Rw7G029540

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000800
Physical Location & Seq
Forward (+)
41060901 .. 41077451
16551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G029540.1

Sequence Viewer

Length: 6447 bp
ATGGCCTCCGCCACCGAAACCTCCGAAACTCTCCTCTCCAAGCTCGAATCCGCCGATTCCGCCGGGATTCACCGCCTCATCTCCCACTACCTAAACCCATTCGCCGACCTTCACAAAAAGAAGACCTCAACCGACCACCAAACCCTACAACGCTCTCTCGCCAAAAAATTCACCCCCTTCCTCAACCGCGCCTTAGCCATCCTCCCCAAACGGCTCGCCGATCCTTCAAAGCTAGATGCCAAATTTGCCCCCGAATTGTTCGATATTTACCGCCTCTGCCTCGACTCCATGGACGCCGTCTCCTCCCAATTTTCCGGCAAGCCCTACAAGTTTCACATCCAGAGGATCCGGTTGGTTTATGCATTCGAGGCCTGGGGCCGCCACAAGGACGCCGAGTCCGAGGCGCTTAGGGTTTTGAAGAGCTTCAAGGCGATTGAACTAGGAACGAACCCGGTGAATTTGGACCGTAGATTTGTGCCAGGTGTCGAGCAGGGAGGCTGCGATAAGGAGTTTGGGGCTTTGCTCGGAGAAGTGGTGTGGATGCTAGCCAAGTGTGCTTCGTTAATTCAAAGCAACGAAGCCGATGTGTATGAGCGGCTGCTTTGTATGGTTATGGAGTTGGGACCTTGGTTTAGGGTGTTAGATGAAACTGTACATGACAAGGTGCACAGACAGCTTGCCATTTATCTCGAGAGGTGTACTCTTTTTTTAGTCCGGGAGCTATCATCCTTGGGAGCAAATCTTGTATGCAAGTTCTGCCGCATAACAGTGAGCGAGTATGCTAAGTCATCCATCAAAGATCATATGTTCAAGTGTGCCCGTCGAATATGTTCAGCTGTATTTTCATTACAAGAGACTAGTTCCTCATTGATCAGTAACACATTGTTATGCTTGTTTGACTCCCTTGTTGATGAATGCAAGGCTGAGGTCGAATATTCAGGAAAAGATTTTATTGAACTTATTGCTTATTGTGCCAATAAGTGTCGAACTGCAAGTACAAATCTTTCTGGTACTATGAGATCTCATTCAAAAAAATTAATAACTCACTTTGATCAGGATCCACCTTTAAATTTGATTCTAAGACTTTATGCTACTGGTTTGCACTACGTTGATCGTAGTATAAAGTCAAGGGATGAGGATCTTACATCTTCTGGAGGTGCAATTAGAATGTTGCTTGATGATGAGGAGACAATGACTGATTTGTCAGATTGTCTTGCTTCATTAGAAAGTTATTTCCAAATTGGTACAAATGATATTTCCAAAAGTGGGTTTTCCACGAGTCACGTGCAGAAGGATTTAAAGGATTATCTTCTGCCGTATTTTAATGCATTGGAGTTCTTATGCCATCCACTTGCTGACTTAGTATATTCGGGAAAGAAACAGATTTTTGCTGATAATGGAGCTGCCTCTGTTTCTAGTGAACTGTGTGTTATCCAGGGTGCATTTCATCAGTTATATGATTTGTTCCTTTCTTTACAAACGTACAGAATTACTTGTGAGATAGATATAGATGGATTTAAGCACAACAGCATCCTTCGTGCTTCTGTGGCTGCTTTTATACTTTCCACCAGAACGAAGCTTAACATGCAGAAGAGTGCACAAATACTGAACCATGTCATTACCAGCGAATGGATTCAACTTGATGGTCTAAAGCATATATATGTCTCTCTCTACAATACTGGTACATGTTTGTACAAAAACAAGGAGCTGAATGAGGCTTCAGAGGCTTTGAAGCTTTCCTGCAAGGCATCTTGGACTCGTGTTATACATCTCTGTGAACTGTTTGCAGGCAAATTAAAGGCCGCCAGTGCTGATCCTTCTGAAGATACTATTTTAGATGTCTTGAATGAGTCGTGTACAGGAAGTGCTTTTCTATTGGATATTCTTAACCAAGTTGATAATCATGAGGTGAAAAGAGCTATGGCAGAGTGCCTTGAAACTTGGTCTATTGCAGCAAATTTGTTTGGGAGGCTGCCAGGTCCAATGTCTGTGGTGAAGCAGTGGGTTAAGATGGAATGTAAATGCTGTAACAATATGCATATTGAGGATAGCGGTCCAACCTTATACAGTCTGCTGTCATCTTCTAAGAAAATATCAAAGAAGACAACTGAGATAATCTTGGAGCAGGGACTTCTTGCTTATGAGAAAATGTATGCTGTGAACCCTGAGTTTTGTCAGAAGAAGCAAATGCAAATTATAAACATACTACTGCAAGATGTGTATGTGACACCAGACAGTTGGTTACAAAAAGCAAGAATTTTAGTGAGAAAGGGAAGAGCGTTAAAACTTTATGGGATCAAAGGTCTAAAAGACTGTATTCATTGTTTATCAGAAGCAATATCTTCACTTCAGAGTAAGATGTACGATAACACCAGTATCCATCAAACATCTCCTCGCCATGAATTAGCTGTGGCATATTGCTTACGCGCGCTTTGTACCCAGGAGGATGAACCACAATCAGAGAAAATGTTTCAAGATATCCGTGCTGCCCTTGATGCATGGTTGGACATTTCTACTACAGACATGTGCTCTCCAGATGACAATTGCTTCCCAGATGACAAATGTTTAATGGTGTCTGAAAATACAATAATGCTCCTATACAATATTATTGATTTGTTGTCTATCAAGGGTCACATGGATTATTACCATGATATATATAGGCTTTTGATTAGATTATTTAACTGGAAAAATATCCCGTTAGAGAAGTGCTTGGTCAGATTGTGGGAATGCAGGAGAGTTAGTCATGCCCTTTGTACTTCACCTGTTAATGATGCACTCATCATGAGCTTTTCAGATCATTTTGGTGAACTTCCAAAGTCTATTGATTTTTGGATAGATTGTCTTAAAGGATCCCAACAATTGGTAATAGGCTTCCAACATAATTTCTCATTTCTATTTGCAAATTTACCTTGGGGCCCATGCAATCATGCAAGCTTGTTCCGATGTGAAATCACAGTTGATGAAGTTAAAAAAGCTGCTCTTGAACTTATTTCAAGTGTTCCTGTATCAAGTCAGTCGGCTTATGTAGCTGCATATCTCTACTATGATTTATGTGAAAGACTAGTTTCAAATGGACAGTTAGTTGAGGCTCTATCATATGCAATGGAAGCTCACCAGTTACGCCTTGAACTATTCCAAAAGAAGTTTGCGTACTCTTTTGTACGAAAGGCTGAAAAGTACAATGAAACTAGGGATACCTATCAGAACTTTACACTCGAAGATCATAATCTGGAAGTTAAACGATCAGTTGTCCGTGAAGTTTGGTCCTTTGACACTAGTTCATGTAACTTGGAAAACTGTTATCTTAGTCCCTATAATGTACTTCAATGTTATCTTGAAAGTACTCTTCAGGTAGGGATTATCCATGAAATAGTTGGAAATGGAGCTGAGGCCGAGGTAGTTTTACAGTGGGGAAAGACTATTTCCTGCTCACAAAGCTTGCCACTATTTGCAGTTGCATTTTGTTCAGTCTTAGGAAAACTGTATCATAAGAAGCAGCTTTGGGATTTGGCAGAGAAGGAACTTCAAAGTGCCAACCAATATTTGAAGGCTAGTGCAAAAGATATTTCTTGCGTAAAGTGCAGATTGATGCAGAAAGCAACTGTTAAACTGAATCTTGGTGATTTATACCAAAGCAAATTTTATAGTTCAAGGAGTGCATCTTTAGAGAAATTGTCTTATGCTGAAAGTTTGTACAAATCAGCACTTACCAAGCTAAACCTTTCTGAGTGGCAAAATTCTGTTAGTTTCCCTGGACAAACTGTACTTAAAGAAGTTGGATATTCTGCTGGCAGTATTTCTAGTCATTTTGTGGAAACCAAGGTGGAACCAAAAAGCAAGATAGAAGCTAAAAAGTCTAGGACTAAGAATGCCCGAAAACCAGTGGGGAGGGATCAAGTTTCAGTTCCTGAGTATAATTTGAGGTCAACTCAGTCTAGATGCCAATCTTCTCAGAACCAAAGTGTAAGAGGTACTGGTGTCCAAGTTGGTCATCCAAAACATTTGAAACGAAGCACCGAGTGTGATTCTTCTGATAATTTAAGTAACAAGGACTTTCTTTTGGATTTGAAAACTTGTGAGGTTTCATATGGGTCTGACCTAACATGCATTTGCAACAAATTGAGGTGTTGGCATTGTCTTCCCATGGAAGTTATGGAATCTGGGCTACTGAAAAACTTTGTAGATTTGAAATGGGAGTATGTCCGCAGGCGTCTCTCTCTCAGGCTTCTGACAGGATTAGGAAATTGTTTGGAGAGTCAAGGTCAAATTCATGAAACAAATGAATTTATCCTGCAAAGCGTATCGATTTTAGTCAGCAGGAACCCATTTTGTATAACTACCTCATCGATTCCGTCGACTTCCTTGCTTGATTTAATGGCAAAGGAAATCCCTGGGGATGTGCTTAGTGTTGAGCGTGCGGAAGTACTGTACAGCATATGTTGGCTGTCTTTAAAGATTCGTTCCAAGAATACCAGGGTTATTTTCTCTGATCTGCCTCATATTCATCTACCAAAACTAGTGTCTTGGCTGATGTTAGCTTTTGTGCTCTGCCGTGAAGTTCCTGTACTTTTTCAGAAGGTTTCTAGATTGCTTGCTGCTATATTTTTACTTTCTGCCTCAAGTGAGCTTTTTTCTTTGACATCTTCTTGCAAAAAGCTCCACGAAAATCATTGGGCTTCTTATTTTCATCAAGCTTCAATTGGCACCCATCTCAATTACCAATTTTTCACGAAAGTATCGGAGAGGTGTAAACTCCAACACCTTTTAAACGGTGAGGGGGCACATGTTGCTGGCTCATGCTTGGTATCAGAAACACAGAATTTACATAGGGTCGCTCCAGAGTCAATTCAAGACCTTGAAGAATTTGTGACAGAATTCTTTTCAGGCCTTCCATGCACCACAATCATCTGTATAAGTGTGCTTGGAGGCCCTTATACTAGTTTGTTACAAGAATTGTTGTGTTTTCCTTCACGCATGCATGCATGGATTCTGGTTTCACGCTTGAACTCTAAGAATCAACCTATCAGTATGCTTCTGCCAGTGGATTCAGTATTAGAAGGAGATTCAGATGATGTTAGTTATACTGGAATAAAGGATTGGCACTGTCCATGGGGTTCCACTGTGGTTGATGATGTAGCTCCAGAGTTTAGATTGATATTGGAGGGGACTCATTCGTCATCTGTGAAACATCCTGTACAAGATACAAATGAGAAAAAGCTTAACTGGTGGGTGCAGAGGAAGAATTTTGATCGCCGCCTTGGTAAATTTCTGAAGAACTTGGAAGATTCATGGTTTGGTACTTGGAAACTTGTCCTACTGGGTGAATGGTCAAACTGCAAGCAGTTGGACTTGGTACATAAGAAGCTGGTGCGCAATCTGAAATCCAAATGCAAAATGGAGATAGATGAGAGTCTTTTGAAAGTCATCCTTGGGGGTTCCAAATATGCCTTCGAAGGAGGTGCACGTGTTACACAGCTTTGTCTTAAGAAAGGTTCCTACGTTAGTAAAGTTGGATATTTAGAGGAAGAGAAGTGTATGGCATCATGTAATGATTCTAATGGTGGCGAGAACTTGTCTGAGTTGGCCTCCAAACTAGTTTTAGAAGCAGTGAATGAGCTTGAAGGTTTACATTCTTCTTTAAATATAGAGCCAACAATATTAGTTGTGGACTACGAGGTGCAGATGCTTCCTTGGGAGAATTTACCAATCCTAAGAAACCAGGAGGTTTATCGCATGCCCTCTGTTGGGAGCATTTCGGCAACAGCCAATAGAAACTACCACAATCAAGATCAAGTTCAGAGTATTGTGACTTCATTTCCTTTGATAGATCCCTTGGATGCCTTTTATCTTTTGAATCCCAGTGGTGATCTAAATTATACACAAAATGAATTTGAGACATGGTTCAGAGATCAAAACTTGGAGGGGAAAGCCGGATGTGCACCTCCTGCTGAAGAATTGGCAGTAGCGTTGAAAAGCCATGATCTATTTATGTATTTTGGCCATGGATGTGGGCAACAATATATTCCTAGACGTGAGATTCAGAGACTGGAACACTGTGCCGCAACTCTCCTTATGGGATGCAGTAGTGGTTCTCTGAAGCTGAATGGATGTTATGTTCCACAAGGTACTCCACTATCCTATCTGCTGGCCGGTTCTCCTGTCATTGTCGCCAATTTGTGGGATGTGACGGACAGGGATATTGACCGATTTGCAAAGTCCATGCTTGATTCTTGGTTGAAAGCAAGGTCGAGTCCCTGTGTGAGTTGTGCCCAATGCAACAAGAAAGTCACAAAAAAGAAATTGGCCACAAACCTGTCTTGTGAGCATAGGCCGAAGGTTGGATCATTCATGAGCGAAGCACGTAAAACTTGCCAGTTACCTTTCTTGATTGGAGCAGCACCAGTATGCTATGGTGTCCCAACTGGCATATGGAAAAAGGAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

2148

Amino Acids

242.32

Weight (kDa)

7.08

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 9 - 279 6.3e-104 Separase-like, TPR repeats
TPR_ESP1_2nd PF25113 289 - 462 1.8e-39 Separase-like, TPR repeats
Separin_C PF03568 1660 - 2067 4.6e-98 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2198
AasI GACNNNNNNGTC 2 cut(s) 1201, 5173
Acc16I TGCGCA 1 cut(s) 5372
AccB1I GGYRCC 1 cut(s) 4703
AccB7I CCANNNNNTGG 3 cut(s) 1629, 2860, 6177
AccBSI CCGCTC 1 cut(s) 595
AccI GTMKAC 1 cut(s) 4355
AccII CGCG 3 cut(s) 189, 2427, 2429
AcoI YGGCCR 3 cut(s) 5998, 6147, 6303
AcuI CTGAAG 7 cut(s) 1704, 1842, 2333, 3332, 5291, 5970, 6116
AcvI CACGTG 2 cut(s) 1285, 5465
AcyI GRCGYC 3 cut(s) 294, 390, 4210
AdeI CACNNNGTG 1 cut(s) 4020
AfiI CCNNNNNNNGG 8 cut(s) 385, 1266, 1629, 2860, 5744, 5745, 6177, 6338
AflII CTTAAG 1 cut(s) 5483
AflIII ACRYGT 4 cut(s) 1685, 2523, 4783, 5464
AhdI GACNNNNNGTC 1 cut(s) 394
AhlI ACTAGT 6 cut(s) 857, 3061, 3275, 4516, 4937, 5593
AjiI CACGTC 1 cut(s) 6032
AjnI CCWGG 9 cut(s) 371, 478, 1434, 1975, 2439, 3751, 4390, 4472, 5718
AjuI GAANNNNNNNTTGG 4 cut(s) 5080, 5112, 5432, 5464
AloI GAACNNNNNNTCC 2 cut(s) 6099, 6131
Alw21I GWGCWC 6 cut(s) 669, 1600, 2531, 4548, 5464, 5941
Alw26I GTCTC 7 cut(s) 304, 848, 1181, 1669, 4217, 5888, 6037
Alw44I GTGCAC 4 cut(s) 665, 1596, 5460, 5937
AlwNI CAGNNNCTG 2 cut(s) 3908, 6046
Ama87I CYCGRG 1 cut(s) 689
ApaI GGGCCC 1 cut(s) 2918
ApaLI GTGCAC 4 cut(s) 665, 1596, 5460, 5937
ArsI GACNNNNNNTTYG 4 cut(s) 455, 487, 4505, 4537
AseI ATTAAT 1 cut(s) 1037
Asp700I GAANNNNTTC 3 cut(s) 422, 829, 1632
AspLEI GCGC 5 cut(s) 191, 406, 2429, 2431, 5373
AspS9I GGNCC 9 cut(s) 376, 463, 623, 1979, 2054, 2914, 2915, 3264, 4928
AsuC2I CCSGG 3 cut(s) 64, 452, 716
AsuII TTCGAA 1 cut(s) 5451
AsuNHI GCTAGC 1 cut(s) 544
AvaI CYCGRG 1 cut(s) 689
AvaII GGWCC 5 cut(s) 463, 623, 1979, 2054, 3264
BaeGI GKGCMC 8 cut(s) 669, 820, 1600, 2918, 4783, 5464, 5941, 6271
BaeI ACNNNNGTAYC 6 cut(s) 2359, 2392, 3963, 3996, 4719, 4752
BalI TGGCCA 2 cut(s) 6000, 6305
BamHI GGATCC 3 cut(s) 345, 1057, 2849
BanI GGYRCC 1 cut(s) 4703
BanII GRGCYC 1 cut(s) 2918
BarI GAAGNNNNNNTAC 6 cut(s) 3134, 3166, 4548, 4580, 5794, 5826
BauI CACGAG 2 cut(s) 1276, 1758
BbrPI CACGTG 2 cut(s) 1285, 5465
BbsI GAAGAC 3 cut(s) 128, 2108, 4130
Bbv12I GWGCWC 6 cut(s) 669, 1600, 2531, 4548, 5464, 5941
BbvCI CCTCAGC 2 cut(s) 924, 3387
BccI CCATC 8 cut(s) 206, 800, 1353, 1505, 1637, 2005, 2388, 4716
BceAI ACGGC 4 cut(s) 227, 281, 1300, 4536
BcgI CGANNNNNNTGC 6 cut(s) 1267, 1301, 4345, 4379, 5441, 5475
BciT130I CCWGG 9 cut(s) 373, 480, 1436, 1977, 2441, 3753, 4392, 4474, 5720
BciVI GTATCC 2 cut(s) 2387, 3187
BclI TGATCA 2 cut(s) 870, 1051
BcnI CCSGG 3 cut(s) 64, 452, 716
BcoDI GTCTC 7 cut(s) 304, 848, 1181, 1669, 4217, 5888, 6037
BcuI ACTAGT 6 cut(s) 857, 3061, 3275, 4516, 4937, 5593
BfmI CTRYAG 1 cut(s) 2517
BfoI RGCGCY 1 cut(s) 407
BfrI CTTAAG 1 cut(s) 5483
BfuI GTATCC 2 cut(s) 2387, 3187
BglII AGATCT 1 cut(s) 1019
BmcAI AGTACT 2 cut(s) 3343, 4425
Bme18I GGWCC 5 cut(s) 463, 623, 1979, 2054, 3264
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 689
BmgBI CACGTC 1 cut(s) 6032
BmgT120I GGNCC 9 cut(s) 376, 463, 623, 1979, 2054, 2914, 2915, 3264, 4928
BmrI ACTGGG 2 cut(s) 5327, 5853
BmtI GCTAGC 1 cut(s) 548
BmuI ACTGGG 2 cut(s) 5327, 5853
BpiI GAAGAC 3 cut(s) 128, 2108, 4130
BplI GAGNNNNNCTC 6 cut(s) 685, 717, 3913, 3945, 5570, 5602
BpmI CTGGAG 4 cut(s) 1173, 2517, 4821, 5124
Bpu10I CCTNAGC 4 cut(s) 193, 407, 924, 3387
Bpu14I TTCGAA 1 cut(s) 5451
BpuEI CTTGAG 1 cut(s) 4603
BpuMI CCSGG 3 cut(s) 64, 452, 716
Bsa29I ATCGAT 2 cut(s) 4304, 4346
BsaAI YACGTR 3 cut(s) 1285, 5465, 6362
BsaBI GATNNNNATC 5 cut(s) 1056, 1137, 3198, 3225, 3943
BsaHI GRCGYC 3 cut(s) 294, 390, 4210
BsaWI WCCGGW 1 cut(s) 348
BsaXI ACNNNNNCTCC 6 cut(s) 284, 314, 519, 549, 2725, 2755
Bsc4I CCNNNNNNNGG 8 cut(s) 385, 1266, 1629, 2860, 5744, 5745, 6177, 6338
Bse118I RCCGGY 1 cut(s) 6149
Bse3DI GCAATG 1 cut(s) 3108
Bse8I GATNNNNATC 5 cut(s) 1056, 1137, 3198, 3225, 3943
BseBI CCWGG 9 cut(s) 373, 480, 1436, 1977, 2441, 3753, 4392, 4474, 5720
BseCI ATCGAT 2 cut(s) 4304, 4346
BseJI GATNNNNATC 5 cut(s) 1056, 1137, 3198, 3225, 3943
BseLI CCNNNNNNNGG 8 cut(s) 385, 1266, 1629, 2860, 5744, 5745, 6177, 6338
BseMI GCAATG 1 cut(s) 3108
BsePI GCGCGC 1 cut(s) 2427
BseRI GAGGAG 4 cut(s) 23, 292, 1199, 2382
BseSI GKGCMC 8 cut(s) 669, 820, 1600, 2918, 4783, 5464, 5941, 6271
BsgI GTGCAG 4 cut(s) 1307, 3601, 5252, 5699
Bsh1236I CGCG 3 cut(s) 189, 2427, 2429
BshNI GGYRCC 1 cut(s) 4703
BshVI ATCGAT 2 cut(s) 4304, 4346
BsiHKAI GWGCWC 6 cut(s) 669, 1600, 2531, 4548, 5464, 5941
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 7 cut(s) 63, 315, 349, 452, 715, 5931, 6150
BslFI GGGAC 6 cut(s) 636, 2142, 3294, 5179, 6237, 6401
BslI CCNNNNNNNGG 8 cut(s) 385, 1266, 1629, 2860, 5744, 5745, 6177, 6338
BsmAI GTCTC 7 cut(s) 304, 848, 1181, 1669, 4217, 5888, 6037
BsmBI CGTCTC 2 cut(s) 304, 4217
BsmFI GGGAC 6 cut(s) 636, 2142, 3294, 5179, 6237, 6401
BsmI GAATGC 4 cut(s) 362, 920, 2732, 3874
BsoBI CYCGRG 1 cut(s) 689
Bsp119I TTCGAA 1 cut(s) 5451
Bsp120I GGGCCC 1 cut(s) 2914
Bsp1407I TGTACA 6 cut(s) 652, 1692, 1856, 3693, 4428, 5194
Bsp19I CCATGG 4 cut(s) 288, 4143, 5108, 6001
BspDI ATCGAT 2 cut(s) 4304, 4346
BspFNI CGCG 3 cut(s) 189, 2427, 2429
BspHI TCATGA 4 cut(s) 1903, 2781, 4270, 6348
BspOI GCTAGC 1 cut(s) 548
BspQI GCTCTTC 2 cut(s) 413, 2269
BspT104I TTCGAA 1 cut(s) 5451
BspT107I GGYRCC 1 cut(s) 4703
BspTI CTTAAG 1 cut(s) 5483
BsrBI CCGCTC 1 cut(s) 595
BsrDI GCAATG 1 cut(s) 3108
BsrFI RCCGGY 1 cut(s) 6149
BsrGI TGTACA 6 cut(s) 652, 1692, 1856, 3693, 4428, 5194
BssAI RCCGGY 1 cut(s) 6149
BssHII GCGCGC 1 cut(s) 2427
BssNI GRCGYC 3 cut(s) 294, 390, 4210
BssSI CACGAG 2 cut(s) 1276, 1758
Bst2BI CACGAG 2 cut(s) 1276, 1758
Bst2UI CCWGG 9 cut(s) 373, 480, 1436, 1977, 2441, 3753, 4392, 4474, 5720
Bst6I CTCTTC 5 cut(s) 413, 1586, 2269, 3351, 5520
BstACI GRCGYC 3 cut(s) 294, 390, 4210
BstAFI CTTAAG 1 cut(s) 5483
BstAPI GCANNNNNTGC 3 cut(s) 756, 4787, 5945
BstAUI TGTACA 6 cut(s) 652, 1692, 1856, 3693, 4428, 5194
BstBAI YACGTR 3 cut(s) 1285, 5465, 6362
BstBI TTCGAA 1 cut(s) 5451
BstDSI CCRYGG 4 cut(s) 288, 4143, 5108, 6001
BstFNI CGCG 3 cut(s) 189, 2427, 2429
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 5 cut(s) 191, 406, 2429, 2431, 5373
BstMAI GTCTC 7 cut(s) 304, 848, 1181, 1669, 4217, 5888, 6037
BstNI CCWGG 9 cut(s) 373, 480, 1436, 1977, 2441, 3753, 4392, 4474, 5720
BstNSI RCATGY 8 cut(s) 1588, 1689, 2527, 4107, 4787, 4978, 4982, 5737
BstSFI CTRYAG 1 cut(s) 2517
BstSLI GKGCMC 8 cut(s) 669, 820, 1600, 2918, 4783, 5464, 5941, 6271
BstUI CGCG 3 cut(s) 189, 2427, 2429
BstV2I GAAGAC 3 cut(s) 128, 2108, 4130
BstX2I RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2849, 5827
BstXI CCANNNNNNTGG 2 cut(s) 2238, 6008
BstYI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2849, 5827
Bsu15I ATCGAT 2 cut(s) 4304, 4346
BsuI GTATCC 2 cut(s) 2387, 3187
BsuTUI ATCGAT 2 cut(s) 4304, 4346
BtgI CCRYGG 4 cut(s) 288, 4143, 5108, 6001
BtrI CACGTC 1 cut(s) 6032
BtsI GCAGTG 2 cut(s) 2006, 5613
CaiI CAGNNNCTG 2 cut(s) 3908, 6046
CciI TCATGA 4 cut(s) 1903, 2781, 4270, 6348
CfoI GCGC 5 cut(s) 191, 406, 2429, 2431, 5373
Cfr10I RCCGGY 1 cut(s) 6149
Cfr13I GGNCC 9 cut(s) 376, 463, 623, 1979, 2054, 2914, 2915, 3264, 4928
ClaI ATCGAT 2 cut(s) 4304, 4346
CseI GACGC 3 cut(s) 302, 398, 4199
CspCI CAANNNNNGTGG 4 cut(s) 1971, 2006, 5107, 5142
DraI TTTAAA 5 cut(s) 1068, 1299, 4452, 4767, 5640
DraIII CACNNNGTG 1 cut(s) 4020
DrdI GACNNNNNNGTC 2 cut(s) 1201, 5173
DriI GACNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 2 cut(s) 1201, 5173
EaeI YGGCCR 3 cut(s) 5998, 6147, 6303
Eam1104I CTCTTC 5 cut(s) 413, 1586, 2269, 3351, 5520
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 5 cut(s) 413, 1586, 2269, 3351, 5520
EciI GGCGGA 2 cut(s) 40, 49
Eco147I AGGCCT 2 cut(s) 371, 4887
Eco24I GRGCYC 1 cut(s) 2918
Eco32I GATATC 1 cut(s) 2479
Eco47I GGWCC 5 cut(s) 463, 623, 1979, 2054, 3264
Eco57I CTGAAG 7 cut(s) 1704, 1842, 2333, 3332, 5291, 5970, 6116
Eco72I CACGTG 2 cut(s) 1285, 5465
Eco88I CYCGRG 1 cut(s) 689
EcoO109I RGGNCCY 3 cut(s) 623, 2914, 4928
EcoRI GAATTC 1 cut(s) 4874
EcoRII CCWGG 9 cut(s) 371, 478, 1434, 1975, 2439, 3751, 4390, 4472, 5718
EcoRV GATATC 1 cut(s) 2479
EcoT22I ATGCAT 7 cut(s) 364, 1330, 2040, 2500, 4109, 4980, 4984
EcoT38I GRGCYC 1 cut(s) 2918
Esp3I CGTCTC 2 cut(s) 304, 4217
FalI AAGNNNNNCTT 4 cut(s) 2964, 2996, 5412, 5444
FaqI GGGAC 6 cut(s) 636, 2142, 3294, 5179, 6237, 6401
FauNDI CATATG 5 cut(s) 804, 3097, 4087, 4436, 6428
FbaI TGATCA 2 cut(s) 870, 1051
FblI GTMKAC 1 cut(s) 4355
FriOI GRGCYC 1 cut(s) 2918
FspI TGCGCA 1 cut(s) 5372
GlaI GCGC 5 cut(s) 190, 405, 2428, 2430, 5372
GsuI CTGGAG 4 cut(s) 1173, 2517, 4821, 5124
HaeII RGCGCY 1 cut(s) 407
HapII CCGG 7 cut(s) 63, 315, 349, 452, 715, 5931, 6150
HgaI GACGC 3 cut(s) 302, 398, 4199
HhaI GCGC 5 cut(s) 191, 406, 2429, 2431, 5373
Hin1I GRCGYC 3 cut(s) 294, 390, 4210
Hin6I GCGC 5 cut(s) 189, 404, 2427, 2429, 5371
HinP1I GCGC 5 cut(s) 189, 404, 2427, 2429, 5371
HincII GTYRAC 2 cut(s) 3927, 4356
HindII GTYRAC 2 cut(s) 3927, 4356
HindIII AAGCTT 6 cut(s) 1577, 1733, 2932, 3436, 4692, 5216
HpaII CCGG 7 cut(s) 63, 315, 349, 452, 715, 5931, 6150
Hpy99I CGWCG 2 cut(s) 825, 4357
HpyCH4IV ACGT 7 cut(s) 1107, 1284, 1481, 5464, 5499, 6031, 6361
HpySE526I ACGT 7 cut(s) 1107, 1284, 1481, 5464, 5499, 6031, 6361
Hsp92I GRCGYC 3 cut(s) 294, 390, 4210
HspAI GCGC 5 cut(s) 189, 404, 2427, 2429, 5371
Ksp22I TGATCA 2 cut(s) 870, 1051
LguI GCTCTTC 2 cut(s) 413, 2269
MaeII ACGT 7 cut(s) 1107, 1284, 1481, 5464, 5499, 6031, 6361
MbiI CCGCTC 1 cut(s) 595
MfeI CAATTG 3 cut(s) 2542, 2858, 4698
MflI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2849, 5827
MlsI TGGCCA 2 cut(s) 6000, 6305
MluNI TGGCCA 2 cut(s) 6000, 6305
MmeI TCCRAC 9 cut(s) 2081, 2484, 2899, 3355, 3757, 4780, 5325, 5491, 6319
Mox20I TGGCCA 2 cut(s) 6000, 6305
Mph1103I ATGCAT 7 cut(s) 364, 1330, 2040, 2500, 4109, 4980, 4984
MroXI GAANNNNTTC 3 cut(s) 422, 829, 1632
MscI TGGCCA 2 cut(s) 6000, 6305
MslI CAYNNNNRTG 7 cut(s) 767, 886, 1659, 1773, 2802, 6006, 6403
Msp20I TGGCCA 2 cut(s) 6000, 6305
MspA1I CMGCKG 1 cut(s) 836
MspCI CTTAAG 1 cut(s) 5483
MspI CCGG 7 cut(s) 63, 315, 349, 452, 715, 5931, 6150
MunI CAATTG 3 cut(s) 2542, 2858, 4698
Mva1269I GAATGC 4 cut(s) 362, 920, 2732, 3874
MvaI CCWGG 9 cut(s) 373, 480, 1436, 1977, 2441, 3753, 4392, 4474, 5720
MvnI CGCG 3 cut(s) 189, 2427, 2429
NciI CCSGG 3 cut(s) 64, 452, 716
NcoI CCATGG 4 cut(s) 288, 4143, 5108, 6001
NdeI CATATG 5 cut(s) 804, 3097, 4087, 4436, 6428
NheI GCTAGC 1 cut(s) 544
NmeAIII GCCGAG 2 cut(s) 418, 3418
NmuCI GTSAC 7 cut(s) 1280, 2224, 2630, 4867, 5806, 6184, 6286
NsbI TGCGCA 1 cut(s) 5372
NsiI ATGCAT 7 cut(s) 364, 1330, 2040, 2500, 4109, 4980, 4984
NspI RCATGY 8 cut(s) 1588, 1689, 2527, 4107, 4787, 4978, 4982, 5737
NspV TTCGAA 1 cut(s) 5451
PaeI GCATGC 3 cut(s) 4978, 4982, 5737
PaeR7I CTCGAG 1 cut(s) 689
PagI TCATGA 4 cut(s) 1903, 2781, 4270, 6348
PasI CCCWGGG 1 cut(s) 4391
PauI GCGCGC 1 cut(s) 2427
PceI AGGCCT 2 cut(s) 371, 4887
PciI ACATGT 3 cut(s) 1685, 2523, 4783
PciSI GCTCTTC 2 cut(s) 413, 2269
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 396, 4352
PctI GAATGC 4 cut(s) 362, 920, 2732, 3874
PdmI GAANNNNTTC 3 cut(s) 422, 829, 1632
PflFI GACNNNGTC 1 cut(s) 296
PflMI CCANNNNNTGG 3 cut(s) 1629, 2860, 6177
PfoI TCCNGGA 1 cut(s) 714
PmaCI CACGTG 2 cut(s) 1285, 5465
PmlI CACGTG 2 cut(s) 1285, 5465
Ppu21I YACGTR 3 cut(s) 1285, 5465, 6362
PpuMI RGGWCCY 1 cut(s) 623
PscI ACATGT 3 cut(s) 1685, 2523, 4783
PshBI ATTAAT 1 cut(s) 1037
PsiI TTATAA 1 cut(s) 2198
Psp5II RGGWCCY 1 cut(s) 623
Psp6I CCWGG 9 cut(s) 371, 478, 1434, 1975, 2439, 3751, 4390, 4472, 5718
PspCI CACGTG 2 cut(s) 1285, 5465
PspGI CCWGG 9 cut(s) 371, 478, 1434, 1975, 2439, 3751, 4390, 4472, 5718
PspOMI GGGCCC 1 cut(s) 2914
PspPI GGNCC 9 cut(s) 376, 463, 623, 1979, 2054, 2914, 2915, 3264, 4928
PspPPI RGGWCCY 1 cut(s) 623
PsrI GAACNNNNNNTAC 2 cut(s) 979, 1011
PstNI CAGNNNCTG 2 cut(s) 3908, 6046
PsuI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2849, 5827
PsyI GACNNNGTC 1 cut(s) 296
PteI GCGCGC 1 cut(s) 2427
PvuII CAGCTG 1 cut(s) 836
RseI CAYNNNNRTG 7 cut(s) 767, 886, 1659, 1773, 2802, 6006, 6403
SalI GTCGAC 1 cut(s) 4354
SapI GCTCTTC 2 cut(s) 413, 2269
Sau96I GGNCC 9 cut(s) 376, 463, 623, 1979, 2054, 2914, 2915, 3264, 4928
ScaI AGTACT 2 cut(s) 3343, 4425
SfcI CTRYAG 1 cut(s) 2517
Sfr274I CTCGAG 1 cut(s) 689
SfuI TTCGAA 1 cut(s) 5451
SinI GGWCC 5 cut(s) 463, 623, 1979, 2054, 3264
SlaI CTCGAG 1 cut(s) 689
SmiMI CAYNNNNRTG 7 cut(s) 767, 886, 1659, 1773, 2802, 6006, 6403
SmlI CTYRAG 3 cut(s) 689, 4618, 5483
SmoI CTYRAG 3 cut(s) 689, 4618, 5483
SpeI ACTAGT 6 cut(s) 857, 3061, 3275, 4516, 4937, 5593
SphI GCATGC 3 cut(s) 4978, 4982, 5737
SseBI AGGCCT 2 cut(s) 371, 4887
SspI AATATT 4 cut(s) 935, 2605, 3542, 5658
StuI AGGCCT 2 cut(s) 371, 4887
TaiI ACGT 7 cut(s) 1110, 1287, 1484, 5467, 5502, 6034, 6364
TaqII GACCGA 1 cut(s) 6219
TauI GCSGC 6 cut(s) 381, 598, 762, 1805, 5256, 6062
TseFI GTSAC 7 cut(s) 1280, 2224, 2630, 4867, 5806, 6184, 6286
Tsp45I GTSAC 7 cut(s) 1280, 2224, 2630, 4867, 5806, 6184, 6286
TspGWI ACGGA 4 cut(s) 2471, 3242, 4341, 6203
Tth111I GACNNNGTC 1 cut(s) 296
Van91I CCANNNNNTGG 3 cut(s) 1629, 2860, 6177
Vha464I CTTAAG 1 cut(s) 5483
VneI GTGCAC 4 cut(s) 665, 1596, 5460, 5937
VpaK11BI GGWCC 5 cut(s) 463, 623, 1979, 2054, 3264
VspI ATTAAT 1 cut(s) 1037
XbaI TCTAGA 2 cut(s) 3935, 4583
XceI RCATGY 8 cut(s) 1588, 1689, 2527, 4107, 4787, 4978, 4982, 5737
XcmI CCANNNNNNNNNTGG 4 cut(s) 3371, 4150, 5392, 6426
XhoI CTCGAG 1 cut(s) 689
XmiI GTMKAC 1 cut(s) 4355
XmnI GAANNNNTTC 3 cut(s) 422, 829, 1632
ZrmI AGTACT 2 cut(s) 3343, 4425
Zsp2I ATGCAT 7 cut(s) 364, 1330, 2040, 2500, 4109, 4980, 4984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.