Rh7AG362500

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000800
Physical Location & Seq
Reverse (-)
46622646 .. 46623252
607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG362500.1

Sequence Viewer

Length: 345 bp
ATGCAGCAGTGGTTCTCTGAAACTGAATGGATGTTATGTTCCACAAGGTACTCCACTATCCTATCTGCTGGCTGGTTCTCCTGTCATTCTCCTGATATTAACCGATTTGCAAATTCCATGCTTGATTCTTGGTTGAAAGCAAGTTCGAGTTACTGTGAGGGTTGTGCCCAAAGCAACAAGAGAGTCGCAAAGAAGAAATTGGCCACACACCCGTCTTGTGAGCATAGGCCGAAGGTTGGATCATTCATGAGTGAAGCACGTAAAACTTGCCAGTTACCTTTCTTGATTGGAGCAGCACCGGTATGCTATGGTGTCCTAACTGACATATGGAAAAAGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

114

Amino Acids

12.93

Weight (kDa)

9.19

Isoelectric Point (pI)

52.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 247
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 1 cut(s) 112
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 1 cut(s) 236
AgeI ACCGGT 1 cut(s) 298
AgsI TTSAA 1 cut(s) 136
AloI GAACNNNNNNTCC 2 cut(s) 22, 54
AlwI GGATC 1 cut(s) 247
AoxI GGCC 2 cut(s) 201, 227
ApeKI GCWGC 2 cut(s) 4, 293
ApoI RAATTY 1 cut(s) 112
AsiGI ACCGGT 1 cut(s) 298
BaeGI GKGCMC 1 cut(s) 169
BalI TGGCCA 1 cut(s) 203
BbvI GCAGC 2 cut(s) 16, 305
BisI GCNGC 2 cut(s) 5, 294
BlsI GCNGC 2 cut(s) 6, 295
BsaAI YACGTR 1 cut(s) 260
BsaWI WCCGGW 1 cut(s) 298
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse118I RCCGGY 1 cut(s) 298
Bse1I ACTGG 1 cut(s) 271
BseGI GGATG 1 cut(s) 36
BseLI CCNNNNNNNGG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 271
BseSI GKGCMC 1 cut(s) 169
BseXI GCAGC 2 cut(s) 16, 305
BshFI GGCC 2 cut(s) 203, 229
BshTI ACCGGT 1 cut(s) 298
BsiSI CCGG 1 cut(s) 299
BslI CCNNNNNNNGG 1 cut(s) 236
BsnI GGCC 2 cut(s) 203, 229
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 1 cut(s) 239
BspANI GGCC 2 cut(s) 203, 229
BspHI TCATGA 1 cut(s) 246
BspPI GGATC 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 298
BsrI ACTGG 1 cut(s) 271
BssAI RCCGGY 1 cut(s) 298
BssMI GATC 1 cut(s) 239
Bst4CI ACNGT 1 cut(s) 155
BstBAI YACGTR 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 70
BstF5I GGATG 1 cut(s) 36
BstKTI GATC 1 cut(s) 242
BstMBI GATC 1 cut(s) 239
BstSLI GKGCMC 1 cut(s) 169
BstV1I GCAGC 2 cut(s) 16, 305
BsuRI GGCC 2 cut(s) 203, 229
BtsCI GGATG 1 cut(s) 36
BtsI GCAGTG 1 cut(s) 14
BtsIMutI CAGTG 1 cut(s) 14
Cac8I GCNNGC 1 cut(s) 70
CciI TCATGA 1 cut(s) 246
Cfr10I RCCGGY 1 cut(s) 298
Csp6I GTAC 1 cut(s) 49
CspAI ACCGGT 1 cut(s) 298
CviAII CATG 2 cut(s) 118, 247
CviJI RGCY 3 cut(s) 72, 203, 229
CviKI_1 RGCY 3 cut(s) 72, 203, 229
CviQI GTAC 1 cut(s) 49
DpnI GATC 1 cut(s) 241
DpnII GATC 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 201
FaeI CATG 2 cut(s) 121, 250
FaiI YATR 8 cut(s) 37, 119, 225, 248, 304, 309, 326, 328
FatI CATG 2 cut(s) 117, 246
FauNDI CATATG 1 cut(s) 326
Fnu4HI GCNGC 2 cut(s) 5, 294
FokI GGATG 1 cut(s) 43
Fsp4HI GCNGC 2 cut(s) 5, 294
GluI GCNGC 2 cut(s) 5, 294
HaeIII GGCC 2 cut(s) 203, 229
HapII CCGG 1 cut(s) 299
Hin1II CATG 2 cut(s) 121, 250
HinfI GANTC 2 cut(s) 125, 183
HpaII CCGG 1 cut(s) 299
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 3 cut(s) 92, 247, 283
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4IV ACGT 1 cut(s) 259
HpyCH4V TGCA 2 cut(s) 4, 110
HpySE526I ACGT 1 cut(s) 259
Hsp92II CATG 2 cut(s) 121, 250
Kzo9I GATC 1 cut(s) 239
LmnI GCTCC 1 cut(s) 290
LpnPI CCDG 6 cut(s) 54, 58, 94, 105, 284, 312
Lsp1109I GCAGC 2 cut(s) 16, 305
MaeII ACGT 1 cut(s) 259
MaeIII GTNAC 2 cut(s) 149, 273
MalI GATC 1 cut(s) 241
MboI GATC 1 cut(s) 239
MboII GAAGA 1 cut(s) 205
MhlI GDGCHC 1 cut(s) 169
MlsI TGGCCA 1 cut(s) 203
MluCI AATT 2 cut(s) 112, 197
MluNI TGGCCA 1 cut(s) 203
MlyI GAGTC 1 cut(s) 192
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 1 cut(s) 151
Mox20I TGGCCA 1 cut(s) 203
MscI TGGCCA 1 cut(s) 203
MseI TTAA 1 cut(s) 99
MslI CAYNNNNRTG 1 cut(s) 301
Msp20I TGGCCA 1 cut(s) 203
MspI CCGG 1 cut(s) 299
NdeI CATATG 1 cut(s) 326
NdeII GATC 1 cut(s) 239
NlaIII CATG 2 cut(s) 121, 250
PagI TCATGA 1 cut(s) 246
PfeI GAWTC 1 cut(s) 125
PinAI ACCGGT 1 cut(s) 298
PkrI GCNGC 2 cut(s) 6, 295
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
Ppu21I YACGTR 1 cut(s) 260
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 301
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 2 cut(s) 5, 294
Sau3AI GATC 1 cut(s) 239
SchI GAGTC 1 cut(s) 192
SduI GDGCHC 1 cut(s) 169
SetI ASST 4 cut(s) 50, 237, 262, 280
SmiMI CAYNNNNRTG 1 cut(s) 301
Sse9I AATT 2 cut(s) 112, 197
TaaI ACNGT 1 cut(s) 155
TaiI ACGT 1 cut(s) 262
TaqI TCGA 1 cut(s) 146
TasI AATT 2 cut(s) 112, 197
TfiI GAWTC 1 cut(s) 125
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 1 cut(s) 14
TseI GCWGC 2 cut(s) 4, 293
TspDTI ATGAA 1 cut(s) 235
TspRI CASTG 1 cut(s) 14
XapI RAATTY 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.