RchiOBHm_Chr7g0223761

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000800
Physical Location & Seq
Reverse (-)
45623587 .. 45624430
844 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20035

Sequence Viewer

Length: 621 bp
ATGCCCTCTGTTGGTAGCATTTCGGCAACAACCAATAGAAACTACCACAATCAATATCATATTCAGAGTATTATGACTTCATTTCCTTTGATAGATCCCTTGGATGCCTTTTACCTTCTGAATCCCAGTGGTGATCTAAATTATACACAAAATGAATTTGAGACATGGTTTAGAGATCAAAACTTGGAGGGGAAGGCTGGATGTGCACCTCCTGCTGAAGAATTGGCAGTAGCGTTGAAAAGCCATGATCTATTTATGTACATTGGCCATGGATGTGGTATGTTTCGATTTTACATATTGCTTATGTGTTGCTGTATGTATACAGTTACTTCTGGCACTATTATTTTGCATAACAGGTTCCCCAATCATCCGTTTTTGAACTTTCATTTTGTGTACGTGCTGTGGTACAGGGCAACAATAATACAATATATTCCTAGACTTGAGATTCAGAGACTGGAACACTGTGCCTCAACTCTCCTGATGGGATGCAGTTGTGGTTCTCTGAAACTGAATGGATGTTATGTTCCACAAGGTACTCCACTATCCTATCTGCTGGCTGGTTCTCCTGTCATTCTCCTGATATTAACCGATTTGCAAATTCCATGCTTGATTCTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

206

Amino Acids

23.27

Weight (kDa)

6.2

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Separin_C PF03568 1 - 93 4.2e-18 Separin, protease domain
Separin_C PF03568 142 - 195 6.6e-16 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 320
AclWI GGATC 1 cut(s) 89
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 2 cut(s) 155, 597
AcuI CTGAAG 1 cut(s) 237
AfaI GTAC 4 cut(s) 260, 395, 407, 535
AfiI CCNNNNNNNGG 1 cut(s) 11
AgsI TTSAA 2 cut(s) 238, 379
AloI GAACNNNNNNTCC 2 cut(s) 507, 539
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 2 cut(s) 155, 445
Alw44I GTGCAC 1 cut(s) 204
AlwI GGATC 1 cut(s) 89
AlwNI CAGNNNCTG 1 cut(s) 454
AoxI GGCC 1 cut(s) 265
ApaLI GTGCAC 1 cut(s) 204
ApoI RAATTY 2 cut(s) 155, 597
AsuHPI GGTGA 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 208
BalI TGGCCA 1 cut(s) 267
BarI GAAGNNNNNNTAC 2 cut(s) 61, 93
Bbv12I GWGCWC 1 cut(s) 208
BccI CCATC 1 cut(s) 475
BcoDI GTCTC 2 cut(s) 155, 445
BfaI CTAG 1 cut(s) 435
BmiI GGNNCC 1 cut(s) 359
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 2 cut(s) 94, 476
BmuI ACTGGG 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 461
BsaAI YACGTR 1 cut(s) 397
BsaJI CCNNGG 2 cut(s) 99, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 11
Bse1I ACTGG 2 cut(s) 126, 459
BseDI CCNNGG 2 cut(s) 99, 268
BseGI GGATG 6 cut(s) 109, 206, 278, 367, 491, 521
BseLI CCNNNNNNNGG 1 cut(s) 11
BseNI ACTGG 2 cut(s) 126, 459
BseSI GKGCMC 1 cut(s) 208
BshFI GGCC 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 208
BslI CCNNNNNNNGG 1 cut(s) 11
BsmAI GTCTC 2 cut(s) 155, 445
BsnI GGCC 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 208
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 4 cut(s) 94, 133, 175, 247
Bsp19I CCATGG 1 cut(s) 268
BspANI GGCC 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 359
BspPI GGATC 1 cut(s) 89
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 2 cut(s) 126, 459
BssECI CCNNGG 2 cut(s) 99, 268
BssMI GATC 4 cut(s) 94, 133, 175, 247
BssNAI GTATAC 1 cut(s) 321
BssT1I CCWWGG 2 cut(s) 99, 268
Bst1107I GTATAC 1 cut(s) 321
Bst4CI ACNGT 2 cut(s) 325, 464
BstAPI GCANNNNNTGC 1 cut(s) 212
BstAUI TGTACA 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 397
BstC8I GCNNGC 1 cut(s) 555
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 6 cut(s) 109, 206, 278, 367, 491, 521
BstKTI GATC 4 cut(s) 97, 136, 178, 250
BstMAI GTCTC 2 cut(s) 155, 445
BstMBI GATC 4 cut(s) 94, 133, 175, 247
BstMWI GCNNNNNNNGC 2 cut(s) 203, 212
BstSLI GKGCMC 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 94
BstXI CCANNNNNNTGG 1 cut(s) 275
BstYI RGATCY 1 cut(s) 94
BstZ17I GTATAC 1 cut(s) 321
BsuRI GGCC 1 cut(s) 267
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 6 cut(s) 109, 206, 278, 367, 491, 521
BtsIMutI CAGTG 2 cut(s) 133, 460
Cac8I GCNNGC 1 cut(s) 555
CaiI CAGNNNCTG 1 cut(s) 454
Csp6I GTAC 4 cut(s) 259, 394, 406, 534
CviAII CATG 4 cut(s) 165, 245, 269, 603
CviJI RGCY 4 cut(s) 197, 243, 267, 557
CviKI_1 RGCY 4 cut(s) 197, 243, 267, 557
CviQI GTAC 4 cut(s) 259, 394, 406, 534
DpnI GATC 4 cut(s) 96, 135, 177, 249
DpnII GATC 4 cut(s) 94, 133, 175, 247
EaeI YGGCCR 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 99, 268
Eco57I CTGAAG 1 cut(s) 237
EcoT14I CCWWGG 2 cut(s) 99, 268
ErhI CCWWGG 2 cut(s) 99, 268
FaeI CATG 4 cut(s) 168, 248, 272, 606
FatI CATG 4 cut(s) 164, 244, 268, 602
FblI GTMKAC 1 cut(s) 320
FokI GGATG 6 cut(s) 116, 213, 285, 354, 498, 528
FspBI CTAG 1 cut(s) 435
HaeIII GGCC 1 cut(s) 267
Hin1II CATG 4 cut(s) 168, 248, 272, 606
HinfI GANTC 3 cut(s) 121, 445, 610
HphI GGTGA 1 cut(s) 143
Hpy166II GTNNAC 3 cut(s) 206, 321, 394
Hpy188I TCNGA 4 cut(s) 66, 120, 450, 504
Hpy188III TCNNGA 2 cut(s) 478, 577
Hpy8I GTNNAC 3 cut(s) 206, 321, 394
HpyAV CCTTC 2 cut(s) 125, 187
HpyCH4III ACNGT 2 cut(s) 325, 464
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 4 cut(s) 206, 349, 489, 595
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 212
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 4 cut(s) 168, 248, 272, 606
Kzo9I GATC 4 cut(s) 94, 133, 175, 247
LweI GCATC 2 cut(s) 94, 476
MaeI CTAG 1 cut(s) 435
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 1 cut(s) 325
MalI GATC 4 cut(s) 96, 135, 177, 249
MboI GATC 4 cut(s) 94, 133, 175, 247
MboII GAAGA 1 cut(s) 230
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 1 cut(s) 208
MlsI TGGCCA 1 cut(s) 267
MluCI AATT 4 cut(s) 139, 155, 221, 597
MluNI TGGCCA 1 cut(s) 267
MnlI CCTC 4 cut(s) 16, 181, 219, 478
Mox20I TGGCCA 1 cut(s) 267
MscI TGGCCA 1 cut(s) 267
MseI TTAA 1 cut(s) 584
MslI CAYNNNNRTG 1 cut(s) 273
Msp20I TGGCCA 1 cut(s) 267
MwoI GCNNNNNNNGC 2 cut(s) 203, 212
NcoI CCATGG 1 cut(s) 268
NdeII GATC 4 cut(s) 94, 133, 175, 247
NlaIII CATG 4 cut(s) 168, 248, 272, 606
NlaIV GGNNCC 1 cut(s) 359
PfeI GAWTC 3 cut(s) 121, 445, 610
Ppu21I YACGTR 1 cut(s) 397
PspN4I GGNNCC 1 cut(s) 359
PstNI CAGNNNCTG 1 cut(s) 454
PsuI RGATCY 1 cut(s) 94
RsaI GTAC 4 cut(s) 260, 395, 407, 535
RsaNI GTAC 4 cut(s) 259, 394, 406, 534
RseI CAYNNNNRTG 1 cut(s) 273
SaqAI TTAA 1 cut(s) 584
Sau3AI GATC 4 cut(s) 94, 133, 175, 247
SduI GDGCHC 1 cut(s) 208
SetI ASST 5 cut(s) 117, 211, 359, 399, 535
SfaNI GCATC 2 cut(s) 94, 476
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 4 cut(s) 139, 155, 221, 597
SspMI CTAG 1 cut(s) 435
StyI CCWWGG 2 cut(s) 99, 268
TaaI ACNGT 2 cut(s) 325, 464
TaiI ACGT 1 cut(s) 399
TaqI TCGA 1 cut(s) 286
TasI AATT 4 cut(s) 139, 155, 221, 597
TatI WGTACW 1 cut(s) 258
TfiI GAWTC 3 cut(s) 121, 445, 610
Tru1I TTAA 1 cut(s) 584
Tru9I TTAA 1 cut(s) 584
TscAI CASTG 2 cut(s) 133, 467
TspDTI ATGAA 3 cut(s) 69, 168, 374
TspGWI ACGGA 1 cut(s) 360
TspRI CASTG 2 cut(s) 133, 467
VneI GTGCAC 1 cut(s) 204
XapI RAATTY 2 cut(s) 155, 597
XmiI GTMKAC 1 cut(s) 320
XspI CTAG 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.