MD02G1168000.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001289
Physical Location & Seq
Reverse (-)
14412879 .. 14415461
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1168000.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGTCCACTTGTATGACAAAGAGAAAGACAGTGACAGTTCTAAGCATCGATGGAGGTGGCATTAAAGGTCTAATTCCCAGCACCCTCCTCGCCTTTCTTGAATCCAAGCTTCAGGATTTGGATGGGCCTGATGCAAGAATTGCAGATTATTTCAACACAATTGCTGGAACAAGCACCGGTGGTCTTATCACCACCATGCTTACAGCTCCCAACAAAGACAATCGGCCCATGTACCAAGCAAAGGACATCACCACCTTCTATTTAGATCACTGCCCTAAGATTTTCCCTCAGAACAGAGGAAAAAACTTGTTGACATCAATAAAAAGTTTGGTTGGTGCTGTCATGGGGCCAAAATATGACGGCAAGTACCTGCGCACATTGTTGAGTGGTCTGCTTGGTGACCTCACTCTCAAAGACACTCTAACTAATGTGCTTATTCCATCTTTCGATATCAAGTACCTTCAGCCTGTCGTCTTCTCCACCATTGATGCAAAAGATCATCCTTTGAAAAATGCTAAGCTCTCGGATACCTGTGTAAGTACCTCTGCTGCTCCTACCTATCTCCCTGCACACTACTTCGATGTCAAGGATGATGAGGGAAGGACTCGCACTTTTGATCTCGTCGATGGAGGGGTTGCTGCAAACAATCCTACAATGATGGCCATAAGTCACATAAATAGAGAGATGTTGAGGAAAGGCTCGGATCCGATGGATGCGAAGAGGTTGTTAGTGCTATCGTTGGGCACAGGCACCGCCAAGTTCGAAGAGAAGTACAATGCAGCCACGGCCTCCAAATGGGGTTTGATCAATTGGGTCTTGCACAATGGGAACACACCTTTGGTAGACATTTTCAGCGATGCGAGTTCTGATATGGTTGATATTCATGTGTCCACCCTCTTCCAATGCATCCACGCCAAGCACAACTATCTACGTATTCAGGATGACTCATTGACCGGTGATGAAGCATCAGTAGATATAGCAACGGAGAAGAATCTTATGAGGCTTATAGAGATCGGGAATGCGTTGTTGAAGAAGCCAGTTTCACGGGTGCAGTTGGATACGGGAAGGTATGAGAAACCTGAGGTAGAAGAGGGTACCTATGAAGAAGCACTGATCAAGTTTTGCCAAAAGGTTATCAGAAGTAAAGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

385

Amino Acids

42.32

Weight (kDa)

6.46

Isoelectric Point (pI)

28.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 222 2.3e-24 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 374
Acc36I ACCTGC 1 cut(s) 378
Acc65I GGTACC 1 cut(s) 1094
AccB1I GGYRCC 2 cut(s) 749, 1094
AccI GTMKAC 1 cut(s) 843
AciI CCGC 1 cut(s) 753
AclWI GGATC 2 cut(s) 698, 711
AcoI YGGCCR 1 cut(s) 660
AcuI CTGAAG 2 cut(s) 95, 446
AfaI GTAC 6 cut(s) 233, 368, 458, 541, 773, 1096
AfiI CCNNNNNNNGG 2 cut(s) 241, 795
AgeI ACCGGT 2 cut(s) 176, 953
AgsI TTSAA 4 cut(s) 101, 154, 508, 1030
AjuI GAANNNNNNNTTGG 2 cut(s) 821, 853
AluBI AGCT 3 cut(s) 109, 206, 520
AluI AGCT 3 cut(s) 109, 206, 520
AlwI GGATC 2 cut(s) 698, 711
AoxI GGCC 5 cut(s) 125, 224, 347, 660, 786
ApeKI GCWGC 3 cut(s) 548, 638, 779
AsiGI ACCGGT 2 cut(s) 176, 953
Asp718I GGTACC 1 cut(s) 1094
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 3 cut(s) 125, 225, 347
AsuHPI GGTGA 4 cut(s) 181, 241, 410, 968
AsuII TTCGAA 1 cut(s) 762
AxyI CCTNAGG 1 cut(s) 1080
BaeGI GKGCMC 1 cut(s) 746
BaeI ACNNNNGTAYC 2 cut(s) 519, 552
BalI TGGCCA 1 cut(s) 662
BamHI GGATCC 1 cut(s) 703
BanI GGYRCC 2 cut(s) 749, 1094
BbsI GAAGAC 1 cut(s) 466
BbvI GCAGC 3 cut(s) 535, 625, 791
BccI CCATC 6 cut(s) 44, 116, 448, 620, 652, 703
BceAI ACGGC 2 cut(s) 376, 801
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciVI GTATCC 2 cut(s) 520, 1051
BclI TGATCA 2 cut(s) 804, 1113
BfuAI ACCTGC 1 cut(s) 378
BfuI GTATCC 2 cut(s) 520, 1051
BisI GCNGC 3 cut(s) 549, 639, 780
BlpI GCTNAGC 1 cut(s) 516
BlsI GCNGC 3 cut(s) 550, 640, 781
BmgT120I GGNCC 3 cut(s) 125, 225, 347
BmiI GGNNCC 4 cut(s) 348, 705, 751, 1096
BmsI GCATC 7 cut(s) 54, 121, 478, 703, 847, 915, 974
BpiI GAAGAC 1 cut(s) 466
Bpu1102I GCTNAGC 1 cut(s) 516
Bpu14I TTCGAA 1 cut(s) 762
Bsa29I ATCGAT 1 cut(s) 48
BsaAI YACGTR 1 cut(s) 932
BsaJI CCNNGG 1 cut(s) 783
BsaWI WCCGGW 2 cut(s) 176, 953
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 795
Bse118I RCCGGY 2 cut(s) 176, 953
Bse1I ACTGG 1 cut(s) 1037
Bse21I CCTNAGG 1 cut(s) 1080
BseCI ATCGAT 1 cut(s) 48
BseDI CCNNGG 1 cut(s) 783
BseGI GGATG 6 cut(s) 127, 499, 595, 718, 906, 946
BseLI CCNNNNNNNGG 2 cut(s) 241, 795
BseMII CTCAG 2 cut(s) 302, 1071
BseNI ACTGG 1 cut(s) 1037
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 746
BseXI GCAGC 3 cut(s) 535, 625, 791
BseYI CCCAGC 1 cut(s) 77
BsgI GTGCAG 2 cut(s) 552, 1070
BshFI GGCC 5 cut(s) 127, 226, 349, 662, 788
BshNI GGYRCC 2 cut(s) 749, 1094
BshTI ACCGGT 2 cut(s) 176, 953
BshVI ATCGAT 1 cut(s) 48
BsiSI CCGG 2 cut(s) 177, 954
BslI CCNNNNNNNGG 2 cut(s) 241, 795
BsmI GAATGC 1 cut(s) 1024
BsnI GGCC 5 cut(s) 127, 226, 349, 662, 788
Bsp119I TTCGAA 1 cut(s) 762
Bsp1286I GDGCHC 1 cut(s) 746
Bsp143I GATC 7 cut(s) 265, 496, 616, 703, 804, 1011, 1113
Bsp1720I GCTNAGC 1 cut(s) 516
BspACI CCGC 1 cut(s) 753
BspANI GGCC 5 cut(s) 127, 226, 349, 662, 788
BspCNI CTCAG 2 cut(s) 301, 1072
BspDI ATCGAT 1 cut(s) 48
BspLI GGNNCC 4 cut(s) 348, 705, 751, 1096
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 2 cut(s) 698, 711
BspT104I TTCGAA 1 cut(s) 762
BspT107I GGYRCC 2 cut(s) 749, 1094
BsrFI RCCGGY 2 cut(s) 176, 953
BsrI ACTGG 1 cut(s) 1037
BssAI RCCGGY 2 cut(s) 176, 953
BssECI CCNNGG 1 cut(s) 783
BssMI GATC 7 cut(s) 265, 496, 616, 703, 804, 1011, 1113
Bst4CI ACNGT 2 cut(s) 31, 37
Bst6I CTCTTC 4 cut(s) 713, 759, 902, 1083
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 932
BstBI TTCGAA 1 cut(s) 762
BstDEI CTNAG 5 cut(s) 41, 276, 288, 516, 1080
BstDSI CCRYGG 1 cut(s) 783
BstEII GGTNACC 1 cut(s) 398
BstF5I GGATG 6 cut(s) 127, 499, 595, 718, 906, 946
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 7 cut(s) 268, 499, 619, 706, 807, 1014, 1116
BstMBI GATC 7 cut(s) 265, 496, 616, 703, 804, 1011, 1113
BstMWI GCNNNNNNNGC 2 cut(s) 140, 785
BstPI GGTNACC 1 cut(s) 398
BstSLI GKGCMC 1 cut(s) 746
BstSNI TACGTA 1 cut(s) 932
BstV1I GCAGC 3 cut(s) 535, 625, 791
BstV2I GAAGAC 1 cut(s) 466
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
Bsu15I ATCGAT 1 cut(s) 48
Bsu36I CCTNAGG 1 cut(s) 1080
BsuI GTATCC 2 cut(s) 520, 1051
BsuRI GGCC 5 cut(s) 127, 226, 349, 662, 788
BsuTUI ATCGAT 1 cut(s) 48
BtgI CCRYGG 1 cut(s) 783
BtgZI GCGATG 1 cut(s) 870
BtsCI GGATG 6 cut(s) 127, 499, 595, 718, 906, 946
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 3 cut(s) 36, 268, 1109
BveI ACCTGC 1 cut(s) 378
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 2 cut(s) 176, 953
Cfr13I GGNCC 3 cut(s) 125, 225, 347
ClaI ATCGAT 1 cut(s) 48
Csp6I GTAC 6 cut(s) 232, 367, 457, 540, 772, 1095
CspAI ACCGGT 2 cut(s) 176, 953
CviAII CATG 4 cut(s) 196, 229, 343, 884
CviQI GTAC 6 cut(s) 232, 367, 457, 540, 772, 1095
DdeI CTNAG 5 cut(s) 41, 276, 288, 516, 1080
DpnI GATC 7 cut(s) 267, 498, 618, 705, 806, 1013, 1115
DpnII GATC 7 cut(s) 265, 496, 616, 703, 804, 1011, 1113
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 4 cut(s) 713, 759, 902, 1083
EarI CTCTTC 4 cut(s) 713, 759, 902, 1083
Eco105I TACGTA 1 cut(s) 932
Eco32I GATATC 1 cut(s) 451
Eco57I CTGAAG 2 cut(s) 95, 446
Eco81I CCTNAGG 1 cut(s) 1080
Eco91I GGTNACC 1 cut(s) 398
EcoO65I GGTNACC 1 cut(s) 398
EcoRV GATATC 1 cut(s) 451
EcoT22I ATGCAT 1 cut(s) 908
FaeI CATG 4 cut(s) 199, 232, 346, 887
FatI CATG 4 cut(s) 195, 228, 342, 883
FbaI TGATCA 2 cut(s) 804, 1113
FblI GTMKAC 1 cut(s) 843
Fnu4HI GCNGC 3 cut(s) 549, 639, 780
FokI GGATG 6 cut(s) 134, 486, 602, 725, 893, 953
Fsp4HI GCNGC 3 cut(s) 549, 639, 780
FspI TGCGCA 1 cut(s) 374
GlaI GCGC 1 cut(s) 374
GluI GCNGC 3 cut(s) 549, 639, 780
GsaI CCCAGC 1 cut(s) 81
HaeIII GGCC 5 cut(s) 127, 226, 349, 662, 788
HapII CCGG 2 cut(s) 177, 954
HhaI GCGC 1 cut(s) 375
Hin1II CATG 4 cut(s) 199, 232, 346, 887
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 4 cut(s) 101, 604, 944, 991
HpaII CCGG 2 cut(s) 177, 954
HphI GGTGA 4 cut(s) 181, 241, 410, 968
Hpy166II GTNNAC 4 cut(s) 6, 312, 844, 891
Hpy188I TCNGA 6 cut(s) 291, 526, 703, 708, 868, 1139
Hpy188III TCNNGA 4 cut(s) 98, 113, 938, 1015
Hpy8I GTNNAC 4 cut(s) 6, 312, 844, 891
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 4 cut(s) 265, 470, 594, 1059
HpyCH4III ACNGT 2 cut(s) 31, 37
HpyCH4IV ACGT 1 cut(s) 931
HpyCH4V TGCA 9 cut(s) 134, 143, 491, 569, 641, 779, 820, 906, 1051
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 785
HpyF3I CTNAG 5 cut(s) 41, 276, 288, 516, 1080
HpySE526I ACGT 1 cut(s) 931
Hsp92II CATG 4 cut(s) 199, 232, 346, 887
HspAI GCGC 1 cut(s) 373
KpnI GGTACC 1 cut(s) 1098
Ksp22I TGATCA 2 cut(s) 804, 1113
Kzo9I GATC 7 cut(s) 265, 496, 616, 703, 804, 1011, 1113
LmnI GCTCC 2 cut(s) 211, 556
Lsp1109I GCAGC 3 cut(s) 535, 625, 791
LweI GCATC 7 cut(s) 54, 121, 478, 703, 847, 915, 974
MaeII ACGT 1 cut(s) 931
MaeIII GTNAC 3 cut(s) 31, 398, 668
MalI GATC 7 cut(s) 267, 498, 618, 705, 806, 1013, 1115
MboI GATC 7 cut(s) 265, 496, 616, 703, 804, 1011, 1113
MboII GAAGA 8 cut(s) 466, 730, 776, 889, 1000, 1042, 1100, 1115
MfeI CAATTG 2 cut(s) 159, 808
MflI RGATCY 1 cut(s) 703
MhlI GDGCHC 1 cut(s) 746
MlsI TGGCCA 1 cut(s) 662
MluCI AATT 4 cut(s) 72, 138, 159, 808
MluNI TGGCCA 1 cut(s) 662
MlyI GAGTC 2 cut(s) 598, 938
MmeI TCCRAC 1 cut(s) 1035
Mox20I TGGCCA 1 cut(s) 662
Mph1103I ATGCAT 1 cut(s) 908
MscI TGGCCA 1 cut(s) 662
MseI TTAA 1 cut(s) 63
MslI CAYNNNNRTG 2 cut(s) 11, 194
Msp20I TGGCCA 1 cut(s) 662
MspI CCGG 2 cut(s) 177, 954
MunI CAATTG 2 cut(s) 159, 808
Mva1269I GAATGC 1 cut(s) 1024
MwoI GCNNNNNNNGC 2 cut(s) 140, 785
NdeII GATC 7 cut(s) 265, 496, 616, 703, 804, 1011, 1113
NlaIII CATG 4 cut(s) 199, 232, 346, 887
NlaIV GGNNCC 4 cut(s) 348, 705, 751, 1096
NmuCI GTSAC 3 cut(s) 31, 398, 668
NsbI TGCGCA 1 cut(s) 374
NsiI ATGCAT 1 cut(s) 908
NspV TTCGAA 1 cut(s) 762
PctI GAATGC 1 cut(s) 1024
PfeI GAWTC 2 cut(s) 101, 991
PinAI ACCGGT 2 cut(s) 176, 953
PkrI GCNGC 3 cut(s) 550, 640, 781
PleI GAGTC 2 cut(s) 598, 938
PpsI GAGTC 2 cut(s) 598, 938
Ppu21I YACGTR 1 cut(s) 932
PspEI GGTNACC 1 cut(s) 398
PspFI CCCAGC 1 cut(s) 77
PspN4I GGNNCC 4 cut(s) 348, 705, 751, 1096
PspPI GGNCC 3 cut(s) 125, 225, 347
PsuI RGATCY 1 cut(s) 703
RsaI GTAC 6 cut(s) 233, 368, 458, 541, 773, 1096
RsaNI GTAC 6 cut(s) 232, 367, 457, 540, 772, 1095
RseI CAYNNNNRTG 2 cut(s) 11, 194
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 3 cut(s) 549, 639, 780
Sau3AI GATC 7 cut(s) 265, 496, 616, 703, 804, 1011, 1113
Sau96I GGNCC 3 cut(s) 125, 225, 347
SchI GAGTC 2 cut(s) 598, 938
SduI GDGCHC 1 cut(s) 746
SfaNI GCATC 7 cut(s) 54, 121, 478, 703, 847, 915, 974
SfuI TTCGAA 1 cut(s) 762
SgrAI CRCCGGYG 1 cut(s) 176
SmiMI CAYNNNNRTG 2 cut(s) 11, 194
SnaBI TACGTA 1 cut(s) 932
Sse9I AATT 4 cut(s) 72, 138, 159, 808
SsiI CCGC 1 cut(s) 753
TaaI ACNGT 2 cut(s) 31, 37
TaiI ACGT 1 cut(s) 934
TaqI TCGA 5 cut(s) 48, 447, 579, 624, 762
TasI AATT 4 cut(s) 72, 138, 159, 808
TatI WGTACW 1 cut(s) 771
TfiI GAWTC 2 cut(s) 101, 991
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 3 cut(s) 36, 275, 1116
TseFI GTSAC 3 cut(s) 31, 398, 668
TseI GCWGC 3 cut(s) 548, 638, 779
Tsp45I GTSAC 3 cut(s) 31, 398, 668
TspDTI ATGAA 3 cut(s) 872, 975, 1116
TspGWI ACGGA 1 cut(s) 998
TspRI CASTG 3 cut(s) 36, 275, 1116
XmiI GTMKAC 1 cut(s) 843
Zsp2I ATGCAT 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.