MD08G1226700.v1.1

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000800
Physical Location & Seq
Forward (+)
29306911 .. 29308055
1145 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1226700.v1.1.491

Sequence Viewer

Length: 678 bp
ATGCTTCCTTGGGAGAATATACCAATACTAAGAAATCAGGAGGCTTATCGCATGCCTTGTGTTTGGAGCATCTTTGCAACACTGGAGGAGAACTACCATCAAGATCAAGTTGTGAGTAATACTATGAAACCAGGACGCTTATCGTATGCCCTCTGTCAGGAGGCTTCATTCCCTTTGATTGATCCATTGGATGCATTTTATCTCCTGAACCCAGGTGGTGATCTGGGTACCACACAAATCGAATCTGAGAAGTGGTTTAGAGATCAAAATTTGGAGGGGAAGGCTGGATGTGCACCCCCAGCTGAAGAATTGGCTGAAGCCTTGAAAAGCCACAACCTCTTTTTATATATCGGCCATGGAAGTGACAAGGATATTAACCAATTTGCAAAGGCCATGCTGGATGGTTGGCTAGAAGAAAGATCGAGTTCCTCTCAGGGTTGTGCCCAATGCAAAGTAGCAGAAGAAATTGAGGACTTGAGCATTAGGGGTCGTCAAGGTAATGCCAACAAAGTCTGGAGAAAGAAATTGCCTGAAGCTTGTGGAAGTGATCCAACGATGATTTCTTGTGATCAAAGGTCGAAGATTGGATCATTCACGAGTCAGGCTCGTGAAGCTTGCACCCTCCCTTTCTTGATTGGAGCATCACCGGTATGTTATGGTGTTCCTGTTGGTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

226

Amino Acids

24.94

Weight (kDa)

4.98

Isoelectric Point (pI)

56.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Separin_C PF03568 1 - 121 2.3e-25 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 227
AccB1I GGYRCC 1 cut(s) 227
AclWI GGATC 3 cut(s) 176, 542, 595
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 1 cut(s) 268
AcuI CTGAAG 3 cut(s) 324, 336, 552
AfaI GTAC 1 cut(s) 229
AfiI CCNNNNNNNGG 1 cut(s) 157
AgeI ACCGGT 1 cut(s) 646
AgsI TTSAA 1 cut(s) 325
AjnI CCWGG 2 cut(s) 130, 211
AjuI GAANNNNNNNTTGG 2 cut(s) 544, 576
AluBI AGCT 3 cut(s) 302, 536, 614
AluI AGCT 3 cut(s) 302, 536, 614
Alw21I GWGCWC 1 cut(s) 295
Alw44I GTGCAC 1 cut(s) 291
AlwI GGATC 3 cut(s) 176, 542, 595
AoxI GGCC 2 cut(s) 352, 390
ApaLI GTGCAC 1 cut(s) 291
ApoI RAATTY 1 cut(s) 268
AsiGI ACCGGT 1 cut(s) 646
Asp718I GGTACC 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 230, 636
BaeGI GKGCMC 2 cut(s) 295, 445
BanI GGYRCC 1 cut(s) 227
BauI CACGAG 2 cut(s) 595, 606
Bbv12I GWGCWC 1 cut(s) 295
BccI CCATC 2 cut(s) 105, 395
BciT130I CCWGG 2 cut(s) 132, 213
BclI TGATCA 1 cut(s) 568
BfaI CTAG 1 cut(s) 410
Bme1390I CCNGG 2 cut(s) 132, 213
BmiI GGNNCC 1 cut(s) 229
BmrFI CCNGG 2 cut(s) 132, 213
BmsI GCATC 3 cut(s) 78, 181, 650
BplI GAGNNNNNCTC 4 cut(s) 415, 447, 589, 621
BpmI CTGGAG 2 cut(s) 104, 535
BpuEI CTTGAG 1 cut(s) 496
BsaJI CCNNGG 3 cut(s) 8, 211, 355
BsaWI WCCGGW 1 cut(s) 646
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse118I RCCGGY 1 cut(s) 646
Bse1I ACTGG 1 cut(s) 87
BseBI CCWGG 2 cut(s) 132, 213
BseDI CCNNGG 3 cut(s) 8, 211, 355
BseGI GGATG 3 cut(s) 196, 293, 406
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 237, 446
BseNI ACTGG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 2 cut(s) 295, 445
BseYI CCCAGC 1 cut(s) 298
BshFI GGCC 2 cut(s) 354, 392
BshNI GGYRCC 1 cut(s) 227
BshTI ACCGGT 1 cut(s) 646
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 1 cut(s) 647
BslI CCNNNNNNNGG 1 cut(s) 157
BsnI GGCC 2 cut(s) 354, 392
Bsp1286I GDGCHC 2 cut(s) 295, 445
Bsp143I GATC 8 cut(s) 103, 181, 220, 262, 419, 547, 568, 587
Bsp19I CCATGG 1 cut(s) 355
BspANI GGCC 2 cut(s) 354, 392
BspCNI CTCAG 2 cut(s) 238, 445
BspLI GGNNCC 1 cut(s) 229
BspPI GGATC 3 cut(s) 176, 542, 595
BspT107I GGYRCC 1 cut(s) 227
BsrFI RCCGGY 1 cut(s) 646
BsrI ACTGG 1 cut(s) 87
BssAI RCCGGY 1 cut(s) 646
BssECI CCNNGG 3 cut(s) 8, 211, 355
BssMI GATC 8 cut(s) 103, 181, 220, 262, 419, 547, 568, 587
BssSI CACGAG 2 cut(s) 595, 606
BssT1I CCWWGG 2 cut(s) 8, 355
Bst2BI CACGAG 2 cut(s) 595, 606
Bst2UI CCWGG 2 cut(s) 132, 213
BstC8I GCNNGC 2 cut(s) 53, 616
BstDEI CTNAG 3 cut(s) 29, 246, 432
BstDSI CCRYGG 1 cut(s) 355
BstENI CCTNNNNNAGG 1 cut(s) 155
BstF5I GGATG 3 cut(s) 196, 293, 406
BstKTI GATC 8 cut(s) 106, 184, 223, 265, 422, 550, 571, 590
BstMBI GATC 8 cut(s) 103, 181, 220, 262, 419, 547, 568, 587
BstMWI GCNNNNNNNGC 3 cut(s) 290, 299, 611
BstNI CCWGG 2 cut(s) 132, 213
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 130, 211
BstSLI GKGCMC 2 cut(s) 295, 445
BsuRI GGCC 2 cut(s) 354, 392
BtgI CCRYGG 1 cut(s) 355
BtsCI GGATG 3 cut(s) 196, 293, 406
BtsIMutI CAGTG 1 cut(s) 80
Cac8I GCNNGC 2 cut(s) 53, 616
Cfr10I RCCGGY 1 cut(s) 646
CseI GACGC 1 cut(s) 144
Csp6I GTAC 1 cut(s) 228
CspAI ACCGGT 1 cut(s) 646
CviAII CATG 3 cut(s) 52, 356, 394
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 3 cut(s) 29, 246, 432
DpnI GATC 8 cut(s) 105, 183, 222, 264, 421, 549, 570, 589
DpnII GATC 8 cut(s) 103, 181, 220, 262, 419, 547, 568, 587
EaeI YGGCCR 1 cut(s) 352
Eco130I CCWWGG 2 cut(s) 8, 355
Eco57I CTGAAG 3 cut(s) 324, 336, 552
EcoNI CCTNNNNNAGG 1 cut(s) 155
EcoRII CCWGG 2 cut(s) 130, 211
EcoT14I CCWWGG 2 cut(s) 8, 355
EcoT22I ATGCAT 1 cut(s) 196
ErhI CCWWGG 2 cut(s) 8, 355
FaeI CATG 3 cut(s) 55, 359, 397
FatI CATG 3 cut(s) 51, 355, 393
FbaI TGATCA 1 cut(s) 568
FokI GGATG 3 cut(s) 203, 300, 413
FspBI CTAG 1 cut(s) 410
GsaI CCCAGC 1 cut(s) 302
GsuI CTGGAG 2 cut(s) 104, 535
HaeIII GGCC 2 cut(s) 354, 392
HapII CCGG 1 cut(s) 647
HgaI GACGC 1 cut(s) 144
Hin1II CATG 3 cut(s) 55, 359, 397
HindIII AAGCTT 2 cut(s) 534, 612
HinfI GANTC 2 cut(s) 242, 598
HpaII CCGG 1 cut(s) 647
HphI GGTGA 2 cut(s) 230, 636
Hpy166II GTNNAC 1 cut(s) 293
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 8 cut(s) 38, 101, 158, 205, 514, 595, 608, 631
Hpy8I GTNNAC 1 cut(s) 293
HpyAV CCTTC 1 cut(s) 274
HpyCH4V TGCA 6 cut(s) 77, 194, 293, 386, 450, 618
HpyF10VI GCNNNNNNNGC 3 cut(s) 290, 299, 611
HpyF3I CTNAG 3 cut(s) 29, 246, 432
Hsp92II CATG 3 cut(s) 55, 359, 397
KpnI GGTACC 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 568
Kzo9I GATC 8 cut(s) 103, 181, 220, 262, 419, 547, 568, 587
LmnI GCTCC 2 cut(s) 66, 638
LweI GCATC 3 cut(s) 78, 181, 650
MaeI CTAG 1 cut(s) 410
MaeIII GTNAC 1 cut(s) 362
MalI GATC 8 cut(s) 105, 183, 222, 264, 421, 549, 570, 589
MboI GATC 8 cut(s) 103, 181, 220, 262, 419, 547, 568, 587
MboII GAAGA 4 cut(s) 317, 425, 473, 592
MhlI GDGCHC 2 cut(s) 295, 445
MluCI AATT 5 cut(s) 268, 308, 380, 465, 524
MlyI GAGTC 1 cut(s) 607
MmeI TCCRAC 1 cut(s) 575
MnlI CCTC 9 cut(s) 34, 79, 154, 161, 268, 347, 439, 463, 632
Mph1103I ATGCAT 1 cut(s) 196
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 2 cut(s) 360, 649
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 1 cut(s) 647
MspR9I CCNGG 2 cut(s) 132, 213
MvaI CCWGG 2 cut(s) 132, 213
MwoI GCNNNNNNNGC 3 cut(s) 290, 299, 611
NcoI CCATGG 1 cut(s) 355
NdeII GATC 8 cut(s) 103, 181, 220, 262, 419, 547, 568, 587
NlaIII CATG 3 cut(s) 55, 359, 397
NlaIV GGNNCC 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 362
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 1 cut(s) 55
PaeI GCATGC 1 cut(s) 55
PfeI GAWTC 1 cut(s) 242
PinAI ACCGGT 1 cut(s) 646
PleI GAGTC 1 cut(s) 606
PpsI GAGTC 1 cut(s) 606
Psp6I CCWGG 2 cut(s) 130, 211
PspFI CCCAGC 1 cut(s) 298
PspGI CCWGG 2 cut(s) 130, 211
PspN4I GGNNCC 1 cut(s) 229
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
RseI CAYNNNNRTG 2 cut(s) 360, 649
SaqAI TTAA 1 cut(s) 375
Sau3AI GATC 8 cut(s) 103, 181, 220, 262, 419, 547, 568, 587
SchI GAGTC 1 cut(s) 607
ScrFI CCNGG 2 cut(s) 132, 213
SduI GDGCHC 2 cut(s) 295, 445
SetI ASST 7 cut(s) 217, 304, 339, 499, 538, 578, 616
SfaNI GCATC 3 cut(s) 78, 181, 650
SmiMI CAYNNNNRTG 2 cut(s) 360, 649
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
SphI GCATGC 1 cut(s) 55
Sse9I AATT 5 cut(s) 268, 308, 380, 465, 524
SspMI CTAG 1 cut(s) 410
StyD4I CCNGG 2 cut(s) 130, 211
StyI CCWWGG 2 cut(s) 8, 355
TaqI TCGA 3 cut(s) 240, 422, 578
TasI AATT 5 cut(s) 268, 308, 380, 465, 524
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 1 cut(s) 87
TseFI GTSAC 1 cut(s) 362
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 2 cut(s) 140, 156
TspRI CASTG 1 cut(s) 87
VneI GTGCAC 1 cut(s) 291
XagI CCTNNNNNAGG 1 cut(s) 155
XapI RAATTY 1 cut(s) 268
XceI RCATGY 1 cut(s) 55
XspI CTAG 1 cut(s) 410
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.