MD15G1280200.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001289
Physical Location & Seq
Reverse (-)
24924455 .. 24926358
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1280200.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGTCGACTGGTTTGACAAAGAGAAGGACGGTGACAGTACTAAGCATTGATGGAGGTGGCATTAGAGGCATAATTCCCAGCACCCTCCTCGCCTTTCTTGAATCCAAGCTTCAGGAATTGGATGGGCCTGATGCAAGAGTCGCAGATTACTTCGACATAATTGCCGGAACAAGCACCGGTGGTCTTGTCACCACCATGCTTGCAGCTCCCAACAAAGACAACAGGCCCATGTTCGAAGCAAAGGATATCAACAACTTCTATTTGGATCACACCCCTAAGATTTTCCCTCAGAATAGTCGTAATAACTTCTTGACATCACTAACAAGTATGGTTGGTGCTGTCATGGGACCAAAATATGATGGAAAGTACCTGCGAACATTGACGAACGAGCTGCTTGGCGATCTCACTCTTAAACAAACCCTAACCACTGTGCTTATTCCAACTTTTGATATTAAGCACCTTCAGCCTGTGATCTTCTCAACCATCGATGCAAAAGAAATTCCACTGAAAAATGCTAGGCTTTCGGATATCTGCGTTAGTACCTCTGCTGCTCCCACTTATCTCCCCGCTCATTACTTCGAGGTCAAAGATGACGAGGGAAGAACCCGAACTTTTGATCTCGTCGATGGTGGGGTAGCTGCAAACAATCCTACGATGACGGCCATAAGCCACATAAACAAAGAGATGTTGAAGCATAACTCAGAGCCCATGGATGCGACAAGGTTGCTAGTGCTATCATTGGGCACAGGCGCAGCCAAGCATGAAGAGAAGTATAATGCAGCCACGGCCTCCAACTGGGGTTTGATCAATTGGTTCTTTGACAATGGGAGCACACCTTTGGTAGACATTTTCGGCGATGCAAGTTCCGATATGGTTGACCTTCATGTCTCCACCCTCTTCCAATCCGTCCACGCCAAGGACAACTACCTCCGTATTCAGGATGACACGTTGATCGGCGAGGAGGCATCATTGGACATTGCAACGGAGAAAAATTTGAAAAGGCTTGTTGAGATCGGGAATGCGTTGTTGAAGAAGCCGGTTTCACGGGTGAATTTGGATACCGGAAAGTATGAGGTATCCAAGGAAGAAGGTACCTATGAAGAAGCACTGATTGATTTTGCCAAAAGGCTCTCGGAGGGAAAGAAGCTTGTAGACAAAACAATTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

390

Amino Acids

42.76

Weight (kDa)

5.47

Isoelectric Point (pI)

29.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 222 2.6e-25 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 884
Acc36I ACCTGC 1 cut(s) 378
Acc65I GGTACC 1 cut(s) 1091
AccB1I GGYRCC 1 cut(s) 1091
AccBSI CCGCTC 1 cut(s) 569
AccI GTMKAC 3 cut(s) 5, 843, 1152
AciI CCGC 1 cut(s) 567
AclWI GGATC 1 cut(s) 273
AcoI YGGCCR 1 cut(s) 660
AcsI RAATTY 3 cut(s) 498, 991, 1051
AcuI CTGAAG 2 cut(s) 95, 446
AfaI GTAC 4 cut(s) 39, 368, 541, 1093
AfiI CCNNNNNNNGG 3 cut(s) 795, 916, 937
AflIII ACRYGT 1 cut(s) 945
AgeI ACCGGT 1 cut(s) 176
AgsI TTSAA 4 cut(s) 101, 691, 997, 1030
AluBI AGCT 5 cut(s) 109, 206, 391, 638, 1147
AluI AGCT 5 cut(s) 109, 206, 391, 638, 1147
Alw21I GWGCWC 1 cut(s) 833
Alw26I GTCTC 1 cut(s) 892
AlwI GGATC 1 cut(s) 273
AoxI GGCC 4 cut(s) 125, 224, 660, 786
ApeKI GCWGC 6 cut(s) 203, 391, 548, 638, 752, 779
ApoI RAATTY 3 cut(s) 498, 991, 1051
AsiGI ACCGGT 1 cut(s) 176
Asp718I GGTACC 1 cut(s) 1091
AspLEI GCGC 1 cut(s) 752
AspS9I GGNCC 3 cut(s) 125, 225, 347
AsuHPI GGTGA 3 cut(s) 43, 181, 1060
AsuII TTCGAA 1 cut(s) 234
AvaII GGWCC 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 746
BanI GGYRCC 1 cut(s) 1091
BanII GRGCYC 1 cut(s) 708
Bbv12I GWGCWC 1 cut(s) 833
BbvI GCAGC 6 cut(s) 215, 378, 535, 625, 764, 791
BccI CCATC 5 cut(s) 44, 116, 353, 491, 620
BceAI ACGGC 2 cut(s) 675, 801
BcgI CGANNNNNNTGC 8 cut(s) 70, 104, 143, 177, 373, 407, 706, 740
BciVI GTATCC 2 cut(s) 1051, 1087
BclI TGATCA 1 cut(s) 804
BcoDI GTCTC 1 cut(s) 892
BfaI CTAG 2 cut(s) 516, 728
BfuAI ACCTGC 1 cut(s) 378
BfuI GTATCC 2 cut(s) 1051, 1087
BisI GCNGC 6 cut(s) 204, 392, 549, 639, 753, 780
BlsI GCNGC 6 cut(s) 205, 393, 550, 640, 754, 781
BmcAI AGTACT 1 cut(s) 39
Bme18I GGWCC 1 cut(s) 347
BmgT120I GGNCC 3 cut(s) 125, 225, 347
BmiI GGNNCC 2 cut(s) 348, 1093
BmrI ACTGGG 1 cut(s) 805
BmsI GCATC 5 cut(s) 121, 478, 703, 847, 974
BmuI ACTGGG 1 cut(s) 805
Bpu14I TTCGAA 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 486
BsaJI CCNNGG 4 cut(s) 708, 783, 915, 1080
BsaWI WCCGGW 2 cut(s) 176, 1061
Bsc4I CCNNNNNNNGG 3 cut(s) 795, 916, 937
Bse118I RCCGGY 2 cut(s) 176, 1036
Bse1I ACTGG 2 cut(s) 13, 800
Bse3DI GCAATG 1 cut(s) 975
BseCI ATCGAT 1 cut(s) 486
BseDI CCNNGG 4 cut(s) 708, 783, 915, 1080
BseGI GGATG 3 cut(s) 127, 718, 946
BseLI CCNNNNNNNGG 3 cut(s) 795, 916, 937
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 2 cut(s) 302, 714
BseNI ACTGG 2 cut(s) 13, 800
BseRI GAGGAG 2 cut(s) 77, 974
BseSI GKGCMC 1 cut(s) 746
BseXI GCAGC 6 cut(s) 215, 378, 535, 625, 764, 791
BseYI CCCAGC 1 cut(s) 77
BshFI GGCC 4 cut(s) 127, 226, 662, 788
BshNI GGYRCC 1 cut(s) 1091
BshTI ACCGGT 1 cut(s) 176
BshVI ATCGAT 1 cut(s) 486
BsiHKAI GWGCWC 1 cut(s) 833
BsiSI CCGG 4 cut(s) 165, 177, 1037, 1062
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 3 cut(s) 795, 916, 937
BsmAI GTCTC 1 cut(s) 892
BsmFI GGGAC 1 cut(s) 360
BsmI GAATGC 1 cut(s) 1024
BsnI GGCC 4 cut(s) 127, 226, 662, 788
Bsp119I TTCGAA 1 cut(s) 234
Bsp1286I GDGCHC 3 cut(s) 708, 746, 833
Bsp143I GATC 7 cut(s) 265, 400, 471, 616, 804, 951, 1011
Bsp19I CCATGG 1 cut(s) 708
BspACI CCGC 1 cut(s) 567
BspANI GGCC 4 cut(s) 127, 226, 662, 788
BspCNI CTCAG 2 cut(s) 301, 713
BspDI ATCGAT 1 cut(s) 486
BspLI GGNNCC 2 cut(s) 348, 1093
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 1 cut(s) 273
BspT104I TTCGAA 1 cut(s) 234
BspT107I GGYRCC 1 cut(s) 1091
BsrBI CCGCTC 1 cut(s) 569
BsrDI GCAATG 1 cut(s) 975
BsrFI RCCGGY 2 cut(s) 176, 1036
BsrI ACTGG 2 cut(s) 13, 800
BssAI RCCGGY 2 cut(s) 176, 1036
BssECI CCNNGG 4 cut(s) 708, 783, 915, 1080
BssMI GATC 7 cut(s) 265, 400, 471, 616, 804, 951, 1011
BssT1I CCWWGG 3 cut(s) 708, 915, 1080
Bst4CI ACNGT 3 cut(s) 31, 37, 430
Bst6I CTCTTC 2 cut(s) 759, 902
BstBI TTCGAA 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 4 cut(s) 41, 276, 288, 700
BstDSI CCRYGG 2 cut(s) 708, 783
BstF5I GGATG 3 cut(s) 127, 718, 946
BstHHI GCGC 1 cut(s) 752
BstKTI GATC 7 cut(s) 268, 403, 474, 619, 807, 954, 1014
BstMAI GTCTC 1 cut(s) 892
BstMBI GATC 7 cut(s) 265, 400, 471, 616, 804, 951, 1011
BstMWI GCNNNNNNNGC 4 cut(s) 66, 140, 463, 785
BstSLI GKGCMC 1 cut(s) 746
BstV1I GCAGC 6 cut(s) 215, 378, 535, 625, 764, 791
Bsu15I ATCGAT 1 cut(s) 486
BsuI GTATCC 2 cut(s) 1051, 1087
BsuRI GGCC 4 cut(s) 127, 226, 662, 788
BsuTUI ATCGAT 1 cut(s) 486
BtgI CCRYGG 2 cut(s) 708, 783
BtgZI GCGATG 1 cut(s) 870
BtsCI GGATG 3 cut(s) 127, 718, 946
BtsIMutI CAGTG 3 cut(s) 426, 503, 1106
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 201
CfoI GCGC 1 cut(s) 752
Cfr10I RCCGGY 2 cut(s) 176, 1036
Cfr13I GGNCC 3 cut(s) 125, 225, 347
ClaI ATCGAT 1 cut(s) 486
Csp6I GTAC 4 cut(s) 38, 367, 540, 1092
CspAI ACCGGT 1 cut(s) 176
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 6 cut(s) 196, 229, 343, 709, 761, 884
CviQI GTAC 4 cut(s) 38, 367, 540, 1092
DdeI CTNAG 4 cut(s) 41, 276, 288, 700
DpnI GATC 7 cut(s) 267, 402, 473, 618, 806, 953, 1013
DpnII GATC 7 cut(s) 265, 400, 471, 616, 804, 951, 1011
DrdI GACNNNNNNGTC 1 cut(s) 884
DseDI GACNNNNNNGTC 1 cut(s) 884
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 2 cut(s) 759, 902
EarI CTCTTC 2 cut(s) 759, 902
Eco130I CCWWGG 3 cut(s) 708, 915, 1080
Eco24I GRGCYC 1 cut(s) 708
Eco32I GATATC 2 cut(s) 247, 529
Eco47I GGWCC 1 cut(s) 347
Eco57I CTGAAG 2 cut(s) 95, 446
EcoRV GATATC 2 cut(s) 247, 529
EcoT14I CCWWGG 3 cut(s) 708, 915, 1080
EcoT38I GRGCYC 1 cut(s) 708
ErhI CCWWGG 3 cut(s) 708, 915, 1080
FaeI CATG 6 cut(s) 199, 232, 346, 712, 764, 887
FaqI GGGAC 1 cut(s) 360
FatI CATG 6 cut(s) 195, 228, 342, 708, 760, 883
FauI CCCGC 1 cut(s) 574
FbaI TGATCA 1 cut(s) 804
FblI GTMKAC 3 cut(s) 5, 843, 1152
Fnu4HI GCNGC 6 cut(s) 204, 392, 549, 639, 753, 780
FokI GGATG 3 cut(s) 134, 725, 953
FriOI GRGCYC 1 cut(s) 708
Fsp4HI GCNGC 6 cut(s) 204, 392, 549, 639, 753, 780
FspBI CTAG 2 cut(s) 516, 728
GlaI GCGC 1 cut(s) 751
GluI GCNGC 6 cut(s) 204, 392, 549, 639, 753, 780
GsaI CCCAGC 1 cut(s) 81
HaeIII GGCC 4 cut(s) 127, 226, 662, 788
HapII CCGG 4 cut(s) 165, 177, 1037, 1062
HhaI GCGC 1 cut(s) 752
Hin1II CATG 6 cut(s) 199, 232, 346, 712, 764, 887
Hin6I GCGC 1 cut(s) 750
HinP1I GCGC 1 cut(s) 750
HincII GTYRAC 2 cut(s) 6, 877
HindII GTYRAC 2 cut(s) 6, 877
HindIII AAGCTT 2 cut(s) 107, 1145
HinfI GANTC 2 cut(s) 101, 138
HpaII CCGG 4 cut(s) 165, 177, 1037, 1062
HphI GGTGA 3 cut(s) 43, 181, 1060
Hpy166II GTNNAC 5 cut(s) 6, 844, 877, 910, 1153
Hpy188I TCNGA 5 cut(s) 291, 526, 703, 868, 1135
Hpy188III TCNNGA 5 cut(s) 98, 113, 310, 938, 1015
Hpy8I GTNNAC 5 cut(s) 6, 844, 877, 910, 1153
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 4 cut(s) 18, 470, 890, 1082
HpyCH4III ACNGT 3 cut(s) 31, 37, 430
HpyCH4IV ACGT 1 cut(s) 947
HpyCH4V TGCA 7 cut(s) 134, 203, 491, 641, 779, 860, 980
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 140, 463, 785
HpyF3I CTNAG 4 cut(s) 41, 276, 288, 700
HpySE526I ACGT 1 cut(s) 947
Hsp92II CATG 6 cut(s) 199, 232, 346, 712, 764, 887
HspAI GCGC 1 cut(s) 750
KpnI GGTACC 1 cut(s) 1095
Ksp22I TGATCA 1 cut(s) 804
Kzo9I GATC 7 cut(s) 265, 400, 471, 616, 804, 951, 1011
LmnI GCTCC 3 cut(s) 211, 556, 828
Lsp1109I GCAGC 6 cut(s) 215, 378, 535, 625, 764, 791
LweI GCATC 5 cut(s) 121, 478, 703, 847, 974
MaeI CTAG 2 cut(s) 516, 728
MaeII ACGT 1 cut(s) 947
MaeIII GTNAC 2 cut(s) 31, 187
MalI GATC 7 cut(s) 267, 402, 473, 618, 806, 953, 1013
MbiI CCGCTC 1 cut(s) 569
MboI GATC 7 cut(s) 265, 400, 471, 616, 804, 951, 1011
MboII GAAGA 7 cut(s) 466, 612, 776, 889, 1042, 1097, 1112
MfeI CAATTG 1 cut(s) 808
MhlI GDGCHC 3 cut(s) 708, 746, 833
MluCI AATT 8 cut(s) 72, 116, 159, 498, 808, 991, 1051, 1161
MlyI GAGTC 1 cut(s) 147
MmeI TCCRAC 2 cut(s) 464, 816
MseI TTAA 3 cut(s) 411, 453, 1164
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 4 cut(s) 165, 177, 1037, 1062
MunI CAATTG 1 cut(s) 808
Mva1269I GAATGC 1 cut(s) 1024
MwoI GCNNNNNNNGC 4 cut(s) 66, 140, 463, 785
NcoI CCATGG 1 cut(s) 708
NdeII GATC 7 cut(s) 265, 400, 471, 616, 804, 951, 1011
NlaIII CATG 6 cut(s) 199, 232, 346, 712, 764, 887
NlaIV GGNNCC 2 cut(s) 348, 1093
NmuCI GTSAC 2 cut(s) 31, 187
NspV TTCGAA 1 cut(s) 234
PctI GAATGC 1 cut(s) 1024
PfeI GAWTC 1 cut(s) 101
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 6 cut(s) 205, 393, 550, 640, 754, 781
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PspFI CCCAGC 1 cut(s) 77
PspN4I GGNNCC 2 cut(s) 348, 1093
PspPI GGNCC 3 cut(s) 125, 225, 347
RsaI GTAC 4 cut(s) 39, 368, 541, 1093
RsaNI GTAC 4 cut(s) 38, 367, 540, 1092
RseI CAYNNNNRTG 1 cut(s) 194
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 411, 453, 1164
SatI GCNGC 6 cut(s) 204, 392, 549, 639, 753, 780
Sau3AI GATC 7 cut(s) 265, 400, 471, 616, 804, 951, 1011
Sau96I GGNCC 3 cut(s) 125, 225, 347
ScaI AGTACT 1 cut(s) 39
SchI GAGTC 1 cut(s) 147
SduI GDGCHC 3 cut(s) 708, 746, 833
SfaNI GCATC 5 cut(s) 121, 478, 703, 847, 974
SfuI TTCGAA 1 cut(s) 234
SgrAI CRCCGGYG 1 cut(s) 176
SinI GGWCC 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 8 cut(s) 72, 116, 159, 498, 808, 991, 1051, 1161
SsiI CCGC 1 cut(s) 567
SspMI CTAG 2 cut(s) 516, 728
StyI CCWWGG 3 cut(s) 708, 915, 1080
TaaI ACNGT 3 cut(s) 31, 37, 430
TaiI ACGT 1 cut(s) 950
TaqI TCGA 6 cut(s) 5, 153, 234, 486, 579, 624
TasI AATT 8 cut(s) 72, 116, 159, 498, 808, 991, 1051, 1161
TatI WGTACW 1 cut(s) 37
TfiI GAWTC 1 cut(s) 101
Tru1I TTAA 3 cut(s) 411, 453, 1164
Tru9I TTAA 3 cut(s) 411, 453, 1164
TscAI CASTG 3 cut(s) 433, 510, 1113
TseFI GTSAC 2 cut(s) 31, 187
TseI GCWGC 6 cut(s) 203, 391, 548, 638, 752, 779
Tsp45I GTSAC 2 cut(s) 31, 187
TspDTI ATGAA 3 cut(s) 777, 872, 1113
TspGWI ACGGA 3 cut(s) 895, 920, 998
TspRI CASTG 3 cut(s) 433, 510, 1113
VpaK11BI GGWCC 1 cut(s) 347
XapI RAATTY 3 cut(s) 498, 991, 1051
XmiI GTMKAC 3 cut(s) 5, 843, 1152
XspI CTAG 2 cut(s) 516, 728
ZrmI AGTACT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.