MD15G1419000.v1.1

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000800
Physical Location & Seq
Forward (+)
52032445 .. 52040763
8319 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1419000.v1.1.491

Sequence Viewer

Length: 2496 bp
ATGAGGTGTTGGCAGTGTCTTCCTGTGGAAGTTATGAAATCTGGGTTAGTGAAAGACTTAGTACATTTAAAATGGGAGTTTGTCCGCCGGCGTTTATTGCTCAGGCTTCTCACAGGATTAGGAAAATGCTTGGACAGTCGTGGTCAAACTCATGAGACACATGAAATTATAGTGCAAACTGTATCAGTCTTGGTCAGCCGAAACCCATTTTGTCCTATTACCTCAACTGTTCCATTGACTTCCTTGCTTGATTTAATGGGAAAGGAGATCCCCGGGGATGTGTTCTGTGTTGAACGGGCAGAAGTACTGTTGAACATAAGTTGGTCGTCTTTGAAGAGTTACTGTTCTAAGGAAACCAGGAGTATGTGCTCTGATCTGCCTCATATTCAGCTACCAAAACTAGTGTCTTGGTTGATGCTAGCTTTTGTTCTCTGCCGCGACGTTCCTGTACTTTTCCAGAAGAGGAATGCTATGGTCGACGGTTTGTTTCCAGATTGCTTGCCGCTATATTTGTGCTTTCTACCTCAAGTGATCTTTTTTTCTTTGTCATCTTCTTCTAAAACTCTCCGCGAAAACCATTGGGCTTCTTATTTCCATCAAGCTTCACTTGGCACTCATCTTAGTTGCCAATTTTTCACAAATATATCTGGGATATGTAATGTTCAGCACCTTGTAAACACTGAGGGTTCACATGTTCCTGGCTCAACTTGTTTGGGATCAGAAAAAAAGAATTTACTCAGGCTTGCACCTGAGTCTATTCAAGAACTTGAAGGATTTGTAACACTGTTTTTTGCCGGCCTTCCTTGCACAACAATTATCTGTATAAGTTTGCTCGGAAGCCCTTATGCTAGTTTCCTACAGGAGCTGTTGTCCTTTCATACTTGTGTTCATGCATGGATTCTTGTATCACGCTTGAACTTGAAGAGTCAACCTATTGTTATGCTTCTACCTGTGGATTCAGTATTAGAAGGAGATTCCTCCGATGATACAAGTTCTGGTTCTGTTAGTGTTTCTGACGGAAAGGTTGGAAAGCGTTGGTGCTGCCCATGGGGTTCCACTGTGGTTGATAGGGTAGCTCCAGAATTTAGAATGATATTGGAGGAGAGTTATTTGTCATCTTCAATCGAAGAAGAAGAAGATACAAAAGAGAATAGGGCGTTATGGTGGATGTGGAGAAATAAGCTTGATCGTCGCCTCTGTAAATTGTTGAAGAACTTAGAAGATTTATGGTTTGGTCCTTGGAAATATTTGCTTCTGGGAGAATCGTCAAACTGCAAGCAGCTGGACTTGGTACATAAGAAGCTGGCGCGGGATTTGAAATCTAAGTGCAAAATGGACATAGATGAGAGTCTTCTGAAAGTCATTCTTGGGGGTTCCAAATATGCCTTTGAAGGTGGAGGCGCATATGTTTCACAGCTTTGTTTCAAGAAAGGTTGCTATATTGGTAAAGCTGGATGTTCCGAGGAAAATAAGTGGTTGGCGTCAACTAATGAATCTAATGGTTATCAGAAACTATCTGAGTTGGCTTTCCAACTAATACAGGGAGCAGTGAATGAGCTTGAAGGGCTAGATACTGTAAATAGAGAGCCCATCATTCTAGTGCTGGACTTCGAGGTGCAGATGCTTCCTTGGGAGAATATACCAATACTAAGAAACCAGGAGGCTTATCGCATGCCTTCTATTGGGAGCATCTTTGCAACGCTGGAGAAGAATTACCATCAAGATAAAGTTGCAAGTAGTACTAAGAAACCAGGGGGCTTATCGCATGCCTTATGTCAGAAGGCTTCATTCCCTTTGATTGATCCATTGGATGCATTTTATCTCCTGAACCCAGGTGGTGATCTAGGTATCACACAAATTGAATTTGAGGAGTGGTTCAGAGATCAAAATTTGGAGGGGAAGGCTGGATGTGCACCCCCAGCTGAAGAATTGGCAGAAGCCTTGAAAAGCCATGACCTCTTTATATATATCGGCCATGGAAGTGGGGTGAACTATATTCCCATGCATCAGATTCAGAGTCTGGAAAATTGTGCTGCTACTCTTTTGATGGGATGTAGTAGTGGTTGTCTGACACTGAATGGTTGTTATGTTCCACACGGTCCCGCACTATCTTATCTGCTGGCTGGTTCTCCTGTCATTATTGGAAATTTATGGGAAGTGACGGACAAGGACATCAACCGATTTGCAAAGGCCATGCTTGATGGTTGGCTAAAAGAAAGATCGAGTTCCTCTGAGGGTTGCGCCCAATGCAAAGTAGCAGAAGAATTTGAGGCATTGAGCATTACGGGCTGTCCAGGTATTGCCAAGAAGAAAGTCTCGAGGAAGAAATTGCCTGAAGCTTGTGAAAGTGATCCAATGACGATTTCTTGTGATCATAGGCCGAAGATTGGATCATTCGCAAGTCAAGCTCGTGAAGCTTGCAGTCTTCCTTTCTTGATTGGAGCATCACCAGTATGTTATGGTGTTCCTACGGGCATAAGGAGAAAAGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

832

Amino Acids

92.39

Weight (kDa)

6.29

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Separin_C PF03568 298 - 725 1.5e-94 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 477
AccII CGCG 3 cut(s) 438, 570, 1309
AciI CCGC 6 cut(s) 85, 436, 503, 568, 1309, 2103
AclWI GGATC 5 cut(s) 262, 724, 1796, 2345, 2398
AcoI YGGCCR 1 cut(s) 1972
AcsI RAATTY 6 cut(s) 730, 1082, 1862, 1888, 2146, 2264
AcuI CTGAAG 2 cut(s) 1944, 2355
AcyI GRCGYC 1 cut(s) 1481
AfaI GTAC 5 cut(s) 63, 306, 450, 1293, 1741
AfiI CCNNNNNNNGG 2 cut(s) 1682, 2387
AflIII ACRYGT 1 cut(s) 691
AhlI ACTAGT 1 cut(s) 400
AjnI CCWGG 6 cut(s) 356, 697, 1656, 1750, 1831, 2293
AjuI GAANNNNNNNTTGG 2 cut(s) 2347, 2379
Alw21I GWGCWC 2 cut(s) 371, 1915
Alw26I GTCTC 2 cut(s) 149, 2320
Alw44I GTGCAC 1 cut(s) 1911
AlwI GGATC 5 cut(s) 262, 724, 1796, 2345, 2398
AlwNI CAGNNNCTG 2 cut(s) 865, 2020
Ama87I CYCGRG 2 cut(s) 272, 2317
AoxI GGCC 4 cut(s) 796, 1972, 2190, 2378
ApaLI GTGCAC 1 cut(s) 1911
ApeKI GCWGC 3 cut(s) 1041, 1279, 2033
ApoI RAATTY 6 cut(s) 730, 1082, 1862, 1888, 2146, 2264
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
AspLEI GCGC 3 cut(s) 1309, 1403, 2243
AspS9I GGNCC 2 cut(s) 1235, 2099
AsuC2I CCSGG 2 cut(s) 273, 274
AsuHPI GGTGA 3 cut(s) 1850, 1999, 2439
AsuNHI GCTAGC 1 cut(s) 418
AvaI CYCGRG 2 cut(s) 272, 2317
AvaII GGWCC 2 cut(s) 1235, 2099
BaeGI GKGCMC 1 cut(s) 1915
BanII GRGCYC 1 cut(s) 1590
BauI CACGAG 1 cut(s) 2409
BbsI GAAGAC 3 cut(s) 11, 1343, 2417
Bbv12I GWGCWC 2 cut(s) 371, 1915
BbvI GCAGC 3 cut(s) 1028, 1291, 2020
BccI CCATC 5 cut(s) 603, 1598, 1725, 2041, 2195
BciT130I CCWGG 6 cut(s) 358, 699, 1658, 1752, 1833, 2295
BclI TGATCA 1 cut(s) 2371
BcnI CCSGG 2 cut(s) 273, 274
BcoDI GTCTC 2 cut(s) 149, 2320
BcuI ACTAGT 1 cut(s) 400
BfaI CTAG 6 cut(s) 401, 419, 849, 1568, 1598, 1844
BfmI CTRYAG 1 cut(s) 857
BisI GCNGC 5 cut(s) 436, 503, 1042, 1280, 2034
BlsI GCNGC 5 cut(s) 437, 504, 1043, 1281, 2035
BmcAI AGTACT 2 cut(s) 306, 1741
Bme1390I CCNGG 8 cut(s) 273, 274, 358, 699, 1658, 1752, 1833, 2295
Bme18I GGWCC 2 cut(s) 1235, 2099
BmeT110I CYCGRG 2 cut(s) 272, 2317
BmgT120I GGNCC 2 cut(s) 1235, 2099
BmiI GGNNCC 3 cut(s) 1054, 1375, 2101
BmrFI CCNGG 8 cut(s) 273, 274, 358, 699, 1658, 1752, 1833, 2295
BmsI GCATC 6 cut(s) 405, 1611, 1698, 1801, 2014, 2453
BmtI GCTAGC 1 cut(s) 422
BpiI GAAGAC 3 cut(s) 11, 1343, 2417
BpmI CTGGAG 2 cut(s) 1062, 1724
Bpu10I CCTNAGC 1 cut(s) 101
BpuEI CTTGAG 1 cut(s) 510
BpuMI CCSGG 2 cut(s) 273, 274
BsaHI GRCGYC 1 cut(s) 1481
BsaXI ACNNNNNCTCC 2 cut(s) 854, 884
Bsc4I CCNNNNNNNGG 2 cut(s) 1682, 2387
Bse118I RCCGGY 2 cut(s) 87, 794
Bse1I ACTGG 1 cut(s) 2450
BseBI CCWGG 6 cut(s) 358, 699, 1658, 1752, 1833, 2295
BseGI GGATG 6 cut(s) 283, 1173, 1460, 1816, 1913, 2057
BseLI CCNNNNNNNGG 2 cut(s) 1682, 2387
BseMII CTCAG 6 cut(s) 115, 672, 741, 751, 1509, 2223
BseNI ACTGG 1 cut(s) 2450
BseRI GAGGAG 2 cut(s) 1115, 1883
BseSI GKGCMC 1 cut(s) 1915
BseXI GCAGC 3 cut(s) 1028, 1291, 2020
BseYI CCCAGC 1 cut(s) 1918
BsgI GTGCAG 1 cut(s) 1637
Bsh1236I CGCG 3 cut(s) 438, 570, 1309
BshFI GGCC 4 cut(s) 798, 1974, 2192, 2380
BsiHKAI GWGCWC 2 cut(s) 371, 1915
BsiHKCI CYCGRG 2 cut(s) 272, 2317
BsiSI CCGG 3 cut(s) 88, 273, 795
BslFI GGGAC 1 cut(s) 2085
BslI CCNNNNNNNGG 2 cut(s) 1682, 2387
BsmAI GTCTC 2 cut(s) 149, 2320
BsmFI GGGAC 1 cut(s) 2085
BsmI GAATGC 1 cut(s) 472
BsnI GGCC 4 cut(s) 798, 1974, 2192, 2380
BsoBI CYCGRG 2 cut(s) 272, 2317
Bsp1286I GDGCHC 3 cut(s) 371, 1590, 1915
Bsp19I CCATGG 2 cut(s) 1046, 1975
BspACI CCGC 6 cut(s) 85, 436, 503, 568, 1309, 2103
BspANI GGCC 4 cut(s) 798, 1974, 2192, 2380
BspCNI CTCAG 6 cut(s) 114, 673, 742, 750, 1510, 2224
BspFNI CGCG 3 cut(s) 438, 570, 1309
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 3 cut(s) 1054, 1375, 2101
BspOI GCTAGC 1 cut(s) 422
BspPI GGATC 5 cut(s) 262, 724, 1796, 2345, 2398
BsrFI RCCGGY 2 cut(s) 87, 794
BsrI ACTGG 1 cut(s) 2450
BssAI RCCGGY 2 cut(s) 87, 794
BssNI GRCGYC 1 cut(s) 1481
BssSI CACGAG 1 cut(s) 2409
BssT1I CCWWGG 4 cut(s) 1046, 1238, 1628, 1975
Bst2BI CACGAG 1 cut(s) 2409
Bst2UI CCWGG 6 cut(s) 358, 699, 1658, 1752, 1833, 2295
Bst6I CTCTTC 3 cut(s) 329, 455, 917
BstACI GRCGYC 1 cut(s) 1481
BstDSI CCRYGG 2 cut(s) 1046, 1975
BstF5I GGATG 6 cut(s) 283, 1173, 1460, 1816, 1913, 2057
BstFNI CGCG 3 cut(s) 438, 570, 1309
BstHHI GCGC 3 cut(s) 1309, 1403, 2243
BstMAI GTCTC 2 cut(s) 149, 2320
BstMWI GCNNNNNNNGC 9 cut(s) 97, 804, 1564, 1910, 1919, 2247, 2286, 2405, 2414
BstNI CCWGG 6 cut(s) 358, 699, 1658, 1752, 1833, 2295
BstNSI RCATGY 3 cut(s) 695, 1675, 1769
BstSCI CCNGG 8 cut(s) 271, 272, 356, 697, 1656, 1750, 1831, 2293
BstSFI CTRYAG 1 cut(s) 857
BstSLI GKGCMC 1 cut(s) 1915
BstUI CGCG 3 cut(s) 438, 570, 1309
BstV1I GCAGC 3 cut(s) 1028, 1291, 2020
BstV2I GAAGAC 3 cut(s) 11, 1343, 2417
BstX2I RGATCY 1 cut(s) 267
BstXI CCANNNNNNTGG 1 cut(s) 1982
BstYI RGATCY 1 cut(s) 267
BsuRI GGCC 4 cut(s) 798, 1974, 2192, 2380
BtgI CCRYGG 2 cut(s) 1046, 1975
BtsCI GGATG 6 cut(s) 283, 1173, 1460, 1816, 1913, 2057
BtsI GCAGTG 2 cut(s) 20, 1554
BtsIMutI CAGTG 6 cut(s) 20, 678, 782, 1056, 1554, 2072
CaiI CAGNNNCTG 2 cut(s) 865, 2020
CciI TCATGA 1 cut(s) 151
CfoI GCGC 3 cut(s) 1309, 1403, 2243
Cfr10I RCCGGY 2 cut(s) 87, 794
Cfr13I GGNCC 2 cut(s) 1235, 2099
Cfr9I CCCGGG 1 cut(s) 272
CseI GACGC 1 cut(s) 1470
Csp6I GTAC 5 cut(s) 62, 305, 449, 1292, 1740
CspCI CAANNNNNGTGG 2 cut(s) 1045, 1080
CviQI GTAC 5 cut(s) 62, 305, 449, 1292, 1740
DraI TTTAAA 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 1972
Eam1104I CTCTTC 3 cut(s) 329, 455, 917
EarI CTCTTC 3 cut(s) 329, 455, 917
EciI GGCGGA 1 cut(s) 74
Eco130I CCWWGG 4 cut(s) 1046, 1238, 1628, 1975
Eco24I GRGCYC 1 cut(s) 1590
Eco47I GGWCC 2 cut(s) 1235, 2099
Eco57I CTGAAG 2 cut(s) 1944, 2355
Eco88I CYCGRG 2 cut(s) 272, 2317
EcoRII CCWGG 6 cut(s) 356, 697, 1656, 1750, 1831, 2293
EcoT14I CCWWGG 4 cut(s) 1046, 1238, 1628, 1975
EcoT22I ATGCAT 3 cut(s) 895, 1816, 2007
EcoT38I GRGCYC 1 cut(s) 1590
ErhI CCWWGG 4 cut(s) 1046, 1238, 1628, 1975
FalI AAGNNNNNCTT 4 cut(s) 591, 623, 1350, 1382
FaqI GGGAC 1 cut(s) 2085
FauI CCCGC 2 cut(s) 1302, 2110
FauNDI CATATG 1 cut(s) 1405
FbaI TGATCA 1 cut(s) 2371
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 5 cut(s) 436, 503, 1042, 1280, 2034
FokI GGATG 6 cut(s) 290, 1180, 1467, 1823, 1920, 2064
FriOI GRGCYC 1 cut(s) 1590
Fsp4HI GCNGC 5 cut(s) 436, 503, 1042, 1280, 2034
FspBI CTAG 6 cut(s) 401, 419, 849, 1568, 1598, 1844
GlaI GCGC 3 cut(s) 1308, 1402, 2242
GluI GCNGC 5 cut(s) 436, 503, 1042, 1280, 2034
GsaI CCCAGC 1 cut(s) 1922
GsuI CTGGAG 2 cut(s) 1062, 1724
HaeIII GGCC 4 cut(s) 798, 1974, 2192, 2380
HapII CCGG 3 cut(s) 88, 273, 795
HgaI GACGC 1 cut(s) 1470
HhaI GCGC 3 cut(s) 1309, 1403, 2243
Hin1I GRCGYC 1 cut(s) 1481
Hin6I GCGC 3 cut(s) 1307, 1401, 2241
HinP1I GCGC 3 cut(s) 1307, 1401, 2241
HincII GTYRAC 3 cut(s) 478, 929, 1485
HindII GTYRAC 3 cut(s) 478, 929, 1485
HindIII AAGCTT 4 cut(s) 600, 1181, 2337, 2415
HpaII CCGG 3 cut(s) 88, 273, 795
HphI GGTGA 3 cut(s) 1850, 1999, 2439
Hpy166II GTNNAC 7 cut(s) 478, 676, 689, 929, 1485, 1913, 1990
Hpy8I GTNNAC 7 cut(s) 478, 676, 689, 929, 1485, 1913, 1990
Hpy99I CGWCG 3 cut(s) 443, 482, 1194
HpyAV CCTTC 8 cut(s) 764, 809, 962, 1385, 1556, 1686, 1774, 1894
HpyCH4IV ACGT 1 cut(s) 441
HpyF10VI GCNNNNNNNGC 9 cut(s) 97, 804, 1564, 1910, 1919, 2247, 2286, 2405, 2414
HpySE526I ACGT 1 cut(s) 441
Hsp92I GRCGYC 1 cut(s) 1481
HspAI GCGC 3 cut(s) 1307, 1401, 2241
KroI GCCGGC 2 cut(s) 87, 794
KroNI GCCGGC 2 cut(s) 89, 796
Ksp22I TGATCA 1 cut(s) 2371
LmnI GCTCC 5 cut(s) 862, 1081, 1544, 1686, 2441
Lsp1109I GCAGC 3 cut(s) 1028, 1291, 2020
LweI GCATC 6 cut(s) 405, 1611, 1698, 1801, 2014, 2453
MaeI CTAG 6 cut(s) 401, 419, 849, 1568, 1598, 1844
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 3 cut(s) 338, 778, 2158
MflI RGATCY 1 cut(s) 267
MhlI GDGCHC 3 cut(s) 371, 1590, 1915
MlyI GAGTC 4 cut(s) 761, 934, 1357, 2026
MmeI TCCRAC 2 cut(s) 1006, 1555
Mph1103I ATGCAT 3 cut(s) 895, 1816, 2007
MreI CGCCGGCG 1 cut(s) 87
MroNI GCCGGC 2 cut(s) 87, 794
MseI TTAA 2 cut(s) 68, 254
MslI CAYNNNNRTG 4 cut(s) 882, 1598, 1980, 2452
MspA1I CMGCKG 2 cut(s) 1282, 1922
MspI CCGG 3 cut(s) 88, 273, 795
MspR9I CCNGG 8 cut(s) 273, 274, 358, 699, 1658, 1752, 1833, 2295
Mva1269I GAATGC 1 cut(s) 472
MvaI CCWGG 6 cut(s) 358, 699, 1658, 1752, 1833, 2295
MvnI CGCG 3 cut(s) 438, 570, 1309
MwoI GCNNNNNNNGC 9 cut(s) 97, 804, 1564, 1910, 1919, 2247, 2286, 2405, 2414
NaeI GCCGGC 2 cut(s) 89, 796
NciI CCSGG 2 cut(s) 273, 274
NcoI CCATGG 2 cut(s) 1046, 1975
NdeI CATATG 1 cut(s) 1405
NgoMIV GCCGGC 2 cut(s) 87, 794
NheI GCTAGC 1 cut(s) 418
NlaIV GGNNCC 3 cut(s) 1054, 1375, 2101
NmuCI GTSAC 1 cut(s) 2158
NsiI ATGCAT 3 cut(s) 895, 1816, 2007
NspI RCATGY 3 cut(s) 695, 1675, 1769
PaeI GCATGC 2 cut(s) 1675, 1769
PaeR7I CTCGAG 1 cut(s) 2317
PagI TCATGA 1 cut(s) 151
PciI ACATGT 1 cut(s) 691
PctI GAATGC 1 cut(s) 472
PdiI GCCGGC 2 cut(s) 89, 796
PfeI GAWTC 6 cut(s) 898, 956, 974, 1262, 1493, 2011
PkrI GCNGC 5 cut(s) 437, 504, 1043, 1281, 2035
PleI GAGTC 4 cut(s) 760, 933, 1356, 2025
PpsI GAGTC 4 cut(s) 760, 933, 1356, 2025
PscI ACATGT 1 cut(s) 691
Psp6I CCWGG 6 cut(s) 356, 697, 1656, 1750, 1831, 2293
PspFI CCCAGC 1 cut(s) 1918
PspGI CCWGG 6 cut(s) 356, 697, 1656, 1750, 1831, 2293
PspN4I GGNNCC 3 cut(s) 1054, 1375, 2101
PspPI GGNCC 2 cut(s) 1235, 2099
PstNI CAGNNNCTG 2 cut(s) 865, 2020
PsuI RGATCY 1 cut(s) 267
PvuII CAGCTG 2 cut(s) 1282, 1922
RsaI GTAC 5 cut(s) 63, 306, 450, 1293, 1741
RsaNI GTAC 5 cut(s) 62, 305, 449, 1292, 1740
RseI CAYNNNNRTG 4 cut(s) 882, 1598, 1980, 2452
SalI GTCGAC 1 cut(s) 476
SaqAI TTAA 2 cut(s) 68, 254
SatI GCNGC 5 cut(s) 436, 503, 1042, 1280, 2034
Sau96I GGNCC 2 cut(s) 1235, 2099
ScaI AGTACT 2 cut(s) 306, 1741
SchI GAGTC 4 cut(s) 761, 934, 1357, 2026
ScrFI CCNGG 8 cut(s) 273, 274, 358, 699, 1658, 1752, 1833, 2295
SduI GDGCHC 3 cut(s) 371, 1590, 1915
SfaNI GCATC 6 cut(s) 405, 1611, 1698, 1801, 2014, 2453
SfcI CTRYAG 1 cut(s) 857
Sfr274I CTCGAG 1 cut(s) 2317
SgrAI CRCCGGYG 1 cut(s) 87
SinI GGWCC 2 cut(s) 1235, 2099
SlaI CTCGAG 1 cut(s) 2317
SmaI CCCGGG 1 cut(s) 274
SmiMI CAYNNNNRTG 4 cut(s) 882, 1598, 1980, 2452
SmlI CTYRAG 2 cut(s) 525, 2317
SmoI CTYRAG 2 cut(s) 525, 2317
SpeI ACTAGT 1 cut(s) 400
SphI GCATGC 2 cut(s) 1675, 1769
SsiI CCGC 6 cut(s) 85, 436, 503, 568, 1309, 2103
SspI AATATT 1 cut(s) 1247
SspMI CTAG 6 cut(s) 401, 419, 849, 1568, 1598, 1844
StyD4I CCNGG 8 cut(s) 271, 272, 356, 697, 1656, 1750, 1831, 2293
StyI CCWWGG 4 cut(s) 1046, 1238, 1628, 1975
TaiI ACGT 1 cut(s) 444
TaqI TCGA 5 cut(s) 477, 1125, 1611, 2222, 2318
TatI WGTACW 4 cut(s) 61, 304, 448, 1739
TauI GCSGC 2 cut(s) 438, 505
TfiI GAWTC 6 cut(s) 898, 956, 974, 1262, 1493, 2011
Tru1I TTAA 2 cut(s) 68, 254
Tru9I TTAA 2 cut(s) 68, 254
TscAI CASTG 6 cut(s) 20, 685, 789, 1063, 1554, 2079
TseFI GTSAC 1 cut(s) 2158
TseI GCWGC 3 cut(s) 1041, 1279, 2033
Tsp45I GTSAC 1 cut(s) 2158
TspDTI ATGAA 6 cut(s) 50, 177, 866, 878, 1506, 1776
TspGWI ACGGA 2 cut(s) 1032, 2177
TspMI CCCGGG 1 cut(s) 272
TspRI CASTG 6 cut(s) 20, 685, 789, 1063, 1554, 2079
VneI GTGCAC 1 cut(s) 1911
VpaK11BI GGWCC 2 cut(s) 1235, 2099
XapI RAATTY 6 cut(s) 730, 1082, 1862, 1888, 2146, 2264
XceI RCATGY 3 cut(s) 695, 1675, 1769
XhoI CTCGAG 1 cut(s) 2317
XmaI CCCGGG 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 6 cut(s) 401, 419, 849, 1568, 1598, 1844
ZrmI AGTACT 2 cut(s) 306, 1741
Zsp2I ATGCAT 3 cut(s) 895, 1816, 2007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.