Rw2G013720

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001289
Physical Location & Seq
Reverse (-)
14847001 .. 14850285
3285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013720.1

Sequence Viewer

Length: 1164 bp
ATGCCGACTAGCTTGGAAAAAAGAAAGATGGTGACAGTGCTAAGCATTGATGGAGGTGGAATTAAAGGAATCATACCCGGCACCCTTCTTGCCTTTCTTGAAACCAAGCTTCAGGATTTGGATGGTCCGAGTGCAAGACTTGCAGATTATTTTGATATAATTGCCGGAACAAGCGCAGGGGGGTTGGTCACTACCATGCTTACAGCTCCCAACAAGGACAATCGACCCATGTATGAAGCAAAGGACATCAACAACTTCTACTTTGAACAAACACCCAAGATCTTCCCTCAAAATAGTCAAAAGAACTTCTTGACATCGATTCAAAGTGCGGTTGGTGCCGTGATGGGCCTAAAATACGATGGGAAGTACCTGCGTACAATGACGAACAAGCTGCTCGGTGACCTTACTCTCAAGCAAACTCTAACCAATGTGATCATCCCAGCCTTTGATATCAAACACCTTCAGCCAGTGATTTTCTCAACCATCGATGCAAAAGAAAGTTCTCTGAAAAATCCAAAGCTTTCGGATATCTGTATCAGTACCTCTGCAGCTCCAACTTTTCTCCCCGCACACTACTTCGAGGTCAAGGACAGCCAGGGAACAACTCGTACTTTTGATCTCGTCGATGGTGGGGTTGCCGCAAACAACCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATGTTGAAGCACGGCTCAGAACCAATGGATGCTAATCGTTTGCTAGTGCTATCATTGGGCACAGGCACTGCCAAGTTCGAAGGGAAGTACAGTGCAACCACGGCCTCAAAATGGGGTTTGATCAATTGGATGTTTGACAATGGGAGCACACCTATGATCGATATCTTTACCGATGCAAGTTCCGATATGGTTGACATTCATGTCTCCACTCTCTTTCAGTCCCTCAATGCCAAAGACAACTACCTACGTATTCAGGATGACAGTTTGATCGGTGAGGAGTCATCGGTGGATATTGCAACGGAGAAAAATCTTAAGAGGCTTGTGGAGATCGGGAATGGGCTGTTGAGAAAGCCGGTGTCAAGGGCAAATTTGGATAACGGGAGGTATGAGAAATCTGAAGGAGAAGGTACCTATGAAGAAGCACTGGTCGATTTTGCCAAACGGCTCGTGGAGGGGAAGAAGCATAGGCAATACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

387

Amino Acids

42.61

Weight (kDa)

6.34

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 222 9.8e-25 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 884
Acc36I ACCTGC 1 cut(s) 378
Acc65I GGTACC 1 cut(s) 1091
AccB1I GGYRCC 3 cut(s) 80, 335, 1091
AciI CCGC 3 cut(s) 329, 567, 639
AcoI YGGCCR 1 cut(s) 660
AcsI RAATTY 1 cut(s) 1051
AcuI CTGAAG 3 cut(s) 95, 446, 1101
AfaI GTAC 6 cut(s) 368, 376, 541, 610, 773, 1093
AfiI CCNNNNNNNGG 1 cut(s) 795
AflII CTTAAG 1 cut(s) 995
AgsI TTSAA 4 cut(s) 101, 266, 323, 691
AjnI CCWGG 1 cut(s) 594
AluBI AGCT 6 cut(s) 12, 109, 206, 391, 520, 551
AluI AGCT 6 cut(s) 12, 109, 206, 391, 520, 551
Alw21I GWGCWC 1 cut(s) 833
Alw26I GTCTC 1 cut(s) 892
AlwNI CAGNNNCTG 1 cut(s) 752
AoxI GGCC 3 cut(s) 346, 660, 786
ApeKI GCWGC 2 cut(s) 391, 548
ApoI RAATTY 1 cut(s) 1051
Asp718I GGTACC 1 cut(s) 1091
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 125, 346
AsuC2I CCSGG 1 cut(s) 78
AsuHPI GGTGA 3 cut(s) 43, 410, 968
AsuII TTCGAA 1 cut(s) 762
AvaII GGWCC 1 cut(s) 125
BaeGI GKGCMC 1 cut(s) 746
BalI TGGCCA 1 cut(s) 662
BanI GGYRCC 3 cut(s) 80, 335, 1091
BauI CACGAG 1 cut(s) 1130
Bbv12I GWGCWC 1 cut(s) 833
BbvI GCAGC 2 cut(s) 378, 560
BccI CCATC 8 cut(s) 22, 44, 116, 337, 353, 491, 620, 652
BceAI ACGGC 4 cut(s) 323, 712, 801, 1142
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BciT130I CCWGG 1 cut(s) 596
BclI TGATCA 2 cut(s) 432, 804
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 1 cut(s) 892
BfaI CTAG 2 cut(s) 9, 728
BfmI CTRYAG 1 cut(s) 546
BfrI CTTAAG 1 cut(s) 995
BfuAI ACCTGC 1 cut(s) 378
BglII AGATCT 1 cut(s) 279
BisI GCNGC 3 cut(s) 392, 549, 639
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 3 cut(s) 393, 550, 640
Bme1390I CCNGG 2 cut(s) 78, 596
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 2 cut(s) 125, 346
BmiI GGNNCC 3 cut(s) 82, 337, 1093
BmrFI CCNGG 2 cut(s) 78, 596
BmsI GCATC 3 cut(s) 478, 703, 847
Bpu1102I GCTNAGC 1 cut(s) 41
Bpu14I TTCGAA 1 cut(s) 762
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 78
Bsa29I ATCGAT 3 cut(s) 317, 486, 843
BsaAI YACGTR 1 cut(s) 932
BsaBI GATNNNNATC 2 cut(s) 717, 845
BsaJI CCNNGG 2 cut(s) 595, 783
Bsc4I CCNNNNNNNGG 1 cut(s) 795
Bse118I RCCGGY 1 cut(s) 1036
Bse1I ACTGG 2 cut(s) 467, 1113
Bse8I GATNNNNATC 2 cut(s) 717, 845
BseBI CCWGG 1 cut(s) 596
BseCI ATCGAT 3 cut(s) 317, 486, 843
BseDI CCNNGG 2 cut(s) 595, 783
BseGI GGATG 5 cut(s) 127, 435, 718, 819, 946
BseJI GATNNNNATC 2 cut(s) 717, 845
BseLI CCNNNNNNNGG 1 cut(s) 795
BseMII CTCAG 1 cut(s) 714
BseNI ACTGG 2 cut(s) 467, 1113
BseRI GAGGAG 1 cut(s) 974
BseSI GKGCMC 1 cut(s) 746
BseXI GCAGC 2 cut(s) 378, 560
BseYI CCCAGC 1 cut(s) 439
BshFI GGCC 3 cut(s) 348, 662, 788
BshNI GGYRCC 3 cut(s) 80, 335, 1091
BshVI ATCGAT 3 cut(s) 317, 486, 843
BsiHKAI GWGCWC 1 cut(s) 833
BsiSI CCGG 3 cut(s) 78, 165, 1037
BslFI GGGAC 1 cut(s) 889
BslI CCNNNNNNNGG 1 cut(s) 795
BsmAI GTCTC 1 cut(s) 892
BsmFI GGGAC 1 cut(s) 889
BsnI GGCC 3 cut(s) 348, 662, 788
Bsp119I TTCGAA 1 cut(s) 762
Bsp1286I GDGCHC 2 cut(s) 746, 833
Bsp143I GATC 7 cut(s) 279, 432, 616, 804, 840, 951, 1011
Bsp1720I GCTNAGC 1 cut(s) 41
BspACI CCGC 3 cut(s) 329, 567, 639
BspANI GGCC 3 cut(s) 348, 662, 788
BspCNI CTCAG 1 cut(s) 713
BspDI ATCGAT 3 cut(s) 317, 486, 843
BspLI GGNNCC 3 cut(s) 82, 337, 1093
BspMAI CTGCAG 1 cut(s) 550
BspMI ACCTGC 1 cut(s) 378
BspT104I TTCGAA 1 cut(s) 762
BspT107I GGYRCC 3 cut(s) 80, 335, 1091
BspTI CTTAAG 1 cut(s) 995
BsrFI RCCGGY 1 cut(s) 1036
BsrI ACTGG 2 cut(s) 467, 1113
BssAI RCCGGY 1 cut(s) 1036
BssECI CCNNGG 2 cut(s) 595, 783
BssMI GATC 7 cut(s) 279, 432, 616, 804, 840, 951, 1011
BssSI CACGAG 1 cut(s) 1130
Bst2BI CACGAG 1 cut(s) 1130
Bst2UI CCWGG 1 cut(s) 596
Bst4CI ACNGT 3 cut(s) 37, 776, 947
BstAFI CTTAAG 1 cut(s) 995
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 932
BstBI TTCGAA 1 cut(s) 762
BstDEI CTNAG 2 cut(s) 41, 700
BstDSI CCRYGG 1 cut(s) 783
BstEII GGTNACC 1 cut(s) 398
BstF5I GGATG 5 cut(s) 127, 435, 718, 819, 946
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 7 cut(s) 282, 435, 619, 807, 843, 954, 1014
BstMAI GTCTC 1 cut(s) 892
BstMBI GATC 7 cut(s) 279, 432, 616, 804, 840, 951, 1011
BstMWI GCNNNNNNNGC 3 cut(s) 140, 335, 785
BstNI CCWGG 1 cut(s) 596
BstPI GGTNACC 1 cut(s) 398
BstSCI CCNGG 2 cut(s) 76, 594
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 746
BstSNI TACGTA 1 cut(s) 932
BstV1I GCAGC 2 cut(s) 378, 560
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
Bsu15I ATCGAT 3 cut(s) 317, 486, 843
BsuRI GGCC 3 cut(s) 348, 662, 788
BsuTUI ATCGAT 3 cut(s) 317, 486, 843
BtgI CCRYGG 1 cut(s) 783
BtsCI GGATG 5 cut(s) 127, 435, 718, 819, 946
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 5 cut(s) 42, 474, 750, 781, 1106
BveI ACCTGC 1 cut(s) 378
CaiI CAGNNNCTG 1 cut(s) 752
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 1 cut(s) 1036
Cfr13I GGNCC 2 cut(s) 125, 346
ClaI ATCGAT 3 cut(s) 317, 486, 843
Csp6I GTAC 6 cut(s) 367, 375, 540, 609, 772, 1092
CviAII CATG 3 cut(s) 196, 229, 884
CviQI GTAC 6 cut(s) 367, 375, 540, 609, 772, 1092
DdeI CTNAG 2 cut(s) 41, 700
DpnI GATC 7 cut(s) 281, 434, 618, 806, 842, 953, 1013
DpnII GATC 7 cut(s) 279, 432, 616, 804, 840, 951, 1011
DrdI GACNNNNNNGTC 1 cut(s) 884
DseDI GACNNNNNNGTC 1 cut(s) 884
EaeI YGGCCR 1 cut(s) 660
Eco105I TACGTA 1 cut(s) 932
Eco32I GATATC 3 cut(s) 451, 529, 847
Eco47I GGWCC 1 cut(s) 125
Eco57I CTGAAG 3 cut(s) 95, 446, 1101
Eco91I GGTNACC 1 cut(s) 398
EcoO65I GGTNACC 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 594
EcoRV GATATC 3 cut(s) 451, 529, 847
FaeI CATG 3 cut(s) 199, 232, 887
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 1 cut(s) 889
FatI CATG 3 cut(s) 195, 228, 883
FauI CCCGC 1 cut(s) 574
FbaI TGATCA 2 cut(s) 432, 804
Fnu4HI GCNGC 3 cut(s) 392, 549, 639
FokI GGATG 5 cut(s) 134, 422, 725, 826, 953
Fsp4HI GCNGC 3 cut(s) 392, 549, 639
FspBI CTAG 2 cut(s) 9, 728
GlaI GCGC 1 cut(s) 175
GluI GCNGC 3 cut(s) 392, 549, 639
GsaI CCCAGC 1 cut(s) 443
HaeIII GGCC 3 cut(s) 348, 662, 788
HapII CCGG 3 cut(s) 78, 165, 1037
HhaI GCGC 1 cut(s) 176
Hin1II CATG 3 cut(s) 199, 232, 887
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HindIII AAGCTT 2 cut(s) 107, 518
HinfI GANTC 3 cut(s) 69, 319, 962
HpaII CCGG 3 cut(s) 78, 165, 1037
HphI GGTGA 3 cut(s) 43, 410, 968
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 6 cut(s) 129, 507, 526, 703, 868, 1081
Hpy188III TCNNGA 5 cut(s) 98, 113, 310, 938, 1015
Hpy8I GTNNAC 1 cut(s) 877
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 5 cut(s) 95, 470, 758, 1076, 1082
HpyCH4III ACNGT 3 cut(s) 37, 776, 947
HpyCH4IV ACGT 1 cut(s) 931
HpyCH4V TGCA 7 cut(s) 134, 143, 491, 548, 779, 860, 980
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 335, 785
HpyF3I CTNAG 2 cut(s) 41, 700
HpySE526I ACGT 1 cut(s) 931
Hsp92II CATG 3 cut(s) 199, 232, 887
HspAI GCGC 1 cut(s) 174
KpnI GGTACC 1 cut(s) 1095
Ksp22I TGATCA 2 cut(s) 432, 804
Kzo9I GATC 7 cut(s) 279, 432, 616, 804, 840, 951, 1011
LmnI GCTCC 3 cut(s) 211, 556, 828
Lsp1109I GCAGC 2 cut(s) 378, 560
LweI GCATC 3 cut(s) 478, 703, 847
MaeI CTAG 2 cut(s) 9, 728
MaeII ACGT 1 cut(s) 931
MaeIII GTNAC 3 cut(s) 31, 187, 398
MalI GATC 7 cut(s) 281, 434, 618, 806, 842, 953, 1013
MboI GATC 7 cut(s) 279, 432, 616, 804, 840, 951, 1011
MboII GAAGA 3 cut(s) 274, 1112, 1153
MfeI CAATTG 1 cut(s) 808
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 2 cut(s) 746, 833
MlsI TGGCCA 1 cut(s) 662
MluCI AATT 4 cut(s) 60, 159, 808, 1051
MluNI TGGCCA 1 cut(s) 662
MlyI GAGTC 1 cut(s) 971
MmeI TCCRAC 1 cut(s) 578
Mox20I TGGCCA 1 cut(s) 662
MscI TGGCCA 1 cut(s) 662
MseI TTAA 2 cut(s) 63, 996
MslI CAYNNNNRTG 2 cut(s) 194, 836
Msp20I TGGCCA 1 cut(s) 662
MspCI CTTAAG 1 cut(s) 995
MspI CCGG 3 cut(s) 78, 165, 1037
MspR9I CCNGG 2 cut(s) 78, 596
MunI CAATTG 1 cut(s) 808
MvaI CCWGG 1 cut(s) 596
MwoI GCNNNNNNNGC 3 cut(s) 140, 335, 785
NciI CCSGG 1 cut(s) 78
NdeII GATC 7 cut(s) 279, 432, 616, 804, 840, 951, 1011
NlaIII CATG 3 cut(s) 199, 232, 887
NlaIV GGNNCC 3 cut(s) 82, 337, 1093
NmuCI GTSAC 3 cut(s) 31, 187, 398
NspV TTCGAA 1 cut(s) 762
PfeI GAWTC 2 cut(s) 69, 319
PkrI GCNGC 3 cut(s) 393, 550, 640
PleI GAGTC 1 cut(s) 970
PpsI GAGTC 1 cut(s) 970
Ppu21I YACGTR 1 cut(s) 932
Psp6I CCWGG 1 cut(s) 594
PspEI GGTNACC 1 cut(s) 398
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 1 cut(s) 594
PspN4I GGNNCC 3 cut(s) 82, 337, 1093
PspPI GGNCC 2 cut(s) 125, 346
PsrI GAACNNNNNNTAC 2 cut(s) 592, 624
PstI CTGCAG 1 cut(s) 550
PstNI CAGNNNCTG 1 cut(s) 752
PsuI RGATCY 1 cut(s) 279
RsaI GTAC 6 cut(s) 368, 376, 541, 610, 773, 1093
RsaNI GTAC 6 cut(s) 367, 375, 540, 609, 772, 1092
RseI CAYNNNNRTG 2 cut(s) 194, 836
SaqAI TTAA 2 cut(s) 63, 996
SatI GCNGC 3 cut(s) 392, 549, 639
Sau3AI GATC 7 cut(s) 279, 432, 616, 804, 840, 951, 1011
Sau96I GGNCC 2 cut(s) 125, 346
SchI GAGTC 1 cut(s) 971
ScrFI CCNGG 2 cut(s) 78, 596
SduI GDGCHC 2 cut(s) 746, 833
SfaNI GCATC 3 cut(s) 478, 703, 847
SfcI CTRYAG 1 cut(s) 546
SfuI TTCGAA 1 cut(s) 762
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 2 cut(s) 194, 836
SmlI CTYRAG 2 cut(s) 410, 995
SmoI CTYRAG 2 cut(s) 410, 995
SnaBI TACGTA 1 cut(s) 932
Sse9I AATT 4 cut(s) 60, 159, 808, 1051
SsiI CCGC 3 cut(s) 329, 567, 639
SspMI CTAG 2 cut(s) 9, 728
StyD4I CCNGG 2 cut(s) 76, 594
TaaI ACNGT 3 cut(s) 37, 776, 947
TaiI ACGT 1 cut(s) 934
TaqI TCGA 8 cut(s) 223, 317, 486, 579, 624, 762, 843, 1113
TasI AATT 4 cut(s) 60, 159, 808, 1051
TatI WGTACW 1 cut(s) 771
TauI GCSGC 1 cut(s) 641
TfiI GAWTC 2 cut(s) 69, 319
Tru1I TTAA 2 cut(s) 63, 996
Tru9I TTAA 2 cut(s) 63, 996
TscAI CASTG 5 cut(s) 42, 474, 757, 781, 1113
TseFI GTSAC 3 cut(s) 31, 187, 398
TseI GCWGC 2 cut(s) 391, 548
Tsp45I GTSAC 3 cut(s) 31, 187, 398
TspDTI ATGAA 3 cut(s) 249, 872, 1113
TspGWI ACGGA 1 cut(s) 998
TspRI CASTG 5 cut(s) 42, 474, 757, 781, 1113
Vha464I CTTAAG 1 cut(s) 995
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 1 cut(s) 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 1129
XspI CTAG 2 cut(s) 9, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.