Rh7DG346400

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000800
Physical Location & Seq
Forward (+)
45880132 .. 45895672
15541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG346400.1

Sequence Viewer

Length: 6435 bp
ATGGCCTCCGCCACCGAAACTCTCCTCTCCAAGCTCGAATCCGCCGATTCCGCCGGGATTCATCGCCTCATCTCCGACTACCTAAACCCATTCGCCGACCTTCACAAAAAGAAGACCTCAACCGACCACCAAACCCTACAACGCTCTCTCGCCAAAAAATTCACCCCCTTCCTCAACCGCGCCTTAGCCATCCTCCCCAAACGGCTCGCTGATCCTTCAAAGCTAGATGCCAAATTTGCCCCCGAATTGTTCGATATTTACCGCCTCTGCCTCGACTCCATGGACGCCGTCTCCTCCCAATTTTCCGGCAAGCCCTACAAGTTTCACATCCAGAGGATCCGGTTGGTTTATGCATTCGAGGCCTGGGGCCGCCACAAGGAGGCCGAGTCCGAGGCGCTTAGGGTTTTGAAGAGCTTCAAGGCGATTGAACTAGGAACGAAACCGGTGAATTTGGACCGTAGATTTGTGCCAGGTGTCGAGCAGGGAGGCTGCGATAAGGAGTTTGGGGCTTTGCTCGGAGAAGTGGTGTGGATGCTAGCCAAGTGTGCTTCGTTAATTCAAAGCAAGGAAGCCGATGTGTATGAGCGGCTGCTTTGTATGGTTGAGGAGTTGGGACCTTGGTTGAGGGTGTTAGATGAAACTGTACATGACAAGGTGCACAGACAGCTTGCCATTTATCTCGAGAGGTGTACTCTTTTTTTAGTCCGGGAGCTATCATCCTTGGGAGCAAATCTCGTATGCAAGTTCTGCCGCATAACAGTAAGCGAGTATGCAAAGTCATCCATCAAAGATCATATGTTCAAGTGCGCCCGTCGAATATGTTCAGCTGTATTTTCATTACAAGAGACTAGTTCCTCATTGATCAGTAACACATTGTTATGCTTGTTTGACTCCCTTGTTGATGAATGCAAGGCTGAGGTCGAATATTCAGGAAAAGATTTTATTGAACTTATTGCTTATTGTGCCAATAAGTGTCGAACTGCAAGTACAAATCTTTCTGGTACTATGAGATCTCATTCAAAAAAATTAATAACTCACTTTGATCAGGATCCACCTTTAAATTTGATTCTAAGACTTTATGCTACTGGTTTGCACTACGTTGATCGTAGTATAAAGTCAAGGGATGAGGATCTTACATCTTCTGGAGGTGCAATTAGAATGTTGCTTGATGATGAGGACACAATGACTGATTTGTCAGATTGTCTTGCTTCATTAGAAAGTTATTTCCAAATTGGTACAAATGATATTTCCAAAAGTGGGTTTTCCACGAGTCACGTGCAGAAGGATGTAAAGGATTATCTTCTGCCGTATTTTAATGCATTGGAGTTCTTATGCCATCCACTTGCTGACTTAGTATATTCGGGAAAGAAACAGATTTTTGCTGATAATGGAGCTGCCTCTGTTTCTAGTGAACTGTGTGTTATCCAGGGTGCATTTCATCAGTTATATGATTTGTTCCTTTCATTACAAACGTACAGAATTACTTGTGAGATAGATATAGATGGATTTAAGCACAACAGCATCCTTCGTGCTTCTGTGGCTGCTTTTATACTTTCCACCAGAACGAAGCTTAACATGCAGAAGAGTGCACAAATACTGAACCATGTCATTACCAGCGAATGGATTCAACTTGATGGTCTAAAGCATATATATGTCTCTCTCTACAATACTGGTACATGTTTGTACAAAAACAAGGAGCTGAATGAGGCTTCAGAGGCTTTGAAGCTTTCCTGCAAGGCATCTTGGACTCGTGTTATACATCTCTGTGAACTGTTTGCAGGCAAATTAAAGGCCGCCAGTGCTGATCCTTCTGAAGATACTATTTTAGATGTCTTGAATGAGTCGTGTACAGGAAGTGCTTTTCTATTGGATATTCTTAACCAAGTTGATAATCATGAGGTGAAAAGAGCTATGGCAGAGTGCCTTGAAACTTGGTCTATTGCAGCAAATTTGTTTGGGAGGCTGCCAGGTCCAATGTCTGTGGTGAAGCAGTGGGTTAAGATGGAATGTAAATGCTGTAACAATATGCATATTGAGGATAGCGGTCCAACCTTATACAGTCTGCTGTCATCTTCTAAGAAAATATCAAAGAAGACAACTGAGATAATCTTGGAGCAGGGACTTCTTGCTTATGAGAAAATGTATGCTGTGAACCCTGAGTTTTGTCAGAAGAAGCAAATGCAAATTATAAACATACTACTGCAAGATGTGTATGTGACACCAGACAGTTGGTTACAAAAAGCAAGAATTTTAGTGAGAAAGGGAAGAGCGTTAAAACTTTATGGGATCAAAGGTCTAAAAGACTGTATTCATTGTTTATCAGAAGCAATATCTTCACTTAGTAAGATGTACGATAACACCAGTATCCATCAAACATCTCCTCGCCATGAATTAGCTGTGGCATATTGCTTACGCGCGCTTTGTACCCAGGAGGATGAACCACAATCAGAGAAAATGTTTCAAGATATCCGTGCTGCCCTTGATGCATGGTTGGACATTTCTACTACAGACATGTGCTCTCCAGATGACAATTGCTCCCCAGATGACAAATGTTTAATGGTGTCTGAAAATACAATAATGCTCCTATACAATATTATTGATTTGTTGTCTATCAAGGGTCACATGGATTATTACCATGATATATATAGGCTTTTGATTAGATTATTTAACTGGAAAAATATCCCGTTAGAGAAGTGCTTGGTCAGATTGTGGGAATGCAGGAGAGTTAGTCATGCCCTTTGTACTTCACCTGTTAATGATGCACTCATCATGAGCTTTTCAGATCATTTTGGTGAACTTCCAAAGTCTATTGATTTTTGGATAGATTGTCTTAAAGGATCCCAACAATTGGTAATAGGCTTCCAACATAATTTCTCATTTCTATTTGCAAATTTACCTTGGGGCCCATGCAATCATGCAAGCTTGTTCCGATGTGAAATCACAGTTGATGAAGTTAAAAAAGCTGCTCTTGAACTTATTTCAAGTGTTCCTGTATCAAGTCAGTCGGCTTATGTAGCTGCATATCTCTACTATGATTTATGTGAAAGACTAGTTTCAAATGGACAGTTAGTTGAGGCTCTATCATATGCAATGGAAGCTCACCAGTTACGCCTTGAACTATTCCAAAAGAAGTTTGCGTACTCTTTTGTACGAAAGGCTGAAAAGTACAATGAAACTAGGGATACCTATCAGAACTTTACACTCGAAGATCATAATCTGGAAGTTAAACGATCAGTTGTCCGTGAAGTTTGGTCCTTTGACACTAGTTCATGTAACTTGGAAAACTGTTATCTTAGTCCCTATAATGTACTTCAATGTTATCTTGAAAGTACTCTTCAGGTAGGGATTATCCATGAAATAGTTGGAAATGGAGCTGAGGCCGAGGTAGTTTTACAGTGGGGAAAGACTATTTCCTGCTCACAAAGCTTGCCACTATTTGCAGTTGCATTTTGTTCAGTCTTAGGAAAACTGTATCATAAGAAGCAGCTTTGGGATTTGGCAGAGAAGGAACTTCAAAGTGCCAACCAATATTTGAAGGCTAGTGCAAAAGATATTTCTTGCGTAAAGTGCAGATTGATGCAGAAAGCAACTGTTAAACTGAATCTTGGTGATTTATACCAAAGCAAATTTTATAGTTCAAGGAGTGCATCTTTAGAGAAATTGTCTTATGCTGAAAGTTTGTACAAATCAGCACTTACCAAGCTAAACCTTTCTGAGTGGCAAAATTCTGTTAGTTTCCCTGGACAAACTGTACTTAAAGAAGTTGGATATTCTGCTGGCAGTATTTCTAGTCATTTTGTGGAAACCAAGGTGGAACCAAAAAGCAAGATAGAAGCTAAAAAGTCTAGGACTAAGAATGCCCGAAAACCAGTGGGGAGGGATCAAGTTTCAGTTCCTGAGTATAATTTGAGGTCAACTCAGTCTAGATGCCAATCTTCTCAGAACCAAAGTGTAAGAGGTACTGGTGTCCAAGTTGGTCATCCAAAACATTTGAAACGAAGCACCGAGTGTGATTCTTCTGATAATTTAAGTAACAAGGACTTTCTTTTGGATTTGAAAACTTGTGAGGTTTCATATGGGTCTGACCTAACATGCATTTGCAACAAATTGAGGTGTTGGCATTGTCTTCCCATGGAAGTTATGGAATCTGGGCTACTGAAAAACTTTGTAGATTTGAAATGGGAGTATGTCCGCAGGCGTCTCTCTCTCAGGCTTCTGACAGGATTAGGAAATTGTTTGGAGAGTCAAGGTCAAATTCATGAAACAAATGAATTTATCCTGCAAAGTGTATCGATTTTAGTCAGCAGGAACCCATTTTGTATAACTACCTCATTGATTCCGTCGACTTCCTTGCTTGATTTAATGGCAAAGGAAATCCCTGGGGATGTGCTTAGTGTTGAGCGTGCGGAAGTACTGTACAGCATATGTTGGCTGTCTTTAAAGATTCGTTCCAAGAATACCAGGGTTATTTTCTCTGATCTGCCTCATATTCATCTACCAAAACTAGTGTCTTGGCTGATGTTAGCTTTTGTGCTCTGCCGTGAAGTTCCTGTACTTTTTCAGAAGGTTTCTAGATTGCTTGCTGCTATATTTTTACTTTCTGCCTCAAGTGAGCTTTTTTCTTTGACATCTTCTTGCAAAAAGCTCCATGAAAATCATTGGGCTTCTTATTTTCATCAAGCTTCAATTGGCACCCATCTCAATTACCAATTTTTCACGAAAGTATCGGAGAGGTGTAAACTCCAACACCTTTTAAACGGTGAGGGGGCACATGTTGCTGGCTCATGCTTGGTATCAGAAACACAGAATTTACATAGGGTCGCTCCAGAGTCAATTCAAGACCTTGAAGAATTTGTGACAGAATTCTTTTCAGGCCTTCCATGCACCACAATCATCTGTATAAGTGTGCTTGGAGGCCCTTATACTAGTTTGTTACAAGAATTGTTGTGTTTTCCTTCACGCATGCATGCATGGATTCTGGTTTCACGCTTGAACTCTAAGAATCAACCTATCAGTATGCTTCTGCCAGTGGATTCAGTATTAGAAGGAGATTCAGATGATGTTAGTTATACTGGAATAAAGGATTGGCACTGTCCATGGGGTTCCACTGTGGTTGATGATGTAGCTCCAGAGTTTAGATTGATATTGGAGGGGACTCATTCGTCATCTGTGAAACATCCTGTACAAGATACAAATGAGAAAAAGCTTAACTGGTGGGTGCAGAGGAAGAATTTTGATCGCCGCCTTGGTAAATTTCTGAAGAACTTGGAAGATTCATGGTTTGGTACTTGGAAACTTGTCCTACTGGGTGAATGGTCAAACTGCAAGCAGTTGGACTTGGTACATAAGAAGCTGGTGCGCAATCTGAAATCCAAATGCAAAATGGAGATAGATGAGAGTCTTTTGAAAGTCATCCTTGGGGGTTCCAAATATGCCTTCGAAGGAGGTGCACGTGTTACACAGCTTTGTCTTAAGAAAGGTTCCTACGTTAGTAAAGTTGGATATTTAGAGGAAGAGAAGTGTATGGCATCATGTAATGATTCTAATGGTGGTGAGAACTTGTCTGAGTTGGCCTCCAAACTAGTTTTAGAAGCAGTGAATGAGCTTGAAGGTTTACATTCTTCTTTAAATATAGAGCCAACAATATTAGTTGTGGACTACGAGGTGCAGATGCTTCCTTGGGAGAATTTACCAATCCTAAGAAACCAGGAGGTTTATCGCATGCCCTCTGTTGGGAGCATTTCGGCAACAGCCAATAGAAACTACCACAATCAAGATCAAGTTCAGAGTATTGTGACTTCATTTCCTTTGATAGATCCCTTGGATGCCTTTTATCTTTTGAATCCCAGTGGTGATCTAAATTATACACAAAATGAATTTGAGACATGGTTCAGAGATCAAAACTTGGAGGGGAAAGCCGGATGTGCACCTCCTGCTGAAGAATTGGCAGTAGCGTTGAAAAGCCATGATCTATTTATGTATTTTGGCCATGGATGTGGGCAACAATATATTCCTAGACGTGAGATTCAGAGACTGGAACACTGTGCCGCAACTCTCCTTATGGGATGCAGTAGTGGTTCTCTGAAACTGAATGGATGTTATGTTCCACAAGGTACTCCACTATCCTATCTGCTGGCCGGTTCTCCTGTCATTGTCGCCAATTTGTGGGATGTGACGGACAGGGATATTGACCGATTTGCAAAGTCCATGCTTGATTCTTGGTTGAAAGCAAGGTCGAGTCCCTGTGTGAGTTGTGCCCAATGCAACAAGAAAGTCACAAAAAAGAAATTGGCCACAAACCTGTCTTGTGAGCATAGGCCGAAGGTTGGATCATTCATGAGCGAAGCACGTAAAACTTGCCAGTTACCTTTCTTGATTGGAGCAGCACCAGTATGCTATGGTGTCCCAACTGGCATATGGAAAAAGGAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

2144

Amino Acids

241.79

Weight (kDa)

7.15

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 6 - 276 3.7e-105 Separase-like, TPR repeats
TPR_ESP1_2nd PF25113 286 - 459 4.7e-40 Separase-like, TPR repeats
Separin_C PF03568 1656 - 2063 4.6e-98 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2189
AasI GACNNNNNNGTC 2 cut(s) 1192, 5161
Acc16I TGCGCA 1 cut(s) 5360
AccB1I GGYRCC 1 cut(s) 4691
AccB7I CCANNNNNTGG 3 cut(s) 1620, 2848, 6165
AccBSI CCGCTC 1 cut(s) 586
AccI GTMKAC 1 cut(s) 4343
AccII CGCG 3 cut(s) 180, 2415, 2417
AcoI YGGCCR 3 cut(s) 5986, 6135, 6291
AcuI CTGAAG 5 cut(s) 1695, 1833, 3320, 5279, 5958
AcvI CACGTG 2 cut(s) 1276, 5453
AcyI GRCGYC 2 cut(s) 285, 4198
AdeI CACNNNGTG 1 cut(s) 4008
AfiI CCNNNNNNNGG 9 cut(s) 376, 379, 1257, 1620, 2848, 5732, 5733, 6165, 6326
AflII CTTAAG 1 cut(s) 5471
AflIII ACRYGT 4 cut(s) 1676, 2511, 4771, 5452
AgeI ACCGGT 1 cut(s) 442
AhlI ACTAGT 6 cut(s) 848, 3049, 3263, 4504, 4925, 5581
AjiI CACGTC 1 cut(s) 6020
AjnI CCWGG 9 cut(s) 362, 469, 1425, 1966, 2427, 3739, 4378, 4460, 5706
AjuI GAANNNNNNNTTGG 4 cut(s) 5068, 5100, 5420, 5452
AloI GAACNNNNNNTCC 2 cut(s) 6087, 6119
Alw21I GWGCWC 6 cut(s) 660, 1591, 2519, 4536, 5452, 5929
Alw26I GTCTC 6 cut(s) 295, 839, 1660, 4205, 5876, 6025
Alw44I GTGCAC 4 cut(s) 656, 1587, 5448, 5925
AlwNI CAGNNNCTG 2 cut(s) 3896, 6034
Ama87I CYCGRG 1 cut(s) 680
ApaI GGGCCC 1 cut(s) 2906
ApaLI GTGCAC 4 cut(s) 656, 1587, 5448, 5925
ArsI GACNNNNNNTTYG 4 cut(s) 446, 478, 4493, 4525
AseI ATTAAT 1 cut(s) 1028
AsiGI ACCGGT 1 cut(s) 442
Asp700I GAANNNNTTC 3 cut(s) 413, 820, 1623
AspLEI GCGC 6 cut(s) 182, 397, 809, 2417, 2419, 5361
AspS9I GGNCC 9 cut(s) 367, 454, 614, 1970, 2045, 2902, 2903, 3252, 4916
AsuC2I CCSGG 2 cut(s) 55, 707
AsuII TTCGAA 1 cut(s) 5439
AsuNHI GCTAGC 1 cut(s) 535
AvaI CYCGRG 1 cut(s) 680
AvaII GGWCC 5 cut(s) 454, 614, 1970, 2045, 3252
BaeGI GKGCMC 7 cut(s) 660, 1591, 2906, 4771, 5452, 5929, 6259
BaeI ACNNNNGTAYC 6 cut(s) 2347, 2380, 3951, 3984, 4707, 4740
BalI TGGCCA 2 cut(s) 5988, 6293
BamHI GGATCC 3 cut(s) 336, 1048, 2837
BanI GGYRCC 1 cut(s) 4691
BanII GRGCYC 1 cut(s) 2906
BarI GAAGNNNNNNTAC 6 cut(s) 3122, 3154, 4536, 4568, 5782, 5814
BauI CACGAG 2 cut(s) 1267, 1749
BbrPI CACGTG 2 cut(s) 1276, 5453
BbsI GAAGAC 3 cut(s) 119, 2099, 4118
Bbv12I GWGCWC 6 cut(s) 660, 1591, 2519, 4536, 5452, 5929
BbvCI CCTCAGC 2 cut(s) 915, 3375
BccI CCATC 8 cut(s) 197, 791, 1344, 1496, 1628, 1996, 2376, 4704
BceAI ACGGC 4 cut(s) 218, 272, 1291, 4524
BcgI CGANNNNNNTGC 6 cut(s) 1258, 1292, 4333, 4367, 5429, 5463
BciT130I CCWGG 9 cut(s) 364, 471, 1427, 1968, 2429, 3741, 4380, 4462, 5708
BciVI GTATCC 2 cut(s) 2375, 3175
BclI TGATCA 2 cut(s) 861, 1042
BcnI CCSGG 2 cut(s) 55, 707
BcoDI GTCTC 6 cut(s) 295, 839, 1660, 4205, 5876, 6025
BcuI ACTAGT 6 cut(s) 848, 3049, 3263, 4504, 4925, 5581
BfmI CTRYAG 1 cut(s) 2505
BfoI RGCGCY 1 cut(s) 398
BfrI CTTAAG 1 cut(s) 5471
BfuI GTATCC 2 cut(s) 2375, 3175
BglII AGATCT 1 cut(s) 1010
BmcAI AGTACT 2 cut(s) 3331, 4413
Bme18I GGWCC 5 cut(s) 454, 614, 1970, 2045, 3252
BmeT110I CYCGRG 1 cut(s) 680
BmgBI CACGTC 1 cut(s) 6020
BmgT120I GGNCC 9 cut(s) 367, 454, 614, 1970, 2045, 2902, 2903, 3252, 4916
BmrI ACTGGG 2 cut(s) 5315, 5841
BmtI GCTAGC 1 cut(s) 539
BmuI ACTGGG 2 cut(s) 5315, 5841
BpiI GAAGAC 3 cut(s) 119, 2099, 4118
BplI GAGNNNNNCTC 8 cut(s) 676, 708, 717, 749, 3901, 3933, 5558, 5590
BpmI CTGGAG 4 cut(s) 1164, 2505, 4809, 5112
Bpu10I CCTNAGC 4 cut(s) 184, 398, 915, 3375
Bpu14I TTCGAA 1 cut(s) 5439
BpuEI CTTGAG 1 cut(s) 4591
BpuMI CCSGG 2 cut(s) 55, 707
Bsa29I ATCGAT 1 cut(s) 4292
BsaAI YACGTR 3 cut(s) 1276, 5453, 6350
BsaBI GATNNNNATC 5 cut(s) 1047, 1128, 3186, 3213, 3931
BsaHI GRCGYC 2 cut(s) 285, 4198
BsaWI WCCGGW 2 cut(s) 339, 442
Bsc4I CCNNNNNNNGG 9 cut(s) 376, 379, 1257, 1620, 2848, 5732, 5733, 6165, 6326
Bse118I RCCGGY 2 cut(s) 442, 6137
Bse3DI GCAATG 1 cut(s) 3096
Bse8I GATNNNNATC 5 cut(s) 1047, 1128, 3186, 3213, 3931
BseBI CCWGG 9 cut(s) 364, 471, 1427, 1968, 2429, 3741, 4380, 4462, 5708
BseCI ATCGAT 1 cut(s) 4292
BseJI GATNNNNATC 5 cut(s) 1047, 1128, 3186, 3213, 3931
BseLI CCNNNNNNNGG 9 cut(s) 376, 379, 1257, 1620, 2848, 5732, 5733, 6165, 6326
BseMI GCAATG 1 cut(s) 3096
BsePI GCGCGC 1 cut(s) 2415
BseRI GAGGAG 4 cut(s) 14, 283, 620, 2370
BseSI GKGCMC 7 cut(s) 660, 1591, 2906, 4771, 5452, 5929, 6259
BsgI GTGCAG 4 cut(s) 1298, 3589, 5240, 5687
Bsh1236I CGCG 3 cut(s) 180, 2415, 2417
BshNI GGYRCC 1 cut(s) 4691
BshTI ACCGGT 1 cut(s) 442
BshVI ATCGAT 1 cut(s) 4292
BsiHKAI GWGCWC 6 cut(s) 660, 1591, 2519, 4536, 5452, 5929
BsiHKCI CYCGRG 1 cut(s) 680
BsiSI CCGG 7 cut(s) 54, 306, 340, 443, 706, 5919, 6138
BslFI GGGAC 6 cut(s) 627, 2133, 3282, 5167, 6225, 6389
BslI CCNNNNNNNGG 9 cut(s) 376, 379, 1257, 1620, 2848, 5732, 5733, 6165, 6326
BsmAI GTCTC 6 cut(s) 295, 839, 1660, 4205, 5876, 6025
BsmBI CGTCTC 2 cut(s) 295, 4205
BsmFI GGGAC 6 cut(s) 627, 2133, 3282, 5167, 6225, 6389
BsmI GAATGC 4 cut(s) 353, 911, 2720, 3862
BsoBI CYCGRG 1 cut(s) 680
Bsp119I TTCGAA 1 cut(s) 5439
Bsp120I GGGCCC 1 cut(s) 2902
Bsp1286I GDGCHC 9 cut(s) 660, 1591, 2519, 2906, 4536, 4771, 5452, 5929, 6259
Bsp1407I TGTACA 6 cut(s) 643, 1683, 1847, 3681, 4416, 5182
Bsp19I CCATGG 4 cut(s) 279, 4131, 5096, 5989
BspDI ATCGAT 1 cut(s) 4292
BspFNI CGCG 3 cut(s) 180, 2415, 2417
BspHI TCATGA 4 cut(s) 1894, 2769, 4258, 6336
BspOI GCTAGC 1 cut(s) 539
BspQI GCTCTTC 2 cut(s) 404, 2260
BspT104I TTCGAA 1 cut(s) 5439
BspT107I GGYRCC 1 cut(s) 4691
BspTI CTTAAG 1 cut(s) 5471
BsrBI CCGCTC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 3096
BsrFI RCCGGY 2 cut(s) 442, 6137
BsrGI TGTACA 6 cut(s) 643, 1683, 1847, 3681, 4416, 5182
BssAI RCCGGY 2 cut(s) 442, 6137
BssHII GCGCGC 1 cut(s) 2415
BssNI GRCGYC 2 cut(s) 285, 4198
BssSI CACGAG 2 cut(s) 1267, 1749
Bst2BI CACGAG 2 cut(s) 1267, 1749
Bst2UI CCWGG 9 cut(s) 364, 471, 1427, 1968, 2429, 3741, 4380, 4462, 5708
Bst6I CTCTTC 5 cut(s) 404, 1577, 2260, 3339, 5508
BstACI GRCGYC 2 cut(s) 285, 4198
BstAFI CTTAAG 1 cut(s) 5471
BstAPI GCANNNNNTGC 3 cut(s) 747, 4775, 5933
BstAUI TGTACA 6 cut(s) 643, 1683, 1847, 3681, 4416, 5182
BstBAI YACGTR 3 cut(s) 1276, 5453, 6350
BstBI TTCGAA 1 cut(s) 5439
BstDSI CCRYGG 4 cut(s) 279, 4131, 5096, 5989
BstFNI CGCG 3 cut(s) 180, 2415, 2417
BstH2I RGCGCY 1 cut(s) 398
BstHHI GCGC 6 cut(s) 182, 397, 809, 2417, 2419, 5361
BstMAI GTCTC 6 cut(s) 295, 839, 1660, 4205, 5876, 6025
BstNI CCWGG 9 cut(s) 364, 471, 1427, 1968, 2429, 3741, 4380, 4462, 5708
BstNSI RCATGY 8 cut(s) 1579, 1680, 2515, 4095, 4775, 4966, 4970, 5725
BstSFI CTRYAG 1 cut(s) 2505
BstSLI GKGCMC 7 cut(s) 660, 1591, 2906, 4771, 5452, 5929, 6259
BstUI CGCG 3 cut(s) 180, 2415, 2417
BstV2I GAAGAC 3 cut(s) 119, 2099, 4118
BstX2I RGATCY 6 cut(s) 336, 1010, 1048, 1129, 2837, 5815
BstXI CCANNNNNNTGG 2 cut(s) 2229, 5996
BstYI RGATCY 6 cut(s) 336, 1010, 1048, 1129, 2837, 5815
Bsu15I ATCGAT 1 cut(s) 4292
BsuI GTATCC 2 cut(s) 2375, 3175
BsuTUI ATCGAT 1 cut(s) 4292
BtgI CCRYGG 4 cut(s) 279, 4131, 5096, 5989
BtgZI GCGATG 1 cut(s) 47
BtrI CACGTC 1 cut(s) 6020
BtsI GCAGTG 2 cut(s) 1997, 5601
CaiI CAGNNNCTG 2 cut(s) 3896, 6034
CciI TCATGA 4 cut(s) 1894, 2769, 4258, 6336
CfoI GCGC 6 cut(s) 182, 397, 809, 2417, 2419, 5361
Cfr10I RCCGGY 2 cut(s) 442, 6137
Cfr13I GGNCC 9 cut(s) 367, 454, 614, 1970, 2045, 2902, 2903, 3252, 4916
ClaI ATCGAT 1 cut(s) 4292
CseI GACGC 2 cut(s) 293, 4187
CspAI ACCGGT 1 cut(s) 442
CspCI CAANNNNNGTGG 4 cut(s) 1962, 1997, 5095, 5130
DraI TTTAAA 4 cut(s) 1059, 4440, 4755, 5628
DraIII CACNNNGTG 1 cut(s) 4008
DrdI GACNNNNNNGTC 2 cut(s) 1192, 5161
DseDI GACNNNNNNGTC 2 cut(s) 1192, 5161
EaeI YGGCCR 3 cut(s) 5986, 6135, 6291
Eam1104I CTCTTC 5 cut(s) 404, 1577, 2260, 3339, 5508
EarI CTCTTC 5 cut(s) 404, 1577, 2260, 3339, 5508
EciI GGCGGA 2 cut(s) 31, 40
Eco147I AGGCCT 2 cut(s) 362, 4875
Eco24I GRGCYC 1 cut(s) 2906
Eco32I GATATC 1 cut(s) 2467
Eco47I GGWCC 5 cut(s) 454, 614, 1970, 2045, 3252
Eco57I CTGAAG 5 cut(s) 1695, 1833, 3320, 5279, 5958
Eco72I CACGTG 2 cut(s) 1276, 5453
Eco88I CYCGRG 1 cut(s) 680
EcoO109I RGGNCCY 3 cut(s) 614, 2902, 4916
EcoRI GAATTC 1 cut(s) 4862
EcoRII CCWGG 9 cut(s) 362, 469, 1425, 1966, 2427, 3739, 4378, 4460, 5706
EcoRV GATATC 1 cut(s) 2467
EcoT22I ATGCAT 7 cut(s) 355, 1321, 2031, 2488, 4097, 4968, 4972
EcoT38I GRGCYC 1 cut(s) 2906
Esp3I CGTCTC 2 cut(s) 295, 4205
FalI AAGNNNNNCTT 4 cut(s) 2952, 2984, 5400, 5432
FaqI GGGAC 6 cut(s) 627, 2133, 3282, 5167, 6225, 6389
FauNDI CATATG 5 cut(s) 795, 3085, 4075, 4424, 6416
FbaI TGATCA 2 cut(s) 861, 1042
FblI GTMKAC 1 cut(s) 4343
FriOI GRGCYC 1 cut(s) 2906
FspI TGCGCA 1 cut(s) 5360
GlaI GCGC 6 cut(s) 181, 396, 808, 2416, 2418, 5360
GsuI CTGGAG 4 cut(s) 1164, 2505, 4809, 5112
HaeII RGCGCY 1 cut(s) 398
HapII CCGG 7 cut(s) 54, 306, 340, 443, 706, 5919, 6138
HgaI GACGC 2 cut(s) 293, 4187
HhaI GCGC 6 cut(s) 182, 397, 809, 2417, 2419, 5361
Hin1I GRCGYC 2 cut(s) 285, 4198
Hin6I GCGC 6 cut(s) 180, 395, 807, 2415, 2417, 5359
HinP1I GCGC 6 cut(s) 180, 395, 807, 2415, 2417, 5359
HincII GTYRAC 2 cut(s) 3915, 4344
HindII GTYRAC 2 cut(s) 3915, 4344
HindIII AAGCTT 6 cut(s) 1568, 1724, 2920, 3424, 4680, 5204
HpaII CCGG 7 cut(s) 54, 306, 340, 443, 706, 5919, 6138
Hpy99I CGWCG 2 cut(s) 816, 4345
HpyCH4IV ACGT 7 cut(s) 1098, 1275, 1472, 5452, 5487, 6019, 6349
HpySE526I ACGT 7 cut(s) 1098, 1275, 1472, 5452, 5487, 6019, 6349
Hsp92I GRCGYC 2 cut(s) 285, 4198
HspAI GCGC 6 cut(s) 180, 395, 807, 2415, 2417, 5359
Ksp22I TGATCA 2 cut(s) 861, 1042
LguI GCTCTTC 2 cut(s) 404, 2260
MaeII ACGT 7 cut(s) 1098, 1275, 1472, 5452, 5487, 6019, 6349
MbiI CCGCTC 1 cut(s) 586
MfeI CAATTG 3 cut(s) 2530, 2846, 4686
MflI RGATCY 6 cut(s) 336, 1010, 1048, 1129, 2837, 5815
MhlI GDGCHC 9 cut(s) 660, 1591, 2519, 2906, 4536, 4771, 5452, 5929, 6259
MlsI TGGCCA 2 cut(s) 5988, 6293
MluNI TGGCCA 2 cut(s) 5988, 6293
Mox20I TGGCCA 2 cut(s) 5988, 6293
Mph1103I ATGCAT 7 cut(s) 355, 1321, 2031, 2488, 4097, 4968, 4972
MroXI GAANNNNTTC 3 cut(s) 413, 820, 1623
MscI TGGCCA 2 cut(s) 5988, 6293
MslI CAYNNNNRTG 6 cut(s) 877, 1650, 1764, 2790, 5994, 6391
Msp20I TGGCCA 2 cut(s) 5988, 6293
MspA1I CMGCKG 1 cut(s) 827
MspCI CTTAAG 1 cut(s) 5471
MspI CCGG 7 cut(s) 54, 306, 340, 443, 706, 5919, 6138
MunI CAATTG 3 cut(s) 2530, 2846, 4686
Mva1269I GAATGC 4 cut(s) 353, 911, 2720, 3862
MvaI CCWGG 9 cut(s) 364, 471, 1427, 1968, 2429, 3741, 4380, 4462, 5708
MvnI CGCG 3 cut(s) 180, 2415, 2417
NciI CCSGG 2 cut(s) 55, 707
NcoI CCATGG 4 cut(s) 279, 4131, 5096, 5989
NdeI CATATG 5 cut(s) 795, 3085, 4075, 4424, 6416
NheI GCTAGC 1 cut(s) 535
NmeAIII GCCGAG 2 cut(s) 409, 3406
NmuCI GTSAC 7 cut(s) 1271, 2215, 2618, 4855, 5794, 6172, 6274
NsbI TGCGCA 1 cut(s) 5360
NsiI ATGCAT 7 cut(s) 355, 1321, 2031, 2488, 4097, 4968, 4972
NspI RCATGY 8 cut(s) 1579, 1680, 2515, 4095, 4775, 4966, 4970, 5725
NspV TTCGAA 1 cut(s) 5439
PaeI GCATGC 3 cut(s) 4966, 4970, 5725
PaeR7I CTCGAG 1 cut(s) 680
PagI TCATGA 4 cut(s) 1894, 2769, 4258, 6336
PasI CCCWGGG 1 cut(s) 4379
PauI GCGCGC 1 cut(s) 2415
PceI AGGCCT 2 cut(s) 362, 4875
PciI ACATGT 3 cut(s) 1676, 2511, 4771
PciSI GCTCTTC 2 cut(s) 404, 2260
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PctI GAATGC 4 cut(s) 353, 911, 2720, 3862
PdmI GAANNNNTTC 3 cut(s) 413, 820, 1623
PflFI GACNNNGTC 1 cut(s) 287
PflMI CCANNNNNTGG 3 cut(s) 1620, 2848, 6165
PfoI TCCNGGA 1 cut(s) 705
PinAI ACCGGT 1 cut(s) 442
PmaCI CACGTG 2 cut(s) 1276, 5453
PmlI CACGTG 2 cut(s) 1276, 5453
Ppu21I YACGTR 3 cut(s) 1276, 5453, 6350
PpuMI RGGWCCY 1 cut(s) 614
PscI ACATGT 3 cut(s) 1676, 2511, 4771
PshBI ATTAAT 1 cut(s) 1028
PsiI TTATAA 1 cut(s) 2189
Psp5II RGGWCCY 1 cut(s) 614
Psp6I CCWGG 9 cut(s) 362, 469, 1425, 1966, 2427, 3739, 4378, 4460, 5706
PspCI CACGTG 2 cut(s) 1276, 5453
PspGI CCWGG 9 cut(s) 362, 469, 1425, 1966, 2427, 3739, 4378, 4460, 5706
PspOMI GGGCCC 1 cut(s) 2902
PspPI GGNCC 9 cut(s) 367, 454, 614, 1970, 2045, 2902, 2903, 3252, 4916
PspPPI RGGWCCY 1 cut(s) 614
PsrI GAACNNNNNNTAC 2 cut(s) 970, 1002
PstNI CAGNNNCTG 2 cut(s) 3896, 6034
PsuI RGATCY 6 cut(s) 336, 1010, 1048, 1129, 2837, 5815
PsyI GACNNNGTC 1 cut(s) 287
PteI GCGCGC 1 cut(s) 2415
PvuII CAGCTG 1 cut(s) 827
RseI CAYNNNNRTG 6 cut(s) 877, 1650, 1764, 2790, 5994, 6391
SalI GTCGAC 1 cut(s) 4342
SapI GCTCTTC 2 cut(s) 404, 2260
Sau96I GGNCC 9 cut(s) 367, 454, 614, 1970, 2045, 2902, 2903, 3252, 4916
ScaI AGTACT 2 cut(s) 3331, 4413
SduI GDGCHC 9 cut(s) 660, 1591, 2519, 2906, 4536, 4771, 5452, 5929, 6259
SfcI CTRYAG 1 cut(s) 2505
Sfr274I CTCGAG 1 cut(s) 680
SfuI TTCGAA 1 cut(s) 5439
SinI GGWCC 5 cut(s) 454, 614, 1970, 2045, 3252
SlaI CTCGAG 1 cut(s) 680
SmiMI CAYNNNNRTG 6 cut(s) 877, 1650, 1764, 2790, 5994, 6391
SmlI CTYRAG 3 cut(s) 680, 4606, 5471
SmoI CTYRAG 3 cut(s) 680, 4606, 5471
SpeI ACTAGT 6 cut(s) 848, 3049, 3263, 4504, 4925, 5581
SphI GCATGC 3 cut(s) 4966, 4970, 5725
SseBI AGGCCT 2 cut(s) 362, 4875
SspI AATATT 4 cut(s) 926, 2593, 3530, 5646
StuI AGGCCT 2 cut(s) 362, 4875
TaiI ACGT 7 cut(s) 1101, 1278, 1475, 5455, 5490, 6022, 6352
TaqII GACCGA 1 cut(s) 6207
TauI GCSGC 6 cut(s) 372, 589, 753, 1796, 5244, 6050
TseFI GTSAC 7 cut(s) 1271, 2215, 2618, 4855, 5794, 6172, 6274
Tsp45I GTSAC 7 cut(s) 1271, 2215, 2618, 4855, 5794, 6172, 6274
TspGWI ACGGA 4 cut(s) 2459, 3230, 4329, 6191
Tth111I GACNNNGTC 1 cut(s) 287
Van91I CCANNNNNTGG 3 cut(s) 1620, 2848, 6165
Vha464I CTTAAG 1 cut(s) 5471
VneI GTGCAC 4 cut(s) 656, 1587, 5448, 5925
VpaK11BI GGWCC 5 cut(s) 454, 614, 1970, 2045, 3252
VspI ATTAAT 1 cut(s) 1028
XbaI TCTAGA 2 cut(s) 3923, 4571
XceI RCATGY 8 cut(s) 1579, 1680, 2515, 4095, 4775, 4966, 4970, 5725
XcmI CCANNNNNNNNNTGG 4 cut(s) 3359, 4138, 5380, 6414
XhoI CTCGAG 1 cut(s) 680
XmiI GTMKAC 1 cut(s) 4343
XmnI GAANNNNTTC 3 cut(s) 413, 820, 1623
ZrmI AGTACT 2 cut(s) 3331, 4413
Zsp2I ATGCAT 7 cut(s) 355, 1321, 2031, 2488, 4097, 4968, 4972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.