RchiOBHm_Chr7g0222331

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000800
Physical Location & Seq
Forward (+)
43698982 .. 43715594
16613 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19898

Sequence Viewer

Length: 6444 bp
ATGGCCTCCGCCACCGAAACCTCCGAAACTCTCCTCTCCAAGCTCGAATCCGCCGATTCCGCCGCGATTCATCGCCTCATCTCCGACTACCTAAACCCATTCGCCGACCTTCACAAAAAGAAGACCTCAACCGATCACCAAACCCTACAACGCTCTCTCGCCAAAAAATTCACCCCCTTCCTCAACCGCGCCTTAGCCATCCTCCCCAAACGGCTCGCCGATCCTTCAAAGCTAGATGCCAAATTTGCCCCCGAGTTGTTCGATATTTACCGCCTCTGCCTCGACTCCATGGACGCCGTCTCCTCCCAATTTTCCGGCAAGCCCTACAAGTTTCACATCCAGAGGATCCGGTTGGTTTATGCATTCGAGGCCTGGGGCCGCCACCAGGACGCCGAGTTCGAGGCGCTTAGGGTTTTGAAGAGCTTCAAGGCGATTGAACTAGGAACGAAACCGGTGAATTTGGACCGTAGATTTGTGCCAGGTGTCGAGCAGGGAGGCTGCGATAAGGAGTTTGGGGCTTTGCTCGGAGAAGTGGTGTGGATGCTAGCCAAGTGTGCTTCGTTAATTCAAAGCAAGGAAGCCGAGGTGTATGAGCGGCTGCTTTGTATGGTTGAGGAGTTGGGACCTTGGTTTAGGGTGTTAGATGAAACTGTACATGACAAGGTGCACAGACAGCTTGCCATTTATCTCGAGAGGTGTACTCTTTTTTTAGTCCGGGAGCTATCATCCTTGGGAGCAAATCTCGTATGCAAGTTCTGCCGCATAACAGTGAGCGAGTATGCAAAGTCATCCATCAAAGATCATATGTTCAAGTGCGCCCGTCGAATATGTTCAGCTGTATTTTCATTACAAGAGACTAGTTCCTCATTTATCAGTAACACATTGTTATGCTTGTTTGACTCCCTTGTTGATGAATGCAAGGCTGAGGTCGAATATTCAGGAAAAGATTTTATTGAACTTATTGCTTATTGTGCCAATAAGTGTCGAGCTGCAAGTACAAATCTTTCTGGTACTATGAGATCTCATTCAAAAAAATTAATAACTCACTTTGATCAGGATCCACCTTTAAATTTGATTCTAAGACTTTATGCTACTGGTTTGCACTACATTGATTGTAGAATAAAATCAAAGGATGAGGATCTTACATATTCTGGAGGTGCAATTAGAACGTTGCTTGATGATGAGGATACAATGACTGATTTGTCAGATTGTCTTGATTCATTAGAAAGTTATTTCCAAATTGGTACGAATGATATTTCCAAAAGTGGGTTTTCCACGAGTCACATGCAGAAGGATTTAAAGGATTATCTTCTGCCTTATTTTAATGCATTGGAGTTCTTATGCCATCCACTTGCTGACTTGGTATATTCGGGAAAGAAACAGATTTTTGCTGATAATGGAGCTGCCTCTGTTTCTAGTGAACTGTGTGTTATCCAGGGTGCATTTCATCAGTTATATGATTTGTTCCTTTCTTTACAAACGTACAGAATTACTTGTGAGATAGATATAGATGGATTTAAGCACAACAGCATCCTTCGTGCTTCTGTGGCTGCTTTTATGCTTTCCACCAGAACGAAGCTTAACATGCAGAAGAGTGCGCAAATACTGAACCATGTCATTACCAGCGAATGGATTCAACTTGATGGTCTAAAGCATATATATGTCTCTCTCTACAATACTGGTACATGTTTGTACAAAAACAAGGAGCTGAATGAGGCTTCAGAGGCTTTGAAGCTTTCCTGCAAGGCATCTTGGACTCGTGTTATACATCTCTGTGAACTGTTTGCAGGCAAATTAAAGGTCGCCAGTGCTGATCCTTCTGAAGATACTATTTTAGATGTCTTGAATGAGTCGTGTACAGGAAGTGCTTTTCTATTGGATATTCTTAACCAAGTTGATAATCATGAGGTGAAAAGAGCTATGGCAGAGTGCCTTGAAACTTGGTCTATTGCAGCAAATTTGTTTGGGAGGCTGCCAGGTCCAATGTCTGTGGTGCAGCAGTGGGTTAAGATGGAATGTAAATGCTGTAACAATATGCATATTGAGGATAGCAGTCCAACCTTATACAGTCTGCTGTCATCTTCTAAGAAAATATCAAAGAAGACAACTGAGATAATCTTGGAGCAGGGACTTCTTGCTTACGAGAAAATGTATGCTGTGAACCCCGAGTTTTGTCAGAAGATGCAAATGCAAATTATAAACATACTACTGCAAGATGTGTATGTGACACCAGACAGTTGGTTACAAAAAGCAAGAATTTTAGTGAGAAAGGGAAGAGCGTTAAAACTTTATGGGATCAAAGGTCTAAAAGACTGTATTCATTGTTTATCAGAAGCAATATCTTCACTTAGTAAGATGTACGATAACACCAGTATCCATCAAACATCTCCTCGCCATGAATTAGCTGTGGCATATTGTTTACGCGCGCTCTGTACCCAGGAGGATGAACCACAATCAGAGAAAATGTTTCAAGATATCCGTGCTGCCCTTGATGCATGGTTGGGCATTTCTACTACAGACATGTGCTCTCCAGATGACAATTGCTCTCCAGATGACAAATGTTTAATGGTGTCTGAAAATACGATGATGCTCCTATACAATATTATTGATTTGTTGTCTATCAAGGGTCACATGGATTATTACCATGATATATATAGGCTTTTGATTAGATTATTTAACTGGAAAAATATCCCGTTAGAGAAGTGCTTGGTCAGATTGTGGGAGTGCAGGAGAGTTAGTCATGCCCTTTGTACTTCACCTGTTAATGATGCACTCATCATGAGCTTATCAGATCATTTTGGTGAACTTCCAAAGTCTATTGATTTTTGGATAGATTGTCTTAAAGGATCCCAACAATTGGTAATAGGCTTCCAACATAATTTCTCATTTCTATTTGCAAATTTACCTTGGGGCCCATGCAATCATGCAAGCTTGTTCCGATGTGAAATCACAGTTGATGAAGTTAAAAAAGATGCTCTTGAACTTATTTCAAGTGTTCCTGTATCAAGCCAGTCGGCTTATGTAGCTGCATATCTCTACTATGATTTATGTGAAAGACTAGTTTCAAATGGACAGTTAGTTGAGGCTCTATCATATGCAATGGAAGCTCACCAGTTACGCCTTGAACTATTCCAAAAGAAGTTTGCGTACTCTTTTGTACGAAAGGCTGAAAAGTACAATGAAACTAGGGATACCTATCAGAACTTTACACTCGAAGATCATAATCTGGAAGTTAAACGATCAGTTGCCCGTGAAGTTTGGTCCTTTGACACTAGTTCATGTAACTTGGAAAACTGTTATCTTAGTCCCTATAATGTACTTCAATGTTATCTTGAAAGTACTCTTCAGGTAGGGATTATCCATGAAATAGTTGGAAATGGAGCTGAGGCCGAGGTAGTTTTACAGTGGGGAAAGACTATTTCCTGCTCACAAAGCTTGCCACTATTTGCAGTTGCATTTTGTTCGGTCTTAGGAAAACTGTATCATAAGAAGCAGCTTTGGGATTTGGCAGAGAAGGAACTTCAAAGTGCCAACCAATATTTGAAGGCTAGTGCAAAAGATATTTCTTGCGTAAAGTGCAGATTGATGCAGAAAGCAACTGTTAAACTGAATCTTGGTGATTTATACCAAAGCAAATTTTATAGTTCAAGGAGTGCATCTTTAGAGACATTGTCTTATGCTGAAAGTTTGTACAAATCAGCACTTACCAAGCTAAACCTTTCTGAGTGGCAAAATTCTGTTAGTTGCCCTGGACAAACTGTACTTAAAGAAGTTGGATATTCTGCTGGCAGTATTTCTAGTCATTTTGCGGAAACCAAGGTGGAACCAAAAAGCAAGATAGAAGCTAAAAAGTCTAGGACTAAGAATGCCCGAAAACCGGTGGGGAGGGATCAAGGTTCAGTTCCTGATTATAATTTGAGGTCAACTCGGTCTAGATGCCAATCTTCTCAGAACCAAAGTGTAAGAGGTATTGGTGTCCAAGTTGGTCATCCAAAACATTTGAAACGAAGCACTGAGTGTGATTCTTCTGATAATTTAAGTAACAAGGACTTTCTTTTGGATTTGAAAACTTGTGAGGTTTCATATGGGTCTGACCTAACATGTATTTGCAACAAAATGAGGTGTTGGCATTGTCTTCCCATGGAAGTTATGGAATCTGGGCTACTGAAAAACTTTGTAGATTTGAAATGGGAGTATGTCCGCAGGCGTCTCTCTCTCAGGCTTCTGACAGGATTAGGAAATTGTTTGGAGAATCAAGGTCAAATTCATGAAACAAATGAACTTATCCTGCAAAGCGTATCGATTTTAGTCAGCAGGAACCCATTTTGTATAACTACCTCATCGATTCCGACGACTTCCTTGCTTGATTTAATGGCAAAGGAAATCCCTGGGGATGTGCTTAGTGTTGAGCGTGCGGAAGTACTGTACAGCATATGTTGGCTGTCTTTAAAGATTCGTTCCAAGAATACCAGGGTTATTTTCTCTGATCTGCCTCATATTCATCTACCAAAACTAGTGTCTTGGCTGATGTTAGCTTTTGTGCTCTGCCGTGAAGTTCCTGTACTTTTTCAGAAGGTTTCTAGATTGCTTGCTGCTATATTTTTACTTTCTGCCTCAAGTGAGCTTTTTTCTTTGTCATCTTCTTGCAAAAAGCTCCACGAAAATCATTGGGCTTCTTATTTTCATCAAGCTTCAATTGGCACCCATCTCAATTACCAATTTTTCACGAAAGTATCGGAGAGGTGTAAACTCCAACACCTTTTAAACGGTGAGGGGGCACATGTTGCTGGCTCATGCTTGGTATCAGAAACACAGAATTTACATAGGGTCGCCCCAGAGTCAATTCAAGACCTTGAAGAATTTGTGACAGAATTCTTTTCAGGCCTTCCATGCACCACAATCATCTGTATAAGTGTGCTTGGAGGCCCTTATGCTAGTTTGTTACAAGAATTGTTGTGTTTTCCTTCACGCATGCATGCATGGATTCTGGTTTCACGCTTGAACTCTAAGAATCAACCTATCAGTATGCTTCTGCCAGTGGATTCAGTATTAGAAGGAGATTCAGATGATGTTAGTGATACTGGAATAAAGGATTGGCACTGTCCATGGGGTTCCACTGTGGTTGATGATGTAGCTCCAGAGTTTAGATTGATATTGGAGGGGACTCATTCATCATCTGTGAAACATCCTGTACAAGATACAAATGAGAAAAAGCTTAACTGGTGGGTGCAGAGGAAGAATTTTGATCGCCGCCTTGGTAAATTTCTGAAGAACTTGGAAGATTCATGGTTTGGTACTTGGAAACTTGTCCTACTGGGTGAATGGTCAAACTGCAAGCAGTTGGACTTGGTACATAAGAAGCTGGTGCGCAATCTGAAATCCAAATGCAAAATGGAGATAGATGAGAGTCTTTTGAAAGTCATCCTTGGGGGTTCCAAATATGCCTTAGAAGGAGGTGCACGTGTTACACAGCTTTGTCTTAAGAAAGGTTCCTGCGTTAGTAAAGTTGGATATTTAGAGGAAGAGAAGTGTATGGCATCATGTAATGATTCTAATGGTGGCGAGAACTTGTCTGAGTTGGCCTCCAAACTAGTTTTAGAAGCAGTGAATGAGCTTGAAGGTTTACATTCTTCTTTAAATATAGAGCCAACAATATTAGTTGTGGACTACGAGGTGCAGATGCTTCCTTGGGAGAATTTACCAATCCTAAGAAACCAGGAGGTTTATCGCATGCCCTCTGTTGGGAGCATTTCGGCAACAGCCAATAGAAACTACCACAATCAAGATCAAGTTCAGAGTATTGTGACTTCATTTCCTTTGATAGATCCCTTGGATGCCTTTTATCTTTTGAATCCCAGTGGTGATCTAAATTATACACAAAATGAATTTGAGACATGGTTCAGAGATCAAAACTTGGAGGGGAAGGTCGGATGTGCACCTCCTGCTGAAGAATTGGCAGTAGCGTTGAAAAGCCATGATCTATTTATGTATTTTGGCCATGGATGTGGGCAACAATATATTCCTAGACGTGAGATTCAGAGACTGGAACACTGTGCCGCAACTCTCCTTATGGGATGCAGTAGTGGTTCTCTGAAACTGAATGGATGTTATGTTCCACAAGGTACTCCACTATCCTATCTGCTGGCCGGTTCTCCTGTCATTGTCGCCAATTTGTGGGATGTGACGGACAGGGACATTGACCGATTTGCAAAGTCCATGCTTGATTCTTGGTTGAAAGCAAGGTCGAGTCCCTGTGTGAGTTGTGCCCAATGCAACAAGAAAGTCACAAAAAAGAAATTGGCCACAAACCTGTCTTGTGAGCATAGGCCGAAGGTTGGATCATTCATGAGCGAAGCACGTAAAACTTGCCAGTTACCTTTCTTGATTGGAGCAGCACCAGTATGCTATGGTGTCCCAACTGGCATATGGAAAAAGGAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

2147

Amino Acids

242.11

Weight (kDa)

6.77

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 9 - 279 1.5e-104 Separase-like, TPR repeats
TPR_ESP1_2nd PF25113 289 - 462 1.2e-39 Separase-like, TPR repeats
Separin_C PF03568 1659 - 2066 1.4e-98 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2198, 3912
AasI GACNNNNNNGTC 1 cut(s) 1201
Acc16I TGCGCA 2 cut(s) 1599, 5369
AccB1I GGYRCC 1 cut(s) 4700
AccB7I CCANNNNNTGG 3 cut(s) 1629, 2857, 6174
AccBSI CCGCTC 1 cut(s) 595
AccII CGCG 4 cut(s) 65, 189, 2424, 2426
AclI AACGTT 1 cut(s) 1169
AcoI YGGCCR 3 cut(s) 5995, 6144, 6300
AcuI CTGAAG 5 cut(s) 1704, 1842, 3329, 5288, 5967
AcvI CACGTG 1 cut(s) 5462
AcyI GRCGYC 3 cut(s) 294, 390, 4207
AdeI CACNNNGTG 1 cut(s) 4017
AfiI CCNNNNNNNGG 9 cut(s) 385, 1266, 1629, 2857, 3877, 5741, 5742, 6174, 6335
AflII CTTAAG 1 cut(s) 5480
AflIII ACRYGT 5 cut(s) 1685, 2520, 4100, 4780, 5461
AgeI ACCGGT 2 cut(s) 451, 3877
AhlI ACTAGT 5 cut(s) 857, 3058, 3272, 4513, 5590
AjiI CACGTC 1 cut(s) 6029
AjuI GAANNNNNNNTTGG 2 cut(s) 5077, 5109
AloI GAACNNNNNNTCC 4 cut(s) 380, 412, 6096, 6128
Alw21I GWGCWC 5 cut(s) 669, 2528, 4545, 5461, 5938
Alw26I GTCTC 7 cut(s) 304, 848, 1669, 3659, 4214, 5885, 6034
Alw44I GTGCAC 3 cut(s) 665, 5457, 5934
AlwNI CAGNNNCTG 2 cut(s) 3905, 6043
Ama87I CYCGRG 3 cut(s) 251, 689, 2165
ApaI GGGCCC 1 cut(s) 2915
ApaLI GTGCAC 3 cut(s) 665, 5457, 5934
ArsI GACNNNNNNTTYG 8 cut(s) 380, 412, 455, 487, 1785, 1817, 4502, 4534
AseI ATTAAT 1 cut(s) 1037
AsiGI ACCGGT 2 cut(s) 451, 3877
Asp700I GAANNNNTTC 3 cut(s) 422, 829, 1632
AspLEI GCGC 7 cut(s) 191, 406, 818, 1600, 2426, 2428, 5370
AspS9I GGNCC 8 cut(s) 376, 463, 623, 1979, 2911, 2912, 3261, 4925
AsuC2I CCSGG 1 cut(s) 716
AsuNHI GCTAGC 1 cut(s) 544
AvaI CYCGRG 3 cut(s) 251, 689, 2165
AvaII GGWCC 4 cut(s) 463, 623, 1979, 3261
BaeGI GKGCMC 6 cut(s) 669, 2915, 4780, 5461, 5938, 6268
BaeI ACNNNNGTAYC 4 cut(s) 2356, 2389, 4716, 4749
BalI TGGCCA 2 cut(s) 5997, 6302
BamHI GGATCC 3 cut(s) 345, 1057, 2846
BanI GGYRCC 1 cut(s) 4700
BanII GRGCYC 1 cut(s) 2915
BarI GAAGNNNNNNTAC 6 cut(s) 3131, 3163, 4545, 4577, 5791, 5823
BauI CACGAG 2 cut(s) 1276, 1758
BbrPI CACGTG 1 cut(s) 5462
BbsI GAAGAC 3 cut(s) 128, 2108, 4127
Bbv12I GWGCWC 5 cut(s) 669, 2528, 4545, 5461, 5938
BbvCI CCTCAGC 2 cut(s) 924, 3384
BccI CCATC 8 cut(s) 206, 800, 1353, 1505, 1637, 2005, 2385, 4713
BceAI ACGGC 3 cut(s) 227, 281, 4533
BcgI CGANNNNNNTGC 6 cut(s) 1267, 1301, 3444, 3478, 4342, 4376
BciVI GTATCC 3 cut(s) 1180, 2384, 3184
BclI TGATCA 1 cut(s) 1051
BcnI CCSGG 1 cut(s) 716
BcoDI GTCTC 7 cut(s) 304, 848, 1669, 3659, 4214, 5885, 6034
BcuI ACTAGT 5 cut(s) 857, 3058, 3272, 4513, 5590
BfmI CTRYAG 1 cut(s) 2514
BfoI RGCGCY 1 cut(s) 407
BfrI CTTAAG 1 cut(s) 5480
BfuI GTATCC 3 cut(s) 1180, 2384, 3184
BglII AGATCT 1 cut(s) 1019
BmcAI AGTACT 2 cut(s) 3340, 4422
Bme18I GGWCC 4 cut(s) 463, 623, 1979, 3261
BmeT110I CYCGRG 3 cut(s) 251, 689, 2165
BmgBI CACGTC 1 cut(s) 6029
BmgT120I GGNCC 8 cut(s) 376, 463, 623, 1979, 2911, 2912, 3261, 4925
BmrI ACTGGG 2 cut(s) 5324, 5850
BmtI GCTAGC 1 cut(s) 548
BmuI ACTGGG 2 cut(s) 5324, 5850
BpiI GAAGAC 3 cut(s) 128, 2108, 4127
BplI GAGNNNNNCTC 8 cut(s) 685, 717, 726, 758, 3910, 3942, 5567, 5599
BpmI CTGGAG 4 cut(s) 1173, 2514, 2532, 5121
Bpu10I CCTNAGC 4 cut(s) 193, 407, 924, 3384
BpuEI CTTGAG 1 cut(s) 4600
BpuMI CCSGG 1 cut(s) 716
Bsa29I ATCGAT 2 cut(s) 4301, 4343
BsaAI YACGTR 2 cut(s) 5462, 6359
BsaBI GATNNNNATC 5 cut(s) 1056, 1137, 3195, 3222, 3940
BsaHI GRCGYC 3 cut(s) 294, 390, 4207
BsaWI WCCGGW 3 cut(s) 348, 451, 3877
BsaXI ACNNNNNCTCC 6 cut(s) 284, 314, 519, 549, 2722, 2752
Bsc4I CCNNNNNNNGG 9 cut(s) 385, 1266, 1629, 2857, 3877, 5741, 5742, 6174, 6335
Bse118I RCCGGY 3 cut(s) 451, 3877, 6146
Bse3DI GCAATG 1 cut(s) 3105
Bse8I GATNNNNATC 5 cut(s) 1056, 1137, 3195, 3222, 3940
BseCI ATCGAT 2 cut(s) 4301, 4343
BseJI GATNNNNATC 5 cut(s) 1056, 1137, 3195, 3222, 3940
BseLI CCNNNNNNNGG 9 cut(s) 385, 1266, 1629, 2857, 3877, 5741, 5742, 6174, 6335
BseMI GCAATG 1 cut(s) 3105
BseMII CTCAG 8 cut(s) 915, 2100, 3375, 3714, 3962, 4005, 4231, 5565
BsePI GCGCGC 1 cut(s) 2424
BseRI GAGGAG 4 cut(s) 23, 292, 629, 2379
BseSI GKGCMC 6 cut(s) 669, 2915, 4780, 5461, 5938, 6268
BsgI GTGCAG 5 cut(s) 2015, 2746, 3598, 5249, 5696
Bsh1236I CGCG 4 cut(s) 65, 189, 2424, 2426
BshNI GGYRCC 1 cut(s) 4700
BshTI ACCGGT 2 cut(s) 451, 3877
BshVI ATCGAT 2 cut(s) 4301, 4343
BsiHKAI GWGCWC 5 cut(s) 669, 2528, 4545, 5461, 5938
BsiHKCI CYCGRG 3 cut(s) 251, 689, 2165
BsiSI CCGG 6 cut(s) 315, 349, 452, 715, 3878, 6147
BslFI GGGAC 7 cut(s) 636, 2142, 3291, 5176, 6206, 6234, 6398
BslI CCNNNNNNNGG 9 cut(s) 385, 1266, 1629, 2857, 3877, 5741, 5742, 6174, 6335
BsmAI GTCTC 7 cut(s) 304, 848, 1669, 3659, 4214, 5885, 6034
BsmBI CGTCTC 2 cut(s) 304, 4214
BsmFI GGGAC 7 cut(s) 636, 2142, 3291, 5176, 6206, 6234, 6398
BsmI GAATGC 3 cut(s) 362, 920, 3871
BsoBI CYCGRG 3 cut(s) 251, 689, 2165
Bsp120I GGGCCC 1 cut(s) 2911
Bsp1286I GDGCHC 8 cut(s) 669, 2528, 2915, 4545, 4780, 5461, 5938, 6268
Bsp1407I TGTACA 6 cut(s) 652, 1692, 1856, 3690, 4425, 5191
Bsp19I CCATGG 4 cut(s) 288, 4140, 5105, 5998
BspCNI CTCAG 8 cut(s) 916, 2101, 3376, 3715, 3961, 4006, 4230, 5566
BspDI ATCGAT 2 cut(s) 4301, 4343
BspFNI CGCG 4 cut(s) 65, 189, 2424, 2426
BspHI TCATGA 4 cut(s) 1903, 2778, 4267, 6345
BspOI GCTAGC 1 cut(s) 548
BspQI GCTCTTC 2 cut(s) 413, 2269
BspT107I GGYRCC 1 cut(s) 4700
BspTI CTTAAG 1 cut(s) 5480
BsrBI CCGCTC 1 cut(s) 595
BsrDI GCAATG 1 cut(s) 3105
BsrFI RCCGGY 3 cut(s) 451, 3877, 6146
BsrGI TGTACA 6 cut(s) 652, 1692, 1856, 3690, 4425, 5191
BssAI RCCGGY 3 cut(s) 451, 3877, 6146
BssHII GCGCGC 1 cut(s) 2424
BssNI GRCGYC 3 cut(s) 294, 390, 4207
BssSI CACGAG 2 cut(s) 1276, 1758
Bst2BI CACGAG 2 cut(s) 1276, 1758
Bst6I CTCTTC 5 cut(s) 413, 1586, 2269, 3348, 5517
BstACI GRCGYC 3 cut(s) 294, 390, 4207
BstAFI CTTAAG 1 cut(s) 5480
BstAPI GCANNNNNTGC 3 cut(s) 756, 4784, 5942
BstAUI TGTACA 6 cut(s) 652, 1692, 1856, 3690, 4425, 5191
BstBAI YACGTR 2 cut(s) 5462, 6359
BstDSI CCRYGG 4 cut(s) 288, 4140, 5105, 5998
BstFNI CGCG 4 cut(s) 65, 189, 2424, 2426
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 7 cut(s) 191, 406, 818, 1600, 2426, 2428, 5370
BstMAI GTCTC 7 cut(s) 304, 848, 1669, 3659, 4214, 5885, 6034
BstNSI RCATGY 9 cut(s) 1288, 1588, 1689, 2524, 4104, 4784, 4975, 4979, 5734
BstSFI CTRYAG 1 cut(s) 2514
BstSLI GKGCMC 6 cut(s) 669, 2915, 4780, 5461, 5938, 6268
BstUI CGCG 4 cut(s) 65, 189, 2424, 2426
BstV2I GAAGAC 3 cut(s) 128, 2108, 4127
BstX2I RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2846, 5824
BstXI CCANNNNNNTGG 2 cut(s) 2238, 6005
BstYI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2846, 5824
Bsu15I ATCGAT 2 cut(s) 4301, 4343
BsuI GTATCC 3 cut(s) 1180, 2384, 3184
BsuTUI ATCGAT 2 cut(s) 4301, 4343
BtgI CCRYGG 4 cut(s) 288, 4140, 5105, 5998
BtgZI GCGATG 1 cut(s) 56
BtrI CACGTC 1 cut(s) 6029
BtsI GCAGTG 2 cut(s) 2006, 5610
CaiI CAGNNNCTG 2 cut(s) 3905, 6043
CciI TCATGA 4 cut(s) 1903, 2778, 4267, 6345
CfoI GCGC 7 cut(s) 191, 406, 818, 1600, 2426, 2428, 5370
Cfr10I RCCGGY 3 cut(s) 451, 3877, 6146
Cfr13I GGNCC 8 cut(s) 376, 463, 623, 1979, 2911, 2912, 3261, 4925
ClaI ATCGAT 2 cut(s) 4301, 4343
CseI GACGC 3 cut(s) 302, 398, 4196
CspAI ACCGGT 2 cut(s) 451, 3877
CspCI CAANNNNNGTGG 4 cut(s) 1971, 2006, 5104, 5139
DraI TTTAAA 5 cut(s) 1068, 1299, 4449, 4764, 5637
DraIII CACNNNGTG 1 cut(s) 4017
DrdI GACNNNNNNGTC 1 cut(s) 1201
DseDI GACNNNNNNGTC 1 cut(s) 1201
EaeI YGGCCR 3 cut(s) 5995, 6144, 6300
Eam1104I CTCTTC 5 cut(s) 413, 1586, 2269, 3348, 5517
EarI CTCTTC 5 cut(s) 413, 1586, 2269, 3348, 5517
EciI GGCGGA 2 cut(s) 40, 49
Eco147I AGGCCT 2 cut(s) 371, 4884
Eco24I GRGCYC 1 cut(s) 2915
Eco32I GATATC 1 cut(s) 2476
Eco47I GGWCC 4 cut(s) 463, 623, 1979, 3261
Eco57I CTGAAG 5 cut(s) 1704, 1842, 3329, 5288, 5967
Eco72I CACGTG 1 cut(s) 5462
Eco88I CYCGRG 3 cut(s) 251, 689, 2165
EcoO109I RGGNCCY 3 cut(s) 623, 2911, 4925
EcoRI GAATTC 1 cut(s) 4871
EcoRV GATATC 1 cut(s) 2476
EcoT22I ATGCAT 6 cut(s) 364, 1330, 2040, 2497, 4977, 4981
EcoT38I GRGCYC 1 cut(s) 2915
Esp3I CGTCTC 2 cut(s) 304, 4214
FalI AAGNNNNNCTT 4 cut(s) 2961, 2993, 5409, 5441
FaqI GGGAC 7 cut(s) 636, 2142, 3291, 5176, 6206, 6234, 6398
FauNDI CATATG 5 cut(s) 804, 3094, 4084, 4433, 6425
FbaI TGATCA 1 cut(s) 1051
FriOI GRGCYC 1 cut(s) 2915
FspI TGCGCA 2 cut(s) 1599, 5369
GlaI GCGC 7 cut(s) 190, 405, 817, 1599, 2425, 2427, 5369
GsuI CTGGAG 4 cut(s) 1173, 2514, 2532, 5121
HaeII RGCGCY 1 cut(s) 407
HapII CCGG 6 cut(s) 315, 349, 452, 715, 3878, 6147
HgaI GACGC 3 cut(s) 302, 398, 4196
HhaI GCGC 7 cut(s) 191, 406, 818, 1600, 2426, 2428, 5370
Hin1I GRCGYC 3 cut(s) 294, 390, 4207
Hin6I GCGC 7 cut(s) 189, 404, 816, 1598, 2424, 2426, 5368
HinP1I GCGC 7 cut(s) 189, 404, 816, 1598, 2424, 2426, 5368
HincII GTYRAC 1 cut(s) 3924
HindII GTYRAC 1 cut(s) 3924
HindIII AAGCTT 6 cut(s) 1577, 1733, 2929, 3433, 4689, 5213
HpaII CCGG 6 cut(s) 315, 349, 452, 715, 3878, 6147
Hpy99I CGWCG 2 cut(s) 825, 4354
HpyCH4IV ACGT 5 cut(s) 1169, 1481, 5461, 6028, 6358
HpySE526I ACGT 5 cut(s) 1169, 1481, 5461, 6028, 6358
Hsp92I GRCGYC 3 cut(s) 294, 390, 4207
HspAI GCGC 7 cut(s) 189, 404, 816, 1598, 2424, 2426, 5368
Ksp22I TGATCA 1 cut(s) 1051
LguI GCTCTTC 2 cut(s) 413, 2269
MaeII ACGT 5 cut(s) 1169, 1481, 5461, 6028, 6358
MbiI CCGCTC 1 cut(s) 595
MfeI CAATTG 3 cut(s) 2539, 2855, 4695
MflI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2846, 5824
MhlI GDGCHC 8 cut(s) 669, 2528, 2915, 4545, 4780, 5461, 5938, 6268
MlsI TGGCCA 2 cut(s) 5997, 6302
MluNI TGGCCA 2 cut(s) 5997, 6302
MlyI GAGTC 9 cut(s) 278, 893, 1288, 1750, 1859, 4847, 5158, 5416, 6256
Mox20I TGGCCA 2 cut(s) 5997, 6302
Mph1103I ATGCAT 6 cut(s) 364, 1330, 2040, 2497, 4977, 4981
MroXI GAANNNNTTC 3 cut(s) 422, 829, 1632
MscI TGGCCA 2 cut(s) 5997, 6302
MslI CAYNNNNRTG 7 cut(s) 767, 886, 1659, 1773, 2799, 6003, 6400
Msp20I TGGCCA 2 cut(s) 5997, 6302
MspA1I CMGCKG 1 cut(s) 836
MspCI CTTAAG 1 cut(s) 5480
MspI CCGG 6 cut(s) 315, 349, 452, 715, 3878, 6147
MunI CAATTG 3 cut(s) 2539, 2855, 4695
Mva1269I GAATGC 3 cut(s) 362, 920, 3871
MvnI CGCG 4 cut(s) 65, 189, 2424, 2426
NciI CCSGG 1 cut(s) 716
NcoI CCATGG 4 cut(s) 288, 4140, 5105, 5998
NdeI CATATG 5 cut(s) 804, 3094, 4084, 4433, 6425
NheI GCTAGC 1 cut(s) 544
NmeAIII GCCGAG 3 cut(s) 418, 607, 3415
NmuCI GTSAC 7 cut(s) 1280, 2224, 2627, 4864, 5803, 6181, 6283
NsbI TGCGCA 2 cut(s) 1599, 5369
NsiI ATGCAT 6 cut(s) 364, 1330, 2040, 2497, 4977, 4981
NspI RCATGY 9 cut(s) 1288, 1588, 1689, 2524, 4104, 4784, 4975, 4979, 5734
PaeI GCATGC 3 cut(s) 4975, 4979, 5734
PaeR7I CTCGAG 1 cut(s) 689
PagI TCATGA 4 cut(s) 1903, 2778, 4267, 6345
PasI CCCWGGG 1 cut(s) 4388
PauI GCGCGC 1 cut(s) 2424
PceI AGGCCT 2 cut(s) 371, 4884
PciI ACATGT 4 cut(s) 1685, 2520, 4100, 4780
PciSI GCTCTTC 2 cut(s) 413, 2269
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 396, 4349
PctI GAATGC 3 cut(s) 362, 920, 3871
PdmI GAANNNNTTC 3 cut(s) 422, 829, 1632
PflFI GACNNNGTC 2 cut(s) 296, 3670
PflMI CCANNNNNTGG 3 cut(s) 1629, 2857, 6174
PfoI TCCNGGA 1 cut(s) 714
PinAI ACCGGT 2 cut(s) 451, 3877
PleI GAGTC 9 cut(s) 278, 893, 1287, 1750, 1858, 4846, 5158, 5415, 6255
PmaCI CACGTG 1 cut(s) 5462
PmlI CACGTG 1 cut(s) 5462
PpsI GAGTC 9 cut(s) 278, 893, 1287, 1750, 1858, 4846, 5158, 5415, 6255
Ppu21I YACGTR 2 cut(s) 5462, 6359
PpuMI RGGWCCY 1 cut(s) 623
PscI ACATGT 4 cut(s) 1685, 2520, 4100, 4780
PshBI ATTAAT 1 cut(s) 1037
PsiI TTATAA 2 cut(s) 2198, 3912
Psp1406I AACGTT 1 cut(s) 1169
Psp5II RGGWCCY 1 cut(s) 623
PspCI CACGTG 1 cut(s) 5462
PspOMI GGGCCC 1 cut(s) 2911
PspPI GGNCC 8 cut(s) 376, 463, 623, 1979, 2911, 2912, 3261, 4925
PspPPI RGGWCCY 1 cut(s) 623
PstNI CAGNNNCTG 2 cut(s) 3905, 6043
PsuI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2846, 5824
PsyI GACNNNGTC 2 cut(s) 296, 3670
PteI GCGCGC 1 cut(s) 2424
PvuII CAGCTG 1 cut(s) 836
RseI CAYNNNNRTG 7 cut(s) 767, 886, 1659, 1773, 2799, 6003, 6400
SapI GCTCTTC 2 cut(s) 413, 2269
Sau96I GGNCC 8 cut(s) 376, 463, 623, 1979, 2911, 2912, 3261, 4925
ScaI AGTACT 2 cut(s) 3340, 4422
SchI GAGTC 9 cut(s) 278, 893, 1288, 1750, 1859, 4847, 5158, 5416, 6256
SduI GDGCHC 8 cut(s) 669, 2528, 2915, 4545, 4780, 5461, 5938, 6268
SfcI CTRYAG 1 cut(s) 2514
Sfr274I CTCGAG 1 cut(s) 689
SinI GGWCC 4 cut(s) 463, 623, 1979, 3261
SlaI CTCGAG 1 cut(s) 689
SmiMI CAYNNNNRTG 7 cut(s) 767, 886, 1659, 1773, 2799, 6003, 6400
SmlI CTYRAG 3 cut(s) 689, 4615, 5480
SmoI CTYRAG 3 cut(s) 689, 4615, 5480
SpeI ACTAGT 5 cut(s) 857, 3058, 3272, 4513, 5590
SphI GCATGC 3 cut(s) 4975, 4979, 5734
SseBI AGGCCT 2 cut(s) 371, 4884
SspI AATATT 4 cut(s) 935, 2602, 3539, 5655
StuI AGGCCT 2 cut(s) 371, 4884
TaiI ACGT 5 cut(s) 1172, 1484, 5464, 6031, 6361
TaqII GACCGA 3 cut(s) 3454, 3918, 6216
TauI GCSGC 6 cut(s) 65, 381, 598, 762, 5253, 6059
TseFI GTSAC 7 cut(s) 1280, 2224, 2627, 4864, 5803, 6181, 6283
Tsp45I GTSAC 7 cut(s) 1280, 2224, 2627, 4864, 5803, 6181, 6283
TspGWI ACGGA 2 cut(s) 2468, 6200
Tth111I GACNNNGTC 2 cut(s) 296, 3670
Van91I CCANNNNNTGG 3 cut(s) 1629, 2857, 6174
Vha464I CTTAAG 1 cut(s) 5480
VneI GTGCAC 3 cut(s) 665, 5457, 5934
VpaK11BI GGWCC 4 cut(s) 463, 623, 1979, 3261
VspI ATTAAT 1 cut(s) 1037
XbaI TCTAGA 2 cut(s) 3932, 4580
XceI RCATGY 9 cut(s) 1288, 1588, 1689, 2524, 4104, 4784, 4975, 4979, 5734
XcmI CCANNNNNNNNNTGG 4 cut(s) 3368, 4147, 5389, 6423
XhoI CTCGAG 1 cut(s) 689
XmnI GAANNNNTTC 3 cut(s) 422, 829, 1632
ZrmI AGTACT 2 cut(s) 3340, 4422
Zsp2I ATGCAT 6 cut(s) 364, 1330, 2040, 2497, 4977, 4981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.