FvH4_5g29932

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000800
Physical Location & Seq
Reverse (-)
20807407 .. 20808139
733 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29932.t1

Sequence Viewer

Length: 657 bp
ATGGCCTCCGCCGCGGAGACCTCCGAAACTCTCCTCTCCAAGCTCGAATCCGCCGATTCTGCCGGGACTCACAGCCTCATCTCCGACTACCTAAATCCATTCGCCGACCTTCACAACAAAAAGAAGAAGAAGACCTCCGCCGACCTCCAAACCCTACAACGCTCTCTCGCCAAAAAATTCACCCCCTTCCTCAACCTCGCCTTAGCCATCCTCCCCAAACGCCTCGCCGATCCTTCAAACCTCGATGCCGATTTTGCCCCCGAATTGTTCGACATTTACCGCCTCTGCCTCGACTCCATGGACGCCGTGTCATCGCAATTCTCCGGCAAGCCATACAAGCTCCACATCCAGAGGATCCGGTTGGTTTGCGCCTTCGAGAAGTGGGGCCGCCACTGGGACGCCGAGTCCGAGGCGCTTAGGGTTTTGAAGAGCTTCAAGGCGATTCAATTAGGAAAGGAGCCGGTGGATTTGGACCGTCGATTTGTGCCAGGTGTCGAGCAGGGCGGCGCTGATATCGGAGAAGTGGTGTATATTCTAGCGAGGTGTGCTTCTGCGATTCGAAGCAAGGAAGAGGAGGTGTATGAGCGGCTGCTTTGTATGGTTGAGGAGTTGGCTCCTTGGTTTAGGTTTGAAATTGCGAGTGCATTGCCGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

219

Amino Acids

24.48

Weight (kDa)

6.45

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 9 - 210 3.5e-66 Separase-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 586
AccII CGCG 1 cut(s) 14
AciI CCGC 9 cut(s) 9, 12, 14, 51, 138, 280, 388, 504, 586
AclWI GGATC 3 cut(s) 224, 349, 362
AcsI RAATTY 1 cut(s) 176
AcyI GRCGYC 2 cut(s) 303, 399
AfiI CCNNNNNNNGG 1 cut(s) 394
AgsI TTSAA 5 cut(s) 237, 427, 436, 446, 632
AhdI GACNNNNNGTC 2 cut(s) 307, 403
AjnI CCWGG 1 cut(s) 487
AluBI AGCT 3 cut(s) 43, 340, 432
AluI AGCT 3 cut(s) 43, 340, 432
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 3 cut(s) 224, 349, 362
AoxI GGCC 2 cut(s) 3, 385
ApeKI GCWGC 1 cut(s) 589
ApoI RAATTY 1 cut(s) 176
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
Asp700I GAANNNNTTC 1 cut(s) 431
AspLEI GCGC 3 cut(s) 371, 415, 509
AspS9I GGNCC 2 cut(s) 385, 472
AsuC2I CCSGG 1 cut(s) 64
AsuHPI GGTGA 1 cut(s) 172
AsuII TTCGAA 1 cut(s) 559
AvaII GGWCC 1 cut(s) 472
BamHI GGATCC 1 cut(s) 354
BbsI GAAGAC 1 cut(s) 137
BbvI GCAGC 1 cut(s) 576
BccI CCATC 1 cut(s) 215
BceAI ACGGC 1 cut(s) 290
BcgI CGANNNNNNTGC 1 cut(s) 628
BciT130I CCWGG 1 cut(s) 489
BcnI CCSGG 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 1 cut(s) 536
BfoI RGCGCY 2 cut(s) 416, 510
BisI GCNGC 5 cut(s) 12, 388, 505, 587, 590
BlsI GCNGC 5 cut(s) 13, 389, 506, 588, 591
Bme1390I CCNGG 2 cut(s) 64, 489
Bme18I GGWCC 1 cut(s) 472
BmeRI GACNNNNNGTC 2 cut(s) 307, 403
BmgT120I GGNCC 2 cut(s) 385, 472
BmiI GGNNCC 4 cut(s) 356, 386, 459, 615
BmrFI CCNGG 2 cut(s) 64, 489
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 1 cut(s) 235
BmuI ACTGGG 1 cut(s) 403
BpiI GAAGAC 1 cut(s) 137
Bpu10I CCTNAGC 2 cut(s) 202, 416
Bpu14I TTCGAA 1 cut(s) 559
BpuMI CCSGG 1 cut(s) 64
BsaHI GRCGYC 2 cut(s) 303, 399
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 4 cut(s) 12, 297, 408, 617
BsaWI WCCGGW 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 398
Bse3DI GCAATG 1 cut(s) 644
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 4 cut(s) 12, 297, 408, 617
BseGI GGATG 2 cut(s) 207, 345
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMI GCAATG 1 cut(s) 644
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 3 cut(s) 23, 587, 620
BseXI GCAGC 1 cut(s) 576
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 2 cut(s) 5, 387
BsiSI CCGG 4 cut(s) 63, 324, 358, 461
BslFI GGGAC 2 cut(s) 79, 410
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 1 cut(s) 11
BsmFI GGGAC 2 cut(s) 79, 410
BsnI GGCC 2 cut(s) 5, 387
Bso31I GGTCTC 1 cut(s) 11
Bsp119I TTCGAA 1 cut(s) 559
Bsp143I GATC 2 cut(s) 229, 354
Bsp19I CCATGG 1 cut(s) 297
BspACI CCGC 9 cut(s) 9, 12, 14, 51, 138, 280, 388, 504, 586
BspANI GGCC 2 cut(s) 5, 387
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 4 cut(s) 356, 386, 459, 615
BspPI GGATC 3 cut(s) 224, 349, 362
BspQI GCTCTTC 1 cut(s) 422
BspT104I TTCGAA 1 cut(s) 559
BspTNI GGTCTC 1 cut(s) 11
BsrBI CCGCTC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 644
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 1 cut(s) 398
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 4 cut(s) 12, 297, 408, 617
BssMI GATC 2 cut(s) 229, 354
BssNI GRCGYC 2 cut(s) 303, 399
BssT1I CCWWGG 2 cut(s) 297, 617
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 1 cut(s) 476
Bst6I CTCTTC 2 cut(s) 422, 564
BstACI GRCGYC 2 cut(s) 303, 399
BstBI TTCGAA 1 cut(s) 559
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 2 cut(s) 202, 416
BstDSI CCRYGG 2 cut(s) 12, 297
BstF5I GGATG 2 cut(s) 207, 345
BstFNI CGCG 1 cut(s) 14
BstH2I RGCGCY 2 cut(s) 416, 510
BstHHI GCGC 3 cut(s) 371, 415, 509
BstKTI GATC 2 cut(s) 232, 357
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 2 cut(s) 229, 354
BstMWI GCNNNNNNNGC 5 cut(s) 11, 59, 254, 337, 545
BstNI CCWGG 1 cut(s) 489
BstSCI CCNGG 2 cut(s) 62, 487
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 576
BstV2I GAAGAC 1 cut(s) 137
BstX2I RGATCY 1 cut(s) 354
BstYI RGATCY 1 cut(s) 354
BsuRI GGCC 2 cut(s) 5, 387
BtgI CCRYGG 2 cut(s) 12, 297
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 2 cut(s) 207, 345
BtsIMutI CAGTG 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 329
CfoI GCGC 3 cut(s) 371, 415, 509
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 385, 472
Cfr42I CCGCGG 1 cut(s) 15
CseI GACGC 2 cut(s) 311, 407
CviAII CATG 1 cut(s) 298
DdeI CTNAG 2 cut(s) 202, 416
DpnI GATC 2 cut(s) 231, 356
DpnII GATC 2 cut(s) 229, 354
DriI GACNNNNNGTC 2 cut(s) 307, 403
Eam1104I CTCTTC 2 cut(s) 422, 564
Eam1105I GACNNNNNGTC 2 cut(s) 307, 403
EarI CTCTTC 2 cut(s) 422, 564
EciI GGCGGA 2 cut(s) 40, 127
Eco130I CCWWGG 2 cut(s) 297, 617
Eco31I GGTCTC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 472
EcoRII CCWGG 1 cut(s) 487
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 2 cut(s) 297, 617
ErhI CCWWGG 2 cut(s) 297, 617
FaeI CATG 1 cut(s) 301
FaiI YATR 5 cut(s) 299, 334, 531, 582, 599
FaqI GGGAC 2 cut(s) 79, 410
FatI CATG 1 cut(s) 297
Fnu4HI GCNGC 5 cut(s) 12, 388, 505, 587, 590
FokI GGATG 2 cut(s) 194, 332
Fsp4HI GCNGC 5 cut(s) 12, 388, 505, 587, 590
FspBI CTAG 1 cut(s) 536
GlaI GCGC 3 cut(s) 370, 414, 508
GluI GCNGC 5 cut(s) 12, 388, 505, 587, 590
HaeII RGCGCY 2 cut(s) 416, 510
HaeIII GGCC 2 cut(s) 5, 387
HapII CCGG 4 cut(s) 63, 324, 358, 461
HgaI GACGC 2 cut(s) 311, 407
HhaI GCGC 3 cut(s) 371, 415, 509
Hin1I GRCGYC 2 cut(s) 303, 399
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 3 cut(s) 369, 413, 507
HinP1I GCGC 3 cut(s) 369, 413, 507
HinfI GANTC 7 cut(s) 47, 56, 67, 293, 404, 442, 556
HpaII CCGG 4 cut(s) 63, 324, 358, 461
HphI GGTGA 1 cut(s) 172
Hpy188I TCNGA 4 cut(s) 25, 85, 409, 518
Hpy188III TCNNGA 2 cut(s) 349, 376
Hpy99I CGWCG 1 cut(s) 480
HpyAV CCTTC 4 cut(s) 119, 196, 243, 382
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4V TGCA 1 cut(s) 644
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 59, 254, 337, 545
HpyF3I CTNAG 2 cut(s) 202, 416
Hsp92I GRCGYC 2 cut(s) 303, 399
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 3 cut(s) 369, 413, 507
KspI CCGCGG 1 cut(s) 15
Kzo9I GATC 2 cut(s) 229, 354
LguI GCTCTTC 1 cut(s) 422
LmnI GCTCC 3 cut(s) 345, 457, 619
LpnPI CCDG 9 cut(s) 76, 337, 362, 371, 379, 474, 474, 485, 501
Lsp1109I GCAGC 1 cut(s) 576
LweI GCATC 1 cut(s) 235
MaeI CTAG 1 cut(s) 536
MalI GATC 2 cut(s) 231, 356
MbiI CCGCTC 1 cut(s) 586
MboI GATC 2 cut(s) 229, 354
MboII GAAGA 5 cut(s) 136, 139, 142, 439, 581
MflI RGATCY 1 cut(s) 354
MluCI AATT 5 cut(s) 176, 263, 317, 446, 633
MlyI GAGTC 3 cut(s) 61, 287, 413
MmeI TCCRAC 1 cut(s) 108
MroXI GAANNNNTTC 1 cut(s) 431
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 4 cut(s) 63, 324, 358, 461
MspR9I CCNGG 2 cut(s) 64, 489
MvaI CCWGG 1 cut(s) 489
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 5 cut(s) 11, 59, 254, 337, 545
NciI CCSGG 1 cut(s) 64
NcoI CCATGG 1 cut(s) 297
NdeII GATC 2 cut(s) 229, 354
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 4 cut(s) 356, 386, 459, 615
NmeAIII GCCGAG 1 cut(s) 427
NspV TTCGAA 1 cut(s) 559
PciSI GCTCTTC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 405
PdmI GAANNNNTTC 1 cut(s) 431
PfeI GAWTC 4 cut(s) 47, 56, 442, 556
PkrI GCNGC 5 cut(s) 13, 389, 506, 588, 591
PleI GAGTC 3 cut(s) 61, 287, 412
PpsI GAGTC 3 cut(s) 61, 287, 412
Psp6I CCWGG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 4 cut(s) 356, 386, 459, 615
PspPI GGNCC 2 cut(s) 385, 472
PsuI RGATCY 1 cut(s) 354
SacII CCGCGG 1 cut(s) 15
SapI GCTCTTC 1 cut(s) 422
SatI GCNGC 5 cut(s) 12, 388, 505, 587, 590
Sau3AI GATC 2 cut(s) 229, 354
Sau96I GGNCC 2 cut(s) 385, 472
SchI GAGTC 3 cut(s) 61, 287, 413
ScrFI CCNGG 2 cut(s) 64, 489
SfaNI GCATC 1 cut(s) 235
Sfr303I CCGCGG 1 cut(s) 15
SfuI TTCGAA 1 cut(s) 559
SgrBI CCGCGG 1 cut(s) 15
SinI GGWCC 1 cut(s) 472
Sse9I AATT 5 cut(s) 176, 263, 317, 446, 633
SsiI CCGC 9 cut(s) 9, 12, 14, 51, 138, 280, 388, 504, 586
SspMI CTAG 1 cut(s) 536
StyD4I CCNGG 2 cut(s) 62, 487
StyI CCWWGG 2 cut(s) 297, 617
TaaI ACNGT 1 cut(s) 476
TaqI TCGA 8 cut(s) 45, 243, 270, 291, 375, 478, 495, 559
TasI AATT 5 cut(s) 176, 263, 317, 446, 633
TauI GCSGC 4 cut(s) 14, 390, 507, 589
TfiI GAWTC 4 cut(s) 47, 56, 442, 556
TscAI CASTG 1 cut(s) 398
TseI GCWGC 1 cut(s) 589
TspRI CASTG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 472
XapI RAATTY 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.