RLG00000017367

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001289
Physical Location & Seq
Reverse (-)
15776923 .. 15778935
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017367

Sequence Viewer

Length: 1167 bp
ATGTCCATTGGTTTGGCAAAGAGAAAGATGGTGACAGTTCTGAGCATTGATGGAGGTGGCATTAGAGGCATAATTCCCGGCACCATCCTCGGCTTTCTCGAATCTAAGCTTCAGGATTGGGATGGACCAAATGCAAGAATTGCAGATTATTTTGACATAATCGCCGGAACTAGCACCGGGGGATTGGTCACCACCATGCTTACAGCTCCCAGTAAGAACAACCGGCCCATGTATGAAGCAAAGGACATCAACAACTTTTATTTAGAACACTGTCCCCAGATTTTCCCTCAAAAAAGTCGGAAAAAGAAAAACTTCTTCACATCGATAACAAATGCAATGAAGCGGGGGCCAAAATACGATGGGATGTATCTGCGCTCATTGATAAACGGGCTCCTTGGTGATCTCACTCTCAAGCAGACTTTAACCAATGTGGTCATTCCAAGTTTTGATATCAAACACCTTCAGCCTGTGGTTTTCTCAACCATCGATGTTAAATTTATGGTAGTACGGGCAAAAGAAAATTCATTGAAGAATGCAAAGCTTTCAGATGTCTGCATCAGTACCTCTGCAGCTCCATATTATTTCCCCCCACACTACTTCGAAGTCAAGGATGACCAGGGAAATATCCGCACGTTTGATCTTGTCGATGGTGGGGTTGCTGCAAATAATCCTCCATATAAACAGAGAGAGTTGAAGAACAACTCAGAACCAATAAATGCGAATCGCTTGCAAGTGCTGTCATTGGGCACTGGCGCTGCCAAGTTGGAGGAGAAGTACAGTGCAGTCGAGGCATCAAAATGGGGTTTGATGAATTGGATGTTTAATAATGGGAGCACACCTTTGCTGGACATTTTTGGGGATGCAAGTTCTGATATGGTTGACTTTCATGTCTCCACTCTCTTCCAGTCCCTCCATGTCAAGGACAATTACCTACGAATTCAGGATGACACACTAAGCGGAGATGAATCATCAGTGGATATCGCAACCGAGAAGAATCTGAAATGCCTTGTGGAGATCGGAAATGTGTTGCTGAAGAAGCCAGTGTCAAGGGTAAATCTCGATACTGGGAGGTACGAGGAATCTGAGGGAGAAGGTACCTACGAAGAAGCACTGGTCGATTTTGCCAAACGGCTCGCGGAGGGAAAGAAGCTCTGGCAAAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

389

Amino Acids

43.18

Weight (kDa)

7.63

Isoelectric Point (pI)

34.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 224 6e-28 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 887
Acc65I GGTACC 1 cut(s) 1094
AccB1I GGYRCC 2 cut(s) 80, 1094
AccII CGCG 1 cut(s) 1136
AciI CCGC 4 cut(s) 343, 628, 957, 1136
AcsI RAATTY 3 cut(s) 494, 520, 936
AcuI CTGAAG 3 cut(s) 95, 446, 1052
AfaI GTAC 5 cut(s) 507, 562, 776, 1073, 1096
AfiI CCNNNNNNNGG 1 cut(s) 919
AgsI TTSAA 2 cut(s) 529, 694
AjnI CCWGG 1 cut(s) 615
AluBI AGCT 5 cut(s) 109, 206, 541, 572, 1150
AluI AGCT 5 cut(s) 109, 206, 541, 572, 1150
Alw21I GWGCWC 1 cut(s) 836
Alw26I GTCTC 1 cut(s) 895
AoxI GGCC 2 cut(s) 224, 347
ApeKI GCWGC 3 cut(s) 569, 659, 755
ApoI RAATTY 3 cut(s) 494, 520, 936
Asp700I GAANNNNTTC 1 cut(s) 311
Asp718I GGTACC 1 cut(s) 1094
AspLEI GCGC 2 cut(s) 375, 755
AspS9I GGNCC 3 cut(s) 125, 225, 347
AsuC2I CCSGG 2 cut(s) 78, 178
AsuHPI GGTGA 3 cut(s) 43, 181, 410
AsuII TTCGAA 1 cut(s) 600
AvaII GGWCC 1 cut(s) 125
BaeGI GKGCMC 1 cut(s) 749
BanI GGYRCC 2 cut(s) 80, 1094
BanII GRGCYC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 836
BbvI GCAGC 3 cut(s) 581, 646, 742
BccI CCATC 7 cut(s) 22, 44, 92, 116, 353, 491, 641
BceAI ACGGC 1 cut(s) 1145
BcgI CGANNNNNNTGC 4 cut(s) 70, 104, 709, 743
BciT130I CCWGG 1 cut(s) 617
BcnI CCSGG 2 cut(s) 78, 178
BcoDI GTCTC 1 cut(s) 895
BfaI CTAG 1 cut(s) 171
BfmI CTRYAG 1 cut(s) 567
BfoI RGCGCY 1 cut(s) 756
BisI GCNGC 3 cut(s) 570, 660, 756
BlsI GCNGC 3 cut(s) 571, 661, 757
Bme1390I CCNGG 3 cut(s) 78, 178, 617
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 3 cut(s) 125, 225, 347
BmiI GGNNCC 4 cut(s) 82, 348, 392, 1096
BmrFI CCNGG 3 cut(s) 78, 178, 617
BmrI ACTGGG 2 cut(s) 204, 1074
BmsI GCATC 3 cut(s) 564, 800, 850
BmuI ACTGGG 2 cut(s) 204, 1074
Bpu14I TTCGAA 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 2 cut(s) 78, 178
Bsa29I ATCGAT 2 cut(s) 323, 486
BsaJI CCNNGG 4 cut(s) 88, 177, 394, 616
BsaXI ACNNNNNCTCC 2 cut(s) 758, 788
Bsc4I CCNNNNNNNGG 1 cut(s) 919
Bse118I RCCGGY 1 cut(s) 222
Bse1I ACTGG 6 cut(s) 210, 754, 904, 1040, 1069, 1116
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 617
BseCI ATCGAT 2 cut(s) 323, 486
BseDI CCNNGG 4 cut(s) 88, 177, 394, 616
BseGI GGATG 7 cut(s) 84, 127, 369, 616, 822, 865, 949
BseLI CCNNNNNNNGG 1 cut(s) 919
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 3 cut(s) 32, 717, 1074
BseNI ACTGG 6 cut(s) 210, 754, 904, 1040, 1069, 1116
BseRI GAGGAG 1 cut(s) 782
BseSI GKGCMC 1 cut(s) 749
BseXI GCAGC 3 cut(s) 581, 646, 742
BsgI GTGCAG 1 cut(s) 801
Bsh1236I CGCG 1 cut(s) 1136
BshFI GGCC 2 cut(s) 226, 349
BshNI GGYRCC 2 cut(s) 80, 1094
BshVI ATCGAT 2 cut(s) 323, 486
BsiHKAI GWGCWC 1 cut(s) 836
BsiSI CCGG 4 cut(s) 78, 165, 177, 223
BslFI GGGAC 2 cut(s) 258, 892
BslI CCNNNNNNNGG 1 cut(s) 919
BsmAI GTCTC 1 cut(s) 895
BsmFI GGGAC 2 cut(s) 258, 892
BsmI GAATGC 1 cut(s) 538
BsnI GGCC 2 cut(s) 226, 349
Bsp119I TTCGAA 1 cut(s) 600
Bsp1286I GDGCHC 3 cut(s) 393, 749, 836
Bsp143I GATC 3 cut(s) 400, 637, 1014
BspACI CCGC 4 cut(s) 343, 628, 957, 1136
BspANI GGCC 2 cut(s) 226, 349
BspCNI CTCAG 3 cut(s) 33, 716, 1075
BspDI ATCGAT 2 cut(s) 323, 486
BspFNI CGCG 1 cut(s) 1136
BspLI GGNNCC 4 cut(s) 82, 348, 392, 1096
BspMAI CTGCAG 1 cut(s) 571
BspT104I TTCGAA 1 cut(s) 600
BspT107I GGYRCC 2 cut(s) 80, 1094
BsrDI GCAATG 1 cut(s) 342
BsrFI RCCGGY 1 cut(s) 222
BsrI ACTGG 6 cut(s) 210, 754, 904, 1040, 1069, 1116
BssAI RCCGGY 1 cut(s) 222
BssECI CCNNGG 4 cut(s) 88, 177, 394, 616
BssMI GATC 3 cut(s) 400, 637, 1014
BssT1I CCWWGG 1 cut(s) 394
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 3 cut(s) 37, 272, 779
Bst6I CTCTTC 1 cut(s) 905
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBI TTCGAA 1 cut(s) 600
BstC8I GCNNGC 2 cut(s) 728, 1134
BstDEI CTNAG 5 cut(s) 41, 105, 703, 953, 1083
BstEII GGTNACC 1 cut(s) 187
BstF5I GGATG 7 cut(s) 84, 127, 369, 616, 822, 865, 949
BstFNI CGCG 1 cut(s) 1136
BstH2I RGCGCY 1 cut(s) 756
BstHHI GCGC 2 cut(s) 375, 755
BstKTI GATC 3 cut(s) 403, 640, 1017
BstMAI GTCTC 1 cut(s) 895
BstMBI GATC 3 cut(s) 400, 637, 1014
BstMWI GCNNNNNNNGC 4 cut(s) 66, 140, 788, 1036
BstNI CCWGG 1 cut(s) 617
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 3 cut(s) 76, 176, 615
BstSFI CTRYAG 1 cut(s) 567
BstSLI GKGCMC 1 cut(s) 749
BstUI CGCG 1 cut(s) 1136
BstV1I GCAGC 3 cut(s) 581, 646, 742
BstXI CCANNNNNNTGG 1 cut(s) 13
Bsu15I ATCGAT 2 cut(s) 323, 486
BsuRI GGCC 2 cut(s) 226, 349
BsuTUI ATCGAT 2 cut(s) 323, 486
BtsCI GGATG 7 cut(s) 84, 127, 369, 616, 822, 865, 949
BtsIMutI CAGTG 6 cut(s) 268, 747, 784, 978, 1047, 1109
Cac8I GCNNGC 2 cut(s) 728, 1134
CfoI GCGC 2 cut(s) 375, 755
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 3 cut(s) 125, 225, 347
ClaI ATCGAT 2 cut(s) 323, 486
Csp6I GTAC 5 cut(s) 506, 561, 775, 1072, 1095
CviAII CATG 4 cut(s) 196, 229, 887, 914
CviQI GTAC 5 cut(s) 506, 561, 775, 1072, 1095
DdeI CTNAG 5 cut(s) 41, 105, 703, 953, 1083
DpnI GATC 3 cut(s) 402, 639, 1016
DpnII GATC 3 cut(s) 400, 637, 1014
DrdI GACNNNNNNGTC 1 cut(s) 887
DseDI GACNNNNNNGTC 1 cut(s) 887
Eam1104I CTCTTC 1 cut(s) 905
EarI CTCTTC 1 cut(s) 905
Eco130I CCWWGG 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 393
Eco32I GATATC 2 cut(s) 451, 979
Eco47I GGWCC 1 cut(s) 125
Eco57I CTGAAG 3 cut(s) 95, 446, 1052
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
EcoRI GAATTC 1 cut(s) 936
EcoRII CCWGG 1 cut(s) 615
EcoRV GATATC 2 cut(s) 451, 979
EcoT14I CCWWGG 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 394
FaeI CATG 4 cut(s) 199, 232, 890, 917
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 2 cut(s) 258, 892
FatI CATG 4 cut(s) 195, 228, 886, 913
FauI CCCGC 1 cut(s) 336
Fnu4HI GCNGC 3 cut(s) 570, 660, 756
FokI GGATG 7 cut(s) 71, 134, 376, 623, 829, 872, 956
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 3 cut(s) 570, 660, 756
FspBI CTAG 1 cut(s) 171
GlaI GCGC 2 cut(s) 374, 754
GluI GCNGC 3 cut(s) 570, 660, 756
HaeII RGCGCY 1 cut(s) 756
HaeIII GGCC 2 cut(s) 226, 349
HapII CCGG 4 cut(s) 78, 165, 177, 223
HhaI GCGC 2 cut(s) 375, 755
Hin1II CATG 4 cut(s) 199, 232, 890, 917
Hin6I GCGC 2 cut(s) 373, 753
HinP1I GCGC 2 cut(s) 373, 753
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 2 cut(s) 107, 539
HinfI GANTC 5 cut(s) 101, 721, 965, 994, 1079
HpaII CCGG 4 cut(s) 78, 165, 177, 223
HphI GGTGA 3 cut(s) 43, 181, 410
Hpy166II GTNNAC 1 cut(s) 880
Hpy188I TCNGA 8 cut(s) 42, 300, 547, 706, 871, 999, 1019, 1084
Hpy188III TCNNGA 4 cut(s) 98, 113, 941, 1058
Hpy8I GTNNAC 1 cut(s) 880
HpyAV CCTTC 2 cut(s) 470, 1085
HpyCH4III ACNGT 3 cut(s) 37, 272, 779
HpyCH4IV ACGT 1 cut(s) 632
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 140, 788, 1036
HpyF3I CTNAG 5 cut(s) 41, 105, 703, 953, 1083
HpySE526I ACGT 1 cut(s) 632
Hsp92II CATG 4 cut(s) 199, 232, 890, 917
HspAI GCGC 2 cut(s) 373, 753
KpnI GGTACC 1 cut(s) 1098
Kzo9I GATC 3 cut(s) 400, 637, 1014
LmnI GCTCC 4 cut(s) 211, 396, 577, 831
Lsp1109I GCAGC 3 cut(s) 581, 646, 742
LweI GCATC 3 cut(s) 564, 800, 850
MaeI CTAG 1 cut(s) 171
MaeII ACGT 1 cut(s) 632
MaeIII GTNAC 2 cut(s) 31, 187
MalI GATC 3 cut(s) 402, 639, 1016
MboI GATC 3 cut(s) 400, 637, 1014
MboII GAAGA 7 cut(s) 307, 541, 706, 892, 1003, 1045, 1115
MhlI GDGCHC 3 cut(s) 393, 749, 836
MluCI AATT 8 cut(s) 72, 138, 494, 520, 811, 925, 936, 1162
MmeI TCCRAC 2 cut(s) 278, 744
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 3 cut(s) 422, 492, 822
MslI CAYNNNNRTG 2 cut(s) 194, 796
MspI CCGG 4 cut(s) 78, 165, 177, 223
MspR9I CCNGG 3 cut(s) 78, 178, 617
Mva1269I GAATGC 1 cut(s) 538
MvaI CCWGG 1 cut(s) 617
MvnI CGCG 1 cut(s) 1136
MwoI GCNNNNNNNGC 4 cut(s) 66, 140, 788, 1036
NciI CCSGG 2 cut(s) 78, 178
NdeII GATC 3 cut(s) 400, 637, 1014
NlaIII CATG 4 cut(s) 199, 232, 890, 917
NlaIV GGNNCC 4 cut(s) 82, 348, 392, 1096
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 31, 187
NspV TTCGAA 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 538
PdmI GAANNNNTTC 1 cut(s) 311
PfeI GAWTC 5 cut(s) 101, 721, 965, 994, 1079
PkrI GCNGC 3 cut(s) 571, 661, 757
Psp6I CCWGG 1 cut(s) 615
PspEI GGTNACC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 4 cut(s) 82, 348, 392, 1096
PspPI GGNCC 3 cut(s) 125, 225, 347
PstI CTGCAG 1 cut(s) 571
RsaI GTAC 5 cut(s) 507, 562, 776, 1073, 1096
RsaNI GTAC 5 cut(s) 506, 561, 775, 1072, 1095
RseI CAYNNNNRTG 2 cut(s) 194, 796
SaqAI TTAA 3 cut(s) 422, 492, 822
SatI GCNGC 3 cut(s) 570, 660, 756
Sau3AI GATC 3 cut(s) 400, 637, 1014
Sau96I GGNCC 3 cut(s) 125, 225, 347
ScrFI CCNGG 3 cut(s) 78, 178, 617
SduI GDGCHC 3 cut(s) 393, 749, 836
SfaNI GCATC 3 cut(s) 564, 800, 850
SfcI CTRYAG 1 cut(s) 567
SfuI TTCGAA 1 cut(s) 600
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 2 cut(s) 194, 796
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 8 cut(s) 72, 138, 494, 520, 811, 925, 936, 1162
SsiI CCGC 4 cut(s) 343, 628, 957, 1136
SspMI CTAG 1 cut(s) 171
StyD4I CCNGG 3 cut(s) 76, 176, 615
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 3 cut(s) 37, 272, 779
TaiI ACGT 1 cut(s) 635
TaqI TCGA 8 cut(s) 99, 323, 486, 600, 645, 786, 1059, 1116
TasI AATT 8 cut(s) 72, 138, 494, 520, 811, 925, 936, 1162
TatI WGTACW 1 cut(s) 774
TfiI GAWTC 5 cut(s) 101, 721, 965, 994, 1079
Tru1I TTAA 3 cut(s) 422, 492, 822
Tru9I TTAA 3 cut(s) 422, 492, 822
TscAI CASTG 6 cut(s) 275, 754, 784, 978, 1047, 1116
TseFI GTSAC 2 cut(s) 31, 187
TseI GCWGC 3 cut(s) 569, 659, 755
Tsp45I GTSAC 2 cut(s) 31, 187
TspDTI ATGAA 6 cut(s) 249, 353, 513, 824, 875, 978
TspRI CASTG 6 cut(s) 275, 754, 784, 978, 1047, 1116
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 3 cut(s) 494, 520, 936
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.