Rmu_sc0002645.1_g000013

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002645.1
OG0001289
Physical Location & Seq
Forward (+)
57253 .. 59334
2082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002645.1_g000013.1.cds

Sequence Viewer

Length: 1128 bp
atggtgacagtgctaagcattgatggaggtggaattaaaggaatcatacccggcacccttcttgcctttcttgaaaccaagcttcaggatttggatggtccgagtgcaagacttgcagattattttgatataattgccggaacaagcacaggggggttggtcactaccatgcttacagctcccaacaaggacaatcgacccatgtatgaagcaaaggacatcaacaacttctactttgaacaaacacccaagatcttccctcaaaatagtcaaaaaaacttcttgacatcgattcaaagtgcggttggtgccgtgatgggcccaaaatacgatgggaagtacctgcgtacaatgacgaacaagctgctcggtgaccttactctcaagcaaactctaaccaatgtgatcatcccagcctttgatatcaaacaccttcagccagtgattttctcaaccatcgatgcaaaagaaagttctctgaaatatccaaagctttcggatatctgtatcagtacctctgcagctccaacttttctccccgcacactacttcgaggtcaaggacagccagggaacaacccgtacttttgatctcgtcgatggtggggttgccgcaaacaaccctacaatgatggccataagccacataaacagagagatgttgaagcacggctcagaaccaatggatgctaatcgtttgctagtgctatcattgggcacaggcactgccaagttcgaagggaagtacagtgcaaccacggcctcaaaatggggtttgatcaattggatgtttgacaatgggagcacacctatgatcgatatctttaccgatgcaagttcggatatggttgacattcatgtctccactctctttcagtccctcaatgccaaagacaactacctacgtattcaggatgacagtttgatcggtgaggagtcatcggtggatattgcaacggagaaaaatcttaagaggcttgtggagatcgggaatgggctgttgagaaagccggtgtcaagggtaaatttggataacgggaggtatgagaaatctgaaggagaaggtacctatgaagaagcactggtcgattttgccaaacggctcgtggaggggaagaagcacaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

375

Amino Acids

41.21

Weight (kDa)

5.77

Isoelectric Point (pI)

30.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 857
Acc36I ACCTGC 1 cut(s) 351
Acc65I GGTACC 1 cut(s) 1064
AccB1I GGYRCC 3 cut(s) 53, 308, 1064
AciI CCGC 3 cut(s) 302, 540, 612
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 1 cut(s) 1024
AcuI CTGAAG 3 cut(s) 68, 419, 1074
AfaI GTAC 6 cut(s) 341, 349, 514, 583, 746, 1066
AfiI CCNNNNNNNGG 1 cut(s) 768
AflII CTTAAG 1 cut(s) 968
AgsI TTSAA 4 cut(s) 74, 239, 296, 664
AjnI CCWGG 1 cut(s) 567
AluBI AGCT 5 cut(s) 82, 179, 364, 493, 524
AluI AGCT 5 cut(s) 82, 179, 364, 493, 524
Alw21I GWGCWC 1 cut(s) 806
Alw26I GTCTC 1 cut(s) 865
AlwNI CAGNNNCTG 1 cut(s) 725
AoxI GGCC 3 cut(s) 319, 633, 759
ApaI GGGCCC 1 cut(s) 323
ApeKI GCWGC 2 cut(s) 364, 521
ApoI RAATTY 1 cut(s) 1024
Asp718I GGTACC 1 cut(s) 1064
AspS9I GGNCC 3 cut(s) 98, 319, 320
AsuC2I CCSGG 1 cut(s) 51
AsuHPI GGTGA 3 cut(s) 16, 383, 941
AsuII TTCGAA 1 cut(s) 735
AvaII GGWCC 1 cut(s) 98
BaeGI GKGCMC 2 cut(s) 323, 719
BalI TGGCCA 1 cut(s) 635
BanI GGYRCC 3 cut(s) 53, 308, 1064
BanII GRGCYC 1 cut(s) 323
BauI CACGAG 1 cut(s) 1103
Bbv12I GWGCWC 1 cut(s) 806
BbvI GCAGC 2 cut(s) 351, 533
BccI CCATC 7 cut(s) 17, 89, 310, 326, 464, 593, 625
BceAI ACGGC 4 cut(s) 296, 685, 774, 1115
BcgI CGANNNNNNTGC 2 cut(s) 346, 380
BciT130I CCWGG 1 cut(s) 569
BclI TGATCA 2 cut(s) 405, 777
BcnI CCSGG 1 cut(s) 51
BcoDI GTCTC 1 cut(s) 865
BfaI CTAG 1 cut(s) 701
BfmI CTRYAG 1 cut(s) 519
BfrI CTTAAG 1 cut(s) 968
BfuAI ACCTGC 1 cut(s) 351
BglII AGATCT 1 cut(s) 252
BisI GCNGC 3 cut(s) 365, 522, 612
BlpI GCTNAGC 1 cut(s) 14
BlsI GCNGC 3 cut(s) 366, 523, 613
Bme1390I CCNGG 2 cut(s) 51, 569
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 3 cut(s) 98, 319, 320
BmiI GGNNCC 4 cut(s) 55, 310, 321, 1066
BmrFI CCNGG 2 cut(s) 51, 569
BmsI GCATC 3 cut(s) 451, 676, 820
Bpu1102I GCTNAGC 1 cut(s) 14
Bpu14I TTCGAA 1 cut(s) 735
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 51
Bsa29I ATCGAT 3 cut(s) 290, 459, 816
BsaAI YACGTR 1 cut(s) 905
BsaBI GATNNNNATC 2 cut(s) 690, 818
BsaJI CCNNGG 2 cut(s) 568, 756
Bsc4I CCNNNNNNNGG 1 cut(s) 768
Bse118I RCCGGY 1 cut(s) 1009
Bse1I ACTGG 2 cut(s) 440, 1086
Bse8I GATNNNNATC 2 cut(s) 690, 818
BseBI CCWGG 1 cut(s) 569
BseCI ATCGAT 3 cut(s) 290, 459, 816
BseDI CCNNGG 2 cut(s) 568, 756
BseGI GGATG 5 cut(s) 100, 408, 691, 792, 919
BseJI GATNNNNATC 2 cut(s) 690, 818
BseLI CCNNNNNNNGG 1 cut(s) 768
BseMII CTCAG 1 cut(s) 687
BseNI ACTGG 2 cut(s) 440, 1086
BseRI GAGGAG 1 cut(s) 947
BseSI GKGCMC 2 cut(s) 323, 719
BseXI GCAGC 2 cut(s) 351, 533
BseYI CCCAGC 1 cut(s) 412
BshFI GGCC 3 cut(s) 321, 635, 761
BshNI GGYRCC 3 cut(s) 53, 308, 1064
BshVI ATCGAT 3 cut(s) 290, 459, 816
BsiHKAI GWGCWC 1 cut(s) 806
BsiSI CCGG 3 cut(s) 51, 138, 1010
BslFI GGGAC 1 cut(s) 862
BslI CCNNNNNNNGG 1 cut(s) 768
BsmAI GTCTC 1 cut(s) 865
BsmFI GGGAC 1 cut(s) 862
BsnI GGCC 3 cut(s) 321, 635, 761
Bsp119I TTCGAA 1 cut(s) 735
Bsp120I GGGCCC 1 cut(s) 319
Bsp1286I GDGCHC 3 cut(s) 323, 719, 806
Bsp143I GATC 7 cut(s) 252, 405, 589, 777, 813, 924, 984
Bsp1720I GCTNAGC 1 cut(s) 14
BspACI CCGC 3 cut(s) 302, 540, 612
BspANI GGCC 3 cut(s) 321, 635, 761
BspCNI CTCAG 1 cut(s) 686
BspDI ATCGAT 3 cut(s) 290, 459, 816
BspLI GGNNCC 4 cut(s) 55, 310, 321, 1066
BspMAI CTGCAG 1 cut(s) 523
BspMI ACCTGC 1 cut(s) 351
BspT104I TTCGAA 1 cut(s) 735
BspT107I GGYRCC 3 cut(s) 53, 308, 1064
BspTI CTTAAG 1 cut(s) 968
BsrFI RCCGGY 1 cut(s) 1009
BsrI ACTGG 2 cut(s) 440, 1086
BssAI RCCGGY 1 cut(s) 1009
BssECI CCNNGG 2 cut(s) 568, 756
BssMI GATC 7 cut(s) 252, 405, 589, 777, 813, 924, 984
BssSI CACGAG 1 cut(s) 1103
Bst2BI CACGAG 1 cut(s) 1103
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 3 cut(s) 10, 749, 920
BstAFI CTTAAG 1 cut(s) 968
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 905
BstBI TTCGAA 1 cut(s) 735
BstDEI CTNAG 2 cut(s) 14, 673
BstDSI CCRYGG 1 cut(s) 756
BstEII GGTNACC 1 cut(s) 371
BstF5I GGATG 5 cut(s) 100, 408, 691, 792, 919
BstKTI GATC 7 cut(s) 255, 408, 592, 780, 816, 927, 987
BstMAI GTCTC 1 cut(s) 865
BstMBI GATC 7 cut(s) 252, 405, 589, 777, 813, 924, 984
BstMWI GCNNNNNNNGC 3 cut(s) 113, 308, 758
BstNI CCWGG 1 cut(s) 569
BstPI GGTNACC 1 cut(s) 371
BstSCI CCNGG 2 cut(s) 49, 567
BstSFI CTRYAG 1 cut(s) 519
BstSLI GKGCMC 2 cut(s) 323, 719
BstSNI TACGTA 1 cut(s) 905
BstV1I GCAGC 2 cut(s) 351, 533
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
Bsu15I ATCGAT 3 cut(s) 290, 459, 816
BsuRI GGCC 3 cut(s) 321, 635, 761
BsuTUI ATCGAT 3 cut(s) 290, 459, 816
BtgI CCRYGG 1 cut(s) 756
BtsCI GGATG 5 cut(s) 100, 408, 691, 792, 919
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 5 cut(s) 15, 447, 723, 754, 1079
BveI ACCTGC 1 cut(s) 351
CaiI CAGNNNCTG 1 cut(s) 725
Cfr10I RCCGGY 1 cut(s) 1009
Cfr13I GGNCC 3 cut(s) 98, 319, 320
ClaI ATCGAT 3 cut(s) 290, 459, 816
Csp6I GTAC 6 cut(s) 340, 348, 513, 582, 745, 1065
CviAII CATG 3 cut(s) 169, 202, 857
CviQI GTAC 6 cut(s) 340, 348, 513, 582, 745, 1065
DdeI CTNAG 2 cut(s) 14, 673
DpnI GATC 7 cut(s) 254, 407, 591, 779, 815, 926, 986
DpnII GATC 7 cut(s) 252, 405, 589, 777, 813, 924, 984
DrdI GACNNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 1 cut(s) 633
Eco105I TACGTA 1 cut(s) 905
Eco24I GRGCYC 1 cut(s) 323
Eco32I GATATC 3 cut(s) 424, 502, 820
Eco47I GGWCC 1 cut(s) 98
Eco57I CTGAAG 3 cut(s) 68, 419, 1074
Eco91I GGTNACC 1 cut(s) 371
EcoO65I GGTNACC 1 cut(s) 371
EcoRII CCWGG 1 cut(s) 567
EcoRV GATATC 3 cut(s) 424, 502, 820
EcoT38I GRGCYC 1 cut(s) 323
FaeI CATG 3 cut(s) 172, 205, 860
FaqI GGGAC 1 cut(s) 862
FatI CATG 3 cut(s) 168, 201, 856
FauI CCCGC 1 cut(s) 547
FbaI TGATCA 2 cut(s) 405, 777
Fnu4HI GCNGC 3 cut(s) 365, 522, 612
FokI GGATG 5 cut(s) 107, 395, 698, 799, 926
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 3 cut(s) 365, 522, 612
FspBI CTAG 1 cut(s) 701
GluI GCNGC 3 cut(s) 365, 522, 612
GsaI CCCAGC 1 cut(s) 416
HaeIII GGCC 3 cut(s) 321, 635, 761
HapII CCGG 3 cut(s) 51, 138, 1010
Hin1II CATG 3 cut(s) 172, 205, 860
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 2 cut(s) 80, 491
HinfI GANTC 3 cut(s) 42, 292, 935
HpaII CCGG 3 cut(s) 51, 138, 1010
HphI GGTGA 3 cut(s) 16, 383, 941
Hpy166II GTNNAC 1 cut(s) 850
Hpy188I TCNGA 6 cut(s) 102, 480, 499, 676, 841, 1054
Hpy188III TCNNGA 5 cut(s) 71, 86, 283, 911, 988
Hpy8I GTNNAC 1 cut(s) 850
Hpy99I CGWCG 1 cut(s) 599
HpyAV CCTTC 5 cut(s) 68, 443, 731, 1049, 1055
HpyCH4III ACNGT 3 cut(s) 10, 749, 920
HpyCH4IV ACGT 1 cut(s) 904
HpyCH4V TGCA 7 cut(s) 107, 116, 464, 521, 752, 833, 953
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 308, 758
HpyF3I CTNAG 2 cut(s) 14, 673
HpySE526I ACGT 1 cut(s) 904
Hsp92II CATG 3 cut(s) 172, 205, 860
KpnI GGTACC 1 cut(s) 1068
Ksp22I TGATCA 2 cut(s) 405, 777
Kzo9I GATC 7 cut(s) 252, 405, 589, 777, 813, 924, 984
LmnI GCTCC 3 cut(s) 184, 529, 801
Lsp1109I GCAGC 2 cut(s) 351, 533
LweI GCATC 3 cut(s) 451, 676, 820
MaeI CTAG 1 cut(s) 701
MaeII ACGT 1 cut(s) 904
MaeIII GTNAC 3 cut(s) 4, 160, 371
MalI GATC 7 cut(s) 254, 407, 591, 779, 815, 926, 986
MboI GATC 7 cut(s) 252, 405, 589, 777, 813, 924, 984
MboII GAAGA 3 cut(s) 247, 1085, 1126
MfeI CAATTG 1 cut(s) 781
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 3 cut(s) 323, 719, 806
MlsI TGGCCA 1 cut(s) 635
MluCI AATT 4 cut(s) 33, 132, 781, 1024
MluNI TGGCCA 1 cut(s) 635
MlyI GAGTC 1 cut(s) 944
MmeI TCCRAC 1 cut(s) 551
Mox20I TGGCCA 1 cut(s) 635
MscI TGGCCA 1 cut(s) 635
MseI TTAA 2 cut(s) 36, 969
MslI CAYNNNNRTG 2 cut(s) 167, 809
Msp20I TGGCCA 1 cut(s) 635
MspCI CTTAAG 1 cut(s) 968
MspI CCGG 3 cut(s) 51, 138, 1010
MspR9I CCNGG 2 cut(s) 51, 569
MunI CAATTG 1 cut(s) 781
MvaI CCWGG 1 cut(s) 569
MwoI GCNNNNNNNGC 3 cut(s) 113, 308, 758
NciI CCSGG 1 cut(s) 51
NdeII GATC 7 cut(s) 252, 405, 589, 777, 813, 924, 984
NlaIII CATG 3 cut(s) 172, 205, 860
NlaIV GGNNCC 4 cut(s) 55, 310, 321, 1066
NmuCI GTSAC 3 cut(s) 4, 160, 371
NspV TTCGAA 1 cut(s) 735
PfeI GAWTC 2 cut(s) 42, 292
PkrI GCNGC 3 cut(s) 366, 523, 613
PleI GAGTC 1 cut(s) 943
PpsI GAGTC 1 cut(s) 943
Ppu21I YACGTR 1 cut(s) 905
Psp6I CCWGG 1 cut(s) 567
PspEI GGTNACC 1 cut(s) 371
PspFI CCCAGC 1 cut(s) 412
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 4 cut(s) 55, 310, 321, 1066
PspOMI GGGCCC 1 cut(s) 319
PspPI GGNCC 3 cut(s) 98, 319, 320
PsrI GAACNNNNNNTAC 2 cut(s) 565, 597
PstI CTGCAG 1 cut(s) 523
PstNI CAGNNNCTG 1 cut(s) 725
PsuI RGATCY 1 cut(s) 252
RsaI GTAC 6 cut(s) 341, 349, 514, 583, 746, 1066
RsaNI GTAC 6 cut(s) 340, 348, 513, 582, 745, 1065
RseI CAYNNNNRTG 2 cut(s) 167, 809
SaqAI TTAA 2 cut(s) 36, 969
SatI GCNGC 3 cut(s) 365, 522, 612
Sau3AI GATC 7 cut(s) 252, 405, 589, 777, 813, 924, 984
Sau96I GGNCC 3 cut(s) 98, 319, 320
SchI GAGTC 1 cut(s) 944
ScrFI CCNGG 2 cut(s) 51, 569
SduI GDGCHC 3 cut(s) 323, 719, 806
SfaNI GCATC 3 cut(s) 451, 676, 820
SfcI CTRYAG 1 cut(s) 519
SfuI TTCGAA 1 cut(s) 735
SinI GGWCC 1 cut(s) 98
SmiMI CAYNNNNRTG 2 cut(s) 167, 809
SmlI CTYRAG 2 cut(s) 383, 968
SmoI CTYRAG 2 cut(s) 383, 968
SnaBI TACGTA 1 cut(s) 905
Sse9I AATT 4 cut(s) 33, 132, 781, 1024
SsiI CCGC 3 cut(s) 302, 540, 612
SspMI CTAG 1 cut(s) 701
StyD4I CCNGG 2 cut(s) 49, 567
TaaI ACNGT 3 cut(s) 10, 749, 920
TaiI ACGT 1 cut(s) 907
TaqI TCGA 8 cut(s) 196, 290, 459, 552, 597, 735, 816, 1086
TasI AATT 4 cut(s) 33, 132, 781, 1024
TatI WGTACW 1 cut(s) 744
TauI GCSGC 1 cut(s) 614
TfiI GAWTC 2 cut(s) 42, 292
Tru1I TTAA 2 cut(s) 36, 969
Tru9I TTAA 2 cut(s) 36, 969
TscAI CASTG 5 cut(s) 15, 447, 730, 754, 1086
TseFI GTSAC 3 cut(s) 4, 160, 371
TseI GCWGC 2 cut(s) 364, 521
Tsp45I GTSAC 3 cut(s) 4, 160, 371
TspDTI ATGAA 3 cut(s) 222, 845, 1086
TspGWI ACGGA 1 cut(s) 971
TspRI CASTG 5 cut(s) 15, 447, 730, 754, 1086
Vha464I CTTAAG 1 cut(s) 968
VpaK11BI GGWCC 1 cut(s) 98
XapI RAATTY 1 cut(s) 1024
XcmI CCANNNNNNNNNTGG 1 cut(s) 1102
XspI CTAG 1 cut(s) 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.