FvH4_1g15580

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001289
Physical Location & Seq
Reverse (-)
8840738 .. 8843031
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15580.t2

Sequence Viewer

Length: 1158 bp
ATGTCGATTGGTTTGGCAAAGAGAAAGATGGTGACGGTTCTGAGCATTGATGGAGGTGGCGTTCGAGGCATAATTCCCAGCACCATCCTCGGCTTTCTTGAATCTAAGCTTCAGGATCTGGATGGAGCAAATGCAAGAATTGCAGACTATTTTGACATAATCGCCGGAACTAGCACCGGTGGATTGGTCACCACCATGCTTACAGCTCCCAACAAGAACAGTAGACCCATGTATGAAGCCAAAGACATCAACAACTTTTATTTTGAACACTGTCCCCAGATTTTCCCTCAGAAAAGACGAAAAAACTTCTTCACATCAATGACAGATGCAATGAAGCAGGGGCCAAAATATGATGGGAAGCACCTGCGCTCAATGACAAACGGGCTACTTGGTGACCTCACTCTCAAGCAGACCTTGACCAATGTGGTCATTCCAACTTTTGATATCAAACACCTTCAGCCTGTGGTTTTCACAACTATAGATGCAAAAGAAAATCCATTGAAGAATGCAAAGCTTTCAGATGTCTGCGTCAGTACCTCTGCAGCTCCATCTTATCTCCCTCCACACTACTTCGAAGTCAAGGATGACCAAGGAAATATCCGCACATATGATCTTGTTGATGGTGGGGTTGCTGCAAATAATCCTACAATGATGGCCATAAGTCACATAAACAGAGAAATGTTGAAGAACAACTCAGAGCCAATGGATGCCACTCGGTTGCAAGTGCTGTCATTGGGCACCGGCTGTGCCAAGTTTGCAGAGAAGTACAGCGCAGTCGCGGCGTCAAAATGGAGTTTGGTAAATTGGATGTTTGATAATGGGAGCACACCTTTGCTGGACATCTTTGGGGATGCAAGTTCTGATATGGTTGACTTTCATGTCTCCACTCTCTTCCAGTCCCTCAATGTCAAGGAGAACTACCTAAGAATTCAGGATGACACATTGAGCGGTGACGCATCATCTGTGGATATAGCAACCGAGACGAATCTGAAAAGCCTTGTGGAGATCGGAAACGGTTTGCTGAAGAAGCCAGTGTCAAGGGTGAATTTGGATACAGGGAGATATGAGAAATGCGAGGGAGAAGCCACCTATGAAGAAGCACTAGTCCGATTTGCCATACAGCTCTCAAAGGGAAGGAAGCTCAGACAAACCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

386

Amino Acids

42.42

Weight (kDa)

7.01

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 20 - 155 3.7e-14 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 372
AasI GACNNNNNNGTC 1 cut(s) 878
Acc36I ACCTGC 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 737
AccBSI CCGCTC 1 cut(s) 948
AccI GTMKAC 1 cut(s) 223
AccII CGCG 1 cut(s) 779
AciI CCGC 3 cut(s) 601, 779, 948
AclWI GGATC 1 cut(s) 123
AcoI YGGCCR 1 cut(s) 654
AcsI RAATTY 2 cut(s) 927, 1045
AcuI CTGAAG 3 cut(s) 95, 440, 1043
AcyI GRCGYC 1 cut(s) 782
AfaI GTAC 2 cut(s) 535, 767
AgeI ACCGGT 1 cut(s) 176
AgsI TTSAA 4 cut(s) 101, 266, 502, 685
AhlI ACTAGT 1 cut(s) 1102
AloI GAACNNNNNNTCC 2 cut(s) 45, 77
AluBI AGCT 6 cut(s) 109, 206, 514, 545, 1123, 1141
AluI AGCT 6 cut(s) 109, 206, 514, 545, 1123, 1141
Alw21I GWGCWC 1 cut(s) 827
Alw26I GTCTC 2 cut(s) 886, 974
AlwI GGATC 1 cut(s) 123
AlwNI CAGNNNCTG 1 cut(s) 118
AoxI GGCC 2 cut(s) 341, 654
ApeKI GCWGC 2 cut(s) 542, 632
ApoI RAATTY 2 cut(s) 927, 1045
AsiGI ACCGGT 1 cut(s) 176
AspLEI GCGC 2 cut(s) 369, 773
AspS9I GGNCC 1 cut(s) 341
AsuHPI GGTGA 5 cut(s) 43, 181, 404, 962, 1054
AsuII TTCGAA 1 cut(s) 573
BaeGI GKGCMC 1 cut(s) 740
BalI TGGCCA 1 cut(s) 656
BanI GGYRCC 1 cut(s) 737
Bbv12I GWGCWC 1 cut(s) 827
BbvI GCAGC 2 cut(s) 554, 619
BccI CCATC 8 cut(s) 22, 44, 92, 116, 347, 556, 614, 646
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciVI GTATCC 1 cut(s) 1045
BcoDI GTCTC 2 cut(s) 886, 974
BcuI ACTAGT 1 cut(s) 1102
BfaI CTAG 2 cut(s) 171, 1103
BfmI CTRYAG 2 cut(s) 477, 540
BfuAI ACCTGC 1 cut(s) 372
BfuI GTATCC 1 cut(s) 1045
BisI GCNGC 3 cut(s) 543, 633, 780
BlsI GCNGC 3 cut(s) 544, 634, 781
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 2 cut(s) 342, 739
BmsI GCATC 5 cut(s) 316, 472, 697, 841, 965
Bpu14I TTCGAA 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 389
BsaHI GRCGYC 1 cut(s) 782
BsaJI CCNNGG 2 cut(s) 88, 589
BsaWI WCCGGW 1 cut(s) 176
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bse118I RCCGGY 2 cut(s) 176, 740
Bse1I ACTGG 2 cut(s) 895, 1031
Bse3DI GCAATG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 88, 589
BseGI GGATG 7 cut(s) 84, 127, 589, 712, 813, 856, 940
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 4 cut(s) 32, 302, 708, 1156
BseNI ACTGG 2 cut(s) 895, 1031
BseSI GKGCMC 1 cut(s) 740
BseXI GCAGC 2 cut(s) 554, 619
BseYI CCCAGC 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 779
BshFI GGCC 2 cut(s) 343, 656
BshNI GGYRCC 1 cut(s) 737
BshTI ACCGGT 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 827
BsiSI CCGG 3 cut(s) 165, 177, 741
BslFI GGGAC 2 cut(s) 258, 883
BsmAI GTCTC 2 cut(s) 886, 974
BsmBI CGTCTC 1 cut(s) 974
BsmFI GGGAC 2 cut(s) 258, 883
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 2 cut(s) 343, 656
Bsp119I TTCGAA 1 cut(s) 573
Bsp1286I GDGCHC 2 cut(s) 740, 827
Bsp143I GATC 3 cut(s) 115, 610, 1005
BspACI CCGC 3 cut(s) 601, 779, 948
BspANI GGCC 2 cut(s) 343, 656
BspCNI CTCAG 4 cut(s) 33, 301, 707, 1155
BspFNI CGCG 1 cut(s) 779
BspLI GGNNCC 2 cut(s) 342, 739
BspMAI CTGCAG 1 cut(s) 544
BspMI ACCTGC 1 cut(s) 372
BspPI GGATC 1 cut(s) 123
BspT104I TTCGAA 1 cut(s) 573
BspT107I GGYRCC 1 cut(s) 737
BsrBI CCGCTC 1 cut(s) 948
BsrDI GCAATG 1 cut(s) 336
BsrFI RCCGGY 2 cut(s) 176, 740
BsrI ACTGG 2 cut(s) 895, 1031
BssAI RCCGGY 2 cut(s) 176, 740
BssECI CCNNGG 2 cut(s) 88, 589
BssMI GATC 3 cut(s) 115, 610, 1005
BssNI GRCGYC 1 cut(s) 782
BssT1I CCWWGG 1 cut(s) 589
Bst4CI ACNGT 4 cut(s) 37, 221, 272, 1016
Bst6I CTCTTC 1 cut(s) 896
BstACI GRCGYC 1 cut(s) 782
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBI TTCGAA 1 cut(s) 573
BstDEI CTNAG 6 cut(s) 41, 105, 288, 694, 923, 1142
BstEII GGTNACC 2 cut(s) 187, 392
BstF5I GGATG 7 cut(s) 84, 127, 589, 712, 813, 856, 940
BstFNI CGCG 1 cut(s) 779
BstHHI GCGC 2 cut(s) 369, 773
BstKTI GATC 3 cut(s) 118, 613, 1008
BstMAI GTCTC 2 cut(s) 886, 974
BstMBI GATC 3 cut(s) 115, 610, 1005
BstMWI GCNNNNNNNGC 5 cut(s) 66, 140, 755, 779, 1027
BstPI GGTNACC 2 cut(s) 187, 392
BstSFI CTRYAG 2 cut(s) 477, 540
BstSLI GKGCMC 1 cut(s) 740
BstUI CGCG 1 cut(s) 779
BstV1I GCAGC 2 cut(s) 554, 619
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BsuI GTATCC 1 cut(s) 1045
BsuRI GGCC 2 cut(s) 343, 656
BtsCI GGATG 7 cut(s) 84, 127, 589, 712, 813, 856, 940
BtsIMutI CAGTG 2 cut(s) 268, 1038
BveI ACCTGC 1 cut(s) 372
CaiI CAGNNNCTG 1 cut(s) 118
CfoI GCGC 2 cut(s) 369, 773
Cfr10I RCCGGY 2 cut(s) 176, 740
Cfr13I GGNCC 1 cut(s) 341
CseI GACGC 3 cut(s) 517, 771, 962
Csp6I GTAC 2 cut(s) 534, 766
CspAI ACCGGT 1 cut(s) 176
CviAII CATG 3 cut(s) 196, 229, 878
CviQI GTAC 2 cut(s) 534, 766
DdeI CTNAG 6 cut(s) 41, 105, 288, 694, 923, 1142
DpnI GATC 3 cut(s) 117, 612, 1007
DpnII GATC 3 cut(s) 115, 610, 1005
DrdI GACNNNNNNGTC 1 cut(s) 878
DseDI GACNNNNNNGTC 1 cut(s) 878
EaeI YGGCCR 1 cut(s) 654
Eam1104I CTCTTC 1 cut(s) 896
EarI CTCTTC 1 cut(s) 896
Eco130I CCWWGG 1 cut(s) 589
Eco32I GATATC 1 cut(s) 445
Eco57I CTGAAG 3 cut(s) 95, 440, 1043
Eco91I GGTNACC 2 cut(s) 187, 392
EcoO65I GGTNACC 2 cut(s) 187, 392
EcoRI GAATTC 1 cut(s) 927
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 589
ErhI CCWWGG 1 cut(s) 589
Esp3I CGTCTC 1 cut(s) 974
FaeI CATG 3 cut(s) 199, 232, 881
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FaqI GGGAC 2 cut(s) 258, 883
FatI CATG 3 cut(s) 195, 228, 877
FauNDI CATATG 1 cut(s) 607
FblI GTMKAC 1 cut(s) 223
Fnu4HI GCNGC 3 cut(s) 543, 633, 780
FokI GGATG 7 cut(s) 71, 134, 596, 719, 820, 863, 947
Fsp4HI GCNGC 3 cut(s) 543, 633, 780
FspBI CTAG 2 cut(s) 171, 1103
GlaI GCGC 2 cut(s) 368, 772
GluI GCNGC 3 cut(s) 543, 633, 780
GsaI CCCAGC 1 cut(s) 81
HaeIII GGCC 2 cut(s) 343, 656
HapII CCGG 3 cut(s) 165, 177, 741
HgaI GACGC 3 cut(s) 517, 771, 962
HhaI GCGC 2 cut(s) 369, 773
Hin1I GRCGYC 1 cut(s) 782
Hin1II CATG 3 cut(s) 199, 232, 881
Hin6I GCGC 2 cut(s) 367, 771
HinP1I GCGC 2 cut(s) 367, 771
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HindIII AAGCTT 2 cut(s) 107, 512
HinfI GANTC 2 cut(s) 101, 985
HpaII CCGG 3 cut(s) 165, 177, 741
HphI GGTGA 5 cut(s) 43, 181, 404, 962, 1054
Hpy166II GTNNAC 2 cut(s) 224, 871
Hpy188I TCNGA 9 cut(s) 42, 291, 520, 697, 862, 990, 1010, 1109, 1145
Hpy188III TCNNGA 4 cut(s) 98, 113, 119, 932
Hpy8I GTNNAC 2 cut(s) 224, 871
HpyAV CCTTC 2 cut(s) 464, 1128
HpyCH4III ACNGT 4 cut(s) 37, 221, 272, 1016
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 140, 755, 779, 1027
HpyF3I CTNAG 6 cut(s) 41, 105, 288, 694, 923, 1142
Hsp92I GRCGYC 1 cut(s) 782
Hsp92II CATG 3 cut(s) 199, 232, 881
HspAI GCGC 2 cut(s) 367, 771
Kzo9I GATC 3 cut(s) 115, 610, 1005
LmnI GCTCC 4 cut(s) 125, 211, 550, 822
Lsp1109I GCAGC 2 cut(s) 554, 619
LweI GCATC 5 cut(s) 316, 472, 697, 841, 965
MaeI CTAG 2 cut(s) 171, 1103
MaeIII GTNAC 5 cut(s) 31, 187, 392, 662, 950
MalI GATC 3 cut(s) 117, 612, 1007
MbiI CCGCTC 1 cut(s) 948
MboI GATC 3 cut(s) 115, 610, 1005
MboII GAAGA 6 cut(s) 301, 514, 697, 883, 1036, 1106
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 2 cut(s) 740, 827
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 5 cut(s) 72, 138, 802, 927, 1045
MluNI TGGCCA 1 cut(s) 656
MmeI TCCRAC 1 cut(s) 458
MnlI CCTC 9 cut(s) 47, 59, 98, 297, 407, 547, 570, 911, 1069
Mox20I TGGCCA 1 cut(s) 656
MscI TGGCCA 1 cut(s) 656
MslI CAYNNNNRTG 2 cut(s) 194, 317
Msp20I TGGCCA 1 cut(s) 656
MspI CCGG 3 cut(s) 165, 177, 741
Mva1269I GAATGC 1 cut(s) 511
MvnI CGCG 1 cut(s) 779
MwoI GCNNNNNNNGC 5 cut(s) 66, 140, 755, 779, 1027
NdeI CATATG 1 cut(s) 607
NdeII GATC 3 cut(s) 115, 610, 1005
NlaIII CATG 3 cut(s) 199, 232, 881
NlaIV GGNNCC 2 cut(s) 342, 739
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 5 cut(s) 31, 187, 392, 662, 950
NspV TTCGAA 1 cut(s) 573
PaqCI CACCTGC 1 cut(s) 372
PctI GAATGC 1 cut(s) 511
PfeI GAWTC 2 cut(s) 101, 985
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 3 cut(s) 544, 634, 781
PspEI GGTNACC 2 cut(s) 187, 392
PspFI CCCAGC 1 cut(s) 77
PspN4I GGNNCC 2 cut(s) 342, 739
PspPI GGNCC 1 cut(s) 341
PstI CTGCAG 1 cut(s) 544
PstNI CAGNNNCTG 1 cut(s) 118
PsuI RGATCY 1 cut(s) 115
RsaI GTAC 2 cut(s) 535, 767
RsaNI GTAC 2 cut(s) 534, 766
RseI CAYNNNNRTG 2 cut(s) 194, 317
SatI GCNGC 3 cut(s) 543, 633, 780
Sau3AI GATC 3 cut(s) 115, 610, 1005
Sau96I GGNCC 1 cut(s) 341
SduI GDGCHC 2 cut(s) 740, 827
SfaNI GCATC 5 cut(s) 316, 472, 697, 841, 965
SfcI CTRYAG 2 cut(s) 477, 540
SfuI TTCGAA 1 cut(s) 573
SgrAI CRCCGGYG 1 cut(s) 176
SmiMI CAYNNNNRTG 2 cut(s) 194, 317
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SpeI ACTAGT 1 cut(s) 1102
Sse9I AATT 5 cut(s) 72, 138, 802, 927, 1045
SsiI CCGC 3 cut(s) 601, 779, 948
SspMI CTAG 2 cut(s) 171, 1103
StyI CCWWGG 1 cut(s) 589
TaaI ACNGT 4 cut(s) 37, 221, 272, 1016
TaqI TCGA 3 cut(s) 5, 64, 573
TasI AATT 5 cut(s) 72, 138, 802, 927, 1045
TatI WGTACW 1 cut(s) 765
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 2 cut(s) 101, 985
TscAI CASTG 2 cut(s) 275, 1038
TseFI GTSAC 5 cut(s) 31, 187, 392, 662, 950
TseI GCWGC 2 cut(s) 542, 632
Tsp45I GTSAC 5 cut(s) 31, 187, 392, 662, 950
TspDTI ATGAA 4 cut(s) 249, 347, 866, 1107
TspRI CASTG 2 cut(s) 275, 1038
XapI RAATTY 2 cut(s) 927, 1045
XmiI GTMKAC 1 cut(s) 223
XspI CTAG 2 cut(s) 171, 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.