Rroxscaffold_3G00237070

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000800
Physical Location & Seq
Reverse (-)
24577420 .. 24582340
4921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00237070.1

Sequence Viewer

Length: 1743 bp
ATGGCCTCCGCCACCGAAACCTCCGAAACTCTCCTCTCCAAGCTCGAATCCGCCGATTCCGCCGGGATTCATCGTCTCATCTCCGACTACCTAAACCCATTCGCCGACCTTCACAAAAAGAAGACCTCAACCGACCACCAAACCCTACAACGCTCTCTCGCCAAAAAATTCACCCCCTTCCTCAACCGGGCCTTAGCCATCCTCCCCAAACGGCTCGCCGATCCTTCAAAGCTAGATGCCAAATTTGCCCCCGAATTGTTCGATATCTACCGCCTCTGCCTCGACTCCATGGACGCCGTCTCCTCCCAATTTTCCGGCAAGCCCTACAAGTTTCACATCCAGAGGATCCGGTTGGTTTATGCATTCGAGGCCTGGGGCCGCCACAAGGACGCCGAGTCCGAGGCGCTTAGGGTTTTGAAGAGCTTCAAGGCGATTGAATTAGGAACGAAACCGGTGAATTTGGACCGTAGATTTGTGCCAGGTGTCGAGCAGGGAGGCTGCGATAAGGAGTTTGGGGCTTTGCTTGGAGAAGTGGTGTGGATGCTAGCCAAGTGTGCTTCGTTAATTCAAAGCAAGGAAGCCGAGGTGTATGAGCGGCTGCTTTGTATGGTTGAGGAGTTGGGACCTTGGTTTAGGGTGTTAGATGAAACTGTACATGACAAGGTGCACAGACAGCTTGCCATTTATCTCGAGAGGTGTACTCTTTTTTTAGTCCGGGAGCTATCATCCTTGGGAGCAAATCTCGTATGCAAGTTCTGCCGCATAACAGTGAGCGAGTATGCAAAGTCATCCATCAAAGATCATATGTTCAAGTGCGCCCGTCGAATATGTTCAGCTGTATTTTCATTACAAGAGACTAGTTCCTCATTGATCAGTAACACATTGTTATGCTTGTTTGACTCCCTTGTTGATGAATGCAAGGCTGAGGTCGAATATTCAGGAAAAGATTTTATTGAACTTATTGCTTATTGTGCCAATAAGTGTCGAACTGCAAGTACAAATCTTTCTGGTACTATGAGATCTCATTCAAAAAAATTAATAACTCACTTTGATCAGGATCCACCTTTAAATTTGATTCTAAGACTTTATGCTACTGGTTTGCACTACGTTGATTGTAGTATAAAGTCAAAGGACGAGGATCTTACATCTTCTGGAGGTGCAATTAGAATGTTGCTTGATGATGAGGATACAATGACTGATTTGTCAGATTGTCTTGATTCATTAGAAAGTTATTTCCAAATTGGTACAAATGATATTTCCAAAAGTGGGTTTTCCACGAGTCACGTGCAGAAGGATTCAAAGGATTGTCTTCTGCCTTATTTTAATGCATTGGAGTTCTTATGCCATCCACTTGCTGACTTAGTATATTCGGGAAAGAAACAGATTTTTGCTGACAATGGAGCTGCCTCTGTTTCTAGTGAACTGTGTGTTATCCAGGGTGCATTTCATCAGTTATATGATTTGTTCCTTTCTTTACAAACGTACAAAATTACTTGTGAGATAGATATAGATGGATTTAAGCACAACAGCATCCTTCGTGCTTCTGTGGCTGCTTTTATACTTTCCACCAGAACGAAGCTTAACATGCAGAAGAGTGCACAAATACTGAACCATGTCATTACCAGCGAATGGATTCAACTTGATGGTCTAAAGCATATATATGTCTCTCTCTACAATACTGGTACACGTTTGTACAAAAACAAGGAGCTGAATGAGGTATTGTTTTACAGAATGATATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

580

Amino Acids

65.49

Weight (kDa)

6.63

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 9 - 279 1.2e-106 Separase-like, TPR repeats
TPR_ESP1_2nd PF25113 289 - 462 2.9e-41 Separase-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1201
AccB7I CCANNNNNTGG 1 cut(s) 1629
AccBSI CCGCTC 1 cut(s) 595
AciI CCGC 7 cut(s) 9, 51, 60, 271, 379, 595, 760
AclWI GGATC 6 cut(s) 215, 340, 353, 1052, 1065, 1146
AcsI RAATTY 4 cut(s) 167, 242, 457, 1069
AcvI CACGTG 1 cut(s) 1285
AcyI GRCGYC 2 cut(s) 294, 390
AfaI GTAC 8 cut(s) 654, 700, 997, 1012, 1246, 1484, 1684, 1694
AfiI CCNNNNNNNGG 4 cut(s) 187, 385, 1266, 1629
AflIII ACRYGT 1 cut(s) 1685
AgeI ACCGGT 1 cut(s) 451
AhdI GACNNNNNGTC 1 cut(s) 394
AhlI ACTAGT 1 cut(s) 857
AjnI CCWGG 3 cut(s) 371, 478, 1434
AluBI AGCT 9 cut(s) 43, 232, 423, 676, 721, 836, 1403, 1579, 1708
AluI AGCT 9 cut(s) 43, 232, 423, 676, 721, 836, 1403, 1579, 1708
Alw21I GWGCWC 2 cut(s) 669, 1600
Alw26I GTCTC 4 cut(s) 80, 304, 848, 1669
Alw44I GTGCAC 2 cut(s) 665, 1596
AlwI GGATC 6 cut(s) 215, 340, 353, 1052, 1065, 1146
Ama87I CYCGRG 1 cut(s) 689
AoxI GGCC 4 cut(s) 3, 189, 369, 376
ApaLI GTGCAC 2 cut(s) 665, 1596
ApeKI GCWGC 4 cut(s) 498, 598, 1403, 1550
ApoI RAATTY 4 cut(s) 167, 242, 457, 1069
ArsI GACNNNNNNTTYG 2 cut(s) 455, 487
AseI ATTAAT 1 cut(s) 1037
AsiGI ACCGGT 1 cut(s) 451
Asp700I GAANNNNTTC 3 cut(s) 422, 829, 1632
AspLEI GCGC 2 cut(s) 406, 818
AspS9I GGNCC 4 cut(s) 189, 376, 463, 623
AsuC2I CCSGG 3 cut(s) 64, 188, 716
AsuHPI GGTGA 2 cut(s) 163, 466
AsuNHI GCTAGC 1 cut(s) 544
AvaI CYCGRG 1 cut(s) 689
AvaII GGWCC 2 cut(s) 463, 623
BaeGI GKGCMC 2 cut(s) 669, 1600
BamHI GGATCC 2 cut(s) 345, 1057
BauI CACGAG 1 cut(s) 1276
BbrPI CACGTG 1 cut(s) 1285
BbsI GAAGAC 2 cut(s) 128, 1301
Bbv12I GWGCWC 2 cut(s) 669, 1600
BbvCI CCTCAGC 1 cut(s) 924
BbvI GCAGC 4 cut(s) 485, 585, 1390, 1537
BccI CCATC 5 cut(s) 206, 800, 1353, 1505, 1637
BceAI ACGGC 2 cut(s) 227, 281
BcgI CGANNNNNNTGC 2 cut(s) 1267, 1301
BciT130I CCWGG 3 cut(s) 373, 480, 1436
BciVI GTATCC 1 cut(s) 1180
BclI TGATCA 2 cut(s) 870, 1051
BcnI CCSGG 3 cut(s) 64, 188, 716
BcoDI GTCTC 4 cut(s) 80, 304, 848, 1669
BcuI ACTAGT 1 cut(s) 857
BfaI CTAG 4 cut(s) 233, 545, 858, 1416
BfoI RGCGCY 1 cut(s) 407
BfuI GTATCC 1 cut(s) 1180
BglII AGATCT 1 cut(s) 1019
BisI GCNGC 7 cut(s) 379, 499, 596, 599, 760, 1404, 1551
BlsI GCNGC 7 cut(s) 380, 500, 597, 600, 761, 1405, 1552
Bme1390I CCNGG 6 cut(s) 64, 188, 373, 480, 716, 1436
Bme18I GGWCC 2 cut(s) 463, 623
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 689
BmgT120I GGNCC 4 cut(s) 189, 376, 463, 623
BmiI GGNNCC 4 cut(s) 347, 377, 624, 1059
BmrFI CCNGG 6 cut(s) 64, 188, 373, 480, 716, 1436
BmsI GCATC 3 cut(s) 226, 531, 1539
BmtI GCTAGC 1 cut(s) 548
BpiI GAAGAC 2 cut(s) 128, 1301
BplI GAGNNNNNCTC 4 cut(s) 685, 717, 726, 758
BpmI CTGGAG 1 cut(s) 1173
Bpu10I CCTNAGC 3 cut(s) 193, 407, 924
BpuMI CCSGG 3 cut(s) 64, 188, 716
BsaAI YACGTR 1 cut(s) 1285
BsaBI GATNNNNATC 1 cut(s) 1056
BsaHI GRCGYC 2 cut(s) 294, 390
BsaJI CCNNGG 7 cut(s) 288, 372, 399, 582, 626, 729, 1435
BsaWI WCCGGW 2 cut(s) 348, 451
BsaXI ACNNNNNCTCC 4 cut(s) 284, 314, 519, 549
Bsc4I CCNNNNNNNGG 4 cut(s) 187, 385, 1266, 1629
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 2 cut(s) 1099, 1684
Bse8I GATNNNNATC 1 cut(s) 1056
BseBI CCWGG 3 cut(s) 373, 480, 1436
BseDI CCNNGG 7 cut(s) 288, 372, 399, 582, 626, 729, 1435
BseGI GGATG 7 cut(s) 198, 336, 546, 725, 788, 1345, 1530
BseJI GATNNNNATC 1 cut(s) 1056
BseLI CCNNNNNNNGG 4 cut(s) 187, 385, 1266, 1629
BseMII CTCAG 1 cut(s) 915
BseNI ACTGG 2 cut(s) 1099, 1684
BseRI GAGGAG 3 cut(s) 23, 292, 629
BseSI GKGCMC 2 cut(s) 669, 1600
BseXI GCAGC 4 cut(s) 485, 585, 1390, 1537
BsgI GTGCAG 1 cut(s) 1307
BshFI GGCC 4 cut(s) 5, 191, 371, 378
BshTI ACCGGT 1 cut(s) 451
BsiHKAI GWGCWC 2 cut(s) 669, 1600
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 6 cut(s) 63, 187, 315, 349, 452, 715
BslFI GGGAC 1 cut(s) 636
BslI CCNNNNNNNGG 4 cut(s) 187, 385, 1266, 1629
BsmAI GTCTC 4 cut(s) 80, 304, 848, 1669
BsmBI CGTCTC 2 cut(s) 80, 304
BsmFI GGGAC 1 cut(s) 636
BsmI GAATGC 2 cut(s) 362, 920
BsnI GGCC 4 cut(s) 5, 191, 371, 378
BsoBI CYCGRG 1 cut(s) 689
Bsp1286I GDGCHC 2 cut(s) 669, 1600
Bsp1407I TGTACA 2 cut(s) 652, 1692
Bsp143I GATC 8 cut(s) 220, 345, 799, 870, 1019, 1051, 1057, 1138
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 7 cut(s) 9, 51, 60, 271, 379, 595, 760
BspANI GGCC 4 cut(s) 5, 191, 371, 378
BspCNI CTCAG 1 cut(s) 916
BspLI GGNNCC 4 cut(s) 347, 377, 624, 1059
BspOI GCTAGC 1 cut(s) 548
BspPI GGATC 6 cut(s) 215, 340, 353, 1052, 1065, 1146
BspQI GCTCTTC 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 595
BsrFI RCCGGY 1 cut(s) 451
BsrGI TGTACA 2 cut(s) 652, 1692
BsrI ACTGG 2 cut(s) 1099, 1684
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 7 cut(s) 288, 372, 399, 582, 626, 729, 1435
BssMI GATC 8 cut(s) 220, 345, 799, 870, 1019, 1051, 1057, 1138
BssNI GRCGYC 2 cut(s) 294, 390
BssSI CACGAG 1 cut(s) 1276
BssT1I CCWWGG 3 cut(s) 288, 626, 729
Bst2BI CACGAG 1 cut(s) 1276
Bst2UI CCWGG 3 cut(s) 373, 480, 1436
Bst4CI ACNGT 4 cut(s) 467, 652, 769, 1425
Bst6I CTCTTC 2 cut(s) 413, 1586
BstACI GRCGYC 2 cut(s) 294, 390
BstAPI GCANNNNNTGC 1 cut(s) 756
BstAUI TGTACA 2 cut(s) 652, 1692
BstBAI YACGTR 1 cut(s) 1285
BstC8I GCNNGC 4 cut(s) 216, 320, 546, 678
BstDEI CTNAG 5 cut(s) 193, 407, 924, 1079, 1360
BstDSI CCRYGG 1 cut(s) 288
BstF5I GGATG 7 cut(s) 198, 336, 546, 725, 788, 1345, 1530
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 2 cut(s) 406, 818
BstKTI GATC 8 cut(s) 223, 348, 802, 873, 1022, 1054, 1060, 1141
BstMAI GTCTC 4 cut(s) 80, 304, 848, 1669
BstMBI GATC 8 cut(s) 220, 345, 799, 870, 1019, 1051, 1057, 1138
BstMWI GCNNNNNNNGC 9 cut(s) 59, 245, 368, 554, 673, 756, 971, 1547, 1585
BstNI CCWGG 3 cut(s) 373, 480, 1436
BstNSI RCATGY 1 cut(s) 1588
BstSCI CCNGG 6 cut(s) 62, 186, 371, 478, 714, 1434
BstSLI GKGCMC 2 cut(s) 669, 1600
BstV1I GCAGC 4 cut(s) 485, 585, 1390, 1537
BstV2I GAAGAC 2 cut(s) 128, 1301
BstX2I RGATCY 4 cut(s) 345, 1019, 1057, 1138
BstYI RGATCY 4 cut(s) 345, 1019, 1057, 1138
BsuI GTATCC 1 cut(s) 1180
BsuRI GGCC 4 cut(s) 5, 191, 371, 378
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 7 cut(s) 198, 336, 546, 725, 788, 1345, 1530
BtsIMutI CAGTG 1 cut(s) 774
Cac8I GCNNGC 4 cut(s) 216, 320, 546, 678
CfoI GCGC 2 cut(s) 406, 818
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 4 cut(s) 189, 376, 463, 623
CseI GACGC 2 cut(s) 302, 398
Csp6I GTAC 8 cut(s) 653, 699, 996, 1011, 1245, 1483, 1683, 1693
CspAI ACCGGT 1 cut(s) 451
CviAII CATG 4 cut(s) 289, 656, 1585, 1613
CviQI GTAC 8 cut(s) 653, 699, 996, 1011, 1245, 1483, 1683, 1693
DdeI CTNAG 5 cut(s) 193, 407, 924, 1079, 1360
DpnI GATC 8 cut(s) 222, 347, 801, 872, 1021, 1053, 1059, 1140
DpnII GATC 8 cut(s) 220, 345, 799, 870, 1019, 1051, 1057, 1138
DraI TTTAAA 1 cut(s) 1068
DrdI GACNNNNNNGTC 1 cut(s) 1201
DriI GACNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 1201
Eam1104I CTCTTC 2 cut(s) 413, 1586
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 2 cut(s) 413, 1586
EciI GGCGGA 2 cut(s) 40, 49
Eco130I CCWWGG 3 cut(s) 288, 626, 729
Eco147I AGGCCT 1 cut(s) 371
Eco32I GATATC 1 cut(s) 265
Eco47I GGWCC 2 cut(s) 463, 623
Eco72I CACGTG 1 cut(s) 1285
Eco88I CYCGRG 1 cut(s) 689
EcoO109I RGGNCCY 1 cut(s) 623
EcoRII CCWGG 3 cut(s) 371, 478, 1434
EcoRV GATATC 1 cut(s) 265
EcoT14I CCWWGG 3 cut(s) 288, 626, 729
EcoT22I ATGCAT 2 cut(s) 364, 1330
ErhI CCWWGG 3 cut(s) 288, 626, 729
Esp3I CGTCTC 2 cut(s) 80, 304
FaeI CATG 4 cut(s) 292, 659, 1588, 1616
FaqI GGGAC 1 cut(s) 636
FatI CATG 4 cut(s) 288, 655, 1584, 1612
FauNDI CATATG 1 cut(s) 804
FbaI TGATCA 2 cut(s) 870, 1051
Fnu4HI GCNGC 7 cut(s) 379, 499, 596, 599, 760, 1404, 1551
FokI GGATG 7 cut(s) 185, 323, 553, 712, 775, 1332, 1517
Fsp4HI GCNGC 7 cut(s) 379, 499, 596, 599, 760, 1404, 1551
FspBI CTAG 4 cut(s) 233, 545, 858, 1416
GlaI GCGC 2 cut(s) 405, 817
GluI GCNGC 7 cut(s) 379, 499, 596, 599, 760, 1404, 1551
GsuI CTGGAG 1 cut(s) 1173
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 4 cut(s) 5, 191, 371, 378
HapII CCGG 6 cut(s) 63, 187, 315, 349, 452, 715
HgaI GACGC 2 cut(s) 302, 398
HhaI GCGC 2 cut(s) 406, 818
Hin1I GRCGYC 2 cut(s) 294, 390
Hin1II CATG 4 cut(s) 292, 659, 1588, 1616
Hin6I GCGC 2 cut(s) 404, 816
HinP1I GCGC 2 cut(s) 404, 816
HindIII AAGCTT 1 cut(s) 1577
HpaII CCGG 6 cut(s) 63, 187, 315, 349, 452, 715
HphI GGTGA 2 cut(s) 163, 466
Hpy166II GTNNAC 5 cut(s) 667, 699, 1421, 1598, 1685
Hpy188I TCNGA 4 cut(s) 25, 85, 400, 1207
Hpy188III TCNNGA 8 cut(s) 340, 689, 691, 939, 1055, 1152, 1214, 1371
Hpy8I GTNNAC 5 cut(s) 667, 699, 1421, 1598, 1685
Hpy99I CGWCG 1 cut(s) 825
HpyAV CCTTC 5 cut(s) 119, 187, 234, 1285, 1544
HpyCH4III ACNGT 4 cut(s) 467, 652, 769, 1425
HpyCH4IV ACGT 4 cut(s) 1107, 1284, 1481, 1687
HpyF10VI GCNNNNNNNGC 9 cut(s) 59, 245, 368, 554, 673, 756, 971, 1547, 1585
HpyF3I CTNAG 5 cut(s) 193, 407, 924, 1079, 1360
HpySE526I ACGT 4 cut(s) 1107, 1284, 1481, 1687
Hsp92I GRCGYC 2 cut(s) 294, 390
Hsp92II CATG 4 cut(s) 292, 659, 1588, 1616
HspAI GCGC 2 cut(s) 404, 816
Ksp22I TGATCA 2 cut(s) 870, 1051
Kzo9I GATC 8 cut(s) 220, 345, 799, 870, 1019, 1051, 1057, 1138
LguI GCTCTTC 1 cut(s) 413
LmnI GCTCC 4 cut(s) 718, 734, 1400, 1705
Lsp1109I GCAGC 4 cut(s) 485, 585, 1390, 1537
LweI GCATC 3 cut(s) 226, 531, 1539
MaeI CTAG 4 cut(s) 233, 545, 858, 1416
MaeII ACGT 4 cut(s) 1107, 1284, 1481, 1687
MaeIII GTNAC 2 cut(s) 875, 1280
MalI GATC 8 cut(s) 222, 347, 801, 872, 1021, 1053, 1059, 1140
MbiI CCGCTC 1 cut(s) 595
MboI GATC 8 cut(s) 220, 345, 799, 870, 1019, 1051, 1057, 1138
MboII GAAGA 5 cut(s) 133, 430, 1140, 1301, 1603
MflI RGATCY 4 cut(s) 345, 1019, 1057, 1138
MhlI GDGCHC 2 cut(s) 669, 1600
MlyI GAGTC 4 cut(s) 278, 404, 893, 1288
MmeI TCCRAC 1 cut(s) 108
Mph1103I ATGCAT 2 cut(s) 364, 1330
MroXI GAANNNNTTC 3 cut(s) 422, 829, 1632
MseI TTAA 6 cut(s) 563, 1037, 1067, 1323, 1518, 1581
MslI CAYNNNNRTG 3 cut(s) 767, 886, 1659
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 6 cut(s) 63, 187, 315, 349, 452, 715
MspR9I CCNGG 6 cut(s) 64, 188, 373, 480, 716, 1436
Mva1269I GAATGC 2 cut(s) 362, 920
MvaI CCWGG 3 cut(s) 373, 480, 1436
MwoI GCNNNNNNNGC 9 cut(s) 59, 245, 368, 554, 673, 756, 971, 1547, 1585
NciI CCSGG 3 cut(s) 64, 188, 716
NcoI CCATGG 1 cut(s) 288
NdeI CATATG 1 cut(s) 804
NdeII GATC 8 cut(s) 220, 345, 799, 870, 1019, 1051, 1057, 1138
NheI GCTAGC 1 cut(s) 544
NlaIII CATG 4 cut(s) 292, 659, 1588, 1616
NlaIV GGNNCC 4 cut(s) 347, 377, 624, 1059
NmeAIII GCCGAG 2 cut(s) 418, 607
NmuCI GTSAC 1 cut(s) 1280
NsiI ATGCAT 2 cut(s) 364, 1330
NspI RCATGY 1 cut(s) 1588
PaeR7I CTCGAG 1 cut(s) 689
PceI AGGCCT 1 cut(s) 371
PciSI GCTCTTC 1 cut(s) 413
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 396
PctI GAATGC 2 cut(s) 362, 920
PdmI GAANNNNTTC 3 cut(s) 422, 829, 1632
PfeI GAWTC 7 cut(s) 47, 56, 67, 1075, 1217, 1295, 1633
PflFI GACNNNGTC 1 cut(s) 296
PflMI CCANNNNNTGG 1 cut(s) 1629
PfoI TCCNGGA 1 cut(s) 714
PinAI ACCGGT 1 cut(s) 451
PkrI GCNGC 7 cut(s) 380, 500, 597, 600, 761, 1405, 1552
PleI GAGTC 4 cut(s) 278, 403, 893, 1287
PmaCI CACGTG 1 cut(s) 1285
PmlI CACGTG 1 cut(s) 1285
PpsI GAGTC 4 cut(s) 278, 403, 893, 1287
Ppu21I YACGTR 1 cut(s) 1285
PpuMI RGGWCCY 1 cut(s) 623
PshBI ATTAAT 1 cut(s) 1037
Psp5II RGGWCCY 1 cut(s) 623
Psp6I CCWGG 3 cut(s) 371, 478, 1434
PspCI CACGTG 1 cut(s) 1285
PspGI CCWGG 3 cut(s) 371, 478, 1434
PspN4I GGNNCC 4 cut(s) 347, 377, 624, 1059
PspPI GGNCC 4 cut(s) 189, 376, 463, 623
PspPPI RGGWCCY 1 cut(s) 623
PsrI GAACNNNNNNTAC 2 cut(s) 979, 1011
PsuI RGATCY 4 cut(s) 345, 1019, 1057, 1138
PsyI GACNNNGTC 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 8 cut(s) 654, 700, 997, 1012, 1246, 1484, 1684, 1694
RsaNI GTAC 8 cut(s) 653, 699, 996, 1011, 1245, 1483, 1683, 1693
RseI CAYNNNNRTG 3 cut(s) 767, 886, 1659
SapI GCTCTTC 1 cut(s) 413
SaqAI TTAA 6 cut(s) 563, 1037, 1067, 1323, 1518, 1581
SatI GCNGC 7 cut(s) 379, 499, 596, 599, 760, 1404, 1551
Sau3AI GATC 8 cut(s) 220, 345, 799, 870, 1019, 1051, 1057, 1138
Sau96I GGNCC 4 cut(s) 189, 376, 463, 623
SchI GAGTC 4 cut(s) 278, 404, 893, 1288
ScrFI CCNGG 6 cut(s) 64, 188, 373, 480, 716, 1436
SduI GDGCHC 2 cut(s) 669, 1600
SfaNI GCATC 3 cut(s) 226, 531, 1539
Sfr274I CTCGAG 1 cut(s) 689
SinI GGWCC 2 cut(s) 463, 623
SlaI CTCGAG 1 cut(s) 689
SmiMI CAYNNNNRTG 3 cut(s) 767, 886, 1659
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
SpeI ACTAGT 1 cut(s) 857
SseBI AGGCCT 1 cut(s) 371
SsiI CCGC 7 cut(s) 9, 51, 60, 271, 379, 595, 760
SspI AATATT 1 cut(s) 935
SspMI CTAG 4 cut(s) 233, 545, 858, 1416
StuI AGGCCT 1 cut(s) 371
StyD4I CCNGG 6 cut(s) 62, 186, 371, 478, 714, 1434
StyI CCWWGG 3 cut(s) 288, 626, 729
TaaI ACNGT 4 cut(s) 467, 652, 769, 1425
TaiI ACGT 4 cut(s) 1110, 1287, 1484, 1690
TaqI TCGA 9 cut(s) 45, 261, 282, 366, 486, 690, 823, 930, 985
TatI WGTACW 4 cut(s) 652, 698, 995, 1692
TauI GCSGC 3 cut(s) 381, 598, 762
TfiI GAWTC 7 cut(s) 47, 56, 67, 1075, 1217, 1295, 1633
Tru1I TTAA 6 cut(s) 563, 1037, 1067, 1323, 1518, 1581
Tru9I TTAA 6 cut(s) 563, 1037, 1067, 1323, 1518, 1581
TscAI CASTG 1 cut(s) 774
TseFI GTSAC 1 cut(s) 1280
TseI GCWGC 4 cut(s) 498, 598, 1403, 1550
Tsp45I GTSAC 1 cut(s) 1280
TspDTI ATGAA 6 cut(s) 59, 660, 834, 927, 1209, 1436
TspRI CASTG 1 cut(s) 774
Tth111I GACNNNGTC 1 cut(s) 296
Van91I CCANNNNNTGG 1 cut(s) 1629
VneI GTGCAC 2 cut(s) 665, 1596
VpaK11BI GGWCC 2 cut(s) 463, 623
VspI ATTAAT 1 cut(s) 1037
XapI RAATTY 4 cut(s) 167, 242, 457, 1069
XceI RCATGY 1 cut(s) 1588
XhoI CTCGAG 1 cut(s) 689
XmnI GAANNNNTTC 3 cut(s) 422, 829, 1632
XspI CTAG 4 cut(s) 233, 545, 858, 1416
Zsp2I ATGCAT 2 cut(s) 364, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.