Rh2BG181700

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001289
Physical Location & Seq
Reverse (-)
16190689 .. 16192718
2030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG181700.1

Sequence Viewer

Length: 1116 bp
ATGTCCATTGGTTTGGCAAAGAGAAAGATGGTGACAGTTCTGAGCATTGATGGAGGTGGCATTAGAGGCATAATTCCCGGCACCATCCTCGGCTTTCTCGAATCTAAGCTTCAGGATTGGGATGGAGCAAATGCAAGAATTGCAGATTATTTTGACATAATCGCCGGAACTAGCACCGGGGGATTGGTCACCACCATGCTTACAGCTCCCAGTAAGAACAACCGGCCCATGTATGAAGCAAAGGACATCAACAACTTTTATTTAGGACACTGTCCCCAGATTTTCCCTCAAAAAAGTCGGAAAAAGAAAAACTTCTTCACATCGATAACAAATGCGATGAAGCGGGGGCCAAAATACGATGGGATGTATCTGCGCTCATTGATAAACGGGCTCCTTGGTGATCTCACTCTCAAGCAGACTTTAACCAATGTGGTCATTCCAAGTTTTGATATCAAACACCTTCAGCCTGTGGTTTTCTCAACCATCGATGCAAAAGAAAATTCATTGAAGAACGCAAAGCTTTCAGATGTCTGCATCAGTACCTCTGCAGCTCCATATTATCTCCCTCCACACTACTTCGAAATCAAGGATGACCAGGGAAATATCCGCAAATTTGATCTTGTCGATGTAATTTACCGTAGTGCTGCAATTAAGGAACTAATGCATGGATTTTATTCCATGAATGAAAGACAATGCCATAAGCCACATAAACAGAGAGATGTTGAAGAACTACTCAGAACCAATAAATGCGAATCGCTTGCAAGTGCTTTTAATAATGGGAGCACACCTTTGCTGGACATTTTTGGGGATGCAAGTTCTGATATGGTTGACTTTCATGTCTCCACTCTCTTCCAGTCCCTCCATGTCAAGGACAATTACCTACGAATTCAGGATGACACACTGAGCGGAGATGAATCATCAGTGGATATTGCAACCGAGAAGAATCTGAAAAGCCTTGTGGAGATCGGAAATGTGTTGCTGAAGAAGCCAGTGTCAAGGGTAAATCTCGATACTGGGAGGTACGAGGAATCTGAGGGAGACGGTACCTACGAAGAAGCACTGGTCGATTTTGCCAAACGGCTCGCGGAGGGAAAGAAGCTCGGGCAAAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

371

Amino Acids

41.35

Weight (kDa)

7.65

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 14 - 194 2.7e-22 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 836
Acc65I GGTACC 1 cut(s) 1043
AccB1I GGYRCC 2 cut(s) 80, 1043
AccBSI CCGCTC 1 cut(s) 906
AccII CGCG 1 cut(s) 1085
AciI CCGC 4 cut(s) 343, 607, 906, 1085
AcsI RAATTY 3 cut(s) 499, 611, 885
AcuI CTGAAG 3 cut(s) 95, 446, 1001
AfaI GTAC 3 cut(s) 541, 1022, 1045
AfiI CCNNNNNNNGG 1 cut(s) 868
AgsI TTSAA 2 cut(s) 508, 725
AjnI CCWGG 1 cut(s) 594
AluBI AGCT 5 cut(s) 109, 206, 520, 551, 1099
AluI AGCT 5 cut(s) 109, 206, 520, 551, 1099
Alw21I GWGCWC 1 cut(s) 785
Alw26I GTCTC 2 cut(s) 844, 1032
Ama87I CYCGRG 1 cut(s) 1100
AoxI GGCC 2 cut(s) 224, 347
ApeKI GCWGC 2 cut(s) 548, 644
ApoI RAATTY 3 cut(s) 499, 611, 885
Asp700I GAANNNNTTC 1 cut(s) 311
Asp718I GGTACC 1 cut(s) 1043
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 2 cut(s) 225, 347
AsuC2I CCSGG 2 cut(s) 78, 178
AsuHPI GGTGA 3 cut(s) 43, 181, 410
AsuII TTCGAA 1 cut(s) 579
AvaI CYCGRG 1 cut(s) 1100
BanI GGYRCC 2 cut(s) 80, 1043
BanII GRGCYC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 785
BbvI GCAGC 2 cut(s) 560, 631
BccI CCATC 6 cut(s) 22, 44, 92, 116, 353, 491
BceAI ACGGC 1 cut(s) 1094
BcgI CGANNNNNNTGC 4 cut(s) 70, 104, 740, 774
BciT130I CCWGG 1 cut(s) 596
BcnI CCSGG 2 cut(s) 78, 178
BcoDI GTCTC 2 cut(s) 844, 1032
BfaI CTAG 1 cut(s) 171
BfmI CTRYAG 1 cut(s) 546
BisI GCNGC 2 cut(s) 549, 645
BlsI GCNGC 2 cut(s) 550, 646
Bme1390I CCNGG 3 cut(s) 78, 178, 596
BmeT110I CYCGRG 1 cut(s) 1100
BmgT120I GGNCC 2 cut(s) 225, 347
BmiI GGNNCC 4 cut(s) 82, 348, 392, 1045
BmrFI CCNGG 3 cut(s) 78, 178, 596
BmrI ACTGGG 2 cut(s) 204, 1023
BmsI GCATC 3 cut(s) 478, 543, 799
BmuI ACTGGG 2 cut(s) 204, 1023
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 2 cut(s) 78, 178
Bsa29I ATCGAT 2 cut(s) 323, 486
BsaJI CCNNGG 4 cut(s) 88, 177, 394, 595
Bsc4I CCNNNNNNNGG 1 cut(s) 868
Bse118I RCCGGY 1 cut(s) 222
Bse1I ACTGG 5 cut(s) 210, 853, 989, 1018, 1065
BseBI CCWGG 1 cut(s) 596
BseCI ATCGAT 2 cut(s) 323, 486
BseDI CCNNGG 4 cut(s) 88, 177, 394, 595
BseGI GGATG 6 cut(s) 84, 127, 369, 595, 814, 898
BseLI CCNNNNNNNGG 1 cut(s) 868
BseMII CTCAG 4 cut(s) 32, 748, 893, 1023
BseNI ACTGG 5 cut(s) 210, 853, 989, 1018, 1065
BseXI GCAGC 2 cut(s) 560, 631
Bsh1236I CGCG 1 cut(s) 1085
BshFI GGCC 2 cut(s) 226, 349
BshNI GGYRCC 2 cut(s) 80, 1043
BshVI ATCGAT 2 cut(s) 323, 486
BsiHKAI GWGCWC 1 cut(s) 785
BsiHKCI CYCGRG 1 cut(s) 1100
BsiSI CCGG 4 cut(s) 78, 165, 177, 223
BslFI GGGAC 2 cut(s) 258, 841
BslI CCNNNNNNNGG 1 cut(s) 868
BsmAI GTCTC 2 cut(s) 844, 1032
BsmBI CGTCTC 1 cut(s) 1032
BsmFI GGGAC 2 cut(s) 258, 841
BsnI GGCC 2 cut(s) 226, 349
BsoBI CYCGRG 1 cut(s) 1100
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 2 cut(s) 393, 785
Bsp143I GATC 3 cut(s) 400, 616, 963
BspACI CCGC 4 cut(s) 343, 607, 906, 1085
BspANI GGCC 2 cut(s) 226, 349
BspCNI CTCAG 4 cut(s) 33, 747, 894, 1024
BspDI ATCGAT 2 cut(s) 323, 486
BspFNI CGCG 1 cut(s) 1085
BspLI GGNNCC 4 cut(s) 82, 348, 392, 1045
BspMAI CTGCAG 1 cut(s) 550
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 2 cut(s) 80, 1043
BsrBI CCGCTC 1 cut(s) 906
BsrFI RCCGGY 1 cut(s) 222
BsrI ACTGG 5 cut(s) 210, 853, 989, 1018, 1065
BssAI RCCGGY 1 cut(s) 222
BssECI CCNNGG 4 cut(s) 88, 177, 394, 595
BssMI GATC 3 cut(s) 400, 616, 963
BssT1I CCWWGG 1 cut(s) 394
Bst2UI CCWGG 1 cut(s) 596
Bst4CI ACNGT 4 cut(s) 37, 272, 638, 1043
Bst6I CTCTTC 1 cut(s) 854
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 2 cut(s) 759, 1083
BstDEI CTNAG 5 cut(s) 41, 105, 734, 902, 1032
BstEII GGTNACC 1 cut(s) 187
BstF5I GGATG 6 cut(s) 84, 127, 369, 595, 814, 898
BstFNI CGCG 1 cut(s) 1085
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 3 cut(s) 403, 619, 966
BstMAI GTCTC 2 cut(s) 844, 1032
BstMBI GATC 3 cut(s) 400, 616, 963
BstMWI GCNNNNNNNGC 3 cut(s) 66, 140, 985
BstNI CCWGG 1 cut(s) 596
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 3 cut(s) 76, 176, 594
BstSFI CTRYAG 1 cut(s) 546
BstUI CGCG 1 cut(s) 1085
BstV1I GCAGC 2 cut(s) 560, 631
BstXI CCANNNNNNTGG 1 cut(s) 13
Bsu15I ATCGAT 2 cut(s) 323, 486
BsuRI GGCC 2 cut(s) 226, 349
BsuTUI ATCGAT 2 cut(s) 323, 486
BtgZI GCGATG 1 cut(s) 350
BtsCI GGATG 6 cut(s) 84, 127, 369, 595, 814, 898
BtsIMutI CAGTG 5 cut(s) 268, 899, 927, 996, 1058
Cac8I GCNNGC 2 cut(s) 759, 1083
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 2 cut(s) 225, 347
ClaI ATCGAT 2 cut(s) 323, 486
Csp6I GTAC 3 cut(s) 540, 1021, 1044
CviAII CATG 6 cut(s) 196, 229, 665, 679, 836, 863
CviQI GTAC 3 cut(s) 540, 1021, 1044
DdeI CTNAG 5 cut(s) 41, 105, 734, 902, 1032
DpnI GATC 3 cut(s) 402, 618, 965
DpnII GATC 3 cut(s) 400, 616, 963
DrdI GACNNNNNNGTC 1 cut(s) 836
DseDI GACNNNNNNGTC 1 cut(s) 836
Eam1104I CTCTTC 1 cut(s) 854
EarI CTCTTC 1 cut(s) 854
Eco130I CCWWGG 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 393
Eco32I GATATC 1 cut(s) 451
Eco57I CTGAAG 3 cut(s) 95, 446, 1001
Eco88I CYCGRG 1 cut(s) 1100
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
EcoRI GAATTC 1 cut(s) 885
EcoRII CCWGG 1 cut(s) 594
EcoRV GATATC 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 394
EcoT22I ATGCAT 1 cut(s) 666
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 394
Esp3I CGTCTC 1 cut(s) 1032
FaeI CATG 6 cut(s) 199, 232, 668, 682, 839, 866
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 2 cut(s) 258, 841
FatI CATG 6 cut(s) 195, 228, 664, 678, 835, 862
FauI CCCGC 1 cut(s) 336
Fnu4HI GCNGC 2 cut(s) 549, 645
FokI GGATG 6 cut(s) 71, 134, 376, 602, 821, 905
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 2 cut(s) 549, 645
FspBI CTAG 1 cut(s) 171
GlaI GCGC 1 cut(s) 374
GluI GCNGC 2 cut(s) 549, 645
HaeIII GGCC 2 cut(s) 226, 349
HapII CCGG 4 cut(s) 78, 165, 177, 223
HhaI GCGC 1 cut(s) 375
Hin1II CATG 6 cut(s) 199, 232, 668, 682, 839, 866
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 829
HindII GTYRAC 1 cut(s) 829
HindIII AAGCTT 2 cut(s) 107, 518
HinfI GANTC 5 cut(s) 101, 752, 914, 943, 1028
HpaII CCGG 4 cut(s) 78, 165, 177, 223
HphI GGTGA 3 cut(s) 43, 181, 410
Hpy166II GTNNAC 1 cut(s) 829
Hpy188I TCNGA 8 cut(s) 42, 300, 526, 737, 820, 948, 968, 1033
Hpy188III TCNNGA 4 cut(s) 98, 113, 890, 1007
Hpy8I GTNNAC 1 cut(s) 829
HpyAV CCTTC 1 cut(s) 470
HpyCH4III ACNGT 4 cut(s) 37, 272, 638, 1043
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 140, 985
HpyF3I CTNAG 5 cut(s) 41, 105, 734, 902, 1032
Hsp92II CATG 6 cut(s) 199, 232, 668, 682, 839, 866
HspAI GCGC 1 cut(s) 373
KpnI GGTACC 1 cut(s) 1047
Kzo9I GATC 3 cut(s) 400, 616, 963
LmnI GCTCC 5 cut(s) 125, 211, 396, 556, 780
Lsp1109I GCAGC 2 cut(s) 560, 631
LweI GCATC 3 cut(s) 478, 543, 799
MaeI CTAG 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 31, 187
MalI GATC 3 cut(s) 402, 618, 965
MbiI CCGCTC 1 cut(s) 906
MboI GATC 3 cut(s) 400, 616, 963
MboII GAAGA 7 cut(s) 307, 520, 737, 841, 952, 994, 1064
MhlI GDGCHC 2 cut(s) 393, 785
MluCI AATT 9 cut(s) 72, 138, 499, 611, 630, 648, 874, 885, 1111
MmeI TCCRAC 1 cut(s) 278
Mph1103I ATGCAT 1 cut(s) 666
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 3 cut(s) 422, 651, 771
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 4 cut(s) 78, 165, 177, 223
MspR9I CCNGG 3 cut(s) 78, 178, 596
MvaI CCWGG 1 cut(s) 596
MvnI CGCG 1 cut(s) 1085
MwoI GCNNNNNNNGC 3 cut(s) 66, 140, 985
NciI CCSGG 2 cut(s) 78, 178
NdeII GATC 3 cut(s) 400, 616, 963
NlaIII CATG 6 cut(s) 199, 232, 668, 682, 839, 866
NlaIV GGNNCC 4 cut(s) 82, 348, 392, 1045
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 31, 187
NsiI ATGCAT 1 cut(s) 666
NspV TTCGAA 1 cut(s) 579
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 1047
PdmI GAANNNNTTC 1 cut(s) 311
PfeI GAWTC 5 cut(s) 101, 752, 914, 943, 1028
PflFI GACNNNGTC 1 cut(s) 270
PkrI GCNGC 2 cut(s) 550, 646
Psp6I CCWGG 1 cut(s) 594
PspEI GGTNACC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 594
PspN4I GGNNCC 4 cut(s) 82, 348, 392, 1045
PspPI GGNCC 2 cut(s) 225, 347
PstI CTGCAG 1 cut(s) 550
PsyI GACNNNGTC 1 cut(s) 270
RsaI GTAC 3 cut(s) 541, 1022, 1045
RsaNI GTAC 3 cut(s) 540, 1021, 1044
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 3 cut(s) 422, 651, 771
SatI GCNGC 2 cut(s) 549, 645
Sau3AI GATC 3 cut(s) 400, 616, 963
Sau96I GGNCC 2 cut(s) 225, 347
ScrFI CCNGG 3 cut(s) 78, 178, 596
SduI GDGCHC 2 cut(s) 393, 785
SfaNI GCATC 3 cut(s) 478, 543, 799
SfcI CTRYAG 1 cut(s) 546
SfuI TTCGAA 1 cut(s) 579
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 9 cut(s) 72, 138, 499, 611, 630, 648, 874, 885, 1111
SsiI CCGC 4 cut(s) 343, 607, 906, 1085
SspMI CTAG 1 cut(s) 171
StyD4I CCNGG 3 cut(s) 76, 176, 594
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 4 cut(s) 37, 272, 638, 1043
TaqI TCGA 7 cut(s) 99, 323, 486, 579, 624, 1008, 1065
TasI AATT 9 cut(s) 72, 138, 499, 611, 630, 648, 874, 885, 1111
TfiI GAWTC 5 cut(s) 101, 752, 914, 943, 1028
Tru1I TTAA 3 cut(s) 422, 651, 771
Tru9I TTAA 3 cut(s) 422, 651, 771
TscAI CASTG 5 cut(s) 275, 906, 927, 996, 1065
TseFI GTSAC 2 cut(s) 31, 187
TseI GCWGC 2 cut(s) 548, 644
Tsp45I GTSAC 2 cut(s) 31, 187
TspDTI ATGAA 7 cut(s) 249, 353, 492, 695, 699, 824, 927
TspRI CASTG 5 cut(s) 275, 906, 927, 996, 1065
Tth111I GACNNNGTC 1 cut(s) 270
XapI RAATTY 3 cut(s) 499, 611, 885
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 1 cut(s) 171
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.