MD00G1166400.v1.1

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000800
Physical Location & Seq
Reverse (-)
38145176 .. 38159952
14777 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1166400.v1.1.491

Sequence Viewer

Length: 5400 bp
ATGACAAATTTGCCCCTCGAACTCTTCGATATTTACCGGCTCTGCCTCGACTGCCTGGCCGCCGTGTCCTCTGTGTTGTCCGCTTCGCCGCACTCGTTTCACTACCCGAGGGTGAGGATGGTGAGTTGCCTTGTGGCATGTGGGCGGTACAAGGATGCGGAAAGTGAGGGGTTTAGGGTTTTGGAGAGCCTTAAGGCGATTGAATGTGGGTCGAAGAAATCGGTGAAATCGTGTCGGAGATTTGTGCCGGATGTGGAGAAGGGGGGCGGAGATAAAGATTTTGGGTCTTTGGTGGGTGAAATTGTGGTGACGCTTGTGAAATGTGCGGCCATGAGTCAGAGTAAGGACAGTGAGGTGTTTGAGCGGGTGCTTTGTTTGGCTGAGGAGGTCATGCCGTGGTTCCGGTGTACTCAATTTTTAGTTGGGGAGCTATCGGTGTTCAATGGGGATCTTGTGCAAAAGTTCTGCATTTTAACCATGACTGAGTATGCTAAGTCGCCAATGAAAAGACACAATGGTCAAGGTAGCCTATGTTTGATTCTATATCCTTTTCTTTTGTTCATACAGTTTGCCCGTACGATATGTTCATCTTTATTCTTGTTTCAAGAGGATACATTCACACTCACTCGCATCTTATTTTGCTTGTTGGACTCCCTTGTCCACGAATGTAAGGCTGAAGTGGAAAATTCAGGAAAAGAGTTTGTTGAACTTATTGCTTATTGTGCCAAAAAATGTCAAACTACAAACACAAACCTTTGCGGTATTATTGGATCTCATTTAAATAAGTTAGCAGGTGATTTCCATCAGGCTGGAACACCTTTTCAGTTAATTCTGAGGGTTTATGCCACTGGATTGCACTTTGTTGATCGTAGTATGAAGTCAAAAGTTGGTCATTTTCAATCCTTTGAAGGTGCAATTAGAGTTTTGCTTGATGATGGGGACACAGGGAATCGCTTGTCCGGTTTGCTTGGTTCATTGAGAAGTTATTTCCAAATTGGCTGCAATGACGACTCTTTGTTATCCAATTCGCAATTGAGTTCGGACAGTGGGGACTCATTGAATCAAATGCAGAAGGATAGAAAGAACTATCTTCTGTGTTATTTTAATGCATTGAAATTCTTATGCCAGCCGCTTACAGAGTTTGTAAATTCAGGAAGGAAACAGATTATTACTAATAATGAAGCTGCTTCTGTTTCTACTGAAGTGTGCCATATCCAGGGTGCATTCCATCAGTTTTGTGACGTTTTTCTTTCTCTTAAAATATGTACATATGAGGTGGATAGAGATGGATTTGATGGAAACAGCACCCTTGATGTTGCTCTGGCTGCTTTTACCCTCTCCATCATAACAAAGCTGAACATTCAGAAGAGTGTACAAATACTTGAAAATGTCATCACCAGTGCTTGGATTCAACCTCATGGTCTAAAGCATCTATATGTCTCTCTGTACAATACCGGGGTACATTTGTACAGGAACAAGGAGCTAAAAGAGGCATCACAGGCATTGAACTTATGTTGTAAGGCATCATGGACTCGTGTTATACATCTTTGTGAGATGTTTGTACACAAACAAAGGGCGTCCGAGGTTGATCTGTCAGAAGATGCCATTTTAGATTTCTATAATGAGTGCTGTACGAGAAGTGCTTTTCTTTTGGACGTTCTTAATGAATTGCAGAGTTATGATGCGAAAAGAACTTTATTAGAGAGCCTTGAAAATTGGTCCATTGCTGCAAACTTGTTTGGGAGGCTGCCAGGTCCTCTGGCTGTGGTGAAGCAGTGGGTTAAGATGGAATGTAAACGTTACAAGGATGTGAATGTTGAAGATGATGCTCCAACCTTATACAGTTTGTTATTGTCTTATAAGAAAGTCCCAAAGAAGATAAACGAAATCGTTTTGGAACAGGAACTTCTTGCTTATGAGGGAATGACTGCTGTGAACCCTAAGTTTTGTCAGAAAATGCAAATGAAAATTATAGATTTCCTGCTGAAAGATGTGTATGTCACACCAAATAGTTGGTTACAAAAATCAAGAATTTTGTTGAAAAAGGGAAGGGCATTAAGGCTTTCTGGAAGCAAAGGTCTGAAGGACTGTATTCAGTGTTTATCAGATGCAATATGTTTACTTAGTGAGATTTATGATGAAACCTGTACCCATGAAATTTCTCCTTGCCATCAATTAGCTGTGGCATATTGCTTACGTGCACTTTCTACCCAGGAAGCTGAACCCAACTCAAAGCGAGTGCTTGAAGATATCAGTGCTGCCATTAATCTATGGTTGGGCATTTCTACTCCTGCTAATTGCTCTCCGGCTGACAAGTGCTCCATGTTGTCTGAAAATACTATGTTGCTACTTTACAATGTCATTGATTTGTTATCCGCCAAGGGTTGCATGGACTTTCACAATGATATACATAAGCTTATGATTAGATTATTTAAATGGAGGAATGTCCCATTAGAGAAGTGCGTGGCCAGATTTTGGGAATGTAGGAGGATTAGCCATGCCCTTTGTGCCTCACCTGTAAATGAGACATTCATTATGAACTTATCAGATCATTGTGGTGAACTTTCAAAGTATGCTTTTTGGATAGATTCTCTAAAAGATTCCACACCATTACTATTAGTGTTCCAACATAGTTTCTCATTTCTGTTTCCAAACTTTTCTCGGGGCCCATGGAATCATCAAAATTTGTTTCGATCAGATATCACAATTGATGAAGTTAAGGAAGCTGCTTTTGAACTTATATCACAGGCTCCTGTATCGACTTGGTCTGCTTACATAGCTGGATATCTCTACTATGATTTATCGGAAAGACTTGTTTCAAATGGACGGTTAATTGAGGCTCTCTCATATGCAAAAGAAGCTCACAACTTACGTGCTAAACTATTTGGAGGGAAATTTATGTTCTCTTCTGAGCGACAGCCCAAAAAGTACAATGAAGGGGGTATCTGTCAGGAGTTAACATATAGCATTCATGATATGCATATGCAAAGATCAGTTGCTAGTGAAGTTTGGCTTTTTGATACTAGTTCATGTGACCTGGAAAGCTATTATCTGAGTCCATGGAATGCACTTCAATGTTATCTTGAGAGCACTCTTCAGGTAGGGGTTATCCTCGAAATAATTGGTAAAGGAGCTGAGGCCGAAGGTTTTTTACAATTTGGAAAAGCTTTTTCCTGCTCACAGAGCTTGCCACTATTTACAATTGTTTTTTCTACTGTCTTGGGAAAGCTTTACCACAAGCAGCAACTTTGGGATTTGGCGGAAAAGGAACTGCAAAGTGCCAAGCAATATTTTGGGGCTTGCAGCACAGATCTCTCTTGCATGAAATGCAGATTGTTGCTGGAAGCAACTGTTAATCAGAATCTTGGCGATTTATATCAAAGCATTTTTGAAAATACAAGAAGTACATCGTCAGATAAGTTGTCGCATGCTGAAAACCTGTACAAATCAGCCATTGCCATACTAAATCTTTCTGAGTGGAAAAATTCTGTTAGTTGTCCAGAAGAAGAGTGTGTCGAATGGACAATGCCTGGAAAAGCTAGTCTTAAAGACGTTGGATATTGTGCTAGCAGTATATATACTGTTTCTGAAGAAAAACAGCATGATAATAGAAAAACCACTAAGGAGGGCCTGAAAAGCAAGATGGATGCTAAAAAATGTAAAAAGACCAAAAATGCACCAAAACTGGTCGTGAAGAATCAGGTTTCAGTACCTGAGCACAATTTGAGGGTAACTCGGTCTAGATATCAATCTTCTCAGAACCAAAGCATAAGTGGAAATGGCATCGTCCAACTTGGTCCTTCAAAACTTCTGCAAGGAAAAAGTGAGTGCGATAGCCCTGATACTTTTAGCAAGAGGGAATTTCTATTGGATTTAAAAAGCTGTGAGGTTGCTTTTGGGTGCAATGTAACTTGCATTTGCAACCAAATGAGGTGTTGGCAGTGTCTTCCTGTGGAAGTTATGAAATCTGGGTTAGTGAAAGACTTAGTACATTTAAAATGGGAGTTTGTCCGCCGGCGTTTATTGCTCAGGCTTCTCACAGGATTAGGAAAATGCTTGGACAGTCGTGGTCAAACTCATGAGACACATGAAATTATAGTGCAAACTGTATCAGTCTTGGTCAGCCGAAACCCATTTTGTCCTATTACCTCAACTGTTCCATTGACTTCCTTGCTTGATTTAATGGGAAAGGAGATCCCCGGGGATGTGTTCTGTGTTGAACGGGCAGAAGTACTGTTGAACATAAGTTGGTCGTCTTTGAAGAGTTACTGTTCTAAGGAAACCAGGAGTATGTGCTCTGATCTGCCTCATATTCAGCTACCAAAACTAGTGTCTTGGTTGATGCTAGCTTTTGTTCTCTGCCGCGACGTTCCTGTACTTTTCCAGAAGGTTTCCAGATTGCTTGCCGCTATATTTGTGCTTTCTACCTCAAGTGATCTTTTTTCTTTGTCATCTTCTTCTAAAACTCTCCGCGAAAACCATTGGGCTTCTTATTTCCATCAAGCTTCACTTGGCACTCATCTTAGTTGCCAATTTTTCACAAATATATCTGGGATATGTAATGTTCAGCACCTTGTAAACACTGAGGGTTCACATGTTCCTGGCTCAACTTGTTTGGGATCAGAAAAAAAGAATTTACTCAGGCTTGCACCTGAGTCTATTCAAGAACTTGAAGGATTTGTAACACTGTTTTTTGCCGGCCTTCCTTGCACAACAATTATCTGTATAAGTTTGCTCGGAAGCCCTTATGCTAGTTTCCTACAGGAGCTGTTGTCCTTTCATACTTGTGTTCATGCATGGATTCTTGTATCACGCTTGAACTTGAAGAGTCAACCTATTGTTATGCTTCTACCTGTGGATTCAGTATTAGAAGGAGATTCCTCCGATGATACAAGTTCTGGTTCTGTTAGTGTTTCTGACGGAAAGGTTGGAAAGCGTTGGTGCTGCCCATGGGGTTCCACTGTGGTTGATAGGGTAGCTCCAGAATTTAGAATGATATTGGAGGAGAGTTATTTGTCATCTTCAATCGAAGAAGAAGAAGATACAAAAGAGAATAGGGCGTTATGGTGGATGATGTGGAGAAATAAGCTTGATCGTCGCCTCTGTAAATTGTTGAAGAACTTAGAAGATTTATGGTTTGGTCCTTGGAAATATTTGCTTCTGGGAGAATCGTCAAACTGCAAGCAGCTGGACTTGGTACATAAGAAGCTGGCGCGGGATTTGAAATCTAAGTGCAAAATGGACATAGATGAGAGTCTTCTGAAAGTCATTCTTGGGGGTTCCAAATATGCCTTTGAAGGTGGAGGCGCATATGTTTCACAGCTTTGTTTCAAGAAGTTTGTTTACATTCAGTTGTTATTTCATAAGTTGTGGATTCAATTCGTTCATCTATTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

1800

Amino Acids

202.96

Weight (kDa)

7.37

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 5 - 135 5.8e-49 Separase-like, TPR repeats
TPR_ESP1 PF25110 135 - 171 4.3e-09 Separase-like, TPR repeats
TPR_ESP1_2nd PF25113 210 - 389 5.5e-56 Separase-like, TPR repeats
Separin_C PF03568 1599 - 1763 2.3e-19 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1860
AarI CACCTGC 1 cut(s) 782
AasI GACNNNNNNGTC 2 cut(s) 516, 656
Acc36I ACCTGC 1 cut(s) 782
AccB7I CCANNNNNTGG 2 cut(s) 1404, 2475
AccBSI CCGCTC 1 cut(s) 364
AccII CGCG 3 cut(s) 4365, 4473, 5215
AclI AACGTT 1 cut(s) 1798
AclWI GGATC 4 cut(s) 456, 778, 4189, 4627
AcoI YGGCCR 3 cut(s) 57, 327, 2466
AcuI CTGAAG 5 cut(s) 696, 1221, 2102, 3080, 3609
AcyI GRCGYC 1 cut(s) 1577
AfiI CCNNNNNNNGG 3 cut(s) 1216, 1404, 2475
AflII CTTAAG 1 cut(s) 191
AflIII ACRYGT 1 cut(s) 4594
AhlI ACTAGT 2 cut(s) 3023, 4327
AjnI CCWGG 8 cut(s) 54, 1215, 1750, 2211, 3036, 3529, 4283, 4600
Alw21I GWGCWC 5 cut(s) 2203, 2321, 3092, 3720, 4298
Alw26I GTCTC 3 cut(s) 1444, 2519, 4076
Alw44I GTGCAC 1 cut(s) 2199
AlwI GGATC 4 cut(s) 456, 778, 4189, 4627
AlwNI CAGNNNCTG 2 cut(s) 3713, 4768
Ama87I CYCGRG 3 cut(s) 106, 2661, 4199
AoxI GGCC 7 cut(s) 57, 327, 2466, 2665, 3138, 3628, 4699
ApaI GGGCCC 1 cut(s) 2669
ApaLI GTGCAC 1 cut(s) 2199
ArsI GACNNNNNNTTYG 2 cut(s) 4316, 4348
AseI ATTAAT 1 cut(s) 2265
AspLEI GCGC 2 cut(s) 5215, 5309
AspS9I GGNCC 7 cut(s) 1719, 1754, 2665, 2666, 3628, 3797, 5141
AsuC2I CCSGG 3 cut(s) 1456, 4200, 4201
AsuNHI GCTAGC 2 cut(s) 3566, 4345
AvaI CYCGRG 3 cut(s) 106, 2661, 4199
AvaII GGWCC 4 cut(s) 1719, 1754, 3797, 5141
BaeGI GKGCMC 2 cut(s) 2203, 2669
BaeI ACNNNNGTAYC 2 cut(s) 1451, 1484
BalI TGGCCA 1 cut(s) 2468
BanII GRGCYC 1 cut(s) 2669
BarI GAAGNNNNNNTAC 4 cut(s) 3135, 3167, 5327, 5359
BauI CACGAG 1 cut(s) 1533
BbsI GAAGAC 2 cut(s) 3938, 5249
Bbv12I GWGCWC 5 cut(s) 2203, 2321, 3092, 3720, 4298
BbvCI CCTCAGC 2 cut(s) 381, 3135
BceAI ACGGC 2 cut(s) 47, 379
BciT130I CCWGG 8 cut(s) 56, 1217, 1752, 2213, 3038, 3531, 4285, 4602
BciVI GTATCC 1 cut(s) 604
BcnI CCSGG 3 cut(s) 1456, 4200, 4201
BcoDI GTCTC 3 cut(s) 1444, 2519, 4076
BcuI ACTAGT 2 cut(s) 3023, 4327
BfaI CTAG 8 cut(s) 3000, 3024, 3540, 3567, 3741, 4328, 4346, 4752
BfmI CTRYAG 1 cut(s) 4760
BfrI CTTAAG 1 cut(s) 191
BfuAI ACCTGC 1 cut(s) 782
BfuI GTATCC 1 cut(s) 604
BglII AGATCT 1 cut(s) 3310
BmcAI AGTACT 1 cut(s) 4233
Bme18I GGWCC 4 cut(s) 1719, 1754, 3797, 5141
BmeT110I CYCGRG 3 cut(s) 106, 2661, 4199
BmgT120I GGNCC 7 cut(s) 1719, 1754, 2665, 2666, 3628, 3797, 5141
BmiI GGNNCC 6 cut(s) 401, 2666, 2667, 2751, 4957, 5281
BmtI GCTAGC 2 cut(s) 3570, 4349
BpiI GAAGAC 2 cut(s) 3938, 5249
BplI GAGNNNNNCTC 4 cut(s) 2828, 2860, 3718, 3750
BpmI CTGGAG 1 cut(s) 4965
Bpu10I CCTNAGC 4 cut(s) 381, 3135, 3714, 4028
BpuEI CTTGAG 2 cut(s) 3104, 4414
BpuMI CCSGG 3 cut(s) 1456, 4200, 4201
BsaAI YACGTR 2 cut(s) 2198, 2873
BsaBI GATNNNNATC 3 cut(s) 801, 3375, 3748
BsaHI GRCGYC 1 cut(s) 1577
BsaWI WCCGGW 2 cut(s) 402, 959
BsaXI ACNNNNNCTCC 4 cut(s) 2303, 2333, 4757, 4787
Bsc4I CCNNNNNNNGG 3 cut(s) 1216, 1404, 2475
Bse118I RCCGGY 3 cut(s) 36, 4014, 4697
Bse1I ACTGG 3 cut(s) 853, 1398, 3690
Bse3DI GCAATG 4 cut(s) 1009, 1722, 3453, 3910
Bse8I GATNNNNATC 3 cut(s) 801, 3375, 3748
BseBI CCWGG 8 cut(s) 56, 1217, 1752, 2213, 3038, 3531, 4285, 4602
BseGI GGATG 7 cut(s) 123, 160, 256, 1813, 3652, 4210, 5076
BseJI GATNNNNATC 3 cut(s) 801, 3375, 3748
BseLI CCNNNNNNNGG 3 cut(s) 1216, 1404, 2475
BseMI GCAATG 4 cut(s) 1009, 1722, 3453, 3910
BseNI ACTGG 3 cut(s) 853, 1398, 3690
BseRI GAGGAG 2 cut(s) 398, 5018
BseSI GKGCMC 2 cut(s) 2203, 2669
Bsh1236I CGCG 3 cut(s) 4365, 4473, 5215
BshFI GGCC 7 cut(s) 59, 329, 2468, 2667, 3140, 3630, 4701
BsiHKAI GWGCWC 5 cut(s) 2203, 2321, 3092, 3720, 4298
BsiHKCI CYCGRG 3 cut(s) 106, 2661, 4199
BsiSI CCGG 9 cut(s) 37, 248, 403, 960, 1455, 2306, 4015, 4200, 4698
BsiWI CGTACG 1 cut(s) 575
BslFI GGGAC 4 cut(s) 953, 1064, 1853, 2432
BslI CCNNNNNNNGG 3 cut(s) 1216, 1404, 2475
BsmAI GTCTC 3 cut(s) 1444, 2519, 4076
BsmFI GGGAC 4 cut(s) 953, 1064, 1853, 2432
BsmI GAATGC 3 cut(s) 1223, 2967, 3070
BsnI GGCC 7 cut(s) 59, 329, 2468, 2667, 3140, 3630, 4701
BsoBI CYCGRG 3 cut(s) 106, 2661, 4199
Bsp120I GGGCCC 1 cut(s) 2665
Bsp1286I GDGCHC 6 cut(s) 2203, 2321, 2669, 3092, 3720, 4298
Bsp1407I TGTACA 6 cut(s) 1265, 1372, 1446, 1467, 1561, 3441
Bsp19I CCATGG 3 cut(s) 2669, 3059, 4949
BspANI GGCC 7 cut(s) 59, 329, 2468, 2667, 3140, 3630, 4701
BspFNI CGCG 3 cut(s) 4365, 4473, 5215
BspHI TCATGA 2 cut(s) 2971, 4078
BspLI GGNNCC 6 cut(s) 401, 2666, 2667, 2751, 4957, 5281
BspMI ACCTGC 1 cut(s) 782
BspOI GCTAGC 2 cut(s) 3570, 4349
BspPI GGATC 4 cut(s) 456, 778, 4189, 4627
BspTI CTTAAG 1 cut(s) 191
BsrBI CCGCTC 1 cut(s) 364
BsrDI GCAATG 4 cut(s) 1009, 1722, 3453, 3910
BsrFI RCCGGY 3 cut(s) 36, 4014, 4697
BsrGI TGTACA 6 cut(s) 1265, 1372, 1446, 1467, 1561, 3441
BsrI ACTGG 3 cut(s) 853, 1398, 3690
BssAI RCCGGY 3 cut(s) 36, 4014, 4697
BssNI GRCGYC 1 cut(s) 1577
BssSI CACGAG 1 cut(s) 1533
BssT1I CCWWGG 5 cut(s) 2379, 2669, 3059, 4949, 5144
Bst2BI CACGAG 1 cut(s) 1533
Bst2UI CCWGG 8 cut(s) 56, 1217, 1752, 2213, 3038, 3531, 4285, 4602
Bst6I CTCTTC 7 cut(s) 29, 1361, 2911, 3099, 3501, 4256, 4820
BstACI GRCGYC 1 cut(s) 1577
BstAFI CTTAAG 1 cut(s) 191
BstAPI GCANNNNNTGC 1 cut(s) 3327
BstAUI TGTACA 6 cut(s) 1265, 1372, 1446, 1467, 1561, 3441
BstBAI YACGTR 2 cut(s) 2198, 2873
BstDSI CCRYGG 4 cut(s) 395, 2669, 3059, 4949
BstF5I GGATG 7 cut(s) 123, 160, 256, 1813, 3652, 4210, 5076
BstFNI CGCG 3 cut(s) 4365, 4473, 5215
BstHHI GCGC 2 cut(s) 5215, 5309
BstMAI GTCTC 3 cut(s) 1444, 2519, 4076
BstNI CCWGG 8 cut(s) 56, 1217, 1752, 2213, 3038, 3531, 4285, 4602
BstNSI RCATGY 3 cut(s) 141, 3431, 4598
BstSFI CTRYAG 1 cut(s) 4760
BstSLI GKGCMC 2 cut(s) 2203, 2669
BstUI CGCG 3 cut(s) 4365, 4473, 5215
BstV2I GAAGAC 2 cut(s) 3938, 5249
BstX2I RGATCY 4 cut(s) 448, 770, 3310, 4194
BstXI CCANNNNNNTGG 2 cut(s) 809, 2013
BstYI RGATCY 4 cut(s) 448, 770, 3310, 4194
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 7 cut(s) 59, 329, 2468, 2667, 3140, 3630, 4701
BtgI CCRYGG 4 cut(s) 395, 2669, 3059, 4949
BtsCI GGATG 7 cut(s) 123, 160, 256, 1813, 3652, 4210, 5076
BtsI GCAGTG 2 cut(s) 1781, 3947
BveI ACCTGC 1 cut(s) 782
CaiI CAGNNNCTG 2 cut(s) 3713, 4768
CciI TCATGA 2 cut(s) 2971, 4078
CfoI GCGC 2 cut(s) 5215, 5309
Cfr10I RCCGGY 3 cut(s) 36, 4014, 4697
Cfr13I GGNCC 7 cut(s) 1719, 1754, 2665, 2666, 3628, 3797, 5141
Cfr9I CCCGGG 1 cut(s) 4199
CseI GACGC 2 cut(s) 319, 1566
CspCI CAANNNNNGTGG 2 cut(s) 4948, 4983
DraI TTTAAA 4 cut(s) 780, 2434, 3876, 3996
DrdI GACNNNNNNGTC 2 cut(s) 516, 656
DseDI GACNNNNNNGTC 2 cut(s) 516, 656
EaeI YGGCCR 3 cut(s) 57, 327, 2466
Eam1104I CTCTTC 7 cut(s) 29, 1361, 2911, 3099, 3501, 4256, 4820
EarI CTCTTC 7 cut(s) 29, 1361, 2911, 3099, 3501, 4256, 4820
EciI GGCGGA 4 cut(s) 282, 2365, 3275, 4001
Eco130I CCWWGG 5 cut(s) 2379, 2669, 3059, 4949, 5144
Eco24I GRGCYC 1 cut(s) 2669
Eco32I GATATC 4 cut(s) 2251, 2701, 2786, 3746
Eco47I GGWCC 4 cut(s) 1719, 1754, 3797, 5141
Eco57I CTGAAG 5 cut(s) 696, 1221, 2102, 3080, 3609
Eco88I CYCGRG 3 cut(s) 106, 2661, 4199
EcoO109I RGGNCCY 3 cut(s) 1754, 2665, 3628
EcoRII CCWGG 8 cut(s) 54, 1215, 1750, 2211, 3036, 3529, 4283, 4600
EcoRV GATATC 4 cut(s) 2251, 2701, 2786, 3746
EcoT14I CCWWGG 5 cut(s) 2379, 2669, 3059, 4949, 5144
EcoT22I ATGCAT 3 cut(s) 1111, 2982, 4798
EcoT38I GRGCYC 1 cut(s) 2669
ErhI CCWWGG 5 cut(s) 2379, 2669, 3059, 4949, 5144
FalI AAGNNNNNCTT 8 cut(s) 2997, 3029, 3528, 3560, 4494, 4526, 5256, 5288
FaqI GGGAC 4 cut(s) 953, 1064, 1853, 2432
FauI CCCGC 2 cut(s) 357, 5208
FauNDI CATATG 4 cut(s) 1270, 2848, 2982, 5311
FokI GGATG 7 cut(s) 130, 167, 263, 1820, 3659, 4217, 5083
FriOI GRGCYC 1 cut(s) 2669
FspBI CTAG 8 cut(s) 3000, 3024, 3540, 3567, 3741, 4328, 4346, 4752
GlaI GCGC 2 cut(s) 5214, 5308
GsuI CTGGAG 1 cut(s) 4965
HaeIII GGCC 7 cut(s) 59, 329, 2468, 2667, 3140, 3630, 4701
HapII CCGG 9 cut(s) 37, 248, 403, 960, 1455, 2306, 4015, 4200, 4698
HgaI GACGC 2 cut(s) 319, 1566
HhaI GCGC 2 cut(s) 5215, 5309
Hin1I GRCGYC 1 cut(s) 1577
Hin6I GCGC 2 cut(s) 5213, 5307
HinP1I GCGC 2 cut(s) 5213, 5307
HincII GTYRAC 2 cut(s) 2958, 4832
HindII GTYRAC 2 cut(s) 2958, 4832
HindIII AAGCTT 5 cut(s) 2414, 3165, 3227, 4503, 5087
HpaI GTTAAC 1 cut(s) 2958
HpaII CCGG 9 cut(s) 37, 248, 403, 960, 1455, 2306, 4015, 4200, 4698
Hpy99I CGWCG 2 cut(s) 4370, 5100
HpyCH4IV ACGT 7 cut(s) 1242, 1656, 1798, 2197, 2872, 3552, 4368
HpySE526I ACGT 7 cut(s) 1242, 1656, 1798, 2197, 2872, 3552, 4368
Hsp92I GRCGYC 1 cut(s) 1577
HspAI GCGC 2 cut(s) 5213, 5307
KroI GCCGGC 2 cut(s) 4014, 4697
KroNI GCCGGC 2 cut(s) 4016, 4699
KspAI GTTAAC 1 cut(s) 2958
LmnI GCTCC 8 cut(s) 427, 1480, 1834, 2324, 2755, 3131, 4765, 4984
MaeI CTAG 8 cut(s) 3000, 3024, 3540, 3567, 3741, 4328, 4346, 4752
MaeII ACGT 7 cut(s) 1242, 1656, 1798, 2197, 2872, 3552, 4368
MbiI CCGCTC 1 cut(s) 364
MfeI CAATTG 3 cut(s) 1031, 2706, 3201
MflI RGATCY 4 cut(s) 448, 770, 3310, 4194
MhlI GDGCHC 6 cut(s) 2203, 2321, 2669, 3092, 3720, 4298
MlsI TGGCCA 1 cut(s) 2468
MluNI TGGCCA 1 cut(s) 2468
MlyI GAGTC 9 cut(s) 343, 644, 1004, 1046, 1525, 3064, 4664, 4837, 5263
MmeI TCCRAC 7 cut(s) 215, 627, 1856, 2650, 3535, 3814, 4909
Mox20I TGGCCA 1 cut(s) 2468
Mph1103I ATGCAT 3 cut(s) 1111, 2982, 4798
MreI CGCCGGCG 1 cut(s) 4014
MroNI GCCGGC 2 cut(s) 4014, 4697
MscI TGGCCA 1 cut(s) 2468
MslI CAYNNNNRTG 5 cut(s) 1434, 1548, 2556, 3073, 4785
Msp20I TGGCCA 1 cut(s) 2468
MspA1I CMGCKG 1 cut(s) 5188
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 9 cut(s) 37, 248, 403, 960, 1455, 2306, 4015, 4200, 4698
MunI CAATTG 3 cut(s) 1031, 2706, 3201
Mva1269I GAATGC 3 cut(s) 1223, 2967, 3070
MvaI CCWGG 8 cut(s) 56, 1217, 1752, 2213, 3038, 3531, 4285, 4602
MvnI CGCG 3 cut(s) 4365, 4473, 5215
NaeI GCCGGC 2 cut(s) 4016, 4699
NciI CCSGG 3 cut(s) 1456, 4200, 4201
NcoI CCATGG 3 cut(s) 2669, 3059, 4949
NdeI CATATG 4 cut(s) 1270, 2848, 2982, 5311
NgoMIV GCCGGC 2 cut(s) 4014, 4697
NheI GCTAGC 2 cut(s) 3566, 4345
NlaIV GGNNCC 6 cut(s) 401, 2666, 2667, 2751, 4957, 5281
NmuCI GTSAC 4 cut(s) 307, 1238, 1999, 3032
NsiI ATGCAT 3 cut(s) 1111, 2982, 4798
NspI RCATGY 3 cut(s) 141, 3431, 4598
PaeI GCATGC 1 cut(s) 3431
PagI TCATGA 2 cut(s) 2971, 4078
PaqCI CACCTGC 1 cut(s) 782
PciI ACATGT 1 cut(s) 4594
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 92, 227
PctI GAATGC 3 cut(s) 1223, 2967, 3070
PdiI GCCGGC 2 cut(s) 4016, 4699
Pfl23II CGTACG 1 cut(s) 575
PflFI GACNNNGTC 1 cut(s) 2764
PflMI CCANNNNNTGG 2 cut(s) 1404, 2475
PleI GAGTC 9 cut(s) 342, 644, 1004, 1046, 1525, 3063, 4663, 4836, 5262
PpsI GAGTC 9 cut(s) 342, 644, 1004, 1046, 1525, 3063, 4663, 4836, 5262
Ppu21I YACGTR 2 cut(s) 2198, 2873
PpuMI RGGWCCY 1 cut(s) 1754
PscI ACATGT 1 cut(s) 4594
PshBI ATTAAT 1 cut(s) 2265
PsiI TTATAA 1 cut(s) 1860
Psp1406I AACGTT 1 cut(s) 1798
Psp5II RGGWCCY 1 cut(s) 1754
Psp6I CCWGG 8 cut(s) 54, 1215, 1750, 2211, 3036, 3529, 4283, 4600
PspGI CCWGG 8 cut(s) 54, 1215, 1750, 2211, 3036, 3529, 4283, 4600
PspLI CGTACG 1 cut(s) 575
PspN4I GGNNCC 6 cut(s) 401, 2666, 2667, 2751, 4957, 5281
PspOMI GGGCCC 1 cut(s) 2665
PspPI GGNCC 7 cut(s) 1719, 1754, 2665, 2666, 3628, 3797, 5141
PspPPI RGGWCCY 1 cut(s) 1754
PsrI GAACNNNNNNTAC 2 cut(s) 568, 600
PstNI CAGNNNCTG 2 cut(s) 3713, 4768
PsuI RGATCY 4 cut(s) 448, 770, 3310, 4194
PsyI GACNNNGTC 1 cut(s) 2764
PvuII CAGCTG 1 cut(s) 5188
RseI CAYNNNNRTG 5 cut(s) 1434, 1548, 2556, 3073, 4785
Sau96I GGNCC 7 cut(s) 1719, 1754, 2665, 2666, 3628, 3797, 5141
ScaI AGTACT 1 cut(s) 4233
SchI GAGTC 9 cut(s) 343, 644, 1004, 1046, 1525, 3064, 4664, 4837, 5263
SduI GDGCHC 6 cut(s) 2203, 2321, 2669, 3092, 3720, 4298
SfcI CTRYAG 1 cut(s) 4760
SgrAI CRCCGGYG 1 cut(s) 4014
SinI GGWCC 4 cut(s) 1719, 1754, 3797, 5141
SmaI CCCGGG 1 cut(s) 4201
SmiI ATTTAAAT 2 cut(s) 780, 2434
SmiMI CAYNNNNRTG 5 cut(s) 1434, 1548, 2556, 3073, 4785
SmlI CTYRAG 3 cut(s) 191, 3083, 4429
SmoI CTYRAG 3 cut(s) 191, 3083, 4429
SpeI ACTAGT 2 cut(s) 3023, 4327
SphI GCATGC 1 cut(s) 3431
SspI AATATT 2 cut(s) 3290, 5153
SspMI CTAG 8 cut(s) 3000, 3024, 3540, 3567, 3741, 4328, 4346, 4752
StyI CCWWGG 5 cut(s) 2379, 2669, 3059, 4949, 5144
SwaI ATTTAAAT 2 cut(s) 780, 2434
TaiI ACGT 7 cut(s) 1245, 1659, 1801, 2200, 2875, 3555, 4371
TaqI TCGA 9 cut(s) 18, 27, 48, 212, 2692, 2759, 3114, 3516, 5028
TaqII GACCGA 1 cut(s) 3726
TauI GCSGC 6 cut(s) 62, 91, 329, 1132, 4365, 4409
TseFI GTSAC 4 cut(s) 307, 1238, 1999, 3032
Tsp45I GTSAC 4 cut(s) 307, 1238, 1999, 3032
TspGWI ACGGA 1 cut(s) 4935
TspMI CCCGGG 1 cut(s) 4199
Tth111I GACNNNGTC 1 cut(s) 2764
Van91I CCANNNNNTGG 2 cut(s) 1404, 2475
Vha464I CTTAAG 1 cut(s) 191
VneI GTGCAC 1 cut(s) 2199
VpaK11BI GGWCC 4 cut(s) 1719, 1754, 3797, 5141
VspI ATTAAT 1 cut(s) 2265
XbaI TCTAGA 1 cut(s) 3740
XceI RCATGY 3 cut(s) 141, 3431, 4598
XcmI CCANNNNNNNNNTGG 2 cut(s) 2386, 3770
XmaI CCCGGG 1 cut(s) 4199
XspI CTAG 8 cut(s) 3000, 3024, 3540, 3567, 3741, 4328, 4346, 4752
ZrmI AGTACT 1 cut(s) 4233
Zsp2I ATGCAT 3 cut(s) 1111, 2982, 4798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.