FvH4_5g29931

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000800
Physical Location & Seq
Reverse (-)
20804544 .. 20807405
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29931.t1

Sequence Viewer

Length: 630 bp
ATGTTCCAGTGGGGACGTCGAATATGTTCAGCTTTATTTTCATTACAAGAGACTAGTTCGTCACTGACCAGTAACACATTGTTATGCTTGTTGGACTCCCTTGTTGATGAATGCAAGGCTGAGGTTGAATTTCCAGGAAAAGAGTTTGCTGAACTTATTGCTTATTGTGCCGATAATTGTCGTACTACAAGTACAAATCTTTCTGGTACTTTGAGATCGCATTTAAAAAAGTTAATAACTCACTTTGATCAGGTTCAACCTTTGAATTTGATTCTAAGCCTTTATGCTACTGGTTTGCACTACGTTGATTGTAGTTTAAAGTCAAAGGATGATGATCTTACATCTTCTGGGGGTGCAATGAGAATGTTTCTTGATGATAGGGGTATAACGACTGATTTGTCCAATTGTCTCGATTCGTTAGAAAGTTATTTCCAAATTGGTACAAATGATATTTCCAAAAGGGGGTTTTCCTTGAGTCACGTGCAGAAGAATTTAAAGGATTATCTTCTGCCTTATTATAATGCATTGGAGTTCTTATGCCTTCCACTTATTGACATAGTTTATTCGGGAAAGAAGCAGATCATTGCTGATAATGGAGCTGCCTTTGTTTCTAATGGAATGCGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

210

Amino Acids

23.28

Weight (kDa)

5.33

Isoelectric Point (pI)

26.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1_2nd PF25113 20 - 194 1.5e-36 Separase-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
AasI GACNNNNNNGTC 2 cut(s) 58, 397
AatII GACGTC 1 cut(s) 19
AciI CCGC 1 cut(s) 622
AcsI RAATTY 3 cut(s) 128, 265, 490
AcvI CACGTG 1 cut(s) 481
AcyI GRCGYC 1 cut(s) 16
AfaI GTAC 4 cut(s) 184, 193, 208, 442
AfiI CCNNNNNNNGG 1 cut(s) 462
AgsI TTSAA 3 cut(s) 128, 257, 265
AhlI ACTAGT 1 cut(s) 53
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 2 cut(s) 32, 599
AluI AGCT 2 cut(s) 32, 599
Alw26I GTCTC 2 cut(s) 44, 413
ApeKI GCWGC 1 cut(s) 599
ApoI RAATTY 3 cut(s) 128, 265, 490
Asp700I GAANNNNTTC 1 cut(s) 25
BbrPI CACGTG 1 cut(s) 481
BbvCI CCTCAGC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 586
BciT130I CCWGG 1 cut(s) 135
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 2 cut(s) 44, 413
BcuI ACTAGT 1 cut(s) 53
BfaI CTAG 1 cut(s) 54
BisI GCNGC 1 cut(s) 600
BlsI GCNGC 1 cut(s) 601
Bme1390I CCNGG 1 cut(s) 135
BmrFI CCNGG 1 cut(s) 135
Bpu10I CCTNAGC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 493
BsaAI YACGTR 1 cut(s) 481
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 16
Bsc4I CCNNNNNNNGG 1 cut(s) 462
Bse1I ACTGG 3 cut(s) 7, 69, 295
Bse3DI GCAATG 2 cut(s) 363, 582
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 1 cut(s) 135
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 462
BseMI GCAATG 2 cut(s) 363, 582
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 3 cut(s) 7, 69, 295
BseXI GCAGC 1 cut(s) 586
BsgI GTGCAG 1 cut(s) 503
BslFI GGGAC 1 cut(s) 27
BslI CCNNNNNNNGG 1 cut(s) 462
BsmAI GTCTC 2 cut(s) 44, 413
BsmFI GGGAC 1 cut(s) 27
BsmI GAATGC 2 cut(s) 116, 624
Bsp143I GATC 4 cut(s) 215, 247, 334, 579
BspACI CCGC 1 cut(s) 622
BspCNI CTCAG 1 cut(s) 112
BsrDI GCAATG 2 cut(s) 363, 582
BsrI ACTGG 3 cut(s) 7, 69, 295
BssMI GATC 4 cut(s) 215, 247, 334, 579
BssNI GRCGYC 1 cut(s) 16
Bst2UI CCWGG 1 cut(s) 135
BstACI GRCGYC 1 cut(s) 16
BstBAI YACGTR 1 cut(s) 481
BstDEI CTNAG 2 cut(s) 120, 275
BstF5I GGATG 1 cut(s) 334
BstKTI GATC 4 cut(s) 218, 250, 337, 582
BstMAI GTCTC 2 cut(s) 44, 413
BstMBI GATC 4 cut(s) 215, 247, 334, 579
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstV1I GCAGC 1 cut(s) 586
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 2 cut(s) 14, 62
Csp6I GTAC 4 cut(s) 183, 192, 207, 441
CviJI RGCY 4 cut(s) 32, 119, 279, 599
CviKI_1 RGCY 4 cut(s) 32, 119, 279, 599
CviQI GTAC 4 cut(s) 183, 192, 207, 441
DdeI CTNAG 2 cut(s) 120, 275
DpnI GATC 4 cut(s) 217, 249, 336, 581
DpnII GATC 4 cut(s) 215, 247, 334, 579
DraI TTTAAA 3 cut(s) 225, 318, 495
DrdI GACNNNNNNGTC 2 cut(s) 58, 397
DseDI GACNNNNNNGTC 2 cut(s) 58, 397
Eco72I CACGTG 1 cut(s) 481
EcoRII CCWGG 1 cut(s) 133
EcoT22I ATGCAT 1 cut(s) 526
FaiI YATR 7 cut(s) 25, 85, 285, 386, 519, 538, 557
FaqI GGGAC 1 cut(s) 27
FauI CCCGC 1 cut(s) 615
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 1 cut(s) 600
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 1 cut(s) 600
FspBI CTAG 1 cut(s) 54
GluI GCNGC 1 cut(s) 600
Hin1I GRCGYC 1 cut(s) 16
HinfI GANTC 4 cut(s) 95, 271, 413, 475
Hpy188III TCNNGA 3 cut(s) 371, 410, 567
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 1 cut(s) 551
HpyCH4IV ACGT 3 cut(s) 16, 303, 480
HpyCH4V TGCA 5 cut(s) 114, 298, 356, 484, 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 2 cut(s) 120, 275
HpySE526I ACGT 3 cut(s) 16, 303, 480
Hsp92I GRCGYC 1 cut(s) 16
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 4 cut(s) 215, 247, 334, 579
LmnI GCTCC 1 cut(s) 596
LpnPI CCDG 8 cut(s) 20, 82, 120, 147, 189, 236, 276, 333
Lsp1109I GCAGC 1 cut(s) 586
MaeI CTAG 1 cut(s) 54
MaeII ACGT 3 cut(s) 16, 303, 480
MaeIII GTNAC 3 cut(s) 60, 71, 476
MalI GATC 4 cut(s) 217, 249, 336, 581
MboI GATC 4 cut(s) 215, 247, 334, 579
MboII GAAGA 3 cut(s) 336, 497, 499
MfeI CAATTG 1 cut(s) 403
MluCI AATT 6 cut(s) 128, 175, 265, 403, 435, 490
MlyI GAGTC 2 cut(s) 89, 484
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 1 cut(s) 115
Mph1103I ATGCAT 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 25
MseI TTAA 5 cut(s) 224, 233, 317, 494, 628
MslI CAYNNNNRTG 1 cut(s) 82
MspR9I CCNGG 1 cut(s) 135
MunI CAATTG 1 cut(s) 403
Mva1269I GAATGC 2 cut(s) 116, 624
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 1 cut(s) 167
NdeII GATC 4 cut(s) 215, 247, 334, 579
NmuCI GTSAC 2 cut(s) 60, 476
NsiI ATGCAT 1 cut(s) 526
PctI GAATGC 2 cut(s) 116, 624
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 2 cut(s) 271, 413
PfoI TCCNGGA 1 cut(s) 133
PkrI GCNGC 1 cut(s) 601
PleI GAGTC 2 cut(s) 89, 483
PmaCI CACGTG 1 cut(s) 481
PmlI CACGTG 1 cut(s) 481
PpsI GAGTC 2 cut(s) 89, 483
Ppu21I YACGTR 1 cut(s) 481
PsiI TTATAA 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 133
PspCI CACGTG 1 cut(s) 481
PspGI CCWGG 1 cut(s) 133
RsaI GTAC 4 cut(s) 184, 193, 208, 442
RsaNI GTAC 4 cut(s) 183, 192, 207, 441
RseI CAYNNNNRTG 1 cut(s) 82
SaqAI TTAA 5 cut(s) 224, 233, 317, 494, 628
SatI GCNGC 1 cut(s) 600
Sau3AI GATC 4 cut(s) 215, 247, 334, 579
SchI GAGTC 2 cut(s) 89, 484
ScrFI CCNGG 1 cut(s) 135
SetI ASST 8 cut(s) 19, 34, 126, 255, 262, 306, 483, 601
SmiMI CAYNNNNRTG 1 cut(s) 82
SmlI CTYRAG 1 cut(s) 472
SmoI CTYRAG 1 cut(s) 472
SpeI ACTAGT 1 cut(s) 53
Sse9I AATT 6 cut(s) 128, 175, 265, 403, 435, 490
SsiI CCGC 1 cut(s) 622
SspMI CTAG 1 cut(s) 54
StyD4I CCNGG 1 cut(s) 133
TaiI ACGT 3 cut(s) 19, 306, 483
TaqI TCGA 2 cut(s) 19, 411
TasI AATT 6 cut(s) 128, 175, 265, 403, 435, 490
TatI WGTACW 1 cut(s) 191
TfiI GAWTC 2 cut(s) 271, 413
Tru1I TTAA 5 cut(s) 224, 233, 317, 494, 628
Tru9I TTAA 5 cut(s) 224, 233, 317, 494, 628
TscAI CASTG 2 cut(s) 14, 69
TseFI GTSAC 2 cut(s) 60, 476
TseI GCWGC 1 cut(s) 599
Tsp45I GTSAC 2 cut(s) 60, 476
TspDTI ATGAA 2 cut(s) 30, 123
TspRI CASTG 2 cut(s) 14, 69
XapI RAATTY 3 cut(s) 128, 265, 490
XmnI GAANNNNTTC 1 cut(s) 25
XspI CTAG 1 cut(s) 54
ZraI GACGTC 1 cut(s) 17
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.