RchiOBHm_Chr7g0177921

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000800
Physical Location & Seq
Forward (+)
735620 .. 746115
10496 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15853

Sequence Viewer

Length: 5094 bp
ATGACCTCCTCCGAAGCCGACGTTCTGATTTCCGACCTCTCCACAGGTAAAGCAGGGATTCATGGACGCATTTCGGCCTACCTACTTGTGTTCAAAGAACCCCTCAAAACCTGTGCCCAAAAGCAATCTCTTATTACCGATTACTTTCCCTTCCTCAACCGAGCGCTTACTCTTCTCCGGCGAAAGCTAGGTCATCCTTCAAAGTTGAATGAGAAATTCGCAGCCGAATGGTTCGCTGTTTACCGACTCTGTGTCGACTGCATTGCCGTCATCTCCAGGATTCTAACGAAACCACTTCTGCTCCTTGACGAAGGGTTCAAGATCCTGCGGTGTTTCGCGCGTTGGCGTCGTTGGGAGGATGGCGAGAGGGAGGGACTAGCAATTTTAAAGGGCATCAAGGAGATGCAACGCCTCCCTGTCAGTGACGTCACATCCAACTTATATTTGCCGGATTTGGATGCCGATGCCGATGACGCAGGGGAAGCAACATTGCTTGCCAAAACGGTGCCGTTGCTGTTTGTTTGCGCAGCAAAGAATAAGAAGCCCAAGCATCCTGACCAGTATCACCGCCTCTATCATATTGCTACCGAGTTTCATACATGGACCAGTGTATTGGATAAAGATCCCCGCCAGCTCGTGCTCGAGGAGCAAGCGAGCTATCTTGCGACTTGTACCATCTTTTTAGTTGGCAAGGTTGATCAATTTGACTCCAAATTTGATCTTGCAAGCAAGTTTTGTGACCTAATTATCACCACATCAGTCCAAGGATCTCCTCACCTAACGAAGCTATGTGATTCCCTTTATCAAATTGGTGTGCGAATGTTTAGTAACAAAGAATTTAAACAGGCTTCAGACACTTTGAAGTTGTATTGTCAGGCAACATGGCTTATAATCGAAGGAAAACTAAAGGAGGCAGAAACTAGATTATCAAATTATTACAGCTTGGAATTATTTGGCAAGTGGTGTGAAGCAAGTACTTTACTACTCCAAGTTCTTAGTCAATCTGAGGGTACTCATGCAGTGCAAGGAGTTATAAAAGACTGGGCTGAAAAGTGGGCTGCTGAACATTTTTCTAATAGACTGCCAGGTCCCATCTCGTTGGTGGAGCAATGGGTGGAGATTCAGGTAAAACTCGTAAAAGGTCATGAAAATGGGGATGATGTGGGCACTTTGTTAGGCAATTTGTTTCTATCTTCTAGCAAAGTATCAAGAAAGAAGATCTTGCAGCAGGAACTTCTATTTTATGTTCAAATGCAAAATGACAACCCCAACTTTTGTAAGAGAATGCAAATGATGATTAAGGATTTACTAGAGAAAGATGGGTTTGGCACACTAATTGATTGGTTAGAGGAATCAAGCTGTTTAAGACTGTGTGGAGTCAGAGGTCCAAAATACTGCACTCAATGCTTGTCACAAGCACTTGAAGTTGCTTCTGGCCATCAATTAGCAGTGGCATATTGTTTACGATCACTTTGCACCGAGGAATATGAACCAAACTCAGAGAAAGTGTTTCAAGATATTCAGTCTGCCCTTGCTGTGTGGTCGAGCATTCCTACTTCAGATGGCTCCATGGTGCCTGAAAATATAATGAAGATACTTTATTCTGTAATTGATTTGCTATCCCTCAAGGGTCGCGTAGACTTTCACTGTACTATATACAAGGTTATGATTAAGATCTATACCCAGAGAGGAGTCTCGTTGAATAATTTGTTGGCCAAATTGTGGGAATCCAGAAGGATCAGTCATGCACTCTGCCCTTCTCCAGTTCAAGAGGCATTTATGAGCCTGTCAGTTGTTTTTGGCGAAAAGTTAAAGTTAACTGAGTTTTGGATAGAGTGTTTTAGTAAATCCCAGCCATTGGTAGTAGGGTTTCATAACAATTTCTCATCTTCCCAATCCAATATAACAGTTCATAATGTTGAAGAAGTAACTTCTGCACTAATTTCAAGTGTTTCTGAATCTGGTTCTTACCTAGCTGGATATATGTACTACGATTTGTGTGAAAAATTGGTTTCAAATGGGCGTTCAGTTGAGGCTCTTCCGTATGCAATGGAAGCCCACCGCTTACGAGTAGAACTATTCAAACAGAAATTTCCATGGTCTTTTGAAGGACGCAGTGAAGGGAATCACGTGCTAGTCTGTAGATCAGTTGCTAGTGAACTATGGTCCTTTGATACTAATTCATGTGACTTGGACATCTGCTATATTAGCCCCTGGAATGCACTTCAGTGCTATCTTGAGAGCATTCTTCAGGTAGGGGTTATCCATGAAATACTGGGAAATGGAGCTGAGGCCGAATATTTTTTGGATCGGGGAAAAAGTCTTTCCTGCTCACATAGCTTGACTCTTTTTAACATTGTTTTTTCTTCCTTTTTGGGAGAACTATACCATAAGAAGCAGCGTTGGGCTTCAGCAAAAAAGGAACTTCAAAGTTCCAGGGAACTCTTGAAGGATGGCATAATAGATGGTTTTTGCTCAAAATGCAAAGTGATAGTTGAAGTAACTGTTACACATAATCTTGCTGATTTATACCAAAGCCCCATCAGTGAAATTGAGAGTGCATCTGCAGAGAAATTATCTACTGATCTCTACAAAGAAACTCTAGCCAAGCTACATACTTCTGAGTGGAAAAAGTCTATTGCTTGTCTTGAAGAACAAGATGGTACTATTTTAGTCAAAGATGGTAATTCACAACTTGAAGGCAGTATTGAGTGCAATTCTCCCAATACATTGTGTACGAAGGAACTTTCCTGGGATTTGGATTTGCAAAACTGTGTAGTATCATTTGGGGGTGATGTACCATGCATATGCAAAACAATGGGGTGTTGGCATTGTCTTACAGGGAAAGTAACAAAATCTGGCTTAGTGACTGACGCTATAAAGTTGAAGTGGGAACTAGTACACCGTCGTCTTTCACTCAAGCTGCTCACAGGATTAGGAAAATGCTTGGAGAATGATGGCCAAATTCATGAAGCATGTGAAATTATATTTCAAAGCATATCAGTATTAGTTGGACCAAACCCATTTTGTCGCGCAAGCAAATCAACTTTCTTGCCTGATACGATGGGAAAGGAAATCAGTGCTCAGCGGAAACATATAAACCTATATCATCTAATGGGAAGGGAAATCCCAGGAGATATGCTGAATGTTGAGCGTGCTGTAATACTTTACAACCTATGTTGGTTGTCTTTGAAGGACCGTAAGAAGGATACCAGAAATAATCCCCATGTGCTGATTGATATTCCTATATCAAAACTAGTGTCTTGGTTGATGCTTTCATTCGTCCTCTGCCGTGAGGTTCCTGCACTTTTTCAGAAGGTTTCGAGATTGCTGGCTGCTATTTTCTCACTGTCTACCTCAAGTGTGCTTGAAGCTCCATTGTCAACTTCTTGCAAAGATCTCTGTGGACGTCATTGGGTTTGTTTTTTTCATCAAGCTTCACTTGGAATTCAGTTTAATTACCTGTTTTCCATGAAAGAAGTCCAGCAGGGTGCAGACACTAAGGGTTCACACATGGCATCAGGAGCACAGAGTTTACCTAGGCTCGCACCAGAATCTATTCTAAACCTTAAAGACTTTGTAAAAGAATTTTTTGAAGGCCTTCCTGGCACCACAGTCATCTGTCTAAGTTTACTTGGCTGCCCATATACTAGTATGTTTCAGGAATTATTGTCTTGTTCATCTGCTCCTGCATGGATTATGGTGTCTCGCTTAAACCTTAAGAATCTTCCTATACATATTCTTCTTCCGGTGCATTCAGTACCAGGAGGAGATTCAGATGGTGTTACAATTTCTGGTGAGAATGATGGTGGTCAGTGGTGCTGTCCATGGGGTTCCACTGTGGTTGATGATGTGGCTCCGAATTTTAGAATGCTATTGGAGGAGAATTTCAATTTAGCAAAGTACGAGTTAGCTTTAAGTCCTGCTGAAAAGAAAGATCATATATATAATGTGTGGGAAGCGAGACGGAGGATTGATGATCGCCTTTGTACATTGCTGAAAAACTTGGAAGATTCATGGTTTGGTAGTACTTTGAAATATGTGCTTCTTGGTGAAGTGTCCAACTGCAAGGATCTGGACACGTTATGTGAGAACCTTGTGAGGAAATTGAAATCTCAATTCACGGACATAGATTCTAGTCTTCTGAAAGTCATCGTCCTTGGGGTGTCCAAATATGGCTTTAAAGGAGGCACAGAACAAGTTATGTTGGAGTTTTGCCTTAAGAAAGGTAGTTTCTTTAGTGATGTTCATTTCAAGGATGACCATTGTGCTGATGAATTGCGTGGCTTTGCTTCCAAACTACATGAAGCAGTGAAGAAGGTCAAAGGCTTTAATAGAATTATAAAGCCGATAATCTTAGTCCCAGACTTTGACGTCCAGATGCTTCCTTGGGAGAATTTACCGGTACTAAAGAACCAGGAGGTTTATCGCATGCCATCAGTTTGGAGTATATTGGCAGCATATAGAAATGAGCGCAAGATCAATCCTTTGGATGCCTATTATTTGTTAAACCCTGATGGTAGCCTGAATTGCAAACCCGATTTTGACAAATTGTTTAGAGATAATAAGTTGAAGGGGAAGGTTGGACATGCACCCCCAACTGAAAAACTGGCAGCAGCTTTGGAAAGCAGGGATCTCTTCATATATATAGGCCATGGAGATGGGTCGTTATATATTCCTAATGATAGGATCGAGAGACTCCAAAACTGTGCTGGGGCTTTCCTAATGGGATGCAGTAGTGCTTCTTTGAAACTAGATGGGTGTTACGTTCCACGAGGTCCTCCCCTATCGTATCTGCTTGCCGGTTCTCCTGTCATTCTCGGCAATTTATGGGAAGTAACAGGCCTTGACATTGATAAATTTACACATGCTATGCTTGTCTCTTGGTTTAAAGATAGGTCTTACCGTAAAGATTGCGATCAGAAGCTAATGATTGGAACATGCATGAGTCGAGCCCGTGATAAGTGCAGTCATTTTCCTTGCTTGGTTGGGGCATCACCGGTCTGTTATGGAGTTCCAACAGAACTCAAAGGAACTATGGAAGTAGCCACTCCTAGCACTGAGCTAAGCCCAACAACCTGCCTTGGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

1697

Amino Acids

190.51

Weight (kDa)

6.68

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 8 - 276 1.4e-55 Separase-like, TPR repeats
Separin_C PF03568 1232 - 1618 1.6e-69 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 890, 1034, 4343
AasI GACNNNNNNGTC 2 cut(s) 1086, 4373
AatII GACGTC 3 cut(s) 429, 3412, 4377
Acc16I TGCGCA 1 cut(s) 526
Acc36I ACCTGC 1 cut(s) 5087
AccB1I GGYRCC 3 cut(s) 505, 1573, 3608
AccB7I CCANNNNNTGG 2 cut(s) 1722, 1858
AccI GTMKAC 3 cut(s) 255, 1638, 3353
AccII CGCG 4 cut(s) 338, 340, 1635, 3033
AciI CCGC 5 cut(s) 328, 568, 628, 2062, 3088
AclWI GGATC 8 cut(s) 316, 617, 775, 1745, 2316, 4080, 4641, 4697
AcoI YGGCCR 3 cut(s) 1435, 1713, 2959
AcuI CTGAAG 5 cut(s) 834, 1542, 2210, 2234, 2394
AcvI CACGTG 1 cut(s) 2131
AcyI GRCGYC 4 cut(s) 346, 426, 3409, 4374
AfeI AGCGCT 1 cut(s) 165
AfiI CCNNNNNNNGG 4 cut(s) 1722, 1858, 3205, 5085
AflII CTTAAG 2 cut(s) 3719, 4220
AflIII ACRYGT 1 cut(s) 4080
AgeI ACCGGT 2 cut(s) 4402, 4999
AhdI GACNNNNNGTC 1 cut(s) 251
AhlI ACTAGT 3 cut(s) 2896, 3256, 3650
AjnI CCWGG 9 cut(s) 275, 1084, 2213, 2435, 2750, 3130, 3604, 3763, 4416
AjuI GAANNNNNNNTTGG 2 cut(s) 1694, 1726
AleI CACNNNNGTG 1 cut(s) 2227
Alw21I GWGCWC 3 cut(s) 642, 3085, 3529
Alw26I GTCTC 5 cut(s) 1699, 3711, 3958, 4690, 4885
AlwI GGATC 8 cut(s) 316, 617, 775, 1745, 2316, 4080, 4641, 4697
Ama87I CYCGRG 1 cut(s) 641
Aor51HI AGCGCT 1 cut(s) 165
AoxI GGCC 8 cut(s) 75, 1435, 1713, 2292, 2959, 3598, 4651, 4843
ArsI GACNNNNNNTTYG 6 cut(s) 175, 207, 698, 730, 3245, 3277
AsiGI ACCGGT 2 cut(s) 4402, 4999
Asp700I GAANNNNTTC 2 cut(s) 3558, 3600
AspA2I CCTAGG 1 cut(s) 3539
AspLEI GCGC 5 cut(s) 166, 340, 527, 3035, 4476
AspS9I GGNCC 7 cut(s) 603, 1088, 1385, 2166, 3014, 3196, 4778
AsuHPI GGTGA 7 cut(s) 557, 742, 767, 2804, 3809, 4064, 4989
AvaI CYCGRG 1 cut(s) 641
AvaII GGWCC 7 cut(s) 603, 1088, 1385, 2166, 3014, 3196, 4778
AvrII CCTAGG 1 cut(s) 3539
BaeGI GKGCMC 2 cut(s) 118, 1169
BalI TGGCCA 3 cut(s) 1437, 1715, 2961
BanI GGYRCC 3 cut(s) 505, 1573, 3608
BanII GRGCYC 1 cut(s) 4957
BauI CACGAG 2 cut(s) 635, 4773
BbrPI CACGTG 1 cut(s) 2131
BbsI GAAGAC 1 cut(s) 4133
Bbv12I GWGCWC 3 cut(s) 642, 3085, 3529
BbvCI CCTCAGC 1 cut(s) 2289
BceAI ACGGC 3 cut(s) 251, 493, 3276
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 1455, 1489
BciT130I CCWGG 9 cut(s) 277, 1086, 2215, 2437, 2752, 3132, 3606, 3765, 4418
BciVI GTATCC 1 cut(s) 3202
BclI TGATCA 1 cut(s) 697
BcoDI GTCTC 5 cut(s) 1699, 3711, 3958, 4690, 4885
BcuI ACTAGT 3 cut(s) 2896, 3256, 3650
BfmI CTRYAG 2 cut(s) 2140, 2565
BfoI RGCGCY 1 cut(s) 167
BfrI CTTAAG 2 cut(s) 3719, 4220
BfuAI ACCTGC 1 cut(s) 5087
BfuI GTATCC 1 cut(s) 3202
BglI GCCNNNNNGGC 1 cut(s) 3606
BglII AGATCT 3 cut(s) 1218, 1674, 3397
BlnI CCTAGG 1 cut(s) 3539
BlpI GCTNAGC 2 cut(s) 3084, 5066
BmcAI AGTACT 2 cut(s) 976, 4030
Bme1390I CCNGG 9 cut(s) 277, 1086, 2215, 2437, 2752, 3132, 3606, 3765, 4418
Bme18I GGWCC 7 cut(s) 603, 1088, 1385, 2166, 3014, 3196, 4778
BmeRI GACNNNNNGTC 1 cut(s) 251
BmeT110I CYCGRG 1 cut(s) 641
BmgT120I GGNCC 7 cut(s) 603, 1088, 1385, 2166, 3014, 3196, 4778
BmiI GGNNCC 8 cut(s) 507, 1090, 1567, 1575, 3300, 3610, 3835, 3858
BmrFI CCNGG 9 cut(s) 277, 1086, 2215, 2437, 2752, 3132, 3606, 3765, 4418
BmrI ACTGGG 2 cut(s) 1051, 2285
BmuI ACTGGG 2 cut(s) 1051, 2285
BpiI GAAGAC 1 cut(s) 4133
BpmI CTGGAG 2 cut(s) 259, 1746
Bpu10I CCTNAGC 1 cut(s) 2289
Bpu1102I GCTNAGC 2 cut(s) 3084, 5066
BpuEI CTTGAG 4 cut(s) 1610, 2258, 2903, 3343
BsaAI YACGTR 1 cut(s) 2131
BsaBI GATNNNNATC 3 cut(s) 621, 1673, 4917
BsaHI GRCGYC 4 cut(s) 346, 426, 3409, 4374
BsaWI WCCGGW 3 cut(s) 3748, 4402, 4999
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 4 cut(s) 1722, 1858, 3205, 5085
Bse118I RCCGGY 3 cut(s) 4402, 4802, 4999
Bse1I ACTGG 6 cut(s) 559, 606, 1046, 1763, 2280, 4614
Bse3DI GCAATG 5 cut(s) 261, 488, 1115, 2055, 3992
Bse8I GATNNNNATC 3 cut(s) 621, 1673, 4917
BseBI CCWGG 9 cut(s) 277, 1086, 2215, 2437, 2752, 3132, 3606, 3765, 4418
BseJI GATNNNNATC 3 cut(s) 621, 1673, 4917
BseLI CCNNNNNNNGG 4 cut(s) 1722, 1858, 3205, 5085
BseMI GCAATG 5 cut(s) 261, 488, 1115, 2055, 3992
BseMII CTCAG 7 cut(s) 996, 1512, 1812, 2280, 2613, 3098, 5052
BseNI ACTGG 6 cut(s) 559, 606, 1046, 1763, 2280, 4614
BseRI GAGGAG 5 cut(s) 659, 762, 1704, 3783, 3896
BseSI GKGCMC 2 cut(s) 118, 1169
BseYI CCCAGC 2 cut(s) 1851, 4712
BsgI GTGCAG 5 cut(s) 1381, 1920, 3288, 3513, 4987
Bsh1236I CGCG 4 cut(s) 338, 340, 1635, 3033
BshFI GGCC 8 cut(s) 77, 1437, 1715, 2294, 2961, 3600, 4653, 4845
BshNI GGYRCC 3 cut(s) 505, 1573, 3608
BshTI ACCGGT 2 cut(s) 4402, 4999
BsiHKAI GWGCWC 3 cut(s) 642, 3085, 3529
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 6 cut(s) 178, 449, 3749, 4403, 4803, 5000
BslFI GGGAC 3 cut(s) 387, 1074, 4346
BslI CCNNNNNNNGG 4 cut(s) 1722, 1858, 3205, 5085
BsmAI GTCTC 5 cut(s) 1699, 3711, 3958, 4690, 4885
BsmBI CGTCTC 1 cut(s) 3958
BsmFI GGGAC 3 cut(s) 387, 1074, 4346
BsmI GAATGC 6 cut(s) 1290, 1548, 2224, 2244, 3754, 3876
BsnI GGCC 8 cut(s) 77, 1437, 1715, 2294, 2961, 3600, 4653, 4845
BsoBI CYCGRG 1 cut(s) 641
Bsp1286I GDGCHC 6 cut(s) 118, 642, 1169, 3085, 3529, 4957
Bsp1407I TGTACA 1 cut(s) 3989
Bsp1720I GCTNAGC 2 cut(s) 3084, 5066
Bsp19I CCATGG 4 cut(s) 1569, 2096, 3827, 4654
BspACI CCGC 5 cut(s) 328, 568, 628, 2062, 3088
BspANI GGCC 8 cut(s) 77, 1437, 1715, 2294, 2961, 3600, 4653, 4845
BspCNI CTCAG 7 cut(s) 997, 1511, 1813, 2281, 2614, 3097, 5053
BspFNI CGCG 4 cut(s) 338, 340, 1635, 3033
BspHI TCATGA 2 cut(s) 1144, 2968
BspLI GGNNCC 8 cut(s) 507, 1090, 1567, 1575, 3300, 3610, 3835, 3858
BspMAI CTGCAG 1 cut(s) 2569
BspMI ACCTGC 1 cut(s) 5087
BspPI GGATC 8 cut(s) 316, 617, 775, 1745, 2316, 4080, 4641, 4697
BspQI GCTCTTC 1 cut(s) 2043
BspT107I GGYRCC 3 cut(s) 505, 1573, 3608
BspTI CTTAAG 2 cut(s) 3719, 4220
BsrDI GCAATG 5 cut(s) 261, 488, 1115, 2055, 3992
BsrFI RCCGGY 3 cut(s) 4402, 4802, 4999
BsrGI TGTACA 1 cut(s) 3989
BsrI ACTGG 6 cut(s) 559, 606, 1046, 1763, 2280, 4614
BssAI RCCGGY 3 cut(s) 4402, 4802, 4999
BssNI GRCGYC 4 cut(s) 346, 426, 3409, 4374
BssSI CACGAG 2 cut(s) 635, 4773
BssT1I CCWWGG 9 cut(s) 763, 1569, 2096, 3539, 3827, 4159, 4388, 4654, 5083
Bst2BI CACGAG 2 cut(s) 635, 4773
Bst2UI CCWGG 9 cut(s) 277, 1086, 2215, 2437, 2752, 3132, 3606, 3765, 4418
Bst6I CTCTTC 3 cut(s) 177, 2043, 4643
BstACI GRCGYC 4 cut(s) 346, 426, 3409, 4374
BstAFI CTTAAG 2 cut(s) 3719, 4220
BstAPI GCANNNNNTGC 1 cut(s) 1404
BstAUI TGTACA 1 cut(s) 3989
BstBAI YACGTR 1 cut(s) 2131
BstDSI CCRYGG 4 cut(s) 1569, 2096, 3827, 4654
BstFNI CGCG 4 cut(s) 338, 340, 1635, 3033
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 5 cut(s) 166, 340, 527, 3035, 4476
BstMAI GTCTC 5 cut(s) 1699, 3711, 3958, 4690, 4885
BstNI CCWGG 9 cut(s) 277, 1086, 2215, 2437, 2752, 3132, 3606, 3765, 4418
BstNSI RCATGY 5 cut(s) 2979, 4435, 4592, 4871, 4944
BstSCI CCNGG 9 cut(s) 275, 1084, 2213, 2435, 2750, 3130, 3604, 3763, 4416
BstSFI CTRYAG 2 cut(s) 2140, 2565
BstSLI GKGCMC 2 cut(s) 118, 1169
BstUI CGCG 4 cut(s) 338, 340, 1635, 3033
BstV2I GAAGAC 1 cut(s) 4133
BstX2I RGATCY 8 cut(s) 321, 622, 767, 1218, 1674, 3397, 4072, 4633
BstXI CCANNNNNNTGG 4 cut(s) 613, 1099, 4443, 4661
BstYI RGATCY 8 cut(s) 321, 622, 767, 1218, 1674, 3397, 4072, 4633
BsuI GTATCC 1 cut(s) 3202
BsuRI GGCC 8 cut(s) 77, 1437, 1715, 2294, 2961, 3600, 4653, 4845
BtgI CCRYGG 4 cut(s) 1569, 2096, 3827, 4654
BtsI GCAGTG 4 cut(s) 1026, 1455, 2122, 4317
BveI ACCTGC 1 cut(s) 5087
CciI TCATGA 2 cut(s) 1144, 2968
CfoI GCGC 5 cut(s) 166, 340, 527, 3035, 4476
Cfr10I RCCGGY 3 cut(s) 4402, 4802, 4999
Cfr13I GGNCC 7 cut(s) 603, 1088, 1385, 2166, 3014, 3196, 4778
CseI GACGC 5 cut(s) 75, 335, 482, 2121, 2882
CspAI ACCGGT 2 cut(s) 4402, 4999
CspCI CAANNNNNGTGG 2 cut(s) 3826, 3861
DraI TTTAAA 4 cut(s) 387, 841, 4183, 4891
DrdI GACNNNNNNGTC 2 cut(s) 1086, 4373
DriI GACNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 2 cut(s) 1086, 4373
EaeI YGGCCR 3 cut(s) 1435, 1713, 2959
Eam1104I CTCTTC 3 cut(s) 177, 2043, 4643
Eam1105I GACNNNNNGTC 1 cut(s) 251
EarI CTCTTC 3 cut(s) 177, 2043, 4643
Eco130I CCWWGG 9 cut(s) 763, 1569, 2096, 3539, 3827, 4159, 4388, 4654, 5083
Eco147I AGGCCT 2 cut(s) 3600, 4845
Eco24I GRGCYC 1 cut(s) 4957
Eco47I GGWCC 7 cut(s) 603, 1088, 1385, 2166, 3014, 3196, 4778
Eco47III AGCGCT 1 cut(s) 165
Eco57I CTGAAG 5 cut(s) 834, 1542, 2210, 2234, 2394
Eco72I CACGTG 1 cut(s) 2131
Eco88I CYCGRG 1 cut(s) 641
EcoO109I RGGNCCY 2 cut(s) 1088, 4778
EcoRI GAATTC 1 cut(s) 3447
EcoRII CCWGG 9 cut(s) 275, 1084, 2213, 2435, 2750, 3130, 3604, 3763, 4416
EcoT14I CCWWGG 9 cut(s) 763, 1569, 2096, 3539, 3827, 4159, 4388, 4654, 5083
EcoT22I ATGCAT 2 cut(s) 2807, 4946
EcoT38I GRGCYC 1 cut(s) 4957
ErhI CCWWGG 9 cut(s) 763, 1569, 2096, 3539, 3827, 4159, 4388, 4654, 5083
Esp3I CGTCTC 1 cut(s) 3958
FalI AAGNNNNNCTT 4 cut(s) 1205, 1237, 3426, 3458
FaqI GGGAC 3 cut(s) 387, 1074, 4346
FauI CCCGC 1 cut(s) 635
FauNDI CATATG 1 cut(s) 2807
FbaI TGATCA 1 cut(s) 697
FblI GTMKAC 3 cut(s) 255, 1638, 3353
FriOI GRGCYC 1 cut(s) 4957
FspI TGCGCA 1 cut(s) 526
GlaI GCGC 5 cut(s) 165, 339, 526, 3034, 4475
GsaI CCCAGC 2 cut(s) 1855, 4716
GsuI CTGGAG 2 cut(s) 259, 1746
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 8 cut(s) 77, 1437, 1715, 2294, 2961, 3600, 4653, 4845
HapII CCGG 6 cut(s) 178, 449, 3749, 4403, 4803, 5000
HgaI GACGC 5 cut(s) 75, 335, 482, 2121, 2882
HhaI GCGC 5 cut(s) 166, 340, 527, 3035, 4476
Hin1I GRCGYC 4 cut(s) 346, 426, 3409, 4374
Hin6I GCGC 5 cut(s) 164, 338, 525, 3033, 4474
HinP1I GCGC 5 cut(s) 164, 338, 525, 3033, 4474
HincII GTYRAC 3 cut(s) 256, 1818, 3384
HindII GTYRAC 3 cut(s) 256, 1818, 3384
HindIII AAGCTT 1 cut(s) 3435
HpaI GTTAAC 1 cut(s) 1818
HpaII CCGG 6 cut(s) 178, 449, 3749, 4403, 4803, 5000
HphI GGTGA 7 cut(s) 557, 742, 767, 2804, 3809, 4064, 4989
Hpy99I CGWCG 3 cut(s) 23, 351, 2910
HpyCH4IV ACGT 7 cut(s) 21, 426, 2130, 3409, 4082, 4374, 4767
HpySE526I ACGT 7 cut(s) 21, 426, 2130, 3409, 4082, 4374, 4767
Hsp92I GRCGYC 4 cut(s) 346, 426, 3409, 4374
HspAI GCGC 5 cut(s) 164, 338, 525, 3033, 4474
Ksp22I TGATCA 1 cut(s) 697
KspAI GTTAAC 1 cut(s) 1818
LguI GCTCTTC 1 cut(s) 2043
LmnI GCTCC 9 cut(s) 306, 646, 1105, 1571, 2285, 3379, 3524, 3691, 3862
MaeII ACGT 7 cut(s) 21, 426, 2130, 3409, 4082, 4374, 4767
MflI RGATCY 8 cut(s) 321, 622, 767, 1218, 1674, 3397, 4072, 4633
MhlI GDGCHC 6 cut(s) 118, 642, 1169, 3085, 3529, 4957
MlsI TGGCCA 3 cut(s) 1437, 1715, 2961
MluNI TGGCCA 3 cut(s) 1437, 1715, 2961
MlyI GAGTC 7 cut(s) 240, 701, 1386, 1701, 2338, 4692, 4957
MmeI TCCRAC 7 cut(s) 57, 459, 2992, 4086, 4188, 4564, 5042
Mox20I TGGCCA 3 cut(s) 1437, 1715, 2961
Mph1103I ATGCAT 2 cut(s) 2807, 4946
MroXI GAANNNNTTC 2 cut(s) 3558, 3600
MscI TGGCCA 3 cut(s) 1437, 1715, 2961
MslI CAYNNNNRTG 4 cut(s) 1149, 2227, 2806, 4659
Msp20I TGGCCA 3 cut(s) 1437, 1715, 2961
MspA1I CMGCKG 1 cut(s) 3088
MspCI CTTAAG 2 cut(s) 3719, 4220
MspI CCGG 6 cut(s) 178, 449, 3749, 4403, 4803, 5000
MspR9I CCNGG 9 cut(s) 277, 1086, 2215, 2437, 2752, 3132, 3606, 3765, 4418
Mva1269I GAATGC 6 cut(s) 1290, 1548, 2224, 2244, 3754, 3876
MvaI CCWGG 9 cut(s) 277, 1086, 2215, 2437, 2752, 3132, 3606, 3765, 4418
MvnI CGCG 4 cut(s) 338, 340, 1635, 3033
NcoI CCATGG 4 cut(s) 1569, 2096, 3827, 4654
NdeI CATATG 1 cut(s) 2807
NlaIV GGNNCC 8 cut(s) 507, 1090, 1567, 1575, 3300, 3610, 3835, 3858
NmeAIII GCCGAG 1 cut(s) 4800
NmuCI GTSAC 6 cut(s) 422, 427, 737, 1410, 2186, 2866
NsbI TGCGCA 1 cut(s) 526
NsiI ATGCAT 2 cut(s) 2807, 4946
NspI RCATGY 5 cut(s) 2979, 4435, 4592, 4871, 4944
OliI CACNNNNGTG 1 cut(s) 2227
PaeI GCATGC 1 cut(s) 4435
PaeR7I CTCGAG 1 cut(s) 641
PagI TCATGA 2 cut(s) 1144, 2968
PceI AGGCCT 2 cut(s) 3600, 4845
PciSI GCTCTTC 1 cut(s) 2043
PctI GAATGC 6 cut(s) 1290, 1548, 2224, 2244, 3754, 3876
PdmI GAANNNNTTC 2 cut(s) 3558, 3600
PflMI CCANNNNNTGG 2 cut(s) 1722, 1858
PfoI TCCNGGA 1 cut(s) 275
PinAI ACCGGT 2 cut(s) 4402, 4999
PleI GAGTC 7 cut(s) 240, 701, 1385, 1700, 2338, 4692, 4956
PmaCI CACGTG 1 cut(s) 2131
PmlI CACGTG 1 cut(s) 2131
PpsI GAGTC 7 cut(s) 240, 701, 1385, 1700, 2338, 4692, 4956
Ppu21I YACGTR 1 cut(s) 2131
PpuMI RGGWCCY 2 cut(s) 1088, 4778
PsiI TTATAA 3 cut(s) 890, 1034, 4343
Psp5II RGGWCCY 2 cut(s) 1088, 4778
Psp6I CCWGG 9 cut(s) 275, 1084, 2213, 2435, 2750, 3130, 3604, 3763, 4416
PspCI CACGTG 1 cut(s) 2131
PspFI CCCAGC 2 cut(s) 1851, 4712
PspGI CCWGG 9 cut(s) 275, 1084, 2213, 2435, 2750, 3130, 3604, 3763, 4416
PspN4I GGNNCC 8 cut(s) 507, 1090, 1567, 1575, 3300, 3610, 3835, 3858
PspPI GGNCC 7 cut(s) 603, 1088, 1385, 2166, 3014, 3196, 4778
PspPPI RGGWCCY 2 cut(s) 1088, 4778
PspXI VCTCGAGB 1 cut(s) 641
PstI CTGCAG 1 cut(s) 2569
PsuI RGATCY 8 cut(s) 321, 622, 767, 1218, 1674, 3397, 4072, 4633
RseI CAYNNNNRTG 4 cut(s) 1149, 2227, 2806, 4659
SalI GTCGAC 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 2043
Sau96I GGNCC 7 cut(s) 603, 1088, 1385, 2166, 3014, 3196, 4778
ScaI AGTACT 2 cut(s) 976, 4030
SchI GAGTC 7 cut(s) 240, 701, 1386, 1701, 2338, 4692, 4957
ScrFI CCNGG 9 cut(s) 277, 1086, 2215, 2437, 2752, 3132, 3606, 3765, 4418
SduI GDGCHC 6 cut(s) 118, 642, 1169, 3085, 3529, 4957
SfcI CTRYAG 2 cut(s) 2140, 2565
Sfr274I CTCGAG 1 cut(s) 641
SinI GGWCC 7 cut(s) 603, 1088, 1385, 2166, 3014, 3196, 4778
SlaI CTCGAG 1 cut(s) 641
SmiMI CAYNNNNRTG 4 cut(s) 1149, 2227, 2806, 4659
SmlI CTYRAG 7 cut(s) 641, 1625, 2237, 2918, 3358, 3719, 4220
SmoI CTYRAG 7 cut(s) 641, 1625, 2237, 2918, 3358, 3719, 4220
SpeI ACTAGT 3 cut(s) 2896, 3256, 3650
SphI GCATGC 1 cut(s) 4435
SseBI AGGCCT 2 cut(s) 3600, 4845
SsiI CCGC 5 cut(s) 328, 568, 628, 2062, 3088
SspI AATATT 1 cut(s) 2300
StuI AGGCCT 2 cut(s) 3600, 4845
StyD4I CCNGG 9 cut(s) 275, 1084, 2213, 2435, 2750, 3130, 3604, 3763, 4416
StyI CCWWGG 9 cut(s) 763, 1569, 2096, 3539, 3827, 4159, 4388, 4654, 5083
TaiI ACGT 7 cut(s) 24, 429, 2133, 3412, 4085, 4377, 4770
TaqI TCGA 7 cut(s) 255, 642, 894, 1544, 3323, 4692, 4951
TatI WGTACW 6 cut(s) 974, 1649, 1986, 2899, 3989, 4028
TseFI GTSAC 6 cut(s) 422, 427, 737, 1410, 2186, 2866
Tsp45I GTSAC 6 cut(s) 422, 427, 737, 1410, 2186, 2866
TspGWI ACGGA 3 cut(s) 2031, 3982, 4139
Van91I CCANNNNNTGG 2 cut(s) 1722, 1858
Vha464I CTTAAG 2 cut(s) 3719, 4220
VpaK11BI GGWCC 7 cut(s) 603, 1088, 1385, 2166, 3014, 3196, 4778
XceI RCATGY 5 cut(s) 2979, 4435, 4592, 4871, 4944
XcmI CCANNNNNNNNNTGG 2 cut(s) 1099, 4709
XhoI CTCGAG 1 cut(s) 641
XmaJI CCTAGG 1 cut(s) 3539
XmiI GTMKAC 3 cut(s) 255, 1638, 3353
XmnI GAANNNNTTC 2 cut(s) 3558, 3600
ZraI GACGTC 3 cut(s) 427, 3410, 4375
ZrmI AGTACT 2 cut(s) 976, 4030
Zsp2I ATGCAT 2 cut(s) 2807, 4946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.