Rh7DG010300

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000800
Physical Location & Seq
Forward (+)
870998 .. 879833
8836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG010300.1

Sequence Viewer

Length: 3255 bp
ATGACCTCCTCCGAAGCCGACGTTCTAATTTCCGACCTCTCCACAGGTAAAGCAGGGATTCATGGACGCATTTCGGCCTACTTACTTGTGTTCAAAGAACCCCTCAAAACCCGGTCCCAAAAGCAATCTCTTGTGACCGATTACTACCCCTTCCTCAACCGAGCGCTTACTCTTCTCCCACGAAAGCTAGGTCATCCTTCAAAGTTGAATGATAAATTCGCCGCCGAATGGTTCGCTGTTTACCGACTGTGCTTCGGCTGTATTGCCGTCATCTCCAGGATTCTAACGAAACCACTCCTGCTCCTTGGTGAAGGGTTCAAGATGCTGCGGTGCTTCTCGCGTTGGCGTCGTTGGGAGGATGGCGAGAGGGAGGGACTAGCAATTTTGAAGGACATCAAGGAGATGCAACGCCTCCCTGTCAGTGACGTCACATCCAACTTATATTTGCCGGATTTGGATGCCGATGCCGATGATGCAGGGGAAGCAACATTGCTTGCCAAAACGGTGTACTTCCTGTTTGTTTTCGCGGCCAATAAGAAGACCAGGCATCCTGACCAGTATCACCGCCTTTATCATATTGCTACCGAGTTTCAAACATGGACCAGGGTATTGGATAAACATCTCCGCCAGCTCGTGCTCGAGGAGCAAGCGAGCTATCTTATTGCGACTTGTACCATCTTTTTAGTTGGCGAGAAAGTGTTTCAAGATATTCAGTCTGCCCTTGCTGTGTGGTCCAGCATTCCTACTTCAGATGGCTCCATGGTGCCTGAAAATATAATGAAGATACTTTATTCTGTAATTGATTTGCTATCCCTCAAGGGTCGCGTAGACTTTCACTGTACTATATACAAGGTTATGATTAAGATCTATACCCAGAGAGGAGTCTCGTTGAATAATTTGTTGGCCAAATTGTGGGAATCCAGAAGGATCAGTCATGCACTCTGCCCTTCTCCAGTTCAAGAGGCATTTATGAACCTGTCAGATGTTTTTGGCGAAAAGTTAAAGTTAACTGAGTTTTGGATAGAGTGTTTTAGTAAATCCCAGCCATTGGTAGTAGGGTTTCATAACAATTTCTCATCTTCTCAATCCAATATAACAGTTCATAATGTTGAAGAAGTAACTTCTGCACTAATTTCAAGTGTTTCTGAATCTGGTTCTTACCTAGCTGGATATATGTACTACGATTTGTGTGAAAAATTGGTTTCAAATGGGCGTTCAATTGAGGCTCTTCCGTATGCAATGGAAGCCCACCGCTTACGAGTAGAACTATTCAAACAGAAATTTCCATGGTCGTTTGAAGGACGCGGTGAAGGGAATCACGTGCTAGTCTGTAGATCAGTTGCTAGTGAACTATGGTCCTTTGATACTAATTCATGTGACTTGGACACCTGCTATATTAGCCCCTGGAATGCACTTCAGTGCTATCTTGAGAGCATTCTTCAGGTAGGGGTTATCCATGAAATACTGGGAAATGGAGCTGAGGCCGAATATTTTTTGGATCGGGGAAAAAGTCTTTCCTGCTCACATAGCTTGACTCTTTTTAACATTGTTTTTTCTTCCTTTTTGGGAGAACTATACCATAAGAAGCAGCGTTGCGCTTCAGCAAAAAAGGAACTTCAAAGCTCCAGGGAACTCTTGAAGGATGGCATAATAGATGGTTTTTGCTCAAAATGCAAAGTGATACTTGAAGTAACTGTTACACATAATCTTGCTGATTTATACCAAAGCCCCATCAGTGAAATTGAGAGTGCATCTGCAGAGAAATTATCTCCTGATCTCTACAAAGAAACTCTAGCCAAGCTACATACTTCTGAGTGGAAAAAGTCTATTGCTTGTCTTGAAGAACAAGATGGTACTATTTTAGTCAAAGATGGTAATTCACAACTTGAAGGCAGTATTGAGTGCAATTCTCCCAATACATTGTGTACGAAGGAACTTCCCTGGGATTTGGATTTGCAAAACTGTGTAGTATTATTTGGGGGTGATGTACCATGCATATGCAAAACAATGGGGTGTTTGCATTGTCTTACAGGGAAAGTAACAAAATCTGGCTTAGTGACTGATGCTATAAAGTTGAAGTGGGAACTAGTACACCGTCGTCTTTCACTCAAGCTGCTCACAGGATTAGGAAAACGCTTGGAGAATGATGGCCAAATTCATGAAGCATGTGAAATTATATTTCAAAGCATATCAGTATTAGTTGGACCAAACCCATTTTGTCGCGCAAGCAAATCAACTTTCTTGCCTGATACGATGGGAAAGGAAATCAGTGCTCAGCGGAAACATATAAACCTATATCATCTAATGGGAAGGGAAATCCCAGGAGATATGCTCAATGTTGAGCGTGCTGTAATACTTTACAACCTATGTTGGTTGTCTTTGAAGGACCGTAAGAAGGATACCAGCAATAATCCCCATGTGCTGATTGATATTCCTATATCAAAACTAGTGTCTTGGTTGATGCTTTCATTCGTCCTCTGCCGTGAGGTTCCTGCACTTTTTCAGAAGGTTTCAAGATTGCTGGCTGCTATTTTCTCACTGTCTACCTCAAGTGTGCTTGAAGCTCCATTGTCAACTTCTTGCAAAGATCTCTGTGGACGTCATTGGGTTTGTTTTTTTCATCAAGCTTCACTTGGAATTCAGTTTAATTACCTGTTTTCCATGAAAGAAGTCCAGCAGGGTGCAGACACTAAGGGTTCACACATGGCATCAGGAGCACAGAGTTTACCTAGGCTCGCACCAGAATCTATTCTAGACCTTAAAGACTTTGTAAAAGAATTTTTTGAAGGCCTTCCTGGCACCACAGTCATCTGTCTAAGTTTACTTGGCTGCCCATATACTAGTATGTTTCAGGAATTATTGTCTTGTTCATCTGCTCCTGCATGGATTATGGTGTCTCGCTTAAACCCTAAGAATCTTCCTATACATATTCTTCTTCCGGTGCATTCAGTACCAGGAGGAGATTCAGATGGTGTTACAACTTCTGGTGAGTATGATGGTGGTCAGTGGCGCTGTCCATGGGGTCCCACTGTGGTTGATGATGTAGCTCCGAATTTTAGAATGCTATTGGAGGAGAATTTCAATTTATCAAATTACGAGTTAGCTTTAAGTCCTGCTGAAAAGAAAGATCATATATATAATGTGTGGGAAGCGAGACGGAGGATTGATGATCGCCTTTGTACATTGCTGAAGTGTGTTATACTCTCCTCCTCTCTATGTAATTTTGGTCTTCTACTATATTTTATTTTTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

1084

Amino Acids

121.86

Weight (kDa)

6.58

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 8 - 235 8.6e-48 Separase-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1397
AatII GACGTC 2 cut(s) 429, 2602
Acc36I ACCTGC 1 cut(s) 1397
AccB1I GGYRCC 2 cut(s) 763, 2798
AccB7I CCANNNNNTGG 2 cut(s) 912, 1048
AccI GTMKAC 2 cut(s) 828, 2543
AccII CGCG 5 cut(s) 340, 527, 825, 1305, 2223
AciI CCGC 8 cut(s) 222, 328, 527, 565, 625, 1252, 1305, 2278
AclWI GGATC 2 cut(s) 935, 1506
AcoI YGGCCR 3 cut(s) 528, 903, 2149
AcsI RAATTY 7 cut(s) 215, 1280, 2154, 2637, 2777, 3052, 3076
AcuI CTGAAG 5 cut(s) 732, 1400, 1424, 1584, 3209
AcvI CACGTG 1 cut(s) 1321
AcyI GRCGYC 3 cut(s) 346, 426, 2599
AfeI AGCGCT 1 cut(s) 165
AfiI CCNNNNNNNGG 4 cut(s) 228, 912, 1048, 2395
AhlI ACTAGT 3 cut(s) 2086, 2446, 2840
AjnI CCWGG 9 cut(s) 275, 542, 602, 1403, 1625, 1940, 2320, 2794, 2953
AjuI GAANNNNNNNTTGG 2 cut(s) 884, 916
AleI CACNNNNGTG 1 cut(s) 1417
Alw21I GWGCWC 3 cut(s) 639, 2275, 2719
Alw26I GTCTC 3 cut(s) 889, 2901, 3148
AlwI GGATC 2 cut(s) 935, 1506
Ama87I CYCGRG 1 cut(s) 638
Aor51HI AGCGCT 1 cut(s) 165
AoxI GGCC 6 cut(s) 75, 528, 903, 1482, 2149, 2788
ApeKI GCWGC 5 cut(s) 325, 1588, 2113, 2525, 2829
ApoI RAATTY 7 cut(s) 215, 1280, 2154, 2637, 2777, 3052, 3076
ArsI GACNNNNNNTTYG 4 cut(s) 175, 207, 2435, 2467
Asp700I GAANNNNTTC 2 cut(s) 2748, 2790
AspA2I CCTAGG 1 cut(s) 2729
AspLEI GCGC 4 cut(s) 166, 1598, 2225, 3012
AspS9I GGNCC 7 cut(s) 114, 600, 732, 1356, 2204, 2386, 3023
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 5 cut(s) 320, 554, 1319, 1994, 2999
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 7 cut(s) 114, 600, 732, 1356, 2204, 2386, 3023
AvrII CCTAGG 1 cut(s) 2729
BalI TGGCCA 2 cut(s) 905, 2151
BanI GGYRCC 2 cut(s) 763, 2798
BauI CACGAG 1 cut(s) 632
BbrPI CACGTG 1 cut(s) 1321
BbsI GAAGAC 2 cut(s) 545, 3221
Bbv12I GWGCWC 3 cut(s) 639, 2275, 2719
BbvCI CCTCAGC 1 cut(s) 1479
BbvI GCAGC 5 cut(s) 312, 1600, 2100, 2512, 2816
BceAI ACGGC 2 cut(s) 251, 2466
BciT130I CCWGG 9 cut(s) 277, 544, 604, 1405, 1627, 1942, 2322, 2796, 2955
BciVI GTATCC 1 cut(s) 2392
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 3 cut(s) 889, 2901, 3148
BcuI ACTAGT 3 cut(s) 2086, 2446, 2840
BfmI CTRYAG 2 cut(s) 1330, 1755
BfoI RGCGCY 2 cut(s) 167, 3013
BfuAI ACCTGC 1 cut(s) 1397
BfuI GTATCC 1 cut(s) 2392
BglI GCCNNNNNGGC 1 cut(s) 2796
BglII AGATCT 2 cut(s) 864, 2587
BisI GCNGC 7 cut(s) 222, 326, 528, 1589, 2114, 2526, 2830
BlnI CCTAGG 1 cut(s) 2729
BlpI GCTNAGC 1 cut(s) 2274
BlsI GCNGC 7 cut(s) 223, 327, 529, 1590, 2115, 2527, 2831
Bme18I GGWCC 7 cut(s) 114, 600, 732, 1356, 2204, 2386, 3023
BmeT110I CYCGRG 1 cut(s) 638
BmgT120I GGNCC 7 cut(s) 114, 600, 732, 1356, 2204, 2386, 3023
BmiI GGNNCC 7 cut(s) 116, 757, 765, 2490, 2800, 3024, 3025
BmrI ACTGGG 1 cut(s) 1475
BmuI ACTGGG 1 cut(s) 1475
BpiI GAAGAC 2 cut(s) 545, 3221
BplI GAGNNNNNCTC 2 cut(s) 2316, 2348
BpmI CTGGAG 3 cut(s) 259, 936, 1609
Bpu10I CCTNAGC 1 cut(s) 1479
Bpu1102I GCTNAGC 1 cut(s) 2274
BpuEI CTTGAG 4 cut(s) 800, 1448, 2093, 2533
BpuMI CCSGG 1 cut(s) 112
BsaAI YACGTR 1 cut(s) 1321
BsaBI GATNNNNATC 2 cut(s) 618, 863
BsaHI GRCGYC 3 cut(s) 346, 426, 2599
BsaWI WCCGGW 1 cut(s) 2938
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 912, 1048, 2395
Bse1I ACTGG 3 cut(s) 556, 953, 1470
Bse3DI GCAATG 3 cut(s) 488, 1245, 3182
Bse8I GATNNNNATC 2 cut(s) 618, 863
BseBI CCWGG 9 cut(s) 277, 544, 604, 1405, 1627, 1942, 2322, 2796, 2955
BseGI GGATG 6 cut(s) 193, 364, 431, 463, 547, 1648
BseJI GATNNNNATC 2 cut(s) 618, 863
BseLI CCNNNNNNNGG 4 cut(s) 228, 912, 1048, 2395
BseMI GCAATG 3 cut(s) 488, 1245, 3182
BseMII CTCAG 4 cut(s) 1002, 1470, 1803, 2288
BseNI ACTGG 3 cut(s) 556, 953, 1470
BseRI GAGGAG 6 cut(s) 656, 894, 2973, 3086, 3196, 3199
BseXI GCAGC 5 cut(s) 312, 1600, 2100, 2512, 2816
BseYI CCCAGC 1 cut(s) 1041
BsgI GTGCAG 3 cut(s) 1110, 2478, 2703
Bsh1236I CGCG 5 cut(s) 340, 527, 825, 1305, 2223
BshFI GGCC 6 cut(s) 77, 530, 905, 1484, 2151, 2790
BshNI GGYRCC 2 cut(s) 763, 2798
BsiHKAI GWGCWC 3 cut(s) 639, 2275, 2719
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 3 cut(s) 112, 449, 2939
BslFI GGGAC 3 cut(s) 100, 387, 3009
BslI CCNNNNNNNGG 4 cut(s) 228, 912, 1048, 2395
BsmAI GTCTC 3 cut(s) 889, 2901, 3148
BsmBI CGTCTC 1 cut(s) 3148
BsmFI GGGAC 3 cut(s) 100, 387, 3009
BsmI GAATGC 5 cut(s) 738, 1414, 1434, 2944, 3066
BsnI GGCC 6 cut(s) 77, 530, 905, 1484, 2151, 2790
BsoBI CYCGRG 1 cut(s) 638
Bsp1286I GDGCHC 3 cut(s) 639, 2275, 2719
Bsp1407I TGTACA 1 cut(s) 3179
Bsp143I GATC 8 cut(s) 864, 927, 1334, 1498, 1774, 2587, 3127, 3169
Bsp1720I GCTNAGC 1 cut(s) 2274
Bsp19I CCATGG 3 cut(s) 759, 1286, 3017
BspACI CCGC 8 cut(s) 222, 328, 527, 565, 625, 1252, 1305, 2278
BspANI GGCC 6 cut(s) 77, 530, 905, 1484, 2151, 2790
BspCNI CTCAG 4 cut(s) 1003, 1471, 1804, 2287
BspFNI CGCG 5 cut(s) 340, 527, 825, 1305, 2223
BspHI TCATGA 1 cut(s) 2158
BspLI GGNNCC 7 cut(s) 116, 757, 765, 2490, 2800, 3024, 3025
BspMAI CTGCAG 1 cut(s) 1759
BspMI ACCTGC 1 cut(s) 1397
BspPI GGATC 2 cut(s) 935, 1506
BspQI GCTCTTC 1 cut(s) 1233
BspT107I GGYRCC 2 cut(s) 763, 2798
BsrDI GCAATG 3 cut(s) 488, 1245, 3182
BsrGI TGTACA 1 cut(s) 3179
BsrI ACTGG 3 cut(s) 556, 953, 1470
BssMI GATC 8 cut(s) 864, 927, 1334, 1498, 1774, 2587, 3127, 3169
BssNI GRCGYC 3 cut(s) 346, 426, 2599
BssSI CACGAG 1 cut(s) 632
BssT1I CCWWGG 5 cut(s) 304, 759, 1286, 2729, 3017
Bst2BI CACGAG 1 cut(s) 632
Bst2UI CCWGG 9 cut(s) 277, 544, 604, 1405, 1627, 1942, 2322, 2796, 2955
Bst6I CTCTTC 2 cut(s) 177, 1233
BstACI GRCGYC 3 cut(s) 346, 426, 2599
BstAUI TGTACA 1 cut(s) 3179
BstBAI YACGTR 1 cut(s) 1321
BstC8I GCNNGC 8 cut(s) 495, 629, 648, 652, 2227, 2346, 2523, 2736
BstDEI CTNAG 8 cut(s) 1011, 1479, 1812, 2053, 2274, 2691, 2816, 2910
BstDSI CCRYGG 3 cut(s) 759, 1286, 3017
BstF5I GGATG 6 cut(s) 193, 364, 431, 463, 547, 1648
BstFNI CGCG 5 cut(s) 340, 527, 825, 1305, 2223
BstH2I RGCGCY 2 cut(s) 167, 3013
BstHHI GCGC 4 cut(s) 166, 1598, 2225, 3012
BstKTI GATC 8 cut(s) 867, 930, 1337, 1501, 1777, 2590, 3130, 3172
BstMAI GTCTC 3 cut(s) 889, 2901, 3148
BstMBI GATC 8 cut(s) 864, 927, 1334, 1498, 1774, 2587, 3127, 3169
BstMWI GCNNNNNNNGC 9 cut(s) 473, 482, 643, 1244, 1398, 1527, 1671, 2714, 2796
BstNI CCWGG 9 cut(s) 277, 544, 604, 1405, 1627, 1942, 2322, 2796, 2955
BstNSI RCATGY 1 cut(s) 2169
BstSFI CTRYAG 2 cut(s) 1330, 1755
BstUI CGCG 5 cut(s) 340, 527, 825, 1305, 2223
BstV1I GCAGC 5 cut(s) 312, 1600, 2100, 2512, 2816
BstV2I GAAGAC 2 cut(s) 545, 3221
BstX2I RGATCY 2 cut(s) 864, 2587
BstXI CCANNNNNNTGG 1 cut(s) 610
BstYI RGATCY 2 cut(s) 864, 2587
BsuI GTATCC 1 cut(s) 2392
BsuRI GGCC 6 cut(s) 77, 530, 905, 1484, 2151, 2790
BtgI CCRYGG 3 cut(s) 759, 1286, 3017
BtsCI GGATG 6 cut(s) 193, 364, 431, 463, 547, 1648
BtsIMutI CAGTG 8 cut(s) 427, 835, 1424, 1741, 2275, 2537, 3011, 3027
BveI ACCTGC 1 cut(s) 1397
Cac8I GCNNGC 8 cut(s) 495, 629, 648, 652, 2227, 2346, 2523, 2736
CciI TCATGA 1 cut(s) 2158
CfoI GCGC 4 cut(s) 166, 1598, 2225, 3012
Cfr13I GGNCC 7 cut(s) 114, 600, 732, 1356, 2204, 2386, 3023
CseI GACGC 3 cut(s) 75, 335, 1311
CspCI CAANNNNNGTGG 2 cut(s) 3016, 3051
DdeI CTNAG 8 cut(s) 1011, 1479, 1812, 2053, 2274, 2691, 2816, 2910
DpnI GATC 8 cut(s) 866, 929, 1336, 1500, 1776, 2589, 3129, 3171
DpnII GATC 8 cut(s) 864, 927, 1334, 1498, 1774, 2587, 3127, 3169
EaeI YGGCCR 3 cut(s) 528, 903, 2149
Eam1104I CTCTTC 2 cut(s) 177, 1233
EarI CTCTTC 2 cut(s) 177, 1233
EciI GGCGGA 1 cut(s) 614
Eco130I CCWWGG 5 cut(s) 304, 759, 1286, 2729, 3017
Eco147I AGGCCT 1 cut(s) 2790
Eco47I GGWCC 7 cut(s) 114, 600, 732, 1356, 2204, 2386, 3023
Eco47III AGCGCT 1 cut(s) 165
Eco57I CTGAAG 5 cut(s) 732, 1400, 1424, 1584, 3209
Eco72I CACGTG 1 cut(s) 1321
Eco88I CYCGRG 1 cut(s) 638
EcoO109I RGGNCCY 1 cut(s) 3023
EcoRI GAATTC 1 cut(s) 2637
EcoRII CCWGG 9 cut(s) 275, 542, 602, 1403, 1625, 1940, 2320, 2794, 2953
EcoT14I CCWWGG 5 cut(s) 304, 759, 1286, 2729, 3017
EcoT22I ATGCAT 1 cut(s) 1997
ErhI CCWWGG 5 cut(s) 304, 759, 1286, 2729, 3017
Esp3I CGTCTC 1 cut(s) 3148
FalI AAGNNNNNCTT 4 cut(s) 1668, 1700, 2616, 2648
FaqI GGGAC 3 cut(s) 100, 387, 3009
FauNDI CATATG 1 cut(s) 1997
FblI GTMKAC 2 cut(s) 828, 2543
Fnu4HI GCNGC 7 cut(s) 222, 326, 528, 1589, 2114, 2526, 2830
FokI GGATG 6 cut(s) 180, 371, 418, 470, 534, 1655
Fsp4HI GCNGC 7 cut(s) 222, 326, 528, 1589, 2114, 2526, 2830
GlaI GCGC 4 cut(s) 165, 1597, 2224, 3011
GluI GCNGC 7 cut(s) 222, 326, 528, 1589, 2114, 2526, 2830
GsaI CCCAGC 1 cut(s) 1045
GsuI CTGGAG 3 cut(s) 259, 936, 1609
HaeII RGCGCY 2 cut(s) 167, 3013
HaeIII GGCC 6 cut(s) 77, 530, 905, 1484, 2151, 2790
HapII CCGG 3 cut(s) 112, 449, 2939
HgaI GACGC 3 cut(s) 75, 335, 1311
HhaI GCGC 4 cut(s) 166, 1598, 2225, 3012
Hin1I GRCGYC 3 cut(s) 346, 426, 2599
Hin6I GCGC 4 cut(s) 164, 1596, 2223, 3010
HinP1I GCGC 4 cut(s) 164, 1596, 2223, 3010
HincII GTYRAC 2 cut(s) 1008, 2574
HindII GTYRAC 2 cut(s) 1008, 2574
HindIII AAGCTT 1 cut(s) 2625
HpaI GTTAAC 1 cut(s) 1008
HpaII CCGG 3 cut(s) 112, 449, 2939
HphI GGTGA 5 cut(s) 320, 554, 1319, 1994, 2999
Hpy188I TCNGA 9 cut(s) 13, 34, 751, 982, 1147, 1813, 2505, 2968, 3051
Hpy99I CGWCG 3 cut(s) 23, 351, 2100
HpyCH4IV ACGT 4 cut(s) 21, 426, 1320, 2599
HpyF10VI GCNNNNNNNGC 9 cut(s) 473, 482, 643, 1244, 1398, 1527, 1671, 2714, 2796
HpyF3I CTNAG 8 cut(s) 1011, 1479, 1812, 2053, 2274, 2691, 2816, 2910
HpySE526I ACGT 4 cut(s) 21, 426, 1320, 2599
Hsp92I GRCGYC 3 cut(s) 346, 426, 2599
HspAI GCGC 4 cut(s) 164, 1596, 2223, 3010
KflI GGGWCCC 1 cut(s) 3023
KspAI GTTAAC 1 cut(s) 1008
Kzo9I GATC 8 cut(s) 864, 927, 1334, 1498, 1774, 2587, 3127, 3169
LguI GCTCTTC 1 cut(s) 1233
LmnI GCTCC 9 cut(s) 306, 643, 761, 1475, 1628, 2569, 2714, 2881, 3052
Lsp1109I GCAGC 5 cut(s) 312, 1600, 2100, 2512, 2816
MaeII ACGT 4 cut(s) 21, 426, 1320, 2599
MalI GATC 8 cut(s) 866, 929, 1336, 1500, 1776, 2589, 3129, 3171
MboI GATC 8 cut(s) 864, 927, 1334, 1498, 1774, 2587, 3127, 3169
MfeI CAATTG 1 cut(s) 1218
MflI RGATCY 2 cut(s) 864, 2587
MhlI GDGCHC 3 cut(s) 639, 2275, 2719
MlsI TGGCCA 2 cut(s) 905, 2151
MluNI TGGCCA 2 cut(s) 905, 2151
MlyI GAGTC 2 cut(s) 891, 1528
MmeI TCCRAC 3 cut(s) 57, 459, 2182
Mox20I TGGCCA 2 cut(s) 905, 2151
Mph1103I ATGCAT 1 cut(s) 1997
MroXI GAANNNNTTC 2 cut(s) 2748, 2790
MscI TGGCCA 2 cut(s) 905, 2151
MseI TTAA 9 cut(s) 861, 1001, 1007, 1542, 2646, 2760, 2903, 3107, 3253
MslI CAYNNNNRTG 2 cut(s) 1417, 1996
Msp20I TGGCCA 2 cut(s) 905, 2151
MspA1I CMGCKG 1 cut(s) 2278
MspI CCGG 3 cut(s) 112, 449, 2939
MunI CAATTG 1 cut(s) 1218
Mva1269I GAATGC 5 cut(s) 738, 1414, 1434, 2944, 3066
MvaI CCWGG 9 cut(s) 277, 544, 604, 1405, 1627, 1942, 2322, 2796, 2955
MvnI CGCG 5 cut(s) 340, 527, 825, 1305, 2223
MwoI GCNNNNNNNGC 9 cut(s) 473, 482, 643, 1244, 1398, 1527, 1671, 2714, 2796
NciI CCSGG 1 cut(s) 112
NcoI CCATGG 3 cut(s) 759, 1286, 3017
NdeI CATATG 1 cut(s) 1997
NdeII GATC 8 cut(s) 864, 927, 1334, 1498, 1774, 2587, 3127, 3169
NlaIV GGNNCC 7 cut(s) 116, 757, 765, 2490, 2800, 3024, 3025
NmuCI GTSAC 5 cut(s) 133, 422, 427, 1376, 2056
NsiI ATGCAT 1 cut(s) 1997
NspI RCATGY 1 cut(s) 2169
OliI CACNNNNGTG 1 cut(s) 1417
PaeR7I CTCGAG 1 cut(s) 638
PagI TCATGA 1 cut(s) 2158
PaqCI CACCTGC 1 cut(s) 1397
PasI CCCWGGG 1 cut(s) 1941
PceI AGGCCT 1 cut(s) 2790
PciSI GCTCTTC 1 cut(s) 1233
PctI GAATGC 5 cut(s) 738, 1414, 1434, 2944, 3066
PdmI GAANNNNTTC 2 cut(s) 2748, 2790
PfeI GAWTC 8 cut(s) 58, 280, 917, 1148, 1315, 2744, 2914, 2963
PflMI CCANNNNNTGG 2 cut(s) 912, 1048
PfoI TCCNGGA 1 cut(s) 275
PkrI GCNGC 7 cut(s) 223, 327, 529, 1590, 2115, 2527, 2831
PleI GAGTC 2 cut(s) 890, 1528
PmaCI CACGTG 1 cut(s) 1321
PmlI CACGTG 1 cut(s) 1321
PpsI GAGTC 2 cut(s) 890, 1528
Ppu21I YACGTR 1 cut(s) 1321
PpuMI RGGWCCY 1 cut(s) 3023
Psp5II RGGWCCY 1 cut(s) 3023
Psp6I CCWGG 9 cut(s) 275, 542, 602, 1403, 1625, 1940, 2320, 2794, 2953
PspCI CACGTG 1 cut(s) 1321
PspFI CCCAGC 1 cut(s) 1041
PspGI CCWGG 9 cut(s) 275, 542, 602, 1403, 1625, 1940, 2320, 2794, 2953
PspN4I GGNNCC 7 cut(s) 116, 757, 765, 2490, 2800, 3024, 3025
PspPI GGNCC 7 cut(s) 114, 600, 732, 1356, 2204, 2386, 3023
PspPPI RGGWCCY 1 cut(s) 3023
PspXI VCTCGAGB 1 cut(s) 638
PstI CTGCAG 1 cut(s) 1759
PsuI RGATCY 2 cut(s) 864, 2587
RseI CAYNNNNRTG 2 cut(s) 1417, 1996
SapI GCTCTTC 1 cut(s) 1233
SaqAI TTAA 9 cut(s) 861, 1001, 1007, 1542, 2646, 2760, 2903, 3107, 3253
SatI GCNGC 7 cut(s) 222, 326, 528, 1589, 2114, 2526, 2830
Sau3AI GATC 8 cut(s) 864, 927, 1334, 1498, 1774, 2587, 3127, 3169
Sau96I GGNCC 7 cut(s) 114, 600, 732, 1356, 2204, 2386, 3023
SchI GAGTC 2 cut(s) 891, 1528
SduI GDGCHC 3 cut(s) 639, 2275, 2719
SfcI CTRYAG 2 cut(s) 1330, 1755
Sfr274I CTCGAG 1 cut(s) 638
SinI GGWCC 7 cut(s) 114, 600, 732, 1356, 2204, 2386, 3023
SlaI CTCGAG 1 cut(s) 638
SmiMI CAYNNNNRTG 2 cut(s) 1417, 1996
SmlI CTYRAG 5 cut(s) 638, 815, 1427, 2108, 2548
SmoI CTYRAG 5 cut(s) 638, 815, 1427, 2108, 2548
SpeI ACTAGT 3 cut(s) 2086, 2446, 2840
SseBI AGGCCT 1 cut(s) 2790
SsiI CCGC 8 cut(s) 222, 328, 527, 565, 625, 1252, 1305, 2278
SspI AATATT 1 cut(s) 1490
StuI AGGCCT 1 cut(s) 2790
StyI CCWWGG 5 cut(s) 304, 759, 1286, 2729, 3017
TaiI ACGT 4 cut(s) 24, 429, 1323, 2602
TaqI TCGA 1 cut(s) 639
TaqII GACCGA 1 cut(s) 152
TatI WGTACW 5 cut(s) 507, 839, 1176, 2089, 3179
TauI GCSGC 2 cut(s) 224, 530
TfiI GAWTC 8 cut(s) 58, 280, 917, 1148, 1315, 2744, 2914, 2963
Tru1I TTAA 9 cut(s) 861, 1001, 1007, 1542, 2646, 2760, 2903, 3107, 3253
Tru9I TTAA 9 cut(s) 861, 1001, 1007, 1542, 2646, 2760, 2903, 3107, 3253
TscAI CASTG 8 cut(s) 427, 842, 1424, 1741, 2275, 2544, 3011, 3034
TseFI GTSAC 5 cut(s) 133, 422, 427, 1376, 2056
TseI GCWGC 5 cut(s) 325, 1588, 2113, 2525, 2829
Tsp45I GTSAC 5 cut(s) 133, 422, 427, 1376, 2056
TspGWI ACGGA 2 cut(s) 1221, 3172
TspRI CASTG 8 cut(s) 427, 842, 1424, 1741, 2275, 2544, 3011, 3034
Van91I CCANNNNNTGG 2 cut(s) 912, 1048
VpaK11BI GGWCC 7 cut(s) 114, 600, 732, 1356, 2204, 2386, 3023
XapI RAATTY 7 cut(s) 215, 1280, 2154, 2637, 2777, 3052, 3076
XbaI TCTAGA 1 cut(s) 2752
XceI RCATGY 1 cut(s) 2169
XhoI CTCGAG 1 cut(s) 638
XmaJI CCTAGG 1 cut(s) 2729
XmiI GTMKAC 2 cut(s) 828, 2543
XmnI GAANNNNTTC 2 cut(s) 2748, 2790
ZraI GACGTC 2 cut(s) 427, 2600
Zsp2I ATGCAT 1 cut(s) 1997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.