Prupe.1G563000_v2.0.a1

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000800
Physical Location & Seq
Reverse (-)
45990217 .. 46010193
19977 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G563000.2

Sequence Viewer

Length: 6633 bp
ATGGACGAGACCTCCATCATTTCGAAGCTTGAAACCTCCTGCTCCAGCGGCCTTCATCCCCTCGTCTCCGACTACCTTCAGCCATTTACCCAACTCCAGAATCCCAAGAAAACCAAAAAATCAACCAAATCCCAAGACCAGCAAACCCTTCTCCGCTCGCTTGCTAAGAAGTTCCTCCCCTTCATCAACCGCACGCTATCCCTTCTCCCCAAACGCCTCGCCGGTTTCTCCAAGTTAGACGACGAATTTACCCTCGAACTCTTCGATATTTACAGGCTTTGCCTCGATTGCCTGGACTCCTTGTCCTCTGTGTTGTCCGGTACGCCCTACTCGTTTAACCTCCAGAGGGTTCGGATGGTGACATGCCTCGAGGCATGTGGGCGGTACAAGGATGCAGAGAGCGAGGGGTTTAGGGTTTTGGAGAGCCTTAAGGCGATTGAATTTGGGTCGAACACACGGGTCAAATCGGACCGAAGATTTGTGCCAGACGTGGAGAAGGGGAGCGGTGATAAGGATTTGGGGTCTTTGGTAGGAGGAATTGTGGTGACGCTTGTGAGATGTGCGGGCATGAATCAAAGTAAGGATAGTGAGGTGTATAGGAGGGTGCTTTGTTTGGTTGAGGAGGTCATGCCGTGGTTCAGGGTATTAGACGCAAACACATATGAGAAGTTGCACAGGATGCTTGTGTCCTCTCTGAGTAAATGTACTCAATTTTTAGTTGGGGAGCTATCATCGTTTGAGGGAGATCTTGTGAAAATGTTCTGCCTTGTAACCATGACTGAGTATGCCAAGTCATCAATGAAAGATCAAATTTTCAAGTTTGCTCATCGGATCTGTTCATCTTTATTTTTGTTTCAAAAGGATAGATGCTTGCTCATTGACATATTATTTTGCTTGTTGGACTCCCTTGTTCGCGAATGTAAGGTTGAAGTGGAAAATACAGGAAAAGAGTTTGTTGAACTTATTGCTTATTGTGCCAAGAAATGTCGAACTACAAATACAAATCTTTGCAGTATTATTGGGTCCCATTTAAATGAATTAGCGGGTGATTTCCATCAGGTTAGAACACCTTTTCATTTGATTCTGAGGCTTTATGCCTCTGGATTGCACTTTTTTGATCGCAGTATGAAGTCAAAAGCTGGTGGAGGTGCAATTAGAATTTTGCATGATGATGGGGATGCAATGAATCGCTTGTCTGATTTGCTTGGTTTGTTGAGAAGTTATTTCCAAATTGGCCACAATGAAGACACTGTATTATCCAATTTGCAGTTGATTTCGAAGAGTGCAGCCTCCATGAGTCAACTTCAGAGGGATAGAAAGGACTATATTCTGTCTTACTTTAACGCATTGAAATTTTTATGCCAGCCACTTACGGAGTTAGTAAATTCAGGAAAGAAAGAGATTCTTACTGATAACGAAGCTGCTTCTGTTTCTACTGAACTGTGTGATATCCAGGGTGCTTTCCATCAGTTTTATGATGTTTTCGTTTTTTTTCAAACGTGCAGATGTACATATGAGGTGGATAGAGATGTATTTGACGACAGAGATATATTTGATGACAACAGCATCATTAGTGTTGCTTTGGCTTCTTTTACCCTCTCCATCAGAACAAAGCTTAACATACAGAAGAGTGTGCAAATACTTGAAAATGTCATCACCAGTGATTGGATTCAACCTAATGGTCTGAAGCATCTATATGTCTCTCTCTACAATACTGGTGTACTTTTCTACCGAAACAAGGAATTAAAAGAGGCATCAGAGGCATTGAAGTTTTGTTGTAAAGCATCATGGACTTGTGTTATATGTGTTTGTGATATGTTTGTACACAAAGTAAAGGTGCCCCAGGTTGATCTGTCGGAAGATGCTATTGTAGATTTCTTTGATGAGTGCTGTAAGAGAAGTGCCTTTCTTTTGGATGTTCTTAATCAATTGCAAAGTCACGATGTGAAAAGAACTATATTAGAGAGCTTTGAAAATTGGTCTATTGCTGCAAATTTGTTTCAGAGGCTGCCAGGTCCGTTGTCTTTGGTGAAGCAGTGGGTTAAGATGGAATGTAAACATTACAAGAATGTGGATGTTGAGGATGATGCTCCTACCTTATACAGTTTGCTATCATCTTCTAAGAAAGTAACAAAGAAGACAATCGAAATTGTCTTGGAGCAGGAACTTCTTGCTTATGAGGAAATGAATGATGTGAACCCTGAGTTTTGTCAGAAAATGCAAATGAAAATTATAGATATCCTACTGCAATACGTGCATGTCACACCAGATAGTTGCTTACAAAAATCAAGAATTTTATTGAGAAAGGGAAGAGCATTAAGGCTTTCTGGCATCAGCGGTCTGAAGGGCTGTATTCAGTGTTTATCAGATGCAATATCTTCACTTAATGAGATGTATGATGAAACCTATATCCATGAAATTTCTCCTTGCCATCAATTAGCTGTGGCATATTGCTTACGCGCACTTTGTACCCAAGAAGCTGAACCCAACTCGAAGCAAGTGCTTGAAGATATCAGTTCTGCCATTAATTTATGGTTGGGCATTTCTACTCGAAATAATTGTTCTCCAGATGACAAGTGCTCTATGGTGTCAGAAAGTATTATGTTGCTGCTTTACAATGCTATTGATTTGTTATCCATCAAGGGTTGCATGGACTTTCACAATGATATACATAGGCTTATGATTAGATTATTTAAATGGAGGGATGTCCCATTAGAGAAGTGTGTGGCCAGATTGTGGGAATGTAGAAGGATTAGCCATGGCCTTTGTGCTTCACCTGTGAATGAGGCATTCATCATGAACTTATCAGATCATTGTGGTGAAAATTCAAAGTCTATTGAGTTTTGGGTAGATTGCCTGAAAGAATCCAAACCACTTCTATTAGCGTTCCAATATAATTTATCATCTGTGTCTCCAAACTTTCCTCGGGGCTCATGCAATTATGAAAGCTCATTTCGATCAGATATCACAATTGATGAAGTTAAGGAAGCTGCTTTTGAACTTATTTCAAGTGTTCCTGTATTGAGTTCGTCTGCCTACATAGCTGGATATCTCTACTATGATTTATGCGAAAGACTTGTTTCAAATGGAAGGTTAATTGAGGCTCTGTCATATGCAAAGGAAGCTTACCAGTTGCGTGCTAAACTATTTAGAGAGAAATTTATGTACTCTTCTGAACAGTCCAAAACGTGCAATGAAGCTGGGGGTAGCGGTGAGAAATTAACATATCATATTCAGGATATGCATATGCACATATCAGTTGCTAGTGAATTTTGGTCCTTTGATGCTAGCCTATGTGACTTGGAACGTTGTTATCTGAGTCCATGGAATGTGCTTCAATGTTATCTTGAAAGCACTCTTCAGATAGGCGTTATCCATGAAATAATTGGAAACAGAGCTGGGGCTGAAGGTTTTCTACAATTGGGAAAAGCTATTTCCTGCTCACAAAGCTTGCCACTATTTATAATTGTTTTTTCTACTGTATTGGGAAAGCTGTACCACAAGCAGCAGCTTTGGGATTTTGCAGAAAAGGAACTTCAAAGTGCGAAGCAATATTTGCGGGCTAGTAGCACAGATATTTCTTGCTTGAAGTGCAGATTAATGCTGGAAGCAACTGTGAATCAGAATCTTGGTGATTTATTCCAAAGCATGTTTTATAACACAAGGAATACATCTTTAGATAAATTATCTCTTGCTGAAAACCTGTACAAATCGGCAATAGCGAAGCTTAATCTTTCTGAGTGGAAAAATTCGGTTAGTTGTCCTGAACAAGGCTGGGTTGAAAGCACAAGGCTTCGAAAAACTATTCTTAAAGATGTTGGATCTTGTGCTAGCAGTACATTTACACATTCTGAAGAAAATCAGGAGGATATTGGGAAACCCACAAGGGAGGGGCTGAAAGGCAAGAAGGAAGTTAAAAAGTGTAAAAAGACTAACAATGCACCAAAACCGGTGGTGAAGGATCAAGATGCAATACCTGAGTATAATTTGAGGTCAACTCGGTCAAGATATCAATCTTCTCAGAACCAAAGTATAAGTGGTAATGGCGTTGTCCAAGTTGGTCATTCAAAACAATTGAAAGGCAATAGCAAGTCTGATTGTCCTGATACTTTTAGGAAGAGGGAATTTCTTTTGGATTTAAAAAGTTGTGAGGTTGCTTTTGGGTGTGATGTAACATGCATTTGCAACAAAATGAGTTGTTGGCAGTGTCTTCCTGTGGAAGTTTTGGATTCTGGGTTAGTTAAAAATTTAGTTGATTTGAAGTGGGAGTTTGTCCGCAGGCGCCTGTCACTCAGGCTTCTCACAGGATTAGGAAAATGCTTGGAGAGTCGTGGTCAGATTCAAGAAACACATGAAATTACGTTGCAAACCGTATCAATCTTGGTCAGCCGAAACGCATTTTGTCTTATTACCTCATCTGTTTCATCGACATCCTTTCTCAATTTAATGGGAAAGGAGATCCCAGGGGATGTCTTCAGTGTTGAACGGGCAGAAGTACTGTTGAATATAAGTTGGTTGTCTTTGAAGAGTTACTGTTCTAAGGAAACCAGGATTATTTGCTCCGATCTGCCTCGTATTGAGCTACCGAAACTAGTGTCTTGGTTGATGCTAGCCTTTGTTCTCTGCCGTGACGTTCCTGTACTTTTTCAGAAGGTTTCCAGATTGCTTGCTGCTATATTTTTACTTTCTGCCTCTAGTGAGCGTTTTTCTTTGTCATCTTCTTCGAAAACTCTCTGCGAAAACCATTGGGCTTCTTATTTTCATCAAGCTTCACTTGGCACTCATCTTAGTTACCAATTTTTCACAAATGTATCTGACATATGTAACGTTCAACACCTTGTAAATGCTGAGGTTACTGGCTCAACTTGCATGGGATCAGGAAAAAAAAAATTACTCAGGCTTGCACCTGAGTCTATTCAAGAACTTGAAGAGTTTGTAACAATATTTTTTGCCGGCCTTCCTTGCACAACAATCATCTGTATAAGTTTGCTTGCAGGCCCTTATGTTAGTTTGTTAGAAGAACTATTCCCCGTTCCTTCTTGTGTTCATGCATGGATTCTGGTATCACGCCTGAACTCTGAGAGTCAACCTATCGTTATGCTTCTGCCAGTGGATTCAGTATTAGAAGGTTCTGCAGAAGATTCAGATGATGTTGCGAATTCTGGTTCTGGTAGTTTTTCTGAAAGAAAGGATACTGGAAAGCGTTGGCACTGTCCATGGGGTTTCACTGTGGTTGACAAGGTAGCTCCAGAATTTAGGTTGATATTGGAGGAGAGTTTTTCGTCAGCTTCATTAATTTTTGAAGAAGATACAAAAAACGCTTGGACATTCTGGTGGATGTGGAGACAGAAGCTTGATCTTCGCCTTGGTAAATTGCTGAAGAACTTAGAAGATTCATGGTTTGGTCCTTGGAGGTGTGTGCTTCTGGGAGAATGGTCAAACTGCAAGCAGCTGGACTTGGTACATAAGAAGCTGGTGCGGGATTTGAAATCTAAGTGCAAAGTGGACATAGATGAGAGTCTTCTCAAAGTCATTCTTGGGGGTTCCAAATGTGCCTTTGAAGGAGGTGCATATGTTTCACAGCTCTGTTTTAAGAAAGGTTGCTATATTGGTAAAGCTGGATGTTCTGGGGAAGAGAAGTGTTTGACATCACCCGATGAATCTAATGGTATTGAGAAAGAGTCTGAGTTGGCCTTCCAACTAATACATGAAGCAGTAAATGAGCTTGAAGGGCTATGTTCTGTAAATAGAGAGCCAATAATTCTAGTGCTGGACTTCGAGGTTCAGATGCTTCCTTGGGAGAATTTACCAATACTAAGAAACAAGGAGGTTTATCGCATGCCTTCTATTGGGAGCATCTCTTCAACACTGGAGAAGAACTACCACCATCAAGATCAAGTCGCGAATAATATTACTGCATTCCCTTTGATAGATCCATTGGATTCATTTTATCTCCTGAACCCCAGTGGTGATCTAGGTACCACGCAAATCGAATTTGAGAAGTGGTTTAGAGATCAAAATTTGGAGGGGAAAGCTGGATGTGCACCCCCAGCTGAAGAATTGGCAGTGGCCTTGAAAAGCCATGATCTCTTTATATATTTTGGCCATGGAAGTGGGGTACAATATATCCCTATGCATCAAATTCAGAGACTGGAAAACTGTGCCGCCACTCTCCTGATGGGATGTAGTAGTGGTTCCCTGAAACTGAATGGGTGTTATATTCCACATGGTCCCCCACTGTCTTATCTGCTAGCTGGTTCTCCTGTCATTGTTGCAAACTTATGGGAAGTGACGGATAAGGATATTAACCGATTTGCGAAGGCCATGCTTGACGGTTGGCTAAAAGAACGATGGAGTCCCTCTGAGGGCTGTGTCCAATGCAAAGTAGCAGAAGAATTTGAAGCCATGAGCATTAGGGGCCGTAAAGGTAATGCCAAGAAGAAAATATCAAAGAAGAAATTGCCTGAAGCTTGCGAGAGCAGCGATCCCCCAATTAAGATTTCTTGTGATCATAAGCCGAAGATTGGATCATTCATGAGTCAAGCTCGCGAAGCTTGCAGTCTTCCTTTCTTGACTGGAGCATCACCAGTATGTTATGGTGTTCCAACAGGCATAAGGAAAAAAGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

2211

Amino Acids

249.1

Weight (kDa)

6.47

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3464, 3657
AbsI CCTCGAGG 1 cut(s) 368
Acc65I GGTACC 1 cut(s) 5978
AccB1I GGYRCC 3 cut(s) 1837, 4281, 5978
AccB7I CCANNNNNTGG 2 cut(s) 1665, 2736
AccBSI CCGCTC 2 cut(s) 156, 504
AccII CGCG 4 cut(s) 915, 2460, 5903, 6549
AclI AACGTT 2 cut(s) 3307, 4827
AclWI GGATC 8 cut(s) 839, 3829, 3969, 4453, 4882, 5927, 6479, 6535
AcoI YGGCCR 3 cut(s) 1234, 2727, 6103
AcyI GRCGYC 1 cut(s) 4282
AdeI CACNNNGTG 1 cut(s) 1945
AfiI CCNNNNNNNGG 8 cut(s) 346, 1665, 1738, 2736, 3770, 5849, 6365, 6524
AflII CTTAAG 1 cut(s) 428
AgeI ACCGGT 1 cut(s) 3949
AhdI GACNNNNNGTC 1 cut(s) 301
AhlI ACTAGT 1 cut(s) 4591
AjiI CACGTC 1 cut(s) 490
AjnI CCWGG 6 cut(s) 291, 1452, 1842, 2011, 4462, 4547
AjuI GAANNNNNNNTTGG 2 cut(s) 6484, 6516
Alw21I GWGCWC 2 cut(s) 2582, 6046
Alw26I GTCTC 6 cut(s) 2, 70, 1705, 2916, 5338, 6142
Alw44I GTGCAC 1 cut(s) 6042
AlwI GGATC 8 cut(s) 839, 3829, 3969, 4453, 4882, 5927, 6479, 6535
AlwNI CAGNNNCTG 2 cut(s) 2008, 6151
Ama87I CYCGRG 2 cut(s) 368, 2925
ApaLI GTGCAC 1 cut(s) 6042
ArsI GACNNNNNNTTYG 4 cut(s) 461, 493, 4580, 4612
AseI ATTAAT 3 cut(s) 2526, 3599, 5294
AsiGI ACCGGT 1 cut(s) 3949
Asp700I GAANNNNTTC 2 cut(s) 758, 3411
Asp718I GGTACC 1 cut(s) 5978
AspLEI GCGC 2 cut(s) 2462, 4284
AspS9I GGNCC 8 cut(s) 469, 1023, 2015, 3276, 4997, 5405, 6230, 6418
AsuII TTCGAA 4 cut(s) 23, 1277, 3796, 4724
AsuNHI GCTAGC 4 cut(s) 3287, 3830, 4609, 6250
AvaI CYCGRG 2 cut(s) 368, 2925
AvaII GGWCC 6 cut(s) 469, 1023, 2015, 3276, 5405, 6230
BaeGI GKGCMC 2 cut(s) 1842, 6046
BaeI ACNNNNGTAYC 2 cut(s) 4794, 4827
BalI TGGCCA 3 cut(s) 1236, 2729, 6105
BanI GGYRCC 3 cut(s) 1837, 4281, 5978
BanII GRGCYC 1 cut(s) 2933
BarI GAAGNNNNNNTAC 2 cut(s) 3399, 3431
BbsI GAAGAC 6 cut(s) 1251, 2144, 4202, 4465, 5513, 6554
Bbv12I GWGCWC 2 cut(s) 2582, 6046
BbvCI CCTCAGC 1 cut(s) 4848
BceAI ACGGC 3 cut(s) 616, 4611, 6405
BcgI CGANNNNNNTGC 4 cut(s) 2482, 2516, 6307, 6341
BciT130I CCWGG 6 cut(s) 293, 1454, 1844, 2013, 4464, 4549
BciVI GTATCC 1 cut(s) 5185
BclI TGATCA 1 cut(s) 6508
BcoDI GTCTC 6 cut(s) 2, 70, 1705, 2916, 5338, 6142
BcuI ACTAGT 1 cut(s) 4591
BfmI CTRYAG 1 cut(s) 5133
BfoI RGCGCY 1 cut(s) 4285
BfrI CTTAAG 1 cut(s) 428
BfuI GTATCC 1 cut(s) 5185
BglII AGATCT 1 cut(s) 745
BmcAI AGTACT 1 cut(s) 4497
Bme1390I CCNGG 6 cut(s) 293, 1454, 1844, 2013, 4464, 4549
Bme18I GGWCC 6 cut(s) 469, 1023, 2015, 3276, 5405, 6230
BmeRI GACNNNNNGTC 1 cut(s) 301
BmeT110I CYCGRG 2 cut(s) 368, 2925
BmgBI CACGTC 1 cut(s) 490
BmgT120I GGNCC 8 cut(s) 469, 1023, 2015, 3276, 4997, 5405, 6230, 6418
BmiI GGNNCC 9 cut(s) 1024, 1025, 1839, 4283, 5545, 5980, 6196, 6232, 6419
BmrFI CCNGG 6 cut(s) 293, 1454, 1844, 2013, 4464, 4549
BmrI ACTGGG 1 cut(s) 5958
BmtI GCTAGC 4 cut(s) 3291, 3834, 4613, 6254
BmuI ACTGGG 1 cut(s) 5958
BpiI GAAGAC 6 cut(s) 1251, 2144, 4202, 4465, 5513, 6554
BplI GAGNNNNNCTC 4 cut(s) 3982, 4014, 6529, 6561
BpmI CTGGAG 7 cut(s) 28, 80, 326, 2550, 5232, 5891, 6597
Bpu10I CCTNAGC 1 cut(s) 4848
Bpu14I TTCGAA 4 cut(s) 23, 1277, 3796, 4724
BsaAI YACGTR 1 cut(s) 2254
BsaBI GATNNNNATC 2 cut(s) 1053, 4012
BsaHI GRCGYC 1 cut(s) 4282
BsaI GGTCTC 1 cut(s) 2
BsaWI WCCGGW 2 cut(s) 317, 3949
BsaXI ACNNNNNCTCC 5 cut(s) 26, 525, 555, 735, 765
Bsc4I CCNNNNNNNGG 8 cut(s) 346, 1665, 1738, 2736, 3770, 5849, 6365, 6524
Bse118I RCCGGY 3 cut(s) 221, 3949, 4952
Bse3DI GCAATG 2 cut(s) 1188, 3199
Bse8I GATNNNNATC 2 cut(s) 1053, 4012
BseBI CCWGG 6 cut(s) 293, 1454, 1844, 2013, 4464, 4549
BseJI GATNNNNATC 2 cut(s) 1053, 4012
BseLI CCNNNNNNNGG 8 cut(s) 346, 1665, 1738, 2736, 3770, 5849, 6365, 6524
BseMI GCAATG 2 cut(s) 1188, 3199
BseRI GAGGAG 2 cut(s) 635, 5285
BseSI GKGCMC 2 cut(s) 1842, 6046
BseYI CCCAGC 4 cut(s) 3200, 3398, 3774, 6049
BsgI GTGCAG 3 cut(s) 1305, 1522, 3613
Bsh1236I CGCG 4 cut(s) 915, 2460, 5903, 6549
BshNI GGYRCC 3 cut(s) 1837, 4281, 5978
BshTI ACCGGT 1 cut(s) 3949
BsiHKAI GWGCWC 2 cut(s) 2582, 6046
BsiHKCI CYCGRG 2 cut(s) 368, 2925
BsiSI CCGG 4 cut(s) 222, 318, 3950, 4953
BslFI GGGAC 4 cut(s) 1009, 2693, 6216, 6342
BslI CCNNNNNNNGG 8 cut(s) 346, 1665, 1738, 2736, 3770, 5849, 6365, 6524
BsmAI GTCTC 6 cut(s) 2, 70, 1705, 2916, 5338, 6142
BsmBI CGTCTC 1 cut(s) 70
BsmFI GGGAC 4 cut(s) 1009, 2693, 6216, 6342
BsmI GAATGC 2 cut(s) 2789, 5918
Bso31I GGTCTC 1 cut(s) 2
BsoBI CYCGRG 2 cut(s) 368, 2925
Bsp119I TTCGAA 4 cut(s) 23, 1277, 3796, 4724
Bsp1286I GDGCHC 4 cut(s) 1842, 2582, 2933, 6046
Bsp1407I TGTACA 3 cut(s) 1508, 1822, 3705
Bsp19I CCATGG 4 cut(s) 2758, 3323, 5216, 6106
Bsp68I TCGCGA 3 cut(s) 915, 5903, 6549
BspFNI CGCG 4 cut(s) 915, 2460, 5903, 6549
BspHI TCATGA 2 cut(s) 2796, 6534
BspLI GGNNCC 9 cut(s) 1024, 1025, 1839, 4283, 5545, 5980, 6196, 6232, 6419
BspMAI CTGCAG 1 cut(s) 5137
BspOI GCTAGC 4 cut(s) 3291, 3834, 4613, 6254
BspPI GGATC 8 cut(s) 839, 3829, 3969, 4453, 4882, 5927, 6479, 6535
BspQI GCTCTTC 1 cut(s) 2305
BspT104I TTCGAA 4 cut(s) 23, 1277, 3796, 4724
BspT107I GGYRCC 3 cut(s) 1837, 4281, 5978
BspTI CTTAAG 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 2
BsrBI CCGCTC 2 cut(s) 156, 504
BsrDI GCAATG 2 cut(s) 1188, 3199
BsrFI RCCGGY 3 cut(s) 221, 3949, 4952
BsrGI TGTACA 3 cut(s) 1508, 1822, 3705
BssAI RCCGGY 3 cut(s) 221, 3949, 4952
BssNI GRCGYC 1 cut(s) 4282
BssT1I CCWWGG 7 cut(s) 2758, 3323, 5216, 5365, 5408, 5795, 6106
Bst2UI CCWGG 6 cut(s) 293, 1454, 1844, 2013, 4464, 4549
BstACI GRCGYC 1 cut(s) 4282
BstAFI CTTAAG 1 cut(s) 428
BstAPI GCANNNNNTGC 3 cut(s) 679, 2254, 3556
BstAUI TGTACA 3 cut(s) 1508, 1822, 3705
BstBAI YACGTR 1 cut(s) 2254
BstBI TTCGAA 4 cut(s) 23, 1277, 3796, 4724
BstDSI CCRYGG 5 cut(s) 632, 2758, 3323, 5216, 6106
BstENI CCTNNNNNAGG 1 cut(s) 3768
BstFNI CGCG 4 cut(s) 915, 2460, 5903, 6549
BstH2I RGCGCY 1 cut(s) 4285
BstHHI GCGC 2 cut(s) 2462, 4284
BstMAI GTCTC 6 cut(s) 2, 70, 1705, 2916, 5338, 6142
BstNI CCWGG 6 cut(s) 293, 1454, 1844, 2013, 4464, 4549
BstNSI RCATGY 6 cut(s) 366, 378, 2261, 3652, 4179, 5842
BstSCI CCNGG 6 cut(s) 291, 1452, 1842, 2011, 4462, 4547
BstSFI CTRYAG 1 cut(s) 5133
BstSLI GKGCMC 2 cut(s) 1842, 6046
BstUI CGCG 4 cut(s) 915, 2460, 5903, 6549
BstV2I GAAGAC 6 cut(s) 1251, 2144, 4202, 4465, 5513, 6554
BstX2I RGATCY 5 cut(s) 745, 831, 3821, 4458, 5932
BstXI CCANNNNNNTGG 1 cut(s) 6113
BstYI RGATCY 5 cut(s) 745, 831, 3821, 4458, 5932
BsuI GTATCC 1 cut(s) 5185
BtgI CCRYGG 5 cut(s) 632, 2758, 3323, 5216, 6106
BtrI CACGTC 1 cut(s) 490
BtsI GCAGTG 3 cut(s) 2042, 4211, 6072
BtuMI TCGCGA 3 cut(s) 915, 5903, 6549
CaiI CAGNNNCTG 2 cut(s) 2008, 6151
CciI TCATGA 2 cut(s) 2796, 6534
CfoI GCGC 2 cut(s) 2462, 4284
Cfr10I RCCGGY 3 cut(s) 221, 3949, 4952
Cfr13I GGNCC 8 cut(s) 469, 1023, 2015, 3276, 4997, 5405, 6230, 6418
CpoI CGGWCCG 1 cut(s) 469
CseI GACGC 2 cut(s) 556, 659
CspAI ACCGGT 1 cut(s) 3949
CspCI CAANNNNNGTGG 2 cut(s) 3933, 3968
CspI CGGWCCG 1 cut(s) 469
DinI GGCGCC 1 cut(s) 4283
DraI TTTAAA 3 cut(s) 1032, 2695, 4140
DraIII CACNNNGTG 1 cut(s) 1945
DriI GACNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 3 cut(s) 1234, 2727, 6103
Eam1105I GACNNNNNGTC 1 cut(s) 301
Eco130I CCWWGG 7 cut(s) 2758, 3323, 5216, 5365, 5408, 5795, 6106
Eco24I GRGCYC 1 cut(s) 2933
Eco31I GGTCTC 1 cut(s) 2
Eco32I GATATC 6 cut(s) 1450, 2239, 2512, 2965, 3050, 4010
Eco47I GGWCC 6 cut(s) 469, 1023, 2015, 3276, 5405, 6230
Eco88I CYCGRG 2 cut(s) 368, 2925
EcoNI CCTNNNNNAGG 1 cut(s) 3768
EcoO109I RGGNCCY 2 cut(s) 1023, 4997
EcoRI GAATTC 1 cut(s) 5158
EcoRII CCWGG 6 cut(s) 291, 1452, 1842, 2011, 4462, 4547
EcoRV GATATC 6 cut(s) 1450, 2239, 2512, 2965, 3050, 4010
EcoT14I CCWWGG 7 cut(s) 2758, 3323, 5216, 5365, 5408, 5795, 6106
EcoT22I ATGCAT 4 cut(s) 3246, 4181, 5053, 6138
EcoT38I GRGCYC 1 cut(s) 2933
EgeI GGCGCC 1 cut(s) 4283
EheI GGCGCC 1 cut(s) 4283
ErhI CCWWGG 7 cut(s) 2758, 3323, 5216, 5365, 5408, 5795, 6106
Esp3I CGTCTC 1 cut(s) 70
FalI AAGNNNNNCTT 6 cut(s) 1389, 1421, 4758, 4790, 5520, 5552
FaqI GGGAC 4 cut(s) 1009, 2693, 6216, 6342
FauI CCCGC 4 cut(s) 556, 1036, 3552, 5472
FauNDI CATATG 6 cut(s) 661, 1513, 3112, 3246, 4820, 5572
FbaI TGATCA 1 cut(s) 6508
FriOI GRGCYC 1 cut(s) 2933
GlaI GCGC 2 cut(s) 2461, 4283
GsaI CCCAGC 4 cut(s) 3204, 3402, 3778, 6053
GsuI CTGGAG 7 cut(s) 28, 80, 326, 2550, 5232, 5891, 6597
HaeII RGCGCY 1 cut(s) 4285
HapII CCGG 4 cut(s) 222, 318, 3950, 4953
HgaI GACGC 2 cut(s) 556, 659
HhaI GCGC 2 cut(s) 2462, 4284
Hin1I GRCGYC 1 cut(s) 4282
Hin6I GCGC 2 cut(s) 2460, 4282
HinP1I GCGC 2 cut(s) 2460, 4282
HincII GTYRAC 4 cut(s) 1301, 3996, 5087, 5236
HindII GTYRAC 4 cut(s) 1301, 3996, 5087, 5236
HindIII AAGCTT 9 cut(s) 26, 1613, 3123, 3448, 3725, 4767, 5351, 6468, 6552
HpaII CCGG 4 cut(s) 222, 318, 3950, 4953
Hpy99I CGWCG 1 cut(s) 245
HpyCH4IV ACGT 8 cut(s) 489, 1499, 2253, 3188, 3307, 4361, 4632, 4827
HpySE526I ACGT 8 cut(s) 489, 1499, 2253, 3188, 3307, 4361, 4632, 4827
Hsp92I GRCGYC 1 cut(s) 4282
HspAI GCGC 2 cut(s) 2460, 4282
KasI GGCGCC 1 cut(s) 4281
KflI GGGWCCC 1 cut(s) 1023
KpnI GGTACC 1 cut(s) 5982
KroI GCCGGC 1 cut(s) 4952
KroNI GCCGGC 1 cut(s) 4954
Ksp22I TGATCA 1 cut(s) 6508
LguI GCTCTTC 1 cut(s) 2305
LmnI GCTCC 9 cut(s) 47, 501, 724, 2095, 2158, 4565, 5251, 5853, 6578
MaeII ACGT 8 cut(s) 489, 1499, 2253, 3188, 3307, 4361, 4632, 4827
MbiI CCGCTC 2 cut(s) 156, 504
MfeI CAATTG 4 cut(s) 1928, 2970, 3419, 4073
MflI RGATCY 5 cut(s) 745, 831, 3821, 4458, 5932
MhlI GDGCHC 4 cut(s) 1842, 2582, 2933, 6046
MlsI TGGCCA 3 cut(s) 1236, 2729, 6105
MluNI TGGCCA 3 cut(s) 1236, 2729, 6105
Mly113I GGCGCC 1 cut(s) 4282
MmeI TCCRAC 6 cut(s) 93, 879, 1836, 3799, 5722, 6629
Mox20I TGGCCA 3 cut(s) 1236, 2729, 6105
Mph1103I ATGCAT 4 cut(s) 3246, 4181, 5053, 6138
MroNI GCCGGC 1 cut(s) 4952
MroXI GAANNNNTTC 2 cut(s) 758, 3411
MscI TGGCCA 3 cut(s) 1236, 2729, 6105
MslI CAYNNNNRTG 7 cut(s) 1032, 1170, 1695, 2817, 5332, 6111, 6589
Msp20I TGGCCA 3 cut(s) 1236, 2729, 6105
MspA1I CMGCKG 4 cut(s) 48, 2337, 5452, 6053
MspCI CTTAAG 1 cut(s) 428
MspI CCGG 4 cut(s) 222, 318, 3950, 4953
MspR9I CCNGG 6 cut(s) 293, 1454, 1844, 2013, 4464, 4549
MunI CAATTG 4 cut(s) 1928, 2970, 3419, 4073
Mva1269I GAATGC 2 cut(s) 2789, 5918
MvaI CCWGG 6 cut(s) 293, 1454, 1844, 2013, 4464, 4549
MvnI CGCG 4 cut(s) 915, 2460, 5903, 6549
NaeI GCCGGC 1 cut(s) 4954
NarI GGCGCC 1 cut(s) 4282
NcoI CCATGG 4 cut(s) 2758, 3323, 5216, 6106
NdeI CATATG 6 cut(s) 661, 1513, 3112, 3246, 4820, 5572
NgoMIV GCCGGC 1 cut(s) 4952
NheI GCTAGC 4 cut(s) 3287, 3830, 4609, 6250
NlaIV GGNNCC 9 cut(s) 1024, 1025, 1839, 4283, 5545, 5980, 6196, 6232, 6419
NmuCI GTSAC 8 cut(s) 358, 544, 1937, 2260, 3296, 4287, 4628, 6289
NruI TCGCGA 3 cut(s) 915, 5903, 6549
NsiI ATGCAT 4 cut(s) 3246, 4181, 5053, 6138
NspI RCATGY 6 cut(s) 366, 378, 2261, 3652, 4179, 5842
NspV TTCGAA 4 cut(s) 23, 1277, 3796, 4724
PaeI GCATGC 1 cut(s) 5842
PaeR7I CTCGAG 1 cut(s) 368
PagI TCATGA 2 cut(s) 2796, 6534
PasI CCCWGGG 1 cut(s) 4463
PciSI GCTCTTC 1 cut(s) 2305
PcsI WCGNNNNNNNCGW 3 cut(s) 261, 329, 3719
PctI GAATGC 2 cut(s) 2789, 5918
PdiI GCCGGC 1 cut(s) 4954
PdmI GAANNNNTTC 2 cut(s) 758, 3411
PflMI CCANNNNNTGG 2 cut(s) 1665, 2736
PinAI ACCGGT 1 cut(s) 3949
PluTI GGCGCC 1 cut(s) 4285
Ppu21I YACGTR 1 cut(s) 2254
PpuMI RGGWCCY 1 cut(s) 1023
PshBI ATTAAT 3 cut(s) 2526, 3599, 5294
PsiI TTATAA 2 cut(s) 3464, 3657
Psp1406I AACGTT 2 cut(s) 3307, 4827
Psp5II RGGWCCY 1 cut(s) 1023
Psp6I CCWGG 6 cut(s) 291, 1452, 1842, 2011, 4462, 4547
PspFI CCCAGC 4 cut(s) 3200, 3398, 3774, 6049
PspGI CCWGG 6 cut(s) 291, 1452, 1842, 2011, 4462, 4547
PspN4I GGNNCC 9 cut(s) 1024, 1025, 1839, 4283, 5545, 5980, 6196, 6232, 6419
PspPI GGNCC 8 cut(s) 469, 1023, 2015, 3276, 4997, 5405, 6230, 6418
PspPPI RGGWCCY 1 cut(s) 1023
PspXI VCTCGAGB 1 cut(s) 368
PsrI GAACNNNNNNTAC 4 cut(s) 982, 1014, 6178, 6210
PstI CTGCAG 1 cut(s) 5137
PstNI CAGNNNCTG 2 cut(s) 2008, 6151
PsuI RGATCY 5 cut(s) 745, 831, 3821, 4458, 5932
PvuII CAGCTG 2 cut(s) 5452, 6053
RruI TCGCGA 3 cut(s) 915, 5903, 6549
RseI CAYNNNNRTG 7 cut(s) 1032, 1170, 1695, 2817, 5332, 6111, 6589
Rsr2I CGGWCCG 1 cut(s) 469
RsrII CGGWCCG 1 cut(s) 469
SapI GCTCTTC 1 cut(s) 2305
Sau96I GGNCC 8 cut(s) 469, 1023, 2015, 3276, 4997, 5405, 6230, 6418
ScaI AGTACT 1 cut(s) 4497
ScrFI CCNGG 6 cut(s) 293, 1454, 1844, 2013, 4464, 4549
SduI GDGCHC 4 cut(s) 1842, 2582, 2933, 6046
SfcI CTRYAG 1 cut(s) 5133
SfoI GGCGCC 1 cut(s) 4283
Sfr274I CTCGAG 1 cut(s) 368
SfuI TTCGAA 4 cut(s) 23, 1277, 3796, 4724
SinI GGWCC 6 cut(s) 469, 1023, 2015, 3276, 5405, 6230
SlaI CTCGAG 1 cut(s) 368
SmiI ATTTAAAT 2 cut(s) 1032, 2695
SmiMI CAYNNNNRTG 7 cut(s) 1032, 1170, 1695, 2817, 5332, 6111, 6589
SmlI CTYRAG 2 cut(s) 368, 428
SmoI CTYRAG 2 cut(s) 368, 428
SpeI ACTAGT 1 cut(s) 4591
SphI GCATGC 1 cut(s) 5842
SspDI GGCGCC 1 cut(s) 4281
SspI AATATT 3 cut(s) 3554, 4944, 5911
StyD4I CCNGG 6 cut(s) 291, 1452, 1842, 2011, 4462, 4547
StyI CCWWGG 7 cut(s) 2758, 3323, 5216, 5365, 5408, 5795, 6106
SwaI ATTTAAAT 2 cut(s) 1032, 2695
TaiI ACGT 8 cut(s) 492, 1502, 2256, 3191, 3310, 4364, 4635, 4830
TaqII GACCGA 2 cut(s) 486, 3990
TatI WGTACW 9 cut(s) 704, 1508, 1720, 1822, 3165, 3705, 3836, 4495, 4639
TauI GCSGC 2 cut(s) 51, 6167
TseFI GTSAC 8 cut(s) 358, 544, 1937, 2260, 3296, 4287, 4628, 6289
Tsp45I GTSAC 8 cut(s) 358, 544, 1937, 2260, 3296, 4287, 4628, 6289
TspGWI ACGGA 3 cut(s) 1388, 2007, 6308
Van91I CCANNNNNTGG 2 cut(s) 1665, 2736
Vha464I CTTAAG 1 cut(s) 428
VneI GTGCAC 1 cut(s) 6042
VpaK11BI GGWCC 6 cut(s) 469, 1023, 2015, 3276, 5405, 6230
VspI ATTAAT 3 cut(s) 2526, 3599, 5294
XagI CCTNNNNNAGG 1 cut(s) 3768
XceI RCATGY 6 cut(s) 366, 378, 2261, 3652, 4179, 5842
XcmI CCANNNNNNNNNTGG 3 cut(s) 3383, 4034, 6175
XhoI CTCGAG 1 cut(s) 368
XmnI GAANNNNTTC 2 cut(s) 758, 3411
ZrmI AGTACT 1 cut(s) 4497
Zsp2I ATGCAT 4 cut(s) 3246, 4181, 5053, 6138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.