Rh7CG010500

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000800
Physical Location & Seq
Forward (+)
800575 .. 808810
8236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG010500.1

Sequence Viewer

Length: 4059 bp
ATGACCTCCTCCGAAGCCGACGTTCTGATTTCCGACCTCTCCACAGGTAAAGCAGGGATTCATGGACGCATTTCGGCCTACCTACTTGTGTTCAAAGAACCCCTCAAAACCTGTGCCCAAAAGCAATCTCTTATTACCGATTACTTTCCCTTCCTCAACCGAGCGCTTACTCTTCTCCGGCGAAAGCTAGGTCATCCTTCAAAGTTGAATGAGAAATTCGCAGCCGAATGGTTCGCTGTTTACCGACTCTGTGTCGACTGCATTGCCGTCATCTCCAGGATTCTAACGAAACCACTTCTGCTCCTTGAAGAAGGGTTCAAGATCCTGCGGTGTTTCGCGCGTTGGCGTCGTTGGGAGGATGGCGAGAGGGAGGGACTAGCAATTTTAAAGGGCATCAAGGAGATGCAACGCCTCCCTGTCAGTGACGTCACATCCAACTTATATTTGCCGGATTTGGATGCCGATGCCGATGATGCAGGGGAAGCAACATTGCTTGCCAAAACGGTGCCGTTGCTGTTTGTTTGCGCAGCAAAGAATAAGAAGCCCAAGCATCCTGACCAGTATCACCGCCTCTATCATATTGCCACCGAGTTTCATACATGGACCAGTGTATTGGATAAAGATCCCCGCCAGCTCGTGCTCGAGGAGCAAGCGAGCTATCTTGCGACTTGTACCATCTTTTTAGTTGGCAAGGTTGATCAATTTGACTCCAAATTTGATCTTGCAAGCAAGTTTTGTGACCTAATTATCACCACATCAGTCCAAGGATCTCCTCACCTAACGAAGCTATGTGATTCCCTTTATCAAATTGGTGTGCGAATGTTTAGTAACAAAGAATTTAAACAGGCTTCAGACACTTTGAAGTTGTATTGTCAGGCAACATGGCTTATAATCGAAGGAAAACTAAAGGAGGCAGAAACTAGATTATCAAATTATTACAGCTTGGAATTATTTGGCAAGTGGTGTGAAGCAAGTACTTTACTACTCCAAGTTCTTAGTCAATCTGAGGGTACTCATGCAGTGCAAGGAGTTATAAAAGACTGGGCTGAAAAGTGGGCTGCTGAACATTTTTCTAATAGACTGCCAGGTCCCATCTCGTTGGTGGAGCAATGGGTGGAGGTAAAACTCGTAAAAGGTCATGAAAATGGGGATGATGTGGGCACTTTGTTAGGCAATTTGTTTCTATCTTCTAGCAAAGTATCAAGAAAGAAGATCTTGCAGCAGGAACTTCTATTTTATGTTCAAATGCAAAATGACAACCCCAACTTTTGTAAGAGAATGCAAATGATGATTAAGGATTTACTAGAGAAAGATGGGTTTGGCACACTAATTGATTGGTTAGAGGAATCAAGCTGTTTAAGACTGTGTGGAGTCAGAGGTCCAAAATACTGCACTCAATGCTTGTCACAAGCACTTGAAGTTGCTTCTGGCCATCAATTAGCTGTGGCATATTGTTTTCGATCACTTTGCACCGAGGAATATGAACCGAACTCAGAGAAAGTGTTTCAAGATATTCAGTCTGCCCTTGCTGTGTGGTCGAGCATTCCTACTTCAGATGGCTCCATGGTGCCTGAAAATATAATGAAGATACTTTATTCTGTAATTGATTTGCTATCCCTCAAGGGTCGCGTAGACTTTCACTGTACTATATACAAGGTTATGATTAAGATCTATACCCAGAGAGGAGTCTCGTTGAATAATTTGTTGGCCAAATTGTGGGAATCCAGAAGGATCAGTCATGCACTCTGCCCTTCTCCAGTTCAAGAGGCATTTATGAGCCTGTCAGATGTTTTTGGCGAAAAGTTAAAGTTAACTGAGTTTTGGATAGAGTGTTTTAGTAAATCCCAGCCATTGGTAGTAGGGTTTCATAACAATTTCTCATCTTCTCAATCCAATATAACAGTTCATAATGTTGAAGAAGTAACTTCTGCACTAATTTCAAGTGTTTCTGAATCTGGTTCTTACCTAGCTGGATATATGTACTACGATTTGTGTGAAAAATTGGTTTCAAATGGGCGTTCAATTGAGGCTCTTCCGTATGCAATGGAAGCCCACCGCTTACGAGTAGAACTATTCAAACAGAAATTTCCATGGTCTTTTGAAGGACGCGGTGAAGGGAATCACGTGCTAGTCTGTAGATCAGTTGCTAGTGAACTATGGTCCTTTGATACTAATTCATGTGACTTGGACATCTGCTATATTAGCCCCTGGAATGCACTTCAGTGCTATCTTGAGAGCATTCTTCAGGTAGGGGTTATCCATGAAATACTGGGAAATGGAGCTGAGGCCGAATATTTTTTGGATCGGGGAAAAAGTCTTTCCTGCTCACATAGCTTGACTCTTTTTAACATTGTTTTTTCTTCCTTTTTGGGAGAACTATACCATAAGAAGCAGCGTTGGGCTTCAGCAAAAAAGGAACTTCAAAGTTCCAGGGAACTCTTGAAGGATGGCATAATAGATGGTTTTTGCTCAAAATGCAAAGTGATACTTGAAGTAACTGTTACACATAATCTTGCTGAATTATACCAAAGCCCCATCAGTGAAATTGAGAGTGCATCTGCAGAGAAATTATCTACTGATCTCTACAAAGAAACTCTAGCCAAGCTACATACTTCTGAGTGGAAAAAGTCTATTGCTTGTCTTGAAGAACAAGATGGTACTATTTTAGTCAAAGATGGTAATTCACAACTTGAAGGCAGTATTGAGTGCAATTCTCCCAATACATTGTGTACGAAGGAACTTCCCTGGGATTTGGATTTGCAAAACTGTGTAGTATCATTTGGGGGTGATGTACCATGCATATGCAAAACAATGGGGTGTTGGCATTGTCTTACAGGGAAAGTAACAAAATCTGGCTTAGTGACTGACGCTATAAAGTTGAAGTGGGAACTAGTACACCGTCGTCTTTCACGCAAGCTGCTCACAGGATTAGGAAAATGCTTGGAGAATGATGGCCAAATTCATGAAGCATGTGAAATTATATTTCAAAGCATATCAGTAATAGTTGGACCAAACCCATTTTGTCGCGCAAGCAAATCAACTTTCTTTCCTGATACGATGGGAAAGGAAATCAGTGCTCAGCGGAAACATATAAACCTATATCATCTAATGGGAAGGGAAATCCCAGGAGATATGCTGAATGTTGAGCGTGCTGTAATACTTTACAACCTATGTTGGTTGTCTTTGAAGGACCGTAAGAAGGATACCAGAAATAATCCCCATGTGCTGATTGATATTCCTATATCAAAACTAGTGTCTTGGTTGATGCTTTCATTCGTCCTCTGCCGTGAGGTTCCTGCACTTTTTCAGAAGGTTTCGAGATTGCTGGCTGCTATTTTCTCACTGTCTACCTCAAGTGTGCTTGAAGCTCCATTGTCAACTTCTTGCAAAGATCTCTGTGGACGTCATTGGGTTTGTTTTTTTCATCAAGCTTCACTTGGAATTCAGTTTAATTACCTGTTTTCCATGAAAGAAGTCCAGCAGGGTGCAGACACTAAGGGTTCACACATGGCATCAGGAGCACAGAGTTTACCTAGGCTCGCACCAGAATCTATTCTAAACCTTAAAGACTTTGTAAAAGAATTTTTTGAAGGCCTTCCTGGCACCACAGTCATCTGTCTAAGTTTACTTGGCTGCCCATATACTAGTATGTTTCAGGAATTATTGTCTTGTTCATCTGCTCCTGCATGGATTATGGTGTCTCGCTTAAACCCTAAGAATCTTCCTATACATATTCTTCTTCCGGTGCATTCAGTACCAGGAGGAGATTCAGATGGTGTTACAATTTCTGGTGAGAATGATGGTGGTCAGTGGCGCTGTCCATGGGGTTCCACTGTGGTTGATGATGTAGCTCCGAATTTTAGAATGCTATTGGAGGAGAATTTCAATTTAGCAAATTACGAGTTAGCTTTAAGTCCTGCTGAAAAGAAAGATCATATATATAATGTGTGGGAAGCGAGACGGAGGATTGATGATCGCCTTTGTACATTGCTGAAGTGTGTTATACTCTCCTCCTCTCTATGTAATTTTGGTCTTCTACTATATTTTATTTTTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

1352

Amino Acids

152.16

Weight (kDa)

6.41

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 8 - 276 1e-55 Separase-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 890, 1034
AasI GACNNNNNNGTC 1 cut(s) 1086
AatII GACGTC 2 cut(s) 429, 3406
Acc16I TGCGCA 1 cut(s) 526
AccB1I GGYRCC 3 cut(s) 505, 1567, 3602
AccB7I CCANNNNNTGG 2 cut(s) 1716, 1852
AccI GTMKAC 3 cut(s) 255, 1632, 3347
AccII CGCG 5 cut(s) 338, 340, 1629, 2109, 3027
AciI CCGC 6 cut(s) 328, 568, 628, 2056, 2109, 3082
AclWI GGATC 5 cut(s) 316, 617, 775, 1739, 2310
AcoI YGGCCR 3 cut(s) 1429, 1707, 2953
AcsI RAATTY 9 cut(s) 215, 713, 836, 2084, 2958, 3441, 3581, 3856, 3880
AcuI CTGAAG 6 cut(s) 834, 1536, 2204, 2228, 2388, 4013
AcvI CACGTG 1 cut(s) 2125
AcyI GRCGYC 3 cut(s) 346, 426, 3403
AfeI AGCGCT 1 cut(s) 165
AfiI CCNNNNNNNGG 3 cut(s) 1716, 1852, 3199
AhdI GACNNNNNGTC 1 cut(s) 251
AhlI ACTAGT 3 cut(s) 2890, 3250, 3644
AjnI CCWGG 8 cut(s) 275, 1084, 2207, 2429, 2744, 3124, 3598, 3757
AjuI GAANNNNNNNTTGG 2 cut(s) 1688, 1720
AleI CACNNNNGTG 1 cut(s) 2221
Alw21I GWGCWC 3 cut(s) 642, 3079, 3523
Alw26I GTCTC 3 cut(s) 1693, 3705, 3952
AlwI GGATC 5 cut(s) 316, 617, 775, 1739, 2310
Ama87I CYCGRG 1 cut(s) 641
Aor51HI AGCGCT 1 cut(s) 165
AoxI GGCC 6 cut(s) 75, 1429, 1707, 2286, 2953, 3592
ApeKI GCWGC 8 cut(s) 221, 527, 1058, 1219, 2392, 2917, 3329, 3633
ApoI RAATTY 9 cut(s) 215, 713, 836, 2084, 2958, 3441, 3581, 3856, 3880
ArsI GACNNNNNNTTYG 6 cut(s) 175, 207, 698, 730, 3239, 3271
Asp700I GAANNNNTTC 2 cut(s) 3552, 3594
AspA2I CCTAGG 1 cut(s) 3533
AspLEI GCGC 5 cut(s) 166, 340, 527, 3029, 3816
AspS9I GGNCC 6 cut(s) 603, 1088, 1379, 2160, 3008, 3190
AsuHPI GGTGA 6 cut(s) 557, 742, 767, 2123, 2798, 3803
AvaI CYCGRG 1 cut(s) 641
AvaII GGWCC 6 cut(s) 603, 1088, 1379, 2160, 3008, 3190
AvrII CCTAGG 1 cut(s) 3533
BaeGI GKGCMC 2 cut(s) 118, 1163
BalI TGGCCA 3 cut(s) 1431, 1709, 2955
BanI GGYRCC 3 cut(s) 505, 1567, 3602
BauI CACGAG 1 cut(s) 635
BbrPI CACGTG 1 cut(s) 2125
BbsI GAAGAC 1 cut(s) 4025
Bbv12I GWGCWC 3 cut(s) 642, 3079, 3523
BbvCI CCTCAGC 1 cut(s) 2283
BbvI GCAGC 8 cut(s) 233, 539, 1045, 1231, 2404, 2904, 3316, 3620
BceAI ACGGC 3 cut(s) 251, 493, 3270
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 1449, 1483
BciT130I CCWGG 8 cut(s) 277, 1086, 2209, 2431, 2746, 3126, 3600, 3759
BciVI GTATCC 1 cut(s) 3196
BclI TGATCA 1 cut(s) 697
BcoDI GTCTC 3 cut(s) 1693, 3705, 3952
BcuI ACTAGT 3 cut(s) 2890, 3250, 3644
BfmI CTRYAG 2 cut(s) 2134, 2559
BfoI RGCGCY 2 cut(s) 167, 3817
BfuI GTATCC 1 cut(s) 3196
BglI GCCNNNNNGGC 1 cut(s) 3600
BglII AGATCT 3 cut(s) 1212, 1668, 3391
BisI GCNGC 8 cut(s) 222, 528, 1059, 1220, 2393, 2918, 3330, 3634
BlnI CCTAGG 1 cut(s) 3533
BlpI GCTNAGC 1 cut(s) 3078
BlsI GCNGC 8 cut(s) 223, 529, 1060, 1221, 2394, 2919, 3331, 3635
BmcAI AGTACT 1 cut(s) 976
Bme1390I CCNGG 8 cut(s) 277, 1086, 2209, 2431, 2746, 3126, 3600, 3759
Bme18I GGWCC 6 cut(s) 603, 1088, 1379, 2160, 3008, 3190
BmeRI GACNNNNNGTC 1 cut(s) 251
BmeT110I CYCGRG 1 cut(s) 641
BmgT120I GGNCC 6 cut(s) 603, 1088, 1379, 2160, 3008, 3190
BmiI GGNNCC 7 cut(s) 507, 1090, 1561, 1569, 3294, 3604, 3829
BmrFI CCNGG 8 cut(s) 277, 1086, 2209, 2431, 2746, 3126, 3600, 3759
BmrI ACTGGG 2 cut(s) 1051, 2279
BmsI GCATC 9 cut(s) 393, 402, 448, 454, 463, 559, 2564, 3255, 3521
BmuI ACTGGG 2 cut(s) 1051, 2279
BpiI GAAGAC 1 cut(s) 4025
BpmI CTGGAG 2 cut(s) 259, 1740
Bpu10I CCTNAGC 1 cut(s) 2283
Bpu1102I GCTNAGC 1 cut(s) 3078
BpuEI CTTGAG 3 cut(s) 1604, 2252, 3337
BsaAI YACGTR 1 cut(s) 2125
BsaBI GATNNNNATC 2 cut(s) 621, 1667
BsaHI GRCGYC 3 cut(s) 346, 426, 3403
BsaWI WCCGGW 1 cut(s) 3742
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 3 cut(s) 1716, 1852, 3199
Bse1I ACTGG 5 cut(s) 559, 606, 1046, 1757, 2274
Bse3DI GCAATG 5 cut(s) 261, 488, 1115, 2049, 3986
Bse8I GATNNNNATC 2 cut(s) 621, 1667
BseBI CCWGG 8 cut(s) 277, 1086, 2209, 2431, 2746, 3126, 3600, 3759
BseGI GGATG 7 cut(s) 193, 364, 431, 463, 550, 1156, 2452
BseJI GATNNNNATC 2 cut(s) 621, 1667
BseLI CCNNNNNNNGG 3 cut(s) 1716, 1852, 3199
BseMI GCAATG 5 cut(s) 261, 488, 1115, 2049, 3986
BseMII CTCAG 6 cut(s) 996, 1506, 1806, 2274, 2607, 3092
BseNI ACTGG 5 cut(s) 559, 606, 1046, 1757, 2274
BseRI GAGGAG 7 cut(s) 659, 762, 1698, 3777, 3890, 4000, 4003
BseSI GKGCMC 2 cut(s) 118, 1163
BseXI GCAGC 8 cut(s) 233, 539, 1045, 1231, 2404, 2904, 3316, 3620
BseYI CCCAGC 1 cut(s) 1845
BsgI GTGCAG 4 cut(s) 1375, 1914, 3282, 3507
Bsh1236I CGCG 5 cut(s) 338, 340, 1629, 2109, 3027
BshFI GGCC 6 cut(s) 77, 1431, 1709, 2288, 2955, 3594
BshNI GGYRCC 3 cut(s) 505, 1567, 3602
BsiHKAI GWGCWC 3 cut(s) 642, 3079, 3523
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 3 cut(s) 178, 449, 3743
BslFI GGGAC 2 cut(s) 387, 1074
BslI CCNNNNNNNGG 3 cut(s) 1716, 1852, 3199
BsmAI GTCTC 3 cut(s) 1693, 3705, 3952
BsmBI CGTCTC 1 cut(s) 3952
BsmFI GGGAC 2 cut(s) 387, 1074
BsmI GAATGC 6 cut(s) 1284, 1542, 2218, 2238, 3748, 3870
BsnI GGCC 6 cut(s) 77, 1431, 1709, 2288, 2955, 3594
BsoBI CYCGRG 1 cut(s) 641
Bsp1286I GDGCHC 5 cut(s) 118, 642, 1163, 3079, 3523
Bsp1407I TGTACA 1 cut(s) 3983
Bsp1720I GCTNAGC 1 cut(s) 3078
Bsp19I CCATGG 3 cut(s) 1563, 2090, 3821
BspACI CCGC 6 cut(s) 328, 568, 628, 2056, 2109, 3082
BspANI GGCC 6 cut(s) 77, 1431, 1709, 2288, 2955, 3594
BspCNI CTCAG 6 cut(s) 997, 1505, 1807, 2275, 2608, 3091
BspFNI CGCG 5 cut(s) 338, 340, 1629, 2109, 3027
BspHI TCATGA 2 cut(s) 1138, 2962
BspLI GGNNCC 7 cut(s) 507, 1090, 1561, 1569, 3294, 3604, 3829
BspMAI CTGCAG 1 cut(s) 2563
BspPI GGATC 5 cut(s) 316, 617, 775, 1739, 2310
BspQI GCTCTTC 1 cut(s) 2037
BspT107I GGYRCC 3 cut(s) 505, 1567, 3602
BsrDI GCAATG 5 cut(s) 261, 488, 1115, 2049, 3986
BsrGI TGTACA 1 cut(s) 3983
BsrI ACTGG 5 cut(s) 559, 606, 1046, 1757, 2274
BssNI GRCGYC 3 cut(s) 346, 426, 3403
BssSI CACGAG 1 cut(s) 635
BssT1I CCWWGG 5 cut(s) 763, 1563, 2090, 3533, 3821
Bst2BI CACGAG 1 cut(s) 635
Bst2UI CCWGG 8 cut(s) 277, 1086, 2209, 2431, 2746, 3126, 3600, 3759
Bst6I CTCTTC 2 cut(s) 177, 2037
BstACI GRCGYC 3 cut(s) 346, 426, 3403
BstAPI GCANNNNNTGC 1 cut(s) 1398
BstAUI TGTACA 1 cut(s) 3983
BstBAI YACGTR 1 cut(s) 2125
BstDSI CCRYGG 3 cut(s) 1563, 2090, 3821
BstF5I GGATG 7 cut(s) 193, 364, 431, 463, 550, 1156, 2452
BstFNI CGCG 5 cut(s) 338, 340, 1629, 2109, 3027
BstH2I RGCGCY 2 cut(s) 167, 3817
BstHHI GCGC 5 cut(s) 166, 340, 527, 3029, 3816
BstMAI GTCTC 3 cut(s) 1693, 3705, 3952
BstNI CCWGG 8 cut(s) 277, 1086, 2209, 2431, 2746, 3126, 3600, 3759
BstNSI RCATGY 1 cut(s) 2973
BstSCI CCNGG 8 cut(s) 275, 1084, 2207, 2429, 2744, 3124, 3598, 3757
BstSFI CTRYAG 2 cut(s) 2134, 2559
BstSLI GKGCMC 2 cut(s) 118, 1163
BstUI CGCG 5 cut(s) 338, 340, 1629, 2109, 3027
BstV1I GCAGC 8 cut(s) 233, 539, 1045, 1231, 2404, 2904, 3316, 3620
BstV2I GAAGAC 1 cut(s) 4025
BstX2I RGATCY 6 cut(s) 321, 622, 767, 1212, 1668, 3391
BstXI CCANNNNNNTGG 2 cut(s) 613, 1099
BstYI RGATCY 6 cut(s) 321, 622, 767, 1212, 1668, 3391
BsuI GTATCC 1 cut(s) 3196
BsuRI GGCC 6 cut(s) 77, 1431, 1709, 2288, 2955, 3594
BtgI CCRYGG 3 cut(s) 1563, 2090, 3821
BtsCI GGATG 7 cut(s) 193, 364, 431, 463, 550, 1156, 2452
BtsI GCAGTG 1 cut(s) 1026
CciI TCATGA 2 cut(s) 1138, 2962
CfoI GCGC 5 cut(s) 166, 340, 527, 3029, 3816
Cfr13I GGNCC 6 cut(s) 603, 1088, 1379, 2160, 3008, 3190
CseI GACGC 4 cut(s) 75, 335, 2115, 2876
CspCI CAANNNNNGTGG 2 cut(s) 3820, 3855
DraI TTTAAA 2 cut(s) 387, 841
DrdI GACNNNNNNGTC 1 cut(s) 1086
DriI GACNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 1086
EaeI YGGCCR 3 cut(s) 1429, 1707, 2953
Eam1104I CTCTTC 2 cut(s) 177, 2037
Eam1105I GACNNNNNGTC 1 cut(s) 251
EarI CTCTTC 2 cut(s) 177, 2037
Eco130I CCWWGG 5 cut(s) 763, 1563, 2090, 3533, 3821
Eco147I AGGCCT 1 cut(s) 3594
Eco47I GGWCC 6 cut(s) 603, 1088, 1379, 2160, 3008, 3190
Eco47III AGCGCT 1 cut(s) 165
Eco57I CTGAAG 6 cut(s) 834, 1536, 2204, 2228, 2388, 4013
Eco72I CACGTG 1 cut(s) 2125
Eco88I CYCGRG 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 1088
EcoRI GAATTC 1 cut(s) 3441
EcoRII CCWGG 8 cut(s) 275, 1084, 2207, 2429, 2744, 3124, 3598, 3757
EcoT14I CCWWGG 5 cut(s) 763, 1563, 2090, 3533, 3821
EcoT22I ATGCAT 1 cut(s) 2801
ErhI CCWWGG 5 cut(s) 763, 1563, 2090, 3533, 3821
Esp3I CGTCTC 1 cut(s) 3952
FalI AAGNNNNNCTT 6 cut(s) 1199, 1231, 2472, 2504, 3420, 3452
FaqI GGGAC 2 cut(s) 387, 1074
FauI CCCGC 1 cut(s) 635
FauNDI CATATG 1 cut(s) 2801
FbaI TGATCA 1 cut(s) 697
FblI GTMKAC 3 cut(s) 255, 1632, 3347
Fnu4HI GCNGC 8 cut(s) 222, 528, 1059, 1220, 2393, 2918, 3330, 3634
FokI GGATG 7 cut(s) 180, 371, 418, 470, 537, 1163, 2459
Fsp4HI GCNGC 8 cut(s) 222, 528, 1059, 1220, 2393, 2918, 3330, 3634
FspI TGCGCA 1 cut(s) 526
GlaI GCGC 5 cut(s) 165, 339, 526, 3028, 3815
GluI GCNGC 8 cut(s) 222, 528, 1059, 1220, 2393, 2918, 3330, 3634
GsaI CCCAGC 1 cut(s) 1849
GsuI CTGGAG 2 cut(s) 259, 1740
HaeII RGCGCY 2 cut(s) 167, 3817
HaeIII GGCC 6 cut(s) 77, 1431, 1709, 2288, 2955, 3594
HapII CCGG 3 cut(s) 178, 449, 3743
HgaI GACGC 4 cut(s) 75, 335, 2115, 2876
HhaI GCGC 5 cut(s) 166, 340, 527, 3029, 3816
Hin1I GRCGYC 3 cut(s) 346, 426, 3403
Hin6I GCGC 5 cut(s) 164, 338, 525, 3027, 3814
HinP1I GCGC 5 cut(s) 164, 338, 525, 3027, 3814
HincII GTYRAC 3 cut(s) 256, 1812, 3378
HindII GTYRAC 3 cut(s) 256, 1812, 3378
HindIII AAGCTT 1 cut(s) 3429
HpaI GTTAAC 1 cut(s) 1812
HpaII CCGG 3 cut(s) 178, 449, 3743
HphI GGTGA 6 cut(s) 557, 742, 767, 2123, 2798, 3803
Hpy99I CGWCG 3 cut(s) 23, 351, 2904
HpyCH4IV ACGT 4 cut(s) 21, 426, 2124, 3403
HpySE526I ACGT 4 cut(s) 21, 426, 2124, 3403
Hsp92I GRCGYC 3 cut(s) 346, 426, 3403
HspAI GCGC 5 cut(s) 164, 338, 525, 3027, 3814
Ksp22I TGATCA 1 cut(s) 697
KspAI GTTAAC 1 cut(s) 1812
LguI GCTCTTC 1 cut(s) 2037
LmnI GCTCC 9 cut(s) 306, 646, 1105, 1565, 2279, 3373, 3518, 3685, 3856
Lsp1109I GCAGC 8 cut(s) 233, 539, 1045, 1231, 2404, 2904, 3316, 3620
LweI GCATC 9 cut(s) 393, 402, 448, 454, 463, 559, 2564, 3255, 3521
MaeII ACGT 4 cut(s) 21, 426, 2124, 3403
MfeI CAATTG 1 cut(s) 2022
MflI RGATCY 6 cut(s) 321, 622, 767, 1212, 1668, 3391
MhlI GDGCHC 5 cut(s) 118, 642, 1163, 3079, 3523
MlsI TGGCCA 3 cut(s) 1431, 1709, 2955
MluNI TGGCCA 3 cut(s) 1431, 1709, 2955
MlyI GAGTC 5 cut(s) 240, 701, 1380, 1695, 2332
MmeI TCCRAC 3 cut(s) 57, 459, 2986
Mox20I TGGCCA 3 cut(s) 1431, 1709, 2955
Mph1103I ATGCAT 1 cut(s) 2801
MroXI GAANNNNTTC 2 cut(s) 3552, 3594
MscI TGGCCA 3 cut(s) 1431, 1709, 2955
MslI CAYNNNNRTG 3 cut(s) 1143, 2221, 2800
Msp20I TGGCCA 3 cut(s) 1431, 1709, 2955
MspA1I CMGCKG 1 cut(s) 3082
MspI CCGG 3 cut(s) 178, 449, 3743
MspR9I CCNGG 8 cut(s) 277, 1086, 2209, 2431, 2746, 3126, 3600, 3759
MunI CAATTG 1 cut(s) 2022
Mva1269I GAATGC 6 cut(s) 1284, 1542, 2218, 2238, 3748, 3870
MvaI CCWGG 8 cut(s) 277, 1086, 2209, 2431, 2746, 3126, 3600, 3759
MvnI CGCG 5 cut(s) 338, 340, 1629, 2109, 3027
NcoI CCATGG 3 cut(s) 1563, 2090, 3821
NdeI CATATG 1 cut(s) 2801
NlaIV GGNNCC 7 cut(s) 507, 1090, 1561, 1569, 3294, 3604, 3829
NmuCI GTSAC 6 cut(s) 422, 427, 737, 1404, 2180, 2860
NsbI TGCGCA 1 cut(s) 526
NsiI ATGCAT 1 cut(s) 2801
NspI RCATGY 1 cut(s) 2973
OliI CACNNNNGTG 1 cut(s) 2221
PaeR7I CTCGAG 1 cut(s) 641
PagI TCATGA 2 cut(s) 1138, 2962
PasI CCCWGGG 1 cut(s) 2745
PceI AGGCCT 1 cut(s) 3594
PciSI GCTCTTC 1 cut(s) 2037
PctI GAATGC 6 cut(s) 1284, 1542, 2218, 2238, 3748, 3870
PdmI GAANNNNTTC 2 cut(s) 3552, 3594
PflMI CCANNNNNTGG 2 cut(s) 1716, 1852
PfoI TCCNGGA 1 cut(s) 275
PkrI GCNGC 8 cut(s) 223, 529, 1060, 1221, 2394, 2919, 3331, 3635
PleI GAGTC 5 cut(s) 240, 701, 1379, 1694, 2332
PmaCI CACGTG 1 cut(s) 2125
PmlI CACGTG 1 cut(s) 2125
PpsI GAGTC 5 cut(s) 240, 701, 1379, 1694, 2332
Ppu21I YACGTR 1 cut(s) 2125
PpuMI RGGWCCY 1 cut(s) 1088
PsiI TTATAA 2 cut(s) 890, 1034
Psp5II RGGWCCY 1 cut(s) 1088
Psp6I CCWGG 8 cut(s) 275, 1084, 2207, 2429, 2744, 3124, 3598, 3757
PspCI CACGTG 1 cut(s) 2125
PspFI CCCAGC 1 cut(s) 1845
PspGI CCWGG 8 cut(s) 275, 1084, 2207, 2429, 2744, 3124, 3598, 3757
PspN4I GGNNCC 7 cut(s) 507, 1090, 1561, 1569, 3294, 3604, 3829
PspPI GGNCC 6 cut(s) 603, 1088, 1379, 2160, 3008, 3190
PspPPI RGGWCCY 1 cut(s) 1088
PspXI VCTCGAGB 1 cut(s) 641
PstI CTGCAG 1 cut(s) 2563
PsuI RGATCY 6 cut(s) 321, 622, 767, 1212, 1668, 3391
RseI CAYNNNNRTG 3 cut(s) 1143, 2221, 2800
SalI GTCGAC 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 2037
SatI GCNGC 8 cut(s) 222, 528, 1059, 1220, 2393, 2918, 3330, 3634
Sau96I GGNCC 6 cut(s) 603, 1088, 1379, 2160, 3008, 3190
ScaI AGTACT 1 cut(s) 976
SchI GAGTC 5 cut(s) 240, 701, 1380, 1695, 2332
ScrFI CCNGG 8 cut(s) 277, 1086, 2209, 2431, 2746, 3126, 3600, 3759
SduI GDGCHC 5 cut(s) 118, 642, 1163, 3079, 3523
SfaNI GCATC 9 cut(s) 393, 402, 448, 454, 463, 559, 2564, 3255, 3521
SfcI CTRYAG 2 cut(s) 2134, 2559
Sfr274I CTCGAG 1 cut(s) 641
SinI GGWCC 6 cut(s) 603, 1088, 1379, 2160, 3008, 3190
SlaI CTCGAG 1 cut(s) 641
SmiMI CAYNNNNRTG 3 cut(s) 1143, 2221, 2800
SmlI CTYRAG 4 cut(s) 641, 1619, 2231, 3352
SmoI CTYRAG 4 cut(s) 641, 1619, 2231, 3352
SpeI ACTAGT 3 cut(s) 2890, 3250, 3644
SseBI AGGCCT 1 cut(s) 3594
SsiI CCGC 6 cut(s) 328, 568, 628, 2056, 2109, 3082
SspI AATATT 1 cut(s) 2294
StuI AGGCCT 1 cut(s) 3594
StyD4I CCNGG 8 cut(s) 275, 1084, 2207, 2429, 2744, 3124, 3598, 3757
StyI CCWWGG 5 cut(s) 763, 1563, 2090, 3533, 3821
TaiI ACGT 4 cut(s) 24, 429, 2127, 3406
TaqI TCGA 6 cut(s) 255, 642, 894, 1459, 1538, 3317
TatI WGTACW 5 cut(s) 974, 1643, 1980, 2893, 3983
TseFI GTSAC 6 cut(s) 422, 427, 737, 1404, 2180, 2860
TseI GCWGC 8 cut(s) 221, 527, 1058, 1219, 2392, 2917, 3329, 3633
Tsp45I GTSAC 6 cut(s) 422, 427, 737, 1404, 2180, 2860
TspGWI ACGGA 2 cut(s) 2025, 3976
Van91I CCANNNNNTGG 2 cut(s) 1716, 1852
VpaK11BI GGWCC 6 cut(s) 603, 1088, 1379, 2160, 3008, 3190
XapI RAATTY 9 cut(s) 215, 713, 836, 2084, 2958, 3441, 3581, 3856, 3880
XceI RCATGY 1 cut(s) 2973
XcmI CCANNNNNNNNNTGG 1 cut(s) 1099
XhoI CTCGAG 1 cut(s) 641
XmaJI CCTAGG 1 cut(s) 3533
XmiI GTMKAC 3 cut(s) 255, 1632, 3347
XmnI GAANNNNTTC 2 cut(s) 3552, 3594
ZraI GACGTC 2 cut(s) 427, 3404
ZrmI AGTACT 1 cut(s) 976
Zsp2I ATGCAT 1 cut(s) 2801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.