RchiOBHm_Chr2g0104901

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001289
Physical Location & Seq
Reverse (-)
16165551 .. 16166968
1418 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47914

Sequence Viewer

Length: 828 bp
ATGAAGCGGGGGCCAAAATACGATGGGATGTATCTGCGCTCATTGATAAACGGGCTCCTTGGTGATCTCACTCTCAAGCAGACTTTAACCAATGTGGTCATTCCAAGTTTTGATATCAAACACCTTCAGCCTGTGGTTTTCTCAACCATCGATGCAAAAGAAAATTCATTGAAGAACGCAAAGCTTTCAGATGTCTGCATCAGTACCTCTGCAGCTCCATATTATCTCCCTCCACACTACTTCGAAGTCAAGGATGACCAGGGAAATATCCGCAAATTTGATCTTGTCGATGGTGGGGTTGCTGCAAATAATCCTACAATGTTGGCCATAAGCCACATAAACAGAGAGATGTTGAAGAACAACTCAGAACCAATAAATGCGAATCGCTTGCAAGTGCTGTCATTGGGCACTGGCGCTGCCAAGTTTGAGGAGAAGTACAGTGCAGTCGAGGCATCAAAATGGGGTTTGGTGAATTGGATGTTTAATAATGGGAGCACACCTTTGCTGGACATTTTTGGGGATGCAAGTTCTGATATGGTTGGCTTTCATGTCTCCACTCTCTTCCAGTCCCTCCATCTCAAGGACAATTACCTACGAATTCAGGATGACACACTGAGCGGAGATGAATCATCAGTGGATATTGCAACCGAGAAGAATCTGAAAAGCCTTGTGGAGATCGGAAATGTGTTGCTGAAGAAGCCAGTGTCAAGGGTAAATCTCGATACTGGGAGGTACGAGGAATCTGAGGGAGACGGTACCTACGAAGAAGCACTGGTCGATTTTGCCAAAGGGCTCGCGGAGGGAAAGAAGCTCGGGCAAAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

275

Amino Acids

30.31

Weight (kDa)

5.46

Isoelectric Point (pI)

31.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 3 - 110 2.2e-13 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 755
AccB1I GGYRCC 1 cut(s) 755
AccBSI CCGCTC 1 cut(s) 618
AccII CGCG 1 cut(s) 797
AciI CCGC 4 cut(s) 7, 271, 618, 797
AcoI YGGCCR 1 cut(s) 324
AcsI RAATTY 3 cut(s) 163, 275, 597
AcuI CTGAAG 2 cut(s) 110, 713
AfaI GTAC 4 cut(s) 205, 437, 734, 757
AfiI CCNNNNNNNGG 1 cut(s) 580
AgsI TTSAA 2 cut(s) 172, 355
AjnI CCWGG 1 cut(s) 258
AluBI AGCT 3 cut(s) 184, 215, 811
AluI AGCT 3 cut(s) 184, 215, 811
Alw21I GWGCWC 1 cut(s) 497
Alw26I GTCTC 2 cut(s) 556, 744
Ama87I CYCGRG 1 cut(s) 812
AoxI GGCC 2 cut(s) 11, 324
ApeKI GCWGC 3 cut(s) 212, 302, 416
ApoI RAATTY 3 cut(s) 163, 275, 597
Asp718I GGTACC 1 cut(s) 755
AspLEI GCGC 2 cut(s) 39, 416
AspS9I GGNCC 1 cut(s) 11
AsuHPI GGTGA 2 cut(s) 74, 481
AsuII TTCGAA 1 cut(s) 243
AvaI CYCGRG 1 cut(s) 812
BaeGI GKGCMC 1 cut(s) 410
BalI TGGCCA 1 cut(s) 326
BanI GGYRCC 1 cut(s) 755
BanII GRGCYC 2 cut(s) 57, 795
Bbv12I GWGCWC 1 cut(s) 497
BbvI GCAGC 3 cut(s) 224, 289, 403
BccI CCATC 4 cut(s) 17, 155, 284, 582
BcgI CGANNNNNNTGC 2 cut(s) 370, 404
BciT130I CCWGG 1 cut(s) 260
BcoDI GTCTC 2 cut(s) 556, 744
BfmI CTRYAG 1 cut(s) 210
BfoI RGCGCY 1 cut(s) 417
BisI GCNGC 3 cut(s) 213, 303, 417
BlsI GCNGC 3 cut(s) 214, 304, 418
Bme1390I CCNGG 1 cut(s) 260
BmeT110I CYCGRG 1 cut(s) 812
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 3 cut(s) 12, 56, 757
BmrFI CCNGG 1 cut(s) 260
BmrI ACTGGG 1 cut(s) 735
BmsI GCATC 4 cut(s) 142, 207, 461, 511
BmuI ACTGGG 1 cut(s) 735
Bpu14I TTCGAA 1 cut(s) 243
BpuEI CTTGAG 2 cut(s) 59, 563
Bsa29I ATCGAT 1 cut(s) 150
BsaJI CCNNGG 2 cut(s) 58, 259
Bsc4I CCNNNNNNNGG 1 cut(s) 580
Bse1I ACTGG 5 cut(s) 415, 565, 701, 730, 777
BseBI CCWGG 1 cut(s) 260
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 2 cut(s) 58, 259
BseGI GGATG 5 cut(s) 33, 259, 483, 526, 610
BseLI CCNNNNNNNGG 1 cut(s) 580
BseMII CTCAG 3 cut(s) 378, 605, 735
BseNI ACTGG 5 cut(s) 415, 565, 701, 730, 777
BseRI GAGGAG 1 cut(s) 443
BseSI GKGCMC 1 cut(s) 410
BseXI GCAGC 3 cut(s) 224, 289, 403
BsgI GTGCAG 1 cut(s) 462
Bsh1236I CGCG 1 cut(s) 797
BshFI GGCC 2 cut(s) 13, 326
BshNI GGYRCC 1 cut(s) 755
BshVI ATCGAT 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 497
BsiHKCI CYCGRG 1 cut(s) 812
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 1 cut(s) 580
BsmAI GTCTC 2 cut(s) 556, 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 553
BsnI GGCC 2 cut(s) 13, 326
BsoBI CYCGRG 1 cut(s) 812
Bsp119I TTCGAA 1 cut(s) 243
Bsp1286I GDGCHC 4 cut(s) 57, 410, 497, 795
Bsp143I GATC 3 cut(s) 64, 280, 675
BspACI CCGC 4 cut(s) 7, 271, 618, 797
BspANI GGCC 2 cut(s) 13, 326
BspCNI CTCAG 3 cut(s) 377, 606, 736
BspDI ATCGAT 1 cut(s) 150
BspFNI CGCG 1 cut(s) 797
BspLI GGNNCC 3 cut(s) 12, 56, 757
BspMAI CTGCAG 1 cut(s) 214
BspT104I TTCGAA 1 cut(s) 243
BspT107I GGYRCC 1 cut(s) 755
BsrBI CCGCTC 1 cut(s) 618
BsrI ACTGG 5 cut(s) 415, 565, 701, 730, 777
BssECI CCNNGG 2 cut(s) 58, 259
BssMI GATC 3 cut(s) 64, 280, 675
BssT1I CCWWGG 1 cut(s) 58
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 2 cut(s) 440, 755
Bst6I CTCTTC 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 243
BstC8I GCNNGC 2 cut(s) 389, 795
BstDEI CTNAG 3 cut(s) 364, 614, 744
BstF5I GGATG 5 cut(s) 33, 259, 483, 526, 610
BstFNI CGCG 1 cut(s) 797
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 2 cut(s) 39, 416
BstKTI GATC 3 cut(s) 67, 283, 678
BstMAI GTCTC 2 cut(s) 556, 744
BstMBI GATC 3 cut(s) 64, 280, 675
BstMWI GCNNNNNNNGC 2 cut(s) 449, 697
BstNI CCWGG 1 cut(s) 260
BstSCI CCNGG 1 cut(s) 258
BstSFI CTRYAG 1 cut(s) 210
BstSLI GKGCMC 1 cut(s) 410
BstUI CGCG 1 cut(s) 797
BstV1I GCAGC 3 cut(s) 224, 289, 403
Bsu15I ATCGAT 1 cut(s) 150
BsuRI GGCC 2 cut(s) 13, 326
BsuTUI ATCGAT 1 cut(s) 150
BtsCI GGATG 5 cut(s) 33, 259, 483, 526, 610
BtsIMutI CAGTG 6 cut(s) 408, 445, 611, 639, 708, 770
Cac8I GCNNGC 2 cut(s) 389, 795
CfoI GCGC 2 cut(s) 39, 416
Cfr13I GGNCC 1 cut(s) 11
ClaI ATCGAT 1 cut(s) 150
Csp6I GTAC 4 cut(s) 204, 436, 733, 756
CviAII CATG 1 cut(s) 548
CviQI GTAC 4 cut(s) 204, 436, 733, 756
DdeI CTNAG 3 cut(s) 364, 614, 744
DpnI GATC 3 cut(s) 66, 282, 677
DpnII GATC 3 cut(s) 64, 280, 675
EaeI YGGCCR 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 566
EarI CTCTTC 1 cut(s) 566
Eco130I CCWWGG 1 cut(s) 58
Eco24I GRGCYC 2 cut(s) 57, 795
Eco32I GATATC 1 cut(s) 115
Eco57I CTGAAG 2 cut(s) 110, 713
Eco88I CYCGRG 1 cut(s) 812
EcoRI GAATTC 1 cut(s) 597
EcoRII CCWGG 1 cut(s) 258
EcoRV GATATC 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 58
EcoT38I GRGCYC 2 cut(s) 57, 795
ErhI CCWWGG 1 cut(s) 58
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 1 cut(s) 551
FaiI YATR 5 cut(s) 220, 329, 338, 536, 549
FaqI GGGAC 1 cut(s) 553
FatI CATG 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 213, 303, 417
FokI GGATG 5 cut(s) 40, 266, 490, 533, 617
FriOI GRGCYC 2 cut(s) 57, 795
Fsp4HI GCNGC 3 cut(s) 213, 303, 417
GlaI GCGC 2 cut(s) 38, 415
GluI GCNGC 3 cut(s) 213, 303, 417
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 2 cut(s) 13, 326
HhaI GCGC 2 cut(s) 39, 416
Hin1II CATG 1 cut(s) 551
Hin6I GCGC 2 cut(s) 37, 414
HinP1I GCGC 2 cut(s) 37, 414
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 4 cut(s) 382, 626, 655, 740
HphI GGTGA 2 cut(s) 74, 481
Hpy188I TCNGA 6 cut(s) 190, 367, 532, 660, 680, 745
Hpy188III TCNNGA 2 cut(s) 602, 719
HpyAV CCTTC 1 cut(s) 134
HpyCH4III ACNGT 2 cut(s) 440, 755
HpyCH4V TGCA 8 cut(s) 155, 198, 212, 305, 391, 443, 524, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 697
HpyF3I CTNAG 3 cut(s) 364, 614, 744
Hsp92II CATG 1 cut(s) 551
HspAI GCGC 2 cut(s) 37, 414
KpnI GGTACC 1 cut(s) 759
Kzo9I GATC 3 cut(s) 64, 280, 675
LmnI GCTCC 3 cut(s) 60, 220, 492
Lsp1109I GCAGC 3 cut(s) 224, 289, 403
LweI GCATC 4 cut(s) 142, 207, 461, 511
MalI GATC 3 cut(s) 66, 282, 677
MbiI CCGCTC 1 cut(s) 618
MboI GATC 3 cut(s) 64, 280, 675
MboII GAAGA 6 cut(s) 184, 367, 553, 664, 706, 776
MhlI GDGCHC 4 cut(s) 57, 410, 497, 795
MlsI TGGCCA 1 cut(s) 326
MluCI AATT 6 cut(s) 163, 275, 472, 586, 597, 823
MluNI TGGCCA 1 cut(s) 326
MnlI CCTC 9 cut(s) 217, 240, 421, 442, 581, 723, 730, 739, 793
Mox20I TGGCCA 1 cut(s) 326
MscI TGGCCA 1 cut(s) 326
MseI TTAA 2 cut(s) 86, 483
MslI CAYNNNNRTG 1 cut(s) 457
Msp20I TGGCCA 1 cut(s) 326
MspR9I CCNGG 1 cut(s) 260
MvaI CCWGG 1 cut(s) 260
MvnI CGCG 1 cut(s) 797
MwoI GCNNNNNNNGC 2 cut(s) 449, 697
NdeII GATC 3 cut(s) 64, 280, 675
NlaIII CATG 1 cut(s) 551
NlaIV GGNNCC 3 cut(s) 12, 56, 757
NspV TTCGAA 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PfeI GAWTC 4 cut(s) 382, 626, 655, 740
PkrI GCNGC 3 cut(s) 214, 304, 418
Psp6I CCWGG 1 cut(s) 258
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 3 cut(s) 12, 56, 757
PspPI GGNCC 1 cut(s) 11
PstI CTGCAG 1 cut(s) 214
RsaI GTAC 4 cut(s) 205, 437, 734, 757
RsaNI GTAC 4 cut(s) 204, 436, 733, 756
RseI CAYNNNNRTG 1 cut(s) 457
SaqAI TTAA 2 cut(s) 86, 483
SatI GCNGC 3 cut(s) 213, 303, 417
Sau3AI GATC 3 cut(s) 64, 280, 675
Sau96I GGNCC 1 cut(s) 11
ScrFI CCNGG 1 cut(s) 260
SduI GDGCHC 4 cut(s) 57, 410, 497, 795
SetI ASST 9 cut(s) 126, 186, 209, 217, 502, 594, 734, 761, 813
SfaNI GCATC 4 cut(s) 142, 207, 461, 511
SfcI CTRYAG 1 cut(s) 210
SfuI TTCGAA 1 cut(s) 243
SmiMI CAYNNNNRTG 1 cut(s) 457
SmlI CTYRAG 2 cut(s) 74, 578
SmoI CTYRAG 2 cut(s) 74, 578
Sse9I AATT 6 cut(s) 163, 275, 472, 586, 597, 823
SsiI CCGC 4 cut(s) 7, 271, 618, 797
StyD4I CCNGG 1 cut(s) 258
StyI CCWWGG 1 cut(s) 58
TaaI ACNGT 2 cut(s) 440, 755
TaqI TCGA 6 cut(s) 150, 243, 288, 447, 720, 777
TasI AATT 6 cut(s) 163, 275, 472, 586, 597, 823
TatI WGTACW 1 cut(s) 435
TfiI GAWTC 4 cut(s) 382, 626, 655, 740
Tru1I TTAA 2 cut(s) 86, 483
Tru9I TTAA 2 cut(s) 86, 483
TscAI CASTG 6 cut(s) 415, 445, 618, 639, 708, 777
TseI GCWGC 3 cut(s) 212, 302, 416
TspDTI ATGAA 4 cut(s) 17, 156, 536, 639
TspRI CASTG 6 cut(s) 415, 445, 618, 639, 708, 777
XapI RAATTY 3 cut(s) 163, 275, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.