MD00G1111200.v1.1

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000800
Physical Location & Seq
Forward (+)
23240262 .. 23241703
1442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1111200.v1.1.491

Sequence Viewer

Length: 546 bp
ATGACTCTTGTACAGCACAATCTTTATATGTGCAAACTAGGTAATTTGCTTGCCTTATTCTTATTTCAGAAGAGTGTGCAAATACTTGAAAATGTCATCACCAGTGCCTGGATTCAACCTCCTGGTCTAAAGCATCTATATGTCTCTCTGTACAATACCGGGGTGCATTTGAAAGGGATGCAGCTGTTTGATTGTTCTGTGTATTTCAGTTTGGATGTTGCATCAAAGGCATTGAACTTATATTGTAAGGCATCATGGACTCGTGTTATACGTCTTTGTGAGATGTTTGCACACAAACAGAAGGTGTCCCAGGTTGATCTGTCAGAAGATGCCATTGTAGATTTCTTTAATGAGTGCTGTATGAGAAGTGCTTTTGTTTTGGACGTTCTTAATGAATTGCACAGTTATGATGTGGAAAGAACTTTATTAGAGAGCTGTGAAAATTGGTCTATTGCTGCAAACTTGTTTGGGAGGCTGCCAGGTCCTATGGCTGTGGTGAAGCAGTGGGTTAAGGTATCATCTGAGAGTTTCCTTTACTTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

182

Amino Acids

20.7

Weight (kDa)

6.5

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 108
AfaI GTAC 2 cut(s) 12, 152
AfiI CCNNNNNNNGG 1 cut(s) 108
AgsI TTSAA 4 cut(s) 89, 116, 172, 235
AjnI CCWGG 4 cut(s) 107, 121, 309, 478
AluBI AGCT 2 cut(s) 184, 435
AluI AGCT 2 cut(s) 184, 435
Alw26I GTCTC 1 cut(s) 148
AlwNI CAGNNNCTG 1 cut(s) 108
ApeKI GCWGC 3 cut(s) 181, 455, 475
AspS9I GGNCC 1 cut(s) 482
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 2 cut(s) 91, 508
AvaII GGWCC 1 cut(s) 482
BauI CACGAG 1 cut(s) 261
BbvI GCAGC 3 cut(s) 193, 442, 462
BciT130I CCWGG 4 cut(s) 109, 123, 311, 480
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 148
BfaI CTAG 1 cut(s) 38
BisI GCNGC 3 cut(s) 182, 456, 476
BlsI GCNGC 3 cut(s) 183, 457, 477
Bme1390I CCNGG 5 cut(s) 109, 123, 160, 311, 480
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 1 cut(s) 482
BmrFI CCNGG 5 cut(s) 109, 123, 160, 311, 480
BmsI GCATC 5 cut(s) 142, 168, 230, 260, 319
BpuMI CCSGG 1 cut(s) 160
BsaJI CCNNGG 2 cut(s) 159, 309
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse1I ACTGG 1 cut(s) 102
BseBI CCWGG 4 cut(s) 109, 123, 311, 480
BseDI CCNNGG 2 cut(s) 159, 309
BseGI GGATG 2 cut(s) 183, 220
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 1 cut(s) 102
BseXI GCAGC 3 cut(s) 193, 442, 462
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 1 cut(s) 108
BsmAI GTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 292
Bsp1407I TGTACA 2 cut(s) 10, 150
Bsp143I GATC 1 cut(s) 316
BspCNI CTCAG 1 cut(s) 514
BsrGI TGTACA 2 cut(s) 10, 150
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 2 cut(s) 159, 309
BssMI GATC 1 cut(s) 316
BssSI CACGAG 1 cut(s) 261
Bst2BI CACGAG 1 cut(s) 261
Bst2UI CCWGG 4 cut(s) 109, 123, 311, 480
Bst4CI ACNGT 1 cut(s) 404
Bst6I CTCTTC 1 cut(s) 65
BstAUI TGTACA 2 cut(s) 10, 150
BstC8I GCNNGC 1 cut(s) 51
BstDEI CTNAG 1 cut(s) 522
BstF5I GGATG 2 cut(s) 183, 220
BstKTI GATC 1 cut(s) 319
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 1 cut(s) 316
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 4 cut(s) 109, 123, 311, 480
BstSCI CCNGG 5 cut(s) 107, 121, 158, 309, 478
BstV1I GCAGC 3 cut(s) 193, 442, 462
BtsCI GGATG 2 cut(s) 183, 220
BtsI GCAGTG 1 cut(s) 509
BtsIMutI CAGTG 2 cut(s) 109, 509
Cac8I GCNNGC 1 cut(s) 51
CaiI CAGNNNCTG 1 cut(s) 108
Cfr13I GGNCC 1 cut(s) 482
Csp6I GTAC 2 cut(s) 11, 151
CviAII CATG 1 cut(s) 255
CviJI RGCY 4 cut(s) 184, 435, 475, 491
CviKI_1 RGCY 4 cut(s) 184, 435, 475, 491
CviQI GTAC 2 cut(s) 11, 151
DdeI CTNAG 1 cut(s) 522
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 482
EcoO109I RGGNCCY 1 cut(s) 482
EcoRII CCWGG 4 cut(s) 107, 121, 309, 478
FaeI CATG 1 cut(s) 258
FaqI GGGAC 1 cut(s) 292
FatI CATG 1 cut(s) 254
Fnu4HI GCNGC 3 cut(s) 182, 456, 476
FokI GGATG 2 cut(s) 190, 227
Fsp4HI GCNGC 3 cut(s) 182, 456, 476
FspBI CTAG 1 cut(s) 38
GluI GCNGC 3 cut(s) 182, 456, 476
HapII CCGG 1 cut(s) 159
Hin1II CATG 1 cut(s) 258
HinfI GANTC 3 cut(s) 4, 112, 259
HpaII CCGG 1 cut(s) 159
HphI GGTGA 2 cut(s) 91, 508
Hpy188I TCNGA 3 cut(s) 69, 325, 523
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4IV ACGT 2 cut(s) 271, 384
HpyCH4V TGCA 8 cut(s) 33, 79, 166, 181, 221, 290, 400, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 1 cut(s) 522
HpySE526I ACGT 2 cut(s) 271, 384
Hsp92II CATG 1 cut(s) 258
Kzo9I GATC 1 cut(s) 316
Lsp1109I GCAGC 3 cut(s) 193, 442, 462
LweI GCATC 5 cut(s) 142, 168, 230, 260, 319
MaeI CTAG 1 cut(s) 38
MaeII ACGT 2 cut(s) 271, 384
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 2 cut(s) 82, 338
MluCI AATT 3 cut(s) 43, 395, 442
MlyI GAGTC 1 cut(s) 253
MnlI CCTC 2 cut(s) 129, 465
MseI TTAA 3 cut(s) 348, 390, 510
MslI CAYNNNNRTG 2 cut(s) 138, 405
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 5 cut(s) 109, 123, 160, 311, 480
MvaI CCWGG 4 cut(s) 109, 123, 311, 480
MwoI GCNNNNNNNGC 1 cut(s) 227
NciI CCSGG 1 cut(s) 160
NdeII GATC 1 cut(s) 316
NlaIII CATG 1 cut(s) 258
PcsI WCGNNNNNNNCGW 1 cut(s) 268
PfeI GAWTC 1 cut(s) 112
PflMI CCANNNNNTGG 1 cut(s) 108
PkrI GCNGC 3 cut(s) 183, 457, 477
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PpuMI RGGWCCY 1 cut(s) 482
Psp5II RGGWCCY 1 cut(s) 482
Psp6I CCWGG 4 cut(s) 107, 121, 309, 478
PspGI CCWGG 4 cut(s) 107, 121, 309, 478
PspPI GGNCC 1 cut(s) 482
PspPPI RGGWCCY 1 cut(s) 482
PstNI CAGNNNCTG 1 cut(s) 108
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 2 cut(s) 12, 152
RsaNI GTAC 2 cut(s) 11, 151
RseI CAYNNNNRTG 2 cut(s) 138, 405
SaqAI TTAA 3 cut(s) 348, 390, 510
SatI GCNGC 3 cut(s) 182, 456, 476
Sau3AI GATC 1 cut(s) 316
Sau96I GGNCC 1 cut(s) 482
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 5 cut(s) 109, 123, 160, 311, 480
SfaNI GCATC 5 cut(s) 142, 168, 230, 260, 319
SinI GGWCC 1 cut(s) 482
SmiMI CAYNNNNRTG 2 cut(s) 138, 405
Sse9I AATT 3 cut(s) 43, 395, 442
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 5 cut(s) 107, 121, 158, 309, 478
TaaI ACNGT 1 cut(s) 404
TaiI ACGT 2 cut(s) 274, 387
TasI AATT 3 cut(s) 43, 395, 442
TatI WGTACW 2 cut(s) 10, 150
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 3 cut(s) 348, 390, 510
Tru9I TTAA 3 cut(s) 348, 390, 510
TscAI CASTG 2 cut(s) 109, 509
TseI GCWGC 3 cut(s) 181, 455, 475
TspDTI ATGAA 2 cut(s) 408, 531
TspRI CASTG 2 cut(s) 109, 509
Van91I CCANNNNNTGG 1 cut(s) 108
VpaK11BI GGWCC 1 cut(s) 482
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.