separase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Separin_C | PF03568 | 45 - 82 | 4e-06 | Separin, protease domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 291 |
| AcoI | YGGCCR | 2 cut(s) | 48, 223 |
| AcsI | RAATTY | 2 cut(s) | 256, 298 |
| AcuI | CTGAAG | 1 cut(s) | 195 |
| AfaI | GTAC | 1 cut(s) | 218 |
| AgsI | TTSAA | 2 cut(s) | 86, 196 |
| AhlI | ACTAGT | 1 cut(s) | 58 |
| AluBI | AGCT | 1 cut(s) | 82 |
| AluI | AGCT | 1 cut(s) | 82 |
| Alw21I | GWGCWC | 1 cut(s) | 166 |
| Alw44I | GTGCAC | 1 cut(s) | 162 |
| AoxI | GGCC | 2 cut(s) | 48, 223 |
| ApaLI | GTGCAC | 1 cut(s) | 162 |
| ApoI | RAATTY | 2 cut(s) | 256, 298 |
| BaeGI | GKGCMC | 1 cut(s) | 166 |
| BalI | TGGCCA | 2 cut(s) | 50, 225 |
| Bbv12I | GWGCWC | 1 cut(s) | 166 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 317, 351 |
| BcuI | ACTAGT | 1 cut(s) | 58 |
| BfaI | CTAG | 2 cut(s) | 59, 358 |
| BmsI | GCATC | 4 cut(s) | 14, 333, 340, 347 |
| BsaAI | YACGTR | 1 cut(s) | 11 |
| BsaJI | CCNNGG | 1 cut(s) | 226 |
| Bse1I | ACTGG | 2 cut(s) | 248, 269 |
| Bse3DI | GCAATG | 1 cut(s) | 78 |
| BseDI | CCNNGG | 1 cut(s) | 226 |
| BseGI | GGATG | 3 cut(s) | 164, 236, 287 |
| BseMI | GCAATG | 1 cut(s) | 78 |
| BseMII | CTCAG | 1 cut(s) | 33 |
| BseNI | ACTGG | 2 cut(s) | 248, 269 |
| BseSI | GKGCMC | 1 cut(s) | 166 |
| BsgI | GTGCAG | 1 cut(s) | 164 |
| BshFI | GGCC | 2 cut(s) | 50, 225 |
| BsiHKAI | GWGCWC | 1 cut(s) | 166 |
| BsnI | GGCC | 2 cut(s) | 50, 225 |
| Bsp1286I | GDGCHC | 1 cut(s) | 166 |
| Bsp1407I | TGTACA | 1 cut(s) | 216 |
| Bsp143I | GATC | 1 cut(s) | 205 |
| Bsp19I | CCATGG | 1 cut(s) | 226 |
| BspACI | CCGC | 1 cut(s) | 291 |
| BspANI | GGCC | 2 cut(s) | 50, 225 |
| BspCNI | CTCAG | 1 cut(s) | 34 |
| BsrDI | GCAATG | 1 cut(s) | 78 |
| BsrGI | TGTACA | 1 cut(s) | 216 |
| BsrI | ACTGG | 2 cut(s) | 248, 269 |
| BssECI | CCNNGG | 1 cut(s) | 226 |
| BssMI | GATC | 1 cut(s) | 205 |
| BssT1I | CCWWGG | 1 cut(s) | 226 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 170 |
| BstAUI | TGTACA | 1 cut(s) | 216 |
| BstBAI | YACGTR | 1 cut(s) | 11 |
| BstDEI | CTNAG | 1 cut(s) | 42 |
| BstDSI | CCRYGG | 1 cut(s) | 226 |
| BstF5I | GGATG | 3 cut(s) | 164, 236, 287 |
| BstKTI | GATC | 1 cut(s) | 208 |
| BstMBI | GATC | 1 cut(s) | 205 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 161, 170 |
| BstSLI | GKGCMC | 1 cut(s) | 166 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 233 |
| BsuRI | GGCC | 2 cut(s) | 50, 225 |
| BtgI | CCRYGG | 1 cut(s) | 226 |
| BtsCI | GGATG | 3 cut(s) | 164, 236, 287 |
| Csp6I | GTAC | 1 cut(s) | 217 |
| CviAII | CATG | 2 cut(s) | 203, 227 |
| CviJI | RGCY | 8 cut(s) | 50, 82, 115, 155, 201, 225, 277, 349 |
| CviKI_1 | RGCY | 8 cut(s) | 50, 82, 115, 155, 201, 225, 277, 349 |
| CviQI | GTAC | 1 cut(s) | 217 |
| DdeI | CTNAG | 1 cut(s) | 42 |
| DpnI | GATC | 1 cut(s) | 207 |
| DpnII | GATC | 1 cut(s) | 205 |
| EaeI | YGGCCR | 2 cut(s) | 48, 223 |
| EciI | GGCGGA | 1 cut(s) | 280 |
| Eco130I | CCWWGG | 1 cut(s) | 226 |
| Eco57I | CTGAAG | 1 cut(s) | 195 |
| EcoT14I | CCWWGG | 1 cut(s) | 226 |
| ErhI | CCWWGG | 1 cut(s) | 226 |
| FaeI | CATG | 2 cut(s) | 206, 230 |
| FaiI | YATR | 4 cut(s) | 110, 204, 215, 228 |
| FatI | CATG | 2 cut(s) | 202, 226 |
| FokI | GGATG | 3 cut(s) | 171, 243, 274 |
| FspBI | CTAG | 2 cut(s) | 59, 358 |
| HaeIII | GGCC | 2 cut(s) | 50, 225 |
| Hin1II | CATG | 2 cut(s) | 206, 230 |
| HinfI | GANTC | 2 cut(s) | 17, 237 |
| Hpy166II | GTNNAC | 2 cut(s) | 92, 164 |
| Hpy188I | TCNGA | 1 cut(s) | 43 |
| Hpy188III | TCNNGA | 2 cut(s) | 131, 353 |
| Hpy8I | GTNNAC | 2 cut(s) | 92, 164 |
| HpyAV | CCTTC | 2 cut(s) | 80, 145 |
| HpyCH4IV | ACGT | 1 cut(s) | 10 |
| HpyCH4V | TGCA | 3 cut(s) | 145, 164, 338 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 161, 170 |
| HpyF3I | CTNAG | 1 cut(s) | 42 |
| HpySE526I | ACGT | 1 cut(s) | 10 |
| Hsp92II | CATG | 2 cut(s) | 206, 230 |
| Kzo9I | GATC | 1 cut(s) | 205 |
| LpnPI | CCDG | 6 cut(s) | 116, 131, 141, 183, 229, 250 |
| LweI | GCATC | 4 cut(s) | 14, 333, 340, 347 |
| MaeI | CTAG | 2 cut(s) | 59, 358 |
| MaeII | ACGT | 1 cut(s) | 10 |
| MaeIII | GTNAC | 1 cut(s) | 353 |
| MalI | GATC | 1 cut(s) | 207 |
| MboI | GATC | 1 cut(s) | 205 |
| MboII | GAAGA | 3 cut(s) | 90, 188, 318 |
| MhlI | GDGCHC | 1 cut(s) | 166 |
| MlsI | TGGCCA | 2 cut(s) | 50, 225 |
| MluCI | AATT | 3 cut(s) | 179, 256, 298 |
| MluNI | TGGCCA | 2 cut(s) | 50, 225 |
| MnlI | CCTC | 3 cut(s) | 133, 177, 304 |
| Mox20I | TGGCCA | 2 cut(s) | 50, 225 |
| MscI | TGGCCA | 2 cut(s) | 50, 225 |
| MslI | CAYNNNNRTG | 1 cut(s) | 231 |
| Msp20I | TGGCCA | 2 cut(s) | 50, 225 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 161, 170 |
| NcoI | CCATGG | 1 cut(s) | 226 |
| NdeII | GATC | 1 cut(s) | 205 |
| NlaIII | CATG | 2 cut(s) | 206, 230 |
| NmuCI | GTSAC | 1 cut(s) | 353 |
| PfeI | GAWTC | 2 cut(s) | 17, 237 |
| Ppu21I | YACGTR | 1 cut(s) | 11 |
| RsaI | GTAC | 1 cut(s) | 218 |
| RsaNI | GTAC | 1 cut(s) | 217 |
| RseI | CAYNNNNRTG | 1 cut(s) | 231 |
| Sau3AI | GATC | 1 cut(s) | 205 |
| SduI | GDGCHC | 1 cut(s) | 166 |
| SetI | ASST | 5 cut(s) | 13, 84, 91, 144, 169 |
| SfaNI | GCATC | 4 cut(s) | 14, 333, 340, 347 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 231 |
| SpeI | ACTAGT | 1 cut(s) | 58 |
| Sse9I | AATT | 3 cut(s) | 179, 256, 298 |
| SsiI | CCGC | 1 cut(s) | 291 |
| SspI | AATATT | 1 cut(s) | 123 |
| SspMI | CTAG | 2 cut(s) | 59, 358 |
| StyI | CCWWGG | 1 cut(s) | 226 |
| TaiI | ACGT | 1 cut(s) | 13 |
| TaqI | TCGA | 3 cut(s) | 240, 261, 327 |
| TasI | AATT | 3 cut(s) | 179, 256, 298 |
| TatI | WGTACW | 1 cut(s) | 216 |
| TfiI | GAWTC | 2 cut(s) | 17, 237 |
| TseFI | GTSAC | 1 cut(s) | 353 |
| Tsp45I | GTSAC | 1 cut(s) | 353 |
| TspDTI | ATGAA | 3 cut(s) | 30, 89, 276 |
| VneI | GTGCAC | 1 cut(s) | 162 |
| XapI | RAATTY | 2 cut(s) | 256, 298 |
| XspI | CTAG | 2 cut(s) | 59, 358 |