RLG00000017377

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001289
Physical Location & Seq
Reverse (-)
15847190 .. 15848195
1006 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017377

Sequence Viewer

Length: 573 bp
ATGTCTACTGGTTTGGCAAAGAGAAAGATGGTGACAGTGTTAACTGATTTGGATGGCCCGAGTGCAAGACTTGCAGATTATTTTGATATAATCGCCGGAACTAGCACAGGTGGATTGGTCACCACCATGCTTACAGCTCCCAACAAGGACAATCGACCCATGTATGAAGCAAAGGACATCAATAACTTCTACTTGGAACAAACACCTAAGATTTTCCCTCAGAAGAGTCGAAAGAACTTCTTGACATCAATTCAAAGTTTGGTTGGAGCGGTGACCGGACCAAAATACGACGGGAATTGCCTGCGCACATTGACGACCGGGCAGCTTGGCGACCTCACAATAAAAGAAACTCTAACCAATGTCATCATCCCAACGTTCGATATCAAACACCTTCAGCCTGTGATTTTCTCAACCATTGATGCAAAAGAAAGTTCTTTGAAAAATGCAAAGCTTTCAGATATCTGCATCAGTACCTCTGCAGCTCCAACTTTTCTCCCCGGAACAACTCGTTCTTTTGATCTCGTTGATGGTGGGGTCGCTGCAAATAATCCTGTAAGTAATCTATGCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

191

Amino Acids

20.45

Weight (kDa)

8.41

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 186 1.8e-18 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 269
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 269
AclI AACGTT 1 cut(s) 374
AcuI CTGAAG 1 cut(s) 377
AfaI GTAC 1 cut(s) 472
AgsI TTSAA 2 cut(s) 254, 439
AluBI AGCT 4 cut(s) 137, 325, 451, 482
AluI AGCT 4 cut(s) 137, 325, 451, 482
Ama87I CYCGRG 1 cut(s) 58
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 3 cut(s) 322, 479, 539
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 56, 278
AsuC2I CCSGG 2 cut(s) 319, 498
AsuHPI GGTGA 3 cut(s) 43, 112, 283
AvaI CYCGRG 1 cut(s) 58
AvaII GGWCC 1 cut(s) 278
BbvI GCAGC 3 cut(s) 334, 491, 526
BccI CCATC 3 cut(s) 22, 47, 521
BcnI CCSGG 2 cut(s) 319, 498
BfaI CTAG 1 cut(s) 102
BfmI CTRYAG 1 cut(s) 477
BisI GCNGC 3 cut(s) 323, 480, 540
BlsI GCNGC 3 cut(s) 324, 481, 541
Bme1390I CCNGG 2 cut(s) 319, 498
Bme18I GGWCC 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 58
BmgT120I GGNCC 2 cut(s) 56, 278
BmrFI CCNGG 2 cut(s) 319, 498
BmsI GCATC 2 cut(s) 409, 474
BpuMI CCSGG 2 cut(s) 319, 498
BsaJI CCNNGG 1 cut(s) 496
BsaWI WCCGGW 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 13
BseDI CCNNGG 1 cut(s) 496
BseGI GGATG 2 cut(s) 58, 366
BseMII CTCAG 1 cut(s) 233
BseNI ACTGG 1 cut(s) 13
BseXI GCAGC 3 cut(s) 334, 491, 526
Bsh1285I CGRYCG 1 cut(s) 318
BshFI GGCC 1 cut(s) 57
BsiEI CGRYCG 1 cut(s) 318
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 4 cut(s) 96, 276, 318, 498
BsnI GGCC 1 cut(s) 57
BsoBI CYCGRG 1 cut(s) 58
Bsp143I GATC 1 cut(s) 517
BspACI CCGC 1 cut(s) 269
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 1 cut(s) 232
BspMAI CTGCAG 1 cut(s) 481
BsrBI CCGCTC 1 cut(s) 269
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 496
BssMI GATC 1 cut(s) 517
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 1 cut(s) 218
BstAPI GCANNNNNTGC 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 302
BstDEI CTNAG 2 cut(s) 207, 219
BstEII GGTNACC 2 cut(s) 118, 271
BstF5I GGATG 2 cut(s) 58, 366
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 1 cut(s) 520
BstMBI GATC 1 cut(s) 517
BstMCI CGRYCG 1 cut(s) 318
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstPI GGTNACC 2 cut(s) 118, 271
BstSCI CCNGG 2 cut(s) 317, 496
BstSFI CTRYAG 1 cut(s) 477
BstV1I GCAGC 3 cut(s) 334, 491, 526
BsuRI GGCC 1 cut(s) 57
BtsCI GGATG 2 cut(s) 58, 366
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 1 cut(s) 302
CfoI GCGC 1 cut(s) 306
Cfr13I GGNCC 2 cut(s) 56, 278
Csp6I GTAC 1 cut(s) 471
CviAII CATG 2 cut(s) 127, 160
CviJI RGCY 6 cut(s) 57, 137, 325, 397, 451, 482
CviKI_1 RGCY 6 cut(s) 57, 137, 325, 397, 451, 482
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 2 cut(s) 207, 219
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Eco32I GATATC 2 cut(s) 382, 460
Eco47I GGWCC 1 cut(s) 278
Eco57I CTGAAG 1 cut(s) 377
Eco88I CYCGRG 1 cut(s) 58
Eco91I GGTNACC 2 cut(s) 118, 271
EcoO65I GGTNACC 2 cut(s) 118, 271
EcoRV GATATC 2 cut(s) 382, 460
FaeI CATG 2 cut(s) 130, 163
FaiI YATR 5 cut(s) 89, 128, 161, 165, 565
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FatI CATG 2 cut(s) 126, 159
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 323, 480, 540
FokI GGATG 2 cut(s) 65, 353
Fsp4HI GCNGC 3 cut(s) 323, 480, 540
FspBI CTAG 1 cut(s) 102
FspI TGCGCA 1 cut(s) 305
GlaI GCGC 1 cut(s) 305
GluI GCNGC 3 cut(s) 323, 480, 540
HaeIII GGCC 1 cut(s) 57
HapII CCGG 4 cut(s) 96, 276, 318, 498
HhaI GCGC 1 cut(s) 306
Hin1II CATG 2 cut(s) 130, 163
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 1 cut(s) 226
HpaI GTTAAC 1 cut(s) 42
HpaII CCGG 4 cut(s) 96, 276, 318, 498
HphI GGTGA 3 cut(s) 43, 112, 283
Hpy166II GTNNAC 2 cut(s) 6, 42
Hpy188I TCNGA 2 cut(s) 222, 457
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 2 cut(s) 6, 42
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 1 cut(s) 401
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 7 cut(s) 65, 74, 422, 446, 465, 479, 542
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 2 cut(s) 207, 219
HpySE526I ACGT 1 cut(s) 374
Hsp92II CATG 2 cut(s) 130, 163
HspAI GCGC 1 cut(s) 304
KspAI GTTAAC 1 cut(s) 42
Kzo9I GATC 1 cut(s) 517
LmnI GCTCC 3 cut(s) 142, 266, 487
LpnPI CCDG 8 cut(s) 93, 109, 289, 314, 331, 411, 511, 564
Lsp1109I GCAGC 3 cut(s) 334, 491, 526
LweI GCATC 2 cut(s) 409, 474
MaeI CTAG 1 cut(s) 102
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 3 cut(s) 31, 118, 271
MalI GATC 1 cut(s) 519
MbiI CCGCTC 1 cut(s) 269
MboI GATC 1 cut(s) 517
MboII GAAGA 1 cut(s) 235
MluCI AATT 2 cut(s) 249, 295
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 2 cut(s) 244, 509
MnlI CCTC 3 cut(s) 228, 344, 484
MseI TTAA 1 cut(s) 41
MslI CAYNNNNRTG 1 cut(s) 125
MspI CCGG 4 cut(s) 96, 276, 318, 498
MspR9I CCNGG 2 cut(s) 319, 498
MwoI GCNNNNNNNGC 1 cut(s) 71
NciI CCSGG 2 cut(s) 319, 498
NdeII GATC 1 cut(s) 517
NlaIII CATG 2 cut(s) 130, 163
NmuCI GTSAC 3 cut(s) 31, 118, 271
NsbI TGCGCA 1 cut(s) 305
PkrI GCNGC 3 cut(s) 324, 481, 541
PleI GAGTC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 234
Psp1406I AACGTT 1 cut(s) 374
PspEI GGTNACC 2 cut(s) 118, 271
PspPI GGNCC 2 cut(s) 56, 278
PstI CTGCAG 1 cut(s) 481
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 1 cut(s) 125
SaqAI TTAA 1 cut(s) 41
SatI GCNGC 3 cut(s) 323, 480, 540
Sau3AI GATC 1 cut(s) 517
Sau96I GGNCC 2 cut(s) 56, 278
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 2 cut(s) 319, 498
SfaNI GCATC 2 cut(s) 409, 474
SfcI CTRYAG 1 cut(s) 477
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 125
Sse9I AATT 2 cut(s) 249, 295
SsiI CCGC 1 cut(s) 269
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 2 cut(s) 317, 496
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 1 cut(s) 377
TaqI TCGA 3 cut(s) 154, 229, 378
TasI AATT 2 cut(s) 249, 295
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 3 cut(s) 31, 118, 271
TseI GCWGC 3 cut(s) 322, 479, 539
Tsp45I GTSAC 3 cut(s) 31, 118, 271
TspDTI ATGAA 1 cut(s) 180
TspRI CASTG 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.