FvH4_1g08540

Cupin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000361
Physical Location & Seq
Forward (+)
4525527 .. 4528416
2890 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g08540.t1

Sequence Viewer

Length: 1170 bp
ATGATTTTCCCTATCTTCTTCACATTTTTGCTCCTCCTCTCCTCATCCCATGCTTCTGTGCAAGACTTCTGCGTAGCAGACTTGACAGCTCCAGAAGGCCCTGCAGGCTACTCCTGCAAGAAGCCTGCAAAAGTTACCGTAGATGACTTTGTCTTCTCTGGTCTAGGCATTGCTGGTAACACCACCAATATCATCAAAGCTGCAGTCACACCTGCATTTGCTGCTCAATTCCCTGGTGTTAATGGCCTCGGCATTTCGCTGGCTCGTTTAGACTTGGCTGTTGATGGAGTCATCCCCTTTCACACGCACCCCGGAGCTTCAGAAGTCCTGATTGTTGTGGAAGGCACAATCTGTGCAGGCTTTGTTTCCTCGGATAACACAGTTTATCTACAAACTCTTGAGACGGGTGACAGTATGGTCTTCCCTCAAGGTTTGTTGCACTTCCAAGTCAATGGAGGTGATACTCCAGCCCTTGCCTTTGTTAGCTTCAGTAGTGCTAGCCCCGGTCTGCAGATTCTGGACTTTGCATTGTTTAAAAACGACTTACCAACCTCATTGATAGCTCAAACTACATTCCTCGACGTTGCTCAGATAAAGAAACTCAAGGGTGTTCTTGGTGACTACACAGCTCCTCAAGGCCCCGCAGGCTACTCCTGCAAAGATCCCGCAAATGTCACGGTAGACGATTTCGTCTATTCTGCCCTAGGAGTTCCCGGTAACACCTCAAATATGATCAAAACCGGAATCACCACCGCATTTTCTCCTCAATTCCCTGGTTTGAATGGCCTTGGCGTTGCACTAGCTCGCGCAGACTTGGCTGTTGGTGGAGTTGTCCCTATGCACACACACCATGGAGCTTCAGAAATTGTACTTATCATTGAAGGAACTATAACCGCCGGGTTCATTTCCTCAGATAACGAAGTTTATGTAAAAACTCTGAAGAAGGGTGACATCATGGTTTTCCCTCAAGGACTGTTGCACTTCCAAGTGAATGTTGGTGATACTCCAGCTCTTGAGTTTGTTAGCTTCAGCAGTGATAACCCCGGTCTGCAGATTCTCTATACCGCACTATTTCAAAACGATTTACCTACTGACCTAATCGCGCGATCTACTTTCCTCGACACTGCTGAGATAAAGAAACTCAAGGCTCTTCTTGGTGGTACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

390

Amino Acids

40.75

Weight (kDa)

4.8

Isoelectric Point (pI)

24.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 54 - 198 1.5e-36 Cupin
Cupin_2 PF07883 91 - 161 2.5e-08 Cupin domain
Cupin_1 PF00190 235 - 378 7.8e-37 Cupin
Cupin_2 PF07883 270 - 337 7.9e-09 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000361)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20630
fragaria_vesca FvH4_1g08531 FvH4_1g08540 FvH4_1g08541 FvH4_1g18170 FvH4_1g18630 FvH4_2g06460 FvH4_6g36650
malus_domestica MD02G1089800.v1.1 MD15G1216400.v1.1 MD15G1216500.v1.1
prunus_persica Prupe.7G200100_v2.0.a1 Prupe.7G200200_v2.0.a1 Prupe.7G200300_v2.0.a1
pyrus_communis pycom02g07170 pycom15g19190
rosa_chinensis RchiOBHm_Chr2g0094741 RchiOBHm_Chr2g0094751 RchiOBHm_Chr2g0094761 RchiOBHm_Chr2g0094781 RchiOBHm_Chr2g0094791 RchiOBHm_Chr2g0095301 RchiOBHm_Chr2g0108661 RchiOBHm_Chr2g0109441 RchiOBHm_Chr2g0149111
rosa_laevigata RLG00000002180 RLG00000003674 RLG00000016514 RLG00000016515 RLG00000016516 RLG00000016517 RLG00000016521 RLG00000017684 RLG00000017738 RLG00000020370
rosa_multiflora Rmu_co8274667.1_g000001 Rmu_co8326765.1_g000001 Rmu_co8421367.1_g000001 Rmu_sc0000332.1_g000020 Rmu_sc0001588.1_g000008 Rmu_sc0002105.1_g000003 Rmu_sc0002345.1_g000005 Rmu_sc0004359.1_g000003 Rmu_sc0004359.1_g000007 Rmu_sc0004359.1_g000008 Rmu_sc0004359.1_g000010 Rmu_sc0004359.1_g000012 Rmu_sc0004359.1_g000015 Rmu_sc0006422.1_g000015 Rmu_sc0006422.1_g000017 Rmu_sc0006422.1_g000019 Rmu_sc0006422.1_g000021 Rmu_sc0006422.1_g000022 Rmu_sc0012763.1_g000007 Rmu_sc0022670.1_g000002 Rmu_sc0025419.1_g000001
rosa_roxburghii Rroxscaffold_2G00098450 Rroxscaffold_2G00134240 Rroxscaffold_2G00146910 Rroxscaffold_2G00146920 Rroxscaffold_2G00146930 Rroxscaffold_2G00146940 Rroxscaffold_3G00238780
rosa_rugosa Rorug02G0046400.1 Rorug02G0046500.1 Rorug02G0046600.1 Rorug02G0046700.1 Rorug02G0046900 Rorug02G0049100 Rorug02G0155100 Rorug02G0159900 Rorug02G0160200 Rorug02G0409200 Rorug07G0200300
rosa_samantha Rh2AG093700 Rh2AG093800 Rh2AG093900 Rh2AG094000 Rh2AG094100 Rh2AG094400 Rh2AG097200 Rh2AG204600 Rh2AG212100 Rh2AG469000 Rh2BG094200 Rh2BG094400 Rh2BG094700 Rh2BG095000 Rh2BG222500 Rh2BG481500 Rh2DG093300 Rh2DG093400 Rh2DG093500 Rh2DG093700 Rh2DG093800 Rh2DG098300 Rh2DG217700 Rh2DG490400 Rh7AG339600 Rh7CG357600 Rh7DG338300
rosa_wichuraiana Rw2G007340 Rw2G016310 Rw2G038180 Rw7G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 220
AasI GACNNNNNNGTC 2 cut(s) 416, 689
Acc36I ACCTGC 1 cut(s) 220
AccI GTMKAC 1 cut(s) 681
AccII CGCG 3 cut(s) 807, 1103, 1105
AciI CCGC 5 cut(s) 642, 666, 753, 894, 1065
AclWI GGATC 1 cut(s) 656
AcuI CTGAAG 5 cut(s) 303, 472, 843, 959, 1012
AfaI GTAC 2 cut(s) 870, 1162
AgsI TTSAA 3 cut(s) 781, 881, 1076
AjnI CCWGG 2 cut(s) 232, 772
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 1 cut(s) 656
AlwNI CAGNNNCTG 1 cut(s) 517
AoxI GGCC 4 cut(s) 97, 244, 637, 784
ApeKI GCWGC 2 cut(s) 200, 221
ArsI GACNNNNNNTTYG 2 cut(s) 189, 221
AspA2I CCTAGG 1 cut(s) 703
AspLEI GCGC 2 cut(s) 809, 1105
AspS9I GGNCC 2 cut(s) 98, 638
AsuC2I CCSGG 5 cut(s) 312, 504, 714, 898, 1044
AsuHPI GGTGA 6 cut(s) 419, 470, 629, 739, 959, 1010
AsuNHI GCTAGC 1 cut(s) 497
AvrII CCTAGG 1 cut(s) 703
BbsI GAAGAC 2 cut(s) 145, 412
BbvI GCAGC 2 cut(s) 187, 208
BccI CCATC 1 cut(s) 278
BciT130I CCWGG 2 cut(s) 234, 774
BclI TGATCA 1 cut(s) 732
BcnI CCSGG 5 cut(s) 312, 504, 714, 898, 1044
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 4 cut(s) 164, 498, 704, 800
BfmI CTRYAG 4 cut(s) 102, 201, 509, 1049
BfuAI ACCTGC 1 cut(s) 220
BglI GCCNNNNNGGC 2 cut(s) 105, 645
BisI GCNGC 2 cut(s) 201, 222
BlnI CCTAGG 1 cut(s) 703
BlsI GCNGC 2 cut(s) 202, 223
Bme1390I CCNGG 7 cut(s) 234, 312, 504, 714, 774, 898, 1044
BmgT120I GGNCC 2 cut(s) 98, 638
BmiI GGNNCC 1 cut(s) 640
BmrFI CCNGG 7 cut(s) 234, 312, 504, 714, 774, 898, 1044
BmtI GCTAGC 1 cut(s) 501
BpiI GAAGAC 2 cut(s) 145, 412
BpmI CTGGAG 3 cut(s) 75, 450, 990
BpuEI CTTGAG 7 cut(s) 411, 419, 587, 618, 951, 1034, 1127
BpuMI CCSGG 5 cut(s) 312, 504, 714, 898, 1044
BsaWI WCCGGW 1 cut(s) 740
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 2 cut(s) 234, 774
BseGI GGATG 2 cut(s) 44, 291
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 3 cut(s) 602, 924, 1119
BseRI GAGGAG 5 cut(s) 23, 26, 31, 621, 753
BseXI GCAGC 2 cut(s) 187, 208
BsgI GTGCAG 1 cut(s) 375
Bsh1236I CGCG 3 cut(s) 807, 1103, 1105
BshFI GGCC 4 cut(s) 99, 246, 639, 786
BsiSI CCGG 6 cut(s) 312, 504, 714, 741, 897, 1044
BslFI GGGAC 1 cut(s) 818
BsmAI GTCTC 1 cut(s) 395
BsmBI CGTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 818
BsnI GGCC 4 cut(s) 99, 246, 639, 786
Bsp143I GATC 3 cut(s) 661, 732, 1106
Bsp19I CCATGG 1 cut(s) 850
BspACI CCGC 5 cut(s) 642, 666, 753, 894, 1065
BspANI GGCC 4 cut(s) 99, 246, 639, 786
BspCNI CTCAG 3 cut(s) 601, 923, 1120
BspFNI CGCG 3 cut(s) 807, 1103, 1105
BspLI GGNNCC 1 cut(s) 640
BspMAI CTGCAG 4 cut(s) 106, 205, 513, 1053
BspMI ACCTGC 1 cut(s) 220
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 1 cut(s) 656
BspQI GCTCTTC 1 cut(s) 1155
BsrDI GCAATG 1 cut(s) 168
BssMI GATC 3 cut(s) 661, 732, 1106
BssT1I CCWWGG 3 cut(s) 703, 787, 850
Bst2UI CCWGG 2 cut(s) 234, 774
Bst4CI ACNGT 5 cut(s) 139, 382, 413, 679, 975
Bst6I CTCTTC 1 cut(s) 1155
BstAPI GCANNNNNTGC 1 cut(s) 221
BstC8I GCNNGC 7 cut(s) 106, 126, 261, 358, 499, 646, 805
BstDEI CTNAG 3 cut(s) 588, 910, 1128
BstDSI CCRYGG 1 cut(s) 850
BstF5I GGATG 2 cut(s) 44, 291
BstFNI CGCG 3 cut(s) 807, 1103, 1105
BstHHI GCGC 2 cut(s) 809, 1105
BstKTI GATC 3 cut(s) 664, 735, 1109
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 3 cut(s) 661, 732, 1106
BstMWI GCNNNNNNNGC 6 cut(s) 105, 114, 221, 645, 654, 815
BstNI CCWGG 2 cut(s) 234, 774
BstSCI CCNGG 7 cut(s) 232, 310, 502, 712, 772, 896, 1042
BstSFI CTRYAG 4 cut(s) 102, 201, 509, 1049
BstUI CGCG 3 cut(s) 807, 1103, 1105
BstV1I GCAGC 2 cut(s) 187, 208
BstV2I GAAGAC 2 cut(s) 145, 412
BstX2I RGATCY 1 cut(s) 661
BstXI CCANNNNNNTGG 1 cut(s) 452
BstYI RGATCY 1 cut(s) 661
BsuRI GGCC 4 cut(s) 99, 246, 639, 786
BtgI CCRYGG 1 cut(s) 850
BtsCI GGATG 2 cut(s) 44, 291
BtsI GCAGTG 2 cut(s) 1039, 1122
BtsIMutI CAGTG 2 cut(s) 1039, 1122
BveI ACCTGC 1 cut(s) 220
Cac8I GCNNGC 7 cut(s) 106, 126, 261, 358, 499, 646, 805
CaiI CAGNNNCTG 1 cut(s) 517
CfoI GCGC 2 cut(s) 809, 1105
Cfr13I GGNCC 2 cut(s) 98, 638
Csp6I GTAC 2 cut(s) 869, 1161
CviAII CATG 3 cut(s) 50, 851, 955
CviQI GTAC 2 cut(s) 869, 1161
DdeI CTNAG 3 cut(s) 588, 910, 1128
DpnI GATC 3 cut(s) 663, 734, 1108
DpnII GATC 3 cut(s) 661, 732, 1106
DraI TTTAAA 1 cut(s) 535
DrdI GACNNNNNNGTC 2 cut(s) 416, 689
DseDI GACNNNNNNGTC 2 cut(s) 416, 689
Eam1104I CTCTTC 1 cut(s) 1155
EarI CTCTTC 1 cut(s) 1155
Eco130I CCWWGG 3 cut(s) 703, 787, 850
Eco57I CTGAAG 5 cut(s) 303, 472, 843, 959, 1012
EcoO109I RGGNCCY 2 cut(s) 98, 638
EcoRII CCWGG 2 cut(s) 232, 772
EcoT14I CCWWGG 3 cut(s) 703, 787, 850
ErhI CCWWGG 3 cut(s) 703, 787, 850
Esp3I CGTCTC 1 cut(s) 395
FaeI CATG 3 cut(s) 53, 854, 958
FaiI YATR 9 cut(s) 51, 416, 731, 839, 852, 890, 927, 956, 1062
FaqI GGGAC 1 cut(s) 818
FatI CATG 3 cut(s) 49, 850, 954
FauI CCCGC 2 cut(s) 649, 673
FbaI TGATCA 1 cut(s) 732
FblI GTMKAC 1 cut(s) 681
Fnu4HI GCNGC 2 cut(s) 201, 222
FokI GGATG 2 cut(s) 31, 278
Fsp4HI GCNGC 2 cut(s) 201, 222
FspBI CTAG 4 cut(s) 164, 498, 704, 800
GlaI GCGC 2 cut(s) 808, 1104
GluI GCNGC 2 cut(s) 201, 222
GsuI CTGGAG 3 cut(s) 75, 450, 990
HaeIII GGCC 4 cut(s) 99, 246, 639, 786
HapII CCGG 6 cut(s) 312, 504, 714, 741, 897, 1044
HhaI GCGC 2 cut(s) 809, 1105
Hin1II CATG 3 cut(s) 53, 854, 958
Hin6I GCGC 2 cut(s) 807, 1103
HinP1I GCGC 2 cut(s) 807, 1103
HinfI GANTC 4 cut(s) 288, 514, 744, 1054
HpaII CCGG 6 cut(s) 312, 504, 714, 741, 897, 1044
HphI GGTGA 6 cut(s) 419, 470, 629, 739, 959, 1010
Hpy166II GTNNAC 1 cut(s) 682
Hpy188I TCNGA 6 cut(s) 322, 373, 591, 862, 913, 939
Hpy188III TCNNGA 5 cut(s) 92, 328, 398, 518, 1013
Hpy8I GTNNAC 1 cut(s) 682
Hpy99I CGWCG 1 cut(s) 584
HpyAV CCTTC 4 cut(s) 89, 335, 875, 937
HpyCH4III ACNGT 5 cut(s) 139, 382, 413, 679, 975
HpyCH4IV ACGT 1 cut(s) 582
HpyF10VI GCNNNNNNNGC 6 cut(s) 105, 114, 221, 645, 654, 815
HpyF3I CTNAG 3 cut(s) 588, 910, 1128
HpySE526I ACGT 1 cut(s) 582
Hsp92II CATG 3 cut(s) 53, 854, 958
HspAI GCGC 2 cut(s) 807, 1103
Ksp22I TGATCA 1 cut(s) 732
Kzo9I GATC 3 cut(s) 661, 732, 1106
LguI GCTCTTC 1 cut(s) 1155
LmnI GCTCC 5 cut(s) 36, 94, 314, 634, 854
Lsp1109I GCAGC 2 cut(s) 187, 208
MaeI CTAG 4 cut(s) 164, 498, 704, 800
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 8 cut(s) 133, 176, 205, 407, 617, 673, 716, 947
MalI GATC 3 cut(s) 663, 734, 1108
MboI GATC 3 cut(s) 661, 732, 1106
MboII GAAGA 6 cut(s) 7, 10, 145, 412, 952, 1142
MflI RGATCY 1 cut(s) 661
MluCI AATT 4 cut(s) 227, 767, 864, 1165
MlyI GAGTC 1 cut(s) 297
MseI TTAA 3 cut(s) 240, 534, 1168
MspI CCGG 6 cut(s) 312, 504, 714, 741, 897, 1044
MspR9I CCNGG 7 cut(s) 234, 312, 504, 714, 774, 898, 1044
MvaI CCWGG 2 cut(s) 234, 774
MvnI CGCG 3 cut(s) 807, 1103, 1105
MwoI GCNNNNNNNGC 6 cut(s) 105, 114, 221, 645, 654, 815
NciI CCSGG 5 cut(s) 312, 504, 714, 898, 1044
NcoI CCATGG 1 cut(s) 850
NdeII GATC 3 cut(s) 661, 732, 1106
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 3 cut(s) 53, 854, 958
NlaIV GGNNCC 1 cut(s) 640
NmeAIII GCCGAG 1 cut(s) 228
NmuCI GTSAC 5 cut(s) 205, 407, 617, 673, 947
PaqCI CACCTGC 1 cut(s) 220
PciSI GCTCTTC 1 cut(s) 1155
PfeI GAWTC 3 cut(s) 514, 744, 1054
PflFI GACNNNGTC 1 cut(s) 149
PkrI GCNGC 2 cut(s) 202, 223
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
Psp6I CCWGG 2 cut(s) 232, 772
PspGI CCWGG 2 cut(s) 232, 772
PspN4I GGNNCC 1 cut(s) 640
PspPI GGNCC 2 cut(s) 98, 638
PstI CTGCAG 4 cut(s) 106, 205, 513, 1053
PstNI CAGNNNCTG 1 cut(s) 517
PsuI RGATCY 1 cut(s) 661
PsyI GACNNNGTC 1 cut(s) 149
RsaI GTAC 2 cut(s) 870, 1162
RsaNI GTAC 2 cut(s) 869, 1161
SapI GCTCTTC 1 cut(s) 1155
SaqAI TTAA 3 cut(s) 240, 534, 1168
SatI GCNGC 2 cut(s) 201, 222
Sau3AI GATC 3 cut(s) 661, 732, 1106
Sau96I GGNCC 2 cut(s) 98, 638
SbfI CCTGCAGG 1 cut(s) 106
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 7 cut(s) 234, 312, 504, 714, 774, 898, 1044
SdaI CCTGCAGG 1 cut(s) 106
SfcI CTRYAG 4 cut(s) 102, 201, 509, 1049
SmlI CTYRAG 7 cut(s) 398, 426, 602, 633, 966, 1013, 1142
SmoI CTYRAG 7 cut(s) 398, 426, 602, 633, 966, 1013, 1142
Sse8387I CCTGCAGG 1 cut(s) 106
Sse9I AATT 4 cut(s) 227, 767, 864, 1165
SsiI CCGC 5 cut(s) 642, 666, 753, 894, 1065
SspMI CTAG 4 cut(s) 164, 498, 704, 800
StyD4I CCNGG 7 cut(s) 232, 310, 502, 712, 772, 896, 1042
StyI CCWWGG 3 cut(s) 703, 787, 850
TaaI ACNGT 5 cut(s) 139, 382, 413, 679, 975
TaiI ACGT 1 cut(s) 585
TaqI TCGA 2 cut(s) 579, 1119
TasI AATT 4 cut(s) 227, 767, 864, 1165
TatI WGTACW 1 cut(s) 868
TfiI GAWTC 3 cut(s) 514, 744, 1054
Tru1I TTAA 3 cut(s) 240, 534, 1168
Tru9I TTAA 3 cut(s) 240, 534, 1168
TscAI CASTG 2 cut(s) 1039, 1129
TseFI GTSAC 5 cut(s) 205, 407, 617, 673, 947
TseI GCWGC 2 cut(s) 200, 221
Tsp45I GTSAC 5 cut(s) 205, 407, 617, 673, 947
TspDTI ATGAA 1 cut(s) 892
TspRI CASTG 2 cut(s) 1039, 1129
Tth111I GACNNNGTC 1 cut(s) 149
XcmI CCANNNNNNNNNTGG 1 cut(s) 992
XmaJI CCTAGG 1 cut(s) 703
XmiI GTMKAC 1 cut(s) 681
XspI CTAG 4 cut(s) 164, 498, 704, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.