
XapI RAATTY 1 cut(s) 356
XceI RCATGY 1 cut(s) 202
XcmI CCANNNNNNNNNTGG 1 cut(s) 867
XmiI GTMKAC 1 cut(s) 268
XspI CTAG 3 cut(s) 59, 495, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.