Rmu_sc0004923.1_g000010

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004923.1
OG0000800
Physical Location & Seq
Forward (+)
23222 .. 29276
6055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004923.1_g000010.1.cds

Sequence Viewer

Length: 672 bp
atgttagaggctcttaaggttttgaagagcttcaaggcgattgaattaggtacgaaatcggtgaatttgtattgtagatttgtgctaggtgtcgagcaaggaggctgcgataaaaagtttggggctttgctcggagaagtggtgtggacactagcgaagtatgtttcattaattcaaagcaaggaacacgaggtgtatgagtggctactttgtatggttgaggagttgggtcctcggtttaggtcagtgaatgccacctctggctggcgcaccggtgctgatcgtttaagctcgccctcgtctacgtcttcatggccttggatcatctatacgtcgaacatgaaaggagaatcaaagccggaaagccctcggtatttcggtggactcactgatcaagccatcaaagaccaaagacagagccagaggaggaggagaatagagaaaaaatatggagtagtggtcggtggattcactgatcaagccatcaatgcatcaagggccaaagatagagaggagaagaagaagaaaagaagaaaccgaggatgcagagggttaggctgcccattgctgaagaggacaaggatgaagaggaagaggaccatgaggaagaaagagggtttcgccagagatcgagaaagaaaatgggctcgatgggcgcggcggtggtgctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

223

Amino Acids

25.92

Weight (kDa)

10.41

Isoelectric Point (pI)

52.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 302
AccII CGCG 1 cut(s) 658
AciI CCGC 2 cut(s) 658, 661
AclWI GGATC 1 cut(s) 329
AcsI RAATTY 1 cut(s) 64
AcuI CTGAAG 1 cut(s) 590
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 1 cut(s) 52
AfiI CCNNNNNNNGG 1 cut(s) 264
AflII CTTAAG 1 cut(s) 14
AgeI ACCGGT 1 cut(s) 272
AgsI TTSAA 4 cut(s) 25, 34, 44, 176
AluBI AGCT 2 cut(s) 30, 291
AluI AGCT 2 cut(s) 30, 291
AlwI GGATC 1 cut(s) 329
AoxI GGCC 2 cut(s) 314, 498
ApeKI GCWGC 2 cut(s) 105, 558
ApoI RAATTY 1 cut(s) 64
AseI ATTAAT 1 cut(s) 170
AsiGI ACCGGT 1 cut(s) 272
Asp700I GAANNNNTTC 1 cut(s) 29
AspLEI GCGC 2 cut(s) 270, 658
AspS9I GGNCC 3 cut(s) 230, 498, 597
AsuHPI GGTGA 1 cut(s) 73
AvaII GGWCC 2 cut(s) 230, 597
BanII GRGCYC 1 cut(s) 649
BauI CACGAG 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 300
BbvI GCAGC 2 cut(s) 92, 545
BccI CCATC 3 cut(s) 407, 491, 645
BclI TGATCA 2 cut(s) 391, 475
BfaI CTAG 3 cut(s) 86, 152, 670
BfrI CTTAAG 1 cut(s) 14
BisI GCNGC 3 cut(s) 106, 559, 659
BlsI GCNGC 3 cut(s) 107, 560, 660
Bme18I GGWCC 2 cut(s) 230, 597
BmgT120I GGNCC 3 cut(s) 230, 498, 597
BmiI GGNNCC 1 cut(s) 231
BmsI GCATC 2 cut(s) 500, 533
BpiI GAAGAC 1 cut(s) 300
BsaJI CCNNGG 4 cut(s) 233, 317, 368, 538
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 4 cut(s) 126, 156, 444, 474
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse118I RCCGGY 1 cut(s) 272
Bse3DI GCAATG 1 cut(s) 563
BseDI CCNNGG 4 cut(s) 233, 317, 368, 538
BseGI GGATG 2 cut(s) 548, 588
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMI GCAATG 1 cut(s) 563
BseRI GAGGAG 5 cut(s) 236, 439, 442, 445, 527
BseXI GCAGC 2 cut(s) 92, 545
Bsh1236I CGCG 1 cut(s) 658
BshFI GGCC 2 cut(s) 316, 500
BshTI ACCGGT 1 cut(s) 272
BsiSI CCGG 2 cut(s) 273, 359
BslI CCNNNNNNNGG 1 cut(s) 264
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 2 cut(s) 316, 500
Bsp1286I GDGCHC 1 cut(s) 649
Bsp143I GATC 5 cut(s) 280, 321, 391, 475, 628
BspACI CCGC 2 cut(s) 658, 661
BspANI GGCC 2 cut(s) 316, 500
BspFNI CGCG 1 cut(s) 658
BspLI GGNNCC 1 cut(s) 231
BspPI GGATC 1 cut(s) 329
BspQI GCTCTTC 1 cut(s) 20
BspTI CTTAAG 1 cut(s) 14
BsrDI GCAATG 1 cut(s) 563
BsrFI RCCGGY 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 4 cut(s) 233, 317, 368, 538
BssMI GATC 5 cut(s) 280, 321, 391, 475, 628
BssSI CACGAG 1 cut(s) 188
BssT1I CCWWGG 1 cut(s) 317
Bst2BI CACGAG 1 cut(s) 188
Bst6I CTCTTC 4 cut(s) 20, 566, 581, 587
BstAFI CTTAAG 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 266, 293
BstF5I GGATG 2 cut(s) 548, 588
BstFNI CGCG 1 cut(s) 658
BstHHI GCGC 2 cut(s) 270, 658
BstKTI GATC 5 cut(s) 283, 324, 394, 478, 631
BstMBI GATC 5 cut(s) 280, 321, 391, 475, 628
BstMWI GCNNNNNNNGC 3 cut(s) 488, 497, 653
BstUI CGCG 1 cut(s) 658
BstV1I GCAGC 2 cut(s) 92, 545
BstV2I GAAGAC 1 cut(s) 300
BsuRI GGCC 2 cut(s) 316, 500
BtsCI GGATG 2 cut(s) 548, 588
BtsIMutI CAGTG 3 cut(s) 252, 387, 471
Cac8I GCNNGC 2 cut(s) 266, 293
CfoI GCGC 2 cut(s) 270, 658
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 3 cut(s) 230, 498, 597
Csp6I GTAC 1 cut(s) 51
CspAI ACCGGT 1 cut(s) 272
CviAII CATG 3 cut(s) 312, 340, 601
CviQI GTAC 1 cut(s) 51
DpnI GATC 5 cut(s) 282, 323, 393, 477, 630
DpnII GATC 5 cut(s) 280, 321, 391, 475, 628
DraIII CACNNNGTG 1 cut(s) 193
Eam1104I CTCTTC 4 cut(s) 20, 566, 581, 587
EarI CTCTTC 4 cut(s) 20, 566, 581, 587
Eco130I CCWWGG 1 cut(s) 317
Eco24I GRGCYC 1 cut(s) 649
Eco47I GGWCC 2 cut(s) 230, 597
Eco57I CTGAAG 1 cut(s) 590
EcoO109I RGGNCCY 1 cut(s) 230
EcoT14I CCWWGG 1 cut(s) 317
EcoT22I ATGCAT 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 649
ErhI CCWWGG 1 cut(s) 317
FaeI CATG 3 cut(s) 315, 343, 604
FaiI YATR 8 cut(s) 162, 198, 215, 313, 330, 341, 450, 602
FatI CATG 3 cut(s) 311, 339, 600
FbaI TGATCA 2 cut(s) 391, 475
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 3 cut(s) 106, 559, 659
FokI GGATG 2 cut(s) 555, 595
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 3 cut(s) 106, 559, 659
FspBI CTAG 3 cut(s) 86, 152, 670
GlaI GCGC 2 cut(s) 269, 657
GluI GCNGC 3 cut(s) 106, 559, 659
HaeIII GGCC 2 cut(s) 316, 500
HapII CCGG 2 cut(s) 273, 359
HhaI GCGC 2 cut(s) 270, 658
Hin1II CATG 3 cut(s) 315, 343, 604
Hin6I GCGC 2 cut(s) 268, 656
HinP1I GCGC 2 cut(s) 268, 656
HinfI GANTC 3 cut(s) 350, 384, 468
HpaII CCGG 2 cut(s) 273, 359
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 3 cut(s) 147, 303, 383
Hpy188I TCNGA 1 cut(s) 134
Hpy188III TCNNGA 1 cut(s) 632
Hpy8I GTNNAC 3 cut(s) 147, 303, 383
Hpy99I CGWCG 1 cut(s) 337
HpyCH4IV ACGT 2 cut(s) 305, 332
HpyCH4V TGCA 2 cut(s) 491, 546
HpyF10VI GCNNNNNNNGC 3 cut(s) 488, 497, 653
HpySE526I ACGT 2 cut(s) 305, 332
Hsp92II CATG 3 cut(s) 315, 343, 604
HspAI GCGC 2 cut(s) 268, 656
Ksp22I TGATCA 2 cut(s) 391, 475
Kzo9I GATC 5 cut(s) 280, 321, 391, 475, 628
LguI GCTCTTC 1 cut(s) 20
LpnPI CCDG 6 cut(s) 246, 250, 286, 372, 434, 637
Lsp1109I GCAGC 2 cut(s) 92, 545
LweI GCATC 2 cut(s) 500, 533
MaeI CTAG 3 cut(s) 86, 152, 670
MaeII ACGT 2 cut(s) 305, 332
MalI GATC 5 cut(s) 282, 323, 393, 477, 630
MboI GATC 5 cut(s) 280, 321, 391, 475, 628
MhlI GDGCHC 1 cut(s) 649
MluCI AATT 3 cut(s) 44, 64, 171
MlyI GAGTC 1 cut(s) 378
Mph1103I ATGCAT 1 cut(s) 493
MroXI GAANNNNTTC 1 cut(s) 29
MseI TTAA 3 cut(s) 15, 170, 287
MspCI CTTAAG 1 cut(s) 14
MspI CCGG 2 cut(s) 273, 359
Mva1269I GAATGC 1 cut(s) 256
MvnI CGCG 1 cut(s) 658
MwoI GCNNNNNNNGC 3 cut(s) 488, 497, 653
NdeII GATC 5 cut(s) 280, 321, 391, 475, 628
NlaIII CATG 3 cut(s) 315, 343, 604
NlaIV GGNNCC 1 cut(s) 231
NsiI ATGCAT 1 cut(s) 493
PciSI GCTCTTC 1 cut(s) 20
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 29
PfeI GAWTC 2 cut(s) 350, 468
PinAI ACCGGT 1 cut(s) 272
PkrI GCNGC 3 cut(s) 107, 560, 660
PleI GAGTC 1 cut(s) 378
PpsI GAGTC 1 cut(s) 378
PpuMI RGGWCCY 1 cut(s) 230
PshBI ATTAAT 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 230
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 3 cut(s) 230, 498, 597
PspPPI RGGWCCY 1 cut(s) 230
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 3 cut(s) 15, 170, 287
SatI GCNGC 3 cut(s) 106, 559, 659
Sau3AI GATC 5 cut(s) 280, 321, 391, 475, 628
Sau96I GGNCC 3 cut(s) 230, 498, 597
SchI GAGTC 1 cut(s) 378
SduI GDGCHC 1 cut(s) 649
SfaNI GCATC 2 cut(s) 500, 533
SgrAI CRCCGGYG 1 cut(s) 272
SinI GGWCC 2 cut(s) 230, 597
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
Sse9I AATT 3 cut(s) 44, 64, 171
SsiI CCGC 2 cut(s) 658, 661
SspMI CTAG 3 cut(s) 86, 152, 670
StyI CCWWGG 1 cut(s) 317
TaiI ACGT 2 cut(s) 308, 335
TaqI TCGA 4 cut(s) 93, 335, 631, 649
TasI AATT 3 cut(s) 44, 64, 171
TauI GCSGC 1 cut(s) 661
TfiI GAWTC 2 cut(s) 350, 468
Tru1I TTAA 3 cut(s) 15, 170, 287
Tru9I TTAA 3 cut(s) 15, 170, 287
TscAI CASTG 3 cut(s) 252, 394, 478
TseI GCWGC 2 cut(s) 105, 558
TspDTI ATGAA 4 cut(s) 156, 300, 356, 599
TspRI CASTG 3 cut(s) 252, 394, 478
Vha464I CTTAAG 1 cut(s) 14
VpaK11BI GGWCC 2 cut(s) 230, 597
VspI ATTAAT 1 cut(s) 170
XapI RAATTY 1 cut(s) 64
XmiI GTMKAC 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 29
XspI CTAG 3 cut(s) 86, 152, 670
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.