MD08G1226100.v1.1

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000800
Physical Location & Seq
Forward (+)
29272170 .. 29275542
3373 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1226100.v1.1.491

Sequence Viewer

Length: 1599 bp
ATGAAGGACAGAAAGAACTATCTTCTGTCTTATTTTAATGCATTGAAATTCTTATGCCAGCCGCTTACAGAGTTAGTAAATTCAGGAAGGAAACAGATTATTACAAATAATGAAGCTGCTTCTGTTTCTATTGAATTGTGCCATATCCAGGGTGCATTCCATCAGTTTTGTTTTCTTTCTCTGCAAATGTGCAGATGTACATATGAGGTGGATAGAGATGGTTTTGATGGAAACAGCACCCTTGATGTTGCTCTGGCTGCTTTTACCCTTGCCATCATAACAAAGCTGAACATGCAGAGTGTGCAAATACTTGAAAATATCATCACCAGGGCTTGGATTCAACCTCATGGTCTAAAGTATCTATATGTCTCTCTGTACAATACCGGGATACATTTGTACCGAAAGAAGGAGCTAAAAGAGGCTCTGATAGGGATGCAGTTGTTTACGTGCACTTCTCCTTGCCATCAATTAGCTGTGGCATATTGCTTACATGCGCTTTCTACCCAGGAAACTGAACCCAACTCAAAGACTCATGAGCAGCGAGTGCTTGAAGATATCAGTGCTGCCATTAATCTATGGTTGGGCATTTCTACTCCAGATAATTGCTCTCCTGCTGACAAGTGGTCCATGTTGTCTGAAAATACCATATTGCTACTGTACAATGTTATTGATCTGTTATCCATCAAGGGTTGCACGGACTTTCACAGTGATATACATAGGCTTATGATTAGATTATTTAAATGGAGTAATGTCCCATTAGAGAAGTGCGTCCTCAGATTTTGGGAATGTAGAAGGATTAGCCATGCCCTTTGTGCTTCACCTGTAAATGAGACATTCATCATGAACATATCGGATCATTGTGGTGAACTTTCAAAGTCTATTTCTTTTTGGATAGATTGTCTAAAAGATTCCAAACAATTGCTTTTAGCGTTCCAACATAGCTTCTCATTTCTGTTTCCAAACTTTTCTCGGGGCCCATGCAATCATGAAAGCTTGTTTCGATCAGATACTGAGGTCAAGCAAGCTGCTTTTGAACTTATTTCACAAGCTCCTGTATCTACTTGGTCTGCTTACGTAGCTGGATATCTCTACTATGATTTATCTGAAAGACTTGTTTCAAATGGACGGTTAATTGAGGCTCTCTCATATGCCAAAGAAGCTCACAACTTCTCACAAAGCTTACCACTATTTACCAATGTTTTTTCTACTGTCTTGGGAAAGCTGTACCATAAGCAGCAGCATTGGGATTTGGCACAAAAGGAACTTCAAAGTGCCAAGGAATATTTTGGGGATAGCAGCTCTGATATTTCTTGTTGGAAGTGCAGATTGATGCTGGAAGCAACTGTTAATCCTGTATTTTTGAAAATACAAGGAAGTACATCTTCAGATAAGTTATCTCATGCTGAAAACCTGTATAAATCAGCCATTGCCAAACTAAATCTTTCTGAGTGGAAAATTCTGTTAATTGTCCTGAAGAAGAGCGTGATGATTGGACAATGCTTGGAAAAGCTAGTCTTAAAGACTTTGGAGCTTGTTGTATACTTACTCATTCTGAAGAAAAACAGCAGGAAATTAGGAAAATCATCAAGGAGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

533

Amino Acids

60.63

Weight (kDa)

8.57

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1_2nd PF25113 2 - 33 1.2e-09 Separase-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 333
AccI GTMKAC 1 cut(s) 1539
AciI CCGC 1 cut(s) 62
AclWI GGATC 1 cut(s) 861
AcsI RAATTY 3 cut(s) 47, 79, 1455
AcuI CTGAAG 3 cut(s) 1368, 1493, 1574
AfaI GTAC 6 cut(s) 199, 377, 398, 659, 1226, 1378
AfiI CCNNNNNNNGG 3 cut(s) 148, 333, 406
AhdI GACNNNNNGTC 1 cut(s) 622
AjnI CCWGG 3 cut(s) 147, 326, 504
Alw21I GWGCWC 1 cut(s) 452
Alw26I GTCTC 2 cut(s) 373, 824
Alw44I GTGCAC 1 cut(s) 448
AlwI GGATC 1 cut(s) 861
AlwNI CAGNNNCTG 1 cut(s) 1010
Ama87I CYCGRG 1 cut(s) 969
AoxI GGCC 1 cut(s) 973
ApaI GGGCCC 1 cut(s) 977
ApaLI GTGCAC 1 cut(s) 448
ApeKI GCWGC 8 cut(s) 116, 257, 538, 563, 1025, 1234, 1237, 1296
ApoI RAATTY 3 cut(s) 47, 79, 1455
AseI ATTAAT 1 cut(s) 570
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 3 cut(s) 624, 973, 974
AsuC2I CCSGG 1 cut(s) 385
AsuHPI GGTGA 3 cut(s) 316, 810, 875
AvaI CYCGRG 1 cut(s) 969
AvaII GGWCC 1 cut(s) 624
BaeGI GKGCMC 2 cut(s) 452, 977
BaeI ACNNNNGTAYC 6 cut(s) 380, 380, 413, 413, 999, 1032
BanII GRGCYC 1 cut(s) 977
Bbv12I GWGCWC 1 cut(s) 452
BbvI GCAGC 8 cut(s) 103, 244, 550, 550, 1012, 1246, 1249, 1308
BccI CCATC 6 cut(s) 168, 212, 221, 281, 471, 689
BciT130I CCWGG 3 cut(s) 149, 328, 506
BciVI GTATCC 1 cut(s) 381
BcnI CCSGG 1 cut(s) 385
BcoDI GTCTC 2 cut(s) 373, 824
BfaI CTAG 1 cut(s) 1511
BfuI GTATCC 1 cut(s) 381
BisI GCNGC 9 cut(s) 62, 117, 258, 539, 564, 1026, 1235, 1238, 1297
BlsI GCNGC 9 cut(s) 63, 118, 259, 540, 565, 1027, 1236, 1239, 1298
Bme1390I CCNGG 4 cut(s) 149, 328, 385, 506
Bme18I GGWCC 1 cut(s) 624
BmeRI GACNNNNNGTC 1 cut(s) 622
BmeT110I CYCGRG 1 cut(s) 969
BmgT120I GGNCC 3 cut(s) 624, 973, 974
BmiI GGNNCC 2 cut(s) 974, 975
BmrFI CCNGG 4 cut(s) 149, 328, 385, 506
BmsI GCATC 2 cut(s) 423, 1320
BplI GAGNNNNNCTC 2 cut(s) 1127, 1159
BpmI CTGGAG 1 cut(s) 579
BpuMI CCSGG 1 cut(s) 385
BsaAI YACGTR 2 cut(s) 447, 1075
BsaJI CCNNGG 4 cut(s) 148, 327, 504, 1275
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 333, 406
Bse3DI GCAATG 1 cut(s) 1425
BseBI CCWGG 3 cut(s) 149, 328, 506
BseDI CCNNGG 4 cut(s) 148, 327, 504, 1275
BseGI GGATG 1 cut(s) 438
BseLI CCNNNNNNNGG 3 cut(s) 148, 333, 406
BseMI GCAATG 1 cut(s) 1425
BseMII CTCAG 3 cut(s) 787, 1002, 1437
BseSI GKGCMC 2 cut(s) 452, 977
BseXI GCAGC 8 cut(s) 103, 244, 550, 550, 1012, 1246, 1249, 1308
BsgI GTGCAG 2 cut(s) 211, 1342
BshFI GGCC 1 cut(s) 975
BsiHKAI GWGCWC 1 cut(s) 452
BsiHKCI CYCGRG 1 cut(s) 969
BsiSI CCGG 1 cut(s) 384
BslFI GGGAC 1 cut(s) 737
BslI CCNNNNNNNGG 3 cut(s) 148, 333, 406
BsmAI GTCTC 2 cut(s) 373, 824
BsmFI GGGAC 1 cut(s) 737
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 1 cut(s) 975
BsoBI CYCGRG 1 cut(s) 969
Bsp120I GGGCCC 1 cut(s) 973
Bsp1286I GDGCHC 2 cut(s) 452, 977
Bsp1407I TGTACA 3 cut(s) 197, 375, 657
Bsp143I GATC 3 cut(s) 670, 853, 1001
BspACI CCGC 1 cut(s) 62
BspANI GGCC 1 cut(s) 975
BspCNI CTCAG 3 cut(s) 786, 1003, 1438
BspHI TCATGA 3 cut(s) 532, 840, 985
BspLI GGNNCC 2 cut(s) 974, 975
BspPI GGATC 1 cut(s) 861
BspQI GCTCTTC 1 cut(s) 1472
BsrDI GCAATG 1 cut(s) 1425
BsrGI TGTACA 3 cut(s) 197, 375, 657
BssECI CCNNGG 4 cut(s) 148, 327, 504, 1275
BssMI GATC 3 cut(s) 670, 853, 1001
BssNAI GTATAC 1 cut(s) 1540
BssT1I CCWWGG 1 cut(s) 1275
Bst1107I GTATAC 1 cut(s) 1540
Bst2UI CCWGG 3 cut(s) 149, 328, 506
Bst4CI ACNGT 5 cut(s) 657, 707, 1128, 1210, 1345
Bst6I CTCTTC 1 cut(s) 1472
BstAPI GCANNNNNTGC 2 cut(s) 301, 544
BstAUI TGTACA 3 cut(s) 197, 375, 657
BstBAI YACGTR 2 cut(s) 447, 1075
BstC8I GCNNGC 2 cut(s) 59, 1023
BstDEI CTNAG 3 cut(s) 773, 1011, 1446
BstF5I GGATG 1 cut(s) 438
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 3 cut(s) 673, 856, 1004
BstMAI GTCTC 2 cut(s) 373, 824
BstMBI GATC 3 cut(s) 670, 853, 1001
BstMWI GCNNNNNNNGC 7 cut(s) 257, 292, 301, 544, 812, 1076, 1157
BstNI CCWGG 3 cut(s) 149, 328, 506
BstNSI RCATGY 2 cut(s) 295, 494
BstSCI CCNGG 4 cut(s) 147, 326, 383, 504
BstSLI GKGCMC 2 cut(s) 452, 977
BstSNI TACGTA 1 cut(s) 1075
BstV1I GCAGC 8 cut(s) 103, 244, 550, 550, 1012, 1246, 1249, 1308
BstZ17I GTATAC 1 cut(s) 1540
BsuI GTATCC 1 cut(s) 381
BsuRI GGCC 1 cut(s) 975
BtsCI GGATG 1 cut(s) 438
BtsIMutI CAGTG 2 cut(s) 565, 712
Cac8I GCNNGC 2 cut(s) 59, 1023
CaiI CAGNNNCTG 1 cut(s) 1010
CciI TCATGA 3 cut(s) 532, 840, 985
CfoI GCGC 1 cut(s) 496
Cfr13I GGNCC 3 cut(s) 624, 973, 974
CseI GACGC 1 cut(s) 757
Csp6I GTAC 6 cut(s) 198, 376, 397, 658, 1225, 1377
CviQI GTAC 6 cut(s) 198, 376, 397, 658, 1225, 1377
DdeI CTNAG 3 cut(s) 773, 1011, 1446
DpnI GATC 3 cut(s) 672, 855, 1003
DpnII GATC 3 cut(s) 670, 853, 1001
DraI TTTAAA 1 cut(s) 739
DriI GACNNNNNGTC 1 cut(s) 622
Eam1104I CTCTTC 1 cut(s) 1472
Eam1105I GACNNNNNGTC 1 cut(s) 622
EarI CTCTTC 1 cut(s) 1472
Eco105I TACGTA 1 cut(s) 1075
Eco130I CCWWGG 1 cut(s) 1275
Eco24I GRGCYC 1 cut(s) 977
Eco32I GATATC 2 cut(s) 556, 1085
Eco47I GGWCC 1 cut(s) 624
Eco57I CTGAAG 3 cut(s) 1368, 1493, 1574
Eco88I CYCGRG 1 cut(s) 969
EcoO109I RGGNCCY 1 cut(s) 973
EcoRII CCWGG 3 cut(s) 147, 326, 504
EcoRV GATATC 2 cut(s) 556, 1085
EcoT14I CCWWGG 1 cut(s) 1275
EcoT22I ATGCAT 1 cut(s) 43
EcoT38I GRGCYC 1 cut(s) 977
ErhI CCWWGG 1 cut(s) 1275
FalI AAGNNNNNCTT 4 cut(s) 1366, 1398, 1499, 1531
FaqI GGGAC 1 cut(s) 737
FauNDI CATATG 2 cut(s) 202, 1147
FblI GTMKAC 1 cut(s) 1539
Fnu4HI GCNGC 9 cut(s) 62, 117, 258, 539, 564, 1026, 1235, 1238, 1297
FokI GGATG 1 cut(s) 445
FriOI GRGCYC 1 cut(s) 977
Fsp4HI GCNGC 9 cut(s) 62, 117, 258, 539, 564, 1026, 1235, 1238, 1297
FspBI CTAG 1 cut(s) 1511
GlaI GCGC 1 cut(s) 495
GluI GCNGC 9 cut(s) 62, 117, 258, 539, 564, 1026, 1235, 1238, 1297
GsuI CTGGAG 1 cut(s) 579
HaeIII GGCC 1 cut(s) 975
HapII CCGG 1 cut(s) 384
HgaI GACGC 1 cut(s) 757
HhaI GCGC 1 cut(s) 496
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HindIII AAGCTT 2 cut(s) 991, 1177
HinfI GANTC 3 cut(s) 337, 529, 908
HpaII CCGG 1 cut(s) 384
HphI GGTGA 3 cut(s) 316, 810, 875
Hpy166II GTNNAC 4 cut(s) 444, 450, 866, 1540
Hpy188III TCNNGA 6 cut(s) 84, 533, 596, 841, 986, 1471
Hpy8I GTNNAC 4 cut(s) 444, 450, 866, 1540
HpyAV CCTTC 3 cut(s) 81, 400, 786
HpyCH4III ACNGT 5 cut(s) 657, 707, 1128, 1210, 1345
HpyCH4IV ACGT 2 cut(s) 446, 1074
HpyF10VI GCNNNNNNNGC 7 cut(s) 257, 292, 301, 544, 812, 1076, 1157
HpyF3I CTNAG 3 cut(s) 773, 1011, 1446
HpySE526I ACGT 2 cut(s) 446, 1074
HspAI GCGC 1 cut(s) 494
Kzo9I GATC 3 cut(s) 670, 853, 1001
LguI GCTCTTC 1 cut(s) 1472
LmnI GCTCC 3 cut(s) 409, 1054, 1528
Lsp1109I GCAGC 8 cut(s) 103, 244, 550, 550, 1012, 1246, 1249, 1308
LweI GCATC 2 cut(s) 423, 1320
MaeI CTAG 1 cut(s) 1511
MaeII ACGT 2 cut(s) 446, 1074
MalI GATC 3 cut(s) 672, 855, 1003
MboI GATC 3 cut(s) 670, 853, 1001
MboII GAAGA 6 cut(s) 14, 563, 1374, 1486, 1489, 1567
MfeI CAATTG 1 cut(s) 917
MhlI GDGCHC 2 cut(s) 452, 977
MlyI GAGTC 1 cut(s) 523
MmeI TCCRAC 2 cut(s) 958, 1295
MnlI CCTC 7 cut(s) 199, 354, 412, 782, 1006, 1129, 1584
Mph1103I ATGCAT 1 cut(s) 43
MseI TTAA 7 cut(s) 36, 570, 738, 1130, 1347, 1463, 1517
MslI CAYNNNNRTG 1 cut(s) 861
MspI CCGG 1 cut(s) 384
MspR9I CCNGG 4 cut(s) 149, 328, 385, 506
MunI CAATTG 1 cut(s) 917
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 3 cut(s) 149, 328, 506
MwoI GCNNNNNNNGC 7 cut(s) 257, 292, 301, 544, 812, 1076, 1157
NciI CCSGG 1 cut(s) 385
NdeI CATATG 2 cut(s) 202, 1147
NdeII GATC 3 cut(s) 670, 853, 1001
NlaIV GGNNCC 2 cut(s) 974, 975
NsiI ATGCAT 1 cut(s) 43
NspI RCATGY 2 cut(s) 295, 494
PagI TCATGA 3 cut(s) 532, 840, 985
PciSI GCTCTTC 1 cut(s) 1472
PctI GAATGC 1 cut(s) 155
PfeI GAWTC 2 cut(s) 337, 908
PflMI CCANNNNNTGG 1 cut(s) 333
PkrI GCNGC 9 cut(s) 63, 118, 259, 540, 565, 1027, 1236, 1239, 1298
PleI GAGTC 1 cut(s) 523
PpsI GAGTC 1 cut(s) 523
Ppu21I YACGTR 2 cut(s) 447, 1075
PshBI ATTAAT 1 cut(s) 570
Psp6I CCWGG 3 cut(s) 147, 326, 504
PspGI CCWGG 3 cut(s) 147, 326, 504
PspN4I GGNNCC 2 cut(s) 974, 975
PspOMI GGGCCC 1 cut(s) 973
PspPI GGNCC 3 cut(s) 624, 973, 974
PstNI CAGNNNCTG 1 cut(s) 1010
RsaI GTAC 6 cut(s) 199, 377, 398, 659, 1226, 1378
RsaNI GTAC 6 cut(s) 198, 376, 397, 658, 1225, 1377
RseI CAYNNNNRTG 1 cut(s) 861
SapI GCTCTTC 1 cut(s) 1472
SaqAI TTAA 7 cut(s) 36, 570, 738, 1130, 1347, 1463, 1517
SatI GCNGC 9 cut(s) 62, 117, 258, 539, 564, 1026, 1235, 1238, 1297
Sau3AI GATC 3 cut(s) 670, 853, 1001
Sau96I GGNCC 3 cut(s) 624, 973, 974
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 4 cut(s) 149, 328, 385, 506
SduI GDGCHC 2 cut(s) 452, 977
SfaNI GCATC 2 cut(s) 423, 1320
SinI GGWCC 1 cut(s) 624
SmiI ATTTAAAT 1 cut(s) 739
SmiMI CAYNNNNRTG 1 cut(s) 861
SnaBI TACGTA 1 cut(s) 1075
SsiI CCGC 1 cut(s) 62
SspI AATATT 1 cut(s) 1283
SspMI CTAG 1 cut(s) 1511
StyD4I CCNGG 4 cut(s) 147, 326, 383, 504
StyI CCWWGG 1 cut(s) 1275
SwaI ATTTAAAT 1 cut(s) 739
TaaI ACNGT 5 cut(s) 657, 707, 1128, 1210, 1345
TaiI ACGT 2 cut(s) 449, 1077
TaqI TCGA 1 cut(s) 1000
TatI WGTACW 4 cut(s) 197, 375, 657, 1376
TauI GCSGC 1 cut(s) 64
TfiI GAWTC 2 cut(s) 337, 908
Tru1I TTAA 7 cut(s) 36, 570, 738, 1130, 1347, 1463, 1517
Tru9I TTAA 7 cut(s) 36, 570, 738, 1130, 1347, 1463, 1517
TscAI CASTG 2 cut(s) 565, 712
TseI GCWGC 8 cut(s) 116, 257, 538, 563, 1025, 1234, 1237, 1296
TspDTI ATGAA 5 cut(s) 17, 126, 826, 857, 1002
TspGWI ACGGA 1 cut(s) 710
TspRI CASTG 2 cut(s) 565, 712
Van91I CCANNNNNTGG 1 cut(s) 333
VneI GTGCAC 1 cut(s) 448
VpaK11BI GGWCC 1 cut(s) 624
VspI ATTAAT 1 cut(s) 570
XapI RAATTY 3 cut(s) 47, 79, 1455
XceI RCATGY 2 cut(s) 295, 494
XmiI GTMKAC 1 cut(s) 1539
XspI CTAG 1 cut(s) 1511
Zsp2I ATGCAT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.