Rh2DG180600

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001289
Physical Location & Seq
Reverse (-)
15908722 .. 15914509
5788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG180600.1

Sequence Viewer

Length: 993 bp
ATGTCGATTGGTTTGGCAAAGAGAAAGATGGTGACAGTTCTGAGCATTGATGGAGGGGGCATTAGAGGCATAATTCCCAGCACCATCCTCGGCTTTCTAGAATCTAAGCTTCAGGATTTGGATGGAGCAAATGCAAGAATTGCAGATTACTTTGACATTATCGCCGGAACTAGCACTGGTGGGTTGGTCACCACCATGCTTACAGCTCCCAACAAGAACAATCGACCCATGTATGAAGCAAAGGACATCAACAACTTTTATTTAGAACACTGTCCCCAGATTTTCCCTCAAAGAAGTCGGAAAAACTTCTTCACATCGATCACAAATGCAATGAAACCGGGGCCAAAATACGATGGGAAGTATCTGCGCTCATTGATAAACGGGCTCCTTGGTGATCTCACTCTCAAGCAGACTTTAACTAATGTGGTCATTCCAAGTTTTGATATCAAACACCTTCAGCCTGTGGTTTTCTCAACCATCGATGCAAAAGAAAATTCACTGAAGAATGCAAAGCTTTCAGATGTCTGCATCAGTACCTCTGCAGCTCCATCTTATCTCCCCCCACACTACTTTGAAGTCAAGGATGACCAGGGAAATATCCGCACGTTTGATCTTGTCGATGGTGGGGTTGCTGCAAACAATCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATGTTGAAGAACAACTCAGAACCGATGGATGCGACTCGGTTGCAAGTGCTGTCATTGGGCACCGGTGCTGCCAAGTATGCAGAGAAGTACAGTGCAGTCGACGCATCAAAATGGGGTTTGGTGAATTGGATGTTTGATAATGGGAGCACACCTTTGCTGGACATTTTTGGGGATGCAAGTTCTGATATGGTTGACTTTCATGTCTCCACTCTCTTCCAGTCCCTCAATGTCAAGGACAATTACCTAAGAATTCAGGTACCTAAAATCACAGACATAACATATGTAACTGCGCTGTTGCTTACTAGACAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

330

Amino Acids

36.28

Weight (kDa)

7.71

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 220 2.4e-29 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 878
Acc65I GGTACC 1 cut(s) 934
AccB1I GGYRCC 2 cut(s) 737, 934
AccI GTMKAC 1 cut(s) 777
AciI CCGC 1 cut(s) 601
AcoI YGGCCR 1 cut(s) 654
AcsI RAATTY 2 cut(s) 493, 927
AcuI CTGAAG 3 cut(s) 95, 440, 521
AfaI GTAC 3 cut(s) 535, 767, 936
AgeI ACCGGT 1 cut(s) 740
AgsI TTSAA 2 cut(s) 575, 685
AjnI CCWGG 1 cut(s) 588
AluBI AGCT 4 cut(s) 109, 206, 514, 545
AluI AGCT 4 cut(s) 109, 206, 514, 545
Alw21I GWGCWC 1 cut(s) 827
Alw26I GTCTC 1 cut(s) 886
AoxI GGCC 2 cut(s) 341, 654
ApeKI GCWGC 3 cut(s) 542, 632, 746
ApoI RAATTY 2 cut(s) 493, 927
AsiGI ACCGGT 1 cut(s) 740
Asp700I GAANNNNTTC 1 cut(s) 305
Asp718I GGTACC 1 cut(s) 934
AspLEI GCGC 2 cut(s) 369, 970
AspS9I GGNCC 1 cut(s) 341
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 4 cut(s) 43, 181, 404, 811
BaeGI GKGCMC 1 cut(s) 740
BalI TGGCCA 1 cut(s) 656
BanI GGYRCC 2 cut(s) 737, 934
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 827
BbvI GCAGC 3 cut(s) 554, 619, 733
BcgI CGANNNNNNTGC 4 cut(s) 70, 104, 700, 734
BciT130I CCWGG 1 cut(s) 590
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 886
BfaI CTAG 3 cut(s) 98, 171, 981
BfmI CTRYAG 1 cut(s) 540
BisI GCNGC 3 cut(s) 543, 633, 747
BlsI GCNGC 3 cut(s) 544, 634, 748
Bme1390I CCNGG 2 cut(s) 339, 590
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 4 cut(s) 342, 386, 739, 936
BmrFI CCNGG 2 cut(s) 339, 590
BmsI GCATC 5 cut(s) 472, 537, 697, 791, 841
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 339
Bsa29I ATCGAT 2 cut(s) 317, 480
BsaJI CCNNGG 4 cut(s) 88, 338, 388, 589
BsaWI WCCGGW 1 cut(s) 740
Bse118I RCCGGY 1 cut(s) 740
Bse1I ACTGG 2 cut(s) 181, 895
Bse3DI GCAATG 1 cut(s) 336
BseBI CCWGG 1 cut(s) 590
BseCI ATCGAT 2 cut(s) 317, 480
BseDI CCNNGG 4 cut(s) 88, 338, 388, 589
BseGI GGATG 6 cut(s) 84, 127, 589, 712, 813, 856
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 2 cut(s) 32, 708
BseNI ACTGG 2 cut(s) 181, 895
BseSI GKGCMC 1 cut(s) 740
BseXI GCAGC 3 cut(s) 554, 619, 733
BseYI CCCAGC 1 cut(s) 77
BsgI GTGCAG 1 cut(s) 792
BshFI GGCC 2 cut(s) 343, 656
BshNI GGYRCC 2 cut(s) 737, 934
BshTI ACCGGT 1 cut(s) 740
BshVI ATCGAT 2 cut(s) 317, 480
BsiHKAI GWGCWC 1 cut(s) 827
BsiSI CCGG 3 cut(s) 165, 338, 741
BslFI GGGAC 2 cut(s) 258, 883
BsmAI GTCTC 1 cut(s) 886
BsmFI GGGAC 2 cut(s) 258, 883
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 2 cut(s) 343, 656
Bsp1286I GDGCHC 3 cut(s) 387, 740, 827
Bsp143I GATC 3 cut(s) 318, 394, 610
BspACI CCGC 1 cut(s) 601
BspANI GGCC 2 cut(s) 343, 656
BspCNI CTCAG 2 cut(s) 33, 707
BspDI ATCGAT 2 cut(s) 317, 480
BspLI GGNNCC 4 cut(s) 342, 386, 739, 936
BspMAI CTGCAG 1 cut(s) 544
BspT107I GGYRCC 2 cut(s) 737, 934
BsrDI GCAATG 1 cut(s) 336
BsrFI RCCGGY 1 cut(s) 740
BsrI ACTGG 2 cut(s) 181, 895
BssAI RCCGGY 1 cut(s) 740
BssECI CCNNGG 4 cut(s) 88, 338, 388, 589
BssMI GATC 3 cut(s) 318, 394, 610
BssT1I CCWWGG 1 cut(s) 388
Bst2UI CCWGG 1 cut(s) 590
Bst4CI ACNGT 3 cut(s) 37, 272, 770
Bst6I CTCTTC 1 cut(s) 896
BstAPI GCANNNNNTGC 1 cut(s) 140
BstDEI CTNAG 4 cut(s) 41, 105, 694, 923
BstEII GGTNACC 1 cut(s) 187
BstF5I GGATG 6 cut(s) 84, 127, 589, 712, 813, 856
BstHHI GCGC 2 cut(s) 369, 970
BstKTI GATC 3 cut(s) 321, 397, 613
BstMAI GTCTC 1 cut(s) 886
BstMBI GATC 3 cut(s) 318, 394, 610
BstMWI GCNNNNNNNGC 4 cut(s) 66, 140, 755, 779
BstNI CCWGG 1 cut(s) 590
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 2 cut(s) 337, 588
BstSFI CTRYAG 1 cut(s) 540
BstSLI GKGCMC 1 cut(s) 740
BstV1I GCAGC 3 cut(s) 554, 619, 733
Bsu15I ATCGAT 2 cut(s) 317, 480
BsuRI GGCC 2 cut(s) 343, 656
BsuTUI ATCGAT 2 cut(s) 317, 480
BtsCI GGATG 6 cut(s) 84, 127, 589, 712, 813, 856
BtsIMutI CAGTG 4 cut(s) 174, 268, 497, 775
CfoI GCGC 2 cut(s) 369, 970
Cfr10I RCCGGY 1 cut(s) 740
Cfr13I GGNCC 1 cut(s) 341
ClaI ATCGAT 2 cut(s) 317, 480
CseI GACGC 1 cut(s) 788
Csp6I GTAC 3 cut(s) 534, 766, 935
CspAI ACCGGT 1 cut(s) 740
CviAII CATG 3 cut(s) 196, 229, 878
CviQI GTAC 3 cut(s) 534, 766, 935
DdeI CTNAG 4 cut(s) 41, 105, 694, 923
DpnI GATC 3 cut(s) 320, 396, 612
DpnII GATC 3 cut(s) 318, 394, 610
DrdI GACNNNNNNGTC 1 cut(s) 878
DseDI GACNNNNNNGTC 1 cut(s) 878
EaeI YGGCCR 1 cut(s) 654
Eam1104I CTCTTC 1 cut(s) 896
EarI CTCTTC 1 cut(s) 896
Eco130I CCWWGG 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 387
Eco32I GATATC 1 cut(s) 445
Eco57I CTGAAG 3 cut(s) 95, 440, 521
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
EcoRI GAATTC 1 cut(s) 927
EcoRII CCWGG 1 cut(s) 588
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 388
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 3 cut(s) 199, 232, 881
FaqI GGGAC 2 cut(s) 258, 883
FatI CATG 3 cut(s) 195, 228, 877
FauNDI CATATG 1 cut(s) 958
FblI GTMKAC 1 cut(s) 777
Fnu4HI GCNGC 3 cut(s) 543, 633, 747
FokI GGATG 6 cut(s) 71, 134, 596, 719, 820, 863
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 3 cut(s) 543, 633, 747
FspBI CTAG 3 cut(s) 98, 171, 981
GlaI GCGC 2 cut(s) 368, 969
GluI GCNGC 3 cut(s) 543, 633, 747
GsaI CCCAGC 1 cut(s) 81
HaeIII GGCC 2 cut(s) 343, 656
HapII CCGG 3 cut(s) 165, 338, 741
HgaI GACGC 1 cut(s) 788
HhaI GCGC 2 cut(s) 369, 970
Hin1II CATG 3 cut(s) 199, 232, 881
Hin6I GCGC 2 cut(s) 367, 968
HinP1I GCGC 2 cut(s) 367, 968
HincII GTYRAC 2 cut(s) 778, 871
HindII GTYRAC 2 cut(s) 778, 871
HindIII AAGCTT 2 cut(s) 107, 512
HinfI GANTC 2 cut(s) 101, 712
HpaII CCGG 3 cut(s) 165, 338, 741
HphI GGTGA 4 cut(s) 43, 181, 404, 811
Hpy166II GTNNAC 2 cut(s) 778, 871
Hpy188I TCNGA 5 cut(s) 42, 300, 520, 697, 862
Hpy188III TCNNGA 2 cut(s) 98, 113
Hpy8I GTNNAC 2 cut(s) 778, 871
Hpy99I CGWCG 1 cut(s) 782
HpyAV CCTTC 1 cut(s) 464
HpyCH4III ACNGT 3 cut(s) 37, 272, 770
HpyCH4IV ACGT 1 cut(s) 605
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 140, 755, 779
HpyF3I CTNAG 4 cut(s) 41, 105, 694, 923
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 3 cut(s) 199, 232, 881
HspAI GCGC 2 cut(s) 367, 968
KpnI GGTACC 1 cut(s) 938
Kzo9I GATC 3 cut(s) 318, 394, 610
LmnI GCTCC 5 cut(s) 125, 211, 390, 550, 822
Lsp1109I GCAGC 3 cut(s) 554, 619, 733
LweI GCATC 5 cut(s) 472, 537, 697, 791, 841
MaeI CTAG 3 cut(s) 98, 171, 981
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 3 cut(s) 31, 187, 961
MalI GATC 3 cut(s) 320, 396, 612
MboI GATC 3 cut(s) 318, 394, 610
MboII GAAGA 4 cut(s) 301, 514, 697, 883
MhlI GDGCHC 3 cut(s) 387, 740, 827
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 6 cut(s) 72, 138, 493, 802, 916, 927
MluNI TGGCCA 1 cut(s) 656
MlyI GAGTC 1 cut(s) 706
MmeI TCCRAC 1 cut(s) 278
MnlI CCTC 6 cut(s) 47, 59, 98, 297, 547, 911
Mox20I TGGCCA 1 cut(s) 656
MroXI GAANNNNTTC 1 cut(s) 305
MscI TGGCCA 1 cut(s) 656
MseI TTAA 1 cut(s) 416
MslI CAYNNNNRTG 2 cut(s) 194, 787
Msp20I TGGCCA 1 cut(s) 656
MspI CCGG 3 cut(s) 165, 338, 741
MspR9I CCNGG 2 cut(s) 339, 590
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 1 cut(s) 590
MwoI GCNNNNNNNGC 4 cut(s) 66, 140, 755, 779
NciI CCSGG 1 cut(s) 339
NdeI CATATG 1 cut(s) 958
NdeII GATC 3 cut(s) 318, 394, 610
NlaIII CATG 3 cut(s) 199, 232, 881
NlaIV GGNNCC 4 cut(s) 342, 386, 739, 936
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 31, 187
PctI GAATGC 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 1 cut(s) 101
PinAI ACCGGT 1 cut(s) 740
PkrI GCNGC 3 cut(s) 544, 634, 748
PleI GAGTC 1 cut(s) 706
PpsI GAGTC 1 cut(s) 706
Psp6I CCWGG 1 cut(s) 588
PspEI GGTNACC 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 1 cut(s) 588
PspN4I GGNNCC 4 cut(s) 342, 386, 739, 936
PspPI GGNCC 1 cut(s) 341
PstI CTGCAG 1 cut(s) 544
RsaI GTAC 3 cut(s) 535, 767, 936
RsaNI GTAC 3 cut(s) 534, 766, 935
RseI CAYNNNNRTG 2 cut(s) 194, 787
SalI GTCGAC 1 cut(s) 776
SaqAI TTAA 1 cut(s) 416
SatI GCNGC 3 cut(s) 543, 633, 747
Sau3AI GATC 3 cut(s) 318, 394, 610
Sau96I GGNCC 1 cut(s) 341
SchI GAGTC 1 cut(s) 706
ScrFI CCNGG 2 cut(s) 339, 590
SduI GDGCHC 3 cut(s) 387, 740, 827
SfaNI GCATC 5 cut(s) 472, 537, 697, 791, 841
SfcI CTRYAG 1 cut(s) 540
SgrAI CRCCGGYG 1 cut(s) 740
SmiMI CAYNNNNRTG 2 cut(s) 194, 787
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 6 cut(s) 72, 138, 493, 802, 916, 927
SsiI CCGC 1 cut(s) 601
SspMI CTAG 3 cut(s) 98, 171, 981
StyD4I CCNGG 2 cut(s) 337, 588
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 3 cut(s) 37, 272, 770
TaiI ACGT 1 cut(s) 608
TaqI TCGA 6 cut(s) 5, 223, 317, 480, 618, 777
TasI AATT 6 cut(s) 72, 138, 493, 802, 916, 927
TatI WGTACW 1 cut(s) 765
TfiI GAWTC 1 cut(s) 101
Tru1I TTAA 1 cut(s) 416
Tru9I TTAA 1 cut(s) 416
TscAI CASTG 4 cut(s) 181, 275, 504, 775
TseFI GTSAC 2 cut(s) 31, 187
TseI GCWGC 3 cut(s) 542, 632, 746
Tsp45I GTSAC 2 cut(s) 31, 187
TspDTI ATGAA 3 cut(s) 249, 347, 866
TspRI CASTG 4 cut(s) 181, 275, 504, 775
XapI RAATTY 2 cut(s) 493, 927
XbaI TCTAGA 1 cut(s) 97
XmiI GTMKAC 1 cut(s) 777
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 3 cut(s) 98, 171, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.