RLG00000001950

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000800
Physical Location & Seq
Forward (+)
23377935 .. 23380134
2200 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001950

Sequence Viewer

Length: 807 bp
ATGCTTCTACCAGTGGATTCACTATTAGAAGGAGATTCAGATGATGTTAGTTATTCTGGAATAAAGGATTGGAACTGTCCATGGGGTTCCACTGTGGTTGATGATGTAGCTCCAGAGTTTAGATTGATATTGGAGGGGGCACATTCGTCATCTGTGAAACATCCTGTACAAGATACAGATGAGAAAAAGCTTCACCGGTGGGTGGAAAGGAAGAATTTTGATCGCCATGCACTTGGAGGTGCACGTGTTACACAGCTTTGTCTTAAGAGAGGTACCTTTGTTAGTAAAGTTCGATGTTTAGAGGAAGAGAAGTGTATGGCATCACGTAGTGAATCTAATGGTGGCGAGAACTTGTCTGAGTTGGCCAACAAACTAGTATTAGAAGCAGTGAATGAGCTTGAAGGTTTACATTCTTATGTAAATATAGAGCCAACAATATTAGTTCTGGACTACGAGGTGCAGGGGAAGGCTGGATGTGCACCTCCTGCTGAAGAATTGGCAGTAGCGTTGAAAAGCCATGATCCATTTATGTACATTGGCCATGGATGTGGTATGTTTCGATTTTACATATTGCTTTGTGTTGCTGTATCTATACAGGCAACAATAATACAATATATTCCTAGACTTGAGATTCAGAGACTGGAACACTGTGCCTCAACTCTCCTGATGGGATGCAGTAGTGGTTCTCTGAAACACTATGGATGTTATGTTCCACAAGGTACTCCACTATCCTATCTGCTGGCTGGTTCTCCTGTCATTCTCCTGATATTAACCGATTTGCAAATTCCATGCTTGATTCTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

269

Amino Acids

29.5

Weight (kDa)

5.37

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Separin_C PF03568 204 - 257 3.5e-17 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 272
AclWI GGATC 1 cut(s) 515
AcoI YGGCCR 2 cut(s) 363, 538
AcsI RAATTY 2 cut(s) 214, 783
AcuI CTGAAG 1 cut(s) 510
AcvI CACGTG 1 cut(s) 245
AdeI CACNNNGTG 1 cut(s) 329
AfaI GTAC 4 cut(s) 168, 274, 533, 721
AfiI CCNNNNNNNGG 1 cut(s) 202
AflII CTTAAG 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 244
AgeI ACCGGT 1 cut(s) 195
AgsI TTSAA 2 cut(s) 401, 511
AhlI ACTAGT 1 cut(s) 373
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
AloI GAACNNNNNNTCC 2 cut(s) 693, 725
AluBI AGCT 4 cut(s) 110, 190, 256, 397
AluI AGCT 4 cut(s) 110, 190, 256, 397
Alw21I GWGCWC 2 cut(s) 244, 481
Alw26I GTCTC 1 cut(s) 631
Alw44I GTGCAC 2 cut(s) 240, 477
AlwI GGATC 1 cut(s) 515
AlwNI CAGNNNCTG 1 cut(s) 640
AoxI GGCC 2 cut(s) 363, 538
ApaLI GTGCAC 2 cut(s) 240, 477
ApoI RAATTY 2 cut(s) 214, 783
AsiGI ACCGGT 1 cut(s) 195
Asp718I GGTACC 1 cut(s) 272
AsuHPI GGTGA 1 cut(s) 185
BaeGI GKGCMC 3 cut(s) 142, 244, 481
BaeI ACNNNNGTAYC 2 cut(s) 264, 297
BalI TGGCCA 2 cut(s) 365, 540
BanI GGYRCC 1 cut(s) 272
BbrPI CACGTG 1 cut(s) 245
Bbv12I GWGCWC 2 cut(s) 244, 481
BccI CCATC 1 cut(s) 661
BcoDI GTCTC 1 cut(s) 631
BcuI ACTAGT 1 cut(s) 373
BfaI CTAG 2 cut(s) 374, 621
BfrI CTTAAG 1 cut(s) 263
BmiI GGNNCC 2 cut(s) 88, 274
BmsI GCATC 2 cut(s) 329, 662
BpmI CTGGAG 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 647
BsaAI YACGTR 2 cut(s) 245, 326
BsaJI CCNNGG 2 cut(s) 80, 541
BsaWI WCCGGW 1 cut(s) 195
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse118I RCCGGY 1 cut(s) 195
Bse1I ACTGG 2 cut(s) 11, 645
BseDI CCNNGG 2 cut(s) 80, 541
BseGI GGATG 5 cut(s) 160, 479, 551, 677, 707
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 2 cut(s) 11, 645
BseSI GKGCMC 3 cut(s) 142, 244, 481
BsgI GTGCAG 1 cut(s) 479
BshFI GGCC 2 cut(s) 365, 540
BshNI GGYRCC 1 cut(s) 272
BshTI ACCGGT 1 cut(s) 195
BsiHKAI GWGCWC 2 cut(s) 244, 481
BsiSI CCGG 1 cut(s) 196
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 631
BsnI GGCC 2 cut(s) 365, 540
Bsp1286I GDGCHC 3 cut(s) 142, 244, 481
Bsp1407I TGTACA 2 cut(s) 166, 531
Bsp143I GATC 2 cut(s) 220, 520
Bsp19I CCATGG 2 cut(s) 80, 541
BspANI GGCC 2 cut(s) 365, 540
BspCNI CTCAG 1 cut(s) 349
BspLI GGNNCC 2 cut(s) 88, 274
BspPI GGATC 1 cut(s) 515
BspT107I GGYRCC 1 cut(s) 272
BspTI CTTAAG 1 cut(s) 263
BsrFI RCCGGY 1 cut(s) 195
BsrGI TGTACA 2 cut(s) 166, 531
BsrI ACTGG 2 cut(s) 11, 645
BssAI RCCGGY 1 cut(s) 195
BssECI CCNNGG 2 cut(s) 80, 541
BssMI GATC 2 cut(s) 220, 520
BssT1I CCWWGG 2 cut(s) 80, 541
Bst4CI ACNGT 3 cut(s) 77, 94, 650
Bst6I CTCTTC 1 cut(s) 300
BstAFI CTTAAG 1 cut(s) 263
BstAPI GCANNNNNTGC 1 cut(s) 485
BstAUI TGTACA 2 cut(s) 166, 531
BstBAI YACGTR 2 cut(s) 245, 326
BstC8I GCNNGC 1 cut(s) 741
BstDEI CTNAG 1 cut(s) 357
BstDSI CCRYGG 2 cut(s) 80, 541
BstF5I GGATG 5 cut(s) 160, 479, 551, 677, 707
BstKTI GATC 2 cut(s) 223, 523
BstMAI GTCTC 1 cut(s) 631
BstMBI GATC 2 cut(s) 220, 520
BstMWI GCNNNNNNNGC 2 cut(s) 476, 485
BstSLI GKGCMC 3 cut(s) 142, 244, 481
BstXI CCANNNNNNTGG 2 cut(s) 233, 548
BsuRI GGCC 2 cut(s) 365, 540
BtgI CCRYGG 2 cut(s) 80, 541
BtsCI GGATG 5 cut(s) 160, 479, 551, 677, 707
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 4 cut(s) 18, 90, 393, 646
Cac8I GCNNGC 1 cut(s) 741
CaiI CAGNNNCTG 1 cut(s) 640
Cfr10I RCCGGY 1 cut(s) 195
Csp6I GTAC 4 cut(s) 167, 273, 532, 720
CspAI ACCGGT 1 cut(s) 195
CspCI CAANNNNNGTGG 2 cut(s) 79, 114
CviAII CATG 5 cut(s) 81, 227, 518, 542, 789
CviQI GTAC 4 cut(s) 167, 273, 532, 720
DdeI CTNAG 1 cut(s) 357
DpnI GATC 2 cut(s) 222, 522
DpnII GATC 2 cut(s) 220, 520
DraIII CACNNNGTG 1 cut(s) 329
EaeI YGGCCR 2 cut(s) 363, 538
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco130I CCWWGG 2 cut(s) 80, 541
Eco57I CTGAAG 1 cut(s) 510
Eco72I CACGTG 1 cut(s) 245
EcoT14I CCWWGG 2 cut(s) 80, 541
ErhI CCWWGG 2 cut(s) 80, 541
FaeI CATG 5 cut(s) 84, 230, 521, 545, 792
FatI CATG 5 cut(s) 80, 226, 517, 541, 788
FokI GGATG 5 cut(s) 147, 486, 558, 684, 714
FspBI CTAG 2 cut(s) 374, 621
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 2 cut(s) 365, 540
HapII CCGG 1 cut(s) 196
Hin1II CATG 5 cut(s) 84, 230, 521, 545, 792
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 5 cut(s) 17, 35, 332, 631, 796
HpaII CCGG 1 cut(s) 196
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 3 cut(s) 242, 407, 479
Hpy188I TCNGA 4 cut(s) 40, 358, 636, 690
Hpy188III TCNNGA 5 cut(s) 57, 113, 446, 664, 763
Hpy8I GTNNAC 3 cut(s) 242, 407, 479
HpyAV CCTTC 3 cut(s) 23, 395, 460
HpyCH4III ACNGT 3 cut(s) 77, 94, 650
HpyCH4IV ACGT 2 cut(s) 244, 325
HpyCH4V TGCA 6 cut(s) 230, 242, 460, 479, 675, 781
HpyF10VI GCNNNNNNNGC 2 cut(s) 476, 485
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 2 cut(s) 244, 325
Hsp92II CATG 5 cut(s) 84, 230, 521, 545, 792
KpnI GGTACC 1 cut(s) 276
Kzo9I GATC 2 cut(s) 220, 520
LmnI GCTCC 1 cut(s) 115
LweI GCATC 2 cut(s) 329, 662
MaeI CTAG 2 cut(s) 374, 621
MaeII ACGT 2 cut(s) 244, 325
MaeIII GTNAC 1 cut(s) 247
MalI GATC 2 cut(s) 222, 522
MboI GATC 2 cut(s) 220, 520
MboII GAAGA 3 cut(s) 223, 317, 503
MhlI GDGCHC 3 cut(s) 142, 244, 481
MlsI TGGCCA 2 cut(s) 365, 540
MluCI AATT 3 cut(s) 214, 494, 783
MluNI TGGCCA 2 cut(s) 365, 540
MnlI CCTC 7 cut(s) 127, 230, 263, 295, 448, 492, 664
Mox20I TGGCCA 2 cut(s) 365, 540
MscI TGGCCA 2 cut(s) 365, 540
MseI TTAA 2 cut(s) 264, 770
MslI CAYNNNNRTG 2 cut(s) 414, 546
Msp20I TGGCCA 2 cut(s) 365, 540
MspCI CTTAAG 1 cut(s) 263
MspI CCGG 1 cut(s) 196
MwoI GCNNNNNNNGC 2 cut(s) 476, 485
NcoI CCATGG 2 cut(s) 80, 541
NdeII GATC 2 cut(s) 220, 520
NlaIII CATG 5 cut(s) 84, 230, 521, 545, 792
NlaIV GGNNCC 2 cut(s) 88, 274
PfeI GAWTC 5 cut(s) 17, 35, 332, 631, 796
PinAI ACCGGT 1 cut(s) 195
PmaCI CACGTG 1 cut(s) 245
PmlI CACGTG 1 cut(s) 245
Ppu21I YACGTR 2 cut(s) 245, 326
PspCI CACGTG 1 cut(s) 245
PspN4I GGNNCC 2 cut(s) 88, 274
PstNI CAGNNNCTG 1 cut(s) 640
RsaI GTAC 4 cut(s) 168, 274, 533, 721
RsaNI GTAC 4 cut(s) 167, 273, 532, 720
RseI CAYNNNNRTG 2 cut(s) 414, 546
SaqAI TTAA 2 cut(s) 264, 770
Sau3AI GATC 2 cut(s) 220, 520
SduI GDGCHC 3 cut(s) 142, 244, 481
SfaNI GCATC 2 cut(s) 329, 662
SgrAI CRCCGGYG 1 cut(s) 195
SmiMI CAYNNNNRTG 2 cut(s) 414, 546
SmlI CTYRAG 2 cut(s) 263, 626
SmoI CTYRAG 2 cut(s) 263, 626
SpeI ACTAGT 1 cut(s) 373
Sse9I AATT 3 cut(s) 214, 494, 783
SspI AATATT 1 cut(s) 438
SspMI CTAG 2 cut(s) 374, 621
StyI CCWWGG 2 cut(s) 80, 541
TaaI ACNGT 3 cut(s) 77, 94, 650
TaiI ACGT 2 cut(s) 247, 328
TaqI TCGA 2 cut(s) 292, 559
TasI AATT 3 cut(s) 214, 494, 783
TatI WGTACW 2 cut(s) 166, 531
TfiI GAWTC 5 cut(s) 17, 35, 332, 631, 796
Tru1I TTAA 2 cut(s) 264, 770
Tru9I TTAA 2 cut(s) 264, 770
TscAI CASTG 4 cut(s) 18, 97, 393, 653
TspRI CASTG 4 cut(s) 18, 97, 393, 653
Vha464I CTTAAG 1 cut(s) 263
VneI GTGCAC 2 cut(s) 240, 477
XapI RAATTY 2 cut(s) 214, 783
XspI CTAG 2 cut(s) 374, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.