FvH4_3g35420

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000800
Physical Location & Seq
Reverse (-)
30477435 .. 30491557
14123 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g35420.t1

Sequence Viewer

Length: 6420 bp
ATGGCCTCCGCCGCGGAGACCTCTGAATCTCTCCTCTCCAAGCTCGAATCCGCCGATTCTGCCGGGATTCACCGCCTCATCTCCGACTACCTAAACCCATTCGCCGACCTTCACAACAAAAAGAAGAAGAAGACCTCCGCCGACCTCCAAACCCTACAACGCTCTCTCGCCAAAAAATTCACCCCCTTCCTCAACCGCGCCCTATCCATCCTCCCCAAACGCCTCGCCGATCCTTCAAACCTCGATGCCGCTTTTGCCCCCGAATTGTTCGATATTTACCGCCTCTGCCTCGACTCCATGGCCGCCGTGTCGTCGCAATTCTCCGGCAAGCCGTACAAGTTTCACATCCACAGGATCCGGTTCATTTGTGCCCTCGAGGCCTGGGCCCGCCACCGGGATGCGGAGTCCGAGGCGCTTAGGGTTTTGAAGAGCTTCAAGGCAATTCAATTAGGAACGGAGCCGGTGGATTTGGACAGTCGGTTTGTGCCAGGTGTCAAGCAGGGCTGCGCCGATAACGAGTTCGGGGCTCTGATCGGAGAAGTGGTGTATATTCTAGCGAGGTGTGCTTCTGCAATTCAAAGCAAGGAGGAGGAGGTATATGAGCGGCTGCTTTGTATGGTTGAGGAGTTGGCTCCTTGGTTTAGGGTGCTAGATGAAACTGCAAGTGACAAAGTACACAGACAGCTTGCCATTTATCTCGGGAGGTGTACTCTTTTTTTAGTCGAGACACTTTCATCCTTTGGAGAAAATCTCATATGCAAGTTCTGCTGCATAACAGTGAGCGAGTATGCAAAGTCGCCCATCAAAGATCATATGTTCAAGTGGGGACGTCGAATATGTTCAGCTTTATTTTCATTACAAGAGCCTGGTTCGTCACGGATCAGTAACACATTGTTATGCTTGTTGGACTCCCTTGTTGATGAATGCAAGGCTGAGATCGAATTTCCAGGAAAAGATTTTGCTGAACTTACTGCTTATTGTGCCAATAAGTGTTGTACTACAAGTACAAATCTTTCTGGTAGTTTGAGATCTCATTTAGAAAAGTTAATAACTCACTTTGATCAGGTTCCACCTTTAAATTTGATTCTAAGGCTTTATGCTACTGGTTTGCACTACGTTGATTGTAGTTTAAAGTCCAAGGATGATGATGTTACATCTTCTGGGGGTGCAATTAGAATGTTGCTTGATGATGGCGGTATAACGACTGATTTGTCAGATTGTCTTGATTCTTTAGAAAGTTATTTCCAAATTGGTACAAATGATATTTCCAAAATGGGGTTTTCCACGAGGCACGTGCAGAAGAATTTAAAGGATTATCTTCTGCCTTATTATAATGCATTGGAGTTCTTATGCCACCCACTTGTTGACATAGTTTATTCGGGAAAGAAGCAGATTTTTGCTGATAATGGAGCTGCCTTTGTTTCTAATGGACTGTGGGTTATCCAGGGTGCCTTTCATCAGTTATACGATGTGTTCCTTTCTTTACAAACGTACAGAATTACTTGTGATATAGATATAGATGGATTTAAGCAGAACAGCATCCTTCGTGCTTCTGTGGCTGCTTGTATACTTTCCACCAGAACGAAGCTTAACACACAGAGGAGTGCACAAATACTAAACCATGTCATTACCAGCGAATGGATTCAACTTGATGGTCTAAAGCATATATATGCCTCTCTCTACAGTACTGGGACACGTTTGTACAAAAACAAGGAGCTGACTGAGGCTTCAGAGGCTTTGAAGCTATCCTGCAAGGCATCTTGGGCTCGTGTTATACGTCTTTGTGAACTATTTGCAGGCAAATTAAAGGTCTCCATTGCTGATCTTTCCAAAGATACCATTTTAGATGTCTTGAATGAGTCATGTACAGTAAGCGCTTTTTTATTGGATATTCTTAACCAAGTTGATAATCACGAGGTGAAAAGAGCTATGGTAGAGTGCCTTGAAACTTGGTCTACTGCAGTAAATTTGTTTGGGAAGCTGCCAGGTCCAATGTCTGTGGTGAAGCAGTGGGTTAAGATGGAATGTAAGTGCTGTAACAATGTGCACATTGAGGATAGCTGTCCAACCTTATACAGTCTGCTGTCATCTTCTAAGAATTTATCAAAGAAGACGACTGAGATAATCTTGGAGCAGGGACTTCTTGCTTATGAGCAAATGTATGCTGTGAACCCTGAGTTTTGTCAGAAGATGCAAATGCAAATTATAAACATACTACTGCAAGATGTGTATGTCACTCCAGATAGTTGGTTGCAAAAAGCAAAAATATTAGTGAGAAAGGGAAGATGGTTAAAACTCTATGGAATCAATGGTCTAAAGGACTGTATCTGTTGTTTAACAGAAGCAATAACTTTACTTGTAAGCAATCAGAGTAAGACGTATGGTAACACCAGTATCCATGAAACATCTCCTCGACATGAATTAGCTGTGGCATATTGCTTACGAGCGCTTTGTACCCAGGAGGATGAACCGCAATCAGAGAAAATGTTTCAAGATATCCGTGCTGCCCTTGATGCATGGTTGGGAATTTCTACTATAGACATGTGCTCTCCAGACGACAAATGCTTCATGGTGTCTGAAAGTACGATGACACTCCTATATAATATTTTTGATTTGTTATCTATCAAGGGTCACATGGATTATTATCATGATATATATAGGATTTTGATTAGATTATATAACTGGAAAAATATCCCGTTAGAGAAGTGCTTGGTCAGATTGTGGGAGTGCAGGAGAGTTAGTCATGCCCTTTGTAGTTCGCCTGTTAATGATACACTCGTCATGAACTTATCAGATCATTTTGGTGAACTTCCAAAATCTATTGAGTTTTGGGTAGATTCTCTTAAAGGATCCCGACAATTGGTAATAGGGTTCCAACATAATTTCTCATTTCTATTTGCAAATGTACCTGGGAGACCATGCATTCCTAAAAACTTGTTCCGATGTGAAATCACAGTTGATGAAGTAAAAGAAGCTGCTCTTGAACTTATTTCAAGTGTTCCTGTTTCGAGTCAGTCGTCTTATGTAACTGCATATCTCTACTATGATTTATGTGAAAGACTAGTTTCAAACGGCCAGTTAGTTGAGGCTCTGTCATATGCAATGGAAGCTCACCAGTTGCGCCGTGAACTGTTCCAAAAGAAGTTTGCATACTGTTTTGTACAAAAGACTGAAAAGTACAATGAAACTGGGGATACCTATCAGAAGTTTACACTGAATGATCATAATCTGGTATTGCAAAGATCAGTTTCCCGTGAAGTTTGGTCCTTTGACACTAGTTCATGTAACTTGGAAAAGTGTTATCTTAGTCCTTATAATGTACTTCAATGTTATCTTGAAAGCACTCTTCAGGTAGGGGTTATCCATGAAATAATTGGTAATGGAGCTGAGGCTGAGGTACATTTACAGTGGGGAAAGACTATTTCCTGCTCACAAAGCTTGCCACTATTTACAGTTGCATTTTGTTCGGTCTTGGGAAAACTGTATCTTAAGAAGCAGCTTTGGGATTTGGCAGAAAAGGAACTGCAAAGTGCCAAGCAATATTTGGATGCTAGTGAAAAAGATATTTCTTGCTTAAAGTGCAGATTGATGCAAGAAGCAACTGTTGAGCTGAATCTTGCTGATTTATACCAAAGGAAATTGTATAGTTCAAGGAGTGCATCTTTAGAGAAACCATCTTGTGCTGAAAGTTTGTACAATTCAGCACTTACCAAGCTAAACCTTCCTGAGTGGAAAAACTCTGTTAGTTGCCCCGGACTAACTGTACTTAAAGAAGTTGGAAATTCTGGTGGCAGTACTTCTAGTCATTTTGCAGAAACCAAGGTGGAACCAAAAGGCAAGATAGAAGCTAAAAAGTCTAGGACTAAGAATGCCCGAAAACCAGTGGGGAGGGATCAAGTTTCAGTTCCTTACAATTTGAGGTCCACTCAGTCTAGATCCCAATCTTGTCATAACCAAAGTGTAAGAGGTACTGGTGTCCAAGTTGGTGATTCAAAAAATTCGAAACGAAGCACTGAGTGTGATTCTTCTGATAATTTAAGTAACAGAGACTTTCTTTTGGATTTGGAATGTTGTGAGGTTTCATATGGGTGTGACCAAACATGCATTTGCCACAAAACGAGGTGTTGGCAATGTCTTCCCATGGAAGTTATGGAATCTGGGCTACTGAAAAACTTTGTAGATTTGAAATGGGAGTATGTCCGCAGGCGTCTCTCTCTCAGGCTTCTGACAGGATTAGGAAATTGTTTGGAGCATCAAGGTCAAATTCATGAAGCAAATGAATTTATCCTGCAAAGCGTATCAGTTTTAGTCAGCAGGAACCCATTTTCTATGACTACCTCATCTGTTCCGCCAACTTGCTTGCTTGATCTAATGGCAAAGGAAATCCCTGGGGATGTGCTTAGTGTTGAGCGTGCGGAGATACTGTACAGCATATGTTGGCTGTCTTTAAAGATTCGTTCCAAGAATAAGAGGGTTCTTTTTTCTGATCTGCCTCATATCCATCTACCAAAACTAGTGTCATGGTTGATGTTAGCTTTTGTGCTCTGCCGTGAGGTTCCTGTACTTTTTCAGAAGGTTTCTAGATTGCTTGCGGCTATATTTTTACTTTCTGCCTCGAGTGAAACTTTTTCTCTGTCATCTTCTTGCAAAAATCTCCACGAAAATCATTGGGCTTCTTATTTCCATCAAGCTTCAATTGGCGCGCATCTCAATTACCATTTTTTTACGAAAATATCTGAGAGGTGTAAACTCCAACACCCTGTAAACAGTGAGGGGGCACATGTTGCTGGCTCATGCTTGGTACCAGAAAAACAGAATTTACATAGGGTCGCTCCTGAGTCAACTCAATACCTTGAAGAATTTGTGACAGAATTCTTTTCAGGCCTTCCATGCACCACAATCATCTGTATAAGTGTGCTTGGAGGCCCTTATGCTAGTTTGTTACAAGAATTGTTGTGTTTTCCATCACTTGTGCATGCATGGATTGTGGTTTCACGCTTGAACTCTAAGAATCAACCTATCAGTGTGCTTCTGCCAGTGGATTCAGTATTAGAAGGAGATTCAGATGATGATAGTTTTTCTGGAATAAAGAATTGGCACTGTCCATGGGGTTCCACTGTGGTTGATGATGTAGCTCCGGAGTTTAGATTGATATTGGAGGGGACTTATTCATCATCTGTGAAACATCCTGTACAAGATACAAATGAGAAAAAGCTTTACTGGTGGGTGCAGAGGAAGAATTTTGATCGCCGCCTTGGTGAATTTTTGAAGAATTTGGAAGATTCATGGTTTGGTGCTTGGAAACTTATGCTACTGGGTGAATGGTCAGACCGTGAGCAGTTGGACTCGGTACTTGAGGATCTAGTGTGCAGTTTGAAATCCAAATGCAAAATGGAGATAGATGAAAGTGTTTTGAAAGTCATCCTTGGGGGTTCCAAATATGACTTTGAAGGAGGTCCATTTGTTACACAGCTTTGTCGTAAGAAAGGTTCCTACATTAATAAATTTGGATGTTTAGAGGAAGAAAAGTGTATGGCATCATGTAATGATTCTAGTGGTGGTGATAACTTGTCTGAGTCAGCCTACAAACTAGTATCAGAAGCAGTGAATGAGCTTAAAGGTTTACATTCTTGTGTGAATATAGAGCCAACAATATTAGTTCTGGACTACGAGGTGCAGATGCTTCCTTGGGAGAACTTACCAGTCCTTAGAAACCAGGAGGTGTATCGCATGCCCTCTGTTGGGAGCATTTTGGCAACAGCCAATAGAAACTACCAGAATCAAGATCAAGTTCAGAGTATAGCGACTTTATTTCCTTTGATTGATCCCTTGGATGCCTTTTATCTTCTGAATCCCAGTGGTGATCTAAATTATACACAAAATGAATTTGAGACATGGTTCAGAGATCAAAACTTGGAGGGGAAGGCTGGATCTGCACCTCCTGCTGAAGAATTGGCAGTAGCGTTGAGCAGCCATGATTTATTTTTGTATTTTGGCCATGGATGTGGGAAACAATATATTCCTAGACATGAGATTCAGAAACTGGAACACTGTGCCGCAACTCTCCTAATGGGATGTAGTAGTGGTTCTCTGAAACTGAATGGATGTTATGTTCCACAAGGTACTCCACTATCCTATTTGCTAGCCGGTTCTCCTGTCATTGTCGCCAATTTGTGGGATGTGACGGACAGGGATATTGACCGATTTGCAAAGTCCATGCTTGATTCATGGTTGAAAGCAAGGTCGAGTCCCTGTGTGGGTTGTGTCCAGAAATCGGACAAAAACCTGTCTTGTGAGCATAGGCCGACGGTTGGATCATTCATGAGTGAAGCACGTAAAACTTGCCAGTTACCTTTCTTGATCGGAGCAGCACCTGTATGCTATGGTGTCCCAACTGGCATATGGAAAAAAGAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

2140

Amino Acids

240.71

Weight (kDa)

6.3

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 9 - 283 1.6e-101 Separase-like, TPR repeats
TPR_ESP1_2nd PF25113 296 - 465 9.5e-36 Separase-like, TPR repeats
Separin_C PF03568 1660 - 2066 6e-97 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1332, 2207, 3315
AasI GACNNNNNNGTC 1 cut(s) 1210
AatII GACGTC 1 cut(s) 832
AbsI CCTCGAGG 1 cut(s) 374
Acc65I GGTACC 1 cut(s) 4801
AccB1I GGYRCC 2 cut(s) 1448, 4801
AccB7I CCANNNNNTGG 2 cut(s) 1638, 6174
AccBSI CCGCTC 1 cut(s) 604
AccI GTMKAC 2 cut(s) 1567, 1955
AccII CGCG 3 cut(s) 14, 198, 4703
AccIII TCCGGA 1 cut(s) 5137
AcoI YGGCCR 3 cut(s) 300, 3073, 5995
AcuI CTGAAG 3 cut(s) 1713, 3332, 5967
AcvI CACGTG 1 cut(s) 1294
AcyI GRCGYC 2 cut(s) 829, 4207
AdeI CACNNNGTG 2 cut(s) 1918, 4017
AfeI AGCGCT 2 cut(s) 1876, 2448
AflII CTTAAG 1 cut(s) 3488
AflIII ACRYGT 3 cut(s) 1694, 2541, 4780
AhlI ACTAGT 4 cut(s) 3061, 3275, 4513, 5590
AjuI GAANNNNNNNTTGG 2 cut(s) 5077, 5109
AloI GAACNNNNNNTCC 2 cut(s) 6096, 6128
Alw21I GWGCWC 4 cut(s) 1609, 2049, 2549, 4545
Alw26I GTCTC 7 cut(s) 11, 719, 1816, 2908, 4041, 4214, 5885
Alw44I GTGCAC 2 cut(s) 1605, 2045
AlwNI CAGNNNCTG 2 cut(s) 6043, 6374
Ama87I CYCGRG 3 cut(s) 374, 698, 4615
Aor13HI TCCGGA 1 cut(s) 5137
Aor51HI AGCGCT 2 cut(s) 1876, 2448
AoxI GGCC 9 cut(s) 3, 300, 378, 384, 3073, 4882, 4924, 5995, 6302
ApaI GGGCCC 1 cut(s) 388
ApaLI GTGCAC 2 cut(s) 1605, 2045
ArsI GACNNNNNNTTYG 6 cut(s) 464, 496, 1794, 1826, 4502, 4534
AseI ATTAAT 1 cut(s) 5499
Asp700I GAANNNNTTC 3 cut(s) 431, 838, 1641
Asp718I GGTACC 1 cut(s) 4801
AspLEI GCGC 8 cut(s) 200, 415, 509, 1877, 2449, 3123, 4703, 4705
AspS9I GGNCC 7 cut(s) 384, 385, 1988, 3264, 3921, 4925, 5456
AsuC2I CCSGG 3 cut(s) 64, 395, 3753
AsuII TTCGAA 1 cut(s) 4001
AsuNHI GCTAGC 1 cut(s) 6142
AvaI CYCGRG 3 cut(s) 374, 698, 4615
AvaII GGWCC 4 cut(s) 1988, 3264, 3921, 5456
BaeGI GKGCMC 5 cut(s) 373, 388, 1609, 2049, 4780
BaeI ACNNNNGTAYC 6 cut(s) 2377, 2410, 2763, 2796, 3960, 3993
BalI TGGCCA 1 cut(s) 5997
BamHI GGATCC 2 cut(s) 354, 2849
BanI GGYRCC 2 cut(s) 1448, 4801
BanII GRGCYC 3 cut(s) 388, 529, 1768
BarI GAAGNNNNNNTAC 2 cut(s) 3134, 3166
BauI CACGAG 3 cut(s) 1285, 1767, 1913
BbrPI CACGTG 1 cut(s) 1294
BbsI GAAGAC 3 cut(s) 137, 2117, 4127
Bbv12I GWGCWC 4 cut(s) 1609, 2049, 2549, 4545
BbvCI CCTCAGC 2 cut(s) 3387, 3393
BceAI ACGGC 5 cut(s) 290, 316, 3088, 3108, 4533
BcgI CGANNNNNNTGC 4 cut(s) 1276, 1310, 3447, 3481
BciVI GTATCC 2 cut(s) 2405, 3187
BclI TGATCA 2 cut(s) 1060, 3220
BcnI CCSGG 3 cut(s) 64, 395, 3753
BcoDI GTCTC 7 cut(s) 11, 719, 1816, 2908, 4041, 4214, 5885
BcuI ACTAGT 4 cut(s) 3061, 3275, 4513, 5590
BfmI CTRYAG 3 cut(s) 1681, 1959, 2535
BfoI RGCGCY 3 cut(s) 416, 1878, 2450
BfrI CTTAAG 1 cut(s) 3488
BfuI GTATCC 2 cut(s) 2405, 3187
BglI GCCNNNNNGGC 1 cut(s) 377
BglII AGATCT 1 cut(s) 1028
BmcAI AGTACT 2 cut(s) 1687, 3796
Bme18I GGWCC 4 cut(s) 1988, 3264, 3921, 5456
BmeT110I CYCGRG 3 cut(s) 374, 698, 4615
BmgT120I GGNCC 7 cut(s) 384, 385, 1988, 3264, 3921, 4925, 5456
BmrI ACTGGG 4 cut(s) 1698, 3198, 5324, 5850
BmtI GCTAGC 1 cut(s) 6146
BmuI ACTGGG 4 cut(s) 1698, 3198, 5324, 5850
BpiI GAAGAC 3 cut(s) 137, 2117, 4127
BplI GAGNNNNNCTC 6 cut(s) 694, 726, 735, 767, 3910, 3942
BpmI CTGGAG 2 cut(s) 2223, 2535
Bpu10I CCTNAGC 3 cut(s) 416, 3387, 3393
Bpu14I TTCGAA 1 cut(s) 4001
BpuEI CTTGAG 1 cut(s) 5375
BpuMI CCSGG 3 cut(s) 64, 395, 3753
BsaAI YACGTR 2 cut(s) 1294, 6335
BsaBI GATNNNNATC 4 cut(s) 2643, 3198, 3225, 3940
BsaHI GRCGYC 2 cut(s) 829, 4207
BsaI GGTCTC 3 cut(s) 11, 1816, 2908
BsaWI WCCGGW 2 cut(s) 357, 5137
BsaXI ACNNNNNCTCC 6 cut(s) 528, 558, 2725, 2755, 4409, 4439
Bse118I RCCGGY 2 cut(s) 460, 6146
Bse3DI GCAATG 3 cut(s) 1815, 3108, 4136
Bse8I GATNNNNATC 4 cut(s) 2643, 3198, 3225, 3940
BseAI TCCGGA 1 cut(s) 5137
BseJI GATNNNNATC 4 cut(s) 2643, 3198, 3225, 3940
BseMI GCAATG 3 cut(s) 1815, 3108, 4136
BsePI GCGCGC 1 cut(s) 4701
BseRI GAGGAG 6 cut(s) 23, 602, 605, 638, 1615, 2400
BseSI GKGCMC 5 cut(s) 373, 388, 1609, 2049, 4780
BsgI GTGCAG 7 cut(s) 1316, 2749, 3601, 5249, 5389, 5696, 5919
Bsh1236I CGCG 3 cut(s) 14, 198, 4703
BshFI GGCC 9 cut(s) 5, 302, 380, 386, 3075, 4884, 4926, 5997, 6304
BshNI GGYRCC 2 cut(s) 1448, 4801
BsiHKAI GWGCWC 4 cut(s) 1609, 2049, 2549, 4545
BsiHKCI CYCGRG 3 cut(s) 374, 698, 4615
BsiSI CCGG 8 cut(s) 63, 324, 358, 394, 461, 3753, 5138, 6147
BslFI GGGAC 6 cut(s) 840, 1705, 2151, 5176, 6234, 6374
BsmAI GTCTC 7 cut(s) 11, 719, 1816, 2908, 4041, 4214, 5885
BsmBI CGTCTC 1 cut(s) 4214
BsmFI GGGAC 6 cut(s) 840, 1705, 2151, 5176, 6234, 6374
BsmI GAATGC 3 cut(s) 929, 2922, 3874
BsnI GGCC 9 cut(s) 5, 302, 380, 386, 3075, 4884, 4926, 5997, 6304
Bso31I GGTCTC 3 cut(s) 11, 1816, 2908
BsoBI CYCGRG 3 cut(s) 374, 698, 4615
Bsp119I TTCGAA 1 cut(s) 4001
Bsp120I GGGCCC 1 cut(s) 384
Bsp1286I GDGCHC 9 cut(s) 373, 388, 529, 1609, 1768, 2049, 2549, 4545, 4780
Bsp13I TCCGGA 1 cut(s) 5137
Bsp1407I TGTACA 6 cut(s) 1701, 1865, 3160, 3693, 4425, 5191
Bsp19I CCATGG 4 cut(s) 297, 4140, 5105, 5998
BspANI GGCC 9 cut(s) 5, 302, 380, 386, 3075, 4884, 4926, 5997, 6304
BspEI TCCGGA 1 cut(s) 5137
BspFNI CGCG 3 cut(s) 14, 198, 4703
BspHI TCATGA 4 cut(s) 2647, 2781, 4267, 6321
BspMAI CTGCAG 1 cut(s) 1963
BspOI GCTAGC 1 cut(s) 6146
BspQI GCTCTTC 1 cut(s) 422
BspT104I TTCGAA 1 cut(s) 4001
BspT107I GGYRCC 2 cut(s) 1448, 4801
BspTI CTTAAG 1 cut(s) 3488
BspTNI GGTCTC 3 cut(s) 11, 1816, 2908
BsrBI CCGCTC 1 cut(s) 604
BsrDI GCAATG 3 cut(s) 1815, 3108, 4136
BsrFI RCCGGY 2 cut(s) 460, 6146
BsrGI TGTACA 6 cut(s) 1701, 1865, 3160, 3693, 4425, 5191
BssAI RCCGGY 2 cut(s) 460, 6146
BssHII GCGCGC 1 cut(s) 4701
BssNAI GTATAC 1 cut(s) 1568
BssNI GRCGYC 2 cut(s) 829, 4207
BssSI CACGAG 3 cut(s) 1285, 1767, 1913
Bst1107I GTATAC 1 cut(s) 1568
Bst2BI CACGAG 3 cut(s) 1285, 1767, 1913
Bst6I CTCTTC 2 cut(s) 422, 3351
BstACI GRCGYC 2 cut(s) 829, 4207
BstAFI CTTAAG 1 cut(s) 3488
BstAPI GCANNNNNTGC 3 cut(s) 765, 4784, 5942
BstAUI TGTACA 6 cut(s) 1701, 1865, 3160, 3693, 4425, 5191
BstBAI YACGTR 2 cut(s) 1294, 6335
BstBI TTCGAA 1 cut(s) 4001
BstDSI CCRYGG 5 cut(s) 12, 297, 4140, 5105, 5998
BstFNI CGCG 3 cut(s) 14, 198, 4703
BstH2I RGCGCY 3 cut(s) 416, 1878, 2450
BstHHI GCGC 8 cut(s) 200, 415, 509, 1877, 2449, 3123, 4703, 4705
BstMAI GTCTC 7 cut(s) 11, 719, 1816, 2908, 4041, 4214, 5885
BstNSI RCATGY 5 cut(s) 2545, 4104, 4784, 4979, 5734
BstSFI CTRYAG 3 cut(s) 1681, 1959, 2535
BstSLI GKGCMC 5 cut(s) 373, 388, 1609, 2049, 4780
BstUI CGCG 3 cut(s) 14, 198, 4703
BstV2I GAAGAC 3 cut(s) 137, 2117, 4127
BstX2I RGATCY 6 cut(s) 354, 1028, 2849, 3935, 5359, 5930
BstXI CCANNNNNNTGG 2 cut(s) 2247, 6005
BstYI RGATCY 6 cut(s) 354, 1028, 2849, 3935, 5359, 5930
BstZ17I GTATAC 1 cut(s) 1568
BsuI GTATCC 2 cut(s) 2405, 3187
BsuRI GGCC 9 cut(s) 5, 302, 380, 386, 3075, 4884, 4926, 5997, 6304
BtgI CCRYGG 5 cut(s) 12, 297, 4140, 5105, 5998
BtsI GCAGTG 2 cut(s) 2015, 5610
CaiI CAGNNNCTG 2 cut(s) 6043, 6374
CciI TCATGA 4 cut(s) 2647, 2781, 4267, 6321
CfoI GCGC 8 cut(s) 200, 415, 509, 1877, 2449, 3123, 4703, 4705
Cfr10I RCCGGY 2 cut(s) 460, 6146
Cfr13I GGNCC 7 cut(s) 384, 385, 1988, 3264, 3921, 4925, 5456
Cfr42I CCGCGG 1 cut(s) 15
CseI GACGC 1 cut(s) 4196
CspCI CAANNNNNGTGG 4 cut(s) 1980, 2015, 5104, 5139
DraI TTTAAA 4 cut(s) 1077, 1131, 1308, 4449
DraIII CACNNNGTG 2 cut(s) 1918, 4017
DrdI GACNNNNNNGTC 1 cut(s) 1210
DseDI GACNNNNNNGTC 1 cut(s) 1210
EaeI YGGCCR 3 cut(s) 300, 3073, 5995
Eam1104I CTCTTC 2 cut(s) 422, 3351
EarI CTCTTC 2 cut(s) 422, 3351
EciI GGCGGA 3 cut(s) 40, 127, 4338
Eco147I AGGCCT 2 cut(s) 380, 4884
Eco24I GRGCYC 3 cut(s) 388, 529, 1768
Eco31I GGTCTC 3 cut(s) 11, 1816, 2908
Eco32I GATATC 1 cut(s) 2497
Eco47I GGWCC 4 cut(s) 1988, 3264, 3921, 5456
Eco47III AGCGCT 2 cut(s) 1876, 2448
Eco57I CTGAAG 3 cut(s) 1713, 3332, 5967
Eco72I CACGTG 1 cut(s) 1294
Eco88I CYCGRG 3 cut(s) 374, 698, 4615
EcoO109I RGGNCCY 1 cut(s) 4925
EcoRI GAATTC 1 cut(s) 4871
EcoRV GATATC 1 cut(s) 2497
EcoT22I ATGCAT 5 cut(s) 1339, 2518, 2924, 4106, 4981
EcoT38I GRGCYC 3 cut(s) 388, 529, 1768
Esp3I CGTCTC 1 cut(s) 4214
FalI AAGNNNNNCTT 4 cut(s) 2964, 2996, 5409, 5441
FaqI GGGAC 6 cut(s) 840, 1705, 2151, 5176, 6234, 6374
FauI CCCGC 1 cut(s) 395
FauNDI CATATG 6 cut(s) 755, 813, 3097, 4084, 4433, 6401
FbaI TGATCA 2 cut(s) 1060, 3220
FblI GTMKAC 2 cut(s) 1567, 1955
FriOI GRGCYC 3 cut(s) 388, 529, 1768
GlaI GCGC 8 cut(s) 199, 414, 508, 1876, 2448, 3122, 4702, 4704
GsuI CTGGAG 2 cut(s) 2223, 2535
HaeII RGCGCY 3 cut(s) 416, 1878, 2450
HaeIII GGCC 9 cut(s) 5, 302, 380, 386, 3075, 4884, 4926, 5997, 6304
HapII CCGG 8 cut(s) 63, 324, 358, 394, 461, 3753, 5138, 6147
HgaI GACGC 1 cut(s) 4196
HhaI GCGC 8 cut(s) 200, 415, 509, 1877, 2449, 3123, 4703, 4705
Hin1I GRCGYC 2 cut(s) 829, 4207
Hin6I GCGC 8 cut(s) 198, 413, 507, 1875, 2447, 3121, 4701, 4703
HinP1I GCGC 8 cut(s) 198, 413, 507, 1875, 2447, 3121, 4701, 4703
HincII GTYRAC 2 cut(s) 1366, 4842
HindII GTYRAC 2 cut(s) 1366, 4842
HindIII AAGCTT 4 cut(s) 1586, 3436, 4689, 5213
HpaII CCGG 8 cut(s) 63, 324, 358, 394, 461, 3753, 5138, 6147
Hpy99I CGWCG 3 cut(s) 316, 834, 6310
HpyCH4IV ACGT 8 cut(s) 829, 1116, 1293, 1490, 1696, 1777, 2378, 6334
HpySE526I ACGT 8 cut(s) 829, 1116, 1293, 1490, 1696, 1777, 2378, 6334
Hsp92I GRCGYC 2 cut(s) 829, 4207
HspAI GCGC 8 cut(s) 198, 413, 507, 1875, 2447, 3121, 4701, 4703
Kpn2I TCCGGA 1 cut(s) 5137
KpnI GGTACC 1 cut(s) 4805
Ksp22I TGATCA 2 cut(s) 1060, 3220
KspI CCGCGG 1 cut(s) 15
LguI GCTCTTC 1 cut(s) 422
MaeII ACGT 8 cut(s) 829, 1116, 1293, 1490, 1696, 1777, 2378, 6334
MbiI CCGCTC 1 cut(s) 604
MfeI CAATTG 2 cut(s) 2858, 4695
MflI RGATCY 6 cut(s) 354, 1028, 2849, 3935, 5359, 5930
MhlI GDGCHC 9 cut(s) 373, 388, 529, 1609, 1768, 2049, 2549, 4545, 4780
MlsI TGGCCA 1 cut(s) 5997
MluNI TGGCCA 1 cut(s) 5997
MlyI GAGTC 9 cut(s) 287, 413, 902, 1868, 3019, 4847, 5339, 5585, 6256
MmeI TCCRAC 8 cut(s) 108, 885, 2090, 2899, 3757, 4777, 5322, 6292
Mox20I TGGCCA 1 cut(s) 5997
Mph1103I ATGCAT 5 cut(s) 1339, 2518, 2924, 4106, 4981
MroI TCCGGA 1 cut(s) 5137
MroXI GAANNNNTTC 3 cut(s) 431, 838, 1641
MscI TGGCCA 1 cut(s) 5997
MslI CAYNNNNRTG 9 cut(s) 396, 776, 895, 1668, 2802, 4087, 5631, 6003, 6376
Msp20I TGGCCA 1 cut(s) 5997
MspA1I CMGCKG 1 cut(s) 14
MspCI CTTAAG 1 cut(s) 3488
MspI CCGG 8 cut(s) 63, 324, 358, 394, 461, 3753, 5138, 6147
MunI CAATTG 2 cut(s) 2858, 4695
Mva1269I GAATGC 3 cut(s) 929, 2922, 3874
MvnI CGCG 3 cut(s) 14, 198, 4703
NciI CCSGG 3 cut(s) 64, 395, 3753
NcoI CCATGG 4 cut(s) 297, 4140, 5105, 5998
NdeI CATATG 6 cut(s) 755, 813, 3097, 4084, 4433, 6401
NheI GCTAGC 1 cut(s) 6142
NmuCI GTSAC 7 cut(s) 665, 873, 2233, 2630, 4091, 4864, 6181
NsiI ATGCAT 5 cut(s) 1339, 2518, 2924, 4106, 4981
NspI RCATGY 5 cut(s) 2545, 4104, 4784, 4979, 5734
NspV TTCGAA 1 cut(s) 4001
PaeI GCATGC 2 cut(s) 4979, 5734
PaeR7I CTCGAG 2 cut(s) 374, 4615
PagI TCATGA 4 cut(s) 2647, 2781, 4267, 6321
PasI CCCWGGG 1 cut(s) 4388
PauI GCGCGC 1 cut(s) 4701
PceI AGGCCT 2 cut(s) 380, 4884
PciI ACATGT 2 cut(s) 2541, 4780
PciSI GCTCTTC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 1774, 3014
PctI GAATGC 3 cut(s) 929, 2922, 3874
PdmI GAANNNNTTC 3 cut(s) 431, 838, 1641
PflMI CCANNNNNTGG 2 cut(s) 1638, 6174
PfoI TCCNGGA 1 cut(s) 946
PleI GAGTC 9 cut(s) 287, 412, 902, 1867, 3018, 4846, 5339, 5584, 6255
PmaCI CACGTG 1 cut(s) 1294
PmlI CACGTG 1 cut(s) 1294
PpsI GAGTC 9 cut(s) 287, 412, 902, 1867, 3018, 4846, 5339, 5584, 6255
Ppu21I YACGTR 2 cut(s) 1294, 6335
PscI ACATGT 2 cut(s) 2541, 4780
PshBI ATTAAT 1 cut(s) 5499
PsiI TTATAA 3 cut(s) 1332, 2207, 3315
PspCI CACGTG 1 cut(s) 1294
PspOMI GGGCCC 1 cut(s) 384
PspPI GGNCC 7 cut(s) 384, 385, 1988, 3264, 3921, 4925, 5456
PspXI VCTCGAGB 2 cut(s) 374, 4615
PsrI GAACNNNNNNTAC 4 cut(s) 5473, 5505, 6070, 6102
PstI CTGCAG 1 cut(s) 1963
PstNI CAGNNNCTG 2 cut(s) 6043, 6374
PsuI RGATCY 6 cut(s) 354, 1028, 2849, 3935, 5359, 5930
PteI GCGCGC 1 cut(s) 4701
RseI CAYNNNNRTG 9 cut(s) 396, 776, 895, 1668, 2802, 4087, 5631, 6003, 6376
SacII CCGCGG 1 cut(s) 15
SapI GCTCTTC 1 cut(s) 422
Sau96I GGNCC 7 cut(s) 384, 385, 1988, 3264, 3921, 4925, 5456
ScaI AGTACT 2 cut(s) 1687, 3796
SchI GAGTC 9 cut(s) 287, 413, 902, 1868, 3019, 4847, 5339, 5585, 6256
SduI GDGCHC 9 cut(s) 373, 388, 529, 1609, 1768, 2049, 2549, 4545, 4780
SfcI CTRYAG 3 cut(s) 1681, 1959, 2535
Sfr274I CTCGAG 2 cut(s) 374, 4615
Sfr303I CCGCGG 1 cut(s) 15
SfuI TTCGAA 1 cut(s) 4001
SgrBI CCGCGG 1 cut(s) 15
SinI GGWCC 4 cut(s) 1988, 3264, 3921, 5456
SlaI CTCGAG 2 cut(s) 374, 4615
SmiMI CAYNNNNRTG 9 cut(s) 396, 776, 895, 1668, 2802, 4087, 5631, 6003, 6376
SmlI CTYRAG 4 cut(s) 374, 3488, 4615, 5354
SmoI CTYRAG 4 cut(s) 374, 3488, 4615, 5354
SpeI ACTAGT 4 cut(s) 3061, 3275, 4513, 5590
SphI GCATGC 2 cut(s) 4979, 5734
SseBI AGGCCT 2 cut(s) 380, 4884
SspI AATATT 4 cut(s) 2268, 2605, 3542, 5655
StuI AGGCCT 2 cut(s) 380, 4884
TaiI ACGT 8 cut(s) 832, 1119, 1296, 1493, 1699, 1780, 2381, 6337
TaqII GACCGA 2 cut(s) 3457, 6216
TauI GCSGC 7 cut(s) 14, 251, 305, 607, 4595, 5253, 6059
TseFI GTSAC 7 cut(s) 665, 873, 2233, 2630, 4091, 4864, 6181
Tsp45I GTSAC 7 cut(s) 665, 873, 2233, 2630, 4091, 4864, 6181
TspGWI ACGGA 4 cut(s) 470, 892, 2489, 6200
Van91I CCANNNNNTGG 2 cut(s) 1638, 6174
Vha464I CTTAAG 1 cut(s) 3488
VneI GTGCAC 2 cut(s) 1605, 2045
VpaK11BI GGWCC 4 cut(s) 1988, 3264, 3921, 5456
VspI ATTAAT 1 cut(s) 5499
XbaI TCTAGA 2 cut(s) 3932, 4580
XceI RCATGY 5 cut(s) 2545, 4104, 4784, 4979, 5734
XcmI CCANNNNNNNNNTGG 5 cut(s) 3371, 3541, 4147, 5389, 6399
XhoI CTCGAG 2 cut(s) 374, 4615
XmiI GTMKAC 2 cut(s) 1567, 1955
XmnI GAANNNNTTC 3 cut(s) 431, 838, 1641
ZraI GACGTC 1 cut(s) 830
ZrmI AGTACT 2 cut(s) 1687, 3796
Zsp2I ATGCAT 5 cut(s) 1339, 2518, 2924, 4106, 4981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.