Rmu_co8312143.1_g000001

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8312143.1
OG0000800
Physical Location & Seq
Reverse (-)
2 .. 720
719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8312143.1_g000001.1.cds

Sequence Viewer

Length: 459 bp
atggacgccgtctcctcccaattttccggcaagccctacaagtttcacatccagaggatccggttggtttatgcattcgaggcctggggccgccacaaggacgccgagtccgaggcgcttagggttttgaagagcttcaaggcgattgaactaggaacgaacccggtgaatttggaccgtagatttgtgccaggtgtcgagcagggaggctgcgataaggagtttggggctttgctcggagaagtggtgtggatgctagccaagtgtgcttcgttaattcaaagcaaggaagccgaggtgtatgagcggctgctttgtatggttgaggagttgggaccttggtttagggtgttagatgaaactgtacatgacaaggtgcacagacagcttgccatttatctcgagaggtgtactctttttttagtccgggagctatcatccttgggagcaaatctcgta
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

153

Amino Acids

17.46

Weight (kDa)

6.43

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 307
AciI CCGC 2 cut(s) 91, 307
AclWI GGATC 2 cut(s) 52, 65
AcsI RAATTY 1 cut(s) 169
AcyI GRCGYC 2 cut(s) 6, 102
AfaI GTAC 2 cut(s) 366, 412
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 4 cut(s) 130, 139, 149, 281
AhdI GACNNNNNGTC 1 cut(s) 106
AjnI CCWGG 2 cut(s) 83, 190
AluBI AGCT 3 cut(s) 135, 388, 433
AluI AGCT 3 cut(s) 135, 388, 433
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 1 cut(s) 16
Alw44I GTGCAC 1 cut(s) 377
AlwI GGATC 2 cut(s) 52, 65
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 2 cut(s) 81, 88
ApaLI GTGCAC 1 cut(s) 377
ApeKI GCWGC 2 cut(s) 210, 310
ApoI RAATTY 1 cut(s) 169
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
Asp700I GAANNNNTTC 1 cut(s) 134
AspLEI GCGC 1 cut(s) 118
AspS9I GGNCC 3 cut(s) 88, 175, 335
AsuC2I CCSGG 2 cut(s) 164, 428
AsuHPI GGTGA 1 cut(s) 178
AsuNHI GCTAGC 1 cut(s) 256
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 2 cut(s) 175, 335
BaeGI GKGCMC 1 cut(s) 381
BamHI GGATCC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 2 cut(s) 197, 297
BciT130I CCWGG 2 cut(s) 85, 192
BcnI CCSGG 2 cut(s) 164, 428
BcoDI GTCTC 1 cut(s) 16
BfaI CTAG 2 cut(s) 152, 257
BfoI RGCGCY 1 cut(s) 119
BisI GCNGC 4 cut(s) 91, 211, 308, 311
BlsI GCNGC 4 cut(s) 92, 212, 309, 312
Bme1390I CCNGG 4 cut(s) 85, 164, 192, 428
Bme18I GGWCC 2 cut(s) 175, 335
BmeRI GACNNNNNGTC 1 cut(s) 106
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 3 cut(s) 88, 175, 335
BmiI GGNNCC 3 cut(s) 59, 89, 336
BmrFI CCNGG 4 cut(s) 85, 164, 192, 428
BmsI GCATC 1 cut(s) 243
BmtI GCTAGC 1 cut(s) 260
BplI GAGNNNNNCTC 3 cut(s) 397, 429, 438
Bpu10I CCTNAGC 1 cut(s) 119
BpuMI CCSGG 2 cut(s) 164, 428
BsaHI GRCGYC 2 cut(s) 6, 102
BsaJI CCNNGG 5 cut(s) 84, 111, 294, 338, 441
BsaWI WCCGGW 1 cut(s) 60
BsaXI ACNNNNNCTCC 3 cut(s) 26, 231, 261
Bsc4I CCNNNNNNNGG 1 cut(s) 97
BseBI CCWGG 2 cut(s) 85, 192
BseDI CCNNGG 5 cut(s) 84, 111, 294, 338, 441
BseGI GGATG 3 cut(s) 48, 258, 437
BseLI CCNNNNNNNGG 1 cut(s) 97
BseRI GAGGAG 2 cut(s) 4, 341
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 2 cut(s) 197, 297
BshFI GGCC 2 cut(s) 83, 90
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 4 cut(s) 27, 61, 164, 427
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 1 cut(s) 16
BsmBI CGTCTC 1 cut(s) 16
BsmFI GGGAC 1 cut(s) 348
BsmI GAATGC 1 cut(s) 74
BsnI GGCC 2 cut(s) 83, 90
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 1 cut(s) 381
Bsp1407I TGTACA 1 cut(s) 364
Bsp143I GATC 1 cut(s) 57
BspACI CCGC 2 cut(s) 91, 307
BspANI GGCC 2 cut(s) 83, 90
BspLI GGNNCC 3 cut(s) 59, 89, 336
BspOI GCTAGC 1 cut(s) 260
BspPI GGATC 2 cut(s) 52, 65
BspQI GCTCTTC 1 cut(s) 125
BsrBI CCGCTC 1 cut(s) 307
BsrGI TGTACA 1 cut(s) 364
BssECI CCNNGG 5 cut(s) 84, 111, 294, 338, 441
BssMI GATC 1 cut(s) 57
BssNI GRCGYC 2 cut(s) 6, 102
BssT1I CCWWGG 2 cut(s) 338, 441
Bst2UI CCWGG 2 cut(s) 85, 192
Bst4CI ACNGT 2 cut(s) 179, 364
Bst6I CTCTTC 1 cut(s) 125
BstACI GRCGYC 2 cut(s) 6, 102
BstAUI TGTACA 1 cut(s) 364
BstC8I GCNNGC 3 cut(s) 32, 258, 390
BstDEI CTNAG 1 cut(s) 119
BstF5I GGATG 3 cut(s) 48, 258, 437
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 1 cut(s) 60
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 1 cut(s) 57
BstMWI GCNNNNNNNGC 3 cut(s) 80, 266, 385
BstNI CCWGG 2 cut(s) 85, 192
BstSCI CCNGG 4 cut(s) 83, 162, 190, 426
BstSLI GKGCMC 1 cut(s) 381
BstV1I GCAGC 2 cut(s) 197, 297
BstX2I RGATCY 1 cut(s) 57
BstYI RGATCY 1 cut(s) 57
BsuRI GGCC 2 cut(s) 83, 90
BtsCI GGATG 3 cut(s) 48, 258, 437
Cac8I GCNNGC 3 cut(s) 32, 258, 390
CfoI GCGC 1 cut(s) 118
Cfr13I GGNCC 3 cut(s) 88, 175, 335
CseI GACGC 2 cut(s) 14, 110
Csp6I GTAC 2 cut(s) 365, 411
CviAII CATG 1 cut(s) 368
CviQI GTAC 2 cut(s) 365, 411
DdeI CTNAG 1 cut(s) 119
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
DriI GACNNNNNGTC 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 125
Eam1105I GACNNNNNGTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 125
Eco130I CCWWGG 2 cut(s) 338, 441
Eco147I AGGCCT 1 cut(s) 83
Eco47I GGWCC 2 cut(s) 175, 335
Eco88I CYCGRG 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 83, 190
EcoT14I CCWWGG 2 cut(s) 338, 441
EcoT22I ATGCAT 1 cut(s) 76
ErhI CCWWGG 2 cut(s) 338, 441
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 1 cut(s) 371
FaiI YATR 4 cut(s) 72, 303, 320, 369
FaqI GGGAC 1 cut(s) 348
FatI CATG 1 cut(s) 367
Fnu4HI GCNGC 4 cut(s) 91, 211, 308, 311
FokI GGATG 3 cut(s) 35, 265, 424
Fsp4HI GCNGC 4 cut(s) 91, 211, 308, 311
FspBI CTAG 2 cut(s) 152, 257
GlaI GCGC 1 cut(s) 117
GluI GCNGC 4 cut(s) 91, 211, 308, 311
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 2 cut(s) 83, 90
HapII CCGG 4 cut(s) 27, 61, 164, 427
HgaI GACGC 2 cut(s) 14, 110
HhaI GCGC 1 cut(s) 118
Hin1I GRCGYC 2 cut(s) 6, 102
Hin1II CATG 1 cut(s) 371
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HinfI GANTC 1 cut(s) 107
HpaII CCGG 4 cut(s) 27, 61, 164, 427
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 2 cut(s) 379, 411
Hpy188I TCNGA 2 cut(s) 112, 239
Hpy188III TCNNGA 3 cut(s) 52, 401, 403
Hpy8I GTNNAC 2 cut(s) 379, 411
HpyCH4III ACNGT 2 cut(s) 179, 364
HpyCH4V TGCA 2 cut(s) 74, 379
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 266, 385
HpyF3I CTNAG 1 cut(s) 119
Hsp92I GRCGYC 2 cut(s) 6, 102
Hsp92II CATG 1 cut(s) 371
HspAI GCGC 1 cut(s) 116
Kzo9I GATC 1 cut(s) 57
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 2 cut(s) 430, 446
Lsp1109I GCAGC 2 cut(s) 197, 297
LweI GCATC 1 cut(s) 243
MaeI CTAG 2 cut(s) 152, 257
MalI GATC 1 cut(s) 59
MbiI CCGCTC 1 cut(s) 307
MboI GATC 1 cut(s) 57
MboII GAAGA 1 cut(s) 142
MflI RGATCY 1 cut(s) 57
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 3 cut(s) 20, 169, 276
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 8 cut(s) 25, 48, 73, 106, 200, 289, 319, 399
Mph1103I ATGCAT 1 cut(s) 76
MroXI GAANNNNTTC 1 cut(s) 134
MseI TTAA 1 cut(s) 275
MspI CCGG 4 cut(s) 27, 61, 164, 427
MspR9I CCNGG 4 cut(s) 85, 164, 192, 428
Mva1269I GAATGC 1 cut(s) 74
MvaI CCWGG 2 cut(s) 85, 192
MwoI GCNNNNNNNGC 3 cut(s) 80, 266, 385
NciI CCSGG 2 cut(s) 164, 428
NdeII GATC 1 cut(s) 57
NheI GCTAGC 1 cut(s) 256
NlaIII CATG 1 cut(s) 371
NlaIV GGNNCC 3 cut(s) 59, 89, 336
NmeAIII GCCGAG 2 cut(s) 130, 319
NsiI ATGCAT 1 cut(s) 76
PaeR7I CTCGAG 1 cut(s) 401
PceI AGGCCT 1 cut(s) 83
PciSI GCTCTTC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 1 cut(s) 74
PdmI GAANNNNTTC 1 cut(s) 134
PflFI GACNNNGTC 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 426
PkrI GCNGC 4 cut(s) 92, 212, 309, 312
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
PpuMI RGGWCCY 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 335
Psp6I CCWGG 2 cut(s) 83, 190
PspGI CCWGG 2 cut(s) 83, 190
PspN4I GGNNCC 3 cut(s) 59, 89, 336
PspPI GGNCC 3 cut(s) 88, 175, 335
PspPPI RGGWCCY 1 cut(s) 335
PsuI RGATCY 1 cut(s) 57
PsyI GACNNNGTC 1 cut(s) 8
RsaI GTAC 2 cut(s) 366, 412
RsaNI GTAC 2 cut(s) 365, 411
SapI GCTCTTC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 275
SatI GCNGC 4 cut(s) 91, 211, 308, 311
Sau3AI GATC 1 cut(s) 57
Sau96I GGNCC 3 cut(s) 88, 175, 335
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 4 cut(s) 85, 164, 192, 428
SduI GDGCHC 1 cut(s) 381
SetI ASST 8 cut(s) 137, 196, 300, 340, 378, 390, 410, 435
SfaNI GCATC 1 cut(s) 243
Sfr274I CTCGAG 1 cut(s) 401
SinI GGWCC 2 cut(s) 175, 335
SlaI CTCGAG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 3 cut(s) 20, 169, 276
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 2 cut(s) 91, 307
SspMI CTAG 2 cut(s) 152, 257
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 4 cut(s) 83, 162, 190, 426
StyI CCWWGG 2 cut(s) 338, 441
TaaI ACNGT 2 cut(s) 179, 364
TaqI TCGA 3 cut(s) 78, 198, 402
TasI AATT 3 cut(s) 20, 169, 276
TatI WGTACW 2 cut(s) 364, 410
TauI GCSGC 2 cut(s) 93, 310
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TseI GCWGC 2 cut(s) 210, 310
TspDTI ATGAA 1 cut(s) 372
Tth111I GACNNNGTC 1 cut(s) 8
VneI GTGCAC 1 cut(s) 377
VpaK11BI GGWCC 2 cut(s) 175, 335
XapI RAATTY 1 cut(s) 169
XhoI CTCGAG 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 2 cut(s) 152, 257
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.