FvH4_1g15590

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001289
Physical Location & Seq
Reverse (-)
8843773 .. 8848896
5124 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15590.t3

Sequence Viewer

Length: 1167 bp
ATGGCGACTAGCTTGGAAAAAAGAAAGATGGTGACAGTGCTGAGCATCGATGGAGGTGGCATCAAAGGAATCATTCCCGGCACCATTCTTGCCTTTCTTGAATCCAAGCTTCAGGAATTGGATGGCCGGAATGCAAGACTTGCCGATTATTTTGATATAGTTGCAGGAACAAGCACAGGTGGGTTGGTCACTACCATGCTTACAGCTCCCAACAAGGACAAGCGACCTATGTACGAAGCAAAGGACATCAACAATTTCTACTTTGAACACACACCTAAGATCTTCCCTCAAAGTAGTCAAAAGAATTTCTTGACATCGATTCAAAGTGCGGTTGGTGCTATGATGGGTCCAAAGTACGATGGGAAGTACCTGCGCACAATGACGAGCAAGCTGCTTGGTGACCTTACTCTCAAGGACACTCTAACCAATGTGATCATCCCAGCTTTTGATATCAAACACCTTCAACCTGTGATTTTCTCAACCATTGATGCAAAAGAATGTTCTCTGAAAAATGCAAAGCTTTCGGATATCTGCATCAGTACCTCTGCAGCTCCAAGTTTTCTCCCGGCACACTACTTCGAAGTCCAGGACAACCAGCTGGGAAAAACCCGTACTTTTGATCTCGTTGATGGTGGAGTTGCTGCAAACAATCCTACAATGATGGCTGTAAGCCACATAAACAGAGAGATGTTGAAGCACGGGTCAGAACCCATGGATGCTAATCGGTTGCTAGTGCTATCGTTGGGCACAGGCACTGCCAAATTCGAGAGGAAGTACAGCGCAATTGCGGCCTCAAAATGGGGTTTGATCAATTGGATGTTTCACAATGGCAACACACCTATGATCGACATTTTCTCCGATGCAAGTTCCGATATGGTTGACATTCATGTCTCAACTCTCTTTCAGTCCCTCAATGCCAAAGACAACTACCTACGTATTCAGGATGACAGTTTGAGCGGCGAGGAGGCATCGATGGATATCGCAACAGAGAAGAATCTGAAGAGGCTCGTGGAGATTGGGAAGGGGCTGCTGAAAAAGCCGGTGTCGAGGGTTAATTTGGATACCGGAAAGTACGAGAAATGTGAAGGAGAAGGTACGTACGAAGAAGCACTGGTTGATTTTGCCAAACGGCTCGTGGAGGGGAAGAAGCTCAGGCAACACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

389

Amino Acids

42.83

Weight (kDa)

8.21

Isoelectric Point (pI)

31.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 222 1.5e-26 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 887
Acc16I TGCGCA 1 cut(s) 374
Acc36I ACCTGC 1 cut(s) 378
AccB1I GGYRCC 1 cut(s) 80
AccBSI CCGCTC 1 cut(s) 957
AciI CCGC 3 cut(s) 329, 788, 957
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 2 cut(s) 304, 761
AcuI CTGAAG 2 cut(s) 95, 1019
AfaI GTAC 9 cut(s) 233, 356, 368, 541, 613, 776, 1073, 1096, 1100
AfiI CCNNNNNNNGG 1 cut(s) 798
AgsI TTSAA 5 cut(s) 101, 266, 323, 464, 694
AjnI CCWGG 1 cut(s) 585
AluBI AGCT 9 cut(s) 12, 109, 206, 391, 443, 520, 551, 598, 1150
AluI AGCT 9 cut(s) 12, 109, 206, 391, 443, 520, 551, 598, 1150
Alw26I GTCTC 1 cut(s) 895
AlwNI CAGNNNCTG 1 cut(s) 755
AoxI GGCC 2 cut(s) 124, 789
ApeKI GCWGC 4 cut(s) 391, 548, 641, 1027
ApoI RAATTY 2 cut(s) 304, 761
AspLEI GCGC 2 cut(s) 375, 782
AspS9I GGNCC 1 cut(s) 347
AsuC2I CCSGG 2 cut(s) 78, 566
AsuHPI GGTGA 2 cut(s) 43, 410
AsuII TTCGAA 1 cut(s) 579
AvaII GGWCC 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 749
BanI GGYRCC 1 cut(s) 80
BauI CACGAG 2 cut(s) 1007, 1133
BbvI GCAGC 4 cut(s) 378, 560, 628, 1014
BccI CCATC 8 cut(s) 22, 44, 116, 337, 353, 623, 655, 967
BceAI ACGGC 1 cut(s) 1145
BcgI CGANNNNNNTGC 4 cut(s) 373, 407, 504, 538
BciT130I CCWGG 1 cut(s) 587
BciVI GTATCC 1 cut(s) 1054
BclI TGATCA 2 cut(s) 432, 807
BcnI CCSGG 2 cut(s) 78, 566
BcoDI GTCTC 1 cut(s) 895
BfaI CTAG 2 cut(s) 9, 731
BfmI CTRYAG 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 378
BfuI GTATCC 1 cut(s) 1054
BglII AGATCT 1 cut(s) 279
BisI GCNGC 6 cut(s) 392, 549, 642, 789, 958, 1028
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 6 cut(s) 393, 550, 643, 790, 959, 1029
Bme1390I CCNGG 3 cut(s) 78, 566, 587
Bme18I GGWCC 1 cut(s) 347
BmgT120I GGNCC 1 cut(s) 347
BmiI GGNNCC 2 cut(s) 82, 348
BmrFI CCNGG 3 cut(s) 78, 566, 587
BmsI GCATC 7 cut(s) 54, 69, 478, 543, 706, 850, 977
Bpu10I CCTNAGC 1 cut(s) 1151
Bpu1102I GCTNAGC 1 cut(s) 41
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 2 cut(s) 78, 566
Bsa29I ATCGAT 3 cut(s) 48, 317, 971
BsaAI YACGTR 2 cut(s) 935, 1098
BsaBI GATNNNNATC 2 cut(s) 720, 977
BsaJI CCNNGG 1 cut(s) 711
BsaWI WCCGGW 1 cut(s) 1064
BsaXI ACNNNNNCTCC 2 cut(s) 839, 869
Bsc4I CCNNNNNNNGG 1 cut(s) 798
Bse118I RCCGGY 1 cut(s) 1039
Bse1I ACTGG 1 cut(s) 1116
Bse8I GATNNNNATC 2 cut(s) 720, 977
BseBI CCWGG 1 cut(s) 587
BseCI ATCGAT 3 cut(s) 48, 317, 971
BseDI CCNNGG 1 cut(s) 711
BseGI GGATG 5 cut(s) 127, 435, 721, 822, 949
BseJI GATNNNNATC 2 cut(s) 720, 977
BseLI CCNNNNNNNGG 1 cut(s) 798
BseMII CTCAG 2 cut(s) 32, 1165
BseNI ACTGG 1 cut(s) 1116
BseRI GAGGAG 1 cut(s) 977
BseSI GKGCMC 1 cut(s) 749
BseXI GCAGC 4 cut(s) 378, 560, 628, 1014
BseYI CCCAGC 2 cut(s) 439, 598
BshFI GGCC 2 cut(s) 126, 791
BshNI GGYRCC 1 cut(s) 80
BshVI ATCGAT 3 cut(s) 48, 317, 971
BsiSI CCGG 5 cut(s) 78, 127, 566, 1040, 1065
BsiWI CGTACG 1 cut(s) 1098
BslFI GGGAC 1 cut(s) 892
BslI CCNNNNNNNGG 1 cut(s) 798
BsmAI GTCTC 1 cut(s) 895
BsmFI GGGAC 1 cut(s) 892
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 2 cut(s) 126, 791
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 1 cut(s) 749
Bsp143I GATC 5 cut(s) 279, 432, 619, 807, 843
Bsp1720I GCTNAGC 1 cut(s) 41
Bsp19I CCATGG 1 cut(s) 711
BspACI CCGC 3 cut(s) 329, 788, 957
BspANI GGCC 2 cut(s) 126, 791
BspCNI CTCAG 2 cut(s) 33, 1164
BspDI ATCGAT 3 cut(s) 48, 317, 971
BspLI GGNNCC 2 cut(s) 82, 348
BspMAI CTGCAG 1 cut(s) 550
BspMI ACCTGC 1 cut(s) 378
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 957
BsrFI RCCGGY 1 cut(s) 1039
BsrI ACTGG 1 cut(s) 1116
BssAI RCCGGY 1 cut(s) 1039
BssECI CCNNGG 1 cut(s) 711
BssMI GATC 5 cut(s) 279, 432, 619, 807, 843
BssSI CACGAG 2 cut(s) 1007, 1133
BssT1I CCWWGG 1 cut(s) 711
Bst2BI CACGAG 2 cut(s) 1007, 1133
Bst2UI CCWGG 1 cut(s) 587
Bst4CI ACNGT 2 cut(s) 37, 950
Bst6I CTCTTC 1 cut(s) 995
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBAI YACGTR 2 cut(s) 935, 1098
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 1 cut(s) 389
BstDEI CTNAG 3 cut(s) 41, 276, 1151
BstDSI CCRYGG 1 cut(s) 711
BstEII GGTNACC 1 cut(s) 398
BstF5I GGATG 5 cut(s) 127, 435, 721, 822, 949
BstHHI GCGC 2 cut(s) 375, 782
BstKTI GATC 5 cut(s) 282, 435, 622, 810, 846
BstMAI GTCTC 1 cut(s) 895
BstMBI GATC 5 cut(s) 279, 432, 619, 807, 843
BstMWI GCNNNNNNNGC 4 cut(s) 140, 335, 788, 1036
BstNI CCWGG 1 cut(s) 587
BstPI GGTNACC 1 cut(s) 398
BstSCI CCNGG 3 cut(s) 76, 564, 585
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 749
BstSNI TACGTA 2 cut(s) 935, 1098
BstV1I GCAGC 4 cut(s) 378, 560, 628, 1014
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
Bsu15I ATCGAT 3 cut(s) 48, 317, 971
BsuI GTATCC 1 cut(s) 1054
BsuRI GGCC 2 cut(s) 126, 791
BsuTUI ATCGAT 3 cut(s) 48, 317, 971
BtgI CCRYGG 1 cut(s) 711
BtsCI GGATG 5 cut(s) 127, 435, 721, 822, 949
BtsI GCAGTG 1 cut(s) 753
BtsIMutI CAGTG 3 cut(s) 42, 753, 1109
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 389
CaiI CAGNNNCTG 1 cut(s) 755
CfoI GCGC 2 cut(s) 375, 782
Cfr10I RCCGGY 1 cut(s) 1039
Cfr13I GGNCC 1 cut(s) 347
ClaI ATCGAT 3 cut(s) 48, 317, 971
Csp6I GTAC 9 cut(s) 232, 355, 367, 540, 612, 775, 1072, 1095, 1099
CviAII CATG 3 cut(s) 196, 712, 887
CviQI GTAC 9 cut(s) 232, 355, 367, 540, 612, 775, 1072, 1095, 1099
DdeI CTNAG 3 cut(s) 41, 276, 1151
DpnI GATC 5 cut(s) 281, 434, 621, 809, 845
DpnII GATC 5 cut(s) 279, 432, 619, 807, 843
DrdI GACNNNNNNGTC 1 cut(s) 887
DseDI GACNNNNNNGTC 1 cut(s) 887
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 995
EarI CTCTTC 1 cut(s) 995
Eco105I TACGTA 2 cut(s) 935, 1098
Eco130I CCWWGG 1 cut(s) 711
Eco32I GATATC 3 cut(s) 451, 529, 979
Eco47I GGWCC 1 cut(s) 347
Eco57I CTGAAG 2 cut(s) 95, 1019
Eco91I GGTNACC 1 cut(s) 398
EcoO65I GGTNACC 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 585
EcoRV GATATC 3 cut(s) 451, 529, 979
EcoT14I CCWWGG 1 cut(s) 711
ErhI CCWWGG 1 cut(s) 711
FaeI CATG 3 cut(s) 199, 715, 890
FaiI YATR 9 cut(s) 158, 197, 230, 341, 677, 713, 842, 875, 888
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 1 cut(s) 892
FatI CATG 3 cut(s) 195, 711, 886
FbaI TGATCA 2 cut(s) 432, 807
Fnu4HI GCNGC 6 cut(s) 392, 549, 642, 789, 958, 1028
FokI GGATG 5 cut(s) 134, 422, 728, 829, 956
Fsp4HI GCNGC 6 cut(s) 392, 549, 642, 789, 958, 1028
FspBI CTAG 2 cut(s) 9, 731
FspI TGCGCA 1 cut(s) 374
GlaI GCGC 2 cut(s) 374, 781
GluI GCNGC 6 cut(s) 392, 549, 642, 789, 958, 1028
GsaI CCCAGC 2 cut(s) 443, 602
HaeIII GGCC 2 cut(s) 126, 791
HapII CCGG 5 cut(s) 78, 127, 566, 1040, 1065
HhaI GCGC 2 cut(s) 375, 782
Hin1II CATG 3 cut(s) 199, 715, 890
Hin6I GCGC 2 cut(s) 373, 780
HinP1I GCGC 2 cut(s) 373, 780
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 2 cut(s) 107, 518
HinfI GANTC 4 cut(s) 69, 101, 319, 994
HpaII CCGG 5 cut(s) 78, 127, 566, 1040, 1065
HphI GGTGA 2 cut(s) 43, 410
Hpy166II GTNNAC 1 cut(s) 880
Hpy188I TCNGA 6 cut(s) 507, 526, 706, 859, 871, 999
Hpy188III TCNNGA 5 cut(s) 98, 113, 310, 766, 941
Hpy8I GTNNAC 1 cut(s) 880
HpyAV CCTTC 4 cut(s) 470, 1015, 1079, 1085
HpyCH4III ACNGT 2 cut(s) 37, 950
HpyCH4IV ACGT 2 cut(s) 934, 1097
HpyCH4V TGCA 8 cut(s) 134, 164, 491, 515, 534, 548, 644, 863
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 335, 788, 1036
HpyF3I CTNAG 3 cut(s) 41, 276, 1151
HpySE526I ACGT 2 cut(s) 934, 1097
Hsp92II CATG 3 cut(s) 199, 715, 890
HspAI GCGC 2 cut(s) 373, 780
Ksp22I TGATCA 2 cut(s) 432, 807
Kzo9I GATC 5 cut(s) 279, 432, 619, 807, 843
LmnI GCTCC 2 cut(s) 211, 556
Lsp1109I GCAGC 4 cut(s) 378, 560, 628, 1014
LweI GCATC 7 cut(s) 54, 69, 478, 543, 706, 850, 977
MaeI CTAG 2 cut(s) 9, 731
MaeII ACGT 2 cut(s) 934, 1097
MaeIII GTNAC 3 cut(s) 31, 187, 398
MalI GATC 5 cut(s) 281, 434, 621, 809, 845
MbiI CCGCTC 1 cut(s) 957
MboI GATC 5 cut(s) 279, 432, 619, 807, 843
MboII GAAGA 5 cut(s) 274, 1003, 1012, 1115, 1156
MfeI CAATTG 2 cut(s) 783, 811
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 749
MluCI AATT 7 cut(s) 116, 253, 304, 761, 783, 811, 1054
MseI TTAA 1 cut(s) 1053
MslI CAYNNNNRTG 2 cut(s) 194, 839
MspA1I CMGCKG 1 cut(s) 598
MspI CCGG 5 cut(s) 78, 127, 566, 1040, 1065
MspR9I CCNGG 3 cut(s) 78, 566, 587
MunI CAATTG 2 cut(s) 783, 811
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 1 cut(s) 587
MwoI GCNNNNNNNGC 4 cut(s) 140, 335, 788, 1036
NciI CCSGG 2 cut(s) 78, 566
NcoI CCATGG 1 cut(s) 711
NdeII GATC 5 cut(s) 279, 432, 619, 807, 843
NlaIII CATG 3 cut(s) 199, 715, 890
NlaIV GGNNCC 2 cut(s) 82, 348
NmuCI GTSAC 3 cut(s) 31, 187, 398
NsbI TGCGCA 1 cut(s) 374
NspV TTCGAA 1 cut(s) 579
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 4 cut(s) 69, 101, 319, 994
Pfl23II CGTACG 1 cut(s) 1098
PfoI TCCNGGA 1 cut(s) 585
PkrI GCNGC 6 cut(s) 393, 550, 643, 790, 959, 1029
Ppu21I YACGTR 2 cut(s) 935, 1098
Psp6I CCWGG 1 cut(s) 585
PspEI GGTNACC 1 cut(s) 398
PspFI CCCAGC 2 cut(s) 439, 598
PspGI CCWGG 1 cut(s) 585
PspLI CGTACG 1 cut(s) 1098
PspN4I GGNNCC 2 cut(s) 82, 348
PspPI GGNCC 1 cut(s) 347
PstI CTGCAG 1 cut(s) 550
PstNI CAGNNNCTG 1 cut(s) 755
PsuI RGATCY 1 cut(s) 279
PvuII CAGCTG 1 cut(s) 598
RsaI GTAC 9 cut(s) 233, 356, 368, 541, 613, 776, 1073, 1096, 1100
RsaNI GTAC 9 cut(s) 232, 355, 367, 540, 612, 775, 1072, 1095, 1099
RseI CAYNNNNRTG 2 cut(s) 194, 839
SaqAI TTAA 1 cut(s) 1053
SatI GCNGC 6 cut(s) 392, 549, 642, 789, 958, 1028
Sau3AI GATC 5 cut(s) 279, 432, 619, 807, 843
Sau96I GGNCC 1 cut(s) 347
ScrFI CCNGG 3 cut(s) 78, 566, 587
SduI GDGCHC 1 cut(s) 749
SfaNI GCATC 7 cut(s) 54, 69, 478, 543, 706, 850, 977
SfcI CTRYAG 1 cut(s) 546
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 1 cut(s) 347
SmiMI CAYNNNNRTG 2 cut(s) 194, 839
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
SnaBI TACGTA 2 cut(s) 935, 1098
Sse9I AATT 7 cut(s) 116, 253, 304, 761, 783, 811, 1054
SsiI CCGC 3 cut(s) 329, 788, 957
SspMI CTAG 2 cut(s) 9, 731
StyD4I CCNGG 3 cut(s) 76, 564, 585
StyI CCWWGG 1 cut(s) 711
TaaI ACNGT 2 cut(s) 37, 950
TaiI ACGT 2 cut(s) 937, 1100
TaqI TCGA 7 cut(s) 48, 317, 579, 765, 846, 971, 1046
TasI AATT 7 cut(s) 116, 253, 304, 761, 783, 811, 1054
TatI WGTACW 1 cut(s) 774
TauI GCSGC 2 cut(s) 791, 960
TfiI GAWTC 4 cut(s) 69, 101, 319, 994
Tru1I TTAA 1 cut(s) 1053
Tru9I TTAA 1 cut(s) 1053
TscAI CASTG 3 cut(s) 42, 760, 1116
TseFI GTSAC 3 cut(s) 31, 187, 398
TseI GCWGC 4 cut(s) 391, 548, 641, 1027
Tsp45I GTSAC 3 cut(s) 31, 187, 398
TspDTI ATGAA 1 cut(s) 875
TspRI CASTG 3 cut(s) 42, 760, 1116
VpaK11BI GGWCC 1 cut(s) 347
XapI RAATTY 2 cut(s) 304, 761
XcmI CCANNNNNNNNNTGG 1 cut(s) 1132
XspI CTAG 2 cut(s) 9, 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.