Rroxscaffold_2G00146920

Auxin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000361
Physical Location & Seq
Reverse (-)
84807113 .. 84808030
918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146920.1

Sequence Viewer

Length: 678 bp
ATGCTTCAACAAGCACATACCTATCGCTCAAAGTTGATCATGATGAGGACGTCTTTCCCTATTTTCTTCGTGTTATCTCTCATTCTCTCTTCCTCCTATGCTGCTGTGCAAGACTTCTGTGTTGCAGACTACACAGCTCCTCAAGGCCCTGCAGGTTACTCTTGCAAAAATCCTGCAAATGTTACGGTGGATGATTTCGTCTACTCTGCCCTAGGAGTTCCTGGTAACACCTCAAATATGATCAAAACCGGAATCACCACCGCATTTTCTTCTCAATTCCCCGGTTTGAATGGCCTAGGCGTTGCATTAGCTCGCGCAGACTTGGCTGTTGGTGGAGTTGTCCCAATGCACACACACCATGGAGCTTCAGAAATTGTACTTATTATTGAAGGAACCATAACCGCCGGGTTCATTTCCTCAGATAACAAAGTTTATCTAAAAACTCTTAAGAAGGGTGACATCATGGTTTTCCCTCAAGGGCTGTTGCACTTCCAAGTGAATGTTGGTGATACTCCAGCTCTTGAGTTTGTTAGCTTCAGCAGTGATAGCCCCGGTCTGCAGATTCTCCATACCGCACTATTTCAAAACAATTTACCTACTGATCTAATCACGCGATCCACTTTCCTCGACACTGCTGAGATAAAGAGACTCAAGGCTCTTCTTGGTGGTACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

225

Amino Acids

24.06

Weight (kDa)

6.57

Isoelectric Point (pI)

33.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 71 - 214 4.6e-37 Cupin
Cupin_2 PF07883 106 - 173 1e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000361)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20630
fragaria_vesca FvH4_1g08531 FvH4_1g08540 FvH4_1g08541 FvH4_1g18170 FvH4_1g18630 FvH4_2g06460 FvH4_6g36650
malus_domestica MD02G1089800.v1.1 MD15G1216400.v1.1 MD15G1216500.v1.1
prunus_persica Prupe.7G200100_v2.0.a1 Prupe.7G200200_v2.0.a1 Prupe.7G200300_v2.0.a1
pyrus_communis pycom02g07170 pycom15g19190
rosa_chinensis RchiOBHm_Chr2g0094741 RchiOBHm_Chr2g0094751 RchiOBHm_Chr2g0094761 RchiOBHm_Chr2g0094781 RchiOBHm_Chr2g0094791 RchiOBHm_Chr2g0095301 RchiOBHm_Chr2g0108661 RchiOBHm_Chr2g0109441 RchiOBHm_Chr2g0149111
rosa_laevigata RLG00000002180 RLG00000003674 RLG00000016514 RLG00000016515 RLG00000016516 RLG00000016517 RLG00000016521 RLG00000017684 RLG00000017738 RLG00000020370
rosa_multiflora Rmu_co8274667.1_g000001 Rmu_co8326765.1_g000001 Rmu_co8421367.1_g000001 Rmu_sc0000332.1_g000020 Rmu_sc0001588.1_g000008 Rmu_sc0002105.1_g000003 Rmu_sc0002345.1_g000005 Rmu_sc0004359.1_g000003 Rmu_sc0004359.1_g000007 Rmu_sc0004359.1_g000008 Rmu_sc0004359.1_g000010 Rmu_sc0004359.1_g000012 Rmu_sc0004359.1_g000015 Rmu_sc0006422.1_g000015 Rmu_sc0006422.1_g000017 Rmu_sc0006422.1_g000019 Rmu_sc0006422.1_g000021 Rmu_sc0006422.1_g000022 Rmu_sc0012763.1_g000007 Rmu_sc0022670.1_g000002 Rmu_sc0025419.1_g000001
rosa_roxburghii Rroxscaffold_2G00098450 Rroxscaffold_2G00134240 Rroxscaffold_2G00146910 Rroxscaffold_2G00146920 Rroxscaffold_2G00146930 Rroxscaffold_2G00146940 Rroxscaffold_3G00238780
rosa_rugosa Rorug02G0046400.1 Rorug02G0046500.1 Rorug02G0046600.1 Rorug02G0046700.1 Rorug02G0046900 Rorug02G0049100 Rorug02G0155100 Rorug02G0159900 Rorug02G0160200 Rorug02G0409200 Rorug07G0200300
rosa_samantha Rh2AG093700 Rh2AG093800 Rh2AG093900 Rh2AG094000 Rh2AG094100 Rh2AG094400 Rh2AG097200 Rh2AG204600 Rh2AG212100 Rh2AG469000 Rh2BG094200 Rh2BG094400 Rh2BG094700 Rh2BG095000 Rh2BG222500 Rh2BG481500 Rh2DG093300 Rh2DG093400 Rh2DG093500 Rh2DG093700 Rh2DG093800 Rh2DG098300 Rh2DG217700 Rh2DG490400 Rh7AG339600 Rh7CG357600 Rh7DG338300
rosa_wichuraiana Rw2G007340 Rw2G016310 Rw2G038180 Rw7G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 53
Acc36I ACCTGC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 201
AccII CGCG 2 cut(s) 315, 613
AciI CCGC 3 cut(s) 261, 402, 573
AclWI GGATC 1 cut(s) 609
AcuI CTGAAG 2 cut(s) 351, 520
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 2 cut(s) 378, 670
AflII CTTAAG 1 cut(s) 446
AgsI TTSAA 4 cut(s) 8, 289, 389, 584
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 5 cut(s) 137, 311, 365, 518, 534
AluI AGCT 5 cut(s) 137, 311, 365, 518, 534
Alw26I GTCTC 1 cut(s) 640
AlwI GGATC 1 cut(s) 609
AoxI GGCC 2 cut(s) 145, 292
ApeKI GCWGC 1 cut(s) 101
AspA2I CCTAGG 2 cut(s) 211, 295
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 146
AsuC2I CCSGG 3 cut(s) 282, 406, 552
AsuHPI GGTGA 3 cut(s) 247, 467, 518
AvrII CCTAGG 2 cut(s) 211, 295
BbvI GCAGC 1 cut(s) 88
BciT130I CCWGG 1 cut(s) 222
BclI TGATCA 2 cut(s) 36, 240
BcnI CCSGG 3 cut(s) 282, 406, 552
BcoDI GTCTC 1 cut(s) 640
BfaI CTAG 2 cut(s) 212, 296
BfmI CTRYAG 2 cut(s) 150, 557
BfrI CTTAAG 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 1 cut(s) 102
BlnI CCTAGG 2 cut(s) 211, 295
BlsI GCNGC 1 cut(s) 103
Bme1390I CCNGG 4 cut(s) 222, 282, 406, 552
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 394
BmrFI CCNGG 4 cut(s) 222, 282, 406, 552
BpmI CTGGAG 1 cut(s) 498
BpuEI CTTGAG 4 cut(s) 126, 459, 542, 635
BpuMI CCSGG 3 cut(s) 282, 406, 552
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 5 cut(s) 211, 280, 295, 358, 550
BsaWI WCCGGW 1 cut(s) 248
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 5 cut(s) 211, 280, 295, 358, 550
BseGI GGATG 1 cut(s) 196
BseMII CTCAG 2 cut(s) 432, 627
BseRI GAGGAG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 88
Bsh1236I CGCG 2 cut(s) 315, 613
BshFI GGCC 2 cut(s) 147, 294
BsiSI CCGG 4 cut(s) 249, 282, 405, 552
BslFI GGGAC 1 cut(s) 326
BsmAI GTCTC 1 cut(s) 640
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 2 cut(s) 147, 294
Bsp143I GATC 4 cut(s) 36, 240, 601, 614
Bsp19I CCATGG 1 cut(s) 358
BspACI CCGC 3 cut(s) 261, 402, 573
BspANI GGCC 2 cut(s) 147, 294
BspCNI CTCAG 2 cut(s) 431, 628
BspFNI CGCG 2 cut(s) 315, 613
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 394
BspMAI CTGCAG 2 cut(s) 154, 561
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 1 cut(s) 609
BspQI GCTCTTC 1 cut(s) 663
BspTI CTTAAG 1 cut(s) 446
BssECI CCNNGG 5 cut(s) 211, 280, 295, 358, 550
BssMI GATC 4 cut(s) 36, 240, 601, 614
BssNI GRCGYC 1 cut(s) 50
BssT1I CCWWGG 3 cut(s) 211, 295, 358
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 2 cut(s) 94, 663
BstACI GRCGYC 1 cut(s) 50
BstAFI CTTAAG 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 2 cut(s) 418, 636
BstDSI CCRYGG 1 cut(s) 358
BstF5I GGATG 1 cut(s) 196
BstFNI CGCG 2 cut(s) 315, 613
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 4 cut(s) 39, 243, 604, 617
BstMAI GTCTC 1 cut(s) 640
BstMBI GATC 4 cut(s) 36, 240, 601, 614
BstMWI GCNNNNNNNGC 2 cut(s) 323, 546
BstNI CCWGG 1 cut(s) 222
BstSCI CCNGG 4 cut(s) 220, 280, 404, 550
BstSFI CTRYAG 2 cut(s) 150, 557
BstUI CGCG 2 cut(s) 315, 613
BstV1I GCAGC 1 cut(s) 88
BsuRI GGCC 2 cut(s) 147, 294
BtgI CCRYGG 1 cut(s) 358
BtsCI GGATG 1 cut(s) 196
BtsI GCAGTG 2 cut(s) 547, 630
BtsIMutI CAGTG 2 cut(s) 547, 630
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 313
CciI TCATGA 1 cut(s) 39
CfoI GCGC 1 cut(s) 317
Cfr13I GGNCC 1 cut(s) 146
Csp6I GTAC 2 cut(s) 377, 669
CviAII CATG 3 cut(s) 40, 359, 463
CviQI GTAC 2 cut(s) 377, 669
DdeI CTNAG 2 cut(s) 418, 636
DpnI GATC 4 cut(s) 38, 242, 603, 616
DpnII GATC 4 cut(s) 36, 240, 601, 614
Eam1104I CTCTTC 2 cut(s) 94, 663
EarI CTCTTC 2 cut(s) 94, 663
Eco130I CCWWGG 3 cut(s) 211, 295, 358
Eco57I CTGAAG 2 cut(s) 351, 520
EcoO109I RGGNCCY 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 220
EcoT14I CCWWGG 3 cut(s) 211, 295, 358
ErhI CCWWGG 3 cut(s) 211, 295, 358
FaeI CATG 3 cut(s) 43, 362, 466
FaiI YATR 8 cut(s) 18, 41, 99, 239, 360, 398, 464, 570
FaqI GGGAC 1 cut(s) 326
FatI CATG 3 cut(s) 39, 358, 462
FbaI TGATCA 2 cut(s) 36, 240
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 1 cut(s) 102
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 1 cut(s) 102
FspBI CTAG 2 cut(s) 212, 296
GlaI GCGC 1 cut(s) 316
GluI GCNGC 1 cut(s) 102
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 2 cut(s) 147, 294
HapII CCGG 4 cut(s) 249, 282, 405, 552
HhaI GCGC 1 cut(s) 317
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 3 cut(s) 43, 362, 466
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HinfI GANTC 3 cut(s) 252, 562, 648
HpaII CCGG 4 cut(s) 249, 282, 405, 552
HphI GGTGA 3 cut(s) 247, 467, 518
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 2 cut(s) 370, 421
Hpy188III TCNNGA 2 cut(s) 40, 521
Hpy8I GTNNAC 1 cut(s) 202
HpyAV CCTTC 2 cut(s) 383, 445
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 9 cut(s) 109, 125, 152, 165, 176, 305, 349, 487, 559
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 546
HpyF3I CTNAG 2 cut(s) 418, 636
HpySE526I ACGT 1 cut(s) 50
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 3 cut(s) 43, 362, 466
HspAI GCGC 1 cut(s) 315
Ksp22I TGATCA 2 cut(s) 36, 240
Kzo9I GATC 4 cut(s) 36, 240, 601, 614
LguI GCTCTTC 1 cut(s) 663
LmnI GCTCC 2 cut(s) 142, 362
Lsp1109I GCAGC 1 cut(s) 88
MaeI CTAG 2 cut(s) 212, 296
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 4 cut(s) 155, 181, 224, 455
MalI GATC 4 cut(s) 38, 242, 603, 616
MboI GATC 4 cut(s) 36, 240, 601, 614
MboII GAAGA 4 cut(s) 58, 81, 261, 650
MluCI AATT 4 cut(s) 275, 372, 589, 673
MlyI GAGTC 1 cut(s) 642
MnlI CCTC 7 cut(s) 39, 103, 150, 241, 427, 483, 635
MseI TTAA 2 cut(s) 447, 676
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 4 cut(s) 249, 282, 405, 552
MspR9I CCNGG 4 cut(s) 222, 282, 406, 552
MvaI CCWGG 1 cut(s) 222
MvnI CGCG 2 cut(s) 315, 613
MwoI GCNNNNNNNGC 2 cut(s) 323, 546
NciI CCSGG 3 cut(s) 282, 406, 552
NcoI CCATGG 1 cut(s) 358
NdeII GATC 4 cut(s) 36, 240, 601, 614
NlaIII CATG 3 cut(s) 43, 362, 466
NlaIV GGNNCC 1 cut(s) 394
NmuCI GTSAC 1 cut(s) 455
PagI TCATGA 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 663
PfeI GAWTC 2 cut(s) 252, 562
PkrI GCNGC 1 cut(s) 103
PleI GAGTC 1 cut(s) 642
PpsI GAGTC 1 cut(s) 642
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 1 cut(s) 394
PspPI GGNCC 1 cut(s) 146
PstI CTGCAG 2 cut(s) 154, 561
RsaI GTAC 2 cut(s) 378, 670
RsaNI GTAC 2 cut(s) 377, 669
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 2 cut(s) 447, 676
SatI GCNGC 1 cut(s) 102
Sau3AI GATC 4 cut(s) 36, 240, 601, 614
Sau96I GGNCC 1 cut(s) 146
SbfI CCTGCAGG 1 cut(s) 154
SchI GAGTC 1 cut(s) 642
ScrFI CCNGG 4 cut(s) 222, 282, 406, 552
SdaI CCTGCAGG 1 cut(s) 154
SfcI CTRYAG 2 cut(s) 150, 557
SmlI CTYRAG 5 cut(s) 141, 446, 474, 521, 650
SmoI CTYRAG 5 cut(s) 141, 446, 474, 521, 650
Sse8387I CCTGCAGG 1 cut(s) 154
Sse9I AATT 4 cut(s) 275, 372, 589, 673
SsiI CCGC 3 cut(s) 261, 402, 573
SspMI CTAG 2 cut(s) 212, 296
StyD4I CCNGG 4 cut(s) 220, 280, 404, 550
StyI CCWWGG 3 cut(s) 211, 295, 358
TaaI ACNGT 1 cut(s) 187
TaiI ACGT 1 cut(s) 53
TaqI TCGA 1 cut(s) 627
TasI AATT 4 cut(s) 275, 372, 589, 673
TatI WGTACW 1 cut(s) 376
TfiI GAWTC 2 cut(s) 252, 562
Tru1I TTAA 2 cut(s) 447, 676
Tru9I TTAA 2 cut(s) 447, 676
TscAI CASTG 2 cut(s) 547, 637
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 1 cut(s) 101
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 1 cut(s) 400
TspRI CASTG 2 cut(s) 547, 637
Vha464I CTTAAG 1 cut(s) 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmaJI CCTAGG 2 cut(s) 211, 295
XmiI GTMKAC 1 cut(s) 201
XspI CTAG 2 cut(s) 212, 296
ZraI GACGTC 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.