Lipolytic acyl hydrolase (LAH)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 118 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 237 |
| AgsI | TTSAA | 1 cut(s) | 98 |
| AoxI | GGCC | 1 cut(s) | 239 |
| ApeKI | GCWGC | 1 cut(s) | 131 |
| AspS9I | GGNCC | 1 cut(s) | 239 |
| AsuHPI | GGTGA | 1 cut(s) | 32 |
| BauI | CACGAG | 1 cut(s) | 72 |
| BbvI | GCAGC | 1 cut(s) | 118 |
| BccI | CCATC | 1 cut(s) | 11 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 38, 72 |
| BfaI | CTAG | 2 cut(s) | 80, 104 |
| BisI | GCNGC | 1 cut(s) | 132 |
| BlsI | GCNGC | 1 cut(s) | 133 |
| BmgT120I | GGNCC | 1 cut(s) | 239 |
| BmiI | GGNNCC | 1 cut(s) | 204 |
| BmsI | GCATC | 1 cut(s) | 16 |
| BsaJI | CCNNGG | 1 cut(s) | 135 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 237 |
| Bse1I | ACTGG | 1 cut(s) | 43 |
| BseDI | CCNNGG | 1 cut(s) | 135 |
| BseGI | GGATG | 1 cut(s) | 31 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 237 |
| BseNI | ACTGG | 1 cut(s) | 43 |
| BseXI | GCAGC | 1 cut(s) | 118 |
| BshFI | GGCC | 1 cut(s) | 241 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 237 |
| BsnI | GGCC | 1 cut(s) | 241 |
| BspACI | CCGC | 1 cut(s) | 118 |
| BspANI | GGCC | 1 cut(s) | 241 |
| BspLI | GGNNCC | 1 cut(s) | 204 |
| BsrI | ACTGG | 1 cut(s) | 43 |
| BssECI | CCNNGG | 1 cut(s) | 135 |
| BssSI | CACGAG | 1 cut(s) | 72 |
| BssT1I | CCWWGG | 1 cut(s) | 135 |
| Bst2BI | CACGAG | 1 cut(s) | 72 |
| BstC8I | GCNNGC | 1 cut(s) | 87 |
| BstF5I | GGATG | 1 cut(s) | 31 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 91 |
| BstV1I | GCAGC | 1 cut(s) | 118 |
| BsuRI | GGCC | 1 cut(s) | 241 |
| BtsCI | GGATG | 1 cut(s) | 31 |
| BtsI | GCAGTG | 1 cut(s) | 163 |
| BtsIMutI | CAGTG | 2 cut(s) | 36, 163 |
| Cac8I | GCNNGC | 1 cut(s) | 87 |
| Cfr13I | GGNCC | 1 cut(s) | 239 |
| CviJI | RGCY | 3 cut(s) | 42, 230, 241 |
| CviKI_1 | RGCY | 3 cut(s) | 42, 230, 241 |
| Eco130I | CCWWGG | 1 cut(s) | 135 |
| EcoT14I | CCWWGG | 1 cut(s) | 135 |
| ErhI | CCWWGG | 1 cut(s) | 135 |
| FaiI | YATR | 1 cut(s) | 92 |
| Fnu4HI | GCNGC | 1 cut(s) | 132 |
| FokI | GGATG | 1 cut(s) | 38 |
| Fsp4HI | GCNGC | 1 cut(s) | 132 |
| FspBI | CTAG | 2 cut(s) | 80, 104 |
| GluI | GCNGC | 1 cut(s) | 132 |
| HaeIII | GGCC | 1 cut(s) | 241 |
| HinfI | GANTC | 1 cut(s) | 33 |
| HphI | GGTGA | 1 cut(s) | 32 |
| Hpy188I | TCNGA | 1 cut(s) | 207 |
| HpyCH4V | TGCA | 3 cut(s) | 7, 29, 59 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 91 |
| LpnPI | CCDG | 4 cut(s) | 24, 45, 99, 197 |
| Lsp1109I | GCAGC | 1 cut(s) | 118 |
| LweI | GCATC | 1 cut(s) | 16 |
| MaeI | CTAG | 2 cut(s) | 80, 104 |
| MboII | GAAGA | 1 cut(s) | 169 |
| MnlI | CCTC | 2 cut(s) | 16, 167 |
| MseI | TTAA | 1 cut(s) | 144 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 91 |
| NlaIV | GGNNCC | 1 cut(s) | 204 |
| PfeI | GAWTC | 1 cut(s) | 33 |
| PkrI | GCNGC | 1 cut(s) | 133 |
| PspN4I | GGNNCC | 1 cut(s) | 204 |
| PspPI | GGNCC | 1 cut(s) | 239 |
| SaqAI | TTAA | 1 cut(s) | 144 |
| SatI | GCNGC | 1 cut(s) | 132 |
| Sau96I | GGNCC | 1 cut(s) | 239 |
| SetI | ASST | 1 cut(s) | 159 |
| SfaNI | GCATC | 1 cut(s) | 16 |
| SsiI | CCGC | 1 cut(s) | 118 |
| SspI | AATATT | 1 cut(s) | 191 |
| SspMI | CTAG | 2 cut(s) | 80, 104 |
| StyI | CCWWGG | 1 cut(s) | 135 |
| TaqI | TCGA | 1 cut(s) | 48 |
| TfiI | GAWTC | 1 cut(s) | 33 |
| Tru1I | TTAA | 1 cut(s) | 144 |
| Tru9I | TTAA | 1 cut(s) | 144 |
| TscAI | CASTG | 2 cut(s) | 43, 170 |
| TseI | GCWGC | 1 cut(s) | 131 |
| TspDTI | ATGAA | 1 cut(s) | 227 |
| TspRI | CASTG | 2 cut(s) | 43, 170 |
| XspI | CTAG | 2 cut(s) | 80, 104 |