Prupe.7G137200_v2.0.a1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001289
Physical Location & Seq
Forward (+)
15411420 .. 15413717
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G137200.1

Sequence Viewer

Length: 1155 bp
ATGTCGACGGACTTGGTAAAGAGAAAGATGGTGACAGTGTTGAGCATTGATGGAGGTGGAGTTAGAGGCATAATTCCCAGCACCATCCTGGCCTTTCTTGAATCCAAGCTTCAGCAATTGGATGGCCCCGATGCAAGAATTGCAGATTATTTCGACATAATTGCCGGAACAAGCACCGGTGGTCTTGTCACCACCATGCTTACAGCTCCGAACAAGGACAACAGACCCATGTATGAAGCCAAGGACATCAATAAATTCTATCTAGACCAGAGTCCTAAGATTTTTCCTCAGAGCAGTCGAAATAACTTATTGGGATCAATAAAAAGTATGGTTGACGAGGTAAAGGGGCCAAAATATGATGGGAAGTACCTGCGCACATTGACTCATGGGCTGCTTGGTGATCTCACTCTCAAACAGACTCTAACCAATGTGGTTATACCAGCTTTTGATATCAAGTACCTTCAGCCTGTGGTCTTCTCAACCATTGATGCAAAAGAAAGTAGTTTGAAGAATGCCAAGCTTTCGGATGTTTGCGTCAGTACCTCTGCAGCTCCCACTTTTCTCCCGGCACACTACTTCGAGGTCAAGGATGACGCGGGAAGGACTCGCACTTTTAATCTTGTTGATGGTGGGGTTGCCGCTAACAATCCTACAATGATGGCCATAAGCCACATAAACAGAGAGATGTTGAAGCACAACTCAGATCCGATAGATGCGACGAGGTTGCAGGTGCTATCATTGGGCACAGGTGCAGCCAAGCTTGAAGAGAAGTACAGTGCAGCCATAGCCTCCAAATGGGGTTTGATCAATTGGCTGTTTCACAATGGGAGCACACCTTTGGTAGACTTTTTTGCCGACGCAAGTTCTGATATGGTTGACTTTCATGTGTCCACCCTCTTCCAATGCCTCCAGGCCAAGGACAACTATCTACGTATTCAGGATGACTCGTTGACCGGCGACGAAGCGTCGCTTGATATTGCAACAGAGAAGAATCTAAGAAGGCTTGTGGAGATTGGAAATGCACTGTTGAAGAAGCCAGTGTCACGGGTGCAATTGGATACTGGAAAGTATGAGGAATGTGAAGGAGAAGGCACCTATGAAGATGCTCTGATCAAGTTTGCTAAAACGATCTCAGAGGGAAGGAAGCTCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

385

Amino Acids

42.21

Weight (kDa)

6.12

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 718
Acc16I TGCGCA 1 cut(s) 374
Acc36I ACCTGC 2 cut(s) 378, 718
AccB1I GGYRCC 1 cut(s) 1091
AccI GTMKAC 2 cut(s) 5, 843
AccII CGCG 1 cut(s) 596
AciI CCGC 2 cut(s) 596, 639
AclWI GGATC 2 cut(s) 322, 698
AcoI YGGCCR 1 cut(s) 660
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 2 cut(s) 95, 446
AfaI GTAC 4 cut(s) 368, 458, 541, 773
AfiI CCNNNNNNNGG 2 cut(s) 795, 916
AgeI ACCGGT 1 cut(s) 176
AgsI TTSAA 5 cut(s) 101, 508, 691, 764, 1030
AhdI GACNNNNNGTC 1 cut(s) 964
AjnI CCWGG 2 cut(s) 87, 909
AluBI AGCT 7 cut(s) 109, 206, 443, 520, 551, 760, 1147
AluI AGCT 7 cut(s) 109, 206, 443, 520, 551, 760, 1147
Alw21I GWGCWC 1 cut(s) 833
AlwI GGATC 2 cut(s) 322, 698
AoxI GGCC 5 cut(s) 90, 124, 347, 660, 912
ApeKI GCWGC 4 cut(s) 391, 548, 752, 779
ApoI RAATTY 1 cut(s) 254
AsiGI ACCGGT 1 cut(s) 176
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 2 cut(s) 125, 347
AsuC2I CCSGG 1 cut(s) 566
AsuHPI GGTGA 3 cut(s) 43, 181, 410
BaeGI GKGCMC 1 cut(s) 746
BalI TGGCCA 1 cut(s) 662
BanI GGYRCC 1 cut(s) 1091
BbsI GAAGAC 1 cut(s) 466
Bbv12I GWGCWC 1 cut(s) 833
BbvI GCAGC 4 cut(s) 378, 560, 764, 791
BccI CCATC 7 cut(s) 22, 44, 92, 116, 353, 620, 652
BcgI CGANNNNNNTGC 4 cut(s) 143, 177, 706, 740
BciT130I CCWGG 2 cut(s) 89, 911
BciVI GTATCC 1 cut(s) 1051
BclI TGATCA 2 cut(s) 804, 1110
BcnI CCSGG 1 cut(s) 566
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 1 cut(s) 546
BfuAI ACCTGC 2 cut(s) 378, 718
BfuI GTATCC 1 cut(s) 1051
BisI GCNGC 5 cut(s) 392, 549, 639, 753, 780
BlsI GCNGC 5 cut(s) 393, 550, 640, 754, 781
Bme1390I CCNGG 3 cut(s) 89, 566, 911
BmeRI GACNNNNNGTC 1 cut(s) 964
BmgT120I GGNCC 2 cut(s) 125, 347
BmiI GGNNCC 3 cut(s) 127, 348, 1093
BmrFI CCNGG 3 cut(s) 89, 566, 911
BmsI GCATC 4 cut(s) 121, 478, 703, 1093
BoxI GACNNNNGTC 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 466
BpmI CTGGAG 1 cut(s) 893
BpuMI CCSGG 1 cut(s) 566
BsaAI YACGTR 1 cut(s) 932
BsaJI CCNNGG 2 cut(s) 240, 915
BsaWI WCCGGW 1 cut(s) 176
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 2 cut(s) 795, 916
Bse118I RCCGGY 2 cut(s) 176, 953
Bse1I ACTGG 2 cut(s) 1037, 1066
BseBI CCWGG 2 cut(s) 89, 911
BseDI CCNNGG 2 cut(s) 240, 915
BseGI GGATG 5 cut(s) 84, 127, 532, 595, 946
BseLI CCNNNNNNNGG 2 cut(s) 795, 916
BseMII CTCAG 3 cut(s) 302, 714, 1146
BseNI ACTGG 2 cut(s) 1037, 1066
BseSI GKGCMC 1 cut(s) 746
BseXI GCAGC 4 cut(s) 378, 560, 764, 791
BseYI CCCAGC 1 cut(s) 77
BsgI GTGCAG 2 cut(s) 771, 798
Bsh1236I CGCG 1 cut(s) 596
BshFI GGCC 5 cut(s) 92, 126, 349, 662, 914
BshNI GGYRCC 1 cut(s) 1091
BshTI ACCGGT 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 833
BsiSI CCGG 4 cut(s) 165, 177, 566, 954
BslI CCNNNNNNNGG 2 cut(s) 795, 916
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 5 cut(s) 92, 126, 349, 662, 914
Bsp1286I GDGCHC 2 cut(s) 746, 833
Bsp143I GATC 6 cut(s) 314, 400, 703, 804, 1110, 1128
BspACI CCGC 2 cut(s) 596, 639
BspANI GGCC 5 cut(s) 92, 126, 349, 662, 914
BspCNI CTCAG 3 cut(s) 301, 713, 1145
BspFNI CGCG 1 cut(s) 596
BspLI GGNNCC 3 cut(s) 127, 348, 1093
BspMAI CTGCAG 1 cut(s) 550
BspMI ACCTGC 2 cut(s) 378, 718
BspPI GGATC 2 cut(s) 322, 698
BspT107I GGYRCC 1 cut(s) 1091
BsrFI RCCGGY 2 cut(s) 176, 953
BsrI ACTGG 2 cut(s) 1037, 1066
BssAI RCCGGY 2 cut(s) 176, 953
BssECI CCNNGG 2 cut(s) 240, 915
BssMI GATC 6 cut(s) 314, 400, 703, 804, 1110, 1128
BssT1I CCWWGG 2 cut(s) 240, 915
Bst2UI CCWGG 2 cut(s) 89, 911
Bst4CI ACNGT 3 cut(s) 37, 776, 1026
Bst6I CTCTTC 2 cut(s) 759, 902
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 932
BstDEI CTNAG 6 cut(s) 276, 288, 700, 995, 1132, 1148
BstF5I GGATG 5 cut(s) 84, 127, 532, 595, 946
BstFNI CGCG 1 cut(s) 596
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 6 cut(s) 317, 403, 706, 807, 1113, 1131
BstMBI GATC 6 cut(s) 314, 400, 703, 804, 1110, 1128
BstMWI GCNNNNNNNGC 2 cut(s) 140, 785
BstNI CCWGG 2 cut(s) 89, 911
BstPAI GACNNNNGTC 1 cut(s) 270
BstSCI CCNGG 3 cut(s) 87, 564, 909
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 746
BstSNI TACGTA 1 cut(s) 932
BstUI CGCG 1 cut(s) 596
BstV1I GCAGC 4 cut(s) 378, 560, 764, 791
BstV2I GAAGAC 1 cut(s) 466
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
BsuI GTATCC 1 cut(s) 1051
BsuRI GGCC 5 cut(s) 92, 126, 349, 662, 914
BtsCI GGATG 5 cut(s) 84, 127, 532, 595, 946
BtsIMutI CAGTG 4 cut(s) 42, 781, 1022, 1044
BveI ACCTGC 2 cut(s) 378, 718
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 2 cut(s) 176, 953
Cfr13I GGNCC 2 cut(s) 125, 347
CseI GACGC 4 cut(s) 523, 602, 866, 954
Csp6I GTAC 4 cut(s) 367, 457, 540, 772
CspAI ACCGGT 1 cut(s) 176
CviAII CATG 4 cut(s) 196, 229, 386, 884
CviQI GTAC 4 cut(s) 367, 457, 540, 772
DdeI CTNAG 6 cut(s) 276, 288, 700, 995, 1132, 1148
DpnI GATC 6 cut(s) 316, 402, 705, 806, 1112, 1130
DpnII GATC 6 cut(s) 314, 400, 703, 804, 1110, 1128
DriI GACNNNNNGTC 1 cut(s) 964
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 2 cut(s) 759, 902
Eam1105I GACNNNNNGTC 1 cut(s) 964
EarI CTCTTC 2 cut(s) 759, 902
Eco105I TACGTA 1 cut(s) 932
Eco130I CCWWGG 2 cut(s) 240, 915
Eco32I GATATC 1 cut(s) 451
Eco57I CTGAAG 2 cut(s) 95, 446
EcoRII CCWGG 2 cut(s) 87, 909
EcoRV GATATC 1 cut(s) 451
EcoT14I CCWWGG 2 cut(s) 240, 915
ErhI CCWWGG 2 cut(s) 240, 915
FaeI CATG 4 cut(s) 199, 232, 389, 887
FalI AAGNNNNNCTT 2 cut(s) 954, 986
FatI CATG 4 cut(s) 195, 228, 385, 883
FauI CCCGC 1 cut(s) 589
FbaI TGATCA 2 cut(s) 804, 1110
FblI GTMKAC 2 cut(s) 5, 843
Fnu4HI GCNGC 5 cut(s) 392, 549, 639, 753, 780
FokI GGATG 5 cut(s) 71, 134, 539, 602, 953
Fsp4HI GCNGC 5 cut(s) 392, 549, 639, 753, 780
FspBI CTAG 1 cut(s) 263
FspI TGCGCA 1 cut(s) 374
GlaI GCGC 1 cut(s) 374
GluI GCNGC 5 cut(s) 392, 549, 639, 753, 780
GsaI CCCAGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 893
HaeIII GGCC 5 cut(s) 92, 126, 349, 662, 914
HapII CCGG 4 cut(s) 165, 177, 566, 954
HgaI GACGC 4 cut(s) 523, 602, 866, 954
HhaI GCGC 1 cut(s) 375
Hin1II CATG 4 cut(s) 199, 232, 389, 887
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 4 cut(s) 6, 334, 877, 951
HindII GTYRAC 4 cut(s) 6, 334, 877, 951
HindIII AAGCTT 3 cut(s) 107, 518, 758
HinfI GANTC 7 cut(s) 101, 271, 382, 418, 604, 944, 991
HpaII CCGG 4 cut(s) 165, 177, 566, 954
HphI GGTGA 3 cut(s) 43, 181, 410
Hpy166II GTNNAC 6 cut(s) 6, 334, 844, 877, 891, 951
Hpy188I TCNGA 9 cut(s) 210, 291, 526, 703, 708, 868, 1110, 1135, 1151
Hpy188III TCNNGA 3 cut(s) 98, 263, 938
Hpy8I GTNNAC 6 cut(s) 6, 334, 844, 877, 891, 951
Hpy99I CGWCG 5 cut(s) 10, 721, 860, 962, 970
HpyAV CCTTC 6 cut(s) 470, 594, 993, 1076, 1082, 1134
HpyCH4III ACNGT 3 cut(s) 37, 776, 1026
HpyCH4IV ACGT 1 cut(s) 931
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 785
HpyF3I CTNAG 6 cut(s) 276, 288, 700, 995, 1132, 1148
HpySE526I ACGT 1 cut(s) 931
Hsp92II CATG 4 cut(s) 199, 232, 389, 887
HspAI GCGC 1 cut(s) 373
Ksp22I TGATCA 2 cut(s) 804, 1110
Kzo9I GATC 6 cut(s) 314, 400, 703, 804, 1110, 1128
LmnI GCTCC 3 cut(s) 211, 556, 828
Lsp1109I GCAGC 4 cut(s) 378, 560, 764, 791
LweI GCATC 4 cut(s) 121, 478, 703, 1093
MaeI CTAG 1 cut(s) 263
MaeII ACGT 1 cut(s) 931
MaeIII GTNAC 3 cut(s) 31, 187, 1041
MalI GATC 6 cut(s) 316, 402, 705, 806, 1112, 1130
MboI GATC 6 cut(s) 314, 400, 703, 804, 1110, 1128
MboII GAAGA 7 cut(s) 466, 520, 776, 889, 1000, 1042, 1112
MfeI CAATTG 3 cut(s) 116, 808, 1052
MflI RGATCY 1 cut(s) 703
MhlI GDGCHC 2 cut(s) 746, 833
MlsI TGGCCA 1 cut(s) 662
MluCI AATT 7 cut(s) 72, 116, 138, 159, 254, 808, 1052
MluNI TGGCCA 1 cut(s) 662
MlyI GAGTC 5 cut(s) 280, 376, 412, 598, 938
Mox20I TGGCCA 1 cut(s) 662
MscI TGGCCA 1 cut(s) 662
MseI TTAA 1 cut(s) 615
MslI CAYNNNNRTG 1 cut(s) 194
Msp20I TGGCCA 1 cut(s) 662
MspI CCGG 4 cut(s) 165, 177, 566, 954
MspR9I CCNGG 3 cut(s) 89, 566, 911
MunI CAATTG 3 cut(s) 116, 808, 1052
Mva1269I GAATGC 1 cut(s) 517
MvaI CCWGG 2 cut(s) 89, 911
MvnI CGCG 1 cut(s) 596
MwoI GCNNNNNNNGC 2 cut(s) 140, 785
NciI CCSGG 1 cut(s) 566
NdeII GATC 6 cut(s) 314, 400, 703, 804, 1110, 1128
NlaIII CATG 4 cut(s) 199, 232, 389, 887
NlaIV GGNNCC 3 cut(s) 127, 348, 1093
NmuCI GTSAC 3 cut(s) 31, 187, 1041
NsbI TGCGCA 1 cut(s) 374
PaqCI CACCTGC 1 cut(s) 718
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 2 cut(s) 101, 991
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 5 cut(s) 393, 550, 640, 754, 781
PleI GAGTC 5 cut(s) 279, 376, 412, 598, 938
PpsI GAGTC 5 cut(s) 279, 376, 412, 598, 938
Ppu21I YACGTR 1 cut(s) 932
PshAI GACNNNNGTC 1 cut(s) 270
Psp6I CCWGG 2 cut(s) 87, 909
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 2 cut(s) 87, 909
PspN4I GGNNCC 3 cut(s) 127, 348, 1093
PspPI GGNCC 2 cut(s) 125, 347
PstI CTGCAG 1 cut(s) 550
PsuI RGATCY 1 cut(s) 703
RsaI GTAC 4 cut(s) 368, 458, 541, 773
RsaNI GTAC 4 cut(s) 367, 457, 540, 772
RseI CAYNNNNRTG 1 cut(s) 194
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 615
SatI GCNGC 5 cut(s) 392, 549, 639, 753, 780
Sau3AI GATC 6 cut(s) 314, 400, 703, 804, 1110, 1128
Sau96I GGNCC 2 cut(s) 125, 347
SchI GAGTC 5 cut(s) 280, 376, 412, 598, 938
ScrFI CCNGG 3 cut(s) 89, 566, 911
SduI GDGCHC 2 cut(s) 746, 833
SfaNI GCATC 4 cut(s) 121, 478, 703, 1093
SfcI CTRYAG 1 cut(s) 546
SgrAI CRCCGGYG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 194
SnaBI TACGTA 1 cut(s) 932
Sse9I AATT 7 cut(s) 72, 116, 138, 159, 254, 808, 1052
SsiI CCGC 2 cut(s) 596, 639
SspMI CTAG 1 cut(s) 263
StyD4I CCNGG 3 cut(s) 87, 564, 909
StyI CCWWGG 2 cut(s) 240, 915
TaaI ACNGT 3 cut(s) 37, 776, 1026
TaiI ACGT 1 cut(s) 934
TaqI TCGA 4 cut(s) 5, 153, 298, 579
TasI AATT 7 cut(s) 72, 116, 138, 159, 254, 808, 1052
TatI WGTACW 1 cut(s) 771
TauI GCSGC 1 cut(s) 641
TfiI GAWTC 2 cut(s) 101, 991
Tru1I TTAA 1 cut(s) 615
Tru9I TTAA 1 cut(s) 615
TscAI CASTG 4 cut(s) 42, 781, 1029, 1044
TseFI GTSAC 3 cut(s) 31, 187, 1041
TseI GCWGC 4 cut(s) 391, 548, 752, 779
Tsp45I GTSAC 3 cut(s) 31, 187, 1041
TspDTI ATGAA 3 cut(s) 249, 872, 1113
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 4 cut(s) 42, 781, 1029, 1044
XapI RAATTY 1 cut(s) 254
XbaI TCTAGA 1 cut(s) 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmiI GTMKAC 2 cut(s) 5, 843
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.