RLG00000002070

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000800
Physical Location & Seq
Reverse (-)
25001453 .. 25017414
15962 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002070

Sequence Viewer

Length: 6432 bp
ATGGCCTCCGCCACCGAAACCTCCGAAACTCTCCTCTCCAAGCTCGAATCAGCCGATTCCGCCGGGATTCATCTCCTCATCTCCGACTACCTAAGCCCATTCGCCGACCTTCACAAAAAGAAGACCTCAACCGACCACCAAACCCTACAACGCTCTCTCGCCAAAAAATTCACCCCCTTCCTCAACCGCGCCTTAGCCATCCTCCCCAAACGGCTCGCTGATCCTTCAAAGCTAGATGCCAAATTTGCCCCCGAATTGTTCGATATTTACCGCCTCTGCCTCGACTACATGGACGCCGTCTCCTCCCAATTTTCCGGCAAGCCCTACAAGTTTCACATCCAGAGGATCCGGTTGGTTTATGCATTCGAGGCCTGGGGCCGCCATAAGGACGCCGAGTCCGAGGCGCTTAGGGTTTTGAAGAGCTTCAAGGCGATTGAATTAGGAACGAAACCGGTGAATTTGGACCGTAGATTTGTGCCAGGTGTCGAGCAGGGAGGCTGCGATAAGGAGTTTGGGGCTTTGCTCGGAGAAGTAGTGTGGATGCTAGCCAAGTGTGCTTCGTTAATTCAAAGCAAGGAAGCCGAGGTGTATGAGCGGCTGCTTTGTATGGTTGAGGAGTTGGGACCTTGGTTTAGGGTGTTAGATGAAACTGTACATGACAAGGTGCACAGACAGCTTGCCATTTATCTCGAGAGGTGTACTCTTTTTTTAGTCCGGGAGCTATCATCCTTGGGAGCAAATCTCGTATGCAAGTTCTGCCGCATAACAGTGAGCGAGTATGCAAAGTCATCCATCAAAGATCATATGTTCAAGTGCGCCCGTCGAATATGTTCAGCCATATTTTCATTGCAAGAGACTAGTTCCTCACTGATCAGTAACACATTGTTATGCTTGTTTGACTCCCTTGTTGATGAATGCAAGGCTGAGGTCGAATATTCAGGAAAAGATTTTATTGAACTTATTGCTTATTGTGCCAATAAGTGTCGAACTGCAAGTACAAATCTTTCTGGTACCATGAGATCTCTTTCAAAAAAAATAATAACTCACTTTGATCAGGATCCACCTGTAAATTTGATTCTAAGACTTTATGCTACTGGTTTGCACTACGTTGATTGTAGTATAAAGTCAAAGGATGAGGATCTTACATCTTCTGGAGGTGCAATCAGAATGTTGCTTGATGATGAGGATACAATGACTGATTTGTCAGATTGTCTTGATTCATTAGAAAGTTATTTCCAAATTGGTACAAATGATATTTCCAAAAGTGGGTTTTCCACGAGTCACGTGCAGAAGGATTTAAAGGATTATCTTCTGCCTTATTTTAATGCATTGGAGTTCTTATGCCATCCACTTGCTGACTTAGTATATTCGGGAAAGAAACAGATTTTTGCTGATAAAGGAGCTGCCTCTGTTTCTAGTGAAATGTGTGTTATCCAGGGCGCATTTCATCAGTTATATGATTTGTTCCTTTCTTTACAAACGTACAGAATTACTTGTGAGATAGATATAGATGGACTTAAGCACAATAGCATCCTTCGTGCTTCTGTGGCTGCTTTTATACTTTCCACCAGAATGAAGCATAACATGCAGAAGAGTGCACAAATACTGAACCATGTCATTACCAGCGAATGGATTCAACTTGATGGTCTAAAGCATATATATGTCTCTCTCTACAATACTGGTACACGTTTGTACAAAAACAAGGAGCTGAATGAGGCTTCAGAGGCTTTGAAGCTTTCCTGCAAGGCATCTTGGACTCGTGTTATACGTCTCTGTGAACTGTTTTCAGGCAAATTAAAGGTCGCCAGTGCTGATCTTTCTGAAGATACTATTTTAGATGTCTTGAATGAGTCGTGTACAGGAAGTTCTTTTCTATTGGATATTCTTAACCAAGTTGATAATCATGAGGTGAAAAGAGCTATGGCAGAGTGCCTTGAAACTTGGTCTATTGCAGCAAATTTGTTTGGGAGGCTGCCAGGTCCAATGTCTGTGGTGAAGCAGTGGGTTAAGATGGAATGTAAATGCTGTAACAATATGCATATTGAGGACAGCGGTCCAACCTTATACAGTCTGCTGTCATCTTCTAAGAAAATATCAAAGAAGACAACTGAGATAATCTTGGAGCAGGGACTTCTTGCTTACGAGAAAATGTATGCTGTGAACCCCGAGTTTTGTCAGAAGATGCAAATGCAAATTATAAACATACTACTGCAAGATGTGTATGTGACACCAGACAGTTGGTTACAAAAAGCAAGAATTTTAGTGAGAAAGGGAAGAGCGTTAAAACTTTATGGGATCAAAGGTCTAAAAGAGTGTATTCATTGTTTATCAGAAGCAATATCTTCACTTAGTAAGATGTACGATAACACCAGTATCCATCAAACATCTCCTCGCCATGAATTAGCTGTGGCATATTGCTTACGCGCGCTTTGTACCCAGGAGGATGAACCACAATCAGAGAAAATGTTTCAAGATATCCGTGCTGCCCTTGATGCATGGTTGGGCATTTCTACTACAGACATGTGCTCTCCAGATGACAAATGCTCTCCAGATGACAAATGTTTAATGGTGTCTGAAAATACGATAATGCTCCTATACAATATTATTGATTTGTTGTCTATCAAGGGTCACATGGATTATTTCCATGATATATATAGGATTTTGATTAGATTATTTAACTGGAAAAATATCCCGTTAGAGAAGTGCTTGGTCAGATTGTGGGAGTGCAGGAGAGTTAGTCATGCCCTTTGTACTTCACCTGTTAATGATGCACTCATCATGAGCTTATCAGATCATTTTGGTGAACTTTCAAAGTCTATTGATTTTTGGATAGATTGTCTTAAAGGATCCCAACAATTGGTAATAGGCTTCCAACATAATTTCTCATTTCTATTTGCAAATTTACCTTGGGGCCCATGCAATCATGAAAGCTTGTTCCAATGTGAAATCACAGTTGATGAAGTTAAAGAAGCTGCTCTTGAACTTATTTCAAGTGTTCCTGTATCAAGTCAGTCGGCTTATGTAGCTGCATATCTCTACTATGATTTATGTGAAAGACTAGTTTCAAATGGACAGTTAGTTGAGGCTCTGTCATATGCAATGGAAGCTCACCAGTTACGCCTTGAACTATTTCAAAAGAAGTTTGCGTACTCTTTTGTACAAAAGGCTGAAAAGTACAATGATACTAGGGATACCTATCAGAACTTTACACTCGAAGATCATAATCTGGAAGTTAAAAGATCAGTAGCCCGTGAAGTTTGGTCCTTTGACACTAGTTCATGTAACTTGGAAAACTGTTATCTTAGTCCCTATAATGTACTTCAATGTTATCTTGAAAGTACTCTTCAGGTAGGGATTATCCATGAAATAGTTGGAAATGGAGCTGAGGCTGAGGTAGTTTTACAGTGGGGAAAGACTATTTCCTGCTCACAAAGCTTGCCACTATTTGCAGTTGCATTTTGTTCGGTCTTGGGAAAACTGTATCATAAGAAGCAGCTTTGGGATTTGGCAGAGAAGGAACTTCAAAGTGCCAACCAATATTTGAAGGCTAGTGCAAAAGATATTTCTTGCGTAAAGTGCAGATTGATGCAGAAAGCAACTGTTAAAGTGAATCTTGGTGATTTATACCAAAGCAAAGTTTATAGTTCAAGGGGTGCATCTTTAGAGAAATTGTCCTATGCTGAAAGTTTGTACAAATCAGCACTTACCAAGCTAAACCTTTCTGAGTGGCAAAATTCTGTTAGTTGCCCTGTACTTAAAGAAGTTGGATATTCTGCTGGCAATATTTCTAGTCATTTTGCGGAAACCAAGGTGGAACCAAAAAGCAAGATAGAAGCTAAAAAGTCTAGGACTAAGAATGCCCGAAAACCAGTGGGGAGGGATCAAGATTCAGTTCCTGAGTATAATTTGAGGTCAACTCAGTCTAGATGCCAATCTTCTCAGAACCAAAGTGTAAGAGGTACTGGTGTCCAAGTTGGTCATCCAAAACATTTGAAACGAAGCACTGAGTGTGATTCTTCTGACAATTTAAGTAACAAGGACTTTCTTTTGGATTTGAAAACTTGTGAGGTTTCATATGGGTCTGACCGAACATGCATTTGCAACAAAATGAGGTGTTGGCATTGTCTTCCCATGGAAGTTATGGAATCTGGGCTACTGAAAAACTTTGTAGATTTGAAATGGGAGTATGTCCGCAGGCGTCTTTCTCTCAGGCTTCTGACAGGACTAGGAAATTGTTTGGAGAATCAAGGTCAAATTCGTGAAACAAATGAATTTATCCTGCAAAGCGTATCAATTTTAGTCAGCAGGAACCCATTTTGTATAACTACCTCATTGATTCCGTCGACTTCCTTGCTTGATTTAATGGCAAAGGAAATCCCTGGGGATGTGCTTAGTGTTGAGCGTGCGGAAGTACTGTACAGCATATGTTGGCTATCTTTAAAGATTCGTTCCAAGAATACCAGGGTTATTTTCTCTGATCTGCCTCATATTCATCTACCAAAACTAGTGTCTTGGCTGATGTTAGCTTTTGTGCTTTGCCGTGAAGTTCCTGTACTTTTTCAGAAGGTTTCTAGATTGCTTGCTGCTATATTTTTACTTTCTGCCTCAAGTGAGCTTTTTTCTTTGTCATCTTCTTGCAAAAAGCTCCACGAAAATCATTGGGCTTCCTATTTTCATCAAGCTTCAATTGGCACCCATCTCAATTACCAATTTTTCATGAAAGTATCAGAGAGGTGTAAACTCCAACACCTTTTAAACAGGGAGGGGGCACATGTTGCTGGCTCATGCCTGGTATCAGAAACACAGAATTTACACAGGGTCGCTCCAGAGTCAATTCAAGACCTTGAAGAATTTGTGACAGAATTCTTTTCAGGCCTTCCATGTACCACAATCATCTGTATAAGTGTGCTTGGAGGCCCTTATGCTAGTTTGTTACAAGAATTGTTGTGTTTTCCTTCACGCATGCATGCATGGATTCTGGTTTCACGCTTGAACTCTAAGAATCAACCTATCAGTATGCTTCTGCCAGTGGATTCAGTATTGGAAGGAGATTCAGATGATGTTAGTTATACTGGAATAAAGGATTGGCACTGTCCATGGGGTTCCACTGTGGTTGATGATGTAGCTCCAGAGTTTCGATTGATATTGGAGGGGACTCATTCGTCGTCTGTGAAACATCCTGTACAAGATACAAATGAAAAAAAGCTTAACTGGTGGGTGCAGAGGAAGGATTTTGATCGCCGCCTTGGTAAATTTCTGAAGAACTTGGAAGATTCATGGTTTGGTACTTGGAAACTTGTCCTACTGGGTGAATGGTCAAACTGCAAGCAGTTGGACTTGGTACATAAGAAGCTGGTGCGCAATCTGAAATCCAAATGCAAAATGGAGATAGATGAGAGTCTTTTAAAAGTCATCCTTGGGGGTTCCAAATATGCCTTCGAAGGAGGTGCACATGTTACACAGCTTTGTCTTAAGAAAGGTTCCTACGTTAGTAAAGTTGGATATTTAGAGGAAGAGAAGTGTATGACATCATGTAATGATTCTAATGGTGGTGAGAACTTGTCTGAGTTGGCCTCAAAACTAGTTTTAGAAGCAGTGAATGAGCTTGAAGGTTTACATTCTTCTGTAAATATAGAGCCAACAATATTAGTTGTGGACTACGAGGTGCAGATGCTTCCTTGGGAGAATTTACCAATCCTAAGAAACCAGGAGGTTTATCGCATGCCCTCTGTTGGGAGCATTTTGGCAACAGCCAATAGAAACTACCACAATCAAGAAGTTCAGAGTATTGTGACTTCATTTCCTTTGATAGATCCCTTGGATGCCTTTTATCTTTTGAATCCCAGTGGTGATCTAAATTATACACAAAATGAATTTGAGACATGGTTCAGAGATCAAAACTTGGAGGGGAAGGCCGGGTGTGCACCTCCTGCTGAAGAATTGGCAGTAGCGTTGAAAAGCCATGATCTATTTATGTATTTTGGCCATGGATGTGGGCAACAATATATTCCAAGACGTGAGATTCAGAGACTGGAACACTGTGCCGCAACTCTCCTTATGGGATGCAGTAGTGGTTCTCTGAAACTGAATGGATGTTATGTTCCACAAGGTACTCCACTATCTTATCTGCTGGCCGGTTCTCCTGTCATTGTTGCCAATTTGTGGGATGTGACGGACAGGGATATTGACCGATTTGCAAAGTCCATGCTTGATTCTTGGTTGAAAGCAAGGTCGAGTCCCTGTGTGAGTTGTGCCCAATGCAACAAGAAAGTCACAAAAAAGAAATTGGCCACAAACCTGTCTTGTGAGCATAGGCCGAAGGTTGGATCATTCATGAGCGAAGCACGTAAAACCTGCCAGTTACCTTTCTTGATTGGAGCAGCACCAGTATGCTATGGTGTCCCAACTGGCATATGGAAAAAGGAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

2144

Amino Acids

241.87

Weight (kDa)

6.81

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 9 - 279 5.5e-104 Separase-like, TPR repeats
TPR_ESP1_2nd PF25113 289 - 462 6.2e-39 Separase-like, TPR repeats
Separin_C PF03568 1656 - 2062 1.3e-98 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2198
AasI GACNNNNNNGTC 2 cut(s) 1201, 5161
Acc16I TGCGCA 1 cut(s) 5360
Acc36I ACCTGC 1 cut(s) 6362
Acc65I GGTACC 1 cut(s) 1010
AccB1I GGYRCC 2 cut(s) 1010, 4691
AccB7I CCANNNNNTGG 3 cut(s) 1629, 2857, 6162
AccBSI CCGCTC 1 cut(s) 595
AccI GTMKAC 1 cut(s) 4343
AccII CGCG 3 cut(s) 189, 2424, 2426
AcoI YGGCCR 3 cut(s) 5983, 6132, 6288
AcuI CTGAAG 5 cut(s) 1704, 1842, 3329, 5279, 5955
AcvI CACGTG 1 cut(s) 1285
AcyI GRCGYC 3 cut(s) 294, 390, 4198
AdeI CACNNNGTG 1 cut(s) 4008
AfiI CCNNNNNNNGG 8 cut(s) 385, 1266, 1629, 2857, 5732, 5733, 6162, 6323
AflII CTTAAG 2 cut(s) 1517, 5471
AflIII ACRYGT 4 cut(s) 1685, 2520, 4771, 5452
AgeI ACCGGT 1 cut(s) 451
AhdI GACNNNNNGTC 1 cut(s) 394
AhlI ACTAGT 5 cut(s) 857, 3058, 3272, 4504, 5581
AjiI CACGTC 1 cut(s) 6017
AjnI CCWGG 9 cut(s) 371, 478, 1434, 1975, 2436, 4378, 4460, 4788, 5706
AjuI GAANNNNNNNTTGG 4 cut(s) 5068, 5100, 5420, 5452
AloI GAACNNNNNNTCC 2 cut(s) 6084, 6116
Alw21I GWGCWC 5 cut(s) 669, 1600, 2528, 5452, 5926
Alw26I GTCTC 6 cut(s) 304, 848, 1669, 1775, 5873, 6022
Alw44I GTGCAC 4 cut(s) 665, 1596, 5448, 5922
AlwNI CAGNNNCTG 2 cut(s) 3896, 6031
Ama87I CYCGRG 2 cut(s) 689, 2165
ApaI GGGCCC 1 cut(s) 2915
ApaLI GTGCAC 4 cut(s) 665, 1596, 5448, 5922
ArsI GACNNNNNNTTYG 6 cut(s) 455, 487, 1785, 1817, 4493, 4525
AsiGI ACCGGT 1 cut(s) 451
Asp700I GAANNNNTTC 4 cut(s) 422, 829, 1632, 3129
Asp718I GGTACC 1 cut(s) 1010
AspLEI GCGC 7 cut(s) 191, 406, 818, 1442, 2426, 2428, 5361
AspS9I GGNCC 9 cut(s) 376, 463, 623, 1979, 2054, 2911, 2912, 3261, 4916
AsuC2I CCSGG 3 cut(s) 64, 716, 5917
AsuII TTCGAA 1 cut(s) 5439
AsuNHI GCTAGC 1 cut(s) 544
AvaI CYCGRG 2 cut(s) 689, 2165
AvaII GGWCC 5 cut(s) 463, 623, 1979, 2054, 3261
BaeGI GKGCMC 7 cut(s) 669, 1600, 2915, 4771, 5452, 5926, 6256
BaeI ACNNNNGTAYC 4 cut(s) 2356, 2389, 3951, 3984
BalI TGGCCA 2 cut(s) 5985, 6290
BamHI GGATCC 3 cut(s) 345, 1057, 2846
BanI GGYRCC 2 cut(s) 1010, 4691
BanII GRGCYC 1 cut(s) 2915
BarI GAAGNNNNNNTAC 6 cut(s) 3131, 3163, 4536, 4568, 5779, 5811
BauI CACGAG 2 cut(s) 1276, 1758
BbrPI CACGTG 1 cut(s) 1285
BbsI GAAGAC 3 cut(s) 128, 2108, 4118
Bbv12I GWGCWC 5 cut(s) 669, 1600, 2528, 5452, 5926
BbvCI CCTCAGC 3 cut(s) 924, 3384, 3390
BccI CCATC 8 cut(s) 206, 800, 1353, 1505, 1637, 2005, 2385, 4704
BceAI ACGGC 3 cut(s) 227, 281, 4524
BcgI CGANNNNNNTGC 8 cut(s) 1267, 1301, 3444, 3478, 4333, 4367, 5429, 5463
BciT130I CCWGG 9 cut(s) 373, 480, 1436, 1977, 2438, 4380, 4462, 4790, 5708
BciVI GTATCC 3 cut(s) 1180, 2384, 3184
BclI TGATCA 2 cut(s) 870, 1051
BcnI CCSGG 3 cut(s) 64, 716, 5917
BcoDI GTCTC 6 cut(s) 304, 848, 1669, 1775, 5873, 6022
BcuI ACTAGT 5 cut(s) 857, 3058, 3272, 4504, 5581
BfmI CTRYAG 1 cut(s) 2514
BfoI RGCGCY 1 cut(s) 407
BfrI CTTAAG 2 cut(s) 1517, 5471
BfuAI ACCTGC 1 cut(s) 6362
BfuI GTATCC 3 cut(s) 1180, 2384, 3184
BglII AGATCT 1 cut(s) 1019
BmcAI AGTACT 2 cut(s) 3340, 4413
Bme18I GGWCC 5 cut(s) 463, 623, 1979, 2054, 3261
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 2 cut(s) 689, 2165
BmgBI CACGTC 1 cut(s) 6017
BmgT120I GGNCC 9 cut(s) 376, 463, 623, 1979, 2054, 2911, 2912, 3261, 4916
BmrI ACTGGG 2 cut(s) 5315, 5838
BmtI GCTAGC 1 cut(s) 548
BmuI ACTGGG 2 cut(s) 5315, 5838
BoxI GACNNNNGTC 1 cut(s) 2052
BpiI GAAGAC 3 cut(s) 128, 2108, 4118
BplI GAGNNNNNCTC 8 cut(s) 685, 717, 726, 758, 3901, 3933, 5558, 5590
BpmI CTGGAG 5 cut(s) 1173, 2514, 2532, 4809, 5112
Bpu10I CCTNAGC 6 cut(s) 92, 193, 407, 924, 3384, 3390
Bpu14I TTCGAA 1 cut(s) 5439
BpuEI CTTGAG 1 cut(s) 4591
BpuMI CCSGG 3 cut(s) 64, 716, 5917
BsaAI YACGTR 2 cut(s) 1285, 6347
BsaBI GATNNNNATC 6 cut(s) 1056, 1137, 3195, 3222, 3931, 5235
BsaHI GRCGYC 3 cut(s) 294, 390, 4198
BsaWI WCCGGW 2 cut(s) 348, 451
BsaXI ACNNNNNCTCC 6 cut(s) 284, 314, 519, 549, 2722, 2752
Bsc4I CCNNNNNNNGG 8 cut(s) 385, 1266, 1629, 2857, 5732, 5733, 6162, 6323
Bse118I RCCGGY 2 cut(s) 451, 6134
Bse3DI GCAATG 2 cut(s) 845, 3105
Bse8I GATNNNNATC 6 cut(s) 1056, 1137, 3195, 3222, 3931, 5235
BseBI CCWGG 9 cut(s) 373, 480, 1436, 1977, 2438, 4380, 4462, 4790, 5708
BseJI GATNNNNATC 6 cut(s) 1056, 1137, 3195, 3222, 3931, 5235
BseLI CCNNNNNNNGG 8 cut(s) 385, 1266, 1629, 2857, 5732, 5733, 6162, 6323
BseMI GCAATG 2 cut(s) 845, 3105
BsePI GCGCGC 1 cut(s) 2424
BseRI GAGGAG 5 cut(s) 23, 65, 292, 629, 2379
BseSI GKGCMC 7 cut(s) 669, 1600, 2915, 4771, 5452, 5926, 6256
BsgI GTGCAG 5 cut(s) 1307, 2746, 3598, 5240, 5687
Bsh1236I CGCG 3 cut(s) 189, 2424, 2426
BshNI GGYRCC 2 cut(s) 1010, 4691
BshTI ACCGGT 1 cut(s) 451
BsiHKAI GWGCWC 5 cut(s) 669, 1600, 2528, 5452, 5926
BsiHKCI CYCGRG 2 cut(s) 689, 2165
BsiSI CCGG 7 cut(s) 63, 315, 349, 452, 715, 5916, 6135
BslFI GGGAC 6 cut(s) 636, 2142, 3291, 5167, 6222, 6386
BslI CCNNNNNNNGG 8 cut(s) 385, 1266, 1629, 2857, 5732, 5733, 6162, 6323
BsmAI GTCTC 6 cut(s) 304, 848, 1669, 1775, 5873, 6022
BsmBI CGTCTC 2 cut(s) 304, 1775
BsmFI GGGAC 6 cut(s) 636, 2142, 3291, 5167, 6222, 6386
BsmI GAATGC 3 cut(s) 362, 920, 3862
BsoBI CYCGRG 2 cut(s) 689, 2165
Bsp119I TTCGAA 1 cut(s) 5439
Bsp120I GGGCCC 1 cut(s) 2911
Bsp1286I GDGCHC 8 cut(s) 669, 1600, 2528, 2915, 4771, 5452, 5926, 6256
Bsp1407I TGTACA 7 cut(s) 652, 1692, 1856, 3157, 3690, 4416, 5182
Bsp19I CCATGG 3 cut(s) 4131, 5096, 5986
BspFNI CGCG 3 cut(s) 189, 2424, 2426
BspHI TCATGA 5 cut(s) 1903, 2778, 2923, 4716, 6333
BspMI ACCTGC 1 cut(s) 6362
BspOI GCTAGC 1 cut(s) 548
BspQI GCTCTTC 2 cut(s) 413, 2269
BspT104I TTCGAA 1 cut(s) 5439
BspT107I GGYRCC 2 cut(s) 1010, 4691
BspTI CTTAAG 2 cut(s) 1517, 5471
BsrBI CCGCTC 1 cut(s) 595
BsrDI GCAATG 2 cut(s) 845, 3105
BsrFI RCCGGY 2 cut(s) 451, 6134
BsrGI TGTACA 7 cut(s) 652, 1692, 1856, 3157, 3690, 4416, 5182
BssAI RCCGGY 2 cut(s) 451, 6134
BssHII GCGCGC 1 cut(s) 2424
BssNI GRCGYC 3 cut(s) 294, 390, 4198
BssSI CACGAG 2 cut(s) 1276, 1758
Bst2BI CACGAG 2 cut(s) 1276, 1758
Bst2UI CCWGG 9 cut(s) 373, 480, 1436, 1977, 2438, 4380, 4462, 4790, 5708
Bst6I CTCTTC 5 cut(s) 413, 1586, 2269, 3348, 5508
BstACI GRCGYC 3 cut(s) 294, 390, 4198
BstAFI CTTAAG 2 cut(s) 1517, 5471
BstAPI GCANNNNNTGC 4 cut(s) 756, 1585, 4775, 5930
BstAUI TGTACA 7 cut(s) 652, 1692, 1856, 3157, 3690, 4416, 5182
BstBAI YACGTR 2 cut(s) 1285, 6347
BstBI TTCGAA 1 cut(s) 5439
BstDSI CCRYGG 3 cut(s) 4131, 5096, 5986
BstFNI CGCG 3 cut(s) 189, 2424, 2426
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 7 cut(s) 191, 406, 818, 1442, 2426, 2428, 5361
BstMAI GTCTC 6 cut(s) 304, 848, 1669, 1775, 5873, 6022
BstNI CCWGG 9 cut(s) 373, 480, 1436, 1977, 2438, 4380, 4462, 4790, 5708
BstNSI RCATGY 8 cut(s) 1588, 2524, 4095, 4775, 4966, 4970, 5456, 5725
BstPAI GACNNNNGTC 1 cut(s) 2052
BstSFI CTRYAG 1 cut(s) 2514
BstSLI GKGCMC 7 cut(s) 669, 1600, 2915, 4771, 5452, 5926, 6256
BstUI CGCG 3 cut(s) 189, 2424, 2426
BstV2I GAAGAC 3 cut(s) 128, 2108, 4118
BstX2I RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2846, 5812
BstXI CCANNNNNNTGG 2 cut(s) 2238, 5993
BstYI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2846, 5812
BsuI GTATCC 3 cut(s) 1180, 2384, 3184
BtgI CCRYGG 3 cut(s) 4131, 5096, 5986
BtrI CACGTC 1 cut(s) 6017
BtsI GCAGTG 2 cut(s) 2006, 5601
BveI ACCTGC 1 cut(s) 6362
CaiI CAGNNNCTG 2 cut(s) 3896, 6031
CciI TCATGA 5 cut(s) 1903, 2778, 2923, 4716, 6333
CfoI GCGC 7 cut(s) 191, 406, 818, 1442, 2426, 2428, 5361
Cfr10I RCCGGY 2 cut(s) 451, 6134
Cfr13I GGNCC 9 cut(s) 376, 463, 623, 1979, 2054, 2911, 2912, 3261, 4916
CseI GACGC 3 cut(s) 302, 398, 4187
CspAI ACCGGT 1 cut(s) 451
CspCI CAANNNNNGTGG 4 cut(s) 1971, 2006, 5095, 5130
DraI TTTAAA 4 cut(s) 1299, 4440, 4755, 5406
DraIII CACNNNGTG 1 cut(s) 4008
DrdI GACNNNNNNGTC 2 cut(s) 1201, 5161
DriI GACNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 2 cut(s) 1201, 5161
EaeI YGGCCR 3 cut(s) 5983, 6132, 6288
Eam1104I CTCTTC 5 cut(s) 413, 1586, 2269, 3348, 5508
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 5 cut(s) 413, 1586, 2269, 3348, 5508
EciI GGCGGA 1 cut(s) 49
Eco147I AGGCCT 2 cut(s) 371, 4875
Eco24I GRGCYC 1 cut(s) 2915
Eco32I GATATC 1 cut(s) 2476
Eco47I GGWCC 5 cut(s) 463, 623, 1979, 2054, 3261
Eco57I CTGAAG 5 cut(s) 1704, 1842, 3329, 5279, 5955
Eco72I CACGTG 1 cut(s) 1285
Eco88I CYCGRG 2 cut(s) 689, 2165
EcoO109I RGGNCCY 3 cut(s) 623, 2911, 4916
EcoRI GAATTC 1 cut(s) 4862
EcoRII CCWGG 9 cut(s) 371, 478, 1434, 1975, 2436, 4378, 4460, 4788, 5706
EcoRV GATATC 1 cut(s) 2476
EcoT22I ATGCAT 7 cut(s) 364, 1330, 2040, 2497, 4097, 4968, 4972
EcoT38I GRGCYC 1 cut(s) 2915
Esp3I CGTCTC 2 cut(s) 304, 1775
FalI AAGNNNNNCTT 6 cut(s) 2961, 2993, 3597, 3629, 5400, 5432
FaqI GGGAC 6 cut(s) 636, 2142, 3291, 5167, 6222, 6386
FauNDI CATATG 5 cut(s) 804, 3094, 4075, 4424, 6413
FbaI TGATCA 2 cut(s) 870, 1051
FblI GTMKAC 1 cut(s) 4343
FriOI GRGCYC 1 cut(s) 2915
FspI TGCGCA 1 cut(s) 5360
GlaI GCGC 7 cut(s) 190, 405, 817, 1441, 2425, 2427, 5360
GsuI CTGGAG 5 cut(s) 1173, 2514, 2532, 4809, 5112
HaeII RGCGCY 1 cut(s) 407
HapII CCGG 7 cut(s) 63, 315, 349, 452, 715, 5916, 6135
HgaI GACGC 3 cut(s) 302, 398, 4187
HhaI GCGC 7 cut(s) 191, 406, 818, 1442, 2426, 2428, 5361
Hin1I GRCGYC 3 cut(s) 294, 390, 4198
Hin6I GCGC 7 cut(s) 189, 404, 816, 1440, 2424, 2426, 5359
HinP1I GCGC 7 cut(s) 189, 404, 816, 1440, 2424, 2426, 5359
HincII GTYRAC 2 cut(s) 3915, 4344
HindII GTYRAC 2 cut(s) 3915, 4344
HindIII AAGCTT 5 cut(s) 1733, 2929, 3433, 4680, 5204
HpaII CCGG 7 cut(s) 63, 315, 349, 452, 715, 5916, 6135
Hpy99I CGWCG 3 cut(s) 825, 4345, 5167
HpyCH4IV ACGT 8 cut(s) 1107, 1284, 1481, 1687, 1768, 5487, 6016, 6346
HpySE526I ACGT 8 cut(s) 1107, 1284, 1481, 1687, 1768, 5487, 6016, 6346
Hsp92I GRCGYC 3 cut(s) 294, 390, 4198
HspAI GCGC 7 cut(s) 189, 404, 816, 1440, 2424, 2426, 5359
KpnI GGTACC 1 cut(s) 1014
Ksp22I TGATCA 2 cut(s) 870, 1051
LguI GCTCTTC 2 cut(s) 413, 2269
MaeII ACGT 8 cut(s) 1107, 1284, 1481, 1687, 1768, 5487, 6016, 6346
MbiI CCGCTC 1 cut(s) 595
MfeI CAATTG 2 cut(s) 2855, 4686
MflI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2846, 5812
MhlI GDGCHC 8 cut(s) 669, 1600, 2528, 2915, 4771, 5452, 5926, 6256
MlsI TGGCCA 2 cut(s) 5985, 6290
MluNI TGGCCA 2 cut(s) 5985, 6290
MlyI GAGTC 9 cut(s) 404, 893, 1288, 1750, 1859, 4838, 5149, 5407, 6244
MmeI TCCRAC 9 cut(s) 108, 2081, 2896, 3352, 3745, 4768, 5313, 5479, 6304
Mox20I TGGCCA 2 cut(s) 5985, 6290
Mph1103I ATGCAT 7 cut(s) 364, 1330, 2040, 2497, 4097, 4968, 4972
MroXI GAANNNNTTC 4 cut(s) 422, 829, 1632, 3129
MscI TGGCCA 2 cut(s) 5985, 6290
MslI CAYNNNNRTG 7 cut(s) 767, 886, 1571, 1659, 2799, 5991, 6388
Msp20I TGGCCA 2 cut(s) 5985, 6290
MspA1I CMGCKG 1 cut(s) 2052
MspCI CTTAAG 2 cut(s) 1517, 5471
MspI CCGG 7 cut(s) 63, 315, 349, 452, 715, 5916, 6135
MunI CAATTG 2 cut(s) 2855, 4686
Mva1269I GAATGC 3 cut(s) 362, 920, 3862
MvaI CCWGG 9 cut(s) 373, 480, 1436, 1977, 2438, 4380, 4462, 4790, 5708
MvnI CGCG 3 cut(s) 189, 2424, 2426
NciI CCSGG 3 cut(s) 64, 716, 5917
NcoI CCATGG 3 cut(s) 4131, 5096, 5986
NdeI CATATG 5 cut(s) 804, 3094, 4075, 4424, 6413
NheI GCTAGC 1 cut(s) 544
NmeAIII GCCGAG 2 cut(s) 418, 607
NmuCI GTSAC 7 cut(s) 1280, 2224, 2627, 4855, 5791, 6169, 6271
NsbI TGCGCA 1 cut(s) 5360
NsiI ATGCAT 7 cut(s) 364, 1330, 2040, 2497, 4097, 4968, 4972
NspI RCATGY 8 cut(s) 1588, 2524, 4095, 4775, 4966, 4970, 5456, 5725
NspV TTCGAA 1 cut(s) 5439
PaeI GCATGC 3 cut(s) 4966, 4970, 5725
PaeR7I CTCGAG 1 cut(s) 689
PagI TCATGA 5 cut(s) 1903, 2778, 2923, 4716, 6333
PasI CCCWGGG 1 cut(s) 4379
PauI GCGCGC 1 cut(s) 2424
PceI AGGCCT 2 cut(s) 371, 4875
PciI ACATGT 3 cut(s) 2520, 4771, 5452
PciSI GCTCTTC 2 cut(s) 413, 2269
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 396, 1765
PctI GAATGC 3 cut(s) 362, 920, 3862
PdmI GAANNNNTTC 4 cut(s) 422, 829, 1632, 3129
PflFI GACNNNGTC 1 cut(s) 296
PflMI CCANNNNNTGG 3 cut(s) 1629, 2857, 6162
PfoI TCCNGGA 1 cut(s) 714
PinAI ACCGGT 1 cut(s) 451
PleI GAGTC 9 cut(s) 403, 893, 1287, 1750, 1858, 4837, 5149, 5406, 6243
PmaCI CACGTG 1 cut(s) 1285
PmlI CACGTG 1 cut(s) 1285
PpsI GAGTC 9 cut(s) 403, 893, 1287, 1750, 1858, 4837, 5149, 5406, 6243
Ppu21I YACGTR 2 cut(s) 1285, 6347
PpuMI RGGWCCY 1 cut(s) 623
PscI ACATGT 3 cut(s) 2520, 4771, 5452
PshAI GACNNNNGTC 1 cut(s) 2052
PsiI TTATAA 1 cut(s) 2198
Psp5II RGGWCCY 1 cut(s) 623
Psp6I CCWGG 9 cut(s) 371, 478, 1434, 1975, 2436, 4378, 4460, 4788, 5706
PspCI CACGTG 1 cut(s) 1285
PspGI CCWGG 9 cut(s) 371, 478, 1434, 1975, 2436, 4378, 4460, 4788, 5706
PspOMI GGGCCC 1 cut(s) 2911
PspPI GGNCC 9 cut(s) 376, 463, 623, 1979, 2054, 2911, 2912, 3261, 4916
PspPPI RGGWCCY 1 cut(s) 623
PsrI GAACNNNNNNTAC 4 cut(s) 979, 1011, 1849, 1881
PstNI CAGNNNCTG 2 cut(s) 3896, 6031
PsuI RGATCY 6 cut(s) 345, 1019, 1057, 1138, 2846, 5812
PsyI GACNNNGTC 1 cut(s) 296
PteI GCGCGC 1 cut(s) 2424
RseI CAYNNNNRTG 7 cut(s) 767, 886, 1571, 1659, 2799, 5991, 6388
SalI GTCGAC 1 cut(s) 4342
SapI GCTCTTC 2 cut(s) 413, 2269
Sau96I GGNCC 9 cut(s) 376, 463, 623, 1979, 2054, 2911, 2912, 3261, 4916
ScaI AGTACT 2 cut(s) 3340, 4413
SchI GAGTC 9 cut(s) 404, 893, 1288, 1750, 1859, 4838, 5149, 5407, 6244
SduI GDGCHC 8 cut(s) 669, 1600, 2528, 2915, 4771, 5452, 5926, 6256
SfcI CTRYAG 1 cut(s) 2514
Sfr274I CTCGAG 1 cut(s) 689
SfuI TTCGAA 1 cut(s) 5439
SinI GGWCC 5 cut(s) 463, 623, 1979, 2054, 3261
SlaI CTCGAG 1 cut(s) 689
SmiMI CAYNNNNRTG 7 cut(s) 767, 886, 1571, 1659, 2799, 5991, 6388
SmlI CTYRAG 4 cut(s) 689, 1517, 4606, 5471
SmoI CTYRAG 4 cut(s) 689, 1517, 4606, 5471
SpeI ACTAGT 5 cut(s) 857, 3058, 3272, 4504, 5581
SphI GCATGC 3 cut(s) 4966, 4970, 5725
SseBI AGGCCT 2 cut(s) 371, 4875
SspI AATATT 5 cut(s) 935, 2602, 3539, 3784, 5646
StuI AGGCCT 2 cut(s) 371, 4875
TaiI ACGT 8 cut(s) 1110, 1287, 1484, 1690, 1771, 5490, 6019, 6349
TaqII GACCGA 3 cut(s) 3454, 4101, 6204
TauI GCSGC 5 cut(s) 381, 598, 762, 5244, 6047
TseFI GTSAC 7 cut(s) 1280, 2224, 2627, 4855, 5791, 6169, 6271
Tsp45I GTSAC 7 cut(s) 1280, 2224, 2627, 4855, 5791, 6169, 6271
TspGWI ACGGA 3 cut(s) 2468, 4329, 6188
Tth111I GACNNNGTC 1 cut(s) 296
Van91I CCANNNNNTGG 3 cut(s) 1629, 2857, 6162
Vha464I CTTAAG 2 cut(s) 1517, 5471
VneI GTGCAC 4 cut(s) 665, 1596, 5448, 5922
VpaK11BI GGWCC 5 cut(s) 463, 623, 1979, 2054, 3261
XbaI TCTAGA 2 cut(s) 3923, 4571
XceI RCATGY 8 cut(s) 1588, 2524, 4095, 4775, 4966, 4970, 5456, 5725
XcmI CCANNNNNNNNNTGG 4 cut(s) 3368, 4138, 5380, 6411
XhoI CTCGAG 1 cut(s) 689
XmiI GTMKAC 1 cut(s) 4343
XmnI GAANNNNTTC 4 cut(s) 422, 829, 1632, 3129
ZrmI AGTACT 2 cut(s) 3340, 4413
Zsp2I ATGCAT 7 cut(s) 364, 1330, 2040, 2497, 4097, 4968, 4972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.