AT5G28550

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000800
Physical Location & Seq
Reverse (-)
10557722 .. 10559779
2058 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G28550.1

Sequence Viewer

Length: 861 bp
ATGGAGCTTGCTATTCATGATGGAGAATATGGTGCAACATTTGTGATTTCCGACAAGGAAATGAGGAATCTTACTAACAAACCAGCAACTACACTCATTGTAGAGCAAATGATAGGATATACTGATAGCTCGACTTCTTGGCAGGGTATACCACAAGCTGATGTGATTCTAAGACTTTATTCAGCTGGACGATGGACCCCTGACAAGGAATGCCTTGGAACTGACTTTGACAAGACTCTGCTAAACGTGGTTATGGCTGCTTTTATTTTTTCCATGAGAACCCTACGTAATGTAGATGTCAAACGCTTACTTGCAATTGTCACAAATCAGTGGAAGATCACTAACCGTCTAGTTGAGGATGTAATTGCTAGTCCGTGGATTTCACCTCCAGAGCTCAAATTTTTATTTGCTTCATTTCACGATATCAGTGTAGCTTTCTACAATAATAAGCACCTAGAAAAGGCTTCCATGGTGTTCAAGCTATGTATTAGAACAGTGTGGACTTGTGTTAGACTCCTCTGTCAGATATATGTAAATAAGTCTGACCTATCTGAGGATTGTCTTCCCAAAGAAGCAATTATTGATTTTGTGAGTGAAGCATGTAGCAAATCTGCATTTTATCTAGATAATCTCCATCAACATGGTGCACGAGAAACCGAGAAGTTGCATGTGTTTATTCTAGAAAATTGGCCTGAAGCTGAAGACCTCATCAAAAAGTTGCCAGACCCTACACCGATAGTAAAGCAATGGGTTAAGCTAGTACATGAGCTTTTCTTGGGATGTGCACAAAGGTGTAGCCGTGTAGGTATGATCTCCCTCAACCATGTTTTTGATGTCCCTCATATAAGTGGCATACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

286

Amino Acids

32.63

Weight (kDa)

6.14

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 519
AccI GTMKAC 1 cut(s) 148
AcsI RAATTY 1 cut(s) 398
AcuI CTGAAG 2 cut(s) 714, 720
AfaI GTAC 1 cut(s) 762
AfiI CCNNNNNNNGG 3 cut(s) 205, 460, 553
AgsI TTSAA 1 cut(s) 478
AleI CACNNNNGTG 1 cut(s) 790
Alw21I GWGCWC 3 cut(s) 396, 649, 787
Alw44I GTGCAC 2 cut(s) 645, 783
AoxI GGCC 1 cut(s) 689
ApaLI GTGCAC 2 cut(s) 645, 783
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 1 cut(s) 398
AspS9I GGNCC 1 cut(s) 195
AsuHPI GGTGA 1 cut(s) 375
AvaII GGWCC 1 cut(s) 195
BaeGI GKGCMC 2 cut(s) 649, 787
BanII GRGCYC 1 cut(s) 396
BauI CACGAG 1 cut(s) 648
BbsI GAAGAC 2 cut(s) 554, 708
Bbv12I GWGCWC 3 cut(s) 396, 649, 787
BbvI GCAGC 1 cut(s) 244
BccI CCATC 3 cut(s) 14, 186, 642
BceAI ACGGC 1 cut(s) 783
BfaI CTAG 6 cut(s) 350, 369, 455, 623, 680, 758
BisI GCNGC 1 cut(s) 258
BlsI GCNGC 1 cut(s) 259
Bme18I GGWCC 1 cut(s) 195
BmgT120I GGNCC 1 cut(s) 195
BmiI GGNNCC 1 cut(s) 197
BpiI GAAGAC 2 cut(s) 554, 708
BpmI CTGGAG 1 cut(s) 372
BsaAI YACGTR 1 cut(s) 287
BsaJI CCNNGG 3 cut(s) 214, 374, 468
Bsc4I CCNNNNNNNGG 3 cut(s) 205, 460, 553
Bse3DI GCAATG 1 cut(s) 752
BseDI CCNNGG 3 cut(s) 214, 374, 468
BseGI GGATG 2 cut(s) 364, 785
BseLI CCNNNNNNNGG 3 cut(s) 205, 460, 553
BseMI GCAATG 1 cut(s) 752
BseMII CTCAG 1 cut(s) 543
BseRI GAGGAG 1 cut(s) 506
BseSI GKGCMC 2 cut(s) 649, 787
BseXI GCAGC 1 cut(s) 244
BshFI GGCC 1 cut(s) 691
BsiHKAI GWGCWC 3 cut(s) 396, 649, 787
BslFI GGGAC 1 cut(s) 821
BslI CCNNNNNNNGG 3 cut(s) 205, 460, 553
BsmFI GGGAC 1 cut(s) 821
BsmI GAATGC 1 cut(s) 215
BsnI GGCC 1 cut(s) 691
Bsp1286I GDGCHC 3 cut(s) 396, 649, 787
Bsp143I GATC 2 cut(s) 336, 810
Bsp19I CCATGG 1 cut(s) 468
BspANI GGCC 1 cut(s) 691
BspCNI CTCAG 1 cut(s) 544
BspHI TCATGA 1 cut(s) 16
BspLI GGNNCC 1 cut(s) 197
BsrDI GCAATG 1 cut(s) 752
BssECI CCNNGG 3 cut(s) 214, 374, 468
BssMI GATC 2 cut(s) 336, 810
BssNAI GTATAC 1 cut(s) 149
BssSI CACGAG 1 cut(s) 648
BssT1I CCWWGG 2 cut(s) 214, 468
Bst1107I GTATAC 1 cut(s) 149
Bst2BI CACGAG 1 cut(s) 648
Bst4CI ACNGT 3 cut(s) 347, 496, 858
BstBAI YACGTR 1 cut(s) 287
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 2 cut(s) 170, 552
BstDSI CCRYGG 2 cut(s) 374, 468
BstENI CCTNNNNNAGG 2 cut(s) 458, 551
BstF5I GGATG 2 cut(s) 364, 785
BstKTI GATC 2 cut(s) 339, 813
BstMBI GATC 2 cut(s) 336, 810
BstNSI RCATGY 2 cut(s) 603, 671
BstSLI GKGCMC 2 cut(s) 649, 787
BstSNI TACGTA 1 cut(s) 287
BstV1I GCAGC 1 cut(s) 244
BstV2I GAAGAC 2 cut(s) 554, 708
BstXI CCANNNNNNTGG 1 cut(s) 641
BstZ17I GTATAC 1 cut(s) 149
BsuRI GGCC 1 cut(s) 691
BtgI CCRYGG 2 cut(s) 374, 468
BtsCI GGATG 2 cut(s) 364, 785
BtsIMutI CAGTG 3 cut(s) 335, 433, 501
Cac8I GCNNGC 1 cut(s) 9
CciI TCATGA 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 195
Csp6I GTAC 1 cut(s) 761
CviAII CATG 8 cut(s) 17, 274, 469, 600, 641, 668, 764, 824
CviQI GTAC 1 cut(s) 761
DdeI CTNAG 2 cut(s) 170, 552
DpnI GATC 2 cut(s) 338, 812
DpnII GATC 2 cut(s) 336, 810
DrdI GACNNNNNNGTC 1 cut(s) 519
DseDI GACNNNNNNGTC 1 cut(s) 519
Ecl136II GAGCTC 1 cut(s) 394
Eco105I TACGTA 1 cut(s) 287
Eco130I CCWWGG 2 cut(s) 214, 468
Eco24I GRGCYC 1 cut(s) 396
Eco32I GATATC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 195
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 2 cut(s) 714, 720
EcoICRI GAGCTC 1 cut(s) 394
EcoNI CCTNNNNNAGG 2 cut(s) 458, 551
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 2 cut(s) 214, 468
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 2 cut(s) 214, 468
FaeI CATG 8 cut(s) 20, 277, 472, 603, 644, 671, 767, 827
FaqI GGGAC 1 cut(s) 821
FatI CATG 8 cut(s) 16, 273, 468, 599, 640, 667, 763, 823
FblI GTMKAC 1 cut(s) 148
Fnu4HI GCNGC 1 cut(s) 258
FokI GGATG 2 cut(s) 371, 792
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 1 cut(s) 258
FspBI CTAG 6 cut(s) 350, 369, 455, 623, 680, 758
GluI GCNGC 1 cut(s) 258
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 1 cut(s) 691
Hin1II CATG 8 cut(s) 20, 277, 472, 603, 644, 671, 767, 827
HinfI GANTC 4 cut(s) 67, 166, 235, 513
HphI GGTGA 1 cut(s) 375
Hpy166II GTNNAC 4 cut(s) 149, 501, 647, 785
Hpy188I TCNGA 4 cut(s) 52, 525, 544, 553
Hpy188III TCNNGA 5 cut(s) 17, 389, 419, 623, 680
Hpy8I GTNNAC 4 cut(s) 149, 501, 647, 785
HpyCH4III ACNGT 3 cut(s) 347, 496, 858
HpyCH4IV ACGT 2 cut(s) 246, 286
HpyCH4V TGCA 6 cut(s) 35, 314, 614, 647, 667, 785
HpyF3I CTNAG 2 cut(s) 170, 552
HpySE526I ACGT 2 cut(s) 246, 286
Hsp92II CATG 8 cut(s) 20, 277, 472, 603, 644, 671, 767, 827
Kzo9I GATC 2 cut(s) 336, 810
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 7 cut(s) 96, 128, 171, 213, 402, 705, 735
Lsp1109I GCAGC 1 cut(s) 244
MaeI CTAG 6 cut(s) 350, 369, 455, 623, 680, 758
MaeII ACGT 2 cut(s) 246, 286
MaeIII GTNAC 1 cut(s) 319
MalI GATC 2 cut(s) 338, 812
MboI GATC 2 cut(s) 336, 810
MboII GAAGA 3 cut(s) 346, 554, 713
MfeI CAATTG 1 cut(s) 315
MhlI GDGCHC 3 cut(s) 396, 649, 787
MluCI AATT 5 cut(s) 315, 363, 398, 576, 685
MlyI GAGTC 2 cut(s) 229, 507
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 8 cut(s) 57, 349, 396, 527, 547, 716, 827, 849
MseI TTAA 1 cut(s) 753
MslI CAYNNNNRTG 3 cut(s) 639, 790, 846
MspA1I CMGCKG 1 cut(s) 185
MunI CAATTG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 215
NcoI CCATGG 1 cut(s) 468
NdeII GATC 2 cut(s) 336, 810
NlaIII CATG 8 cut(s) 20, 277, 472, 603, 644, 671, 767, 827
NlaIV GGNNCC 1 cut(s) 197
NmuCI GTSAC 1 cut(s) 319
NspI RCATGY 2 cut(s) 603, 671
OliI CACNNNNGTG 1 cut(s) 790
PagI TCATGA 1 cut(s) 16
PctI GAATGC 1 cut(s) 215
PfeI GAWTC 2 cut(s) 67, 166
PkrI GCNGC 1 cut(s) 259
PleI GAGTC 2 cut(s) 229, 507
PpsI GAGTC 2 cut(s) 229, 507
Ppu21I YACGTR 1 cut(s) 287
Psp124BI GAGCTC 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 197
PspPI GGNCC 1 cut(s) 195
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 1 cut(s) 762
RsaNI GTAC 1 cut(s) 761
RseI CAYNNNNRTG 3 cut(s) 639, 790, 846
SacI GAGCTC 1 cut(s) 396
SaqAI TTAA 1 cut(s) 753
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 2 cut(s) 336, 810
Sau96I GGNCC 1 cut(s) 195
SchI GAGTC 2 cut(s) 229, 507
SduI GDGCHC 3 cut(s) 396, 649, 787
SinI GGWCC 1 cut(s) 195
SmiMI CAYNNNNRTG 3 cut(s) 639, 790, 846
SnaBI TACGTA 1 cut(s) 287
Sse9I AATT 5 cut(s) 315, 363, 398, 576, 685
SspMI CTAG 6 cut(s) 350, 369, 455, 623, 680, 758
SstI GAGCTC 1 cut(s) 396
StyI CCWWGG 2 cut(s) 214, 468
TaaI ACNGT 3 cut(s) 347, 496, 858
TaiI ACGT 2 cut(s) 249, 289
TaqI TCGA 1 cut(s) 131
TasI AATT 5 cut(s) 315, 363, 398, 576, 685
TatI WGTACW 1 cut(s) 760
TfiI GAWTC 2 cut(s) 67, 166
Tru1I TTAA 1 cut(s) 753
Tru9I TTAA 1 cut(s) 753
TscAI CASTG 3 cut(s) 335, 433, 501
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 1 cut(s) 257
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 2 cut(s) 5, 402
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 3 cut(s) 335, 433, 501
VneI GTGCAC 2 cut(s) 645, 783
VpaK11BI GGWCC 1 cut(s) 195
XagI CCTNNNNNAGG 2 cut(s) 458, 551
XapI RAATTY 1 cut(s) 398
XbaI TCTAGA 2 cut(s) 622, 679
XceI RCATGY 2 cut(s) 603, 671
XmiI GTMKAC 1 cut(s) 148
XspI CTAG 6 cut(s) 350, 369, 455, 623, 680, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.