RLG00000016514

Cupin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000361
Physical Location & Seq
Forward (+)
7206994 .. 7212779
5786 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016514

Sequence Viewer

Length: 1170 bp
ATGATTTTCCCTATCTTTTTCACATTTGTTCTCCTCCTTTCCATTTCCGATGCTGCAGTGCAGGACTTCTGTGTTGCAGACTACAAGGCTCCTGAAGGCCCTGCAGGCTACTCCTGCAAGAAGGCTGCAAAAGTTACCGTAGATGACTTTGTATTCTCTGGCCTAGGTGTTGCTGGTAACACCACAAACATTATCAAGGCTGCAGTCACCCCGGCATTTGCTGCTCAATTTCCTGGTGTTAACGGCCTTGGCATTTCGCTGGCTCGTCTAGACCTGGCTCCCGGTGGAGTCATCCCATTTCACACACACCCTGGAGCTTCAGAAGTCTTGATTGTTACGCAAGGAACCATAATTGCCGGGTTCATTTCATCAGCTAACACAGTTTATCTGAAAACCCTTAAGACGGGTGATCTTATGGTGTTCCCTCAAGGGTTATTGCACTTTCAAGTGAATGGAGGTAATACTTCAGCAATTGCCTTCCCTAGCTTCAGTAGTCCAAGCCCCGGTCTGCAGATTCTGGACTTTGCACTTTTCCAAAACGATTTGCCTACATCATTGGTAGCAATGACTACTTTCCTCGATGTTGCTCAGATCAAGAAACTTAAGGGTGTTCTAGGTGACTTGACAGCTCCAGAAGGCCCTGCAGGCTACTCCTGCAAGAAGCCCGCAAAAGTTACCGTAGATGATTTTGTGTTCTCTGGCCTAGGCATTGCTGGTAACACCACCAATATCATCAAAGCTGCAGTCACACCTGCTTTTGCTGCTCAATTTCCTGGTGTCAATGGCCTCGGCATTTCGCTGGCTCGTTTAGACTTGGCTGTTGATGGAGTCATCCCATTTCACACGCACCCTGGAGCTTCAGAAGTCCTGATTGTTGTGAAAGGAACAATCTGTGCCGGGTTTGTTTCCTCAGCTAACAAAGTTTATCTACAAACTCTTGAGAAGGGTGACAGTATGGTTTTCCCTCAAGGTTTGTTGCACTTCCAAGTCAATGGAGGTAATACTCCAGCCCTTGCCTTTGTTAGCTTCAGTAGTCCTAGCCCCGGTCTGCAGATTCTGGACTTTGCATTATTTAAAAATGACTTACCGACCCCATTGATAGCTCAAACCACATTCCTCGACGTTGCCCAGATAAAGAAACTTAAGGGTGTTCTTGGTGGCACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

390

Amino Acids

40.36

Weight (kDa)

6.58

Isoelectric Point (pI)

23.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 54 - 198 2.6e-35 Cupin
Cupin_2 PF07883 90 - 157 2.7e-07 Cupin domain
Cupin_1 PF00190 234 - 378 1.9e-36 Cupin
Cupin_2 PF07883 271 - 341 2e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000361)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20630
fragaria_vesca FvH4_1g08531 FvH4_1g08540 FvH4_1g08541 FvH4_1g18170 FvH4_1g18630 FvH4_2g06460 FvH4_6g36650
malus_domestica MD02G1089800.v1.1 MD15G1216400.v1.1 MD15G1216500.v1.1
prunus_persica Prupe.7G200100_v2.0.a1 Prupe.7G200200_v2.0.a1 Prupe.7G200300_v2.0.a1
pyrus_communis pycom02g07170 pycom15g19190
rosa_chinensis RchiOBHm_Chr2g0094741 RchiOBHm_Chr2g0094751 RchiOBHm_Chr2g0094761 RchiOBHm_Chr2g0094781 RchiOBHm_Chr2g0094791 RchiOBHm_Chr2g0095301 RchiOBHm_Chr2g0108661 RchiOBHm_Chr2g0109441 RchiOBHm_Chr2g0149111
rosa_laevigata RLG00000002180 RLG00000003674 RLG00000016514 RLG00000016515 RLG00000016516 RLG00000016517 RLG00000016521 RLG00000017684 RLG00000017738 RLG00000020370
rosa_multiflora Rmu_co8274667.1_g000001 Rmu_co8326765.1_g000001 Rmu_co8421367.1_g000001 Rmu_sc0000332.1_g000020 Rmu_sc0001588.1_g000008 Rmu_sc0002105.1_g000003 Rmu_sc0002345.1_g000005 Rmu_sc0004359.1_g000003 Rmu_sc0004359.1_g000007 Rmu_sc0004359.1_g000008 Rmu_sc0004359.1_g000010 Rmu_sc0004359.1_g000012 Rmu_sc0004359.1_g000015 Rmu_sc0006422.1_g000015 Rmu_sc0006422.1_g000017 Rmu_sc0006422.1_g000019 Rmu_sc0006422.1_g000021 Rmu_sc0006422.1_g000022 Rmu_sc0012763.1_g000007 Rmu_sc0022670.1_g000002 Rmu_sc0025419.1_g000001
rosa_roxburghii Rroxscaffold_2G00098450 Rroxscaffold_2G00134240 Rroxscaffold_2G00146910 Rroxscaffold_2G00146920 Rroxscaffold_2G00146930 Rroxscaffold_2G00146940 Rroxscaffold_3G00238780
rosa_rugosa Rorug02G0046400.1 Rorug02G0046500.1 Rorug02G0046600.1 Rorug02G0046700.1 Rorug02G0046900 Rorug02G0049100 Rorug02G0155100 Rorug02G0159900 Rorug02G0160200 Rorug02G0409200 Rorug07G0200300
rosa_samantha Rh2AG093700 Rh2AG093800 Rh2AG093900 Rh2AG094000 Rh2AG094100 Rh2AG094400 Rh2AG097200 Rh2AG204600 Rh2AG212100 Rh2AG469000 Rh2BG094200 Rh2BG094400 Rh2BG094700 Rh2BG095000 Rh2BG222500 Rh2BG481500 Rh2DG093300 Rh2DG093400 Rh2DG093500 Rh2DG093700 Rh2DG093800 Rh2DG098300 Rh2DG217700 Rh2DG490400 Rh7AG339600 Rh7CG357600 Rh7DG338300
rosa_wichuraiana Rw2G007340 Rw2G016310 Rw2G038180 Rw7G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 760
Acc36I ACCTGC 1 cut(s) 760
AciI CCGC 1 cut(s) 666
AcuI CTGAAG 6 cut(s) 114, 303, 450, 472, 843, 1012
AfiI CCNNNNNNNGG 3 cut(s) 403, 503, 1043
AflII CTTAAG 3 cut(s) 398, 602, 1142
AgsI TTSAA 1 cut(s) 446
AjnI CCWGG 5 cut(s) 232, 273, 310, 772, 850
AluBI AGCT 9 cut(s) 317, 374, 486, 629, 740, 857, 914, 1026, 1103
AluI AGCT 9 cut(s) 317, 374, 486, 629, 740, 857, 914, 1026, 1103
AlwNI CAGNNNCTG 2 cut(s) 517, 1057
AoxI GGCC 6 cut(s) 97, 160, 244, 637, 700, 784
ApeKI GCWGC 6 cut(s) 53, 125, 200, 221, 740, 761
ArsI GACNNNNNNTTYG 2 cut(s) 729, 761
AspA2I CCTAGG 2 cut(s) 163, 703
AspS9I GGNCC 2 cut(s) 98, 638
AsuC2I CCSGG 6 cut(s) 212, 282, 358, 504, 898, 1044
AsuHPI GGTGA 4 cut(s) 199, 419, 629, 959
AvrII CCTAGG 2 cut(s) 163, 703
BbvCI CCTCAGC 1 cut(s) 910
BbvI GCAGC 6 cut(s) 40, 112, 187, 208, 727, 748
BccI CCATC 1 cut(s) 818
BceAI ACGGC 1 cut(s) 259
BciT130I CCWGG 5 cut(s) 234, 275, 312, 774, 852
BcnI CCSGG 6 cut(s) 212, 282, 358, 504, 898, 1044
BfaI CTAG 6 cut(s) 164, 269, 483, 614, 704, 1038
BfmI CTRYAG 7 cut(s) 54, 102, 201, 509, 642, 741, 1049
BfrI CTTAAG 3 cut(s) 398, 602, 1142
BfuAI ACCTGC 1 cut(s) 760
BglI GCCNNNNNGGC 2 cut(s) 105, 645
BisI GCNGC 6 cut(s) 54, 126, 201, 222, 741, 762
BlnI CCTAGG 2 cut(s) 163, 703
BlsI GCNGC 6 cut(s) 55, 127, 202, 223, 742, 763
BmgT120I GGNCC 2 cut(s) 98, 638
BmiI GGNNCC 3 cut(s) 90, 279, 346
BmsI GCATC 1 cut(s) 40
BpmI CTGGAG 4 cut(s) 333, 615, 873, 990
Bpu10I CCTNAGC 1 cut(s) 910
BpuEI CTTGAG 3 cut(s) 411, 951, 959
BpuMI CCSGG 6 cut(s) 212, 282, 358, 504, 898, 1044
BsaJI CCNNGG 9 cut(s) 163, 210, 247, 310, 502, 703, 787, 850, 1042
Bsc4I CCNNNNNNNGG 3 cut(s) 403, 503, 1043
Bse3DI GCAATG 2 cut(s) 570, 708
BseBI CCWGG 5 cut(s) 234, 275, 312, 774, 852
BseDI CCNNGG 9 cut(s) 163, 210, 247, 310, 502, 703, 787, 850, 1042
BseGI GGATG 2 cut(s) 291, 831
BseLI CCNNNNNNNGG 3 cut(s) 403, 503, 1043
BseMI GCAATG 2 cut(s) 570, 708
BseMII CTCAG 2 cut(s) 602, 924
BseRI GAGGAG 1 cut(s) 23
BseXI GCAGC 6 cut(s) 40, 112, 187, 208, 727, 748
BsgI GTGCAG 1 cut(s) 80
BshFI GGCC 6 cut(s) 99, 162, 246, 639, 702, 786
BsiSI CCGG 6 cut(s) 212, 282, 357, 504, 897, 1044
BslI CCNNNNNNNGG 3 cut(s) 403, 503, 1043
BsnI GGCC 6 cut(s) 99, 162, 246, 639, 702, 786
Bsp143I GATC 2 cut(s) 409, 591
BspACI CCGC 1 cut(s) 666
BspANI GGCC 6 cut(s) 99, 162, 246, 639, 702, 786
BspCNI CTCAG 2 cut(s) 601, 923
BspLI GGNNCC 3 cut(s) 90, 279, 346
BspMAI CTGCAG 7 cut(s) 58, 106, 205, 513, 646, 745, 1053
BspMI ACCTGC 1 cut(s) 760
BspTI CTTAAG 3 cut(s) 398, 602, 1142
BsrDI GCAATG 2 cut(s) 570, 708
BssECI CCNNGG 9 cut(s) 163, 210, 247, 310, 502, 703, 787, 850, 1042
BssMI GATC 2 cut(s) 409, 591
BssT1I CCWWGG 3 cut(s) 163, 247, 703
Bst2UI CCWGG 5 cut(s) 234, 275, 312, 774, 852
Bst4CI ACNGT 4 cut(s) 139, 382, 679, 953
BstAFI CTTAAG 3 cut(s) 398, 602, 1142
BstAPI GCANNNNNTGC 1 cut(s) 221
BstC8I GCNNGC 5 cut(s) 106, 261, 646, 666, 801
BstDEI CTNAG 2 cut(s) 588, 910
BstF5I GGATG 2 cut(s) 291, 831
BstKTI GATC 2 cut(s) 412, 594
BstMBI GATC 2 cut(s) 409, 591
BstMWI GCNNNNNNNGC 6 cut(s) 105, 114, 221, 645, 654, 761
BstNI CCWGG 5 cut(s) 234, 275, 312, 774, 852
BstSFI CTRYAG 7 cut(s) 54, 102, 201, 509, 642, 741, 1049
BstV1I GCAGC 6 cut(s) 40, 112, 187, 208, 727, 748
BstXI CCANNNNNNTGG 1 cut(s) 992
BsuRI GGCC 6 cut(s) 99, 162, 246, 639, 702, 786
BtsCI GGATG 2 cut(s) 291, 831
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 1 cut(s) 63
BveI ACCTGC 1 cut(s) 760
Cac8I GCNNGC 5 cut(s) 106, 261, 646, 666, 801
CaiI CAGNNNCTG 2 cut(s) 517, 1057
Cfr13I GGNCC 2 cut(s) 98, 638
DdeI CTNAG 2 cut(s) 588, 910
DpnI GATC 2 cut(s) 411, 593
DpnII GATC 2 cut(s) 409, 591
DraI TTTAAA 1 cut(s) 1075
Eco130I CCWWGG 3 cut(s) 163, 247, 703
Eco57I CTGAAG 6 cut(s) 114, 303, 450, 472, 843, 1012
EcoO109I RGGNCCY 2 cut(s) 98, 638
EcoRII CCWGG 5 cut(s) 232, 273, 310, 772, 850
EcoT14I CCWWGG 3 cut(s) 163, 247, 703
ErhI CCWWGG 3 cut(s) 163, 247, 703
FaiI YATR 3 cut(s) 350, 416, 956
FauI CCCGC 1 cut(s) 673
Fnu4HI GCNGC 6 cut(s) 54, 126, 201, 222, 741, 762
FokI GGATG 2 cut(s) 278, 818
Fsp4HI GCNGC 6 cut(s) 54, 126, 201, 222, 741, 762
FspBI CTAG 6 cut(s) 164, 269, 483, 614, 704, 1038
GluI GCNGC 6 cut(s) 54, 126, 201, 222, 741, 762
GsuI CTGGAG 4 cut(s) 333, 615, 873, 990
HaeIII GGCC 6 cut(s) 99, 162, 246, 639, 702, 786
HapII CCGG 6 cut(s) 212, 282, 357, 504, 897, 1044
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 4 cut(s) 288, 514, 828, 1054
HpaI GTTAAC 1 cut(s) 241
HpaII CCGG 6 cut(s) 212, 282, 357, 504, 897, 1044
HphI GGTGA 4 cut(s) 199, 419, 629, 959
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 5 cut(s) 49, 322, 390, 591, 862
Hpy188III TCNNGA 9 cut(s) 92, 269, 328, 518, 595, 632, 868, 938, 1058
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 1 cut(s) 1124
HpyAV CCTTC 5 cut(s) 89, 115, 487, 629, 937
HpyCH4III ACNGT 4 cut(s) 139, 382, 679, 953
HpyCH4IV ACGT 1 cut(s) 1122
HpyF10VI GCNNNNNNNGC 6 cut(s) 105, 114, 221, 645, 654, 761
HpyF3I CTNAG 2 cut(s) 588, 910
HpySE526I ACGT 1 cut(s) 1122
KspAI GTTAAC 1 cut(s) 241
Kzo9I GATC 2 cut(s) 409, 591
LmnI GCTCC 5 cut(s) 94, 283, 314, 634, 854
Lsp1109I GCAGC 6 cut(s) 40, 112, 187, 208, 727, 748
LweI GCATC 1 cut(s) 40
MaeI CTAG 6 cut(s) 164, 269, 483, 614, 704, 1038
MaeII ACGT 1 cut(s) 1122
MaeIII GTNAC 9 cut(s) 133, 176, 205, 334, 617, 673, 716, 745, 947
MalI GATC 2 cut(s) 411, 593
MboI GATC 2 cut(s) 409, 591
MfeI CAATTG 1 cut(s) 471
MluCI AATT 5 cut(s) 227, 351, 471, 767, 1165
MlyI GAGTC 2 cut(s) 297, 837
MnlI CCTC 9 cut(s) 44, 435, 449, 587, 797, 919, 975, 989, 1127
MseI TTAA 6 cut(s) 240, 399, 603, 1074, 1143, 1168
MspCI CTTAAG 3 cut(s) 398, 602, 1142
MspI CCGG 6 cut(s) 212, 282, 357, 504, 897, 1044
MunI CAATTG 1 cut(s) 471
MvaI CCWGG 5 cut(s) 234, 275, 312, 774, 852
MwoI GCNNNNNNNGC 6 cut(s) 105, 114, 221, 645, 654, 761
NciI CCSGG 6 cut(s) 212, 282, 358, 504, 898, 1044
NdeII GATC 2 cut(s) 409, 591
NlaIV GGNNCC 3 cut(s) 90, 279, 346
NmeAIII GCCGAG 1 cut(s) 768
NmuCI GTSAC 4 cut(s) 205, 617, 745, 947
PaqCI CACCTGC 1 cut(s) 760
PfeI GAWTC 2 cut(s) 514, 1054
PkrI GCNGC 6 cut(s) 55, 127, 202, 223, 742, 763
PleI GAGTC 2 cut(s) 296, 836
PpsI GAGTC 2 cut(s) 296, 836
Psp6I CCWGG 5 cut(s) 232, 273, 310, 772, 850
PspGI CCWGG 5 cut(s) 232, 273, 310, 772, 850
PspN4I GGNNCC 3 cut(s) 90, 279, 346
PspPI GGNCC 2 cut(s) 98, 638
PstI CTGCAG 7 cut(s) 58, 106, 205, 513, 646, 745, 1053
PstNI CAGNNNCTG 2 cut(s) 517, 1057
SaqAI TTAA 6 cut(s) 240, 399, 603, 1074, 1143, 1168
SatI GCNGC 6 cut(s) 54, 126, 201, 222, 741, 762
Sau3AI GATC 2 cut(s) 409, 591
Sau96I GGNCC 2 cut(s) 98, 638
SbfI CCTGCAGG 2 cut(s) 106, 646
SchI GAGTC 2 cut(s) 297, 837
SdaI CCTGCAGG 2 cut(s) 106, 646
SfaNI GCATC 1 cut(s) 40
SfcI CTRYAG 7 cut(s) 54, 102, 201, 509, 642, 741, 1049
SmlI CTYRAG 6 cut(s) 398, 426, 602, 938, 966, 1142
SmoI CTYRAG 6 cut(s) 398, 426, 602, 938, 966, 1142
Sse8387I CCTGCAGG 2 cut(s) 106, 646
Sse9I AATT 5 cut(s) 227, 351, 471, 767, 1165
SsiI CCGC 1 cut(s) 666
SspMI CTAG 6 cut(s) 164, 269, 483, 614, 704, 1038
StyI CCWWGG 3 cut(s) 163, 247, 703
TaaI ACNGT 4 cut(s) 139, 382, 679, 953
TaiI ACGT 1 cut(s) 1125
TaqI TCGA 2 cut(s) 579, 1119
TasI AATT 5 cut(s) 227, 351, 471, 767, 1165
TfiI GAWTC 2 cut(s) 514, 1054
Tru1I TTAA 6 cut(s) 240, 399, 603, 1074, 1143, 1168
Tru9I TTAA 6 cut(s) 240, 399, 603, 1074, 1143, 1168
TscAI CASTG 1 cut(s) 63
TseFI GTSAC 4 cut(s) 205, 617, 745, 947
TseI GCWGC 6 cut(s) 53, 125, 200, 221, 740, 761
Tsp45I GTSAC 4 cut(s) 205, 617, 745, 947
TspDTI ATGAA 2 cut(s) 352, 357
TspRI CASTG 1 cut(s) 63
Vha464I CTTAAG 3 cut(s) 398, 602, 1142
XbaI TCTAGA 1 cut(s) 268
XmaJI CCTAGG 2 cut(s) 163, 703
XspI CTAG 6 cut(s) 164, 269, 483, 614, 704, 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.