Rmu_sc0017679.1_g000002

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017679.1
OG0000800
Physical Location & Seq
Reverse (-)
4074 .. 18819
14746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017679.1_g000002.1.cds

Sequence Viewer

Length: 5160 bp
atggcctccgccaccgaaacctccgaaactctcctctccaagctcgaatccgccgattccgccgggattcaccgcctcatctcccactacctaaacccattcgccgaccttcacaaaaagaagacctcaaccgaccaccaaaccctacaacgctctctcgccaaaaaattcacccccttcctcaaccgcgccttagccatcctccccaaacggctcgccgatcctttaaagctagatgccaaatttgcccccgaattgttcgatatttaccgcctctgcctcgactccatggacgccgtctcctcccaattttccggcaagccctacaagtttcacatccagaggatccggttggtttatgcattcgaggcctggggccgccacaaggacgccgagtccgaggcgcttagggttttgaagagcttcaaggcgattgaactaggaacgaacccggtgaatttggaccgtagatttgtgccaggtgtcgagcagggaggctgcgataaggagtttggggctttgctcggagaagtggtgtggatgctagccaagtgtgcttcgttaattcaaagcaaggaagccgaggtgtatgagcggctgctttgtatggttgaggagttgggaccttggtttagggtgttagatgaaactgtacatgacaaggtgcacagacagcttgccatttatctcgagaggtgtactctttttttagtccgggagctatcatccttgggagcaaatctcgtatgcaagttctgccgcataacagtgagcgagtatgcaaagtcatccatcaaagatcatatgttcaaggcttcagaggctttgaagctttcctgcaaggcttcttggactcgtgttatacatctctgtgaactgtttgcaggcaaattaaaggtcaccagtgctgatgcttctgaagatactattttagatgtcttgaatgagtcgtgtacaggaagtgcttttctattggatattcttaaccaagttgataatcatgaggtgaaaagagctatggcagagtgccttgaaacttggtctattgcagcaaatttgtttgggaggctgccaggtccaatgtctgtggtgaagcagtgggttaagatggaatgtaaatgctgtaacaatatgcatattgaggatagcggtccaaccttatacagtctgctgtcatcttctaagaaaatatcaaagaagacaactgagataatcttggagcagggacttcttgcttacgagaaaatgtatgctgtgaaccccgagttttgtcagaagatgcaaatgcaaattataaacatactactgcaagatgtgtatgtgacaccagacagttggttacaaaaagcaagaattttagtgagaaagggaaaagcgttaaaactttacgggatcaaaggtctaaaagactgtattcattgtttatcagaagcaatatcttcacttagtaagatgtacgataacaccagtatccatcaaacatctcctcgccatgaattagctgtggcatattgcttacgtgcgctttgtacccaggaggatgaaccacaatcagagaaaatgtttcaagatatccgtgctgcccttgatgcatggttggacatttctactacagacatgtgctctccagatgacaattgctccccagatgacaaatgtttaatggtgtctgaaaatacaatgatgctcctatacaatattattgatttgttgtctatcaaggctctatcatatgcaatggaagctcaccagttacgccttgaactattccaaaagaagtttgcgtactcttttgtacaaaaggctgaaaagtacaatgaaactagggatacctatcagaactttacacttgaagatcataatctggaagttaaacgatcagttgcccgtgaagtttggtcctttgacactagttcatgtaacttggaaaactgttatcttagtccctataatgtacttcaatgttatcttgaaagtactcttcaggtagggattatccatgaaatagttggaaatggagctgaggccgaggtagttttacagtggggaaagactatttcctgctcacaaagcttgccactatttgcagttgcattttgttcagtcttaggaaaactgtatcataagaagcagctttgggatttggcagagaaggaacttcaaagtgccaaccaatatttgaaggctagtgcaaaagatatttcttgcgtaaagtgcagattgatgcagaaagcaactgttaaactgaatcttggtgatttataccaaagcaaattttatagttcaaggagtgcatctttagagaaattgtcttatgctgaaagtttgtacaaatcagcacttaccaagctaaacctttctgagtggcaaaattctgttagtttccctggacaaactgtacttaaagaagttggatattctgctggcagtatttctagtcattttgtggaaaccaaggtggaaccaaaaagcaagatagaagctaaaaagtctaggactaagaatgcccgaaaaccagtggggagggatcaaggttcagttcctgagtataatttgaggtcaactcagtctagatgccaatcttctcagaaccaaagtgtaagaggtactggtgtccaagttggtcatccaaaacatttgaaacgaagcaccgaatgtgattcttctgataatttaagtaacaaggactttcttttggatttgaaaacttgtgaggtttcatatgggtctgacctaacatgcatttgcaacaaattgaggtgttggcattgtcttcccatggaagttatggaatctgggctactgaaaaactttgtagatttgaaatgggagtatgtccgcaggcgtctctctctcaggcttctgacaggattaggaaattgtttggagagtcaaggtcaaattcatgaaacaaatgaatttatcctgcaaagcgtatcgattttagtcagcaggaacccattttgtataactacctcgtcgattccgtcgacttccttgcttgatttaatggcaaaggaaatccctggggatgtgcttagtgttgagcgtgcgcaagtactgtacagcatatgttggctgtctttaaagattcgttccaagaataccagggttattttctctgatctgcctcatattcatctaccaaaactagtgtcttggctgatgttagcttttgtgctctgccgtgaagttcctgtactttttcagaaggtttctagattgcttgctgctatatttttactttctgcctcaagtgagcttttttctttgacatcttcttgcaaaaagctccacgaaaatcattgggcttcttattttcatcaagcttcaattggcacccatctcaattaccaatttttcacgaaagtatcggagaggtgtaaactccaacaccttttaaacggtgaggttaatgcaatggcatctttatttcagggggcacatgttgctggctcatgcttggtatcagaaacacagaatttacatagggtcgctccagagtcaattcaagaccttgaagaatttgtgacagaattcttttcaggccttccatgcaccacaatcatctgtataagtgtgcttggaggcccttatgctagtttgttacaagaattgttgtgttttccttcacgcatgcatgcatggattctggtttcacgcttgaactctaagaatcaacctatcagtatgcttctgccagtggattcagtattagaaggagattcagatgatgttagttatactggaataaaggattggcactgtccatggggttccactgtggttgatgatgtagctccagagtttagattgatattggaggggactcattcgtcatctgtgaaacatcctgtacaagatacaaatgagaaaaagcttaactggtgggtgcagaggaagaattttgatcgccgccttggtaaatttctgaagaacttggaagattcatggtttggtacttggaaacttgtcctactgggtgaatggtcaaactgcacgcagttggacttggtacataagaagctggtgcgcaatctgaaatccaaatgcaaaatggagatagatgagagtcttttgaaagtcatccttgggggttccaaatatgccctcgaaggaggtgcacgtgttacacagctttgtcataagaaaggttcctacgttagtaaagttggatatttagaggaagagaagtgtatggcatcatgcaatgattctaatggtggcgagaacttgtctgagttggcctccaaactagttttagaagcagtgaatgagcttgaaggtttacattcttctttaaatatagagccaaccatattagttgtggactacgaggtgcagatgcttccttgggagaatttaccaatcctaagaaaccaggaggtttatcgcatgccctctgttgggagcatttcggcaacagccaatagaaactaccacaatcaagatcaagttcagagtattgtgacttcatttcctttgatagatcccttggatgccttttatcttttgaatcccagtggtgatctaaattatacacaaaatgaatttgagacatggttcagagatcaaaacttggaggggaaagccggatgtgcacctcctgctgaagaattggcagtagcgttgaaaagccatgatctatttatgtattttggccatggatgtggtttcctaataattcgttttgaactttatttgtgtacatgctgtggtacagggcaacaatatattcctagacgtgagattcagagactggaacactgtgccgcaactctccttatgggatgcagtagtggttctctgaaactgaatggatgttatattccacaaggtactccactatcctatctgctggccggttctcctgtcattgtcgccaatttgtgggatgtgacggacagggatattgaccgatttgcaaagtccatgcttgattcttggttgaaagcaaggtcgagtccctgtgtgagttgtgcccaatgcaacaagaaagtcacaaaaaagaaattggccacaaacctgtcttgtgagcataggccgaaggttggatcattcatgagcgaagcacgtaaaacttgccagttacctttcttgattggagcagcaccagtatgctatggtgtcccaactggcatatggaaaaaggagttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

1719

Amino Acids

193.59

Weight (kDa)

7.86

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1295
AasI GACNNNNNNGTC 1 cut(s) 3835
Acc16I TGCGCA 2 cut(s) 3054, 4034
AccB1I GGYRCC 1 cut(s) 3338
AccB7I CCANNNNNTGG 1 cut(s) 4890
AccBSI CCGCTC 1 cut(s) 595
AccI GTMKAC 1 cut(s) 2990
AccII CGCG 1 cut(s) 189
AclWI GGATC 7 cut(s) 215, 340, 353, 1400, 2535, 4484, 5062
AcoI YGGCCR 3 cut(s) 4660, 4860, 5016
AcuI CTGAAG 5 cut(s) 801, 939, 1967, 3953, 4632
AcvI CACGTG 1 cut(s) 4127
AcyI GRCGYC 3 cut(s) 294, 390, 2845
AfiI CCNNNNNNNGG 5 cut(s) 385, 4406, 4407, 4890, 5051
AflIII ACRYGT 3 cut(s) 1617, 3445, 4126
AhdI GACNNNNNGTC 1 cut(s) 394
AhlI ACTAGT 3 cut(s) 1910, 3151, 4255
AjiI CACGTC 1 cut(s) 4745
AjnI CCWGG 8 cut(s) 371, 478, 1072, 1533, 2386, 3025, 3107, 4380
AjuI GAANNNNNNNTTGG 2 cut(s) 3742, 3774
Alw21I GWGCWC 5 cut(s) 669, 1625, 3183, 4126, 4603
Alw26I GTCTC 4 cut(s) 304, 2852, 4550, 4750
Alw44I GTGCAC 3 cut(s) 665, 4122, 4599
AlwI GGATC 7 cut(s) 215, 340, 353, 1400, 2535, 4484, 5062
AlwNI CAGNNNCTG 2 cut(s) 2543, 4759
Ama87I CYCGRG 2 cut(s) 689, 1262
ApaLI GTGCAC 3 cut(s) 665, 4122, 4599
ApeKI GCWGC 8 cut(s) 498, 598, 1049, 1069, 1580, 2131, 3230, 5108
ArsI GACNNNNNNTTYG 6 cut(s) 455, 487, 882, 914, 3140, 3172
Asp700I GAANNNNTTC 1 cut(s) 422
AspLEI GCGC 5 cut(s) 191, 406, 1525, 3055, 4035
AspS9I GGNCC 7 cut(s) 376, 463, 623, 1076, 1151, 1899, 3590
AsuC2I CCSGG 3 cut(s) 64, 452, 716
AsuNHI GCTAGC 1 cut(s) 544
AvaI CYCGRG 2 cut(s) 689, 1262
AvaII GGWCC 5 cut(s) 463, 623, 1076, 1151, 1899
BaeGI GKGCMC 5 cut(s) 669, 3445, 4126, 4603, 4984
BaeI ACNNNNGTAYC 6 cut(s) 1453, 1486, 2598, 2631, 3354, 3387
BalI TGGCCA 2 cut(s) 4662, 5018
BamHI GGATCC 1 cut(s) 345
BanI GGYRCC 1 cut(s) 3338
BarI GAAGNNNNNNTAC 6 cut(s) 1769, 1801, 3183, 3215, 4456, 4488
BauI CACGAG 1 cut(s) 855
BbrPI CACGTG 1 cut(s) 4127
BbsI GAAGAC 3 cut(s) 128, 1205, 2765
Bbv12I GWGCWC 5 cut(s) 669, 1625, 3183, 4126, 4603
BbvCI CCTCAGC 1 cut(s) 2022
BbvI GCAGC 8 cut(s) 485, 585, 1056, 1061, 1567, 2143, 3217, 5120
BccI CCATC 5 cut(s) 206, 800, 1102, 1482, 3351
BceAI ACGGC 3 cut(s) 227, 281, 3171
BcgI CGANNNNNNTGC 4 cut(s) 2980, 3014, 4103, 4137
BciT130I CCWGG 8 cut(s) 373, 480, 1074, 1535, 2388, 3027, 3109, 4382
BciVI GTATCC 2 cut(s) 1481, 1822
BcnI CCSGG 3 cut(s) 64, 452, 716
BcoDI GTCTC 4 cut(s) 304, 2852, 4550, 4750
BcuI ACTAGT 3 cut(s) 1910, 3151, 4255
BfmI CTRYAG 1 cut(s) 1611
BfoI RGCGCY 1 cut(s) 407
BfuI GTATCC 2 cut(s) 1481, 1822
BmcAI AGTACT 2 cut(s) 1978, 3060
Bme18I GGWCC 5 cut(s) 463, 623, 1076, 1151, 1899
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 2 cut(s) 689, 1262
BmgBI CACGTC 1 cut(s) 4745
BmgT120I GGNCC 7 cut(s) 376, 463, 623, 1076, 1151, 1899, 3590
BmiI GGNNCC 9 cut(s) 347, 377, 624, 2463, 2957, 3340, 3778, 4099, 4156
BmrI ACTGGG 2 cut(s) 3989, 4515
BmtI GCTAGC 1 cut(s) 548
BmuI ACTGGG 2 cut(s) 3989, 4515
BpiI GAAGAC 3 cut(s) 128, 1205, 2765
BplI GAGNNNNNCTC 8 cut(s) 685, 717, 726, 758, 2548, 2580, 4232, 4264
BpmI CTGGAG 3 cut(s) 1611, 3483, 3786
Bpu10I CCTNAGC 3 cut(s) 193, 407, 2022
BpuEI CTTGAG 1 cut(s) 3238
BpuMI CCSGG 3 cut(s) 64, 452, 716
Bsa29I ATCGAT 1 cut(s) 2939
BsaAI YACGTR 3 cut(s) 1520, 4127, 5075
BsaBI GATNNNNATC 3 cut(s) 1833, 1860, 2578
BsaHI GRCGYC 3 cut(s) 294, 390, 2845
BsaWI WCCGGW 1 cut(s) 348
BsaXI ACNNNNNCTCC 6 cut(s) 284, 314, 519, 549, 1625, 1655
Bsc4I CCNNNNNNNGG 5 cut(s) 385, 4406, 4407, 4890, 5051
Bse118I RCCGGY 1 cut(s) 4862
Bse3DI GCAATG 3 cut(s) 1743, 3426, 4216
Bse8I GATNNNNATC 3 cut(s) 1833, 1860, 2578
BseBI CCWGG 8 cut(s) 373, 480, 1074, 1535, 2388, 3027, 3109, 4382
BseCI ATCGAT 1 cut(s) 2939
BseJI GATNNNNATC 3 cut(s) 1833, 1860, 2578
BseLI CCNNNNNNNGG 5 cut(s) 385, 4406, 4407, 4890, 5051
BseMI GCAATG 3 cut(s) 1743, 3426, 4216
BseMII CTCAG 8 cut(s) 1197, 2013, 2352, 2535, 2579, 2600, 2869, 4230
BseRI GAGGAG 4 cut(s) 23, 292, 629, 1476
BseSI GKGCMC 5 cut(s) 669, 3445, 4126, 4603, 4984
BseXI GCAGC 8 cut(s) 485, 585, 1056, 1061, 1567, 2143, 3217, 5120
BsgI GTGCAG 4 cut(s) 2236, 3914, 3982, 4361
Bsh1236I CGCG 1 cut(s) 189
BshNI GGYRCC 1 cut(s) 3338
BshVI ATCGAT 1 cut(s) 2939
BsiHKAI GWGCWC 5 cut(s) 669, 1625, 3183, 4126, 4603
BsiHKCI CYCGRG 2 cut(s) 689, 1262
BsiSI CCGG 7 cut(s) 63, 315, 349, 452, 715, 4593, 4863
BslFI GGGAC 6 cut(s) 636, 1239, 1929, 3841, 4950, 5114
BslI CCNNNNNNNGG 5 cut(s) 385, 4406, 4407, 4890, 5051
BsmAI GTCTC 4 cut(s) 304, 2852, 4550, 4750
BsmBI CGTCTC 2 cut(s) 304, 2852
BsmFI GGGAC 6 cut(s) 636, 1239, 1929, 3841, 4950, 5114
BsmI GAATGC 2 cut(s) 362, 2509
BsoBI CYCGRG 2 cut(s) 689, 1262
Bsp1286I GDGCHC 7 cut(s) 669, 1625, 3183, 3445, 4126, 4603, 4984
Bsp1407I TGTACA 7 cut(s) 652, 953, 1795, 2328, 3063, 3856, 4706
Bsp19I CCATGG 4 cut(s) 288, 2778, 3770, 4663
BspCNI CTCAG 8 cut(s) 1198, 2014, 2353, 2536, 2578, 2599, 2868, 4231
BspDI ATCGAT 1 cut(s) 2939
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 3 cut(s) 1000, 2905, 5061
BspLI GGNNCC 9 cut(s) 347, 377, 624, 2463, 2957, 3340, 3778, 4099, 4156
BspOI GCTAGC 1 cut(s) 548
BspPI GGATC 7 cut(s) 215, 340, 353, 1400, 2535, 4484, 5062
BspQI GCTCTTC 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 3338
BsrBI CCGCTC 1 cut(s) 595
BsrDI GCAATG 3 cut(s) 1743, 3426, 4216
BsrFI RCCGGY 1 cut(s) 4862
BsrGI TGTACA 7 cut(s) 652, 953, 1795, 2328, 3063, 3856, 4706
BssAI RCCGGY 1 cut(s) 4862
BssNI GRCGYC 3 cut(s) 294, 390, 2845
BssSI CACGAG 1 cut(s) 855
Bst2BI CACGAG 1 cut(s) 855
Bst2UI CCWGG 8 cut(s) 373, 480, 1074, 1535, 2388, 3027, 3109, 4382
Bst6I CTCTTC 3 cut(s) 413, 1986, 4182
BstACI GRCGYC 3 cut(s) 294, 390, 2845
BstAPI GCANNNNNTGC 3 cut(s) 756, 3449, 4607
BstAUI TGTACA 7 cut(s) 652, 953, 1795, 2328, 3063, 3856, 4706
BstBAI YACGTR 3 cut(s) 1520, 4127, 5075
BstDSI CCRYGG 4 cut(s) 288, 2778, 3770, 4663
BstEII GGTNACC 1 cut(s) 898
BstFNI CGCG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 5 cut(s) 191, 406, 1525, 3055, 4035
BstMAI GTCTC 4 cut(s) 304, 2852, 4550, 4750
BstNI CCWGG 8 cut(s) 373, 480, 1074, 1535, 2388, 3027, 3109, 4382
BstNSI RCATGY 7 cut(s) 1621, 2742, 3449, 3640, 3644, 4399, 4713
BstPI GGTNACC 1 cut(s) 898
BstSFI CTRYAG 1 cut(s) 1611
BstSLI GKGCMC 5 cut(s) 669, 3445, 4126, 4603, 4984
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 8 cut(s) 485, 585, 1056, 1061, 1567, 2143, 3217, 5120
BstV2I GAAGAC 3 cut(s) 128, 1205, 2765
BstX2I RGATCY 2 cut(s) 345, 4489
BstXI CCANNNNNNTGG 2 cut(s) 1335, 4670
BstYI RGATCY 2 cut(s) 345, 4489
Bsu15I ATCGAT 1 cut(s) 2939
BsuI GTATCC 2 cut(s) 1481, 1822
BsuTUI ATCGAT 1 cut(s) 2939
BtgI CCRYGG 4 cut(s) 288, 2778, 3770, 4663
BtrI CACGTC 1 cut(s) 4745
BtsI GCAGTG 2 cut(s) 1103, 4275
CaiI CAGNNNCTG 2 cut(s) 2543, 4759
CciI TCATGA 3 cut(s) 1000, 2905, 5061
CfoI GCGC 5 cut(s) 191, 406, 1525, 3055, 4035
Cfr10I RCCGGY 1 cut(s) 4862
Cfr13I GGNCC 7 cut(s) 376, 463, 623, 1076, 1151, 1899, 3590
ClaI ATCGAT 1 cut(s) 2939
CseI GACGC 3 cut(s) 302, 398, 2834
CspCI CAANNNNNGTGG 4 cut(s) 1068, 1103, 3769, 3804
DraI TTTAAA 4 cut(s) 228, 3087, 3402, 4302
DrdI GACNNNNNNGTC 1 cut(s) 3835
DriI GACNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 3835
EaeI YGGCCR 3 cut(s) 4660, 4860, 5016
Eam1104I CTCTTC 3 cut(s) 413, 1986, 4182
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 3 cut(s) 413, 1986, 4182
EciI GGCGGA 2 cut(s) 40, 49
Eco147I AGGCCT 2 cut(s) 371, 3549
Eco32I GATATC 1 cut(s) 1573
Eco47I GGWCC 5 cut(s) 463, 623, 1076, 1151, 1899
Eco57I CTGAAG 5 cut(s) 801, 939, 1967, 3953, 4632
Eco72I CACGTG 1 cut(s) 4127
Eco88I CYCGRG 2 cut(s) 689, 1262
Eco91I GGTNACC 1 cut(s) 898
EcoO109I RGGNCCY 2 cut(s) 623, 3590
EcoO65I GGTNACC 1 cut(s) 898
EcoRI GAATTC 1 cut(s) 3536
EcoRII CCWGG 8 cut(s) 371, 478, 1072, 1533, 2386, 3025, 3107, 4380
EcoRV GATATC 1 cut(s) 1573
EcoT22I ATGCAT 6 cut(s) 364, 1137, 1594, 2744, 3642, 3646
Esp3I CGTCTC 2 cut(s) 304, 2852
FalI AAGNNNNNCTT 2 cut(s) 4074, 4106
FaqI GGGAC 6 cut(s) 636, 1239, 1929, 3841, 4950, 5114
FauNDI CATATG 5 cut(s) 804, 1732, 2722, 3071, 5141
FblI GTMKAC 1 cut(s) 2990
FspI TGCGCA 2 cut(s) 3054, 4034
GlaI GCGC 5 cut(s) 190, 405, 1524, 3054, 4034
GsuI CTGGAG 3 cut(s) 1611, 3483, 3786
HaeII RGCGCY 1 cut(s) 407
HapII CCGG 7 cut(s) 63, 315, 349, 452, 715, 4593, 4863
HgaI GACGC 3 cut(s) 302, 398, 2834
HhaI GCGC 5 cut(s) 191, 406, 1525, 3055, 4035
Hin1I GRCGYC 3 cut(s) 294, 390, 2845
Hin6I GCGC 5 cut(s) 189, 404, 1523, 3053, 4033
HinP1I GCGC 5 cut(s) 189, 404, 1523, 3053, 4033
HincII GTYRAC 2 cut(s) 2562, 2991
HindII GTYRAC 2 cut(s) 2562, 2991
HindIII AAGCTT 4 cut(s) 830, 2071, 3327, 3878
HpaII CCGG 7 cut(s) 63, 315, 349, 452, 715, 4593, 4863
Hpy99I CGWCG 2 cut(s) 2983, 2992
HpyCH4IV ACGT 5 cut(s) 1519, 4126, 4161, 4744, 5074
HpySE526I ACGT 5 cut(s) 1519, 4126, 4161, 4744, 5074
Hsp92I GRCGYC 3 cut(s) 294, 390, 2845
HspAI GCGC 5 cut(s) 189, 404, 1523, 3053, 4033
LguI GCTCTTC 1 cut(s) 413
Lsp1109I GCAGC 8 cut(s) 485, 585, 1056, 1061, 1567, 2143, 3217, 5120
MaeII ACGT 5 cut(s) 1519, 4126, 4161, 4744, 5074
MbiI CCGCTC 1 cut(s) 595
MfeI CAATTG 2 cut(s) 1636, 3333
MflI RGATCY 2 cut(s) 345, 4489
MhlI GDGCHC 7 cut(s) 669, 1625, 3183, 3445, 4126, 4603, 4984
MlsI TGGCCA 2 cut(s) 4662, 5018
MluNI TGGCCA 2 cut(s) 4662, 5018
MlyI GAGTC 9 cut(s) 278, 404, 847, 956, 2899, 3512, 3823, 4081, 4972
MmeI TCCRAC 8 cut(s) 1178, 1578, 1990, 2392, 3415, 3987, 4153, 5032
Mox20I TGGCCA 2 cut(s) 4662, 5018
Mph1103I ATGCAT 6 cut(s) 364, 1137, 1594, 2744, 3642, 3646
MroXI GAANNNNTTC 1 cut(s) 422
MscI TGGCCA 2 cut(s) 4662, 5018
MslI CAYNNNNRTG 4 cut(s) 767, 870, 4668, 5116
Msp20I TGGCCA 2 cut(s) 4662, 5018
MspI CCGG 7 cut(s) 63, 315, 349, 452, 715, 4593, 4863
MunI CAATTG 2 cut(s) 1636, 3333
Mva1269I GAATGC 2 cut(s) 362, 2509
MvaI CCWGG 8 cut(s) 373, 480, 1074, 1535, 2388, 3027, 3109, 4382
MvnI CGCG 1 cut(s) 189
NciI CCSGG 3 cut(s) 64, 452, 716
NcoI CCATGG 4 cut(s) 288, 2778, 3770, 4663
NdeI CATATG 5 cut(s) 804, 1732, 2722, 3071, 5141
NheI GCTAGC 1 cut(s) 544
NlaIV GGNNCC 9 cut(s) 347, 377, 624, 2463, 2957, 3340, 3778, 4099, 4156
NmeAIII GCCGAG 3 cut(s) 418, 607, 2053
NmuCI GTSAC 6 cut(s) 898, 1321, 3529, 4468, 4897, 4999
NsbI TGCGCA 2 cut(s) 3054, 4034
NsiI ATGCAT 6 cut(s) 364, 1137, 1594, 2744, 3642, 3646
NspI RCATGY 7 cut(s) 1621, 2742, 3449, 3640, 3644, 4399, 4713
PaeI GCATGC 3 cut(s) 3640, 3644, 4399
PaeR7I CTCGAG 1 cut(s) 689
PagI TCATGA 3 cut(s) 1000, 2905, 5061
PasI CCCWGGG 1 cut(s) 3026
PceI AGGCCT 2 cut(s) 371, 3549
PciI ACATGT 2 cut(s) 1617, 3445
PciSI GCTCTTC 1 cut(s) 413
PcsI WCGNNNNNNNCGW 4 cut(s) 51, 396, 2984, 2987
PctI GAATGC 2 cut(s) 362, 2509
PdmI GAANNNNTTC 1 cut(s) 422
PflFI GACNNNGTC 1 cut(s) 296
PflMI CCANNNNNTGG 1 cut(s) 4890
PfoI TCCNGGA 1 cut(s) 714
PleI GAGTC 9 cut(s) 278, 403, 847, 955, 2898, 3511, 3823, 4080, 4971
PmaCI CACGTG 1 cut(s) 4127
PmlI CACGTG 1 cut(s) 4127
PpsI GAGTC 9 cut(s) 278, 403, 847, 955, 2898, 3511, 3823, 4080, 4971
Ppu21I YACGTR 3 cut(s) 1520, 4127, 5075
PpuMI RGGWCCY 1 cut(s) 623
PscI ACATGT 2 cut(s) 1617, 3445
PsiI TTATAA 1 cut(s) 1295
Psp5II RGGWCCY 1 cut(s) 623
Psp6I CCWGG 8 cut(s) 371, 478, 1072, 1533, 2386, 3025, 3107, 4380
PspCI CACGTG 1 cut(s) 4127
PspEI GGTNACC 1 cut(s) 898
PspGI CCWGG 8 cut(s) 371, 478, 1072, 1533, 2386, 3025, 3107, 4380
PspN4I GGNNCC 9 cut(s) 347, 377, 624, 2463, 2957, 3340, 3778, 4099, 4156
PspPI GGNCC 7 cut(s) 376, 463, 623, 1076, 1151, 1899, 3590
PspPPI RGGWCCY 1 cut(s) 623
PstNI CAGNNNCTG 2 cut(s) 2543, 4759
PsuI RGATCY 2 cut(s) 345, 4489
PsyI GACNNNGTC 1 cut(s) 296
RseI CAYNNNNRTG 4 cut(s) 767, 870, 4668, 5116
SalI GTCGAC 1 cut(s) 2989
SapI GCTCTTC 1 cut(s) 413
Sau96I GGNCC 7 cut(s) 376, 463, 623, 1076, 1151, 1899, 3590
ScaI AGTACT 2 cut(s) 1978, 3060
SchI GAGTC 9 cut(s) 278, 404, 847, 956, 2899, 3512, 3823, 4081, 4972
SduI GDGCHC 7 cut(s) 669, 1625, 3183, 3445, 4126, 4603, 4984
SfcI CTRYAG 1 cut(s) 1611
Sfr274I CTCGAG 1 cut(s) 689
SinI GGWCC 5 cut(s) 463, 623, 1076, 1151, 1899
SlaI CTCGAG 1 cut(s) 689
SmiMI CAYNNNNRTG 4 cut(s) 767, 870, 4668, 5116
SmlI CTYRAG 2 cut(s) 689, 3253
SmoI CTYRAG 2 cut(s) 689, 3253
SpeI ACTAGT 3 cut(s) 1910, 3151, 4255
SphI GCATGC 3 cut(s) 3640, 3644, 4399
SseBI AGGCCT 2 cut(s) 371, 3549
SspI AATATT 2 cut(s) 1699, 2177
StuI AGGCCT 2 cut(s) 371, 3549
TaiI ACGT 5 cut(s) 1522, 4129, 4164, 4747, 5077
TaqII GACCGA 1 cut(s) 4932
TauI GCSGC 5 cut(s) 381, 598, 762, 3918, 4775
TseFI GTSAC 6 cut(s) 898, 1321, 3529, 4468, 4897, 4999
TseI GCWGC 8 cut(s) 498, 598, 1049, 1069, 1580, 2131, 3230, 5108
Tsp45I GTSAC 6 cut(s) 898, 1321, 3529, 4468, 4897, 4999
TspGWI ACGGA 3 cut(s) 1565, 2976, 4916
Tth111I GACNNNGTC 1 cut(s) 296
Van91I CCANNNNNTGG 1 cut(s) 4890
VneI GTGCAC 3 cut(s) 665, 4122, 4599
VpaK11BI GGWCC 5 cut(s) 463, 623, 1076, 1151, 1899
XbaI TCTAGA 2 cut(s) 2570, 3218
XceI RCATGY 7 cut(s) 1621, 2742, 3449, 3640, 3644, 4399, 4713
XcmI CCANNNNNNNNNTGG 5 cut(s) 2006, 2785, 4054, 4324, 5139
XhoI CTCGAG 1 cut(s) 689
XmiI GTMKAC 1 cut(s) 2990
XmnI GAANNNNTTC 1 cut(s) 422
ZrmI AGTACT 2 cut(s) 1978, 3060
Zsp2I ATGCAT 6 cut(s) 364, 1137, 1594, 2744, 3642, 3646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.