RLG00000017368

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001289
Physical Location & Seq
Reverse (-)
15784681 .. 15786647
1967 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017368

Sequence Viewer

Length: 1131 bp
ATGGTGACAGTTCTGAGCATTGATGGAGGGGGCATTAGAGGCATAATTCCCAGCACCATCCTCGGCTTTCTCGAATCTAAGCTTCAGGATTTGGATGGAGCAAATGCAAGAATTGCAGATTACTTTGACATTATCGCCGGAACTAGCACCGGTGGGTTGGTCACCACCATGCTTACAGCTCCCAACAAGAACAATCGACCCATGTATGAAGCCAAGGACATCAACAACTTTTATTTAGAACACTGTCCCCAGATTTTCCCTCAAAAAAGTCGGAAAAACTTCGTCACATCGATCACAAATGCAATGAAACCGGGGCCAAAATACGATGGGAAGTATCTGCGCTCATTGACAAACGGGCTGCTTGGTGACCTCACTCTCAAGCAGACTTTAACCAATGTGGTGATTCCAACTTTTGATATCAAACACCTTCAGCCTGTGGTTTTCACAACCATCGACGCAAAAGAAAATTCATTGAAGAACGCAAAGCTTTCAGATGTCTGCATCAGTACCTCTGCAGCTCCATCTTATCTTCCCCCACACTACTTTGAAGTCAAGGATGACCAGGGAAATATCCGCACGTTTGATCTTGTCGATGGTGGGGTTGCTGCAAACAATCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATGTTGAAGAACAACTCAGAACCGATGGATGCGACTCGGTTGCAAGTGCTGTCACTGGGCACCGGTGCTGCCAAGTATGCAGAGAAGTACAGTGCAGTCGACGCATCAAAATGGGGTTTGGTGAATTGGATGTTTGATAATGGGAGCACACCTTTGCCGGACATTTTTGGCGAAGCAAGTTCTGATATGGTTGACTTTCATGTCTCCACTCTCTTCCAGTCCCTCAATGTCAAGGACAATTACCTAAGAATTCAGGATGACACATTGAGCGGTGAAGAATCATCAGTGGATATTGCAACCGAGAAGAATCTGGAAAGCCTTGTGGTGATCGGAAACGGGTTGCTAAAGAAGCCAGTGTCAAGGGTGAATATGGATACAGGGAGGTATGAGAAATGTGAGGGAGAAGCCACCTATGAAGAAGCACTAGTCCATTTTGCCATACAGCTCTCGGAGGGAAGGAAGCTCAGACAAACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

377

Amino Acids

41.32

Weight (kDa)

5.37

Isoelectric Point (pI)

29.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 5 - 211 3.6e-28 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 851
AccB1I GGYRCC 1 cut(s) 710
AccBSI CCGCTC 1 cut(s) 921
AccI GTMKAC 1 cut(s) 750
AciI CCGC 2 cut(s) 574, 921
AcoI YGGCCR 1 cut(s) 627
AcsI RAATTY 2 cut(s) 466, 900
AcuI CTGAAG 2 cut(s) 68, 413
AfaI GTAC 2 cut(s) 508, 740
AgeI ACCGGT 2 cut(s) 149, 713
AgsI TTSAA 3 cut(s) 475, 548, 658
AhlI ACTAGT 1 cut(s) 1075
AjnI CCWGG 1 cut(s) 561
AluBI AGCT 6 cut(s) 82, 179, 487, 518, 1096, 1114
AluI AGCT 6 cut(s) 82, 179, 487, 518, 1096, 1114
Alw21I GWGCWC 1 cut(s) 800
Alw26I GTCTC 1 cut(s) 859
AoxI GGCC 2 cut(s) 314, 627
ApeKI GCWGC 4 cut(s) 358, 515, 605, 719
ApoI RAATTY 2 cut(s) 466, 900
AsiGI ACCGGT 2 cut(s) 149, 713
Asp700I GAANNNNTTC 1 cut(s) 278
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 1 cut(s) 314
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 8 cut(s) 16, 154, 377, 412, 784, 935, 988, 1027
BaeGI GKGCMC 1 cut(s) 713
BalI TGGCCA 1 cut(s) 629
BanI GGYRCC 1 cut(s) 710
Bbv12I GWGCWC 1 cut(s) 800
BbvI GCAGC 4 cut(s) 345, 527, 592, 706
BccI CCATC 9 cut(s) 17, 65, 89, 320, 458, 529, 587, 619, 670
BcgI CGANNNNNNTGC 4 cut(s) 43, 77, 673, 707
BciT130I CCWGG 1 cut(s) 563
BciVI GTATCC 1 cut(s) 1018
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 1 cut(s) 859
BcuI ACTAGT 1 cut(s) 1075
BfaI CTAG 2 cut(s) 144, 1076
BfmI CTRYAG 1 cut(s) 513
BfuI GTATCC 1 cut(s) 1018
BisI GCNGC 4 cut(s) 359, 516, 606, 720
BlsI GCNGC 4 cut(s) 360, 517, 607, 721
Bme1390I CCNGG 2 cut(s) 312, 563
BmgT120I GGNCC 1 cut(s) 314
BmiI GGNNCC 2 cut(s) 315, 712
BmrFI CCNGG 2 cut(s) 312, 563
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 3 cut(s) 510, 670, 764
BmuI ACTGGG 1 cut(s) 716
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 312
Bsa29I ATCGAT 1 cut(s) 290
BsaJI CCNNGG 4 cut(s) 61, 213, 311, 562
BsaWI WCCGGW 2 cut(s) 149, 713
Bse118I RCCGGY 2 cut(s) 149, 713
Bse1I ACTGG 3 cut(s) 711, 868, 1004
Bse3DI GCAATG 1 cut(s) 309
BseBI CCWGG 1 cut(s) 563
BseCI ATCGAT 1 cut(s) 290
BseDI CCNNGG 4 cut(s) 61, 213, 311, 562
BseGI GGATG 6 cut(s) 57, 100, 562, 685, 786, 913
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 3 cut(s) 5, 681, 1129
BseNI ACTGG 3 cut(s) 711, 868, 1004
BseSI GKGCMC 1 cut(s) 713
BseXI GCAGC 4 cut(s) 345, 527, 592, 706
BseYI CCCAGC 1 cut(s) 50
BsgI GTGCAG 1 cut(s) 765
BshFI GGCC 2 cut(s) 316, 629
BshNI GGYRCC 1 cut(s) 710
BshTI ACCGGT 2 cut(s) 149, 713
BshVI ATCGAT 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 800
BsiSI CCGG 5 cut(s) 138, 150, 311, 714, 809
BslFI GGGAC 2 cut(s) 231, 856
BsmAI GTCTC 1 cut(s) 859
BsmFI GGGAC 2 cut(s) 231, 856
BsnI GGCC 2 cut(s) 316, 629
Bsp1286I GDGCHC 2 cut(s) 713, 800
Bsp143I GATC 3 cut(s) 291, 583, 978
BspACI CCGC 2 cut(s) 574, 921
BspANI GGCC 2 cut(s) 316, 629
BspCNI CTCAG 3 cut(s) 6, 680, 1128
BspDI ATCGAT 1 cut(s) 290
BspLI GGNNCC 2 cut(s) 315, 712
BspMAI CTGCAG 1 cut(s) 517
BspT107I GGYRCC 1 cut(s) 710
BsrBI CCGCTC 1 cut(s) 921
BsrDI GCAATG 1 cut(s) 309
BsrFI RCCGGY 2 cut(s) 149, 713
BsrI ACTGG 3 cut(s) 711, 868, 1004
BssAI RCCGGY 2 cut(s) 149, 713
BssECI CCNNGG 4 cut(s) 61, 213, 311, 562
BssMI GATC 3 cut(s) 291, 583, 978
BssT1I CCWWGG 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 3 cut(s) 10, 245, 743
Bst6I CTCTTC 1 cut(s) 869
BstAPI GCANNNNNTGC 1 cut(s) 113
BstDEI CTNAG 5 cut(s) 14, 78, 667, 896, 1115
BstEII GGTNACC 2 cut(s) 160, 365
BstF5I GGATG 6 cut(s) 57, 100, 562, 685, 786, 913
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 3 cut(s) 294, 586, 981
BstMAI GTCTC 1 cut(s) 859
BstMBI GATC 3 cut(s) 291, 583, 978
BstMWI GCNNNNNNNGC 5 cut(s) 39, 113, 728, 752, 1000
BstNI CCWGG 1 cut(s) 563
BstPI GGTNACC 2 cut(s) 160, 365
BstSCI CCNGG 2 cut(s) 310, 561
BstSFI CTRYAG 1 cut(s) 513
BstSLI GKGCMC 1 cut(s) 713
BstV1I GCAGC 4 cut(s) 345, 527, 592, 706
Bsu15I ATCGAT 1 cut(s) 290
BsuI GTATCC 1 cut(s) 1018
BsuRI GGCC 2 cut(s) 316, 629
BsuTUI ATCGAT 1 cut(s) 290
BtsCI GGATG 6 cut(s) 57, 100, 562, 685, 786, 913
BtsIMutI CAGTG 5 cut(s) 241, 704, 748, 942, 1011
CfoI GCGC 1 cut(s) 342
Cfr10I RCCGGY 2 cut(s) 149, 713
Cfr13I GGNCC 1 cut(s) 314
ClaI ATCGAT 1 cut(s) 290
CseI GACGC 2 cut(s) 464, 761
Csp6I GTAC 2 cut(s) 507, 739
CspAI ACCGGT 2 cut(s) 149, 713
CviAII CATG 3 cut(s) 169, 202, 851
CviQI GTAC 2 cut(s) 507, 739
DdeI CTNAG 5 cut(s) 14, 78, 667, 896, 1115
DpnI GATC 3 cut(s) 293, 585, 980
DpnII GATC 3 cut(s) 291, 583, 978
DrdI GACNNNNNNGTC 1 cut(s) 851
DseDI GACNNNNNNGTC 1 cut(s) 851
EaeI YGGCCR 1 cut(s) 627
Eam1104I CTCTTC 1 cut(s) 869
EarI CTCTTC 1 cut(s) 869
Eco130I CCWWGG 1 cut(s) 213
Eco32I GATATC 1 cut(s) 418
Eco57I CTGAAG 2 cut(s) 68, 413
Eco91I GGTNACC 2 cut(s) 160, 365
EcoO65I GGTNACC 2 cut(s) 160, 365
EcoRI GAATTC 1 cut(s) 900
EcoRII CCWGG 1 cut(s) 561
EcoRV GATATC 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 3 cut(s) 172, 205, 854
FaqI GGGAC 2 cut(s) 231, 856
FatI CATG 3 cut(s) 168, 201, 850
FblI GTMKAC 1 cut(s) 750
Fnu4HI GCNGC 4 cut(s) 359, 516, 606, 720
FokI GGATG 6 cut(s) 44, 107, 569, 692, 793, 920
Fsp4HI GCNGC 4 cut(s) 359, 516, 606, 720
FspBI CTAG 2 cut(s) 144, 1076
GlaI GCGC 1 cut(s) 341
GluI GCNGC 4 cut(s) 359, 516, 606, 720
GsaI CCCAGC 1 cut(s) 54
HaeIII GGCC 2 cut(s) 316, 629
HapII CCGG 5 cut(s) 138, 150, 311, 714, 809
HgaI GACGC 2 cut(s) 464, 761
HhaI GCGC 1 cut(s) 342
Hin1II CATG 3 cut(s) 172, 205, 854
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HincII GTYRAC 2 cut(s) 751, 844
HindII GTYRAC 2 cut(s) 751, 844
HindIII AAGCTT 2 cut(s) 80, 485
HinfI GANTC 5 cut(s) 74, 403, 685, 929, 958
HpaII CCGG 5 cut(s) 138, 150, 311, 714, 809
HphI GGTGA 8 cut(s) 16, 154, 377, 412, 784, 935, 988, 1027
Hpy166II GTNNAC 2 cut(s) 751, 844
Hpy188I TCNGA 8 cut(s) 15, 273, 493, 670, 835, 983, 1102, 1118
Hpy188III TCNNGA 4 cut(s) 71, 86, 905, 962
Hpy8I GTNNAC 2 cut(s) 751, 844
Hpy99I CGWCG 2 cut(s) 458, 755
HpyAV CCTTC 2 cut(s) 437, 1101
HpyCH4III ACNGT 3 cut(s) 10, 245, 743
HpyCH4IV ACGT 1 cut(s) 578
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 113, 728, 752, 1000
HpyF3I CTNAG 5 cut(s) 14, 78, 667, 896, 1115
HpySE526I ACGT 1 cut(s) 578
Hsp92II CATG 3 cut(s) 172, 205, 854
HspAI GCGC 1 cut(s) 340
Kzo9I GATC 3 cut(s) 291, 583, 978
LmnI GCTCC 4 cut(s) 98, 184, 523, 795
Lsp1109I GCAGC 4 cut(s) 345, 527, 592, 706
LweI GCATC 3 cut(s) 510, 670, 764
MaeI CTAG 2 cut(s) 144, 1076
MaeII ACGT 1 cut(s) 578
MaeIII GTNAC 5 cut(s) 4, 160, 283, 365, 702
MalI GATC 3 cut(s) 293, 585, 980
MbiI CCGCTC 1 cut(s) 921
MboI GATC 3 cut(s) 291, 583, 978
MboII GAAGA 7 cut(s) 487, 521, 670, 856, 938, 967, 1079
MfeI CAATTG 1 cut(s) 1126
MhlI GDGCHC 2 cut(s) 713, 800
MlsI TGGCCA 1 cut(s) 629
MluCI AATT 7 cut(s) 45, 111, 466, 775, 889, 900, 1126
MluNI TGGCCA 1 cut(s) 629
MlyI GAGTC 1 cut(s) 679
MmeI TCCRAC 2 cut(s) 251, 431
Mox20I TGGCCA 1 cut(s) 629
MroXI GAANNNNTTC 1 cut(s) 278
MscI TGGCCA 1 cut(s) 629
MseI TTAA 1 cut(s) 389
MslI CAYNNNNRTG 2 cut(s) 167, 760
Msp20I TGGCCA 1 cut(s) 629
MspI CCGG 5 cut(s) 138, 150, 311, 714, 809
MspR9I CCNGG 2 cut(s) 312, 563
MunI CAATTG 1 cut(s) 1126
MvaI CCWGG 1 cut(s) 563
MwoI GCNNNNNNNGC 5 cut(s) 39, 113, 728, 752, 1000
NciI CCSGG 1 cut(s) 312
NdeII GATC 3 cut(s) 291, 583, 978
NlaIII CATG 3 cut(s) 172, 205, 854
NlaIV GGNNCC 2 cut(s) 315, 712
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 5 cut(s) 4, 160, 283, 365, 702
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 4 cut(s) 74, 403, 929, 958
PinAI ACCGGT 2 cut(s) 149, 713
PkrI GCNGC 4 cut(s) 360, 517, 607, 721
PleI GAGTC 1 cut(s) 679
PpsI GAGTC 1 cut(s) 679
Psp6I CCWGG 1 cut(s) 561
PspEI GGTNACC 2 cut(s) 160, 365
PspFI CCCAGC 1 cut(s) 50
PspGI CCWGG 1 cut(s) 561
PspN4I GGNNCC 2 cut(s) 315, 712
PspPI GGNCC 1 cut(s) 314
PstI CTGCAG 1 cut(s) 517
RsaI GTAC 2 cut(s) 508, 740
RsaNI GTAC 2 cut(s) 507, 739
RseI CAYNNNNRTG 2 cut(s) 167, 760
SalI GTCGAC 1 cut(s) 749
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 4 cut(s) 359, 516, 606, 720
Sau3AI GATC 3 cut(s) 291, 583, 978
Sau96I GGNCC 1 cut(s) 314
SchI GAGTC 1 cut(s) 679
ScrFI CCNGG 2 cut(s) 312, 563
SduI GDGCHC 2 cut(s) 713, 800
SfaNI GCATC 3 cut(s) 510, 670, 764
SfcI CTRYAG 1 cut(s) 513
SgrAI CRCCGGYG 2 cut(s) 149, 713
SmiMI CAYNNNNRTG 2 cut(s) 167, 760
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SpeI ACTAGT 1 cut(s) 1075
Sse9I AATT 7 cut(s) 45, 111, 466, 775, 889, 900, 1126
SsiI CCGC 2 cut(s) 574, 921
SspMI CTAG 2 cut(s) 144, 1076
StyD4I CCNGG 2 cut(s) 310, 561
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 3 cut(s) 10, 245, 743
TaiI ACGT 1 cut(s) 581
TaqI TCGA 6 cut(s) 72, 196, 290, 453, 591, 750
TasI AATT 7 cut(s) 45, 111, 466, 775, 889, 900, 1126
TatI WGTACW 1 cut(s) 738
TfiI GAWTC 4 cut(s) 74, 403, 929, 958
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TscAI CASTG 5 cut(s) 248, 711, 748, 942, 1011
TseFI GTSAC 5 cut(s) 4, 160, 283, 365, 702
TseI GCWGC 4 cut(s) 358, 515, 605, 719
Tsp45I GTSAC 5 cut(s) 4, 160, 283, 365, 702
TspDTI ATGAA 5 cut(s) 222, 320, 459, 839, 1080
TspRI CASTG 5 cut(s) 248, 711, 748, 942, 1011
XapI RAATTY 2 cut(s) 466, 900
XmiI GTMKAC 1 cut(s) 750
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 2 cut(s) 144, 1076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.