Lipolytic acyl hydrolase (LAH)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 130 |
| AcsI | RAATTY | 1 cut(s) | 309 |
| AcuI | CTGAAG | 1 cut(s) | 107 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 32 |
| AgsI | TTSAA | 2 cut(s) | 296, 379 |
| AjnI | CCWGG | 1 cut(s) | 88 |
| AluBI | AGCT | 2 cut(s) | 121, 327 |
| AluI | AGCT | 2 cut(s) | 121, 327 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 148 |
| AoxI | GGCC | 1 cut(s) | 211 |
| ApeKI | GCWGC | 1 cut(s) | 266 |
| ApoI | RAATTY | 1 cut(s) | 309 |
| BbsI | GAAGAC | 2 cut(s) | 8, 397 |
| BbvI | GCAGC | 1 cut(s) | 253 |
| BccI | CCATC | 3 cut(s) | 56, 146, 398 |
| BciT130I | CCWGG | 1 cut(s) | 90 |
| BfaI | CTAG | 1 cut(s) | 201 |
| BisI | GCNGC | 1 cut(s) | 267 |
| BlsI | GCNGC | 1 cut(s) | 268 |
| Bme1390I | CCNGG | 1 cut(s) | 90 |
| BmrFI | CCNGG | 1 cut(s) | 90 |
| BmsI | GCATC | 1 cut(s) | 151 |
| BpiI | GAAGAC | 2 cut(s) | 8, 397 |
| BpmI | CTGGAG | 1 cut(s) | 131 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 32 |
| Bse1I | ACTGG | 1 cut(s) | 153 |
| BseBI | CCWGG | 1 cut(s) | 90 |
| BseGI | GGATG | 2 cut(s) | 157, 166 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 32 |
| BseMII | CTCAG | 1 cut(s) | 115 |
| BseNI | ACTGG | 1 cut(s) | 153 |
| BseXI | GCAGC | 1 cut(s) | 253 |
| BshFI | GGCC | 1 cut(s) | 213 |
| BslFI | GGGAC | 2 cut(s) | 211, 240 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 32 |
| BsmFI | GGGAC | 2 cut(s) | 211, 240 |
| BsnI | GGCC | 1 cut(s) | 213 |
| BspACI | CCGC | 1 cut(s) | 130 |
| BspANI | GGCC | 1 cut(s) | 213 |
| BspCNI | CTCAG | 1 cut(s) | 114 |
| BsrI | ACTGG | 1 cut(s) | 153 |
| Bst2UI | CCWGG | 1 cut(s) | 90 |
| Bst4CI | ACNGT | 2 cut(s) | 49, 347 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 170 |
| BstC8I | GCNNGC | 1 cut(s) | 171 |
| BstDEI | CTNAG | 4 cut(s) | 30, 53, 101, 369 |
| BstF5I | GGATG | 2 cut(s) | 157, 166 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 127, 170, 210 |
| BstNI | CCWGG | 1 cut(s) | 90 |
| BstSCI | CCNGG | 1 cut(s) | 88 |
| BstV1I | GCAGC | 1 cut(s) | 253 |
| BstV2I | GAAGAC | 2 cut(s) | 8, 397 |
| BsuRI | GGCC | 1 cut(s) | 213 |
| BtsCI | GGATG | 2 cut(s) | 157, 166 |
| BtsIMutI | CAGTG | 1 cut(s) | 45 |
| Cac8I | GCNNGC | 1 cut(s) | 171 |
| CaiI | CAGNNNCTG | 1 cut(s) | 148 |
| CviAII | CATG | 2 cut(s) | 12, 226 |
| CviJI | RGCY | 6 cut(s) | 39, 121, 137, 169, 213, 327 |
| CviKI_1 | RGCY | 6 cut(s) | 39, 121, 137, 169, 213, 327 |
| DdeI | CTNAG | 4 cut(s) | 30, 53, 101, 369 |
| Eco57I | CTGAAG | 1 cut(s) | 107 |
| EcoRII | CCWGG | 1 cut(s) | 88 |
| FaeI | CATG | 2 cut(s) | 15, 229 |
| FaiI | YATR | 6 cut(s) | 13, 98, 140, 227, 264, 402 |
| FaqI | GGGAC | 2 cut(s) | 211, 240 |
| FatI | CATG | 2 cut(s) | 11, 225 |
| Fnu4HI | GCNGC | 1 cut(s) | 267 |
| FokI | GGATG | 2 cut(s) | 164, 173 |
| Fsp4HI | GCNGC | 1 cut(s) | 267 |
| FspBI | CTAG | 1 cut(s) | 201 |
| GluI | GCNGC | 1 cut(s) | 267 |
| GsuI | CTGGAG | 1 cut(s) | 131 |
| HaeIII | GGCC | 1 cut(s) | 213 |
| Hin1II | CATG | 2 cut(s) | 15, 229 |
| HinfI | GANTC | 1 cut(s) | 113 |
| Hpy188I | TCNGA | 1 cut(s) | 118 |
| Hpy188III | TCNNGA | 2 cut(s) | 110, 293 |
| HpyCH4III | ACNGT | 2 cut(s) | 49, 347 |
| HpyCH4V | TGCA | 3 cut(s) | 142, 164, 173 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 127, 170, 210 |
| HpyF3I | CTNAG | 4 cut(s) | 30, 53, 101, 369 |
| Hsp92II | CATG | 2 cut(s) | 15, 229 |
| LpnPI | CCDG | 8 cut(s) | 75, 95, 102, 110, 134, 180, 192, 252 |
| Lsp1109I | GCAGC | 1 cut(s) | 253 |
| LweI | GCATC | 1 cut(s) | 151 |
| MaeI | CTAG | 1 cut(s) | 201 |
| MaeIII | GTNAC | 1 cut(s) | 217 |
| MboII | GAAGA | 2 cut(s) | 8, 402 |
| MluCI | AATT | 1 cut(s) | 309 |
| MlyI | GAGTC | 1 cut(s) | 122 |
| MnlI | CCTC | 4 cut(s) | 50, 59, 71, 374 |
| MseI | TTAA | 2 cut(s) | 279, 339 |
| MspR9I | CCNGG | 1 cut(s) | 90 |
| MvaI | CCWGG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 127, 170, 210 |
| NlaIII | CATG | 2 cut(s) | 15, 229 |
| PfoI | TCCNGGA | 1 cut(s) | 88 |
| PkrI | GCNGC | 1 cut(s) | 268 |
| PleI | GAGTC | 1 cut(s) | 121 |
| PpsI | GAGTC | 1 cut(s) | 121 |
| Psp6I | CCWGG | 1 cut(s) | 88 |
| PspGI | CCWGG | 1 cut(s) | 88 |
| PstNI | CAGNNNCTG | 1 cut(s) | 148 |
| SaqAI | TTAA | 2 cut(s) | 279, 339 |
| SatI | GCNGC | 1 cut(s) | 267 |
| SchI | GAGTC | 1 cut(s) | 122 |
| ScrFI | CCNGG | 1 cut(s) | 90 |
| SetI | ASST | 5 cut(s) | 70, 123, 211, 329, 366 |
| SfaNI | GCATC | 1 cut(s) | 151 |
| Sse9I | AATT | 1 cut(s) | 309 |
| SsiI | CCGC | 1 cut(s) | 130 |
| SspMI | CTAG | 1 cut(s) | 201 |
| StyD4I | CCNGG | 1 cut(s) | 88 |
| TaaI | ACNGT | 2 cut(s) | 49, 347 |
| TasI | AATT | 1 cut(s) | 309 |
| Tru1I | TTAA | 2 cut(s) | 279, 339 |
| Tru9I | TTAA | 2 cut(s) | 279, 339 |
| TscAI | CASTG | 1 cut(s) | 52 |
| TseI | GCWGC | 1 cut(s) | 266 |
| TspDTI | ATGAA | 1 cut(s) | 258 |
| TspGWI | ACGGA | 1 cut(s) | 412 |
| TspRI | CASTG | 1 cut(s) | 52 |
| XapI | RAATTY | 1 cut(s) | 309 |
| XspI | CTAG | 1 cut(s) | 201 |